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Objective To evaluate the efficacy and safety of uterine artery embolization (UAE) in the management of primary postpartum hemorrhage associated with placenta accreta. Methods We retrospectively reviewed the medical records of patients with placenta accreta between January 2010 and August 2014. Totally 18 women (mean age 30.8±4.2 years) of primary massive postpartum hemorrhage with diagnosis of placenta accrete received treatment of UAE after delivery. Images of DSA and medical records were reviewed. Technical success was defined as control of bleeding after embolization. The complications, control of hemorrhage and recurrent bleeding of the placenta left inside the uterus were retrospectively collected for assessment. Results All patients underwent transcatheter embolization of bilateral uterine arteries. The technical success rate of embolization was 100%. Bleeding was controlled in 17 of 18 patients (94%) during follow-up period (median 18 months, 3–31 months) without further bleeding recurred. One patient with placenta percreta undertook an emergent hysterectomy along with surgical bladder repair after UAE because of persistent uterine bleeding. Eight patients had postembolization syndrome and no other complications occurred. Conclusion Uterine artery embolization is an effective and safe treatment for the management of primary postpartum massive hemorrhage associated with placenta accreta.

Purpose: Pregnancy-associated breast cancer (PABC) is an aggressive disease and since China began to encourage childbearing in 2015, the incidence of PABC has increased. The study was to investigate the characteristics and survival rates of PABC patients. Methods: Patients with PABC who underwent surgery at Fudan University, Shanghai Cancer Center, from 2005 to 2018 were enrolled. First, the study divided the population into two groups: first-pregnancy group and non-first pregnancy group. Second, patients were categorized as those having breast cancer during pregnancy with/without an abortion or during the lactation period. Results: Overall, 203 patients were recruited. Since 2016, 63.9% patients were diagnosed with PABC during their second or third pregnancy, while at that time in Shanghai, only 25% of newborns were non-first births. Luminal B accounted for the highest proportion (38.4%), followed by triple-negative breast cancer (30.0%). Compared with the non-first pregnancy group, the first-pregnancy group preferred to delay treatment until the fetus was born (time from initial symptoms to treatment: 6.20 vs. 4.67 m, P=0.106). The patients in the first pregnancy group were presented with 57.0% of HR negative tumors and 26.6% of HER-2 positive tumors, while the HR negative tumors accounted for 47.6% (P=0.281) and the proportion of HER-2 positive tumors was 36.3% (P=0.108) in the non-first pregnancy group. The 3-year disease-free survival rate of the first-pregnancy group was 78.4%, and that of the non-first pregnancy group was 83.7% (P=0.325). Conclusions: Our study proves that the proportion of PABC developed during the second or third pregnancy of women was extremely large relative to the newborn populations. Patients in the first-pregnancy group tended to be presented with less aggressive tumor pathological characteristics but a worse survival outcome than those in the non-first pregnancy group.

We aim to determine the thickness of the labial plate, the distance between the cement-enamel junction (CEJ) and alveolar crest, and the inclination angle of the long axis of healthy maxillary anterior teeth by using cone- beam computed tomography (CBCT).A total of 345 CBCT volumes obtained by Newtom VGI® CBCT were analyzed by using the NNT software. The digital measurements of the labial bone plate thickness at level 4 mm below the CEJ, the midpoint of tooth root and the radiological tooth apex, the distance between the CEJ and alveolar crest, and the angle between the long axis of the teeth and the long axis of alveolar process were obtained from the mid-sagittal planes of maxillary incisors and canines. Plate thickness 4 mm below the CEJ was measured, and values below ≥1 mm were recorded.In the central incisor, 1) the angle between the long axis of the teeth and alveolar bone was 15.2°±6.2°, the distance between the CEJ and alveolar crest was (1.5±1.0) mm, labial bone plate thick-ness at 4 mm below the CEJ was (0.8±0.4) mm, the midpoint of tooth root was (0.6±0.4) mm, and the radiological tooth apex was (1.3±0.7) mm; in the lateral incisor, 16.2°±8.8°, (1.6±1.0) mm, (0.7±0.5) mm, (0.4±0.6) mm, and (1.1±0.7) mm, respectively; and in the canine, 19.0°±6.2°, (1.8±1.0) mm, (0.9±0.6) mm, (0.4±0.6) mm, and (1.2±0.7) mm, respectively. 2) The frequencies of plate thickness ≥1 mm were 28.3%, 25.8%, and 42.7% in the central incisor, lateral incisor, and canine, respectively. 3) The distance between the CEJ and alveolar crest was positively correlated with age. The correlation coefficients was 0.42 (P0.01) in the central incisor, 0.50 (P0.01) in the lateral incisor, and 0.62 (P0.01) in the canine.The thickness of labial bone plate is thin, the distance from CEJ to alveolar crest increases with age, and the long axis of the teeth is more inclined than the long axis of alveolar process. Knowledge of these special morphological characteristics can improve the safety and result for many dental procedures.目的 采用锥形束CT（CBCT）图像数字化测量的方法，测量健康上前牙唇侧骨板厚度、釉牙骨质界（CEJ）到骨嵴顶距离及牙长轴倾角，并探讨可能的影响因素。方法 分析由Newtom VGI® CBCT机拍摄获得的345例CBCT影像，使用NNT软件分别在上颌中切牙、侧切牙和尖牙的正中矢状平面上测量CEJ下4 mm、根中点和根尖水平的唇侧骨板厚度，CEJ距骨嵴顶距离和牙长轴倾角，记录各牙位CEJ下4 mm水平唇侧骨板厚度≥1 mm的例数。结果 1）中切牙的牙长轴倾角，CEJ距骨嵴顶距离，CEJ下4 mm、根中点和根尖水平的骨板厚度均值分别为15.2°±6.2°、（1.5±1.0）mm、（0.8±0.4）mm、（0.6±0.4）mm和（1.3±0.7）mm；侧切牙和尖牙的上述5项测量值分别为16.2°±8.8°、（1.6±1.0）mm、（0.7±0.5）mm、（0.4±0.6）mm和（1.1±0.7）mm；19.0°±6.2°、（1.8±1.0）mm、（0.9±0.6）mm、（0.4±0.6）mm和（1.2±0.7）mm。2）345例患者中，中切牙、侧切牙和尖牙CEJ下4 mm水平骨板厚度≥1 mm者分别占28.3%、25.8%和42.7%。3）中切牙、侧切牙和尖牙CEJ到骨嵴顶距离与年龄高度相关，相关系数分别为0.42、0.50、0.62（P0.01）。结论 健康上前牙唇侧骨板厚度较薄，CEJ到骨嵴顶距离随着年龄的增加而增加，牙长轴较牙槽突长轴舌倾，充分考虑这些特点有利于提高相关口腔治疗方案的安全性和预后。.

