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The effect of changes in dissolved inorganic nitrogen (N) depositionon ecosystem functioning was investigated in the NITREX (NITRogen saturation EXperiments) project. Field-scale manipulation experiments were carried out over four to six years in seven coniferous forest ecosystems in northwestern Europe. At sites with low or moderate ambientN deposition, N was experimentally added to throughfall. At sites with high N deposition, N was removed from throughfall. We found that the capacity of the ecosystem to retain N was correlated to its internal N status. Some of the components of this N status like the N concentrations in foliage and forest floor are relatively easy to measure.The C/N ratio of the forest floor is especially closely related to the onset of nitrate leaching. Changes in N input may, in the long run, change the N status of an ecosystem due to for instance a decrease in C/N ratio in the forest floor. Decreased N input resulted in a rapid and large reduction in N concentration in drainage water. Significant improvement in tree nutritional status, tree growth, fine root biomass and diversity of ground vegetation and mycorrhizal fungi population were observed in one site only. The time period of four to six years of manipulated N deposition may have been too short for changes to be manifested in the other sites. [ABSTRACT FROM AUTHOR]

Since 1991 nitrogen deposition to a small forested catchment has been experimentally increased from ambient 11 to a total of 50 kg N ha-1 year-1 by sprinkling small portions of dissolved ammonium nitrate on about 30 occasions annually. Concentrations ofinorganic N in runoff (streamwater) were measured by flow-proportional sampling and during 6 shorter periods by frequent sampling and continuous monitoring. Shortly after the onset of the N additions, nitrate (NO3) began to appear in the runoff (streamwater), after 2 years in soil solution at shallow depths and after 4 years at alldepths. N concentrations in runoff were highly variable, and most ofthe N lost from the catchment occurred during a few episodes. Flux of N from the catchment in runoff was much larger than that calculatedfrom concentrations in lysimeter samples and discharge from groundwater levels. The annual inorganic N loss was only about 5% of the incoming N. Episodic behavior of NO3 in runoff was studied by monitoring discharge episodes after 2, 3, 4 and 6 years of N addition. The nature of NO3 concentrations in runoff during these episodes changed as the treatment proceeded. Factors such as season, hydrology, timing and size of N additions govern the loss of NO3 in runoff during episodes. Nitrogen saturation at this catchment may proceed first in the discharge areas near the outlet to uphillsoils on the slopes. [ABSTRACT FROM AUTHOR]

In June 1983 a whole-catchment liming experiment was conducted at Tjonnstrond, southernmost Norway, to test the utility of terrestrial liming as a technique to restore fish populations in remote lakes with short water-retention times. Tjonnstrond consists of 2 small ponds of3.0 and 1.5 ha in area which drain a 25-ha catchment. The area is located at about 650--700 meters above sea-level in sparse and unproductive forests of spruce, pine and birch with abundant peatlands. A dose of 3 ton/ha of powdered limestone were spread by helicopter to the terrestrial area. No limestone was added to the ponds themselves. Theponds were subsequently stocked with brown and brook trout. Liming caused large and immediate changes in surface water chemistry; pH increased from 4.5 to 7.0, Ca increased from 40 to 200 mu eq/L, ANC increased from -30 to +70 mu eq/L, and reactive-Al decreased from about 10to 3 mu mol/L. During the subsequent 11 years the chemical composition of runoff has decreased gradually back towards the acidic pre-treatment situation. The major trends in concentrations of runoff Ca, ANC, pH, Al and NO{sub}3{end} in runoff are all well simulated by the acidification model MAGIC. Neither the measured data nor the MAGIC simulations indicate significant changes in any other major ion as a result of liming. The soils at Tjonnstrond in 1992 contained significantly higher amounts of exchangeable Ca relative to those at the untreated reference catchment Storgama. In 1992 about 75% of the added Ca remains in the soil as exchangeable Ca, 15% has been lost in runoff, and10% is unaccounted for. The whole-catchment liming experiment at Tjonnstrond clearly demonstrates that this liming technique produces a long-term stable and favourable water quality for fish. Brown trout inboth ponds in 1994 have good condition, factors, which indicate thatthe fish are not stressed by marginal water quality due to re-acidification. The water quality is still adequate after 11 years and >20 water r [ABSTRACT FROM AUTHOR]

