241 results on '"Yaping Pan"'
Search Results
102. Temperature mediates metabolism switching of Bacillus sp. ZT-1: Analysis of the properties and structure of exopolysaccharides
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Hongrui Fu, Wei Liu, Mutai Bao, Xiaojun Sun, Yaping Pan, Xin Hu, and Xiuli Zhang
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Polysaccharides, Bacterial ,Temperature ,Mannose ,Bacillus ,Metabolism ,Carbohydrate ,Biology ,Carbohydrate metabolism ,Pentose phosphate pathway ,Microbiology ,High-performance liquid chromatography ,Biological pathway ,chemistry.chemical_compound ,Biochemistry ,chemistry ,Sugar - Abstract
Microorganism was very sensitive to external temperature change, which also affected its normal metabolism and secretion. Low temperature exopolysaccharides (LT-EPS) and normal temperature exopolysaccharides (NT-EPS) secreted by Bacillus sp. ZT-1 mediated by temperature were studied in this paper. The total carbohydrate in the LT-EPS and NT-EPS were found to be 82.54 ± 2.56 % and 94.23 ± 1.59 % (w/w). The High Performance Liquid Chromatography (HPLC) revealed the mannose and galacturonic acid accounted for 45.52 and 23.49 % in LT-EPS, respectively. In like manner, mannose and galacturonic acid contained 43.99 and 25.24 % in NT-EPS. One-dimensional nuclear magnetic resonance (NMR) revealed the connection mode of sugar chains. NT-EPS exhibited higher viscosity, better emulsification properties and the larger molecular weight than LT-EPS. Scanning electron microscopy (SEM) showed that LT-EPS was sheet-like with sugar chain branches while NT-EPS was showed as network structure. Furthermore, the 2812 differentially expressed genes (DEGs) were analyzed by Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment and located 739 biological pathways. Finally, transcriptome analysis revealed differences in gene expression of the pentose phosphate pathway of carbohydrate metabolism might be the main reason for this difference.
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- 2021
103. Superhydrophobic magnetic cotton fabricated under low carbonization temperature for effective oil/water separation
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Yang Li, Mutai Bao, Yaping Pan, and Zichao Yin
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Materials science ,Carbonization ,Filtration and Separation ,02 engineering and technology ,021001 nanoscience & nanotechnology ,Environmentally friendly ,Analytical Chemistry ,law.invention ,Contact angle ,Oleic acid ,chemistry.chemical_compound ,Adsorption ,020401 chemical engineering ,chemistry ,Chemical engineering ,law ,Thermal stability ,0204 chemical engineering ,0210 nano-technology ,Porosity ,Distillation - Abstract
Carbonization of bio-based porous substrates is a facile, environmentally friendly and cost-effective way to create hydrophobic absorbents for oil/water separation applications. However, the processes usually take place under high temperature, which can cause large consumption of energy. Therefore, it is still a challenge to fabricate such absorbent under low temperature. Herein, a kind of superhydrophobic magnetic cotton was successfully fabricated by dip-coating of cotton with Fe3O4 nanoparticles, adsorption of oleic acid and carbonization at 400 °C. The prepared absorbent was integrated with various excellent properties after modifications, including low apparent density (0.0436 g/cm3), high porosity (92.2%), remote controllability, excellent thermal stability and outstanding hydrophobicity (water contact angle, 157.0 ± 1.8°). It could selectively collect oil from different types of oil/water mixtures with large absorption capacity (13.63–37.39 g/g for the testing oils) and high separation efficiency (higher than 99%). After removed the absorbed oil by distillation or direct combustion, the absorbent could be used again with no obvious performance deterioration. The proposed method offers an energy-saving way to fabricate oil absorbent via carbonization.
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- 2021
104. A Sialidase-DeficientPorphyromonas gingivalisMutant Strain Induces Less Interleukin-1β and Tumor Necrosis Factor-α in Epi4 Cells Than W83 Strain Through Regulation of c-Jun N-Terminal Kinase Pathway
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Jingbo Liu, Dongmei Zhang, Li Lin, Xiaoyu Xu, Ning Yu, Xue Yang, Xiaolin Tang, Yaping Pan, Tong Tong, and Chen Li
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Lipopolysaccharides ,0301 basic medicine ,Lipopolysaccharide ,Virulence Factors ,Interleukin-1beta ,030106 microbiology ,Neuraminidase ,Virulence ,Biology ,Real-Time Polymerase Chain Reaction ,Sialidase ,Cell Line ,Microbiology ,03 medical and health sciences ,chemistry.chemical_compound ,Adhesins, Bacterial ,Porphyromonas gingivalis ,Microbial Viability ,Tumor Necrosis Factor-alpha ,Kinase ,c-jun ,JNK Mitogen-Activated Protein Kinases ,Interleukin ,biology.organism_classification ,Cysteine Endopeptidases ,chemistry ,Genes, Bacterial ,Mutation ,Gingipain Cysteine Endopeptidases ,Periodontics ,Tumor necrosis factor alpha - Abstract
Porphyromonas gingivalis is one of the major periodontal pathogens. In a previous study, a mouse abscess model showed that sialidase deficiency of P. gingivalis weakened its virulence, but the mechanism behind this observation remains unknown.A sialidase-deficient mutant strain (△PG0352) and a complemented strain (com△PG0352) were constructed. Epi4 cells were stimulated by wild-type strain P. gingivalis W83, △PG0352, or com△PG0352. Real-time polymerase chain reaction was carried out to detect expression of virulent genes in P. gingivalis and interleukin (IL)-1β, IL-6, IL-8, and tumor necrosis factor (TNF)-α in epi4 cells. Activities of sialidase, gingipains, and lipopolysaccharide (LPS) were compared among the different P. gingivalis strains. Levels of IL-1β and TNF-α in the epi4 cells supernatant were detected by enzyme-linked immunosorbent assay and levels of p38, extracellular signal-regulated kinase, c-Jun N-terminal kinase (JNK), and phospho-c-Jun were detected by western blotting.Compared with P. gingivalis W83 and com△PG0352, activities of Kgp and Rgp gingipains and amount of LPS decreased in △PG0352, whereas there were no differences in LPS activity among these three strains. Level of phospho-JNK was lower in epi4 cells stimulated by △PG0352. △pG0352 induced less IL-1β and TNF-α and more IL-8 in epi4 cells; differences in IL-1β and TNF-α could not be detected after JNK blocking.A sialidase-deficient P. gingivalis mutant strain induces less IL-1β and TNF-α in epi4 cells than W83 strain through regulation of JNK pathway.
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- 2017
105. Preventive effects of the novel antimicrobial peptide Nal-P-113 in a rat Periodontitis model by limiting the growth of Porphyromonas gingivalis and modulating IL-1β and TNF-α production
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Jya-Wei Cheng, Ning Yu, Wei Fu, Yaping Pan, Hui-Yuan Yu, Li Lin, Lisi Tan, and Hongyan Wang
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Male ,0301 basic medicine ,Saliva ,Interleukin-1beta ,Porphyromonas ,Microbiology ,Rats, Sprague-Dawley ,03 medical and health sciences ,0302 clinical medicine ,Western blot ,In vivo ,medicine ,Animals ,Humans ,Animal model ,Periodontitis ,Porphyromonas gingivalis ,medicine.diagnostic_test ,biology ,Tumor Necrosis Factor-alpha ,Chemistry ,Alveolar bone loss ,Inflammatory response ,030206 dentistry ,General Medicine ,lcsh:Other systems of medicine ,medicine.disease ,Antimicrobial ,biology.organism_classification ,lcsh:RZ201-999 ,Rats ,Disease Models, Animal ,030104 developmental biology ,Complementary and alternative medicine ,Histatin ,Peptides ,Antimicrobial peptide ,Research Article - Abstract
Background P-113 (AKRHHGYKRKFH-NH2) is a 12-amino-acid histidine-rich peptide derived from histatin 5 that is highly degradable in high salt concentrations and biological fluids such as serum, plasma and saliva. Nal-P-113, a novel antimicrobial peptide whose histidine residues are replaced by the bulky amino acids β-naphthylalanine, causes the antimicrobial peptide to retain its bactericidal activity even in physiological environments. This study evaluated the effect of the novel antimicrobial peptide Nal-P-113 in a rat periodontitis model and the mechanisms of action of Nal-P-113 for suppressing periodontitis. Methods Periodontitis was induced in mandibular first molars in rats receiving a ligature and infected with Porphyromonas gingivalis. Animals were randomly divided into six groups: a, P. gingivalis W83 alone; b, P. gingivalis W83 with 6.25 μg/mL of Nal-P-113; c, P. gingivalis W83 with 25 μg/mL of Nal-P-113; d, P. gingivalis W83 with 100 μg/mL of Nal-P-113; e, P. gingivalis W83 with 400 μg/mL of Nal-P-113; and f, control without P. gingivalis W83 or Nal-P-113. Morphometric analysis was used to evaluate alveolar bone loss. Microbiological assessment of the presence of Porphyromonas gingivalis and total bacteria was performed using absolute quantitative real-time PCR and scanning electron microscopy. Gingival tissue was collected for western blot and immunohistochemical assays of IL-1β and TNF-α levels. Results Alveolar bone loss was inhibited by 100 μg/mL or 400 μg/mL of Nal-P-113 compared to the control group (P
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- 2017
106. Composition and function of oral microbiota between gingival squamous cell carcinoma and periodontitis
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Zhongfei Xu, Xuexin Tan, Shuwei Zhang, Ze Lu, Weiyi Duan, Yuchao Li, Yaping Pan, Xida Zhao, Ran Yin, Enjiao Zhang, Xiaoting Shi, Junchao Liu, Wei Dai, and Shao Hui Huang
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Gingival Squamous Cell Carcinoma ,Male ,Cancer Research ,Saliva ,Pathology ,medicine.medical_specialty ,Atopobium ,Gingiva ,medicine.disease_cause ,03 medical and health sciences ,0302 clinical medicine ,stomatognathic system ,Prevotella ,Medicine ,Humans ,030223 otorhinolaryngology ,Periodontitis ,biology ,business.industry ,Streptococcus ,Microbiota ,Middle Aged ,biology.organism_classification ,medicine.disease ,Peptostreptococcus ,stomatognathic diseases ,Oncology ,Fusobacterium ,030220 oncology & carcinogenesis ,Carcinoma, Squamous Cell ,Female ,Mouth Neoplasms ,Oral Surgery ,business - Abstract
Objectives Previous studies have proved that periodontitis is an independent risk factor of oral squamous cell carcinoma (OSCC) epidemiologically. Along with the important role of microbiota in the cancer process and the specific anatomical position, our study explored the microbial composition and functions in periodontitis and gingival squamous cell carcinoma (GSCC). Materials and Methods GSCC patients (n = 10), matched periodontitis patients (n = 15), and healthy individuals (n = 15) were recruited. Saliva, subgingival plaque, tongue dorsum, buccal mucosa, cancerous tissue, and paracancerous tissue samples were collected. 16S rDNA amplicon sequencing and functional prediction were applied for the taxonomic analysis. Results Periodontal pathogens occupied 46% in GSCC. Besides, the mutual operational taxonomy unites (OTU) generated from the subgingival plaque occupied 38.36% and 44.13% from saliva. Fusobacterium, Peptostreptococcus, and Prevotella were more abundant in cancerous tissues, while Streptococcus, Neisseria, and Haemophilus were more enriched in saliva or soft mucosa. PCoA exhibited similar cluster between tongue dorsum and saliva in GSCC. GSCC showed lower richness than periodontitis. In saliva and subgingival plaque, Atopobium was more prevalent in GSCC than periodontitis and controls in descending order. Lipopolysaccharide (LPS) biosynthesis increased in subgingival plaque of GSCC compared with the other two groups. Conclusion Periodontal pathogens were abundant in GSCC. Cancerous tissues harbor enriched periodontal pathogens while saliva or soft mucosa harbored more periodontal health related bacteria. A high level of Atopobium in saliva and LPS biosynthesis have the potential for increasing the risk of suffering from GSCC in individuals with periodontitis, which needs more evidence to clarify it.
