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509 results on '"Xie, Junxia"'

119. Neuroprotective effects of porphyran derivatives against 6-hydroxydopamine-induced cytotoxicity is independent on mitochondria restoration

Author

Wang, Weiwei, Song, Ning, Jia, Fengjv, Xie, Junxia, Zhang, Quanbin, and Jiang, Hong

Subjects
Original Article
Abstract

We previously reported that acetylated and phosphorylated derivatives of porphyran extracted from Porphyra haitanensis exhibit antioxidant activity in cell-free system. The aim of the present study was to investigate the neuroprotective effects of porphyran and its derivatives on 6-hydroxydopamine (6-OHDA)-induced cytotoxicity. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to measure cell viability. Changes in the mitochondrial transmembrane potential (ΔΨm) were measured by rhodamine123 using flow cytometry. The results showed that porphyran and its two derivatives, acetylated porphyran (AP) and phosphorylated porphyran (PP) (

Published
2015

120. Differential regulation of estrogen in iron metabolism in astrocytes and neurons.

Author

Xu, Manman, Tan, Xu, Li, Na, Wu, Hao, Wang, Yue, Xie, Junxia, and Wang, Jun

Subjects
IRON metabolism, BRAIN metabolism, ASTROCYTES, NEURAL physiology, DOWNREGULATION
Abstract

Previous studies have demonstrated an effect of estrogen on iron metabolism in peripheral tissues. The role of estrogen on brain iron metabolism is currently unknown. In this study, we investigated the effect and mechanism of estrogen on iron transport proteins. We demonstrated that the iron exporter ferroportin 1 (FPN1) and iron importer divalent metal transporter 1 (DMT1) were upregulated and iron content was decreased after estrogen treatment for 12 hr in primary cultured astrocytes. Hypoxia‐inducible factor‐1 alpha (HIF‐1α) was upregulated, but HIF‐2α remained unchanged after estrogen treatment for 12 hr in primary cultured astrocytes. In primary cultured neurons, DMT1 was downregulated, FPN1 was upregulated, iron content decreased, iron regulatory protein (IRP1) was downregulated, but HIF‐1α and HIF‐2α remained unchanged after estrogen treatment for 12 hr. These results suggest that the regulation of iron metabolism by estrogen in astrocytes and neurons is different. Estrogen increases FPN1 and DMT1 expression by inducing HIF‐1α in astrocytes, whereas decreased expression of IRP1 may account for the decreased DMT1 and increased FPN1 expression in neurons. In astrocytes, E2 regulates iron metabolism through upregulation of HIF‐1α, but not IRP1, to increase the levels of DMT1 and FPN1. However, in VM neurons, E2 downregulates IRP1 and decreases the levels of DMT1 and FPN1, thus playing a critical role in neuroprotection by preventing iron accumulation. [ABSTRACT FROM AUTHOR]

Published
2019
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122. Dopamine release rather than content in the caudate putamen is associated with behavioral changes in the iron rat model of parkinson’s disease

Author

Jiang Hong, Xie Junxia, Chen Wenfang, and Qian Zhong Ming

Subjects
medicine.medical_specialty, Apomorphine, Dopamine, Iron, Caudate nucleus, Deferoxamine, Iron Chelating Agents, Ferric Compounds, Dopamine agonist, Rats, Sprague-Dawley, Chlorides, Parkinsonian Disorders, Developmental Neuroscience, Internal medicine, medicine, Animals, Behavior, Animal, Chemistry, Drug Administration Routes, Putamen, Dopaminergic, Rats, Disease Models, Animal, Endocrinology, Neurology, Dopamine receptor, Catecholamine, Female, Caudate Nucleus, medicine.drug
Abstract

The effects of intranigral iron injection on dopamine (DA) release and content in the caudate putamen (CPu) and their relationship to DA-related behavioral response were investigated in rats. Different concentrations of FeCl(3) (10, 20, and 40 microg) and saline were injected separately into the left substantia nigra. In some experiments, rats were pretreated with desferrioxamine or saline before iron injection. After 3 weeks, changes in behavioral response, DA release, and DA content in the CPu were determined. In all iron injection groups (10, 20, and 40 microg), DA content in the lesioned side of the brain was significantly decreased, showing a significant linear correlation (R(2) = 0.981, P = 0.01), and DA turnover ratio significantly increased (both P = 0.01, 0.01 and 0.001 vs unlesioned sides, respectively). However, injection dosages of 10 or 20 microg of iron did not lead to significant changes in DA release in the CPu or in behavioral response. At the 40-microg dosage, it was found that DA release in the lesioned side and rearing activity both were significantly reduced (all P = 0.01 vs unlesioned side or control) and apomorphine-induced rotation was observed. Pretreatment with desferrioxamine significantly inhibited the effect of iron on DA release and content. These results demonstrate that iron injection can damage dopaminergic neurons and suggest that DA release, rather than DA content, in the CPu is associated with DA-related behavioral changes in this PD model.

Published
2003

139. Ferritin Is Secreted from Primary Cultured Astrocyte in Response to Iron Treatment via TRPML1-Mediated Exocytosis.

Author

Yu, Xiaoqi, Xiao, Zhixin, Xie, Junxia, and Xu, Huamin

Subjects
*FERRITIN, *TRIM proteins, *IRON in the body, *EXOCYTOSIS, *PARKINSON'S disease, *HOMEOSTASIS, *IRON
Abstract

Impaired iron homeostasis has been proven to be one of the critical contributors to the pathology of Parkinson's disease (PD). Ferritin is considered an intracellular protein responsible for storing cytosolic iron. Recent studies have found that ferritin can be secreted from cells independent of the classical endoplasmic reticulum–Golgi system. However, the precise mechanisms underlying the secretion of ferritin in the brain were not elucidated. In the present study, we demonstrated that the primary cultured astrocytes do have the ability to secrete ferritin, which is enhanced by iron treatment. Increased ferritin secretion was accompanied by increased protein expression of ferritin response to iron stimulation. Further study showed that iron-induced expression and secretion of ferritin could be inhibited by CQ or 3-MA pretreatment. In addition, the knockdown of transient receptor potential mucolipin 1 (TRPML1) antagonized iron-induced ferritin secretion, accompanied by further increased intracellular protein levels of ferritin. Further study demonstrated that ferritin colocalized with LAMP1 in iron-treated astrocytes. On the contrary, ras-associated protein 27a (Rab27a) knockdown further enhanced iron-induced ferritin secretion and decreased intracellular protein levels of ferritin. Furthermore, we also showed that the secretory autophagy protein tripartite motif containing 16 (TRIM16) and sec22b decreased in iron-treated astrocytes. These results suggested that astrocytes might secrete ferritin via TRPML1-mediated exocytosis. This provides new evidence for the mechanisms underlying the secretion of ferritin in primary cultured astrocytes under a high iron environment. [ABSTRACT FROM AUTHOR]

Published
2023
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145. DMT1 polymorphism and risk of Parkinson’s disease

Author

He, Qing, primary, Du, Tingting, additional, Yu, Xiaojun, additional, Xie, Anmu, additional, Song, Ning, additional, Kang, Qi, additional, Yu, Jintai, additional, Tan, Lan, additional, Xie, Junxia, additional, and Jiang, Hong, additional

Published
2011
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