Search

The heat capacity (Cp) of solid mixtures of silybin and sodium cholate was measured by a differential scanning calorimeter (DSC). X-ray diffraction and the FT-IR spectroscopy were used to determine the structure of solid mixture. Our results indicate that this binary system is composed of a mixture of two microcrystalline structures. The transformation of heat capacity from silybin to sodium cholate passes through a minimum at w1 = 0.25, where w1 is the mass fraction of sodium cholate. Polynomial equations of heat capacity versus temperature and the virial equation of excess heat capacity versus composition were applied to correlate experimental data. The changes of thermodynamic functions of enthalpy, entropy, and Gibbs function were calculated from the data of Cp.

The heat capacity C p of solid mixtures of silybin and poly(vinylpyrrolidone) (PVP) K30 was measured by differential scanning calorimetry (DSC). The IUPAC name for PVP is 1-ethenylpyrrolidin-2-one, and that for silybin is 3,5,7-trihydroxy-2-[3-(4-hydroxy-3-methoxyphenyl)-2-hydroxymethyl-2,3-dihydrobenzo-[1,4]dioxin-6-yl]-chroman-4-one. By analyzing the curves of (d C p /d T ) against temperature T , some apparent points, a maxima point and a minima point, to characterize the C p curve were obtained. The maxima point in the region of w 1 w 1 is the mass fraction of silybin. In this region, silybin is dispersed into the amorphous solid of PVP. The minima point in the region of w 1 > 0.4 is a character of crystalline silybin. In this region, a mixture of crystalline silybin and an amorphous solid dispersion is observed.

In this work, the density, viscosity, and electrical conductance of a ternary solution of {water + dextran + nicotinic acid} were determined experimentally. The data of electric conductance show that the dissociation of nicotinic acid is little influenced by the addition of dextran. The data of density indicate that with the addition of dextran a maximum of the solvation volume of nicotinic acid is observed. The B-coefficient of viscous flow was evaluated from viscosity data. Using Eyring’s transition state treatment, the activation parameters of viscous flow, which reflect the effect of the concentration of dextran on the motion of nicotinic acid, have been obtained. The result shows that dextran impedes the movement of the molecular nicotinic acid.

Solid dispersions and mixtures of quercetin and poly(vinylpyrrolidone) (PVP) K30 are studied by differential scanning calorimetry (DSC), X-ray powder diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), and heat capacity (C p ) measurements. The IUPAC name for PVP is 1-ethenylpyrrolidin-2-one, and that for quercetin is 2-(3,4-dihydroxyphenyl)-3,5,7-trihydroxy-4H-1-benzopyran-4-one. A solid dispersion is found in the region of w 1 0.5. where w 1 is the mass fraction of quercetin. The C p data is correlated to temperature T by a polynomial equation, and changes in enthalpy and entropy were calculated from C p . The C p data is valuable in the evaluation of drug efficiency.

Objective To investigate the expression level of E-cadherin in spinal plasma cell myeloma and its relationship with the clinicopathological indexes of spinal plasma cell myeloma. Methods The expression of E-cadherin was detected in 41 cases of spinal plasma cell myeloma and 14 cases of simple bone cysts by using S-P (streptavidin-perosidase) immunohistochemical staining. The relationship between E-cadherin expression and the clinicopathologica1 features was analyzed. Results In spinal plasma cell myelomas, the positive expression rate of E-cadherin (46.34%) was higher than that in simple bone cyst tissues (all negative) (P 0.05), but had relationship with the classification of light chain. The expression rate in lambda chain subgroup was lower than that in the others (P Conclusion E-cadherin expressed higher in spinal plasma cell myelomas than in simple bone cyst tissues and its expression had relationship with the light chain classification in spinal plasma cell myeloma. The patients with no stain of E-cadherin may have a poor prognosis. Detecting the type of light chain associated with expression of E-cadherin might be helpful in evaluating the outcome of spinal PCM patients.

The bioactive glass is used widely as bone-repairing material due to its excellent bioactivity, biocompatibility and the function for bone restoration, which depended, to a great extent, on the composition, microstructure of the glass and the process conditions. In this study, effect of process conditions (the melting temperature and time) on compositions, microstructure and the properties of bioactive glass was analyzed using EDS, Density measurement, XRD and FTIR techniques. The results indicated that compositions and microstructure changed because of the selective evaporation of constituent and the change in homogeneity of components.The bioactivities of the glasses prepared under different process conditions were characterized by immersing the glass particles in simulated body fluid (SBF) combined with FTIR technique. The results indicated that hydroxyl-carbonate-apatite (HCA) layer formed on the surface of the samples after 24 hours soaking in SBF. A quantitative method was used to compare the bioactivity of samples. It indicated the different preparation processes had influence on the bio-mineralization properties. Then the optimal preparation condition (1450°C, 30min) was obtained.