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This retrospective study investigated the effectiveness and safety of acupuncture as an adjunctive therapy to topical ibuprofen (TIP) for patients with chronic knee pain (CKP) due to osteoarthritis. This retrospective study analyzed medical records of 84 patients with CKP due to osteoarthritis. These patients were divided into a treatment group (n = 42) and a control group (n = 42). The patients in the treatment group were treated with acupuncture plus TIP, while the subjects in the control group received TIP monotherapy. The primary effectiveness endpoint was assessed by Western Ontario and McMaster Universities osteoarthritis index (WOMAC). The secondary effectiveness endpoints were evaluated by the numeric rating scale (NRS), 12-item Short FormHealth Survey (SF-12, mainly including mental component summary [MCS], and physical component summary [PCS]), and adverse events. All patients received an 8-week treatment. All endpoints were measured pre-treatment and posttreatment. The patients who received acupuncture plus TIP showed better effectiveness in both primary endpoint of WOMAC scale (pain, P

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In this study, we reported seven patients who underwent diagnostic evaluation through core-needle biopsy (CNB) of the spleen. After biopsy, gelatin sponge particles mixed with hemocoagulase were gradually injected using a coaxial introducer needle. One patient received microwave ablation following the CNB. All patients were followed up by computed tomography to rule out bleeding or accidental injuries both immediately after the biopsy and within 24 h. Adequate specimens for pathologic examination were obtained from all patients, and the biopsy technical success rate was 100%. No serious complications were observed in our case series. There was no evidence of postbiopsy bleeding. Therefore, injection of gelatin sponge particles mixed with hemocoagulase or microwave ablation may be effective options to prevent hemorrhage after splenic core-needle biopsies.

Lin-Wei Guo,1,2,* Lin-Miao Jiang,3,* Yue Gong,1,2,* Hong-Hua Zhang,4 Xiao-Guang Li,1 Min He,1,2 Wei-Li Sun,1 Hong Ling,1,2 Xin Hu1,2 1Key Laboratory of Breast Cancer in Shanghai, Department of Breast Surgery, Fudan University Shanghai Cancer Center, Shanghai, China; 2Department of Oncology, Shanghai Medical College, Fudan University, Shanghai, China; 3Institute for Medical Informatics, Biometry and Epidemiology, Ludwig-Maximilians Universität, Munich, Germany; 4Department of Orthopaedic Surgery, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China *These authors contributed equally to this work Purpose: TNBC is generally more aggressive than other BC subtypes and has limited therapeutic options. We aimed to construct comprehensive and reliable nomograms to predict the OS and BCSS of TNBC patients to offer clinicians therapeutic guidance for improving the prognosis of TNBC patients.Patients and methods: We used the SEER 19 Cancer Registry to identify 21,419 eligible TNBC patients diagnosed from January 1, 2010 to December 31, 2015, and divided the database randomly into a training cohort (n=10,709) and a validation cohort (n=10,710). The log-rank test and Cox analysis together with a competing risk model were utilized to identify independent prognostic factors for OS and BCSS, which were then integrated to construct nomograms.Results: According to the training cohort, except for laterality, the following factors were all predictive of OS and BCSS: age at diagnosis, race, tumor size, number of positive lymph nodes, grade, and histological subtype. The 1-, 3-, and 5-year probabilities of BC-specific mortality were 2.7%, 12.5%, and 17.1%, respectively. The precision of the nomograms was assessed by the C-index value and calibration plot diagrams. The C-index value were 0.779 for OS and 0.793 for BCSS in the internal validation and 0.774 for OS and 0.792 for BCSS in the external validation. Both internal and external calibration plot diagrams showed good consistency between the actual and predicted outcomes, especially for 3- and 5-year OS and BCSS.Conclusion: These nomograms hold promise as a novel and accurate tool in predicting OS and BCSS of TNBC patients and could be used in clinical practice to assist clinicians in developing more effective therapeutic strategies and to evaluate prognostic personally. Keywords: TNBC, nomogram, SEER, cancer-specific survival, prognosis

