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Plasmonic sensors have attracted intensive attention due to their high sensitivity. However, due to intrinsic metallic loss, plasmonic sensors usually have a large full width at half maximum (FWHM) that limits the wavelength resolution. In this paper, we numerically investigate and propose a dielectric grating-assisted plasmonic device, leveraging the bound states in the continuum (BIC) effect to suppress the FWHM of the resonance. We initiate quasi-SP-BIC modes at 1559 nm and 1905 nm wavelengths by slightly tilting the incident angle at 2° to break the symmetry, featuring a narrow linewidth of 1.8 nm and 0.18 nm at these two wavelengths, respectively. Refractive index sensing has also been investigated, showing high sensitivity of 938 nm/RIU and figure of merit (FOM) of 521/RIU at 1559 nm and even higher sensitivity of 1264 nm/RIU and FOM of 7022/RIU at 1905 nm.

Background: Lower socioeconomic status (SES) is associated with symptomatic severity, comorbidity, and functional impairment in adults with bipolar disorder (BD). Little is known about clinical correlates of SES in adolescents with BD. Methods: Participants included 195 adolescents, 13–20 years old, with BD type I, II or not otherwise specified (NOS). Diagnoses were determined by standardized semi-structured interviews. Based on the Hollingshead scale, participants were divided into “low” (SES 1–3) and the “high” (SES 4–5) SES groups. Demographic and clinical correlates of SES were evaluated in univariate analyses; significant variables were evaluated in a logistic regression model. Results: Compared to participants in the high SES group (n = 150), participants in the low SES group (n = 45) were significantly younger, less likely to be of Caucasian race and living with natural parents. In the logistic regression model, controlling for age and race, the low SES group had higher risk of police contact or arrest (OR = 2.41, 95% CI:1.14–5.11, p = 0.022), less treatment with stimulants(OR = 0.20 95% CI: 0.06–0.67, p = 0.009), and more post-traumatic stress disorder (PTSD) (OR = 4.08, 95% CI:1.33–12.46, p = 0.014) compared to the high SES group. In sensitivity analyses that further controlled for intact family, the finding of higher rates of police contact or arrest was no longer significant. Limitations: Cross-sectional design; higher-skewed SES sample. Conclusions: Lower SES in adolescent BD is associated with higher legal risk, increased PTSD, and under-treatment of attention-deficit/hyperactivity disorder (ADHD). Future studies are needed to evaluate the inter-relationships of these correlates, using prospective designs that can evaluate the direction of these associations. Further studies incorporating neurobiological markers are also needed to explore mechanisms underlying SES-related differences in BD.

Abstract. Introduction:. Comprehensive mRNA sequencing is a powerful tool for conducting unbiased, quantitative differential gene expression analysis. However, the reliability of these data is contingent on the extraction of high-quality RNA from samples. Preserving RNA integrity during extraction can be problematic, especially in tissues such as skin with dense, connective matrices and elevated ribonuclease expression. This is a major barrier to understanding the influences of altered gene expression in many preclinical pain models and clinical pain disorders where skin is the site of tissue injury. Objective:. This study developed and evaluated extraction protocols for skin and other tissues to maximize recovery of high-integrity RNA needed for quantitative mRNA sequencing. Methods:. Rodent and human tissue samples underwent one of the several different protocols that combined either RNA-stabilizing solution or snap-freezing with bead milling or cryosectioning. Indices of RNA integrity and purity were assessed for all samples. Results:. Extraction of high-integrity RNA is highly dependent on the methods used. Bead-milling skin collected in RNA-stabilizing solution resulted in extensive RNA degradation. Snap-freezing in liquid nitrogen was required for skin and highly preferable for other tissues. Skin also required cryosectioning to achieve effective penetration of RNA-stabilizing solution to preserve RNA integrity, whereas bead milling could be used instead with other tissues. Each method was reproducible across multiple experimenters. Electrophoretic anomalies that skewed RNA integrity value assignment required manual correction and often resulted in score reduction. Conclusion:. To achieve the potential of quantitative differential gene expression analysis requires verification of tissue-dependent extraction methods that yield high-integrity RNA.

