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Background: N1-Methyladenosine (m1A) is an abundant modification of transcripts regulating mRNA structure and translation efficiency. However, the characteristics and biological functions of mRNA m1A modification in adult hippocampal neurogenesis remain enigmatic. Results: We found that m1A demethylase Alkbh3 was dramatically enriched in neurons and neuronal genesis. Functionally, depletion of Alkbh3 in neural stem cells (NSCs) significantly decreased m1A modification, neuronal differentiation and proliferation coupling with increasing gliogenesis, whereas overexpressing Alkbh3 facilitated neuronal differentiation and proliferation. Mechanistically, the m1A demethylation of Mmp15 mRNA by Alkbh3 improved its RNA stability and translational efficacy, which promoted neurogenesis. Therapeutically, the silencing of Alkbh3 reduced hippocampal neurogenesis and impaired spatial memory in the adult mice. Conclusions: We reveal a novel function of m1A demethylation on Mmp15 mRNA in Alkbh3-mediated neurogenesis, which shed light on advancing Alkbh3 regulation of neurogenesis as a novel neurotherapeutic strategy. [ABSTRACT FROM AUTHOR]

Rural tourism is a new driving force for achieving rural revitalization and development, promoting rural economic prosperity, and serving as a new industrial approach to achieving the transformation and upgrading of farmers' livelihoods. This paper focuses on Manas County as the research subject and employs farmer questionnaire interviews and participatory rural appraisal methods to categorize villages into four types: "scenic area-dependent" villages, "industry-dependent" villages, "folklore-dependent" villages, and "suburban-dependent" villages. Based on the sustainable livelihood analysis framework proposed by the Department for International Development, we developed a sustainable livelihood framework for farm households in Manas County and analyzed the effects of rural tourism on farm households' livelihood capital and livelihood strategies as the watershed of the rural tourism takes shape, taking livelihood capital and livelihood strategies as the entry point. The factors influencing the livelihood capital and livelihood options of farm households in the context of rural tourism are analyzed. The results show that: (1) The overall livelihood capital of farmers engaging in rural tourism is significantly greater than that of farmers not participating in rural tourism. Additionally, variations exist in the livelihood capital of different types of rural tourism within villages. (2) Participation in rural tourism significantly influences farmers' choices of livelihood strategies. Compared with individuals who do not engage in rural tourism, those involved in rural tourism are more likely to opt for self-management livelihood strategies. The proportion of self-managed farmers in villages based around scenic areas is the highest. (3) The effect of rural tourism on farm households in Manas County is generally positive, and various types of villages yield different impact effects. (4) Participation in rural tourism significantly increases the possibility of farmers choosing self-management livelihood strategies, while it reduces the possibility of farmers choosing agriculture-oriented livelihood strategies. In all villages, except those dependent on industry, the decision to engage in rural tourism significantly impacts farmers' selection of self-management livelihood strategies. [ABSTRACT FROM AUTHOR]

Summary: In order to study the dynamic characteristics and human‐induced vibration response of light steel foamed concrete composite floor (LCSF), the vibration characteristics of 4.2 m × 5 m LCSF model were tested under the condition of opposite side support, and the natural vibration frequency of LCSF was obtained. The orthogonal anisotropic elastic plate and the simulated beam element were used, respectively, to compute the natural vibration frequency of the LCSF, and the estimated findings were compared with those obtained from measurements. The discrepancy between the calculated results and the measured results of the approximate beam element using the natural vibration frequency calculation technique is around 13%, but the error for the plate element using the calculated results and the tested results is about 23%. To examine the floor's vibration response under the factors of step frequency, walking path, pedestrian density, and load distribution, the LCSF underwent a human‐induced vibration test. The test results show that the fundamental frequency of the LCSF specimen is about 11 Hz, which can meet the requirements of the specification. However, the vibration response of the LCSF specimen under different conditions of pedestrian load is significantly different. With the acceleration of step frequency and the increase and concentration of load, the floor's vibration response becomes more visible. In the route test, it is established that LCSF has the attribute of a unidirectional plate. The natural vibration frequency of similar floor slab can be calculated by the method of simulating the natural vibration frequency of beam element. The findings can serve as a guide for LCSF research and implementation. [ABSTRACT FROM AUTHOR]