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Abstract Introduction Targeted therapies are based on specific gene alterations. Various specimen types have been used to determine gene alterations, however, no systemic comparisons have yet been made. Herein, we assessed alterations in selected cancer‐associated genes across varying sample sites in lung cancer patients. Materials and Methods Targeted deep sequencing for 48 tumor‐related genes was applied to 153 samples from 55 lung cancer patients obtained from six sources: Formalin‐fixed paraffin‐embedded (FFPE) tumor tissues, pleural effusion supernatant (PES) and pleural effusion cell sediments (PEC), white blood cells (WBCs), oral epithelial cells (OECs), and plasma. Results Mutations were detected in 96% (53/55) of the patients and in 83% (40/48) of the selected genes. Each sample type exhibited a characteristic mutational pattern. As anticipated, TP53 was the most affected sequence (54.5% patients), however this was followed by NOTCH1 (36%, across all sample types). EGFR was altered in patient samples at a frequency of 32.7% and KRAS 10.9%. This high EGFR/ low KRAS frequency is in accordance with other TCGA cohorts of Asian origin but differs from the Caucasian population where KRAS is the more dominant mutation. Additionally, 66% (31/47) of PEC samples had copy number variants (CNVs) in at least one gene. Unlike the concurrent loss and gain in most genes, herein NOTCH1 loss was identified in 21% patients, with no gain observed. Based on the relative prevalence of mutations and CNVs, we divided lung cancer patients into SNV‐dominated, CNV‐dominated, and codominated groups. Conclusions Our results confirm previous reports that EGFR mutations are more prevalent than KRAS in Chinese lung cancer patients. NOTCH1 gene alterations are more common than previously reported and reveals a role of NOTCH1 modifications in tumor metastasis. Furthermore, genetic material from malignant pleural effusion cell sediments may be a noninvasive manner to identify CNV and participate in treatment decisions.

The Lidang circular structure in the center of the Guangxi Province is about 8 km in diameter. This structure appears as an abnormal shallow depression that has disturbed the rather harmonic regional joint systems. Its unique occurrence in the whole region, the circular morphology, negative topography, and the spatial distribution of interior and exterior strata are all consistent with those of impact craters that are formed by asteroidal or cometary collision. To test the impact hypothesis, we carried out both field investigation and remote sensing study of this structure. Regional geological history suggests that if the impact hypothesis were correct, the impact event should have occurred at or after the Early Permian. Field investigation found that the strata inside and outside the crater are dominated by parallel stacks of Lower and Upper Permian limestone that have various thicknesses and different mud contents. The layers of limestone within and outside the circular structure have identical attitudes; no structural disturbances were visible in the outcrops. Field investigations provide conclusive evidence against the impact cratering hypothesis. A high-resolution digital elevation model shows that the spatial distribution of rounded mountains within the structure is controlled by faint but continual extension of joints, suggesting that the crater interior has gone through a much higher degree of erosion. Therefore, regional joints that had once existed within the crater are preserved less well than exterior terrains, forming the abruptly disrupted circular depression. Differential erosion, as the possible formation mechanism of the Lidang structure, is consistent with the different mud contents found between the interior and exterior limestone. The circular outline of this structure may correspond to the shape of the original deposition basin. In conclusion, the Lidang circular structure is a polje formed by karstification, not an astrobleme.

Summary: Ammonia is one of the most important bulk chemicals in modern society. However, the highly energy-extensive contemporary industrial production of ammonia was developed in the early 20th century and requires extensive heating of highly pressurized flammable hydrogen gas, whose global production still relies heavily on non-sustainable petroleum. The development of “sustainable” nitrogen fixation process represents a grand aspirational chemical pursuit concerning our future human well-being. Herein, we report an ultra-stable nitride-based photosensitizing semiconductor that enables efficient, sustainable, and mild photochemical nitrogen fixation. The catalyst exhibits strong chemisorption of nitrogen and enables immediate electron donation from its surface vacancy to nitrogen. In addition, it was also demonstrated that the nitride-based semiconductor possesses the potential to minimize electron-hole recombination. : Chemical Reaction; Catalysis; Density Functional Theory (DFT); Nanostructure Subject Areas: Chemical Reaction, Catalysis, Density Functional Theory (DFT), Nanostructure

China experiences severe particulate pollution, especially in winter, and determining the characteristics of particulate matter (PM) during pollution events is imperative for understanding the sources and causes of the pollution. However, inconsistencies have been found in the aerosol composition, sources and secondary processing among reported studies. Modern meta-analysis was used to probe the PM chemical characteristics and processing in winter at four representative regions of China, and the first finding was that secondary aerosol formation was the major effect factor for PM pollution. The secondary inorganic species behaved differently in the four regions: sulfate, nitrate, and ammonium increased in the Beijing–Tianjin–Hebei (BTH) and Guanzhong (GZ) areas, but only nitrate increased in the Pearl River Delta (PRD) and Yangtze River Delta (YRD) regions. The increased production of secondary organic aerosol (SOA) was probably caused by aqueous-phase processing in the GZ and BTH regions and by photochemical reactions in the PRD. Finally, we suggest future AMS/ACSM observations should focus on the aerosol characteristics in rural areas in winter in China. Keywords: Secondary aerosols, Aqueous-phase reactions, Photochemical reactions, Polluted days, Winter

Abstract Background In China, unmarried female migrants are vulnerable to sexual and reproductive health risks. One effective protection strategy is promoting consistent condom use (CCU). Methods We conducted a cross-sectional study to apply the information-motivation-behavioral skills (IMB) model and modified it by addition of psychological and personal factors to examine the related factors of CCU and provide suggestions for intervention among unmarried female migrants. Results Of all 903 eligible participants, only 13.8% of participants reported CCU in the past six months. Both the IMB model and the modified IMB model provided acceptable fit to the data. In both models, information had no direct or indirect influence on CCU (p > 0.05). However, behavioral skills had a positive effect on CCU (β = 0.344, p 0.05). In the modified model, psychological and personality factors influenced CCU directly (β = − 0.100, p = 0.005). Conclusions Our results highlight the importance of conducting CCU promotion among unmarried female migrants. Future intervention strategies should focus on both the traditional IMB model constructs and the added psychological and personality factors.