China reported a major outbreak of a novel coronavirus, SARS-CoV2, from mid-January till mid-March 2020. We review the epidemic virus growth and decline curves in China using a phenomenological logistic growth model to summarize the outbreak dynamics using three parameters that characterize the epidemic's timing, rate and peak. During the initial phase, the number of virus cases doubled every 2.7 days (range 2.2-4.4 across provinces). The rate of increase in the number of reported cases peaked approximately 10 days after suppression measures were started on 23-25 January 2020. The peak in the number of reported sick cases occurred on average 18 days after the start of suppression measures. From the time of starting measures till the peak, the number of cases increased by a factor 39 in the province Hubei, and by a factor 9.5 for all of China (range: 6.2-20.4 in the other provinces). Complete suppression took up to 2 months (range: 23-57d.), during which period severe restrictions, social distancing measures, testing and isolation of cases were in place. The suppression of the disease in China has been successful, demonstrating that suppression is a viable strategy to contain SARS-CoV2.

Cell wall biosynthesis plays essential roles in cell division and expansion and thus is fundamental to plant growth and development. In this work, we show that an Arabidopsis mutant dpr3, isolated by a forward genetic screen, displays embryo defects and short, swelling primary root with the failure of maintenance of root apical meristem reminiscent to several cell wall–deficient mutants. Map-based cloning identified dpr3 is a mutant allele of RIBOSE PHOSPHATE ISOMERSASE 1 (RPI1), an enzyme involved in cellulose synthesis. Cellulose content in the mutant was dramatically decreased. Moreover, dpr3 (rpi1 from hereon) caused aberrant auxin distribution, as well as defective accumulation of root master regulators PLETHORA (PLT1 and PLT2) and misexpression of auxin response factor 5 (MONOPTEROS, MP). The abnormal auxin distribution is likely due to the reduced accumulation of auxin efflux transporters PIN-FORMED (PIN1 and PIN3). Surprisingly, we found that the orientation of actin microfilaments was severely altered in rpi1 root cells, whereas the cortical microtubules stay normal. Our study provides evidence that the defects in cellulose synthesis in rpi1 affect polar auxin transport possibly connected with altered F-actin organization, which is critically important for vesicle trafficking, thus exerting effects on auxin distribution, signaling, and auxin-mediated plant development.

Abstract Background Hypoxemia is a major complication in obese patients undergoing gastrointestinal endoscopy under intravenous anesthesia or sedation due to altered airway anatomy. We design this randomized controlled trial (RCT) to compare efficacy and safety of the Wei nasal jet tube (WNJT) and nasal prongs for supplement oxygen during gastroscopy with intravenous propofol anesthesia in obese patients. Methods The study will be a single-center, prospective RCT. A total of 308 obese patients will be recruited and randomly assigned to receive either the WNJT (group A) or nasal prongs (group B). During gastroscopy with intravenous propofol anesthesia, 5 L/min of oxygen will be delivered through the jet port of the WNJT in the group A and via the nasal prongs in the group B. The primary outcome is the incidence of hypoxemia and severe hypoxemia. The secondary outcomes are adverse events during the gastroscopy, postoperative complications, and satisfaction of the anesthetist, physician, and patient. Discussion This RCT aims to clarify whether the WNJT can result in reduced incidences of hypoxemia and complications and provide improved satisfaction to the anesthetist, physician, and patient. Thus, it can be determined if the WNJT is a useful tool for supplement oxygen in obese patients undergoing gastroscopy with intravenous propofol anesthesia. The results will provide the evidence for anesthesiologists to make a decision regarding the choice of supplementary oxygen methods in this condition. Trial registration Chinese Clinical Trial, ChiCTR-IOR-17013089. Registered on 23 October 2017.

In the computer vision field, understanding human dynamics is not only a great challenge but also very meaningful work, which plays an indispensable role in public safety. Despite the complexity of human dynamics, physicists have found that pedestrian motion in a crowd is governed by some internal rules, which can be formulated as a motion model, and an effective model is of great importance for understanding and reconstructing human dynamics in various scenes. In this paper, we revisit the related research in social psychology and propose a two-part motion model based on the shortest path principle. One part of the model seeks the origin and destination of a pedestrian, and the other part generates the movement path of the pedestrian. With the proposed motion model, we simulated the movement behavior of pedestrians and classified them into various patterns. We next reconstructed the crowd motions in a real-world scene. In addition, to evaluate the effectiveness of the model in crowd motion simulations, we created a new indicator to quantitatively measure the correlation between two groups of crowd motion trajectories. The experimental results show that our motion model outperformed the state-of-the-art model in the above applications.