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- 2019
107. The dimension and morphology of alveolar bone at maxillary anterior teeth in periodontitis: a retrospective analysis-using CBCT
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Ziming Ge, Yuchao Li, Yaping Pan, Lei Miao, Xue Zhang, and Haijiao Zhao
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Adult ,Male ,Dentistry ,Tooth Cervix ,Article ,stomatognathic system ,Tooth Apex ,Alveolar ridge ,Retrospective analysis ,Alveolar Process ,Maxilla ,Medicine ,Humans ,Oral manifestations ,Periodontitis ,General Dentistry ,Anterior teeth ,Dental alveolus ,Retrospective Studies ,business.industry ,Cone-Beam Computed Tomography ,medicine.disease ,Sagittal plane ,Clinical Practice ,lcsh:RK1-715 ,Incisor ,stomatognathic diseases ,medicine.anatomical_structure ,lcsh:Dentistry ,Female ,business ,Fenestration - Abstract
The morphology of the alveolar bone at the maxillary anterior teeth in periodontitis patients was evaluated by cone-beam computed tomography (CBCT) to investigate the distribution of alveolar defects and provide guidance for clinical practice. Ninety periodontitis patients and 30 periodontally healthy individuals were selected to determine the morphology of the alveolar bone at the maxillary anterior teeth according to the degree of bone loss, tooth type, sex and age. The differences in the dimensions between periodontitis patients and healthy individuals were compared, and the distribution of alveolar bone defects was analyzed. A classification system was established regarding the sagittal positions and angulations of the teeth. The buccal residual bone was thicker and the lingual bone was thinner in the periodontitis patients than in the periodontally healthy individuals, and there were differences between the different tooth types, sexes and age subgroups. The buccal undercut was close to the alveolar ridge, while fenestration was reduced and the apical bone height was higher in periodontitis patients than in periodontally healthy individuals. The apical bone height increased with the aggravation of bone loss and age. The proportions of different sagittal positions changed with the aggravation of bone loss. Moreover, the teeth moved more buccally regarding the positions of the maxillary anterior teeth. The morphology of the alveolar bone at the maxillary anterior teeth differed between periodontitis patients and healthy individuals, and the differences were related to the degree of bone loss, tooth type, sex and age.
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- 2019
108. Periodontal disease and Helicobacter pylori infection in oral cavity: a meta-analysis of 2727 participants mainly based on Asian studies
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Xiaomeng Xue, Yanqing Liu, Jingbo Liu, Junchao Liu, Rong Li, Yaping Pan, Tong Xu, Qin Dong, Dongmei Zhang, and Yuxiao Luo
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medicine.medical_specialty ,Asia ,Cochrane Library ,Helicobacter Infections ,03 medical and health sciences ,0302 clinical medicine ,Periodontal disease ,Internal medicine ,Medicine ,Humans ,General Dentistry ,Periodontal Diseases ,biology ,Helicobacter pylori ,business.industry ,030206 dentistry ,Odds ratio ,Publication bias ,biology.organism_classification ,Confidence interval ,Europe ,030220 oncology & carcinogenesis ,Meta-analysis ,Observational study ,business - Abstract
To assess the association between periodontal disease and Helicobacter pylori (H. pylori) infection in oral cavity. We searched PubMed, Embase, Web of Science, Cochrane library, Gray literature, and clinicaltrials.gov for eligible studies up to September 25, 2019. Odds ratios (ORs) and 95% confidence intervals (CIs) were calculated. The random-effects model was used with the software STATA 13.0. The Newcastle-Ottawa-Scale was used for quality evaluation. Twelve observational studies (eight from Asia, one from Europe, and three from the South America) involving 2727 participants were included in the meta-analysis. The overall pooled results showed that H. pylori infection in oral cavity was associated with periodontal disease (OR 2.53, 95% CI 1.86–3.44, P
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- 2019
109. A long-lived Donor-Acceptor fluorescent probe for sequential detection of Cu
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Duobin, Chao, Yaping, Pan, and Xue-Wang, Gao
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Luminescence ,Spectrometry, Fluorescence ,Humans ,Sulfhydryl Compounds ,Copper ,Fluorescent Dyes ,HeLa Cells - Abstract
A new long-lived Donor-Acceptor (D-A) fluorophore based on carbazolyl dicyanobenzene was developed as an ON-OFF-ON multifunctional fluorescent probe 1 for sequential detection of Cu
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- 2019
110. The expression of macrophage migration inhibitory factor and intercellular adhesion molecule-1 in rats with periodontitis and atherosclerosis
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Xiaomeng Xue, Yaping Pan, Rong Li, Yun Wu, Tong Xu, Yanqing Liu, Jingya Hou, and Dongmei Zhang
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0301 basic medicine ,Male ,medicine.medical_specialty ,Carotid arteries ,Intercellular Adhesion Molecule-1 ,Protein expression ,Rats, Sprague-Dawley ,03 medical and health sciences ,Random Allocation ,0302 clinical medicine ,Internal medicine ,medicine ,Animals ,Periodontitis ,General Dentistry ,Macrophage Migration-Inhibitory Factors ,ICAM-1 ,business.industry ,030206 dentistry ,Cell Biology ,General Medicine ,medicine.disease ,Intercellular adhesion molecule ,Atherosclerosis ,Rats ,Intramolecular Oxidoreductases ,030104 developmental biology ,Endocrinology ,Otorhinolaryngology ,Immunohistochemistry ,Macrophage migration inhibitory factor ,business - Abstract
The aim of this study was to explore the role of macrophage migration inhibitory factor (MIF) and intercellular adhesion molecule (ICAM)-1 in rats with periodontitis and atherosclerosis.Twenty-four male Sprague-Dawley rats were randomly divided into 4 groups: control group (C), periodontitis group (P), atherosclerosis group (AS), and periodontitis plus atherosclerosis group (P + AS). The levels of MIF and ICAM-1 in serum were detected by an enzyme-linked immunosorbent assay (ELISA). The protein expression of MIF and ICAM-1 in the carotid artery tissues was examined by immunohistochemical staining.The results of the ELISA showed that the serum level of MIF in the P + AS group (59.40 ± 3.92 ng/mL) was significantly higher than that in the C group (42.93 ± 2.63 ng/mL), the P group (45.57 ± 2.59 ng/mL) and the AS group (50.88 ± 4.20 ng/mL) (P 0.05). Similarly, the ICAM-1 level in the P + AS group (6.77 ± 1.47 ng/mL) was much higher than that in the C (1.33 ± 0.25 ng/mL), P (3.99 ± 0.44 ng/mL) and AS groups (4.19 ± 0.89 ng/mL) (P 0.05). Furthermore, the expression of MIF, as assessed by immunohistochemical staining, was significantly higher in the artery tissues of the P + AS group than in the tissues of the other three groups (P 0.05). However, there was no significant difference in the protein level of ICAM-1 between the P + AS and AS groups, where its expression was much stronger than in the C and the P groups.Our results indicate that there is a close association between periodontitis and atherosclerosis. MIF and ICAM-1 may play a role in the development of atherosclerosis and periodontitis.
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- 2019
111. miR-155 promotes macrophage pyroptosis induced by Porphyromonas gingivalis through regulating the NLRP3 inflammasome
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Chen Li, Junchao Liu, Yaping Pan, Ning Yu, Dongmei Zhang, Wanting Yin, Li Lin, Haijiao Zhao, and Jingbo Liu
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Inflammasomes ,medicine.medical_treatment ,Interleukin-1beta ,03 medical and health sciences ,0302 clinical medicine ,NLR Family, Pyrin Domain-Containing 3 Protein ,medicine ,Pyroptosis ,Macrophage ,Humans ,General Dentistry ,Porphyromonas gingivalis ,biology ,U937 cell ,Chemistry ,Suppressor of cytokine signaling 1 ,Monocyte ,Macrophages ,Inflammasome ,030206 dentistry ,biology.organism_classification ,Molecular biology ,Neoplasm Proteins ,MicroRNAs ,medicine.anatomical_structure ,Cytokine ,Otorhinolaryngology ,030220 oncology & carcinogenesis ,medicine.drug - Abstract
Objective The aim of this study is to detect pyroptosis in macrophages stimulated with Porphyromonas gingivalis and elucidate the mechanism by which P. gingivalis induces pyroptosis in macrophages. Methods The immortalized human monocyte cell line U937 was stimulated with P. gingivalis W83. Flow cytometry was carried out to detect pyroptosis in macrophages. The expression of miR-155 was detected by real-time PCR and inhibited using RNAi. Suppressor of cytokine signaling (SOCS) 1, cleaved GSDMD, caspase (CAS)-1, caspase-11, apoptosis-associated speck-like protein (ASC), and NOD-like receptor protein 3 (NLRP3) were detected by Western blotting, and IL-1β and IL-18 were detected by ELISA. Results The rate of pyroptosis in macrophages and the expression of miR-155 increased upon stimulation with P. gingivalis and pyroptosis rate decreased when miR-155 was silenced. GSDMD-NT, CAS-11, CAS-1, ASC, NLRP3, IL-1β, and IL-18 levels increased, but SOCS1 decreased in U937 cells after stimulated with P. gingivalis. These changes were weakened in P. gingivalis-stimulated U937 macrophages transfected with lentiviruses carrying miR-155 shRNA compared to those transfected with non-targeting control sequence. However, there was no significant difference in ASC expression between P. gingivalis-stimulated shCont and shMiR-155 cells. Conclusions Porphyromonas gingivalis promotes pyroptosis in macrophages during early infection. miR-155 is involved in this process through regulating the NLRP3 inflammasome.
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- 2019
112. Association between Periodontitis and Gene polymorphisms of hBD-1 and CD14: a meta-analysis
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Li Lin, Chen Chen, Yaping Pan, Peicheng Liu, Chen Li, Xiaomiao Fan, and Shiwen Yu
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0301 basic medicine ,Risk ,medicine.medical_specialty ,beta-Defensins ,CD14 ,Lipopolysaccharide Receptors ,Gastroenterology ,Polymorphism, Single Nucleotide ,03 medical and health sciences ,0302 clinical medicine ,Polymorphism (computer science) ,Internal medicine ,medicine ,Aggressive periodontitis ,Humans ,Genetic Predisposition to Disease ,Periodontitis ,General Dentistry ,Gene ,business.industry ,Data synthesis ,030206 dentistry ,Cell Biology ,General Medicine ,medicine.disease ,030104 developmental biology ,Otorhinolaryngology ,Meta-analysis ,Case-Control Studies ,Gene polymorphism ,business - Abstract
Objectives The aim of this meta-analysis was to analyze the association between periodontitis risk and gene polymorphisms of hBD-1 (rs11362, rs1799946 and rs1800972) and CD14 (rs2569190) by data synthesis. Methods This meta-analysis was performed using the PubMed and China National Knowledge Infrastructure databases and included 18 case–control studies. Statistical analyses were completed with Stata 12.0. Results In the overall analysis, there was no significant association between DEFB1 polymorphisms (rs11362, rs1799946 and rs1800972) and periodontitis risk. However, when examined by ethnicity, rs11362 (AG + AA vs GG: pooled OR = 3.561, 95% CI = 1.986–6.386, P = 0.000), rs1800972 (GC vs CC: pooled OR=0.391, 95% CI=0.216-0.708, P = 0.002; G vs C: pooled OR = 0.540, 95% CI = 0.337-0.867, P = 0.011) and rs1799946 (AG+AA vs GG: pooled OR=1.995, 95% CI=1.163-3.422, P = 0.012) polymorphisms were associated with periodontitis risk in Asian. Similarly, rs11362 and rs1799946 polymorphisms were related to periodontitis risk in Brazilian. In the stratified analysis by type of disease, rs1799946 polymorphism (AA vs GG: OR=1.444, 95% CI=1.051-1.983, P = 0.023; AG+AA vs GG: OR=1.374, 95% CI=1.021-1.849, P = 0.036; A vs G: OR=1.172, 95% CI=1.012-1.358, P = 0.034) and rs1800972 polymorphisms (GC vs CC: OR = 0.790, 95% CI = 0.638-0.979, P = 0.031; GG vs CC: OR=0.542, 95% CI=0.316-0.930, P = 0.026; GC+GG vs CC: OR=0.759, 95% CI=0.617-0.933, P = 0.009; G vs C: OR=0.773, 95% CI=0.649-0.921, P = 0.004) had significant associations with aggressive periodontitis (AP) risk. Nevertheless, in the overall and stratified analysis by the severity of periodontitis and ethnicity, no significant association was discovered between CD14 polymorphisms and periodontitis. Conclusions This meta-analysis demonstrated that gene polymorphism of DEFB1 but not of CD14 might be involved in periodontitis risk.
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- 2019
113. Identification of Potential Candidate Genes of Oral Cancer in Response to Chronic Infection With Porphyromonas gingivalis Using Bioinformatical Analyses
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Dongmei Zhang, Yaping Pan, Qingxuan Wang, Junchao Liu, Shuwei Zhang, Fengxue Geng, and Chen Li
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0301 basic medicine ,Cancer Research ,Tumor initiation ,Biology ,lcsh:RC254-282 ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Porphyromonas gingivalis ,Original Research ,Microarray analysis techniques ,Head and neck cancer ,Cancer ,bioinformatics ,oral cancer ,chronic infection ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,medicine.disease ,biology.organism_classification ,Chronic periodontitis ,Head and neck squamous-cell carcinoma ,Chronic infection ,030104 developmental biology ,Oncology ,inflammation ,030220 oncology & carcinogenesis ,Cancer research - Abstract
Recent investigations revealed the relationship between chronic periodontitis, Porphyromonas gingivalis and cancer. However, host genes that change in response to chronic infection with P. gingivalis and may contribute to oral cancer have remained largely unknown. In the present study, we aimed to comprehensively analyze microarray data obtained from the chronic infection model of immortalized oral epithelial cells that were persistently exposed to P. gingivalis for 15 weeks. Using protein-protein interaction (PPI) networks and Ingenuity Pathway Analysis (IPA), we identified hub genes, major biological processes, upstream regulators and genes potentially involved in tumor initiation and progression. We also validated gene expression and demonstrated genetic alteration of hub genes from clinical samples of head and neck cancer. Overall, we utilized bioinformatical methods to identify IL6, STAT1, LYN, BDNF, C3, CD274, PDCD1LG2, and CXCL10 as potential candidate genes that might facilitate the prevention and treatment of oral squamous cell carcinoma (OSCC), the most common type of head and neck squamous cell carcinoma (HNSCC).