Intracellular tau accumulation forming neurofibrillary tangles is hallmark pathology of Alzheimer's disease (AD), but how tau accumulation induces synapse impairment is elusive. By overexpressing human full-length wildtype tau (termed hTau) to mimic tau abnormality as seen in the brain of sporadic AD patients, we found that hTau accumulation activated JAK2 to phosphorylate STAT1 (Signal Transducer and Activator of Transcription 1) at Tyr701 leading to STAT1 dimerization, nuclear translocation and its activation. STAT1 activation suppressed expression of N-methyl-D-aspartate receptors (NMDARs) through direct binding to the specific GAS element of GluN1, GluN2A and GluN2B promoters, while knockdown STAT1 by AAV-Cre in STAT1flox/floxmice or expressing dominant negative Y701F-STAT1 efficiently rescued hTau-induced suppression of NMDARs expression with amelioration of synaptic functions and memory performance. These findings indicate that hTau accumulation impairs synaptic plasticity through JAK2/STAT1-induced suppression of NMDARs expression, revealing a novel mechanism for hTau-associated synapse and memory deficits.

Intracellular tau accumulation forming neurofibrillary tangles is hallmark pathology of Alzheimer's disease (AD), but how tau accumulation induces synapse impairment is elusive. By overexpressing human full‐length wild‐type tau (termed hTau) to mimic tau abnormality as seen in the brain of sporadic AD patients, we find that hTau accumulation activates JAK2 to phosphorylate STAT1 (signal transducer and activator of transcription 1) at Tyr701 leading to STAT1 dimerization, nuclear translocation, and its activation. STAT1 activation suppresses expression of N‐methyl‐D‐aspartate receptors (NMDARs) through direct binding to the specific GAS element of GluN1, GluN2A, and GluN2B promoters, while knockdown of STAT1 by AAV‐Cre in STAT1(flox/flox) mice or expressing dominant negative Y701F‐STAT1 efficiently rescues hTau‐induced suppression of NMDAR expression with amelioration of synaptic functions and memory performance. These findings indicate that hTau accumulation impairs synaptic plasticity through JAK2/STAT1‐induced suppression of NMDAR expression, revealing a novel mechanism for hTau‐associated synapse and memory deficits.

Extracellular accumulation of amyloid-β (Aβ) forming senile plaques is one of the hallmark pathologies in Alzheimer's disease (AD), while the mechanisms underlying the neuronal toxic effect of Aβ are not fully understood. Here, we found that intracerebroventricular infusion of the aged Aβ42 in mice only induces memory deficit at 24 h but not at 7 days. Interestingly, a remarkably increased CREB (cAMP response element-binding protein) Ser133-phosphorylation (pS133-CREB) with microglial activation was detected at 24 h but not at 7 days after Aβ infusion. Aβ treatment for 24 h increased pS133-CREB level in microglia of the hippocampal non-granular cell layers with remarkably decreased pS133-CREB immunoreactivity in neurons of the hippocampal granular cell layers, including CA1, CA3, and DG subsets. Inhibition of microglia activation by minocycline or CREB phosphorylation by H89, an inhibitor of protein kinase A (PKA), abolished Aβ-induced microglia CREB hyperphosphorylation with restoration of neuronal function and attenuation of inflammatory response, i.e., reduced levels of interleukin-6 (IL6) and pCREB binding of matrix metalloproteinase-9 (MMP9) DNA. Finally, treatment of the primary hippocampal neurons with Aβ-potentiated microglia media decreased neuronal GluN1 and GluA2 levels, while simultaneous inhibition of PKA restored the levels. These novel findings reveal that intracerebroventricular infusion of Aβ only induces transient memory deficit in mice and the molecular mechanisms involve a stimulated microglial CREB phosphorylation.

Background: Cisplatin is often used for the treatment of oral cancer (OC). However, there are inconsistent results. Thus, this study plans to systematically assess the clinical efficacy and safety of cisplatin for adult patients with OC. Methods: We will search for PUBMED, EMBASE, Cochrane Library, AMED, Cumulative Index to Nursing and Allied Health Literature, Chinese Biomedical Literature Database, and China National Knowledge Infrastructure. All of them will be searched from the construction of each database up to the present with no restrictions of language and publication status. The data analysis will be conducted using RevMan 5.3 software to assess the efficacy and safety of cisplatin for adult patients with OC. Results: This study will summarize the most recent high-quality evidence and will provide helpful information about the efficacy and safety of cisplatin for adult patients with OC. Conclusion: The findings of this study will provide convinced evidence of cisplatin for adult patients with OC, and provide recommendations for clinical practice. Systematic review registration: PROSPERO CRD42019156558.