Considering the vital role of college teachers' teaching engagement in guaranteeing the quality of higher education, this study aimed to reveal the behavioral mechanism of Chinese college teachers' teaching engagement through qualitative study based on the grounded theory. It is found that the behavior of Chinese college teachers' teaching engagement is the result of the corresponding intention, which is affected by the three factors of the teaching engagement values, the perceived expectations from the significant others, and the professional knowledge and ability to teach through different ways of interaction. According to the findings, the teaching engagement of college teachers needs to be promoted from three paths: improve teachers' satisfaction of values demands in teaching engagement, reform policy regimes to create a cultural atmosphere that emphasizes teaching engagement, and enhance teachers' professional knowledge and ability of teaching.

We propose a photonic array of resonant circular dielectric waveguides with subwavelength grating that can be designed as a robust and sensitive topological chemical sensor. The device can detect trace amounts of a given chemical species through photonic edge modes that are impervious to most sources of disorder. We perform a simulation in the midinfrared that accounts for the absorption loss introduced by chemical molecules in contact with a strongly coupled photonic lattice of resonators. Due to the topological nature of the device, its chemical sensitivity scales linearly with the system size and can reach parts-per-billion range at the millimeter scale. Our findings suggest that topological chemical sensors could empower the development of novel on-chip integrated photonic sensing technologies.

Blockade of the programmed cell death 1 (PD-1)/programmed cell death-ligand 1 (PD-L1) interaction is currently the focus in the field of cancer immunotherapy, and so far, several monoclonal antibodies (mAbs) have achieved encouraging outcomes in cancer treatment. Despite this achievement, mAbs-based therapies are struggling with limitations including poor tissue and tumor penetration, long half-life time, poor oral bioavailability, and expensive production costs, which prompted a shift towards the development of the small-molecule inhibitors of PD-1/PD-L1 pathways. Even though many small-molecule inhibitors targeting PD-1/PD-L1 interaction have been reported, their development lags behind the corresponding mAb, partly due to the challenges of developing drug-like small molecules. Herein, we report the discovery of a series of novel inhibitors targeting PD-1/PD-L1 interaction via structural simplification strategy by using BMS-1058 as a starting point. Among them, compound A9 stands out as the most promising candidate with excellent PD-L1 inhibitory activity (IC50 = 0.93 nM, LE = 0.43) and high binding affinity to hPD-L1 (KD = 3.64 nM, LE = 0.40). Furthermore, A9 can significantly promote the production of IFN-γ in a dose-dependent manner by rescuing PD-L1 mediated T-cell inhibition in Hep3B/OS-8/hPD-L1 and CD3-positive T cells co-culture assay. Taken together, these results suggest that A9 is a promising inhibitor of PD-1/PD-L1 interaction and is worthy for further study.

A secure scheme based on inter-relay interference cancellation was proposed to solve untrusted relay eavesdropping problem in successive relaying systems.First inter-relay interference between trusted relay and untrusted relay was exploited as artificial noise to degrade untrusted relay’s eavesdropping condition,then interference iterative cancellation was adapted to improve signal quality at legitimate user by making use of correlation between signals in time domain.Based on this,a quick power allocation algorithm aiming to minimize untrusted relay’s sum rate was proposed.Analysis and simulation results show that the proposed scheme suppress untrusted relay’s eavesdropping without loss of communication efficiency,a promotion of at least 2 bit·s-1·Hz-1in secrecy rate can be observed.

Abstract While myelin deficit of the central nervous system leads to several severe diseases, the definitive diagnostic means are lacking. We proposed and performed terahertz time-domain spectroscopy (THz-TDS) combined with chemometric techniques to discriminate and evaluate the severity of myelin deficit in mouse and rhesus monkey brains. The THz refractive index and absorption coefficient of paraffin-embedded brain tissues from both normal and mutant dysmyelinating mice are shown. Principal component analysis of time-domain THz signal (PCA-tdTHz) and absorption-refractive index relation of THz spectrum identified myelin deficit without exogenous labeling or any pretreatment. Further, with the established PCA-tdTHz, we evaluated the severity of myelin deficit lesions in rhesus monkey brain induced by experimental autoimmune encephalomyelitis, which is the most-studied animal model of multiple sclerosis. The results well matched the pathological analysis, indicating that PCA-tdTHz is a quick, powerful, evolving tool for identification and evaluation myelin deficit in preclinical animals and potentially in para-clinical human biopsy.