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- 2019
114. Lignans from the Whole Plants of Hedyotis uncinella
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Yaping, Pan, Yan, Zhang, Chao, Fan, Yu, Zhang, Shikai, Yan, Huizi, Jin, and Weidong, Zhang
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Molecular Structure ,Hedyotis ,Lignans - Abstract
Two new lignans, named (7R, 8S)-balanophonin (1) and (7R, 8S)-tomentosanan A (2), together with eight known lignans, burselignan (3), (+)-isolariciresinol (4), (+)-lyoniresinol (5), 5-methoxy-(+)-isolariciresinol (6), (-)-syringaresinol (7), (+)-epipinoresinol (8), (-)-(7'S, 8S, 8'R)-4,4'-dihydroxy-3, 3', 5, 5'-. tetramethoxy-7', 9-epoxy-lignan-9'-ol-7-one (9), and (-)-(7R, 8S)-dihydrodehydrodiconiferyl alcohol (10) were isolated from the whole plants of Hedyotis uncinella Hook. et Am. Structures of these compounds were elucidated through 1H NMR, "C NMR, 2D NMR, ESI-MS and CD data.
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- 2019
115. Recombinant fimbriae protein of Porphyromonas gingivalis induces an inflammatory response via the TLR4/NF‑κB signaling pathway in human peripheral blood mononuclear cells
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Huanxu Guo, Yaping Pan, Yonggang Li, Jiangman Chen, Jing Cai, Xin Li, Wei Zhao, and Yibo Zhang
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Adult ,0301 basic medicine ,inflammatory cytokines ,medicine.medical_treatment ,Gene Expression ,P. gingivalis fimbriae ,Proinflammatory cytokine ,03 medical and health sciences ,0302 clinical medicine ,Gene expression ,Bacteroidaceae Infections ,Genetics ,medicine ,Humans ,Cloning, Molecular ,Porphyromonas gingivalis ,Inflammation ,Messenger RNA ,biology ,Chemistry ,NF-kappa B ,Articles ,General Medicine ,biochemical phenomena, metabolism, and nutrition ,biology.organism_classification ,Fusion protein ,Molecular biology ,Recombinant Proteins ,Toll-Like Receptor 4 ,cell proliferation ,030104 developmental biology ,Cytokine ,030220 oncology & carcinogenesis ,Leukocytes, Mononuclear ,TLR4 ,Cytokines ,bacteria ,toll-like receptor 4/nuclear factor kappa-light-chain-enhancer of activated B cells signaling pathway ,Tumor necrosis factor alpha ,Fimbriae Proteins ,Inflammation Mediators - Abstract
Porphyromonas gingivalis (P. gingivalis) is a periodontal pathogen that may accumulate with other organisms in subgingival plaque biofilms and is associated with periodontal disease. P. gingivalis fimbriae (FimA) is a filamentous structure on the surface of bacteria that is closely associated with bacterial adhesion to and colonization of host tissues, and serves an essential role in biofilm formation. The present study aimed to construct P. gingivalis FimA prokaryotic expression plasmids, purify a FimA fusion protein and explore the effect of a recombinant FimA protein on the inflammatory response in human peripheral blood mononuclear cells (PBMCs). P. gingivalis FimA prokaryotic expression plasmids were constructed by gene cloning and recombination technology. SDS-PAGE was used to evaluate the purified recombinant FimA protein. The cell proliferation rate and inflammatory cytokine expression of PBMCs treated with the FimA fusion protein with or without transfection with toll-like receptor 4 (TLR4) small interfering (si)RNA were detected by CCK-8 assays and ELISAs, respectively. The expression levels of TLR4, nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) and myeloid differentiation primary response 88 (MyD88) in PBMCs were detected by western blot analysis and reverse transcription quantitative polymerase chain reaction. A FimA fusion protein with high purity was obtained. FimA fusion protein treatment significantly increased PBMC proliferation and promoted the release of tumor necrosis factor-α (TNF-α), interleukin (IL)-6, matrix metalloproteinase (MMP)-8 and MMP-9 in PBMCs. TLR4 interference reversed the effects of the FimA fusion protein on PBMC proliferation and inflammatory cytokine release. The expression levels of TLR4, NF-κB and MyD88 in PBMCs were significantly increased following treatment with the FimA fusion protein, while the expression levels of these genes at the mRNA and protein levels decreased significantly in PBMCs following FimA fusion protein treatment and TLR4 interference. The FimA fusion protein increased PBMC proliferation and promoted the release of the inflammatory cytokines TNF-α, IL-6, MMP-8 and MMP-9 via the TLR4/NF-κB signaling pathway. FimA may serve as a promising therapeutic strategy for periodontal disease.
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- 2019
116. Porphyromonas gingivalis-Induced MIF Regulates Intercellular Adhesion Molecule-1 Expression in EA.hy926 Cells and Monocyte-Endothelial Cell Adhesion Through the Receptors CD74 and CXCR4
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Jingbo Liu, Wanyue Xu, Chen Li, Yaping Pan, Jingya Hou, Rong Li, Yun Wu, Xiaolin Tang, Li Lin, Yanqing Liu, and Dongmei Zhang
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0301 basic medicine ,Receptor complex ,Receptors, CXCR4 ,Immunology ,Intercellular Adhesion Molecule-1 ,Monocytes ,Cell Line ,03 medical and health sciences ,Chemokine receptor ,0302 clinical medicine ,medicine ,Cell Adhesion ,Immunology and Allergy ,Humans ,Cell adhesion ,Porphyromonas gingivalis ,Macrophage Migration-Inhibitory Factors ,biology ,Chemistry ,Monocyte ,Histocompatibility Antigens Class II ,Endothelial Cells ,intercellular cell adhesion molecule-1 ,biology.organism_classification ,Atherosclerosis ,Cell biology ,Endothelial stem cell ,Antigens, Differentiation, B-Lymphocyte ,Intramolecular Oxidoreductases ,030104 developmental biology ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,macrophage migration inhibitory factor ,Macrophage migration inhibitory factor ,Original Article ,CD74, CXCR4 - Abstract
Porphyromonas gingivalis (P. gingivalis) is an important pathogen that contributes to periodontal disease and causes infections that promote the progression of atherosclerosis. Our previous studies showed that macrophage migration inhibitory factor (MIF) facilitates monocyte adhesion to endothelial cells by regulating the expression of intercellular adhesion molecule-1 (ICAM-1) in P. gingivalis-infected endothelial cells. However, the detailed pathological role of MIF has yet to be elucidated in this context. To explore the functional receptor(s) of MIF that underlie its participation in the pathogenesis of atherosclerosis, we investigated the expression of the chemokine receptors CD74 and CXCR4 in endothelial cells, both of which were shown to be involved in the adhesion of monocytes to endothelial cells pretreated with P. gingivalis. Furthermore, the formation of a MIF, CD74, and CXCR4 ligand-receptor complex was revealed by our immunofluorescence staining and coimmunoprecipitation results. By interacting with the CD74/CXCR4 receptor complex, MIF may act as a crucial regulator of monocyte-endothelial cell adhesion and promote the atherosclerotic plaque formation induced by P. gingivalis.
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- 2018
117. Structural Basis of Ion Transport and Inhibition in Ferroportin
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Xiao Fan, Shuai Gao, Nieng Yan, Jiemin Shen, Yaping Pan, Ming Zhou, and Zhenning Ren
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biology ,Basis (linear algebra) ,Chemistry ,Ferroportin ,Biophysics ,biology.protein ,Ion transporter - Published
- 2021
118. Significance of maxillary sinus mucosal thickening in patients with periodontal disease
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Haijiao Zhao, Yaping Pan, Jingbo Liu, Qingxuan Wang, and Song Ren
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Adult ,Male ,Adolescent ,Gingival and periodontal pocket ,Maxillary sinus ,Alveolar Bone Loss ,Dentistry ,Mucous membrane of nose ,Cohort Studies ,Young Adult ,Sex Factors ,Risk Factors ,Periodontal Attachment Loss ,Humans ,Periodontal Pocket ,Medicine ,Child ,Periodontitis ,General Dentistry ,Dental alveolus ,Sinus (anatomy) ,Anatomy, Cross-Sectional ,Scientific Research Report ,business.industry ,Furcation Defects ,Smoking ,Age Factors ,Furcation defect ,Cone-Beam Computed Tomography ,Maxillary Sinus ,Middle Aged ,Maxillary Sinusitis ,medicine.disease ,Nasal Mucosa ,medicine.anatomical_structure ,Clinical attachment loss ,Female ,business - Abstract
Aim To characterise and measure the Schneiderian membranes of individuals with periodontal diseases in China and to analyse the factors impacting maxillary sinus mucosal thickness using cone-beam computed tomography (CBCT). Material and method A cohort of 221 patients with periodontal disease was subjected to cross-sectional CBCT examination. Various parameters, including age, sex, alveolar bone loss, furcation lesions and vertical infrabony pockets, were analysed as correlates of mucosal thickening (MT). Sinus mucosal thickness ≥2 mm qualified as MT. Results MT was detected in 103 (48.9%) patients, increasing in frequency as the degree of alveolar bone loss advanced (mild, 14.5%; moderate, 29.5%; severe, 87.9%). The association between MT and vertical infrabony pockets was statistically significant (P < 0.001). The likelihood of MT increased with moderate [odds ratio (OR) = 1.02] and severe (OR = 4.62) periodontal bone loss (P < 0.001), as well as with furcation lesions (OR = 2.76) and vertical infrabony pockets (OR = 13.58). Conclusions Relative to the case in patients with periodontitis and normal mucosa, the probability of MT increased dramatically as alveolar bone loss worsened. Periodontal pathologies (i.e. furcation lesions and vertical infrabony pockets) were also more likely to coincide with MT.
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- 2015
119. The expression of inducible nitric oxide synthase in the gingiva of rats with periodontitis and diabetes mellitus
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Yun Wu, Chunling Pan, Lu Yan, Junchao Liu, Dongmei Zhang, Yaping Pan, and Shangmin Sun
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Male ,0301 basic medicine ,medicine.medical_specialty ,Alveolar Bone Loss ,Gingiva ,Nitric Oxide Synthase Type II ,Bone resorption ,Rats, Sprague-Dawley ,03 medical and health sciences ,0302 clinical medicine ,Western blot ,Internal medicine ,Diabetes mellitus ,Diabetes Mellitus ,medicine ,Animals ,Periodontitis ,General Dentistry ,Dental alveolus ,biology ,medicine.diagnostic_test ,business.industry ,030206 dentistry ,Cell Biology ,General Medicine ,medicine.disease ,Rats ,Resorption ,Nitric oxide synthase ,030104 developmental biology ,Endocrinology ,Otorhinolaryngology ,biology.protein ,Immunohistochemistry ,business - Abstract
To ascertain the role of inducible nitric oxide synthase (iNOS) in the periodontitis response during diabetes.Twenty-four male SD rats were randomly divided into four groups: control group (Control), diabetes mellitus group (D), diabetes mellitus plus periodontitis group (DP), and periodontitis group (P). Periodontitis and diabetes were established separately. Then the gingival tissue and alveolar bone were collected. A stereomicroscope was used to evaluate bone loss. The expression of iNOS, TNF-α, and NF-κB in the gingiva was detected by immunohistochemical staining, real-time PCR, and western blot analysis.Significant bone loss was observed in the DP and P groups and more extensive bone resorption was discovered in the DP group than in the P group (P0.05). The immunohistochemical staining analysis revealed enhanced expression of iNOS located in the gingiva of the three disease groups compared with the control group (P0.05). In particular, the level of iNOS was significantly higher in the DP group than in the P group (P0.05). This elevated trend of iNOS was further demonstrated by quantitative PCR and western blot analysis. Similarly, the mRNA and protein expression levels of NF-κB in the D, DP, and P groups were significantly higher than those of the control group, as was the level of TNF-α protein (P0.05).Our results proved diabetes exacerbated alveolar bone resorption in a periodontitis rat model. iNOS may be the inflammatory mediator in the course of periodontal injury promoted by diabetes.