The online optimal transmission policy and outage performance are investigated for energy harvesting (EH) two-way relay (TWR) networks by exploiting a real-data-driven stochastic EH model. In this network, two solar-powered source nodes are both equipped with finite-sized batteries, and communicate with each other via a relay node. Two energy supply modes are studied for the relay node: fixed power (FP) or solar-powered as an EH node with a finite-sized battery. The long-term outage probability of the TWR network is optimized by adapting the transmission power of all EH nodes to the causal solar EH condition, the fading channel states and the energy storage of their batteries. Thus, the framework is formulated as a Markov decision process (MDP), and the reward function is defined as the complement of the network's conditional outage probability, whose exact closed-form is derived theoretically. Based on the MDP model, the optimal transmission policy and expected outage probability of the network are computed. In the FP relay mode, a two-dimensional (2D) on-off threshold structure of the optimal policy is revealed, and it shows the 2-D zero-action, non-conservative and power-converged properties of the power actions of the two EH source nodes. This special property indicates the transmission interaction between the two EH source nodes. Further, the outage performance limit of the network is found out to be determined by the deficiency probability of network batteries, and it reflects the impact of the EH capabilities of the two source nodes on the network performance. Moreover, in the EH relay mode, a more complicated three-dimensional on-off threshold structure of the optimal policy and the outage performance limit of the network are also derived, reflecting the impact of the EH relay on the network's power allocation and outage performance, respectively. Finally, computer simulations are demonstrated to verify the theoretical analysis.

The optimal transmission policy is investigated for two-way (TW) energy harvesting (EH) dual-relay networks with a stochastic EH model. There exist two solar-powered relays which have finite-sized batteries, and at most one relay is selected to facilitate the network's information exchange. The objective is to optimize the network's long-term outage performance by adapting the relay selection and power allocation to the system stochastic conditions. Thus, a Markov decision process (MDP) is utilized to formulate the design framework. From the MDP, the optimal transmission policy of the network is derived to indicate the adaptive relay selection and power allocation, and the computation of the network's expected outage probability is presented. Further, the optimal transmission policy is asymptotically analyzed, and an active relaying property and a power convergency property of the optimal policy are pointed out. Moreover, according to the properties of the optimal policy, the long-term outage probabilities of the network are found to approach the limitation in sufficiently high SNRs, which is determined by the empty probability of the dual-battery under the optimal policy. Finally, computer simulations validate the analysis.

Thanks to the boom of computer vision techniques and artificial intelligence algorithms, it is more available to achieve artificial rearing for animals in real production scenarios. Improving the accuracy of chicken day-age detection is one of the instances, which is of great importance for chicken rearing. To solve this problem, we proposed an attention encoder structure to extract chicken image features, trying to improve the detection accuracy. To cope with the imbalance of the dataset, various data enhancement schemes such as Cutout, CutMix, and MixUp were proposed to verify the effectiveness of the proposed attention encoder. This paper put the structure into various mainstream CNN networks for comparison and multiple ablation experiments. The final experimental results show that by applying the attention encoder structure, ResNet-50 can improve the accuracy of chicken age detection to 95.2%. Finally, this paper also designed a complete image acquisition system for chicken houses and a detection application configured for mobile devices.

On the planet Mercury, pyroclastic deposits formed by explosive volcanism are developed around rimless volcanic pits that are up to dozens of kilometers in diameters. Some pyroclastic deposits on Mercury, however, host no discernable main eruption centers but feature pitted-ground terrains that each consists of many similar sized and irregularly shaped pits. Individual pits are usually much smaller and shallower than typical volcanoes on Mercury. The origin of these landforms is unknown, but it is indicative of styles of volcanism on Mercury and/or post-volcanic modifications. Here, we investigate the possible origin of these peculiar landforms based on their geological context, morphology, geometry, reflectance spectra, and geophysical background. Reflectance spectra of pyroclastic deposits around such volcanoes are comparable with those erupted from typical volcanic pits on Mercury, suggesting a genetic relation between these pitted-ground terrains with explosive volcanism, and the source magma might have similar compositions. Pitted-ground volcanoes are mainly observed in impact structures, and two cases were formed in high-reflectance smooth plains and channeled lava flows. Most pitted-ground volcanoes are relatively degraded compared with typical volcanoes on Mercury, and some might have been formed in geological recent times judged by both their pristine preservation and crosscutting relationship with impact rays. All pitted-ground volcanoes have unconfined morphology boundaries, and each case is composed by dozens of rimless pits that have similar preservation states and interconnected edges. Such morphological characteristics are unique among volcanic landforms on terrestrial bodies, and they cannot be explained by multiple post-eruption collapses of a main explosive volcano. Pitted-ground volcanoes that are developed in lava flows with the same age have different preservation states, suggesting that the pits were not formed by escape of thermally destabilized volatiles from substrate and subsequent roof collapses. The largest pitted-ground volcano (~3700 km2) is located on the Borealis Planitia, and Bouguer gravity data reveal no larger mass concentration in the subsurface than surrounding terrains, consistent with a paucity of shallow intrusions in the crust of Mercury. Short-term and spatially-clustered explosive eruptions could explain the peculiar morphology and geometry of the pits, suggesting that pits in a given pitted-ground volcano are akin to swarms of monogenetic volcanoes. However, possible magma dynamics for the formation of pitted-ground volcanoes cannot be confirmed until future high-resolution gravity mapping could reveal detailed interior structures beneath these volcanoes. Based on comparative studies with spatially-clustered and similarly aged volcanoes on Earth, we interpret that a combination of pervasive crustal fractures and regional thermal anomaly in the thin mantle of Mercury might have caused such short-term and spatially-clustered explosive eruptions. If this interpretation was true, the heavy degradation state of most pitted-ground volcanoes and the few well-preserved cases are consistent with an overall cooling trend of the mantle, indicating the existence of longstanding heterogeneous thermal structures in the mantle.