Lung cancer ranks first in incidence and mortality in China. Surgery is the primary method to cure cancer, but only 20–30% of patients are eligible for curative resection. In recent years, in addition to surgery, other local therapies have been developed for patients with numerous localized primary and metastatic pulmonary tumors, including stereotactic body radiation therapy and thermal ablative therapies through percutaneously inserted applicators. Percutaneous thermal ablation of pulmonary tumors is minimally invasive, conformal, repeatable, feasible, cheap, has a shorter recovery time, and offers reduced morbidity and mortality. Radiofrequency ablation (RFA), the most commonly used thermal ablation technique, has a reported 80–90% rate of complete ablation, with the best results obtained in tumors < 3 cm in diameter. Because the clinical efficacy of RFA of pulmonary tumors has not yet been determined, this clinical guideline describes the techniques used in the treatment of localized primary and metastatic pulmonary tumors in nonsurgical candidates, including mechanism of action, devices, indications, techniques, potential complications, clinical outcomes, post‐ablation surveillance, and use in combination with other therapies. In the future, the role of RFA in the treatment of localized pulmonary tumors should ultimately be determined by evidence from prospective randomized controlled trials comparing sublobar resection or stereotactic body radiation therapy.

There is accumulating evidence that decreased histone acetylation is involved in normal aging and neurodegenerative diseases. Recently, we found that ANP32A, a key component of INHAT (inhibitor of acetyltransferases) that suppresses histone acetylation, increased in aged and cognitively impaired C57 mice and expressing wild-type human full length tau (htau) transgenic mice. Downregulating ANP32A restored cognitive function and synaptic plasticity through upregulation of the expressions of synaptic-related proteins via increasing histone acetylation. However, there is no direct evidence that ANP32A can induce neurodegeneration and memory deficits. In the present study, we overexpressed ANP32A in the hippocampal CA3 region of C57 mice and found that ANP32A overexpression induced cognitive abilities and synaptic plasticity deficits, with decreased synaptic-related protein expression and histone acetylation. Combined with our recent studies, our findings reveal that upregulated ANP32A induced-suppressing histone acetylation may underlie the cognitive decline in neurodegenerative disease, and suppression of ANP32A may represent a promising therapeutic approach for neurodegenerative diseases including Alzheimer's disease.

The present study was aimed to investigate the electrophysiological characteristics of hippocampal postnatal early development mediated by α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors in rats. Forty-eight Wistar rats were divided into postnatal 0.5-, 1-, 2- and 3-month groups (n = 12). Spontaneous excitatory postsynaptic currents (sEPSCs) and field excitatory postsynaptic potentials (fEPSPs) mediated by AMPA receptors were recorded to evaluate the changes in the intrinsic membrane properties of hippocampal CA1 pyramidal neurons by using patch-clamp and MED64 planar microelectrode array technique respectively. The results showed that, during the period of postnatal 0.5-3 months, some of the intrinsic membrane properties of hippocampal CA1 pyramidal neurons, such as the membrane capacitance (Cm) and the resting membrane potential (RMP), showed no significant changes, while the membrane input resistance (Rin) and the time constant (τ) of the cells were decreased significantly. The amplitude, frequency and kinetics (both rise and decay times) of sEPSCs were significantly increased during the period of postnatal 0.5-1 month, but they were all decreased during the period of postnatal 1-3 months. In addition, the range of evoked fEPSPs in hippocamal CA1 region was significantly expanded, but the fEPSP amplitudes were decreased significantly during the period of postnatal 0.5-3 months. Furthermore, the evoked fEPSPs could be significantly inhibited by extracellular application of the AMPA receptor antagonist 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX). These results suggest that AMPA receptor may act as a major type of excitatory receptor to regulate synaptic transmission and connections during the early stage of hippocampal postnatal development, which promotes the development and functional maturation of hippocampus in rats.

Background Inflammation has been implicated in the development of cardiovascular disease. We determined whether nod-like receptor with pyrin domain containing 3 (NLRP3) involved in the process of prehypertension, central blockade of NLRP3 decreased inflammation reaction, regulated neurohormonal excitation, and delayed the progression of prehypertension. Methods Prehypertensive rats were induced by 8% salt diet. The rats on high-salt diet for 1 month were administered a specific NLRP3 blocker in the hypothalamic paraventricular nucleus (PVN) for 4 weeks. ELISA, western blotting, immunohistochemistry, and flow cytometry were used to measure NLRP3 cascade proteins, pro-inflammation cytokines (PICs), chemokine ligand 2 (CCL2), C-X-C chemokine receptor type 3 (CXCR3), vascular cell adhesion molecule 1 (VCAM-1), neurotransmitters, and leukocytes count detection, respectively. Results NLRP3 expression in PVN was increased significantly in prehypertensive rats, accompanied by increased number of microglia, CD4+, CD8+ T cell, and CD8+ microglia. Expressions of PICs, CCL2, CXCR3, and VCAM-1 significantly increased. The balance between 67-kDa isoform of glutamate decarboxylase (GAD67) and tyrosine hydroxylase (TH) was damaged. Plasma norepinephrine (NE) in prehypertensive rats was increased and gamma-aminobutyric acid (GABA) was reduced. NLRP3 blockade significantly decreased blood pressure, reduced PICs, CCL2, VCAM-1 expression in PVN, and restored neurotransmitters. Blood pressure and inflammatory markers were upregulated after termination of central blockage NLRP3. Conclusions Salt-induced prehypertension is partly due to the role of NLRP3 in PVN. Blockade of brain NLRP3 attenuates prehypertensive response, possibly via downregulating the cascade reaction triggered by inflammation and restoring the balance of neurotransmitters. Electronic supplementary material The online version of this article (10.1186/s12974-018-1131-7) contains supplementary material, which is available to authorized users.