Bulk silicon possesses no second-order susceptibility (χ(2)), inhibiting second-order nonlinear processes in the emerging silicon photonic platform. Here, we propose a method to overcome this limitation by enabling a third-order (χ(3)) nonlinear mixing scheme between optical waves and an externally applied static electric field inside a silicon waveguide. We show in theory that facilitated by a modal phase-matching scheme efficient second-harmonic generation can be realized under an applied voltage of 65 V, giving rise to an equivalent χ(2) = 4.7 pm/V. We also show that unlike the classical second-harmonic generation, the wavelengths of phase-matched pump and second-harmonic waves are pump-power dependent due to the χ (3) nature of this process.

Enormous enormous subgaleal hematoma with epidural extension, accompanying occipital bone's absorption is an uncommon complication of hemophilia A. A 30 years old man presenting scalp lump was found enormous enormous subgaleal hematoma with epidural extension, accompanying occipital bone's destruction by computed tomography (CT) scan, and laboratory examination indicated that the concentration of his factor VII was 2% of normal level. Further inquiring his medical history found that this patient has been suffering from hemophilia A diagnosed when he was 6 years old. He recovered well after receiving the therapertic methods of factor VII replacement and surgical decompression. This case tells us that patient suffering from enormous enormous subgaleal hematoma with epidural extension, accompanying occipital bone's destruction and hemophilia A could recover well as long as he is well treated. Keywords: Neurosurgery, Factor VII, Enormous subgaleal hematoma with epidural extension, occipital bone's destruction, Hemophilia A

In recent years, developing students' knowledge integration of specific topics through effective teaching methods has been a concern in science education. This study explored an alternative teaching method, which aims to help lower secondary school students develop their knowledge structure about buoyancy towards a logically integrated conceptual framework through enhancing their understanding of the nature of buoyancy. Specifically, this study constructed a conceptual framework on buoyancy and a set of matching test questions to map out the three levels of knowledge integration of students, and designed and implemented a teaching experiment of promoting students' understanding of the nature of buoyancy. Through quantitative and qualitative analysis, it was found that the knowledge integration development of the treatment group was significantly better than that of the control group. The results indicated that enhancing students' understanding of the nature of buoyancy can effectively improve their knowledge integration of buoyancy. Accordingly, improving knowledge integration through enhancing students' understanding of the nature of the specific scientific concept may be a new direction for future research.

Humans show sex differences related to alcohol use disorders (AUD). Animal model research has the potential to provide important insight into how sex differences affect alcohol consumption, particularly because female animals frequently drink more than males. In previous work, inbred strains of the selectively bred alcohol-preferring (P) and non-preferring (NP) rat lines revealed a highly significant quantitative trait locus (QTL) on rat chromosome 4, with a logarithm of the odds score of 9.2 for alcohol consumption. Recently, interval-specific congenic strains (ISCS) were developed by backcrossing the congenic P.NP line to inbred P (iP) rats to further refine the chromosome 4 QTL region. Two ISCS sub-strains, ISCS-A and ISCS-B, were obtained with a narrowed QTL, where the smallest region of overlap consisted of 8.9 Mb in ISCS-B. Interestingly, we found that females from both ISCS lines consumed significantly less alcohol than female iP controls (p < 0.05), while no differences in alcohol consumption were observed between male ISCS and iP controls. RNA-sequencing was performed on the nucleus accumbens of alcohol-naïve female ISCS-B and iP rats, which revealed differentially expressed genes (DEG) with greater than 2-fold change and that were functionally relevant to behavior. These DEGs included down-regulation of Oxt, Asb4, Gabre, Gabrq, Chat, Slc5a7, Slc18a8, Slc10a4, and Ngfr, and up-regulation of Ttr, Msln, Mpzl2, Wnt6, Slc17a7, Aldh1a2, and Gstm2. Pathway analysis identified significant alterations in gene networks controlling nervous system development and function, as well as cell signaling, GABA and serotonin receptor signaling and G-protein coupled receptor signaling. In addition, β-estradiol was identified as the most significant upstream regulator. The expression levels of estrogen-responsive genes that mapped to the QTL interval and have been previously associated with alcohol consumption were measured using RT-qPCR. We found that expression of the Adcyap1r1 gene, encoding the pituitary adenylate cyclase-activating polypeptide type 1 (PAC1) receptor, was upregulated in female ISCS-B compared to female iP controls, while no differences were exhibited in males. In addition, sequence variants in the Adcyap1r1 promoter region showed a differential response to estrogen stimulation in vitro. These findings demonstrate that rat chromosome 4 QTL contains genetic variants that respond to estrogen and are associated with female alcohol consumption.