As one of the most frequently occurring malignancies in women, breast cancer (BC) is still an enormous threat to women all over the world. The high mortality rates in BC patients are associated with BC recurrence, metastatic progression to distant organs, and therapeutic resistance. Circular RNAs (circRNAs), belonging to the non-coding RNAs (ncRNAs), are connected end to end to form covalently closed single-chain circular molecules. CircRNAs are widely found in different species and a variety of human cells, with the features of diversity, evolutionary conservation, stability, and specificity. CircRNAs are emerging important participators in multiple diseases, including cardiovascular disease, inflammation, and cancer. Recent studies have shown that circRNAs are involved in BC progress by regulating gene expression at the transcriptional or post-transcriptional level via binding to miRNAs then inhibiting their function, suggesting that circRNAs may be potential targets for early diagnosis, treatment, and prognosis of BC. Herein, in this article, we have reviewed and summarized the current studies about the biogenesis, features, and functions of circRNAs. More importantly, we emphatically elucidate the pivotal functions and mechanisms of circRNAs in BC growth, metastasis, diagnosis, and drug resistance. Deciphering the complex networks, especially the circRNA-miRNA target gene axis, will endow huge potentials in developing therapeutic strategies for combating BC.

Abstract Kidney damage initiates the deteriorating metabolic states in tubule cells that lead to the development of end‐stage renal disease (ESTD). Interleukin‐22 (IL‐22) is an effective therapeutic antidote for kidney injury via promoting kidney recovery, but little is known about the underlying molecular mechanisms. Here, we first provide evidence that IL‐22 attenuates kidney injury via metabolic reprogramming of renal tubular epithelial cells (TECs). Specifically, our data suggest that IL‐22 regulates mitochondrial function and glycolysis in damaged TECs. Further observations indicate that IL‐22 alleviates the accumulation of mitochondrial reactive oxygen species (ROS) and dysfunctional mitochondria via the induction of AMPK/AKT signaling and PFBFK3 activities. In mice, amelioration of kidney injury and necrosis and improvement of kidney functions via regulation of these metabolism relevant signaling and mitochondrial fitness of recombinant IL‐22 are certificated in cisplatin‐induced kidney damage and diabetic nephropathy (DN) animal models. Taken together, our findings unravel new mechanistic insights into protective effects of IL‐22 on kidneys and highlight the therapeutic opportunities of IL‐22 and the involved metabolic regulators in various kidney diseases.

Tetanus is a life-threatening infectious neurological disorder that is now a rare disease due to the institution of wide-spread vaccination strategies. We present an uncommon case of generalized severe tetanus with consequent respiratory failure requiring mechanical ventilation, which was associated with dysautonomia. A 20-year-old unvaccinated female presented with neck stiffness and diffuse muscle spasms following a laceration sustained 3 weeks prior. She was admitted to the intensive care unit for mechanical ventilation and was treated with immunoglobulin, tetanus toxoid, metronidazole, and high doses of sedatives. She also developed dysautonomia, with alternating bradycardia and tachycardia, as well as fluctuating blood pressure. She was successfully extubated and discharged. We also review the epidemiology, pathophysiology, and management of tetanus and discuss dysautonomia in the setting of tetanus.

In this paper, we consider the difference in the abstraction level of features extracted by different perceptual layers and use a weighted perceptual loss-based generative adversarial network to deblur the UAV images, which removes the blur and restores the texture details of the images well. The perceptual loss is used as an objective evaluation index for training process monitoring and model selection, which eliminates the need for extensive manual comparison of the deblurring effect and facilitates model selection. The UNet jump connection structure facilitates the transfer of features across layers in the network, reduces the learning difficulty of the generator, and improves the stability of adversarial training.

Water ionization is an efficient physical water treatment technology, and crop water and nutrient use efficiencies can be improved using ionized water for irrigation. In order to explore the effect of ionized water on soil nitrification and nitrifying microorganisms, we conducted a laboratory soil incubation experiment with the addition of ionized water and ordinary water under different soil water contents (equal to 30%, 60%, 100% and 175% of the field capacity, θFC). During the soil incubation, we analyzed soil inorganic nitrogen transformation, ammonia oxidation gene abundances and nitrifying microbial community structure. The results showed that, no matter adding ordinary water or ionized water, the soil nitrification rate and the abundance of ammonia oxidizing bacteria in the 100%θFC treatment were significantly higher than those in other water conditions, while the abundance of ammonia oxidizing archaea was not affected by the soil water content. With the same soil water content, the nitrification rate of ionized water treatment was stronger than that of the ordinary water treatment. Although the absolute abundance of ammonia-oxidizing microorganisms in ionized water treatment was significantly lower than that of ordinary water (p < 0.05), the relative abundance of some dominant nitrifying microbial genera in the ionized water treatment was significantly higher (p < 0.05). The dominant genera may play a key role in the nitrification process. The results show that ionized water irrigation can significantly promote the nitrification of silt loam soil, especially under 100%θFC conditions, and may regulate soil nitrification by affecting some dominant nitrifying microorganisms. This study provides a theoretical basis for understanding the biological regulation mechanism of ionized water irrigation on soil nutrient transformation and for application of ionized water to field irrigation.

Crop seedling detection is an important task in the seedling stage of crops in fine agriculture. In this paper, we propose a high-precision lightweight object detection network model based on a multi-activation layer and depth-separable convolution module to detect crop seedlings, aiming to improve the accuracy of traditional artificial intelligence methods. Due to the insufficient dataset, various image enhancement methods are used in this paper. The dataset in this paper was collected from Shahe Town, Laizhou City, Yantai City, Shandong Province, China. Experimental results on this dataset show that the proposed method can effectively improve the seedling detection accuracy, with the F1 score and mAP reaching 0.95 and 0.89, respectively, which are the best values among the compared models. In order to verify the generalization performance of the model, we also conducted a validation on the maize seedling dataset, and experimental results verified the generalization performance of the model. In order to apply the proposed method to real agricultural scenarios, we encapsulated the proposed model in a Jetson logic board and built a smart hardware that can quickly detect seedlings.