Nitrogen (N) deposition is globally considered as a major threat to ecosystem functioning with important consequences for biodiversity, carbon sequestration and N retention. Lowered N retention as manifested by elevated concentrations of inorganic N in surface waters indicates ecosystem N saturation. Nitrate (NO3) concentrations in runoff from semi-natural catchments typically show an annual cycle, with low concentrations during the summer and high concentrations during the winter. Process-oriented catchment-scale biogeochemical models provide tools for simulation and testing changes in surface water and soil chemistry in response to changes in sulphur (S) and N deposition and climate. Here we examine the ability of MAGIC to simulate the observed monthly as well as the long-term trends over 10-35 years of inorganic N concentrations in streamwaters from four monitored headwater catchments in Europe: Čertovo Lake in the Czech Republic, Afon Gwy at Plynlimon, UK, Storgama, Norway and G2 NITREX at Gårdsjön, Sweden. The balance between N inputs (mineralization+deposition) and microbial immobilization and plant uptake defined the seasonal pattern of NO3 leaching. N mineralization and N uptake were assumed to be governed by temperature, described by Q10 functions. Seasonality in NO3 concentration and fluxes were satisfactorily reproduced at three sites (R2 of predicted vs. modelled concentrations varied between 0.32 and 0.47 and for fluxes between 0.36 and 0.88). The model was less successful in reproducing the observed NO3 concentrations and fluxes at the experimental N addition site G2 NITREX (R2=0.01 and R2=0.19, respectively). In contrast to the three monitored sites, Gårdsjön is in a state of change from a N-limited to N-rich ecosystem due to 20 years of experimental N addition. At Gårdsjön the measured NO3 seasonal pattern did not follow typical annual cycle for reasons which are not well understood, and thus not simulated by the model., Capsule: The MAGIC model is able to simulate NO3 leaching on a monthly as well as an annual basis, and thus to reproduce the seasonal and short-term variations in N dynamics., (Copyright © 2015 Elsevier B.V. All rights reserved.)

We present a new formulation of the acidification model MAGIC that uses decomposer dynamics to link nitrogen (N) cycling to carbon (C) turnover in soils. The new model is evaluated by application to 15-30 years of water chemistry data at three coniferous-forested sites in the Czech Republic where deposition of sulphur (S) and N have decreased by >80% and 40%, respectively. Sulphate concentrations in waters have declined commensurately with S deposition, but nitrate concentrations have shown much larger decreases relative to N deposition. This behaviour is inconsistent with most conceptual models of N saturation, and with earlier versions of MAGIC which assume N retention to be a first-order function of N deposition and/or controlled by the soil C/N ratio. In comparison with earlier versions, the new formulation more correctly simulates observed short-term changes in nitrate leaching, as well as long-term retention of N in soils. The model suggests that, despite recent deposition reductions and recovery, progressive N saturation will lead to increased future nitrate leaching, ecosystem eutrophication and re-acidification., (Copyright © 2012 Elsevier Ltd. All rights reserved.)

Beginning in 1991, we have added nitrogen (N) to the 0.5-ha, N-poor, coniferous-forested catchment G2 NITREX at Gårdsjön, Sweden, to investigate the consequences of chronic elevated N deposition. We have added 40 kg N ha-1 yr-1 in fortnightly doses of NH4NO3 to the ambient 15 kg N ha-1 yr-1 by means of a sprinkling system. NO3 concentrations in runoff increased during 13 years from<1 to 70 microeq L-1, and in 2004 comprised about 10% of N input. Inhibition of NO3 immobilisation due to increased availability of NH4 might explain the increased leaching of NO3. C and N pools in the forest floor increased but C/N ratio has not changed. The increase in NO3 leaching thus occurred independently of change in C/N ratio. The results from Gårdsjön demonstrate that increased leaching of inorganic N and decrease in C/N ratio respond to increased N deposition at greatly different time scales.