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- 2020
120. Mechanism of Ion Permeation in the Eukaryotic Copper Transporter CTR
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Yaping Pan, Ming Zhou, and Kehan Chen
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Ion permeation ,chemistry ,Biophysics ,chemistry.chemical_element ,Transporter ,Copper ,Mechanism (sociology) - Published
- 2020
121. A long-lived Donor–Acceptor fluorescent probe for sequential detection of Cu2+ and biothiols
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Duobin Chao, Yaping Pan, and Xue-Wang Gao
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inorganic chemicals ,Detection limit ,Absorption (pharmacology) ,Fluorophore ,Chemistry ,02 engineering and technology ,Glutathione ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Photochemistry ,01 natural sciences ,Fluorescence ,Atomic and Molecular Physics, and Optics ,0104 chemical sciences ,Analytical Chemistry ,chemistry.chemical_compound ,Titration ,0210 nano-technology ,Donor acceptor ,Instrumentation ,Spectroscopy - Abstract
A new long-lived Donor–Acceptor (D–A) fluorophore based on carbazolyl dicyanobenzene was developed as an ON–OFF–ON multifunctional fluorescent probe 1 for sequential detection of Cu2+ and biothiols (Cys, Hcy and GSH). The fluorescence of probe 1 can be significantly and selectively quenched by Cu2+. Meanwhile, the fluorescence lifetime decreased from 2.1 μs to 18.5 ns. The limit of detection was determined to be 33.6 nM. Upon addition of biothiols (Cys, Hcy and GSH), the generated ensemble 1-Cu2+ displayed a “turn-on” fluorescent response at 555 nm and an obvious recovery in fluorescence lifetime and UV–vis absorption within 1 min. The limit of detection for Cys, Hcy and GSH were calculated by fluorescence titration experiments to be 0.19, 0.21 and 0.29 μM, respectively. The ensemble 1-Cu2+ was further successfully applied in bioimaging.
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- 2020
122. Meeting report: a close look at oral biofilms and microbiomes
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Xin Xu, Ma Lvyan, Yaping Pan, Denis F. Kinane, Syngcuk Kim, Zhengwei Huang, Li Chen, Feng Chen, Jian Xu, Hyun Koo, and Xuedong Zhou
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0301 basic medicine ,Medical education ,030106 microbiology ,West china ,Comment ,biochemical phenomena, metabolism, and nutrition ,Pathogenicity ,lcsh:RK1-715 ,03 medical and health sciences ,stomatognathic diseases ,Beijing ,lcsh:Dentistry ,Political science ,Microbiome ,Oral Microbiome ,General Dentistry - Abstract
The “Biofilms, Microbiomes and Oral Diseases: Challenges and Future Perspectives” symposium jointly organized by Penn Dental Medicine and West China School of Stomatology was held on 30 September 2017 at Penn Wharton China Center (PWCC) in Beijing, China. The topics included the pathogenicity of oral biofilms, novel strategies for the control of biofilm-related diseases, oral microbiome and single-cell approaches, and the link between oral diseases and overall health. Researchers from a number of disciplines, representing institutions from China and Penn Dental Medicine, gathered to discuss advances in our understanding of biofilms, as well as future directions for the control of biofilm-related oral and systemic diseases.
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- 2018
123. The prevalence rate of periodontal pathogens and its association with oral squamous cell carcinoma
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Yuchao Li, Fengxue Geng, Yaping Pan, Xiaoting Shi, Xue Zhang, Chunrong Chang, and Xida Zhao
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Adult ,Male ,Pathology ,medicine.medical_specialty ,Dental Plaque ,Applied Microbiology and Biotechnology ,Periodontal pathogen ,Metastasis ,03 medical and health sciences ,RNA, Ribosomal, 16S ,Medicine ,Humans ,Periodontal Pocket ,Porphyromonas gingivalis ,In Situ Hybridization, Fluorescence ,030304 developmental biology ,Aged ,0303 health sciences ,biology ,Fusobacterium nucleatum ,030306 microbiology ,business.industry ,Mouth Mucosa ,Cancer ,General Medicine ,Middle Aged ,biology.organism_classification ,medicine.disease ,Squamous carcinoma ,stomatognathic diseases ,Streptococcus sanguinis ,Clinical attachment loss ,Carcinoma, Squamous Cell ,Female ,Mouth Neoplasms ,Streptococcus sanguis ,business ,Biotechnology - Abstract
Mounting evidence suggests a causal relationship between specific bacterial infections or microbial compositions and the development of certain malignant neoplasms. In this study, we performed research through 16S rRNA amplicon sequencing, qPCR and fluorescence in situ hybridization to certify the relationship between periodontal pathogens and oral squamous cell carcinoma (OSCC). Subgingival plaque, cancer and paracancerous tissues from 6 patients with OSCC were selected for mapping bacterial profiles by 16S rRNA amplicon sequencing. The research showed that periodontal pathogens were enriched in cancer and paracancerous tissues, while the bacterial profiles were similar between the cancer tissues and subgingival plaque. Furthermore, the relative abundance of Porphyromonas gingivalis, Fusobacterium nucleatum and Streptococcus sanguinis was detected in 61 cancer tissues, paracancerous tissues and subgingival plaque samples and in 30 normal tissues by qPCR. The results revealed that P. gingivalis and F. nucleatum existed at higher levels in cancer tissue than in normal tissues and were correlated with subgingival plaques. P. gingivalis was detected using a special oligonucleotide probe in 60.7% of OSCC tissues, 32.8% of paracancerous tissues and 13.3% of normal tissues. Relevance analysis showed that P. gingivalis infection was positively associated with late clinical staging, low differentiation and lymph node metastasis in patients with OSCC, which was accompanied by deeper periodontal pockets, severe clinical attachment loss and loss of teeth. This study revealed that there might be a close relationship between oral microorganisms, particularly periodontal pathogens, and OSCC, which might enrich the pathogenesis of oral squamous carcinoma.
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- 2018
124. Effect of platelet-rich fibrin on alveolar ridge preservation: A systematic review
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Jiayu, Pan, Quifang, Xu, Jingya, Hou, Yun, Wu, Yanqing, Liu, Rong, Li, Yaping, Pan, and Dongmei, Zhang
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Wound Healing ,Platelet-Rich Fibrin ,Tooth Extraction ,Alveolar Process ,Tooth Socket - Abstract
Platelet-rich fibrin (PRF) is the second generation of platelet concentrates and is used in many areas of dentistry. However, whether PRF is effective for alveolar ridge preservation remains controversial. The authors conducted research to evaluate the potential of PRF to preserve the alveolar ridge.A comprehensive literature search was conducted in MEDLINE, Cochrane Central Register of Controlled Trials, and Embase. Only randomized controlled trials were included. A systematic review was made for postoperative pain, soft-tissue healing, bone density, horizontal and vertical ridge dimension changes, and histologic analysis. The meta-analysis was performed on the alveolar osteitis, mesial and distal bone height changes, and bone fill with Review Manager Version 5.3 software.Among the 588 eligible articles found in the initial search, 7 published studies from 2012 through 2019 were included. The authors' qualitative analysis showed that PRF may play a positive role in reducing postoperative pain and ridge dimension changes after tooth extraction. Among the 7 articles, only 2 trials assessed the effect of PRF on the alveolar osteitis, mesial and distal bone height changes, and bone fill. Results of our meta-analysis showed that smaller mesial bone height changes (standard mean difference, -1.07; 95% confidence interval, -1.92 to 0.22) and a greater percentage of bone fill (standard mean difference, 0.82; 95% confidence interval, 0.32 to 1.33) were observed in the PRF group.Given the potential value of PRF, consideration should be given to PRF after tooth extraction. However, more high-quality trials are necessary to evaluate the exact role of PRF.Based on the authors' results, the usage of PRF was suggested in alveolar ridge preservation.
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- 2018
125. Porphyromonas gingivalis ATCC 33277 promotes intercellular adhesion molecule-1 expression in endothelial cells and monocyte-endothelial cell adhesion through macrophage migration inhibitory factor
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Qiu-fang Xu, Yaping Pan, Wanyue Xu, Chen Li, Jingbo Liu, Li Lin, Yun Wu, Xiaolin Tang, Dongmei Zhang, Jiayu Pan, and Jingya Hou
- Subjects
0301 basic medicine ,Microbiology (medical) ,Cell Survival ,THP-1 Cells ,Endothelial cells ,medicine.medical_treatment ,Intercellular Adhesion Molecule-1 ,lcsh:QR1-502 ,030204 cardiovascular system & hematology ,Microbiology ,lcsh:Microbiology ,Monocytes ,03 medical and health sciences ,0302 clinical medicine ,Cell Adhesion ,medicine ,Humans ,Intercellular cell adhesion molecule-1 ,Periodontitis ,Cell adhesion ,Macrophage Migration-Inhibitory Factors ,Porphyromonas gingivalis ,Macrophage migration inhibitory factor ,biology ,Monocyte ,biology.organism_classification ,Intercellular adhesion molecule ,Intramolecular Oxidoreductases ,Endothelial stem cell ,030104 developmental biology ,Cytokine ,medicine.anatomical_structure ,Cytokines ,Research Article - Abstract
Background Porphyromonas gingivalis (P. gingivalis), one of the main pathogenic bacteria involved in periodontitis, induces the expression of intercellular adhesion molecule − 1 (ICAM-1) and monocyte-endothelial cell adhesion. This effect plays a pivotal role in atherosclerosis development. Macrophage migration inhibitory factor (MIF) is a multifunctional cytokine and critically affects atherosclerosis pathogenesis. In this study, we tested the involvement of MIF in the P. gingivalis ATCC 33277-enhanced adhesive properties of endothelial cells. Results Endothelial MIF expression was enhanced by P. gingivalis ATCC 33277 infection. The MIF inhibitor ISO-1 inhibited ICAM-1 production in endothelial cells, and monocyte-endothelial cell adhesion was induced by P. gingivalis ATCC 33277 infection. However, the addition of exogenous human recombinant MIF to P. gingivalis ATCC 33277-infected endothelial cells facilitated monocyte recruitment by promoting ICAM-1 expression in endothelial cells. Conclusions These experiments revealed that MIF in endothelial cells participates in the pro-atherosclerotic lesion formation caused by P. gingivalis ATCC 33277 infection. Our novel findings identify a more detailed pathological role of P. gingivalis ATCC 33277 in atherosclerosis.
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- 2018
126. The Effects of Antimicrobial Peptide Nal-P-113 on Inhibiting Periodontal Pathogens and Improving Periodontal Status
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Yaping Pan, Li Lin, Lisi Ai, Dongmei Zhang, Yu Zhang, Chen Li, Hongyan Wang, Jya-Wei Cheng, and Hui-Yuan Yu
- Subjects
0301 basic medicine ,Adult ,Male ,Article Subject ,Gingiva ,lcsh:Medicine ,General Biochemistry, Genetics and Molecular Biology ,Microbiology ,03 medical and health sciences ,0302 clinical medicine ,Medicine ,Aggressive periodontitis ,Humans ,Periodontal Pocket ,Porphyromonas gingivalis ,Dental alveolus ,Periodontal Diseases ,Inflammation ,General Immunology and Microbiology ,biology ,Fusobacterium nucleatum ,business.industry ,lcsh:R ,Biofilm ,Streptococcus gordonii ,Treponema denticola ,030206 dentistry ,General Medicine ,Middle Aged ,biology.organism_classification ,medicine.disease ,Chronic periodontitis ,Anti-Bacterial Agents ,030104 developmental biology ,Biofilms ,Clinical Study ,beta-Alanine ,Female ,business ,Peptides - Abstract
Periodontal disease consists of chronic gingival inflammation characterized by both degradation of the periodontal connective tissue and alveolar bone loss. Drug therapy is used as an auxiliary treatment method in severe chronic periodontitis, aggressive periodontitis, and periodontitis-associated systemic disease. Nal-P-113, a modified antimicrobial peptide, specifically replaces the histidine residues of P-113 with the bulky amino acidβ-naphthylalanine, and our previous studies have verified that this novel peptide is not toxic to the human body within a certain concentration range. The objective of the present study was to evaluate the effect of Nal-P-113 on periodontal pathogens and periodontal status in clinical studies. In a split-mouth clinical trial, the pocket depth and bleeding index values tended to decrease in the experimental group compared with those in the control group. SEM results verified that Nal-P-113 restrained the maturation of plaque. Based on real-time polymerase chain reaction, the levels ofFusobacterium nucleatum,Streptococcus gordonii,Treponema denticola,andPorphyromonas gingivalisin subgingival plaque were decreased when the subjects were given Nal-P-113. Bacterial growth curve analysis and a biofilm susceptibility assay verified that Nal-P-113 at a concentration of 20 μg/mL restrained the growth ofS. gordonii,F. nucleatum,andP. gingivalisand biofilm formation. Therefore, Nal-P-113 effectively reduces periodontal pathogens and ameliorates periodontal status.