We examine the two-pion Hanbury-Brown-Twiss (HBT) interferometry for the particle-emitting source produced in heavy ion collisions at HIRFL-CSR energy. The source evolution is described by relativistic hydrodynamics with three kinds of equations of state for chemical equilibrium (CE), chemical freeze-out (CFO), and partial chemical equilibrium (PCE) models, respectively. We investigate the effects of particle decay, multiple scattering, and source collective expansion on the two-pion interferometry results. We find that the HBT radii of the evolution source for the CFO and PCE models are smaller than that for the CE model. The HBT lifetime for the CFO model is smaller than those for the PCE and CE models. The particle decay increases the HBT radius and lifetime while the source expansion decreases the HBT radius. The multiple scattering effect on the HBT results can be neglected based on our model calculations.

The sol-gel derived bioactive glass short fibers in the system CaO-P2O5-SiO2 was prepared using air-spray method. SBF immersion test indicated that the fibers possessed satisfactory bioactivity. SEM, XRD, FTIR analysis revealed that the morphologies and bioactivity of the fibers could be significantly influenced by the composition and viscosity of the solution. The fibers are very promising biomaterials for applications to bone restoration and tissue engineering as the bone defects fillers or additives for strengthening of the biomedical polymers.

We give the formulas of two-pion Hanbury-Brown-Twiss (HBT) correlation function for a partially coherent evolution pion-emitting source, using quantum probability amplitudes in a path-integral formalism. The multiple scattering of the particles in the source is taken into consideration based on Glauber scattering theory. Two-pion interferometry with effects of the multiple scattering and source collective expansion is examined for a partially coherent source of hadronic gas with a finite baryon density and evolving hydrodynamically. We do not find observable effect of either the multiple scattering or the source collective expansion on HBT chaotic parameter.

λ-Carrageenan is a seaweed polysaccharide which has been generally used as proinflammatory agent in the basic research, however, how the immunomodulating activity of λ-carrageenan affects tumor microenvironment remains unknown. In this study, we found that intratumoral injection of λ-carrageenan could inhibit tumor growth in B16-F10 and 4T1 bearing mice and enhance tumor immune response by increasing the number of tumor-infiltrating M1 macrophages, DCs and more activated CD4+CD8+ T lymphocytes in spleen. In addition, λ-carrageenan could enhance the secretion of IL17A in spleen and significantly increase the level of TNF-α in tumor, most of which was secreted by infiltrating macrophages. Moreover, λ-carrageenan exhibited an efficient adjuvant effect in OVA-based preventative and therapeutic vaccine for cancer treatment, which significantly enhanced the production of anti-OVA antibody. The toxicity analysis suggested that λ-carrageenan was with a good safety profile. Thus, λ-carrageenan might be used both as a potent antitumor agent and an efficient adjuvant in cancer immunotherapy.

RNA modifications, especially methylation of the N6 position of adenosine (A)--m6A, represent an emerging research territory in RNA biology. m6A is a post-transcriptional modification of RNAs, which is catalyzed by the mRNA: m6A methyltransferase complex containing three individual components and is the most common form found in the internal sequences of mRNAs in eukaryotes. Latest study showed that the fat mass and obesity-associated protein could remove the methyl group, indicating that the modification is reversible. Importantly, inhibiting or silencing the methyltransferase will cause significant changes of phenotypes. However, due to limited detection methods, the mechanism of m6A has not been figured out yet. Next-generation sequencing combining with IP (immunoprecipitation) technologies makes it possible to detect m6A modifications in a large scale. Here, we reviewed recent progresses of m6A studies including the discovery of m6A, mechanism of biosynthesis, tissue and genome distribution, detection methodology and possible biological functions. We also compared three IP-seq technologies that are currently widely used, and summarized the challenges in m6A studies.