Kron reduction is a general tool of network simplification for flow calculation. With a growing number of flexible loads appearing in distribution networks, traditional Kron reduction cannot be widely used in control and scheduling due to the elimination of controllable and variable load buses. Therefore, this paper proposes an improved Kron reduction based on node ordering optimization whose principles guarantee that all the boundary nodes are retained eventually after eliminating the first row and the first column in every step according to the order, thereby making it possible to take full advantage of their potential to meet different requirements in power system calculation and dispatching. The proposed method is verified via simulation models of IEEE 5-bus and 30-bus systems through illustrating the dynamic consistency of the output active power of the generator nodes and the power flow data of preserved nodes before and after reduction.

Abstract The lunar penetrating radar (LPR) onboard the Chinese Chang'e‐3 (CE‐3) mission obtained high‐resolution profile data for the continuous ejecta deposits of the Ziwei crater. Geological background suggests that the continuous ejecta deposits contain few large boulders, and the ejecta deposits were largely originated from the pre‐impact regolith. Using the top ~50 ns of radar data, we estimate the bulk density and porosity for the ejecta deposits based on hyperbolic echo patterns in the radargram that are caused by subsurface boulders. The physical properties are close to those of typical lunar regolith. Numerous subparallel and discontinuous short layers are visible in the radargram of the continuous ejecta deposits. The dielectric coefficients of the layering structures are estimated, and their permittivity is slightly larger than that of typical lunar regolith and less than that of basaltic rocks. Cratering physics together with the geological context of this area suggest that the layering structures are most likely ground gravels and/or melt‐welded breccias that were sheared due to the horizontal momentum of the impact ejecta. This interpretation is indicative of the origin of the enigmatic layering structures in regolith core samples returned by the Apollo and Luna missions. The results also highlight the importance of ejecta emplacement in shaping the structure of lunar regolith.

Gut symbiotic bacteria have a substantial impact on host physiology and ecology. However, the contribution of gut microbes to host fitness during long-term low-temperature stress is still unclear. This study examined the role of gut microbiota in host low-temperature stress resistance at molecular and biochemical levels in the oriental fruit fly Bactrocera dorsalis. The results showed that after the gut bacteria of flies were removed via antibiotic treatment, the median survival time was significantly decreased to approximately 68% of that in conventional flies following exposure to a temperature stress of 10°C. Furthermore, we found that Klebsiella michiganensis BD177 is a key symbiotic bacterium, whose recolonization in antibiotic treated (ABX) flies significantly extended the median survival time to 160% of that in the ABX control, and restored their lifespan to the level of conventional flies. Notably, the relative levels of proline and arginine metabolites were significantly downregulated by 34- and 10-fold, respectively, in ABX flies compared with those in the hemolymph of conventional flies after exposure to a temperature stress of 10°C whereas recolonization of ABX flies by K. michiganensis BD177 significantly upregulated the levels of proline and arginine by 13- and 10- fold, respectively, compared with those found in the hemolymph of ABX flies. qPCR analysis also confirmed that K. michiganensis-recolonized flies significantly stimulated the expression of transcripts from the arginine and proline metabolism pathway compared with the ABX controls, and RNAi mediated silencing of two key genes Pro-C and ASS significantly reduced the survival time of conventional flies, postexposure low-temperature stress. We show that microinjection of L-arginine and L-proline into ABX flies significantly increased their survival time following exposure to temperature stress of 10°C. Transmission electron microscopy (TEM) analysis further revealed that low-temperature stress caused severe destruction in cristae structures and thus resulted in abnormal circular shapes of mitochondria in ABX flies gut, while the recolonization of live K. michiganensis helped the ABX flies to maintain mitochondrial functionality to a normal status, which is important for the arginine and proline induction. Our results suggest that gut microbiota plays a vital role in promoting the host resistance to low-temperature stress in B. dorsalis by stimulating its arginine and proline metabolism pathway.

Summary: Netherton syndrome (NS) is a monogenic skin disease resulting from loss of function of lymphoepithelial Kazal-type-related protease inhibitor (LEKTI-1). In this study we examine if bacteria residing on the skin are influenced by the loss of LEKTI-1 and if interaction between this human gene and resident bacteria contributes to skin disease. Shotgun sequencing of the skin microbiome demonstrates that lesional skin of NS subjects is dominated by Staphylococcus aureus (S. aureus) and Staphylococcus epidermidis (S. epidermidis). Isolates of either species from NS subjects are able to induce skin inflammation and barrier damage on mice. These microbes promote skin inflammation in the setting of LEKTI-1 deficiency due to excess proteolytic activity promoted by S. aureus phenol-soluble modulin α as well as increased bacterial proteases staphopain A and B from S. aureus or EcpA from S. epidermidis. These findings demonstrate the critical need for maintaining homeostasis of host and microbial proteases to prevent a human skin disease. : Williams et al. show how an abnormal skin microbiome promotes inflammation associated with Netherton syndrome, a monogenic disorder in the human protease inhibitor SPINK5. Subjects with Netherton syndrome have excess colonization by S. aureus or S. epidermidis that then produce and promote increased protease production in the epidermis. Keywords: Netherton syndrome, S. aureus, S. epidermidis, proteases, epidermal barrier, skin inflammation, skin microbiome

Recent studies point to the evolution of drug resistance in lung cancer as being centered, at least in part, on the upregulation of various genes involved in controlling efflux or drug inactivation. Among the most important of these genes is Nuclear Factor Erythroid 2-Related Factor (NRF2), considered the master regulator of 100–200 target genes involved in cellular responses to oxidative and/or electrophilic stress. With increased focus on the development of combinatorial approaches for cancer treatment, we utilized CRISPR/Cas9 to disable the NRF2 gene in lung cancer cells by disrupting the NRF2 nuclear export signal (NES) domain; phenotypically, the protein is largely blocked from transiting into the nucleus after translation. In tissue culture, cells with this gene knockout were found to have a reduced proliferation phenotype and are more sensitive to chemotherapeutic agents, such as cisplatin and carboplatin. These observations were confirmed in xenograft mouse models wherein the homozygous knockout cells proliferate at a slower rate than the wild-type cells, even in the absence of drug treatment. Tumor growth was arrested for a period of 16 days, with a dramatic decrease in tumor volume being observed in samples receiving the combined action of CRISPR-directed gene editing and chemotherapy. Keywords: CRISPR, Cas9, gene editing, NRF2, lung cancer, chemoresistance, NSCLC