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- 2018
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127. Relationship among clinical periodontal, microbiologic parameters and lung function in participants with chronic obstructive pulmonary disease
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Hongyan Wang, Lisi Tan, Xiaolin Tang, Chunling Pan, and Yaping Pan
- Subjects
0301 basic medicine ,medicine.medical_specialty ,Oral Hygiene Index ,medicine.disease_cause ,Oral hygiene ,03 medical and health sciences ,Pulmonary Disease, Chronic Obstructive ,0302 clinical medicine ,Internal medicine ,Forced Expiratory Volume ,Periodontal Attachment Loss ,medicine ,Humans ,Respiratory system ,Porphyromonas gingivalis ,Periodontitis ,COPD ,biology ,business.industry ,Streptococcus ,030206 dentistry ,medicine.disease ,biology.organism_classification ,respiratory tract diseases ,Respiratory Function Tests ,Pneumonia ,030104 developmental biology ,Periodontics ,Klebsiella pneumonia ,business - Abstract
BACKGROUND The aim of this study was to evaluate the relationship among clinical periodontal, microbiologic parameters and lung function in participants with chronic obstructive pulmonary disease (COPD). METHODS A total of 160 participants were recruited, including 80 participants with COPD (COPD group) and 80 participants without COPD (control group). All participants completed questionnaires and underwent clinical periodontal and lung function examinations. Subgingival plaques were obtained to determine the prevalence of selected oral and respiratory bacterial species. RESULTS 1) Significant relationships were noted in the participants among oral hygiene index-simplified (OHI-S), clinical attachment level (CAL) and forced expiratory volume in one second (FEV1%). 2) Porphyromonas gingivalis (Pg), Klebsiella pneumonia (Kp), Pseudomonas aeruginosa (Pa) and Streptococcus pneumonia (Sp) prevalence was increased in participants with COPD compared with control participants. 3) A significant negative association was noted between the relative content of Pg and FEV1% in participants with COPD. CONCLUSION The results of this study confirm that periodontal destruction and oral pathogens are associated with lung function.
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- 2017
128. Efficacy of a novel antimicrobial peptide against periodontal pathogens in both planktonic and polymicrobial biofilm states
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Li Lin, Yaping Pan, Ya-Han Chih, Hongyan Wang, Hui-Yuan Yu, and Jya-Wei Cheng
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DNA, Bacterial ,Serum ,Periodontal Ligament ,Antimicrobial peptides ,Gingiva ,Biomedical Engineering ,Microbial Sensitivity Tests ,Penicillins ,Buffers ,Dental plaque ,Biochemistry ,Microbiology ,Biomaterials ,Anti-Infective Agents ,Metronidazole ,medicine ,Animals ,Humans ,Saliva ,Molecular Biology ,Porphyromonas gingivalis ,bcl-2-Associated X Protein ,Mouth ,Bacteria ,Cell Death ,biology ,Cell Membrane ,Chlorhexidine ,Streptococcus gordonii ,Biofilm ,General Medicine ,Plankton ,Antimicrobial ,biology.organism_classification ,medicine.disease ,Caspase 9 ,Rats ,Biofilms ,Cattle ,Fusobacterium nucleatum ,Peptides ,Biotechnology - Abstract
Streptococcus gordonii, Fusobacterium nucleatum and Porphyromonas gingivalis represent the early, middle and late colonizers of the bacterial accretion in dental plaque biofilms. These sessile communities constitute a protected mode of growth that promotes survival in a hostile environment. This study describes a novel and unrecognized role for a synthetic cationic antimicrobial peptide, Nal-P-113, which inhibits and kills periodontal bacteria in planktonic state, inhibits the formation of biofilms and eradicates polymicrobial biofilms. Nal-P-113 is also stable in saliva, serum and saline solution. At a concentration less than 320 μg/mL which is harmless to normal oral cells, Nal-P-113 can kill bacteria in planktonic state. At a concentration of antimicrobial peptide Nal-P-113 (1280 μg/mL) which only causes slight damages to normal oral cells is needed to kill bacteria in biofilm state. It is worth mentioning that this concentration of Nal-P-113 is harmless to rat oral mucosa compared to chlorhexidine. The mechanism of Nal-P-113 inhibiting and killing periodontal bacteria might rely on the abilities to permeabilize and/or to form pores within the cytoplasmic membranes, thus causes the death of bacteria. Here, we provided a novel and stable antimicrobial peptide with very low mammalian cytotoxicity, which can inhibit and kill periodontal bacteria in both planktonic and polymicrobial biofilm states. Statement of Significance Nal-P-113 is a potent antimicrobial peptide with strong antimicrobial ability, improved deficiency compared with other antibacterial peptides, and remains stable in phosphate buffered saline, saliva, brain-heart infusion medium and bovine calf serum. Nal-P-113 exhibits a broad spectrum of bacteriocidal activity with excellent eradicating capability on oral pathogens and the respective biofilms. In this study, we used propidium iodide staining, scanning electron microscopy and transmission electron microscopy to confirm that Nal-P-113 can perforate plasmalemma thereby resulting in the death of oral pathogens and disintegrate the respective biofilms. Nal-P-113 also showed effective anti-plaque biofilms and cytotoxicity in the rat periodontitis model. No adverse effects can be observed on the gingivomucosa tissue. In short, the antimicrobial peptide Nal-P-113 presented to be an effective yet have low mammalian cytotoxicity agent with potential application in the clinic. This study provides a proof of concept in applying antimicrobial peptides in the clinical perspective.
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- 2015
129. Porphyromonas gingivalis promotes the cell cycle and inflammatory cytokine production in periodontal ligament fibroblasts
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Qian Li, Xiaolin Tang, Yaping Pan, Fengxue Geng, Chen Li, Chunling Pan, and Junchao Liu
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Cyclin-Dependent Kinase Inhibitor p21 ,Cyclin E ,Periodontal Ligament ,medicine.medical_treatment ,Blotting, Western ,Enzyme-Linked Immunosorbent Assay ,Inflammation ,Biology ,Cyclin D1 ,medicine ,Humans ,Periodontal fiber ,RNA, Messenger ,General Dentistry ,Porphyromonas gingivalis ,Cells, Cultured ,Cell Proliferation ,Reverse Transcriptase Polymerase Chain Reaction ,Cell growth ,Cell Cycle ,Cell Biology ,General Medicine ,Fibroblasts ,Cell cycle ,Flow Cytometry ,biology.organism_classification ,Up-Regulation ,Cytokine ,Otorhinolaryngology ,Immunology ,medicine.symptom - Abstract
Objective The infection of Porphyromonas gingivalis ( P. gingivalis ) modulates host immune-inflammatory responses and destructs homeostasis of normal cell cycle, thereby leading to periodontal tissue destruction. Human periodontal ligament fibroblasts (PDLFs) are key players in the host immune responses and periodontal tissue regeneration. The aim of the present study was to discover the effects of P. gingivalis infection on the cell cycle and inflammatory cytokine production in PDLFs. Design P. gingivalis infection model into PDLFs was established. The effect of P. gingivalis on the cell proliferation and cell cycle were detected by MTT and flow cytometry. The p21 , cyclin D1 and cyclin E mRNA expression, p21 protein expression, as well as IL-6 and IL-8 protein levels were analyzed by RT-qPCR, Western blot and ELISA, respectively. Results P. gingivalis promoted proliferation and G1 phase of PDLFs. G1 phase promotion was associated with the decreased level of p21 and the up-regulation of cyclin D1 at 6 h, and with the increased level of cyclin E at 12 h. Simultaneously, the immune-inflammatory response of PDLFs was initiated by P. gingivalis during the initial stage of infection, including the increased expressions of IL-6 and IL-8. Conclusion We confirmed that the infection of P. gingivalis could modulate the expression of PDLF genes, which control cell cycle and inflammatory cytokine production. Thus, P. gingivalis may contribute to the proliferation and inflammation of periodontal tissue.
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- 2015
130. Risk factors of chronic periodontitis on healing response: a multilevel modelling analysis
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C. Pan, J. Song, J. Liu, C. Li, Yaping Pan, and H. Zhao
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Adult ,Male ,Multivariate statistics ,medicine.medical_specialty ,China ,Dentistry ,Health Informatics ,Disease ,lcsh:Computer applications to medicine. Medical informatics ,Models, Biological ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,Medicine ,Humans ,030212 general & internal medicine ,Periodontitis ,Wound Healing ,business.industry ,Health Policy ,Multilevel modelling ,Significant difference ,030206 dentistry ,Middle Aged ,medicine.disease ,Prognosis ,Chronic periodontitis ,Computer Science Applications ,Clinical attachment loss ,Risk factors ,Chronic Periodontitis ,Multilevel Analysis ,Variance components ,lcsh:R858-859.7 ,Female ,Periodontal Index ,business ,Research Article - Abstract
Background Chronic periodontitis is a multifactorial polygenetic disease with an increasing number of associated factors that have been identified over recent decades. Longitudinal epidemiologic studies have demonstrated that the risk factors were related to the progression of the disease. A traditional multivariate regression model was used to find risk factors associated with chronic periodontitis. However, the approach requirement of standard statistical procedures demands individual independence. Multilevel modelling (MLM) data analysis has widely been used in recent years, regarding thorough hierarchical structuring of the data, decomposing the error terms into different levels, and providing a new analytic method and framework for solving this problem. The purpose of our study is to investigate the relationship of clinical periodontal index and the risk factors in chronic periodontitis through MLM analysis and to identify high-risk individuals in the clinical setting. Methods Fifty-four patients with moderate to severe periodontitis were included. They were treated by means of non-surgical periodontal therapy, and then made follow-up visits regularly at 3, 6, and 12 months after therapy. Each patient answered a questionnaire survey and underwent measurement of clinical periodontal parameters. Results Compared with baseline, probing depth (PD) and clinical attachment loss (CAL) improved significantly after non-surgical periodontal therapy with regular follow-up visits at 3, 6, and 12 months after therapy. The null model and variance component models with no independent variables included were initially obtained to investigate the variance of the PD and CAL reductions across all three levels, and they showed a statistically significant difference (P
- Published
- 2017
131. Persistent Exposure to Porphyromonas gingivalis Promotes Proliferative and Invasion Capabilities, and Tumorigenic Properties of Human Immortalized Oral Epithelial Cells
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Hongyan Wang, Hongyang Wang, Haijiao Zhao, Yan Guo, Yaping Pan, Chen Li, Fengxue Geng, and Junchao Liu
- Subjects
0301 basic medicine ,Microbiology (medical) ,Cell ,Immunology ,long-term infection ,Biology ,Microbiology ,03 medical and health sciences ,0302 clinical medicine ,Multiplicity of infection ,Immune system ,medicine ,inflammatory microenvironment ,Porphyromonas gingivalis ,Periodontitis ,Cell growth ,tumorigenic properties ,Cell cycle ,medicine.disease ,biology.organism_classification ,030104 developmental biology ,medicine.anatomical_structure ,Infectious Diseases ,Cell culture ,030220 oncology & carcinogenesis ,OSCC - Abstract
Recent epidemiological studies revealed a significant association between oral squamous cell carcinoma (OSCC) and Porphyromonas gingivalis, a major pathogen of periodontal disease. As a keystone pathogen of periodontitis, P. gingivalis is known not only to damage local periodontal tissues, but also to evade the host immune system and eventually affect systemic health. However, its role in OSCC has yet to be defined. To explore the underlying effect of chronic P. gingivalis infection on OSCC and to identify relevant biomarkers as promising targets for therapy and prevention, we established a novel model by exposing human immortalized oral epithelial cells (HIOECs) to P. gingivalis at a low multiplicity of infection (MOI) for 5-23 weeks. The P. gingivalis infected HIOECs were monitored for tumor biological alteration by proliferation, wound healing, transwell invasion, and gelatin zymography assays. Microarray and proteomic analyses were performed on HIOECs infected with P. gingivalis for 15 weeks, and some selected data were validated by quantitative real-time PCR and (or) western blot on cells infected for 15 and 23 weeks. Persistent exposure to P. gingivalis caused cell morphological changes, increased proliferation ability with higher S phase fraction in the cell cycle, and promoted cell migratory and invasive properties. In combining results of bioinformatics analyses and validation assays, tumor-related genes such as NNMT, FLI1, GAS6, lncRNA CCAT1, PDCD1LG2, and CD274 may be considered as the key regulators in tumor-like transformation in response to long-time exposure of P. gingivalis. In addition, some useful clinical biomarkers and novel proteins were also presented. In conclusion, P. gingivalis could promote tumorigenic properties of HIOECs, indicating that chronic P. gingivalis infection may be considered as a potential risk factor for oral cancer. The key regulators detected from the present model might be used in monitoring the development of OSCC with chronic periodontal infection.