Lipopolysaccharide (LPS) is a major component of the outer surface membrane of Gram-negative bacteria which has been proved an effective immune enhancer. Here, we investigated the anti-tumor effect of irradiated tumor cells that stimulated by LPS in mouse xenografts models.Tumor cells were irradiated after stimulation with 1 μg/mL LPS for 48 h. The C57BL/6 mice were immunized subcutaneously with irradiated tumor cells. The anti-tumor effect of lymphocytes of immunized mice was investigated. The cytotoxicity of spleen lymphocytes from immunized mice was determined by a standard (51)Cr-release assay. The roles of immune cell subsets in anti-tumor activity were assessed by injected intraperitoneally with monoclonal antibodies.We observed that the vaccine of irradiated tumor cell with LPS-stimulated elicited a stronger protective anti-tumor immunity than other controls. Adoptive transfer of lymphocytes of immunized mice showed that the cellular immune response was involved in the anti-tumor effect. And this effect was achieved by activation of antigen-specific CD8(+) T cell response and reduction of myeloid-derived suppressor cells (MDSCs, Gr1(+) CD11b (+) ), which were confirmed by depletion of immune cell subsets and flow cytometry analysis.In summary, our study showed that stimulation of LPS was able to enhance anti-tumor immunity of vaccination with tumor cells after irradiation treatment, which might be a new strategy for cancer therapy.

Xiang Gao,1,2 Xiaohui Deng,3 Xiawei Wei,2 Huashan Shi,2 Fengtian Wang,2 Tinghong Ye,2 Bin Shao,2 Wen Nie,2 Yuli Li,2 Min Luo,2 Changyang Gong,2 Ning Huang1 1Department of Pathophysiology, College of Preclinical and Forensic Medical Sciences, Sichuan University, Chengdu, 2State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, West China Medical School, Sichuan University, Chengdu, 3Department of Human Anatomy, Xinxiang Medical University, Xinxiang, People’s Republic of China Abstract: Adhesions can form after almost any type of abdominal surgery. Postoperative adhesions can be prevented by improved surgical techniques, such as reducing surgical trauma, preventing ischemia, and avoiding exposure of the peritoneal cavity to foreign materials. Although improved surgical techniques can potentially reduce formation of adhesions, they cannot be eliminated completely. Therefore, finding more effective methods to prevent postoperative adhesions is imperative. Recently, we found that a novel thermosensitive hydrogel, ie, poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone) (PCEC) had the potential to prevent postoperative adhesions. Using the ring-opening polymerization method, we prepared a PCEC copolymer which could be dissolved and assembled at 55°C into PCEC micelles with mean size of 25 nm. At body temperature, a solution containing PCEC micelles could convert into a hydrogel. The PCEC copolymer was biodegradable and had low toxicity in vitro and in vivo. We found that most animals in a hydrogel-treated group (n = 10) did not develop adhesions. In contrast, 10 untreated animals developed adhesions that could only be separated by sharp dissection (P < 0.001). The hydrogel could adhere to peritoneal wounds and degraded gradually over 7–9 days, transforming into a viscous fluid that was completely absorbed within 12 days. The injured parietal and visceral peritoneum remesothelialized over about seven and nine days, respectively. This study confirms that PCEC hydrogel has potential application in the prevention of postoperative adhesions. Keywords: poly(ε-caprolactone)-poly(ethylene glycol)-poly(ε-caprolactone), thermosensitive, biodegradable, hydrogel, postoperative adhesions

To investigate the proliferation and differentiation of the human dental pulp cells (hDPCs) on the bioactive scaffolds.Primary HDPCs were harvested from impacted third molars of healthy adult individuals (18-25 years of age) by enzyme digestion, expanded and cultured. The cells used for this investigation were the 4 th passage. Immunohistochemical methods were used to verify that the cells were dental pulp cells. The expression of stromal precursor antigen-1 (STRO-1) was determined by flow cytometry. Three different types of scaffolds were used: collagen (COL), collagen / bioglass (COL-BG) and collagen / bioglass / polycaprolactone (COL-BG-PCL). Cell proliferation on the scaffolds was determined using a MTT assay at hour 6, on days 1, 3, 5, 7, 14 and 21. On day 14, the scaffolds were stained with the alkaline phosphatase (ALP) staining kit.The tested cells had STRO-1 positive cells. The proliferation of HDPCs was significantly higher on the COL-BG scaffold and COL-BG-PCL scaffold as compared with COL scaffold (P0.05). Especially on days 14 and 21, the optical density value of bioglass composite scaffold were 5 times that of the COL scaffold. The ALP positive staining area was observed more extensively on the COL-BG scaffold and COL-BG-PCL scaffold than on the COL scaffold.As comparison with the COL scaffold, HDPCs' proliferation and differentiation present more activity on the COL-BG and COL-BG-PCL scaffolds.