The MAGIC model was used to evaluate the relative sensitivity of several possible climate-induced effects on the recovery of soil and surface water from acidification. A common protocol was used at 14 intensively studied sites in Europe and eastern North America. The results show that several of the factors are of only minor importance (increase in pCO(2) in soil air and runoff, for example), several are important at only a few sites (seasalts at near-coastal sites, for example) and several are important at nearly all sites (increased concentrations of organic acids in soil solution and runoff, for example). In addition changes in forest growth and decomposition of soil organic matter are important at forested sites and sites at risk of nitrogen saturation. The trials suggest that in future modelling of recovery from acidification should take into account possible concurrent climate changes and focus specially on the climate-induced changes in organic acids and nitrogen retention.

Dynamically downscaled data from two Atmosphere-Ocean General Circulation Models (AOGCMs), ECHAM4 from the Max-Planck Institute (MPI), Germany and HadAm3H from the Hadley Centre (HAD), UK, driven with two scenarios of greenhouse gas emissions (IS92a and A2, respectively) were used to make climate change projections. These projections were then used to drive four effect models linked to assess the effects on hydrology, and nitrogen (N) concentrations and fluxes, in the Bjerkreim river basin (685-km(2)) and its coastal fjord, southwestern Norway. The four effect models were the hydrological model HBV, the water quality models MAGIC, INCA-N and the NIVA FJORD model. The downscaled climate scenarios project a general temperature increase in the study region of approximately 1 degrees C by 2030-2049 (MPI IS92a) and approximately 3 degrees C by 2071-2100 (HAD A2). Both scenarios imply increased winter precipitation, whereas the projections of summer and autumn precipitation are quite different, with the MPI scenario projecting a slight increase and the HAD scenario a significant decrease. As a response to increased winter temperature, the HBV model simulates a dramatic reduction of snow accumulation in the upper parts of the catchment, which in turn lead to higher runoff during winter and lower runoff during snowmelt in the spring. With the HAD scenario, runoff in summer and early autumn is substantially reduced as a result of reduced precipitation, increased temperatures and thereby increased evapotranspiration. The water quality models, MAGIC and INCA-N project no major changes in nitrate (NO(3)(-)) concentrations and fluxes within the MPI scenario, but a significant increase in concentrations and a 40-50% increase in fluxes in the HAD scenario. As a consequence, the acidification of the river could increase, thus offsetting ongoing recovery from acidification due to reductions in acid deposition. Additionally, the increased N loading may stimulate growth of N-limited benthic algae and macrophytes along the river channels and lead to undesirable eutrophication effects in the estuarine area. Simulations made by the FJORD model and the HAD scenario indicate that primary production in the estuary might increase up to 15-20%, based on the climate-induced changes in river flow and nitrate concentrations alone.

The theme of this brief presentation is that there are tremendous advances not only in PCR-based detection but in other novel ways of detecting viruses and other agents. Much of the research is now being driven by the bio-defence agenda. Somehow those with manufacturing and regulatory responsibilities have to keep abreast of these developments and incorporate them as they become available into defining the safety of cell substrates. I am concerned that these techniques are and may remain academic research tools without validation or standardization, and that individual academic laboratories will not develop the capacity and special skills necessary to satisfy regulatory requirements, but that there is a tremendous research incentive in this area that needs to be linked with the development of safe prophylactic and therapeutic biologicals. With that, I will finish what I would consider as a report rather than an original talk.

The use of radiation shields in the head and neck cancer patient receiving adjuvant radiation therapy is a treatment alternative for protecting anticipated prosthetic implant sites. Shields can be fabricated easily as part of an interdisciplinary treatment protocol. In this article, the methods used to fabricate an extraoral radiation shield are described, and a patient treatment that illustrates possible uses of the shield for both extraoral and intraoral sites is presented.

Modifying a Super-Sep bottle by adding a brush to the lid for paint-on use allows for the precise application of the separating medium used when flasking dentures. Super-Sep offers many advantages over other commonly used separating mediums: It is virtually dimensionless, allowing for better accuracy; it dries quickly and functions best on moist stone; and it can be poured against immediately. The latter fact means less time is needed before the subsequent layers of stone can be applied when denture flasking.