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- 2017
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132. Molecular pathways underlying inhibitory effect of antimicrobial peptide Nal-P-113 on bacteria biofilms formation of Porphyromonas gingivalis W83 by DNA microarray
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Hongyan Wang, Yaping Pan, Li Lin, Jya-Wei Cheng, Hui-Yuan Yu, and Lisi Tan
- Subjects
0301 basic medicine ,Microbiology (medical) ,medicine.drug_class ,030106 microbiology ,Antibiotics ,Antimicrobial peptides ,Microbial Sensitivity Tests ,Microarray ,Biology ,Microbiology ,03 medical and health sciences ,Anti-Infective Agents ,Bacterial Proteins ,medicine ,Periodontitis ,Porphyromonas gingivalis ,Nal-P-113 ,Oligonucleotide Array Sequence Analysis ,Mouth ,Gene Expression Profiling ,Biofilm ,Porphyromonas gingivalis W83 ,Glycosyltransferases ,Gene Expression Regulation, Bacterial ,Hydrogen Peroxide ,Antimicrobial ,biology.organism_classification ,Gene expression profiling ,030104 developmental biology ,Biofilms ,Microscopy, Electron, Scanning ,DNA microarray ,Carrier Proteins ,Bacteria ,Research Article - Abstract
Background Wound-related infection remains a major challenge for health professionals. One disadvantage in conventional antibiotics is their inability to penetrate biofilms, the main protective strategy for bacteria to evade irradiation. Previously, we have shown that synthetic antimicrobial peptides could inhibit bacterial biofilms formation. Results In this study, we first delineated how Nal-P-113, a novel antimicrobial peptide, exerted its inhibitory effects on Porphyromonas gingivalis W83 biofilms formation at a low concentration. Secondly, we performed gene expression profiling and validated that Nal-P-113 at a low dose significantly down-regulated genes related to mobile and extrachromosomal element functions, transport and binding proteins in Porphyromonas gingivalis W83. Conclusions These findings suggest that Nal-P-113 at low dose is sufficient to inhibit the formation of biofilms although Porphyromonas gingivalis W83 may maintain its survival in the oral cavity. The newly discovered molecular pathways may add the knowledge of developing a new strategy to target bacterial infections in combination with current first-line treatment in periodontitis. Electronic supplementary material The online version of this article (doi:10.1186/s12866-017-0948-z) contains supplementary material, which is available to authorized users.
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- 2017
133. NLRP3 inflammasome is required for apoptosis of Aggregatibacter actinomycetemcomitans-infected human osteoblastic MG63 cells
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Yaping Pan, Panyu Zhao, Junchao Liu, and Chunling Pan
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Small interfering RNA ,Histology ,Inflammasomes ,Apoptosis ,Biology ,Aggregatibacter actinomycetemcomitans ,Cell Line ,Microbiology ,Pathogenesis ,NLR Family, Pyrin Domain-Containing 3 Protein ,medicine ,Humans ,Aggressive periodontitis ,Secretion ,Gene knockdown ,Osteoblasts ,Inflammasome ,Cell Biology ,General Medicine ,medicine.disease ,biology.organism_classification ,Pasteurellaceae Infections ,Carrier Proteins ,medicine.drug - Abstract
Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) is a Gram-negative bacterium which is implicated in the pathogenesis of human periodontal disease and in particular aggressive periodontitis. Virulence factors from A. actinomycetemcomitans have been shown to induce apoptosis of osteoblasts, however, the underlying mechanisms of the induction of apoptosis are poorly understood. In the present study, the infection of A. actinomycetemcomitans in human osteoblastic MG63 cells was established. Accordingly, A. actinomycetemcomitans infection enhanced significant apoptosis of MG63 cells. We found that both expression levels of NLRP3 and ASC were increased dramatically after MG63 cell cultures exposed to A. actinomycetemcomitans. Moreover, the secretion of mature interleukin-1β (IL-1β) and IL-18 were extensively induced in A. actinomycetemcomitans-infected cells as compared with non-invasion group of MG63 cell cultures, indicating the activation of the NLRP3 inflammasome during infection. Finally, we found that the knockdown expression of NLRP3 by specific small interfering RNA (siRNA) attenuated apoptosis of A. actinomycetemcomitans-infected MG63 cells. Our data suggest that A. actinomycetemcomitans promotes apoptosis of human osteoblasts at least partially through the NLRP3 inflammasome.
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- 2014
134. 16S rDNA-based metagenomic analysis of dental plaque and lung bacteria in patients with severe acute exacerbations of chronic obstructive pulmonary disease
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Yaping Pan, Chi Yuan Li, Lisi Tan, and Hong Wang
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Acinetobacter baumannii ,Adult ,DNA, Bacterial ,Male ,Dental Plaque ,Dental plaque ,medicine.disease_cause ,Aggregatibacter actinomycetemcomitans ,DNA, Ribosomal ,Microbiology ,Pulmonary Disease, Chronic Obstructive ,RNA, Ribosomal, 16S ,Intensive care ,medicine ,Bacteroides ,Humans ,Tannerella forsythia ,Prospective Studies ,Lung ,Porphyromonas gingivalis ,Aged ,Aged, 80 and over ,biology ,Smoking ,Pathogenic bacteria ,Treponema denticola ,Middle Aged ,biology.organism_classification ,medicine.disease ,Bacterial Load ,Trachea ,Klebsiella pneumoniae ,stomatognathic diseases ,Streptococcus pneumoniae ,Biofilms ,Pseudomonas aeruginosa ,Periodontics ,Female ,Metagenomics ,Capnocytophaga - Abstract
Background and Objective Acute exacerbations of chronic obstructive pulmonary disease (AE-COPD) are leading causes of mortality in hospital intensive care units. We sought to determine whether dental plaque biofilms might harbor pathogenic bacteria that can eventually cause lung infections in patients with severe AE-COPD. Material and Methods Paired samples of subgingival plaque biofilm and tracheal aspirate were collected from 53 patients with severe AE-COPD. Total bacterial DNA was extracted from each sample individually for polymerase chain reaction amplification and/or generation of bacterial 16S rDNA sequences and cDNA libraries. We used a metagenomic approach, based on bacterial 16S rDNA sequences, to compare the distribution of species present in dental plaque and lung. Results Analysis of 1060 sequences (20 clones per patient) revealed a wide range of aerobic, anaerobic, pathogenic, opportunistic, novel and uncultivable bacterial species. Species indistinguishable between the paired subgingival plaque and tracheal aspirate samples (97–100% similarity in 16S rDNA sequence) were dental plaque pathogens (Aggregatibacter actinomycetemcomitans, Capnocytophaga sputigena, Porphyromonas gingivalis, Tannerella forsythia and Treponema denticola) and lung pathogens (Acinetobacter baumannii, Klebsiella pneumoniae, Pseudomonas aeruginosa and Streptococcus pneumoniae). Real-time polymerase chain reaction of 16S rDNA indicated lower levels of Pseudomonas aeruginosa and Porphyromonas gingivalis colonizing the dental plaques compared with the paired tracheal aspirate samples. Conclusion These results support the hypothesis that dental bacteria may contribute to the pathology of severe AE-COPD.
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- 2014
135. Porphyromonas gingivalis modulates Pseudomonas aeruginosa-induced apoptosis of respiratory epithelial cells through the STAT3 signaling pathway
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Frank A. Scannapieco, Chunling Pan, Di Teng, Qian Li, Li Lin, Yaping Pan, Yurong Kou, and Elaine M. Haase
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STAT3 Transcription Factor ,Programmed cell death ,Survivin ,Immunology ,Apoptosis ,Respiratory Mucosa ,Biology ,medicine.disease_cause ,Microbiology ,Stat3 Signaling Pathway ,Inhibitor of Apoptosis Proteins ,Cell Line, Tumor ,medicine ,Humans ,Porphyromonas gingivalis ,Caspase 3 ,Pseudomonas aeruginosa ,Respiratory infection ,Epithelial Cells ,biology.organism_classification ,Phenotype ,Infectious Diseases ,Proto-Oncogene Proteins c-bcl-2 ,bcl-Associated Death Protein ,Signal transduction ,Signal Transduction - Abstract
Pseudomonas aeruginosa is an important opportunistic bacterial pathogen, causing infections of respiratory and other organ systems in immunocompromised hosts that may invade and proliferate in mucosal epithelial cells to induce apoptosis. Previous studies suggest that oral bacteria, especially gram-negative periodontal pathogens, may enhance P. aeruginosa invasion into respiratory epithelial cells to augment tissue destruction. In this study, we investigated the effect of the periodontopathogen Porphyromonas gingivalis on P. aeruginosa-induced epithelial cell apoptosis. P. gingivalis invasion transiently inhibited P. aeruginosa-induced apoptosis in respiratory epithelial cells via the signal transducer and activator of transcription 3 (STAT3) signaling pathway. The activated STAT3 up-regulated the downstream anti-apoptotic moleculars survivin and B-cell leukemia-2 (bcl-2). This process was accompanied by down-regulation of pro-apoptosis molecular Bcl-2-associated death promoter (bad) and caspase-3 activity inhibition. In addition, the activation of the STAT3 pathway was affected by P. gingivalis in a dose-dependent manner. Finally, co-invasion of P. aeruginosa and P. gingivalis led to greater cell death compared with P. aeruginosa challenge alone. These results suggest that regulation of P. aeruginosa-induced apoptosis by P. gingivalis contributes to the pathogenesis of respiratory disease. Interference with this process may provide a potential therapeutic strategy for the treatment and prevention of respiratory disease.
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- 2014
136. Persistent Exposure to
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Fengxue, Geng, Junchao, Liu, Yan, Guo, Chen, Li, Hongyang, Wang, Hongyan, Wang, Haijiao, Zhao, and Yaping, Pan
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Proteome ,Gene Expression Profiling ,tumorigenic properties ,Epithelial Cells ,long-term infection ,Microarray Analysis ,Microbiology ,Cell Line ,Cell Movement ,Humans ,inflammatory microenvironment ,OSCC ,Porphyromonas gingivalis ,Cell Proliferation ,Original Research - Abstract
Recent epidemiological studies revealed a significant association between oral squamous cell carcinoma (OSCC) and Porphyromonas gingivalis, a major pathogen of periodontal disease. As a keystone pathogen of periodontitis, P. gingivalis is known not only to damage local periodontal tissues, but also to evade the host immune system and eventually affect systemic health. However, its role in OSCC has yet to be defined. To explore the underlying effect of chronic P. gingivalis infection on OSCC and to identify relevant biomarkers as promising targets for therapy and prevention, we established a novel model by exposing human immortalized oral epithelial cells (HIOECs) to P. gingivalis at a low multiplicity of infection (MOI) for 5–23 weeks. The P. gingivalis infected HIOECs were monitored for tumor biological alteration by proliferation, wound healing, transwell invasion, and gelatin zymography assays. Microarray and proteomic analyses were performed on HIOECs infected with P. gingivalis for 15 weeks, and some selected data were validated by quantitative real-time PCR and (or) western blot on cells infected for 15 and 23 weeks. Persistent exposure to P. gingivalis caused cell morphological changes, increased proliferation ability with higher S phase fraction in the cell cycle, and promoted cell migratory and invasive properties. In combining results of bioinformatics analyses and validation assays, tumor-related genes such as NNMT, FLI1, GAS6, lncRNA CCAT1, PDCD1LG2, and CD274 may be considered as the key regulators in tumor-like transformation in response to long-time exposure of P. gingivalis. In addition, some useful clinical biomarkers and novel proteins were also presented. In conclusion, P. gingivalis could promote tumorigenic properties of HIOECs, indicating that chronic P. gingivalis infection may be considered as a potential risk factor for oral cancer. The key regulators detected from the present model might be used in monitoring the development of OSCC with chronic periodontal infection.
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- 2016
137. Functional Characterizations of Purified Ctr Copper Transporter Proteins Reveal a Novel Mechanism of Ion Selectivity and Transport
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Ming Zhou, Yaping Pan, and Kehan Chen
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Ion selectivity ,chemistry ,Mechanism (biology) ,Biophysics ,chemistry.chemical_element ,Transporter ,Copper - Published
- 2019
138. Structural basis of the alternating-access mechanism in a bile acid transporter
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Brian Kloss, Renato Bruni, Ming Zhou, Jason G. McCoy, Yaping Pan, Ruchika Sharma, Xiaoming Zhou, Matthias Quick, and Elena J. Levin
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Models, Molecular ,Rotation ,Protein Conformation ,medicine.drug_class ,Crystallography, X-Ray ,Article ,Bile Acids and Salts ,Structure-Activity Relationship ,chemistry.chemical_compound ,Bacterial Proteins ,medicine ,SLC10A2 ,SLC10A1 ,Membrane Glycoproteins ,Multidisciplinary ,Bile acid ,biology ,Cell Membrane ,Sodium ,Reproducibility of Results ,Biological Transport ,Taurocholic acid ,G protein-coupled bile acid receptor ,Yersinia ,Small intestine ,medicine.anatomical_structure ,Biochemistry ,chemistry ,Biliary tract ,biology.protein ,Carrier Proteins ,CYP8B1 - Abstract
Bile acids are synthesized from cholesterol in hepatocytes and secreted through the biliary tract into the small intestine, where they aid in absorption of lipids and fat-soluble vitamins. Through a process known as enterohepatic recirculation, more than 90% of secreted bile acids are then retrieved from the intestine and returned to the liver for resecretion. In humans, there are two Na(+)-dependent bile acid transporters involved in enterohepatic recirculation, the Na(+)-taurocholate co-transporting polypeptide (NTCP; also known as SLC10A1) expressed in hepatocytes, and the apical sodium-dependent bile acid transporter (ASBT; also known as SLC10A2) expressed on enterocytes in the terminal ileum. In recent years, ASBT has attracted much interest as a potential drug target for treatment of hypercholesterolaemia, because inhibition of ASBT reduces reabsorption of bile acids, thus increasing bile acid synthesis and consequently cholesterol consumption. However, a lack of three-dimensional structures of bile acid transporters hampers our ability to understand the molecular mechanisms of substrate selectivity and transport, and to interpret the wealth of existing functional data. The crystal structure of an ASBT homologue from Neisseria meningitidis (ASBT(NM)) in detergent was reported recently, showing the protein in an inward-open conformation bound to two Na(+) and a taurocholic acid. However, the structural changes that bring bile acid and Na(+) across the membrane are difficult to infer from a single structure. To understand the structural changes associated with the coupled transport of Na(+) and bile acids, here we solved two structures of an ASBT homologue from Yersinia frederiksenii (ASBTYf) in a lipid environment, which reveal that a large rigid-body rotation of a substrate-binding domain gives the conserved 'crossover' region, where two discontinuous helices cross each other, alternating accessibility from either side of the cell membrane. This result has implications for the location and orientation of the bile acid during transport, as well as for the translocation pathway for Na(+).