A series of novel riminophenazine derivatives bearing an alkyl substituent attached to N-5 and imino nitrogen at C-3 position of the phenazine ring were obtained through rational drug design, aiming to maintain high anti-tubercular activity, lower toxicity and reduce lipophilicity. All target compounds were prepared by utilizing simple and flexible synthetic route and evaluated against Mycobacterium tuberculosis H37Rv and screened for mammalian cytotoxicity. The results demonstrated that compounds with a cyclopropyl substituent at N-5 position were more active than the reference compound clofazimine. In particular, 2-(4-chloroanilino)-5-cyclopropyl-3-(4-methoxycyclohexyl) imino-3, 5-dihydrophenazine (25) was found to be the most potent compound with low cytotoxicity and lipophilicity. This compound could serve as a valuable lead molecule for further optimization.

To investigate the different expression of various subtypes of human leukocyte antigen-G (HLA-G) in placenta of patients complicated with severe pre-eclampsia.Ten placental samples from early-onset severe pre-eclamptic pregnancies and ten from late-onset severe pre-eclamptic pregnancies were collected as study group; ten placental samples from preterm pregnancies and ten from normal pregnancies were collected as control group. The levels of HLA-G protein in the four groups were measured by western blot and immunohistochemistry.(1) HLA-G1 protein decreased significantly in both the early-onset (2.4 ± 0.6 versus 2.9 ± 1.1, P0.05) and the late-onset pre-eclampsia groups (3.5 ± 2.1 versus 4.2 ± 2.4, P0.05). (2) HLA-G5 protein increased in the late-onset pre-eclampsia groups (1.8 ± 1.1 versus 1.1 ± 0.9, P0.05); the increase in the early-onset pre-eclampsia group is not obvious (1.6 ± 0.9 versus 1.4 ± 0.7, P0.05). (3) The level of HLA-G1 protein in placenta from patients complicated with premature labor is lower (2.9 ± 1.1 versus 4.2 ± 2.4, P0.05); HLA-G5 protein does not change significantly (1.4 ± 0.7 versus 1.1 ± 0.9, P0.05). (4) HLA-G1 and G5 proteins mainly express in the placenta extravillous cytotrophoblast cells. There is also a high level of expression around the blood vessels and in the extraembryonic mesoderm.(1) HLA-G1 decreased significantly in both the early-onset and late-onset pre-eclamptic patients. (2) HLA-G5 increased in both the early-onset and late-onset pre-eclamptic patients, and the increase in the late-onset pre-eclamptic patients is obvious. (3) In late pregnancy, the level of HLA-G1 is lower in patients complicated with premature labor, this may be the result of its earlier pregnancy week; HLA-G5 does not change significantly. (4) HLA-G1 and G5 mainly express in the placenta extravillous cytotrophoblast cells.