The fabrication and placement of a conventional immediate denture following extraction of periodontally compromised, non-restorable remaining teeth is a method that is frequently used to help restore form and function in an esthetically pleasing manner. This technique demands knowledge in applied basic sciences and prosthodontic principles. Accurate diagnosis and scrupulous treatment planning help the dentist address the patient's needs.

Statement of Problem: Casting relief is required for proper seating of castings to allow for luting agent thickness. The application of die spacer to the die is the most common method of obtaining casting relief. Die spacer film thicknesses that are outside the ideal range of 25 to 40 microm can cause clinical problems. Thickness can be affected by the separation of die spacer constituents, which may not be reconstituted by mixing, in the bottle and by the evaporation of volatile components while the bottle is open., Purpose: The purpose of this study was to determine the effects of component evaporation and die spacer mixing technique on applied die spacer thickness., Material and Methods: Bottles of Gold Tru-fit die spacer were left open for 0, 1, 4, 8, and 24 hours at 22 degrees C. Spacer solutions were shaken either by hand per the manufacturer's directions or on a dental vibrator for 1 minute. One even brush stroke of spacer was applied to clean glass slides. Three die spacer films were made for each combination of time and mixing technique. Eighteen thickness measurements per sample at various sites were recorded with profilometer tracings. Statistical differences were determined with a 2-way ANOVA., Results: Handshaking provided greater die spacer thickness, which increased with the time that the bottle was open. Vibration provided lower thickness with no statistical increase with time., Conclusion: Insufficient agitation caused lower film thickness. Excessive evaporation caused higher film thickness.

Optimizing appropriate pharmacologic therapy in patients with congestive heart failure has been difficult due to a lack of easily obtainable hemodynamic information to guide the approach to the patient. Recently, thoracic electrical bioimpedance has allowed reliable outpatient determination of these hemodynamic variables, potentially allowing tailored adjustment of therapy in patients with cardiac dysfunction. Case studies are presented in which bioimpedance data were important contributing factors in optimizing patient management. Utilization of this new technology to individualize patient therapy should lead to improved outcomes and more efficient use of health care resources. (c)2000 by CHF, Inc.

A technique is presented for using light-cured composite resin to create desirable contours on abutment teeth for the retention and support of removable partial dentures. The desired tooth shape is created on a diagnostic cast, captured in a clear temporary splint material, and formed in resin on the abutment teeth with the splint acting as a matrix.

Retention of a facial prosthesis is the most important factor in creating a useful prosthesis for the patient. This paper presents a detailed case study of an auricular defect that was rehabilitated using two types of prosthetic retention: adhesive and osseointegration. We present the patient selection criteria, the surgical considerations prior to implant placement, retention component selection, prosthetic fabrication techniques and patient management after prosthetic delivery. The osseointegrated prosthesis made a large improvement in the patient's quality of life.

A procedure is described for the fabrication of a 3-dimensional surgical template to guide the placement of implants to retain an auricular prosthesis. This procedure requires a diagnostic wax pattern that is checked while on the patient to ensure it is positioned correctly and is also the correct size. The wax pattern is processed into a clear, methyl methacrylate resin, 3-dimensional surgical template. The most effective type of surgical template for planning implant placement is a 3-dimensional acrylic template that closely resembles the final prosthesis. This template will direct the implant placement where the retentive elements are most easily concealed, under the thickest areas of the prosthesis, which are the antihelix and antitragus. This location allows the best esthetic and functional results. An additional advantage of this technique is that it allows the retrieval of the diagnostic wax pattern of the auricle so that it can be used to fabricate the definitive prosthesis.

The combination of retentive clips and magnets has been used with favorable results for smaller facial prostheses. This patient's large midfacial defect created the need for an attachment design that would retain an intraoral obturator prosthesis and a large silicone facial prosthesis. The advantages of this design include having an obturator that is separately attached by clips to the framework. This allows the patient more ease in insertion and removal of the prosthesis. It also allows for better oral hygiene. The magnets aid the patient in the proper alignment of the facial prosthesis. The O-ring attachment was not a complicated technique in this patient's treatment. The patient can, without the use of a mirror, snap the rubber matrix O-ring attachment over the patrix attachment on the framework. This resulted in a retentive facial prosthesis, which helped the patient feel more secure. The silicone margins were well adapted to the patient's skin, which allows the use of thin translucent margins and the elimination of the inherent problems associated with the use of adhesives.