Objective: To explore the effectiveness and safety of cardiac rehabilitation qigong exercise in stable coronary artery disease (CAD) patients undergoing phase III rehabilitation. Methods: This was a randomized controlled trial. A total of 59 stable CAD patients undergoing phase III rehabilitation treated in Sports Medicine Hospital from March 2017 to September 2017 were enrolled after meeting the inclusion criteria and then divided into cardiac rehabilitation qigong exercise group (n = 30) receiving a 12-week intervention and control group (n = 29). All participants were assessed at baseline and at 12-week intervention for the primary outcome, that was treadmill test parameter and the secondary outcomes including physical fitness, body composition, bone mineral density, and cardiac ultrasound B-mode imaging. Results: There were no significant differences in baseline demographics between the two groups. After a 12-week cardiac rehabilitation qigong exercise intervention, compared with the control group, ΔVO2 (initial 1352.63 ± 340.95 vs 12 weeks 1594.57 ± 467.14) vs (initial 1363.83 ± 322.90 vs 12 weeks 1323.76 ± 318.92) (P = .003), ΔVO2/kg (initial 21.23 ± 3.56 vs 12 weeks 24.75 ± 5.11) vs (initial 21.01 ± 3.71 vs 12 weeks 20.35 ± 3.66) (P = .002), ΔMETS (initial 6.19 ± 1.12 vs 12 weeks 7.16 ± 1.60) vs (initial 6.00 ± 1.19 vs 12 weeks 5.86 ± 1.23) (P = .001), ΔVO2/HR (P = .027), ΔSV (P = .014), ΔOUES (P = .012), Δhand-grip strength (P = .002), Δflexibility (P = .001), Δbalance (P = .002), ΔT-score (P = .042), ΔBQI (P = .018). However, Δresting systolic blood pressure (P = .004) and Δresting diastolic pressure (P = .012) decreased in the cardiac rehabilitation qigong exercise group. Conclusion: Cardiac rehabilitation qigong exercise can improve cardiopulmonary aerobic capacity, physical fitness, bone mineral density in patients with stable CAD, suggesting that certain effect and safety for stable CAD patients undergoing phase III rehabilitation can be obtained. Keywords: Stable coronary artery disease, Rehabilitation, Cardiac rehabilitation qigong exercise, Cardiopulmonary aerobic capacity

Sunlight-harvesting materials require the clean integration of light-absorbing and catalytic components to be efficient. Here, authors link silicon photoelectrodes and molybdenum sulfide catalysts with defect-free gallium nitride nanowire to improve photoelectrochemical hydrogen evolution.

Objective: The aim of this study was to evaluate the effects of traditional Chinese exercises (TCEs) on stroke risk factors in patients with pre-hypertension or mild-to-moderate essential hypertension. Methods: We performed searches of seven electronic databases for studies published from their inception until July 2017. We included randomized controlled trials (RCTs) of the effects of TCEs with or without health education on stroke risk factors in patients with pre-hypertension or mild-to-moderate essential hypertension. Outcomes were independently extracted from each study by two authors and were pooled to determine risk ratios and standardized mean differences.materials-methods Results: We included 15 RCTs, involving 1272 hypertensive participants. Compared with no intervention, TCEs were associated with a clinically meaningful amelioration of stroke risk factors, including reductions in systolic blood pressure (SBP), diastolic blood pressure (DBP), fasting blood glucose, blood lipids, and waist-to-hip ratio, and improved quality of life. Compared with health education, TCEs reduced SBP but had no effect on DBP. Compared with health education alone, TCEs plus health education resulted in an amelioration of stroke risk factors. No serious adverse events were reported.results Conclusion: TCEs may be effective alongside health education in ameliorating stroke risk factors in individuals with pre-hypertension or mild-to-moderate essential hypertension. However, the current evidence is insufficient due to poor methodology and lack of adequate safety data. Further rigorously-designed RCTs are warranted.conclusion Keywords: Hypertension, Stroke risk factor, Tai Chi, Baduanjin, Yijinjin

Abstract Background Marked increases in fluconazole resistance in Candida tropicalis have been recently reported. In this study, the molecular mechanisms behind fluconazole resistance were investigated. Methods Twenty-two C. tropicalis clinical isolates, including 12 fluconazole-resistant isolates and 10 fluconazole-susceptible isolates, were collected from a tertiary care teaching hospital in Beijing between 2013 and 2017. Antifungal susceptibility testing, multilocus sequence typing, ERG11 amplification and sequencing, quantitative real-time reverse transcription-polymerase chain reaction (ERG11, UPC2, MDR1, and CDR1), and clinical data collection were performed for all C. tropicalis isolates. Results Multilocus sequence typing revealed that the 10 fluconazole-susceptible isolates and 12 fluconazole-resistant isolates were divided into nine and seven diploid sequence types, respectively. Of the 12 patients with fluconazole-resistant isolates, six had been previously exposed to azole and four had a fatal outcome. Y132F and S154F amino acid substitutions in Erg11p were found in all fluconazole-resistant isolates except one. MDR1 gene overexpression was identified in fluconazole-resistant isolates. In particular, seven high-level fluconazole resistant isolates (minimum inhibitory concentration ≥ 128 mg/L) and three pan-azole resistant isolates were identified. CDR1, ERG11, and UPC2 gene expression levels in fluconazole-resistant isolates were not significantly different from the control isolates (P = 0.262, P = 0.598, P = 0.114, respectively). Conclusions This study provides evidence that the combination of MDR1 gene overexpression and ERG11 missense mutations is responsible for high-level fluconazole resistance and pan-azole resistance in C. tropicalis clinical isolates. To the best of our knowledge, this is the first study investigating the relationship between MDR1 gene overexpression and increased fluconazole resistance.