Defect-engineered photonic crystal (PC) microcavities were fabricated by UV photolithography and their corresponding sensitivities to biomarkers in patient plasma samples were compared for different resonant microcavity characteristics of quality factor Q and biomarker fill fraction. Three different biomarkers in plasma from pancreatic cancer patients were experimentally detected by conventional L13 defect-engineered microcavities without nanoholes and higher sensitivity L13 PC microcavities with nanoholes. 8.8 femto-molar (0.334 pg/mL) concentration of pancreatic cancer biomarker in patient plasma samples was experimentally detected which are 50 times dilution than ELISA in a PC microcavity with high quality factor and high analyte fill fraction.

Many phylogenetic questions in the Ciconiiformes remain unresolved and complete mitogenome data are urgently needed for further molecular investigation. In this work, we determined the complete mitogenome sequence of the little egret (Egretta garzetta). The genome was 17,361 bp in length and the gene organization was typical of other avian mtDNA. In protein-coding genes (PCGs), a C insertion was found in ND3, and CO III and ND4 terminated with incomplete stop codons (T). tRNA-Val and tRNA-Ser (AGY) were unable to fold into canonical cloverleaf secondary structures because they had lost the DHU arms. Long repetitive sequences consisting of five types of tandem repeats were found at the 3′ end of Domain III in the control region. A phylogenetic analysis of 11 species of Ciconiiformes was done using complete mitogenome data and 12 PCGs. The tree topologies obtained with these two strategies were identical, which strongly confirmed the monophyly of Ardeidae, Threskiorothidae and Ciconiidae. The phylogenetic analysis also revealed that Egretta was more closely related to Ardea than to Nycticorax in the Ardeidae, and Platalea was more closely related to Threskiornis than to Nipponia in the Threskiornithidae. These findings contribute to our understanding of the phylogenetic relationships of Ciconiiformes based on complete mitogenome data.

Phrenic nerve transfer is a major dynamic treatment used to repair brachial plexus root avulsion. We analyzed 72 relevant articles on phrenic nerve transfer to repair injured brachial plexus that were indexed by Science Citation Index. The keywords searched were brachial plexus injury, phrenic nerve, repair, surgery, protection, nerve transfer, and nerve graft. In addition, we performed neurophysiological analysis of the preoperative condition and prognosis of 10 patients undergoing ipsilateral phrenic nerve transfer to the musculocutaneous nerve in our hospital from 2008 to 201 3 and observed the electromyograms of the biceps brachii and motor conduction function of the musculocutaneous nerve. Clinically, approximately 28% of patients had brachial plexus injury combined with phrenic nerve injury, and injured phrenic nerve cannot be used as a nerve graft. After phrenic nerve transfer to the musculocutaneous nerve, the regenerated potentials first appeared at 3 months. Recovery of motor unit action potential occurred 6 months later and became more apparent at 12 months. The percent of patients recovering ′excellent′ and ′good′ muscle strength in the biceps brachii was 80% after 18 months. At 12 months after surgery, motor nerve conduction potential appeared in the musculocutaneous nerve in seven cases. These data suggest that preoperative evaluation of phrenic nerve function may help identify the most appropriate nerve graft in patients with an injured brachial plexus. The functional recovery of a transplanted nerve can be dynamically observed after the surgery.

DNA-binding proteins play an important role in cell metabolism. In biological laboratories, the detection methods of DNA-binding proteins includes yeast one-hybrid methods, bacterial singles and X-ray crystallography methods and others, but these methods involve a lot of labor, material and time. In recent years, many computation-based approachs have been proposed to detect DNA-binding proteins. In this paper, a machine learning-based method, which is called the Fuzzy Kernel Ridge Regression model based on Multi-View Sequence Features (FKRR-MVSF), is proposed to identifying DNA-binding proteins. First of all, multi-view sequence features are extracted from protein sequences. Next, a Multiple Kernel Learning (MKL) algorithm is employed to combine multiple features. Finally, a Fuzzy Kernel Ridge Regression (FKRR) model is built to detect DNA-binding proteins. Compared with other methods, our model achieves good results. Our method obtains an accuracy of 83.26% and 81.72% on two benchmark datasets (PDB1075 and compared with PDB186), respectively.