- Published
- 2013
139. Clinical and microbiologic effect of nonsurgical periodontal therapy on patients with chronic or aggressive periodontitis.
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Jingbo Liu, Jian Zhao, Chen Li, Ning Yu, Dongmei Zhang, and Yaping Pan
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CHRONIC disease treatment ,PERIODONTITIS treatment ,AGGRESSIVE periodontitis ,BACTERIA ,DENTAL plaque ,HEALTH outcome assessment ,POLYMERASE chain reaction ,RESEARCH funding ,T-test (Statistics) ,U-statistics ,TREATMENT effectiveness ,DATA analysis software ,DESCRIPTIVE statistics ,THERAPEUTICS - Abstract
Objective: To evaluate the changes in the clinical parameters, and the prevalence and quantities of three major periodontopathic bacteria, namely Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, and Tannerella forsythia, in subgingival plaque collected from patients with generalized chronic periodontitis (GCP) or generalized aggressive periodontitis (GAgP) in response to nonsurgical periodontal therapy. Method and Materials: 73 GCP patients and 57 GAgP patients were enrolled in this study. Clinical parameters, including probing depth (PD), clinical attachment loss (CAL), and Sulcus Bleeding Index (SBI) were measured. The prevalence and quantities of the three bacteria collected from the subgingival plaque were detected by real-time PCR. Both clinical and microbiologic parameters were evaluated at baseline, 4, and 12 weeks after the nonsurgical periodontal treatment. Results: PD, CAL, and SBI were significantly improved in GCP and GAgP groups at 4 and 12 weeks after nonsurgical periodontal therapy, compared to the baseline levels. The prevalence and quantities of P gingivalis in GCP at baseline (82.19% and 3.35E+5) were statistically higher than those found in GAgP (66.67% and 1.08E+5; P < .05). After therapy, the prevalence and quantities of the three bacteria were sufficiently reduced in both groups at 4 and 12 weeks. There was no significant difference in improvement of clinical and microbiologic parameters between the GCP and GAgP patients after treatment. Conclusion: There was a difference in P gingivalis prevalence and quantity between the GCP and GAgP patients at baseline. In addition, nonsurgical periodontal therapy was effective in the treatment of clinical symptoms and the major periodontopathic bacterial control between GCP and GAgP patients. [ABSTRACT FROM AUTHOR]
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- 2013
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140. Gating of the TrkH ion channel by its associated RCK protein TrkA
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Hua Huang, Ming Zhou, Yaping Pan, Elena J. Levin, Xiangshu Jin, Brian Kloss, and Yu Cao
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Models, Molecular ,Protein Folding ,animal structures ,Gating ,Biology ,Tropomyosin receptor kinase A ,Crystallography, X-Ray ,Article ,03 medical and health sciences ,Adenosine Triphosphate ,0302 clinical medicine ,Protein structure ,Bacterial Proteins ,Protein Structure, Quaternary ,Ion transporter ,Ion channel ,030304 developmental biology ,0303 health sciences ,Ion Transport ,Multidisciplinary ,Electric Conductivity ,Protein Structure, Tertiary ,Transport protein ,Adenosine Diphosphate ,Cytosol ,nervous system ,Biochemistry ,Biophysics ,Protein folding ,Vibrio parahaemolyticus ,Ion Channel Gating ,030217 neurology & neurosurgery - Abstract
TrkH belongs to a superfamily of K(+) transport proteins required for growth of bacteria in low external K(+) concentrations. The crystal structure of TrkH from Vibrio parahaemolyticus showed that TrkH resembles a K(+) channel and may have a gating mechanism substantially different from K(+) channels. TrkH assembles with TrkA, a cytosolic protein comprising two RCK (regulate the conductance of K(+)) domains, which are found in certain K(+) channels and control their gating. However, fundamental questions on whether TrkH is an ion channel and how it is regulated by TrkA remain unresolved. Here we show single-channel activity of TrkH that is upregulated by ATP via TrkA. We report two structures of the tetrameric TrkA ring, one in complex with TrkH and one in isolation, in which the ring assumes two markedly different conformations. These results suggest a mechanism for how ATP increases TrkH activity by inducing conformational changes in TrkA.
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- 2013
141. Multidisciplinary therapy for the treatment of malocclusion in a patient with chronic periodontitis with a five-year follow-up: A case report
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Hongyang Wang, Xiaoling Tang, Yaping Pan, Chen Li, Dongmei Zhang, Jingbo Liu, and Li Lin
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0301 basic medicine ,Dental practice ,Cancer Research ,medicine.medical_specialty ,Endoscope ,medicine.medical_treatment ,Orthognathic surgery ,Dentistry ,periodontal maintenance therapy ,03 medical and health sciences ,0302 clinical medicine ,Immunology and Microbiology (miscellaneous) ,Multidisciplinary approach ,medicine ,business.industry ,orthognathic surgery ,Five year follow up ,chronic periodontitis ,030206 dentistry ,General Medicine ,Articles ,medicine.disease ,Chronic periodontitis ,Surgery ,030104 developmental biology ,multidisciplinary therapy ,Malocclusion ,Risk assessment ,business - Abstract
Multidisciplinary therapy is essential in dental practice to achieve optimized outcomes. The present case report describes the application of periodontal surgery with a five-year follow-up in a patient with malocclusion and chronic periodontitis. In the presence of periodontal inflammation, orthodontic therapy may result in further periodontal breakdown due to plaque accumulation. In order to prevent this progression, scaling and root planning with a periodontal endoscope was applied, and continuous clinical monitoring and risk assessment was performed every 3 months using a Florida Probe. This combined treatment supports the long-term maintenance of periodontal conditions, functional occlusion and harmony of the facial profile.
- Published
- 2016
142. Identification of Methylosome Components as Negative Regulators of Plant Immunity Using Chemical Genetics
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Ming Zhou, Shuai Huang, Xin Li, Aruna D. Balgi, Yaping Pan, Xiao-Ran Zhang, Michel Roberge, Li-Lin Du, and Meng Li
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0301 basic medicine ,Genetics ,Hyaloperonospora arabidopsidis ,Protein-Arginine N-Methyltransferases ,biology ,Positional cloning ,Arabidopsis Proteins ,Protein arginine methyltransferase 5 ,Mutant ,Arabidopsis ,Autoimmunity ,Plant Science ,biology.organism_classification ,Plants, Genetically Modified ,03 medical and health sciences ,030104 developmental biology ,Gene Expression Regulation, Plant ,Plant Immunity ,Molecular Biology ,Chemical genetics ,Small nuclear ribonucleoprotein ,Genetic screen ,Methylosome ,Disease Resistance - Abstract
Nucleotide-binding leucine-rich repeat (NLR) proteins serve as immune receptors in both plants and animals. To identify components required for NLR-mediated immunity, we designed and carried out a chemical genetics screen to search for small molecules that can alter immune responses in Arabidopsis thaliana. From 13 600 compounds, we identified Ro 8-4304 that was able to specifically suppress the severe autoimmune phenotypes of chs3-2D (chilling sensitive 3, 2D), including the arrested growth morphology and heightened PR (Pathogenesis Related) gene expression. Further, six Ro 8-4304 insensitive mutants were uncovered from the Ro 8-4304-insensitive mutant (rim) screen using a mutagenized chs3-2D population. Positional cloning revealed that rim1 encodes an allele of AtICln (I, currents; Cl, chloride; n, nucleotide). Genetic and biochemical analysis demonstrated that AtICln is in the same protein complex with the methylosome components small nuclear ribonucleoprotein D3b (SmD3b) and protein arginine methyltransferase 5 (PRMT5), which are required for the biogenesis of small nuclear ribonucleoproteins (snRNPs) involved in mRNA splicing. Double mutant analysis revealed that SmD3b is also involved in the sensitivity to Ro 8-4304, and the prmt5-1 chs3-2D double mutant is lethal. Loss of AtICln, SmD3b, or PRMT5 function results in enhanced disease resistance against the virulent oomycete pathogen Hyaloperonospora arabidopsidis Noco2, suggesting that mRNA splicing plays a previously unknown negative role in plant immunity. The successful implementation of a high-throughput chemical genetic screen and the identification of a small-molecule compound affecting plant immunity indicate that chemical genetics is a powerful tool to study whole-organism plant defense pathways.
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- 2016
143. The mediating role of general self-efficacy in the association between perceived social support and oral health-related quality of life after initial periodontal therapy
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Liuzhong Wu, Ziming Ge, Yaping Pan, Jingwen Feng, Shuwei Zhang, and Lei Miao
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Oral health-related quality of life ,Periodontal examination ,Dentistry ,Oral Health ,Initial periodontal therapy ,03 medical and health sciences ,Social support ,0302 clinical medicine ,Quality of life ,Perceived social support ,medicine ,Humans ,030212 general & internal medicine ,Prospective Studies ,Prospective cohort study ,Periodontitis ,General Dentistry ,Periodontal Diseases ,business.industry ,Dentistry(all) ,Social Support ,030206 dentistry ,General self-efficacy ,medicine.disease ,Chronic periodontitis ,humanities ,Self Efficacy ,Clinical attachment loss ,Quality of Life ,business ,Case series ,Research Article - Abstract
Background Although initial periodontal therapy can ease some physical and psychological discomforts from periodontitis and improve patients’ oral health-related quality of life (OHRQoL), it is also vital to find positive resources from psychological and social aspects to promote the overall OHRQoL. This study aims to explore the associations of perceived social support (PSS) and general self-efficacy with OHRQoL and the mediating role of general self-efficacy in PSS-OHRQoL association after initial periodontal therapy. Methods A prospective case series study was conducted among consecutive outpatients with chronic periodontitis during the period of July 2014–April 2015. A total of 145 eligible patients responded to OHRQoL questionnaire and periodontal examination at baseline. About 4 to 5 weeks after initial periodontal therapy, 120 patients completed the second OHRQoL measurement and periodontal examination, along with PSS and general self-efficacy measurement. The Wilcoxon matched-pairs signed-rank test was used to determine the difference between baseline and post-treatment OHRQoL scores and periodontal parameters. Hierarchical linear regression analysis was used to explore the associations of PSS and general self-efficacy with post-treatment OHRQoL after adjusting for some demographic and periodontal variables. Asymptotic and resampling strategies were performed to explore the mediating role of general self-efficacy. Results Initial periodontal therapy resulted in a significant improvement in the mean total score and all domains of OHRQoL and all periodontal parameters measured. In hierarchical linear regression analysis, clinical attachment loss (CAL) was significantly and positively associated with post-treatment OHRQoL score (β = 0.265, p
- Published
- 2016
144. Effect of Danhong Injection Combined with Naoxintong Tablets on Prognosis and Inflammatory Factor Expression in Acute Coronary Syndrome Patients Undergoing Percutaneous Coronary Intervention
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Yun, Lv, Yaping, Pan, Yan, Gao, Jingqian, Lu, Yi, Li, Jie, Bai, and Jing, Zhai
- Subjects
Original Article - Abstract
Danhong is a Chinese medical component that has been broadly used to treat various cerebrovascular diseases. This work aimed to investigate the effect of Danhong injection combined with Naoxintong tablets on the short-term prognosis and expression of inflammatory factor-soluble CD40 ligand (sCD40L) in acute coronary syndrome (ACS) patients undergoing percutaneous coronary intervention (PCI).A total of 100 ACS patients with PCI were randomly divided equally into treatment and control groups. The control group was treated with conventional secondary prevention of coronary heart disease. Based on secondary prevention, Danhong injection combined with Naoxintong tablets was administered in the treatment group. The incidences of major adverse cardiovascular events and cardiac functions, including ejection fraction (EF) and six-minute walk test distance, during hospital discharge and at the third postoperative month were observed. The serum sCD40 levels at different times were also noted.There were 2 patients in the treatment group and 7 in the control group that were lost during follow-up, so the collected data were from only 48 patients in the treatment and 43 in the control group. During hospital discharge and at the third postoperative month, no significant difference in death, myocardial infarction, stroke, angina pectoris and readmission were observed between the two groups (p0.05). Upon hospital discharge, EF, six-minute walk test distance and serum sCD40L level in the two groups were not significantly different (p0.05). At the third postoperative month, EF and six-minute walk test distance in treatment group were significantly higher than those in the control group (p0.05), and the serum sCD40L level in the treatment group was significantly lower than that in the control group (p0.01). In addition, serum sCD40L levels in the two groups at the third postoperative month were significantly lower than those during hospital discharge (p0.01).Danhong injection combined with Naoxintong tablets decreased serum sCD40L level and improved cardiac functions in ACS patients undergoing PCI at 3 months following discharge, but not at discharge.Acute coronary syndrome; Danhong injection; Naoxintong tablets; sCD40.