Agarwood is a highly valuable fragrant wood of Aquilaria spp. (Thymelaeaceae) which has been widely utilized in traditional medicine, religious rites, and cultural activities. This study summarizes a review on the identification of Aquilaria cultivars, volatile and non-volatile phytochemicals, pharmacological uses, and agarwood grading system to determine its quality, and different agarwood induction methods. Due to the highly demanding and depleted natural resources, the research on agarwood is still insufficient, and it has broad research and development prospects in many industries. However, due to the significant scientific nature of agarwood application, developing high-quality products and drugs from agarwood have become highly important, while no one has discussed in detail the phytochemicals uses and provided a summary until now. The main phytochemicals of agarwood include terpenoids, dominated by sesquiterpenes. For centuries, terpenoids have been used in traditional Chinese medicine and have been shown to possess various pharmacological properties, including bacteriostatic, antibacterial, sedation, analgesia, anti-inflammation, anti-asthmatic, hypoglycemic, antidepressant, and many others. Alongside biological activity screening, phytochemical advances and pharmacological research have also made certain progress. Therefore, this review discusses the research progress of agarwood in recent years and provides a reference basis for further study of Aquilaria plants and agarwood.

Secure the cooperative transmission is studied for an automatic repeat request (ARQ)-based wireless network against a jamming-aided eavesdropper. The eavesdropper utilizes the jamming attack to assist its eavesdropping by forcing the legitimate transmitter to retransmit its confidential messages under poor wiretap channel quality. Then, utilizing these retransmission opportunities, the eavesdropper will eavesdrop the confidential message under good wiretap channel quality. To address the threat from the jamming-aided eavesdropper in the ARQ-based wireless network, we propose an adaptive cooperative relaying and jamming secure transmission scheme to protect the confidential messages. In the proposed scheme, the legitimate receiver adopts the energy detection method to detect the jamming-aided eavesdropper's action and a cooperative node will aid the secure transmission through cooperative relaying under jamming attack or cooperative jamming under eavesdropping attack. By taking into account of the detection errors, we investigate the transmission outage probability and the secrecy outage probability, and derive their closed-form expressions for both one retransmission and multiple retransmissions scenarios. Numerical results are presented to verify the derived analytical results and demonstrate the performance superiority of the proposed scheme in terms of the secrecy outage probability.

Atractylodes macrocephala is one of the most commonly used herbs in China, which is famous for its high medicinal value. In this study, we analyzed and characterized the complete plastome sequence of A. macrocephala. Sequence analysis indicated that the entire genome is 153,265 bp in length, consisting of a large single-copy (LSC, 84,311 bp) and a small single-copy (SSC, 18,674 bp) region separated by a pair of inverted repeat (IR) regions of 25,140 bp for each. The genome contains 107 unique genes, including 80 different protein-coding genes, 23 tRNA genes, and 4 rRNA genes. The overall GC content of the genome is 37.7%. The phylogenetic analysis revealed a monophyletic Atractylodes and Cardueae. This research reports the complete plastome genome of Atractylodes macrocephala, which provides a better understanding of this important herb.

Bactrocera minax (Enderlein) (Diptera: Tephritidae) is an oligophagous insect pest that damages citrus fruit, especially in China. Due to larvae living within a highly septic environment, a wide variety of microorganisms exist in the larval gut of B. minax. However, a systematic study of the intestinal microbiota of this harmful insect pest is still lacking. Here, we comprehensively investigated the larval gut microbiota of B. minax in two field populations from Zigui (developed in orange) and Danjiangkou (developed in mandarin orange). We observed a dominance of Proteobacteria and Firmicutes in these bacterial communities, and Enterobacteriaceae was the predominant family throughout the larval stage. However, most of the identified fungal sequences were annotated as being from either Ascomycota or Basidiomycota phyla. Although there was a difference in the structure of the microbial communities between the two populations, the dynamic change patterns of most of the members of the microbiota were similar across the lifespan of larvae in both populations. The relative abundances of the Acetobacteraceae, Leuconostocaceae, and Lactobacillaceae gut bacteria as well as the Pichiaceae, Sebacinaceae, and Amanitaceae fungi increased throughout development, and these microorganisms stably resided in the larval gut. Furthermore, the dynamic changes of the functions of gut bacterial communities were inferred, and there was a significant increase in carbohydrate metabolism across the lifespan of larvae in both groups. Spearman correlation analysis showed that Acetobacteraceae, Lactobacillaceae, and Leuconostocaceae displayed a positive correlation with fructose and mannose metabolism, an important pathway of carbohydrate metabolism, highlighting the potential roles of these prevalent microbial communities in host biology.

Aristolochic acid nephropathy (AAN), as a rapidly progressive interstitial nephropathy due to excessive ingestion of aristolochia herbal medications, has recently raised considerable concerns among clinicians and researchers as its underlying pathogenic mechanisms are largely unclear. In the current study, we identified NLRP3 inflammasome activation as a novel pathological mechanism of AAN. We found that NLRP3 inflammasome was aberrantly activated both in vivo and in vitro after AA exposure. Blockade of IL-1β and NLRP3 inflammasome activation by IL-1Ra significantly attenuated renal tubular injury and function loss in AA-induced nephropathy. Moreover, NLRP3 or Caspase-1 deficiency protected against renal injury in the mouse model of acute AAN, suggesting that the NLRP3 signaling pathway was probably involved in the pathogenesis of AAN. We also found that administration of IL-22 could markedly attenuate renal tubular injury in AAN. Notably, IL-22 intervention significantly alleviated renal fibrosis and dysfunction in AA-induced nephropathy. Furthermore, IL-22 largely inhibited renal activation of NLRP3 inflammasome in AA-induced nephropathy. These results indicated that IL-22 ameliorated renal tubular injury in AAN through suppression of NLRP3 inflammasome activation. In summary, this study identified renal activation of NLRP3 inflammasome as a novel mechanism underlying the pathogenesis of AAN, thus providing a potential therapeutic strategy for AAN based on suppression of NLRP3 inflammasome activation.