Time-reversal symmetry (TRS) of electrons is associated with an anti-unitary operator with T 2 = − 1 , which induces Kramers degeneracy and plays an important role in realizing the quantum spin Hall effect (QSHE). By contrast, TRS of photons is described by T b 2 = 1 . We point out that due to this difference, TRS is not the necessary condition for the construction of the photonic analogue of the QSHE. Instead, by constructing an artificial pseudo TRS T p with T p 2 = − 1 in a photonic system, one can realize the photonic Kramers degeneracy and a pair of topological protected edge states, a photonic analogue of the QSHE. Specifically, by retrieving the optical parameters of materials with the pseudo TRS, we propose a photonic topological insulator (PTI) utilizing a pair of double-degenerate transverse electric (TE) and transverse magnetic (TM) polarizations to mimic the spin up and down states of the electron. We demonstrate that the unidirectional polarization-dependent transportation of TE and TM edge states can be realized in this system based on computer simulations. For all possible symmetry types, we check the robustness of these topological states by using a complete set of impurities, including three Pauli matrices and one complex conjugate operator. The results show that the PTI is protected by the pseudo TRS T p . In general, an arbitrary pair of optical polarizations on the Bloch sphere can be utilized to construct photonic pseudospin states and the PTI. Our findings confirm the physical meaning of the pseudo TRS and may provide guidance for future PTI designs.

Online multi-object tracking (MOT) has broad applications in time-critical video analysis scenarios such as advanced driver-assistance systems (ADASs) and autonomous driving. In this paper, the proposed system aims at tracking multiple vehicles in the front view of an onboard monocular camera. The vehicle detection probes are customized to generate high precision detection, which plays a basic role in the following tracking-by-detection method. A novel Siamese network with a spatial pyramid pooling (SPP) layer is applied to calculate pairwise appearance similarity. The motion model captured from the refined bounding box provides the relative movements and aspects. The online-learned policy treats each tracking period as a Markov decision process (MDP) to maintain long-term, robust tracking. The proposed method is validated in a moving vehicle with an onboard NVIDIA Jetson TX2 and returns real-time speeds. Compared with other methods on KITTI and self-collected datasets, our method achieves significant performance in terms of the “Mostly-tracked”, “Fragmentation”, and “ID switch” variables.

In order to realize rapid THz detecting and imaging, a line beam scanning-based ultra-fast THz imaging platform is designed combining simple optical components and lightweight mechanical system. The designed THz imaging platform has the resolution of 12 mm, the scanning angle range of ±10.5°, the scanning speed of 0.17 s/frame, and the scanning range of 2 m × 0.8 m; moreover, it can realize rapid human body THz imaging and distinguish metallic objects. Considering its high-quality performance in THz imaging and detecting, it is believed the proposed line beam scanning-based ultra-fast THz imaging platform can be used in the future in various safe screening applications.

Exposure to fine particles can cause various diseases, and an easily accessible method to monitor the particles can help raise public awareness and reduce harmful exposures. Here we report a method to estimate PM air pollution based on analysis of a large number of outdoor images available for Beijing, Shanghai (China) and Phoenix (US). Six image features were extracted from the images, which were used, together with other relevant data, such as the position of the sun, date, time, geographic information and weather conditions, to predict PM2.5 index. The results demonstrate that the image analysis method provides good prediction of PM2.5 indexes, and different features have different significance levels in the prediction.

A series of 3'-aminospiro[indoline-3,1'-pyrazolo[1,2-b]phthalazine]-2,5',10'-trione derivatives have been synthesized by a one-pot three-component reaction of isatin, malononitrile or ethyl cyanoacetate and phthalhydrazide catalyzed by piperidine under ultrasound irradiation. For comparison the reactions were carried out under both conventional and ultrasonic conditions. In general, improvement in rates and yields were observed when the reactions were carried out under sonication compared with classical conditions.