To analyze the clinical features, histopathological features, treatment and prognosis of carcinosarcoma in oromaxillofacial head and neck region.The clinical and histopathological data of 10 cases with carcinosarama were collected from database of Shanghai Ninth People's Hospital and further analyzed.A total of 10 cases (8 males and 2 females) of carcinosarcoma were collected and analyzed. The age of patients ranged from 20 to 81 years (mean: 44, median:45.5). The tumor size was related to the primary site. The main clinical manifestations indicated rapid increase of foreign body sensation or mass, even feeling of pain. Pathologic examination confirmed the presence of carcinoma and sarcomatoid stroma. Carcinosarcoma had low incidence and was easily misdiagnosed. Diagnosis depended on pathologic examination especially immunohistochemistry. The prognosis was poor, and 5 year survival rate was 50%.Diagnosis to carcinosarcoma depends on pathological examination after operation. Complete resection + free flap transplantation + postoperative radiotherapy and chemotherapy are conventional treatment.

Objective To investigate the placement of a long tube into the small intestine under fluoroscopic guidance and to evaluate its decompression effect on early postoperative small bowel obstruction (EPSBO). Methods Fifty-four patients with EPSBO requiring decompression between April 2010 and July 2014 were enrolled in the study. Insertion of a long tube was guided by fluoroscopy. We first used the guide wire to pass the pylorus and then used the 10 Fr feeding tube as an exchangeable tube to put the superstiff wire into the duodenum. Finally the long tube could be passed over the guide wire through the pylorus into the intestine. The total procedure time, the radiation exposure time, and the incidence of complications were evaluated. Results The long tubes passed into the jejunum on initial insertion for all patients, so the success rate of this technique was 100%. The long tube was inserted into ileum in 18 patients. The mean total procedure time was 34.4±8.6 minutes, and the mean radiation exposure time 18.9±6.8 minutes. A total of 47 patients (87%) experienced full recovery following long-tube decompression and without the need for surgical intervention. Conclusions Using the wire-exchange technique, it is easy to place a long tube into the small bowel under fluoroscopic guidance. This decompression method is safe and effective for management of EPSBO.

Effects of Ni sheath on the microstructure, mechanical properties and bubbling phenomenon during heating process of BSCCO-2223 ceramic tapes have been investigated. Ni sheath gives Bi-2223 tapes a better ability to protect ceramic core from the damage of external force. Hardness of Ni sheath is double than that of Ag sheath reached to about 70 HV. Tensile strength of the Ni-sheathed Bi-2223 tapes is also enhanced by 80%. Harder Ni sheath is also beneficial to improve the texture of the tapes. However, serious bubbling phenomenon, which can reduce the transport property of tapes, will appear during heating process owing to the bad oxygen permeability of Ni. A lower heating rate (30°C/h) is a favorite to decrease the number and size of bubbles. In addition, Ni sheath can reduce the formation rate of Bi-2223 phase which implies that longer sintering time and higher cost of fabrication will be consumed.

Purpose To evaluate the value of CT-guided core-needle biopsy in the diagnosis of pulmonary tuberculosis. Materials and methods 67 patients suspected with pulmonary tuberculosis underwent CT-guided core-needle biopsy in our hospital between March 2009 and January 2014. The biopsy procedures, pathologic findings, and microbiologic results were recorded. Results 67 patients were enrolled including 40 men and 27 women, with a mean age of 45.4 years (range, from 17 to 75 years). Histological diagnosis were made by CT-guided core-needle biopsy as follows: The organism were detected in 20 patients; for organism negative patients, tumor tissue were found in 5 patients, epithelioid granulomatous reaction with caseation were found in 18 patients; epithelioid granulomatous reaction without caseation were found in 15 patients; nonspecific results were found in 9 patients. Smears and cultures were performed on 42 patients with TB positive in 15 patients. Finally, 51(76.12%) patients were diagnosed with TB; the specific diagnosis made by CT guided biopsy was 74.63%(50/67). Conclusion CT-guided transthoracic core needle biopsy had high performance on the diagnosis of pulmonary tuberculosis and facilitated the diagnosis of malignancy, lymphoma and other infectious disease.

Alternative splicing (AS) and its regulation play critical roles in cancer, yet the dysregulation of AS and its molecular bases in breast cancer development have not yet been elucidated. Using an in vivo CRISPR screen targeting RNA-binding proteins, we identified PHD finger protein 5A (PHF5A) as a key splicing factor involved in tumor progression. PHF5A expression was frequently upregulated in breast cancer and correlated with poor survival, and knockdown of PHF5A significantly suppressed cell proliferation, migration, and tumor formation. PHF5A was required for SF3b spliceosome stability and linked the complex to histones, and the PHF5A–SF3b complex modulated AS changes in apoptotic signaling. In addition, expression of a short truncated FAS-activated serine/threonine kinase (FASTK) protein was increased after PHF5A ablation and facilitated Fas-mediated apoptosis. This PHF5A-modulated FASTK–AS axis was widely present in breast cancer specimens, particularly those of the triple-negative subtype. Taken together, our findings reveal that PHF5A serves as an epigenetic suppressor of apoptosis and thus provides a mechanistic basis for breast cancer progression and may be a valuable therapeutic target. Significance: This study provides an epigenetic mechanistic basis for the aggressive biology of breast cancer and identifies a translatable therapeutic target. Cancer Res; 78(12); 3190–206. ©2018 AACR.