Remote sensing images contain complex backgrounds and multi-scale objects, which pose a challenging task for scene classification. The performance is highly dependent on the capacity of the scene representation as well as the discriminability of the classifier. Although multiple models possess better properties than a single model on these aspects, the fusion strategy for these models is a key component to maximize the final accuracy. In this paper, we construct a novel dual-model architecture with a grouping-attention-fusion strategy to improve the performance of scene classification. Specifically, the model employs two different convolutional neural networks (CNNs) for feature extraction, where the grouping-attention-fusion strategy is used to fuse the features of the CNNs in a fine and multi-scale manner. In this way, the resultant feature representation of the scene is enhanced. Moreover, to address the issue of similar appearances between different scenes, we develop a loss function which encourages small intra-class diversities and large inter-class distances. Extensive experiments are conducted on four scene classification datasets include the UCM land-use dataset, the WHU-RS19 dataset, the AID dataset, and the OPTIMAL-31 dataset. The experimental results demonstrate the superiority of the proposed method in comparison with the state-of-the-arts.

Urban 3D surface reflectance is a critical parameter for the modeling of surface biophysical processes. It is of great significance to enhance the accuracy of reflectance in urban areas. Based on the urban solar radiative transfer (USRT) model, this study presents a methodology for estimating urban reflectance using the sky view factor (SVF) derived from airborne LiDAR data. Then, the USRT model was used to retrieve urban 3D surface reflectance from Landsat 8 data over the typical area of Beijing. The reflectance from USRT model was compared with the estimated value obtained from the model without considering the impact of morphological characteristics of the urban underlying surface (flat model). The results showed that the urban sample reflectance estimated by the USRT model was close to the sample reflectance of the suburban underlying surface which was less affected by morphological characteristics. The research summaries are as follows: (1) The definite physical meaning is presented in the USRT model, and can be applied to estimate the physical parameters of the urban underlying surface. (2) The reflectance from the USRT model is slightly larger than the reflectance derived from the flat model, which indicates that the accuracy of urban 3D surface reflectance is improved by the USRT model. (3) The effects of the SVF and building reflectance are different. The SVF presents a strong sensitivity to the estimation of the urban 3D surface reflectance, and the variations of building reflectance setting have little impact on urban reflectance, which is characterized by low sensitivity. Generally, the methodology of estimating urban reflectance proposed in this study can better clarify the impact mechanism of urban geometry on the radiative transfer processes and further promote the application and development of urban quantitative remote sensing.

As a green and preferred technology for energy crisis and environmental issues, continuous research on photocatalysis and gas sensing has come forth at an explosive rate. Thus far, promising synthetic methods have enabled various designs and preparations of semiconductor-based nanostructure which have shown superior activity. This review summarized various synthetic routines toward carbon sphere template derived hollow nanostructures and their successful attempts in synthesize doping, solid solution, heterostructure, and surface modified nanostructures for heterogeneous photocatalysis and gas sensing. Moreover, the challenges and future prospects are briefly discussed. It is eagerly anticipated that this review may broaden the view and in-depth understanding of carbon sphere template derived hollow nanostructures while expected to have further progresses in heterogeneous photocatalysis, gas sensing and other related fields which will make great contributions to their application.

Soil water repellency (SWR) is a widespread property in soils around the world. It influences soil water movement and causes unstable flow. The mechanics of finger flow occurrence in water-repellent soils are not clearly understood. Slab chamber infiltration experiments consisting of increasing SWR persistence in clay and sandy loam soils were conducted to compare wetting front advancement and mechanics of finger flow development. The temporal variation curves of cumulative infiltration (CI) decreased with an increase in SWR persistence. For wettable soils, the wetting front advanced regularly with time, but for water-repellent soils, it became unstable and finger flow occurred as the SWR persistence increased. Water movement in soils with higher SWR persistence tended to be more unstable. Water repellency contributed to finger flow development, especially for sandy loam. There were strong correlations among CI, finger length, and the cumulative wetting area. In the strongly, severely, and extremely water-repellent sandy loam soils, the power function relationship fit better than the linear function. The average soil water content decreased with a higher SWR persistence, which meant that less water was available in the profile. Finger flow development was related to the more severe water-repellent conditions and tended to be more easily formed in the water-repellent sandy loam soils than the clay loam soils due to the faster infiltration rate.

BackgroundAntibody drug conjugate (ADC) showed potent therapeutic efficacy in several types of cancers. The role of autophagy in antitumor effects of ADC remains unclear.MethodsIn this study, the ADC, Rituximab-monomethyl auristatin E (MMAE) with a Valine–Citrulline cleavable linker, was designed to investigate its therapeutic efficacy against non-Hodgkin lymphoma (NHL) as well as the underlying mechanisms. Methylthiazolyldiphenyl-tetrazolium bromide (MTT) was used to detect growth inhibition in B-cell lymphoma cell lines, Ramos and Daudi cells, which were treated by Rituximab-MMAE alone or combined with autophagy conditioner. Apoptosis was detected by flow cytometry and immunohistochemistry, and apoptosis inhibitor was employed to discover the relationship between autophagy and apoptosis during the Rituximab-MMAE treatment. Autophagy was determined by three standard techniques which included confocal microscope, transmission electron microscope, and western blots. Ramos xenograft tumors in BALB/c nude mice were established to investigate the antitumor effect of combination use of Rituximab-MMAE and autophagy conditioner in B-NHL therapy.ResultsOur results showed that Rituximab-MMAE elicited caspase-3-dependent apoptosis in NHL cells and exhibited potent therapeutic efficacy in vivo. Autophagy, which was characterized by upregulated light chain 3-II expression, and accumulation of autophagosomes, was triggered during the Rituximab-MMAE treatment. Meanwhile, inactivation of Akt/mTOR pathway was shown to be involved in the autophagy triggered by Rituximab-MMAE, indicating a probable mechanism of the ADC-initiated autophagy. Importantly, inhibition of autophagy by chloroquine suppressed the Rituximab-MMAE-induced apoptosis, while activating autophagy by rapamycin significantly enhanced the therapeutic effect of Rituximab-MMAE both in vitro and in vivo.ConclusionOur data elucidated the critical relationship between autophagy and apoptosis in Rituximab-MMAE-based therapy and highlighted the potential approach for NHL therapy by combined administration of the ADC and autophagy activator.