Oregano is an aromatic plant widely distributed throughout the Mediterranean area and in Asia. Recent studies have revealed that the anti-inflammatory effect of essential oil in this plant. However, the mechanisms underlying the therapeutic potential have not been well elucidated. This study determined whether oregano essential oil (OEO) exerts an anti-inflammatory effect on lipopolysaccharide (LPS)-treated murine macrophage cells (RAW264.7 cells) in vitro and elucidated the possible underlying molecular mechanisms. The results showed that OEO (2.5–10 μg/mL) inhibited the expression and secretion of interleukin-1 beta (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor-alpha (TNF-α) in RAW264.7 cells treated with LPS (1 μg/mL). Consistent with the pro-inflammatory gene expression, the OEO treatment efficiently reduced the LPS-induced activation of mitogen-activated protein kinase, protein kinase B, and nuclear factor κB in RAW264.7 cells. Nicotinamide adenine dinucleotide phosphate (NADPH) oxidase inhibition in Nox2 protein-silenced cells attenuated the mRNA expression of IL-1β, IL-6, and TNF-α in the LPS-induced RAW264.7 cells. The OEO inhibited the LPS-induced elevation of NADPH oxidase and oxidative stress. This result suggests that LPS induces RAW264.7 cell inflammation through the NADPH oxidase-mediated production of reactive oxygen species (ROS). In conclusion, OEO protects against the LPS-induced RAW264.7 cell inflammatory response through the NADPH oxidase/ROS pathway.

BACKGROUND:A wealth of evidence from randomized controlled trials (RCTs) has indicated that hypertonic saline (HS) is at least as effective as, if not better than, mannitol in the treatment of increased intracranial pressure(ICP). However, there is little known about the effects of HS in patients during neurosurgery. Thus, this meta-analysis was performed to compare the intraoperative effects of HS with mannitol in patients undergoing craniotomy. METHODS:According to the research strategy, we searched PUBMED, EMBASE and Cochrane Central Register of Controlled Trials. Other sources such as the internet-based clinical trial registries and conference proceedings were also searched. After literature searching, two investigators independently performed literature screening, quality assessment of the included trials and data extraction. The outcomes included intraoperative brain relaxation, intraoperative ICP, total volume of fluid required, diuresis, hemodynamic parameters, electrolyte level, mortality or dependence and adverse events. RESULTS:Seven RCTs with 468 participants were included. The quality of the included trials was acceptable. HS could significantly increase the odds of satisfactory intraoperative brain relaxation (OR: 2.25, 95% CI: 1.32-3.81; P = 0.003) and decrease the mean difference (MD) of maximal ICP (MD: -2.51 mmHg, 95% CI: -3.39--1.93 mmHg; P

Adaptive behavior depends less on the details of the negotiation process and makes more robust predictions in the long term as compared to in the short term. However, the extant literature on population dynamics for behavior adjustment has only examined the current situation. To offset this limitation, we propose a synergy of evolutionary algorithm and reinforcement learning to investigate long-term collective performance and strategy evolution. The model adopts reinforcement learning with a tradeoff between historical and current information to make decisions when the strategies of agents evolve through repeated interactions. The results demonstrate that the strategies in populations converge to stable states, and the agents gradually form steady negotiation habits. Agents that adopt reinforcement learning perform better in payoff, fairness, and stableness than their counterparts using classic evolutionary algorithm.

In the biosynthesis of polyketides, ketoreductases (KRs) are an important group of enzymes that determine the chiralities of the carbon backbones. SiaM is a special member of this group that can recognize substrates with different lengths and can be used iteratively. Here we report the crystal structure of SiaM. Structural analysis indicates that the overall structure resembles those of other KRs. However, significant disparity can be found in the conserved LDD motif that is replaced with IRD motif in SiaM. The isoleucine and aspartic acid residues take similar orientations as leucine and aspartic acid in the conserved LDD motif, while the arginine residue points out towards the solvent. PISA analysis shows that SiaM forms a tetramer. Several aromatic residues are found in the interfaces, which have aromatic stacking interactions with the aromatic residues in the neighboring protomers. Mutagenesis studies performed on the aromatic residues show that these sites are important for maintaining the structural integrity of SiaM. However, the aromatic residues contribute differently to the enzymatic activity. In the N-terminal interface, the aromatic residues can be replaced with leucine without affecting the enzymatic activity while, in the other interface, such mutations abolish the enzymatic activity.