Experimental manipulations of entire ecosystems have been conducted in lakes, catchments, streams, and open terrestrial and marine environments. Experiments have addressed applied problems of ecosystem management and complex responses of communities and ecosystems to perturbations. In the course of some experiments, environmental indicators and models have been developed and tested. Surprising results with implications for ecological understanding and management are common.

To investigate the potential risk of 'nitrogen saturation' in Scandinavian boreal forests, the authors are experimentally adding 30-50 kg N ha(-1) year(-1) as NH4NO3 in precipitation to an entire 0.52-ha 80-year-old spruce forested catchment at Gårdsjön, near Gothenburg on the Swedish west coast. NO3 concentrations in runoff increased from 0 to about 7 microeq liter(-1) (maximum pulse of 43 microeq liter(-1)). The increase occurred in winter; during the April-October growing season, NO3 concentrations were very low. The speed of the response suggests that these forests are already close to saturation.

The catchments of East and West Bear Brooks, Maine, USA, have been hydrologically and chemically monitored for 3.5 years. Stream chemistries and hydrographs are similar. These clear water streams are low in ANC (0-70 microeq litre(-1)), with variations caused by changing concentrations of base cations, SO4, NO3 and Cl. The latter range between 90-120, 0-40 and 65-75 microeq litre(-1), respectively. The West Bear catchment is being treated with six applications per year of dry (NH4)2SO4 at 1800 eq ha(-1) year(-1). After one year of treatment, the response of the stream chemistry and the response modelled by MAGIC are similar. Retentions of NH4 and SO4 are nearly 100% and greater than 80%, respectively. The additional flux of SO4 is compensated principally by an increased Ca concentration. Episodes of high discharge in the treated catchment are now characterized by lower ANC and pH, and higher Al than prior to the manipulation. Concentrations of NO3 have increased about 10 microeq litre(-1) during the dormant season, presumably due to additional nitrification of N from NH4. Discharge-chemistry relationships indicate that changes in stream chemistry, except for NO3, are dominated by ion exchange reactions in the upper part of the soil profile.

Recently, the functioning of State-level expert review committees, operating under the auspices of professional medical societies, has become problematic. In particular, an increased number of State maternal mortality review committees have become inactive or disbanded primarily because of concern over liability of committee members and committee proceedings being used in litigation. A study was conducted of legal protection of the expert review process at the State level. The relevant immunity and privilege statutes of each State and the protection afforded by State law were analyzed. Findings show that, in all but a few States, the legal risk of participating in expert review is negligible. Most States have statutes that protect information involved in the review process from disclosure or use in subsequent litigation. Laws in most States also protect participants in the review process (both members of committees and providers of information) from civil liability.

The safety and efficacy of long-term oral milrinone therapy were evaluated over a 2 1/2 year period in 100 patients who had severe congestive heart failure despite conventional therapy. Long-term oral milrinone therapy (27 +/- 8 mg/day initial dose) was well tolerated; drug-related side effects occurred in only 11% of patients and led to drug withdrawal in only 4% of patients. Of 94 patients evaluated after 1 month of therapy, 51% had improved by at least one New York Heart Association functional class. Despite hemodynamic and clinical improvements, life table analysis showed a 39% mortality rate at 6 months and a 63% mortality rate at 1 year of therapy. Characteristics at study entry that predicted death within 6 months included more advanced functional class, impaired renal function, lower right ventricular ejection fraction, presence of nonsustained ventricular tachycardia on 24 hour ambulatory electrocardiography, more impaired baseline hemodynamic function and absence of clinical improvement after 1 month of milrinone therapy. Multivariate analysis selected lower baseline cardiac index and aortic systolic pressure as the most significant variables in predicting death; patients who died of progressive heart failure had less frequent use of antiarrhythmic drugs and greater increases in furosemide and milrinone doses during long-term follow-up than did those who died suddenly. Thus, although milrinone is well tolerated and produces early symptomatic benefits in approximately half of patients with congestive heart failure refractory to conventional therapy, there is no evidence that it improves the high baseline mortality in this disorder.