BACKGROUND Toll-like receptor 4 (TLR4) has been implicated in the progression of cardiovascular disease, including hypertension. However, the role of TLR4 in the development of prehypertension is uncertain. METHODS Prehypertensive rats were treated with 8% salt for 12 weeks to induce prehypertension. These rats were then given either TAK-242 selective TLR4 blocker, or vehicle by bilateral micro-injection to the paraventricular nucleus (PVN). Blood pressure (BP) and renal sympathetic nerve activity were recorded. PVN expression of TLR4, myeloid differentiation factor 88 (Myd88), nuclear factor-kappa B (NF-κB) p65, proinflammation cytokines (PICs), interleukin (IL)-1β, IL-6, tumor necrosis factor-alpha (TNF-α), nicotinamide adenine dinucleotide phosphate (NADPH) oxidase 2 (NOX2), NADPH oxidase 4 (NOX4), Cu/Zn superoxide dismutase (SOD) level, tyrosine hydroxylase, and 67 kDa isoform of glutamate decarboxylase (GAD67) were tested to determine the influence of TLR4 blockade. RESULTS TLR4 expression increased significantly in the PVN of high-salt groups with a corresponding increase in reactive oxygen species (ROS) and PICs. TLR4 blockade significantly reduced the signaling molecules downstream TLR4 and the expression of TNF-α, IL-6, IL-1β, decreased ROS, NOX2, NOX4 level, increased Cu/Zn-SOD, re-balanced neurotransmitters, and regulated sympathetic nerve activity in the PVN of prehypertensive rats. CONCLUSIONS Salt-induced prehypertension is partly due to the upregulation of TLR4 in PVN. Blockade of TLR4 in the brain reduced salt-induced prehypertension response, possibly through downregulation of ROS and PICs expression, and the restorage of neurotransmitter balance in the PVN.

Recent studies suggest that spatial training can maintain associative memory capacity in Tg2576 mice, but it is not known whether the beneficial effects can be inherited from the trained fathers to their offspring. Here, we exposed male wild-type and male 3XTg Alzheimer disease (AD) mice (3-m old) respectively to spatial training for one week and assessed the transgenerational effects in the F1 offspring when they were grown to 7-m old. We found that the paternal spatial training significantly enhanced progeny’s spatial cognitive performance and synaptic transmission in wild-type mice. Among several synapse- or memory-associated proteins, we observed that the expression level of synaptotagmin 1 (SYT1) was significantly increased in the hippocampus of the paternally trained-offspring. Paternal training increased histone acetylation at the promoter of SYT1 in both fathers’ and the offspring’s hippocampus, and as well as in the fathers’ sperm. Finally, paternal spatial training for one week did not improve memory and synaptic plasticity in 3XTg AD F1 offspring. Our findings suggest paternal spatial training for one week benefits the offspring’s cognitive performance in wild-type mice with the mechanisms involving an enhanced transgenerational histone acetylation at SYT1 promoter.

To evaluate the sealing ability of three different lengths of root canal fillings after post space and two different obturation techniques by using a glucose leakage test, and provide theoretical basis for clinical selection of best collocation of root canal filling methods and residual root canal filling materials.One hundred and thirty-two extracted single-root teeth were randomly divided into 6 experimental groups(n=20 each), positive(n=6)and negative controls(n=6). After root canal preparation, different treatments were performed in different groups. Group A: lateral compaction and 4 mm length of root canal fillings after post space. Group B: lateral compaction and 6 mm length of root canal fillings after post space. Group C: lateral compaction and 8 mm length of root canal fillings after post space. Group D: warm gutta vertical compaction and 4 mm length of root canal fillings after post space. Group E: warm gutta vertical compaction and 6 mm length of root canal fillings after post space. Group F: warm gutta vertical compaction and 8 mm length of root canal fillings after post space. Group G: random root canal filling. Group H: no root canal filling. A glucose leakage model was used for quantitative evaluation of the coronal to apical microleakage. SPSS 19.0 software package was used for statistical analysis.Lateral compaction showed significantly more leakage than warm gutta vertical compaction at all observation times, regardless of the length of the root canal fillings. The shorter the length of root canal fillings after post space, the worse the effect of the apical sealing for the same filling method. There was significant difference between 4 mm and 6 mm (P0.05), but no significant difference between 8 mm and 6 mm (P0.05).Warm vertical compaction shows a better sealing result than cold lateral compaction of gutta-percha. The shorter the length of root canal fillings after post space, the worse the effect of the apical sealing. 6 mm may be the proper residual root filling material length after post space preparation.