A positive relationship between plant diversity and both abundance and diversity of predatory arthropods is postulated by the Enemies Hypothesis, a central ecological top-down control hypothesis. It has been supported by experimental studies and investigations of agricultural and grassland ecosystems, while evidence from more complex mature forest ecosystems is limited. Our study was conducted on Changbai Mountain in one of the last remaining large pristine temperate forest environments in China. We used predatory ground beetles (Coleoptera: Carabidae) as target taxon to establish the relationship between phytodiversity and their activity abundance and diversity. Results showed that elevation was the only variable included in both models predicting carabid activity abundance and α-diversity. Shrub diversity was negatively and herb diversity positively correlated with beetle abundance, while shrub diversity was positively correlated with beetle α-diversity. Within the different forest types, a negative relationship between plant diversity and carabid activity abundance was observed, which stands in direct contrast to the Enemies Hypothesis. Furthermore, plant species density did not predict carabid α-diversity. In addition, the density of herbs, which is commonly believed to influence carabid movement, had little impact on the beetle activity abundance recorded on Changbai Mountain. Our study indicates that in a relatively large and heterogeneous mature forest area, relationships between plant and carabid diversity are driven by variations in environmental factors linked with altitudinal change. In addition, traditional top-down control theories that are suitable in explaining diversity patterns in ecosystems of low diversity appear to play a much less pronounced role in highly complex forest ecosystems.

Recepteur d'origine nantais (Ron) is overexpressed in a panel of pancreatic cancer cells and tissue samples from pancreatic cancer patients. Ron can be activated by its ligand macrophage stimulating protein (MSP), thereby activating oncogenic signaling pathways. Crosstalk between Ron and EGFR, c-Met, or IGF-1R may provide a mechanism underlying drug resistance. Thus, targeting Ron may represent a novel therapeutic strategy. IMC-RON8 is the first Ron monoclonal antibody (mAb) entering clinical trial for targeting Ron overexpression. Our studies show IMC-RON8 downmodulated Ron expression in pancreatic cancer cells and significantly blocked MSP-stimulated Ron activation, downstream Akt and ERK phosphorylation, and survivin mRNA expression. IMC-RON8 hindered MSP-induced cell migration and reduced cell transformation. Histone deacetylase inhibitors (HDACi) are reported to target expression of various genes through modification of nucleosome histones and non-histone proteins. Our work shows HDACi TSA and Panobinostat (PS) decreased Ron mRNA and protein expression in pancreatic cancer cells. PS also reduced downstream signaling of pAkt, survivin, and XIAP, as well as enhanced cell apoptosis. Interestingly, PS reduced colony formation in Ron knockdown cells to a greater extent than Ron scramble control cells in colony formation and soft agarose assays. IMC-RON8 could also sensitize pancreatic cancer cells to PS, as reflected by reduced colony numbers and size in combination treatment with IMC-RON8 and PS compared to single treatment alone. The co-treatment further reduced Ron expression and pAkt, and increased PARP cleavage compared to either treatment alone. This study suggests the potential for a novel combination approach which may ultimately be of value in treatment of pancreatic cancer.

The ever-increasing design complexity of modern digital systems makes it necessary to develop electronic system-level (ESL) methodologies with automation and optimization in the higher abstraction level. How the concurrency is modeled in the application specification plays a significant role in ESL design frameworks. The state-of-art concurrent specification models are not suitable for modeling task-level concurrent behavior for the hardware synthesis design flow. Based on the concurrent collection (CnC) model, which provides the maximum freedom of task rescheduling, we propose task-level data model (TLDM), targeted at the task-level optimization in hardware synthesis for data processing applications. Polyhedral models are embedded in TLDM for concise expression of task instances, array accesses, and dependencies. Examples are shown to illustrate the advantages of our TLDM specification compared to other widely used concurrency specifications.

Animal models are widely used in laboratory practicals in biomedical disciplines. However, experiments involving animal work are usually time-and-labor consuming. It is also costly to run an animal lab and the animal caring/holding request special training. Here we present a laboratory course design that provides students with wet lab experience in gene editing in mouse stem cell lines using CRSISPR/Cas9 and dry lab experience in generating transgenic mouse models using computer simulation. This workshop is for senior undergraduates as well as graduate students and is designed to help them understand the application of gene editing in biomedical research. The virtual simulation is incorporated as a computer-based alternative to using mice for embryo transplantation, breeding, and characterization. The integrated curriculum of wet and dry laboratories enabled us to complete this complicate multi-disciplinary experiment in one semester. In addition, the computer-based alternatives reduce the unnecessary animal tests down to zero and errors during practice are well-tolerated without raising any ethical issues. The survey and student's feedback showed overall satisfaction in their learning experiences and learning benefits. The evaluation of the outcomes of learning objectives on the completion of this course revealed positive achievements in bioinformatics, molecular biology, and in general animal work