Dear Editor: I am Dr Xiao-Mei Ma, from the Department of Pathology in Changzheng Hospital at the Second Military Medical University, Shanghai, China. We present here a case report of a seminal vesicle cyst (SVC) in a young man with epididymal tissue in a dysgenetic kidney and ectopic ureteral insertion. SVCs are usually found in the second to fourth decades of life. SVCs can be congenital or acquired. Congenital SVCs seem to be invariably associated with renal dysplasia, hypoplasia, agenesis and duplication abnormalities in the same side, with the exception of two cases 1, 2. We report here a case report of a 26-year-old man who was referred to our hospital owing to absence of the left kidney, identified by sonography during a health examination. Magnetic resonance imaging (MRI) and magnetic resonance urography (MRU) showed a cyst expanding from the kidney fossa to the seminal vesicle fossa instead of the left kidney, ureter and seminal vesicle (Figure 1). His right urinary tract was normal. A solid mass was present in the left side of the bladder, and the left ureteral orifice could not be seen with a cystoscope. Laboratory tests of the blood and urine were normal. Clinical examination showed that the patient had a normal pair of testes, epididymis and penis. Figure 1 Magnetic resonance imaging (MRI) revealed that there was a cyst from the kidney fossa to the seminal vesicle fossa. (A): The seminal vesicle was cystic and compressed the bladder from outside (arrow pointing downward). The dilated ureter entered the expanded ... The surgeons performed a retroperitoneoscopy together with a small inclined inferior abdominal incision under general anaesthesia. At first, the patient was recumbent on his right arm. The cyst was resected in the postperitoneal region. There were three laparoscopic ports: a 12-mm trocar port at the left postaxillary line below the 12th rib, a 5-mm trocar port at the preaxillary line and 2 cm above the spina iliaca and a 10-mm trocar port at the preaxillary line below the arcus costarum. During the operation, the surgeons noticed that the cyst was just as shown by MRI and MRU. There were no vessels to the left kidney. The cyst was dissociated easily. The ectopic ureter opened in the seminal vesicle cyst, which was filled with chyliform fluid. At first, the surgeons suspected that the fluid was liquor puris, but it was later found to be semen with few sperm, which had poor vitality. Thereafter, the patient was moved to the Trendelenburg position. A small inclined inferior abdominal incision was made at the left lateral border of the rectus muscle. This cyst was resected completely. The total operating time was 120 min, with an estimated blood loss of 100 mL. The patient was able to eat and walk within 24 h after the operation. Macroscopically, the specimen was a membranous cyst filled with turbid fluid (Figure 2). It was 21.0 cm long and 1.5–6.0 cm in diameter. Figure 2 The specimen was a membranous cyst. One edge was the agenetic left kidney (arrowhead). Next to the agenetic left kidney was the dilated ureter (long arrow) and the seminal vesicle (short arrow). The image obtained by microscopy of the surgical specimen ... Microscopically, there were glomerulus, renal tubules and expanded epididymal ducts filled with abnormal sperm in the dysgenetic kidney (Figure 3). The dilated ureter was lined by urothelial epithelium. The seminal vesicle cyst wall consisted of a muscular layer lined by urothelial epithelium. Thus, the pathological diagnosis was epididymal tissue in the dilated portion of the dysgenetic kidney with an ipsilateral seminal vesicle cyst and ectopic ureteral insertion. Figure 3 (A): A glomerulus and renal tubules (arrows pointing right), as well as expanded epididymal ducts filled with abnormal sperm (arrow pointing left), were in the wall of the agenetic kidney (hematoxylin and eosin, original magnification, × 40). ... The patient recovered quickly and fathered a child who was born 2 years later. In this case, the patient had no history of infection. The left ureteral orifice was not in the bladder, but was inserted into the SVC. We found glomerulus and renal tubules in the specimen using microscopy. Those findings confirmed that the SVC of the patient was not acquired, but congenital, and that the left kidney was dysplastic. Until now, there have been only 27 papers about SVCs with renal dysplasia of a kidney bud 3 in the PubMed literature. There was a report of ectopic prostate glands and urothelial epithelium in a seminal vesicle cyst 4. This is the only case in the literature with epididymal tissue in the dysplastic kidney. The dysplastic kidney in our patient was a cystic with a thin wall. The cyst described in this case was probably the largest one out of all of the published cases. Seminal vesicle cysts accompanying renal dysplasia may be related to the dysregulation of the embryonic development of the distal mesonephric duct, which develops into the genitourinary system in humans. Thus, urinary malformations are associated with two-thirds of all the SVC cases, consisting mostly of ectopic ureteral insertion and ipsilateral renal dysgenesis/agenesis, and the epididymis developing from the mesonephric duct may partly reside in the kidney, as in this case. Sometimes there is a mass in the bladder because the SVC is compressing it from the outside. Surgeons usually misdiagnose such a mass as a tumour. There have indeed been reports of SVC associated with carcinoma of the bladder 5. After years of sexual activity, secretions reach the maximum level and are drained into the SVC, the ureter and the agenetic/dysgenetic kidney, which cause the cyst to fill with seminal fluid. The sperms are inactive or immature. Thus, the patients are usually infertile. Surgery is always a successful treatment for this condition, especially for patients with symptoms 6. There is a great danger of recurrence or infection in conventional treatment methods. Radical resection is effective. Laparoscopy together with small incision through a transperitoneal approach has gained acceptance because of the absolute advantages of lower postoperative pain, lower morbidity, shorter hospital stay and shorter convalescence. The use of laparoscopy for the management of a large seminal vesicle cyst with ipsilateral renal agenesis has been previously reported. Han et al. 7 reported a patient with a stone within the SVC; this patient was treated successfully with laparoscopic surgery. Taken together with our case, these reports indicate that the laparoscopic surgery may be the first choice for SVC patients who must have surgical treatment. However, the rarity of this condition makes extensive study of such cases impossible.