Autoimmune hepatitis (AIH) is a progressive inflammatory disorders of unknown etiology, characterized by immune-mediated destruction of hepatocytes and massive production of cytokines. Interleukin-1β is a pleiotropic proinflammatory cytokine and well known to be critical in a variety of autoimmune diseases. However, the role of interleukin-1β (IL-1β) in AIH is still indistinct. Here, we first investigated the significance of NOD-like receptor protein 3 (NLRP3) inflammasome-dependent IL-1β in the pathogenesis of AIH with a murine model of immune-mediated hepatitis induced by Concanavalin A (ConA). In ConA-treated mice, pathogenic elevated NLRP3, Cleaved caspase-1 and IL-1β levels, as well as an inflammatory cell death known as pyroptosis predominantly occurred in the livers. Strikingly, NLRP3−/− and caspase-1−/− mice were broadly protected from hepatitis as determined by decreased histological liver injury, serum aminotransferase (ALT)/aspartate transaminase levels, and pyroptosis. In vivo intervention with recombinant human interleukin-1 receptor antagonist (rhIL-1Ra) strongly suppressed ConA-induced hepatitis by decreasing tumor necrosis factor-alpha (TNF-α) and interleukin-17 (IL-17) secretion, and inflammatory cell infiltration into livers. Additionally, rhIL-1Ra-pretreated mice developed significantly reduced NLRP3 inflammasome activation and reactive oxygen species (ROS) generation. Scavenging of ROS by N-acetyl-cysteine also attenuated NLRP3 inflammasome activation and liver inflammation, indicating that the essential role of ROS in mediating NLRP3 inflammasome activation in ConA-induced hepatitis. In conclusion, our results demonstrated that NLRP3 inflammasome-dependent IL-1β production was crucial in the pathogenesis of ConA-induced hepatitis, which shed light on the development of promising therapeutic strategies for AIH by blocking NLRP3 inflammasome and IL-1β.

Finger flow in water-repellent (WR) soils significantly influences the transport of water and solutes in the soil, but the mechanics of finger flow occurrence in layered WR soils is not clear. Soil chamber infiltration experiments with a total of 20 treatments, including five different WR levels with four layer combinations, i.e., clay or sandy loam overlying sand or heavy gravel, were conducted to reveal the mechanics of finger flow occurrence in layered WR soils. The variations of the finger flow dynamics and infiltration parameters were investigated. The results showed: (i) the temporal variations of cumulative infiltration (CI) decreased with the increase of the WR level so that CI was generally larger when the top layer was sandy loam rather than clay loam and therefore the top layer soil texture controlled CI more than the sublayer; (ii) for the wettable treatments, finger flow was clearly and uniformly generated in layered soils with a sublayer of heavy gravel rather than sand, but for WR layered treatments, fingers developed irregularly with the WR levels and finger length, width, and velocity varied with the WR levels; (iii) there were good power function or linear correlations between CI and cumulative wetting area, and between CI and finger length; and (iv) water content in the top layer was higher than in the sublayer and generally decreased with the increase of WR level. Finger flow development in layered WR soils was generally irregular and showed a large degree of complexity.

Comparisons of aerosol composition and sources in different cities or regions are rather limited, yet important for an in-depth understanding of the spatial diversity of aerosol pollution in China. In this study, the data originating from 25 different winter aerosol mass spectrometer (AMS)/aerosol chemical speciation monitor (ACSM) studies were used to provide spatial coverage of the Beijing-Tianjin-Hebei (BTH), Guanzhong (GZ), Yangtze River Delta (YRD), and Pearl River Delta (PRD) regions. The spatial distribution and diurnal variations in aerosol composition and organic sources were analyzed to investigate the aerosol characteristics in the four regions. It was found that there were differences in the compositions of non-refractory particulate matter across the regions, e.g., more sulfate in the PRD versus more nitrate in the YRD, as well as in the organic sources, e.g., more coal combustion in BTH versus more biomass burning in GZ. The characteristics of the composition of NR-PM are similar when the campaigns were classified according to the winter of different years or the cities of different regions. The diurnal variation of the PRD-sulfate indicated its regional nature, whereas the organics from burning sources in two regions of northern China exhibited local characteristics. Based on these findings, we suggest that strict control policies for coal combustion and biomass burning emissions should be enforced in the BTH and GZ regions, respectively.

Skeletal muscle atrophy induced by aging (sarcopenia), inactivity, and prolonged fasting states (starvation) is predominantly restricted to glycolytic type II muscle fibers and typical spares oxidative type I fibers. However, the mechanisms accounting for muscle fiber-type specificity of atrophy have remained enigmatic. In the current study, although the Fyn tyrosine kinase activated the mTORC1 signaling complex, it also induced marked atrophy of glycolytic fibers with relatively less effect on oxidative muscle fibers. This was due to inhibition of macroautophagy via an mTORC1-independent but STAT3-dependent reduction in Vps34 protein levels and decreased Vps34/p150/Beclin1/Atg14 complex 1. Physiologically, in the fed state endogenous Fyn kinase activity was increased in glycolytic but not oxidative skeletal muscle. In parallel, Y705-STAT3 phosphorylation increased with decreased Vps34 protein levels. Moreover, fed/starved regulation of Y705-STAT3 phosphorylation and Vps34 protein levels was prevented in skeletal muscle of Fyn null mice. These data demonstrate a Fyn/STAT3/Vps34 pathway that is responsible for fiber-type-specific regulation of macroautophagy and skeletal muscle atrophy.