- Published
- 2016
145. Effects of different factors influencing clinical compliance of Chinese patients with chronic periodontitis
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Wei, Si, Hongyan, Wang, Qian, Li, Xida, Zhao, and Yaping, Pan
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Adult ,Male ,China ,Treatment Outcome ,Risk Factors ,Chronic Periodontitis ,Humans ,Patient Compliance ,Female ,Prospective Studies ,Periodontal Index ,Prognosis - Abstract
The aims of this study were to determine the relationship between compliance in patients with chronic periodontitis and the efficacy of nonsurgical periodontal treatment as well as to investigate the factors that influence the compliance of patients with chronic periodontitis. These aims may be useful for determining the prognosis and improving the efficacy of treatment.Four hundred patients with chronic periodontitis were classified into three groups: the complete compliance group (S1), the erratic compliance group (S2), and the failing compliance group (S3). The patients who were in group S1 received supportive periodontal therapy (SPT) on time: they presented for their appointments within 2 months of the scheduled date; the patients who missed their appointments by more than 2 months or who completely missed a return visit one or more times, were placed in group S2; and the patients who never returned to receive SPT were assigned to group S3. They completed a questionnaire about compliance and underwent a clinical periodontal examination. SPSS 13.0 was used for the statistical analysis.Groups S2 (49.4%) and S3 (34.1%) were significantly different from group S1 (87.0%) (P.05) in their understanding that periodontal disease is always associated with other diseases. The analysis of severe pain during the treatment revealed significant differences (P.05) between group S1 (22.2%) and group S3 (39.9%). Awareness of chronic periodontitis and comfort during treatment are important factors influencing patient compliance. The distance of the patient's residence from the hospital, working hours, and attitudes of family members also affect patient compliance.Doctors should educate patients about oral health and manage their patients' comfort as much as possible during treatment, thereby enhancing patient compliance and achieving better treatment efficacy.
- Published
- 2016
146. Association between IL-1α rs17561 and IL-1β rs1143634 polymorphisms and periodontitis: a meta-analysis
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Yaping Pan, Yin Wt, and Li Lin
- Subjects
Risk ,0301 basic medicine ,Interleukin-1beta ,Disease ,Polymorphism, Single Nucleotide ,03 medical and health sciences ,0302 clinical medicine ,Gene Frequency ,Interleukin-1alpha ,Genotype ,Genetic variation ,Genetics ,Humans ,Medicine ,Genetic Predisposition to Disease ,Allele ,Periodontitis ,Molecular Biology ,Allele frequency ,Alleles ,business.industry ,Case-control study ,030206 dentistry ,General Medicine ,medicine.disease ,030104 developmental biology ,Case-Control Studies ,Meta-analysis ,Immunology ,business - Abstract
Genetic variations in human interleukin-1 (IL-1) genes are known to be involved in inflammatory disorders. The rs17561 and rs1143634 polymorphisms of IL-1α and IL-1β, respectively, have been increasingly recognized as important regulators in the development of periodontitis. However, the existence of a specific association remains controversial. Therefore, we performed a meta-analysis to explore the relationship between IL-1 polymorphism and periodontitis risk. Based on our inclusion criteria, six case-control studies were used, involving a total of 336 periodontitis cases and 366 healthy controls. Our meta-analysis results showed that the T allele of IL-1α rs17561 is positively associated with periodontitis susceptibility. In addition, carriers of this allele (TC + TT genotypes) demonstrated increased risk of this disease. The IL-1β rs1143634 T allele was also positively connected to periodontitis, with TC + TT genotype carriers being significantly more at risk. These results demonstrate that the IL-1α rs17561 and IL-1β rs1143634 polymorphisms are associated with periodontitis.
- Published
- 2016
147. Potentiation of the Kv1 Family K+ Channel by Cortisone Analogues
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Elena J. Levin, Yaping Pan, Matthias Quick, and Ming Zhou
- Subjects
Models, Molecular ,Stereochemistry ,Biochemistry ,Article ,Fluticasone propionate ,Small Molecule Libraries ,Adrenal Cortex Hormones ,medicine ,Humans ,Myocyte ,Binding site ,Fluticasone ,Binding Sites ,Chemistry ,Rational design ,Long-term potentiation ,General Medicine ,Androstadienes ,Cortisone ,HEK293 Cells ,Docking (molecular) ,Drug Design ,Shaker Superfamily of Potassium Channels ,Molecular Medicine ,medicine.drug - Abstract
The Kv1 family voltage-dependent K(+) channels are essential for termination of action potentials in neurons and myocytes. These channels form a stable complex with their beta subunits (Kvβ), some of which inhibit channel activity. Cortisone potentiates Kv1 channel by binding to Kvβ and promoting its dissociation from the channel, but its half-maximum effective concentration is ∼46 μM. To identify corticosteroids that are more efficient than cortisone, we examined 25 cortisone analogues and found that fluticasone propionate potentiates channel current with a half-maximum effective concentration (EC(50)) of 37 ± 1.1 nM. Further studies showed that fluticasone propionate potentiates channel current by inducing dissociation of Kvβ, and docking of fluticasone propionate into the cortisone binding site reveals potential interactions that enhance the EC(50) value. Thus, fluticasone propionate provides a starting point for rational design of more efficient small-molecule compounds that increase Kv1 activity and affect the integrity of the Kv1-Kvβ complex.
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- 2012
148. Structure and permeation mechanism of a mammalian urea transporter
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Ming Zhou, Giray Enkavi, Elena J. Levin, Matthias Quick, Emad Tajkhorshid, Yaping Pan, and Yu Cao
- Subjects
Models, Molecular ,Osmosis ,Urea transporter ,Molecular Conformation ,Protomer ,Molecular Dynamics Simulation ,Biology ,Crystallography, X-Ray ,Kidney ,Ligands ,Xenopus laevis ,chemistry.chemical_compound ,Animals ,Humans ,Urea ,Cloning, Molecular ,Multidisciplinary ,Urea binding ,Osmotic concentration ,Catabolism ,Membrane Transport Proteins ,Proteins ,Biological Sciences ,Metabolism ,Biochemistry ,chemistry ,Liposomes ,biology.protein ,Osmoregulation ,Cattle - Abstract
As an adaptation to infrequent access to water, terrestrial mammals produce urine that is hyperosmotic to plasma. To prevent osmotic diuresis by the large quantity of urea generated by protein catabolism, the kidney epithelia contain facilitative urea transporters (UTs) that allow rapid equilibration between the urinary space and the hyperosmotic interstitium. Here we report the first X-ray crystal structure of a mammalian UT, UT-B, at a resolution of 2.36 Å. UT-B is a homotrimer and each protomer contains a urea conduction pore with a narrow selectivity filter. Structural analyses and molecular dynamics simulations showed that the selectivity filter has two urea binding sites separated by an approximately 5.0 kcal/mol energy barrier. Functional studies showed that the rate of urea conduction in UT-B is increased by hypoosmotic stress, and that the site of osmoregulation coincides with the location of the energy barrier.
- Published
- 2012
149. Overexpression of MexAB-OprM efflux pump in carbapenem-resistant Pseudomonas aeruginosa
- Author
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Yuanhong Xu, Zhongxin Wang, Yaping Pan, Ya-ping Fang, and Jilu Shen
- Subjects
0301 basic medicine ,Imipenem ,030106 microbiology ,Microbial Sensitivity Tests ,Biology ,medicine.disease_cause ,Real-Time Polymerase Chain Reaction ,Biochemistry ,Microbiology ,beta-Lactamases ,03 medical and health sciences ,Bacterial Proteins ,Drug Resistance, Multiple, Bacterial ,Genes, Regulator ,Genetics ,medicine ,Humans ,Molecular Biology ,Gene ,Regulator gene ,Pseudomonas aeruginosa ,Membrane transport protein ,Point mutation ,Membrane Transport Proteins ,General Medicine ,Meropenem ,biochemical phenomena, metabolism, and nutrition ,Anti-Bacterial Agents ,Multiple drug resistance ,Repressor Proteins ,Phenotype ,Carbapenems ,Mutation ,biology.protein ,Thienamycins ,Efflux ,medicine.drug ,Bacterial Outer Membrane Proteins - Abstract
Efflux pump systems are one of the most important mechanisms conferring multidrug resistance in Pseudomonas aeruginosa. MexAB-OprM efflux pump is one of the largest multi-drug resistant efflux pumps with high-level expression, which is controlled by regulatory genes mexR, nalC, and nalD. This study investigated the role of efflux pump MexAB-OprM in 75 strains of carbapenem-resistant P. aeruginosa and evaluated the influence of point mutation of the regulatory genes. The minimum inhibitory concentrations of imipenem and meropenem, with or without MC207110, an efflux pump inhibitor, were determined by agar dilution method to select the positive strains for an overexpressed active efflux pump. Carba NP test and EDTA-disk synergy test were used for the detection of carbapenemase and metallo-β-lactamases, respectively. The gene mexA, responsible for the fusion protein structure, and the reference gene rpoD of the MexAB-OprM pump were amplified by real-time PCR. The quantity of relative mRNA expression was determined simultaneously. By PCR method, the efflux regulatory genes mexR, nalC, and nalD and outer membrane protein OprD2 were amplified for the strains showing overexpression of MexAB-OprM and subsequently analyzed by BLAST. Among the 75 P. aeruginosa strains, the prevalence of efflux pump-positive phenotype was 17.3 % (13/75). Carba NP test and EDTA-disk synergy test were all negative in the 13 strains. PCR assay results showed that ten strains overexpressed the MexAB-OprM efflux pump and were all positive for the regulatory genes mexR, nalC, and nalD. Sequence analysis indicated that of the ten isolates, nine had a mutation (Gly → Glu) at 71st amino acid position in NalC, and eight also had a mutation (Ser → Arg) at 209th position in NalC. Only one strain had a mutation (Thr → Ile) at the 158th amino acid position in NalD, whereas eight isolates had mutations in MexR. In conclusion, overexpression of efflux pump MexAB-OprM plays an important role in carbapenem-resistant P. aeruginosa. The mutations of regulatory genes may be a main factor contributing to overexpression of MexAB-OprM.
- Published
- 2015
150. Novel Mechanism of Gating in the TrkH-TrkA Complex
- Author
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Hanzhi Zhang, Wah Chiu, Ming Zhou, Yaping Pan, Mingqiang Rong, and Zhao Wang
- Subjects
Cytosol ,chemistry.chemical_compound ,Membrane ,chemistry ,Tetramer ,Stereochemistry ,Dimer ,Biophysics ,Protomer ,Gating ,Biology ,Ligand (biochemistry) ,Ion channel - Abstract
The superfamily of K+ transporters (SKT) is ubiquitous in bacteria, fungi and plants. SKT proteins are required for survival of bacteria in low K+ conditions and are involved in salt regulation in fungi and plants. Bacterial SKTs have two components, a membrane embedded protein that resembles an ion channel and a cytosolic protein that regulates channel gating [1]. Crystal structures of two bacterial SKT systems were reported recently [2,3]. In both structures, the membrane embedded component forms a homodimer onto which a homotetrameric ring of the cytosolic protein docks. Single-channel activities of one of the complexes, the TrkH (membrane embedded) -TrkA (cytosolic) complex, were recorded and analyzed: ATP or ATP analogs such as AMPPNP activates the channel while ADP closes it [2]. The structure of the TrkH-TrkA complex is likely in a closed conformation because it was crystallized in the presence of NADH, a ligand that does not activates the channel. In order to understand how ATP or its analogs induces channel opening, we solved the structure of the TrkH-TrkA complex in the presence of AMPPNP to 3.29 A by x-ray crystallography. When compared to the previous structures, the new structure shows that each TrkA protomer binds to two AMPPNP molecules, and that the TrkA tetramer assumes an elongated conformation that likely induces a change in the TrkH. Conformational changes in the TrkH involve significant changes in the dimer interface and are different from any other channels of known structure. These new observations and hypotheses will be validated and tested by mutational and functional analyses.[1] Levin EJ, Zhou M. Recent progress on the structure and function of the TrkH/KtrB ion channel. Curr Opin Struct Biol. 2014;27:95-101.[2] Cao Y, Pan Y, Huang H, et al. Gating of the TrkH ion channel by its associated RCK protein TrkA. Nature. 2013;496(7445):317-22.[3] Vieira-pires RS, Szollosi A, Morais-cabral JH. The structure of the KtrAB potassium transporter. Nature. 2013;496(7445):323-8.
- Published
- 2017
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