To find out how GATA-1 and GATA-2 behave in the bone marrow of patients with Monge's disease.The levels of mRNA in mononuclear cells (MNC) and proteins of GATA-1 and GATA-2 in the bone marrow of patients with Monge's disease and controls were determined by RT-PCR and immune cytolysis chemical method.(1) All patients and controls expressed GATA-1 mRNA (Monge's disease 1.033 +/- 0.146, Control 0.458 +/- 0.076) and GATA-2 mRNA (Monge's disease 0.451 +/- 0.073, Control 0.185 +/- 0.074). All patients expressed both GATA-1 (positive cell counts 77.3 +/- 33.3, positive score 135.4 +/- 75.4) and GATA-2 ( positive cell counts 29.4 +/- 11.4, positive score 48.4 +/- 19.7). All the controls expressed GATA-1 (positive cell counts 18.1 +/- 11.3, positive score 24.2 +/- 13.4) while 12 of 20 controls expressed GATA-2 ( positive cell counts 5.4 +/- 3.0, positive score 7.3 +/- 4.2). The expression of mRNA and proteins of GATA-1 and GATA-2 in Monge's disease were higher than in controls (P0.01). (2) There was a positive correlation between GATA-1 and Hb (P0.01), as did between mRNA and proteins of GATA-1 and GATA-2. (3) Both the proteins of GATA-1 and GATA-2 located only in the cytoplasm but not the nucleus.Two of inherent genes, GATA-1 and GATA-2 which were expressed at higher levels in patients with Monge's disease than in controls might play significant roles in the pathogenesis of Monge's disease.

In Pawlak's rough set system, approximation quality can be used to measure the classification capability of a condition attribute and can be used to define the significance of condition attributes. But the measure only gives us the determinate classification capability, and does not give us the uncertain classification capability. The information entropy is a mean value in terms of probability that embodies the whole information of an attribute, measuring the mean classification capability. The definition of mutual information is not intuitionistic and not easily understood; the calculation of the mutual information is somewhat complicated too. In this paper, we give a definition of probability equivalence between condition and decision attributes, and based on the probability equivalence we give a measure of significance of a condition attribute. An Illustrative example shows that with it the optimal attribute reduction can be gotten.

AIM: To detect the expression of Fas ligand (FasL) in colon cancer tissues and cell lines and analyze the function of FasL-expressing colon cancer cells in inducing Fas-sensitive T lymphocyte apoptosis. METHODS: Ninety surgically resected colon cancer tissues and 15 hepatic metastasis specimens were investigated by immunohistochemical method with normal colon mucosa and colon adenoma as control. The relationship between FasL expression and pathologic features was also analyzed. FasL expression of 4 colon cancer cell lines, SW620, Lovo, LS-174T and SW1116, were detected by Western blotting assay. The function of FasL expressed on colon cancer cells was determined by coculture assay with Jurkat T lymphocytes, the apoptotic rate of which was detected by flow cytometry assay. RESULTS: Fifty-six (62.22%) cases of all the 90 colon cancer tissues and all (100%) the liver metastasis specimens expressed FasL, significantly higher than normal colon mucosa and colonic adenoma. Higher expression of FasL was found in more advanced stage of colon cancer and in cancer tissues with lymphatic or hepatic metastasis. All the colon cancer cell lines were found to express FasL. After coculture with the SW1116 cells for 24 h with an effector: target ratio 10:1, the rate of apoptosis of Jurkat cells rose from 1.9% to 21.0%. CONCLUSION: The expression of FasL is upregulated in colon cancer and the functionally expressed FasL can induce apoptosis of Fas-expressing T lymphocytes.

To make immunopathological study of hepatitis B virus transgenic mice line C57-TgN (HBV adr2.0) SMMU.Twenty transgenic C57-TgN mice of SPF grade and corresponding control mice C57BL/6 were used for the study. The expressions of CD3, CD4 and CD8 on lymphocytes in peripheral blood from transgenic and normal C57BL/6 mice were detected by flow cytometry. T lymphocyte subsets in the liver tissues from transgenic mice and hepatitis B patients were analyzed by EnVision staining.The expression of CD3, CD4 and CD8 on lymphocytes in peripheral blood from transgenic mice was lower than those from normal mice. The infiltrative mononuclear cells in the liver tissues of transgenic mice were mainly CD3+ CD4+ cells, and no CD8+ CD57+ cells was found. The infiltrative mononuclear cells in the liver tissues of human hepatitis B patients were mainly CD3+ CD4+ or CD3+ CD8+ cells.T cell subsets in the peripheral blood and liver tissues of transgenic mice C57-TgN (adr2.0) SMMU are different from those in human chronic hepatitis B patients.