In this paper, the energy saving base on reduce voltage for motors in oil pump is analyzed, based on which the control principles of optimal slip rotating speed with maximum efficiency, minimum current, minimum input power and maximum power factor are investigated in detail, and then the PID controller that the parameter turned by BP neural network is designed，the result of experiment revealing that the essential of these control.

Aiming at the degeneration phenomenon of images taken in mist, according to features of the drop quality image, this paper proposed a novel defogging algorithm based on improved atmospheric scattering model. The problem of fog-degraded images defogging restoration is transformed into the problem of optimization estimation for original un-degraded image by maximizing the global contrast object function, in this way, the proposed algorithm can restore the object image as completely as possible in probability sense. The experimental results show that the method can effectively improve the fog degeneration phenomenon and improve image clarity, significantly improving drop quality image visual effect.

SiO2nanoparticles (NPs) are synthesized in ethanol solution and mixed with polyvinyl carbazole (PVK). The sizes of SiO2NPs are 40nm and 60nm. PVK/SiO2NPs compound systems with different sizes and with different ratios of mass fraction are obtained. Photoluminescence spectra are employed to research the optical properties of PVK molecules and PVK/SiO2NPs compound system. In compound system, the process of interface energy transfer between PVK and SiO2NPs are observed. The mainly energy transfer form is nonradiative resonance transfer.

Intelligent control is a class of control techniques that use various AI computing approaches like neural networks, Bayesian probability, fuzzy logic, machine learning, evolutionary computation and genetic algorithms. In computer science and related fields, artificial neural networks are computational models inspired by animals’ central nervous systems (in particular the brain) that are capable of machine learning and pattern recognition. They are usually presented as systems of interconnected “neurons” that can compute values from inputs by feeding information through the network. Like other machine learning methods, neural networks have been used to solve a wide variety of tasks that are hard to solve using ordinary rule-based programming, including computer vision and speech recognition.

Computer science (abbreviated CS or CompSci) is the scientific and practical approach to computation and its applications. It is the systematic study of the feasibility, structure, expression, and mechanization of the methodical processes (or algorithms) that underlie the acquisition, representation, processing, storage, communication of, and access to information, whether such information is encoded in bits and bytes in a computer memory or transcribed engines and protein structures in a human cell. A computer scientist specializes in the theory of computation and the design of computational systems. Its subfields can be divided into a variety of theoretical and practical disciplines. The computational complexity theory which explores the fundamental properties of computational and intractable problems, are highly abstract, while fields such as computer graphics emphasize real-world visual applications.

Schedules are management tools. It's important to have a schedule, but it's more important to use it. In this article it is through the proper use of project schedules that management is able to make informed decisions about scheduling activities. This use includes updating the schedules when the schedule information ceases to be useful fort making informed decisions.

In knowledge economy era, data and information become important economy resources. Drawing valuable information quickly from great amount of datum and reacting immediately, will become the key of corporation success. The paper introduces Competitive Intelligence and Corporation Competitive Intelligence System (CIS) firstly. Then, the paper gives the concept of Data Mining and general process. Furthermore, the paper gives a model of Corporation Competitive Intelligence System based on data mining and its relevant process.

Mobile agent is one of the most prominent technologies believed to be playing an important role in future e-commerce. After presented an intelligent e-commerce model based on OBI ( open buying on the internet) , we developed a modified approach for the security of mobile agents and e-commerce, and designed an intelligent shopping algorithm based on variable time negotiation function. The presented model has been evaluated by simulation experiment. It has been found that the presented model is efficient.

Recently studies have demonstrated that anthocyanins from blueberry have anticancer effects. Here, HepG2 cells were treated with anthocyanins (200、400、600、800 and 1000 μg/ml) for 48h, the effects on cell proliferation and apoptosis were investigated. The results suggested that anthocyanins can inhibit the proliferation of HepG2 cells in a dose-dependent manner. The activity of caspase-3 was increased in the anthocyanins treatment group. Moreover, results of Western blotting shown that the expression of Caspase-3 protein increased significantly in the treatment group. Taken together, our data suggest that anthocyanins could be developed as an agent against liver cancer.

Complex ability evaluation of university students is an important means of training talents to the related teaching department, and it has a very important role to improve the training quality of university and the quality of teaching. In this paper, by analyzing of the disadvantages of traditional evaluation method, an evaluation model is put forward a kind of based on fuzzy comprehensive evaluation of university student' complex ability, and give the evaluation model of the specific composition and the corresponding algorithm. The model is better than common evaluation tools to weigh the various factors in the case of making information not lossing. It can naturally deal with the initiative and fuzziness of the human mind, so it makes the every student evaluated get an objective and fair evaluation result.

Objective To evaluate the role of multidetector computed tomography-angiography (MDCTA) for management of life-threatening spontaneous retroperitoneal haemorrhage (SRH).Methods A retrospective analysis of the medical records of all SRH patients admitted to our hospital from January 1, 2005 to November 31, 2014 was performed. SRH was defined as unrelated to invasive procedures, surgery, trauma, abdominal tumor or aortic aneurysm. Life-threatening SRH was defined as hemodynamic instability with hypotension. Sixteen cases met the inclusive criteria. Patients' characteristics, clinical presentation, imaging of MDCTA, treatment modalities, morbidity, and follow-up data were analyzed.Results The group of 16 patients included 11 men and 5 women, with a mean age of 51.5±16.4 years. All patients underwent MDCTA scan examinations, and active contrast media (CM) extravasation were found in 9 patients. Angiography was performed for 8 patients in the CM extravasation group. Bleeding vessels were found in 7 patients who underwent successful embolization. One patient in the CM extravasation group did not undergo angiography and died of bleeding. Seven patients without active CM extravasation underwent conservative management. Bleeding was controlled for all these 7 patients, and 1 patient died of multiple organ failure. Fourteen patients survived from SRH were successfully discharged. One patient died of heart failure during follow-up.Conclusion MDCTA was useful in the diagnosis of SRH and proved to be predictive for the outcome of conservative treatment and early intervention.

The elastic vibrations of two-link manipulators are decoupled from the flexible manipulator for vertical planar two-links flexible manipulators. And the system was decomposed into two subsystems, slow-changing subsystem and fast-changing subsystem. An adaptive inverse controller based on model-reference is established for slow-changing subsystem. The fast-changing subsystem was deal with as disturb signal from system. The simulation results show that the proposed method not only can accurately and fast track the tip trajectory of manipulator, but also can effectively damp the elastic vibrations of two links manipulator, and is strongly robust to the variation of system parameters. The characteristic of this method is that each equivalent vibration parameters can be adaptively estimated and compensated respectively, so it is simple and easy to implement.

This retrospective study investigated the efficacy and safety of extracorporeal shock wave (EPSW) combined with hyaluronic acid (HA) for patients with knee osteoarthritis (KOA). This retrospective study included 70 patients with KOA. Of those subjects, 35 of them received EPSW combined HA, and were allocated to a treatment group, while the other 35 participants received HA alone and were allocated to a control group. Patients in both groups were treated for a total of 8 weeks. The primary outcome was measured by visual analog scale (VAS). The secondary outcomes were measured by Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC), and knee injury and osteoarthritis outcome score (KOOS). In addition, adverse events (AEs) were also evaluated. All outcomes were measured before and after the treatment. After the treatment, patients in the treatment group exhibited better efficacy in VAS (P

In order to improve the control capability of the power system voltage stability and to enhance spatial and temporal coordination of voltage control means, it is essential to establish the model of emergency voltage control that can globally mobilize reactive power support and voltage control potential. Focus on the long-term voltage stability of power system, the paper introduce nonlinear programming into emergency voltage control, settle the problem that how to establish the model of emergency voltage control. Based on detailed models of power system, the receding optimization model of long-term voltage stability control is established under framework of model predictive control. In order to improve the computational efficiency and reduce feedback delays, nonlinear programming sensitivity algorithm is proposed to solve receding optimization model. The proposed method can improve computational efficiency significantly which creates the condition for the emergency voltage control application to large-scale systems.

For the problems that the street running state and information was difficult to feedback and reasonable control, we apply the topology model to the street lighting management system, and complete the optimization control in this paper. Using the fuzzy control theory to handle the road signals, the intelligent lighting region controller integrates the thyristor chopper with the autotransformer technology, carries out the soft start and slow slope control to the street light, and realizes automatic voltage and energy saving. At finally, the system can realize the energy-saving control and intelligent, networked management of city street lamp.

It is based on actual working conditions and parameters of the conveyor belt, to reach the purpose of the optimizing rubber by different rubbers mixing technology. Comparison the five kinds rubber and metal of the friction characteristics in dynamic fit, obtained a performance excellent rubber in the specific operating conditions. Then improve the overall service life of the transportation equipment. It is first prepared the same hardness CR and EPDM and blends that is ratio of 2:8, 5:5and8:2 in this article. The pros and cons of the five rubbers are analyzed by friction and wear properties of the above experimental. The test curve of coefficient friction and wear with time has been done under different load at constant low speed, friction and wear and wear mechanism of five rubbers with chrome-plated steel comparatively analyses to determine CR/EPDM = 8:2 blends has the heat resistance and wear resistance are better than CR and EPDM under dry sliding conditions.

The research on new image segmentation algorithm is a very meaningful work in the processing of image. In the process, it will produce large amount of data redundancy. The efficient algorithm not only can greatly improve the quality of image treatment but also can greatly reduce the time and cost of the treatment. In this context, the paper analyzes several image processing algorithms commonly used in recent years and presents a new computer image processing algorithm--AMT-GA algorithm. In order to verify the effectiveness of AMT-GA algorithm, this paper takes the process of athletics image for example and compares the consistency and time of image segmentation with other literature results and ultimately finds that the consistency of AMT-GA algorithm reaches 0.99. The time in the algorithm execution is only 0.81 which not only achieves effective segmentation of the image but also saves the cost of computing. It also provides a theoretical reference for the research of computer graphics technology.

According to the problem that the intelligence is not high of the soccer robot, using the mechanical theory as a guide, making some mechanical analyses and calculations on the pressure and transmutation states of chip kick mechanics, and conducting optimal design too, then making the structure of chip kick mechanics more and more rationalization. Experiments show that the new soccer robot controller features a quick response and high servo rigidity, and provide a kind of method for improving and perfecting the soccer robot control system, at the same time, filling the needs of producing.