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In this paper volatile organic compounds (VOCs) from durum wheat cultivars and landraces were analyzed using PTR-TOF-MS. The aim was to characterize the VOC’s profile of the wholemeal flour and of the kernel to find out if any VOCs were specific to varieties and sample matrices. The VOC data is accompanied by SDS-PAGE analyses of the storage proteins (gliadins and glutenins). Statistical analyses was carried out both on the signals obtained by MS and on the protein profiles. The difference between the VOC profile of two cultivars or two preparations of the same sample - matrices, in this case kernel vs wholemeal flour - can be very subtle; the high resolution of PTR-TOF-MS - down to levels as low as pptv - made it possible to recognize these differences. The effects of grinding on the VOC profiles were analyzed using SIMPER and Tanglegram statistical methods. Our results show that it is possible describe samples using VOC profiles and protein data.

Supplementary ingredients added to basic steamed bread doughs enable the manufacture of a range of different or superior products. Some ingredients such as grains function to create variety, while others such as fiber, milk, grain, and vegetable extracts offer nutritional advantages. Processing and product quality can be improved by the addition of fats, sugar, and microingredients such as oxidants, enzymes, and emulsifiers.

The use of selected plant water extracts to control pests and weeds is gaining growing attention in organic and sustainable agriculture, but the effects that such extracts may exert on the quality aspects of durum wheat are still unexplored. In 2014, 5 plant water extracts (Artemisia arborescens, Euphorbia characias, Rhus coriaria, Thymus vulgaris, Lantana camara) were prepared and distributed on durum wheat cv Valbelice to evaluate their potential herbicidal effects. After crop harvesting, the major physicochemical and technological parameters of wholemeal flours obtained from each treatment were measured and compared with those from chemical weeding and untreated controls. A baking test was also performed to evaluate the breadmaking quality. In wholemeal flours obtained after the treatment with plant extracts protein and dry gluten content were higher than in control and chemical weeding. Wholemeal flours obtained after chemical weeding reached the highest Mixograph parameters, and that from durum wheat treated with R. coriaria extract demonstrated a very high α-amylase activity. We concluded that the treatments with plant water extracts may influence many quality traits of durum wheat. This occurrence must be taken into account in overall decisions concerning the use of plant extracts in pest and weed management practice.

BACKGROUND: Consumers expect organic produce to have higher environmental, health and sensory related qualities than conventional produce. In order to test sensory differences between bio-dynamically, bio-organically and conventionally grown winter wheat (Triticum aestivum L., cv. Runal), we performed double-blinded triangle tests with two panels on dry wholemeal flour from the harvest years 2006, 2007 and 2009 and from two field replicates of the ‘DOK’ long-term farming system comparison field trial near Basel, Switzerland. Yield and quality parameters were also assessed. RESULTS: Significant farming system effects were found for yield (up to 42% reduction in the organic system), thousand kernel weight, hectolitre weight and crude protein content across the three years. In the triangle tests one out of 12 pair-wise farming system comparisons (PFSCs) on wholemeal flour made from the different wheat samples showed significant sensory differentiation (between bio-dynamically and conventionally grown wheat). When all data from the three harvest years and two panels were aggregated, a statistically significant effect (P = 0.045) of PFSCs on the number of correct answers became evident. CONCLUSIONS: Although testing of dry wholemeal flour was very challenging for panellists, we were able to show that sensory differences between farming systems can occur. Copyright © 2012 Society of Chemical Industry

The determination of flour end-use quality of progenies is a routine process in high-quality wheat breeding program. Traditional determination involves larger amount of flour and specific instruments, meanwhile, is expensive and time-consuming. A rapid and accurate method which could predict the quality of early generation wheat lines is in high demand in the wheat breeding program. Clarifying the relationships among different prediction indexes is the prerequisite for devising a new prediction method screening the high-quality progenies. This study aimed to clarify the correlations between SDS sedimentation value, SIG and SRCs for both wholemeal flour and flour, as well as to study which parameter could be applied to predict the flour quality of spring wheat rapidly and conveniently. The result of the study showed that WSRC correlated significantly with all the parameters determined, the four different SRCs varied significantly between hard wheat and soft wheat, while SDS sedimentation value and SIG showed no significant difference between hard wheat and soft wheat. SRC test may provide a convenient method for predicting the quality of early generation wheat lines in wheat breeding.

Production of whole-grain products can provide more variety in bakery products and might impact positive health effects due to fermentable soluble fibers. To assess technological quality, micro-scale methods using 10 g of white flour are usually carried out, in particular if amount of material or additives is limited. Using wholemeal flour for micro-scale testing might enhance use efficiency of raw material. Up to date, there is no information, whether micro-scale testing of wholemeal flour provides the same information on techno-functionality as classical methods. Two wheat varieties with the same protein concentration but different bread-making performance were used to analyze this aspect. Micro-scale baking test was optimized for wholemeal flour concerning dough consistency and fermentation time. Wholemeal flour reduced loaf volume, but optimization was successful. Although all processing steps of wholemeal baking test were adapted, differences in loaf volume between the two varieties were only present with white flour. Coarse particles in wholemeal flour may be responsible for reduced gas retention. Decreasing particle size of bran material might reduce adverse effects on loaf volume. A micro-scale baking test using wholemeal flour would have several advantages after correct implementation: lower price, less time and material required, direct measurement of quality aspects.

To evaluate the effect of genotype, year and location on 1000 kernel weight, falling number, alpha- and beta-amylase activity, pasting properties, β-glucan, fructan, total starch, amylose, resistant starch and dietary fibre contents of wholemeal flour, four einkorns and one control bread wheat were cropped in Italy for two years in four different locations. Year and genotype exerted major effects on most traits, while location was not important. All einkorns showed greater fructan (on average, 1.90 vs 1.29 g/100 g dm, respectively) and lower β-glucan (0.48 vs 0.84), total starch (59.6 vs 67.9), resistant starch (2.56 vs 5.52) and dietary fibre (16.7 vs 21.5) content than the bread wheat control. The significant variation for fructan content among the four Triticum monococcum genotypes assessed indicates the possibility of breeding einkorn lines with high prebiotic activity.

BACKGROUND: To verify the viscous potential of rye bread made from population and hybrid cultivars, the overall extract viscosities (EVs) of endosperm and wholemeal breads (EBs and WMBs respectively) were assessed using extractants of different pH. Also, arabinoxylan-dependent EV (AX-EV) was determined after combined action of starch- and protein-degrading enzymes. RESULTS: The synergistic effect of a combination of α-amylase, amyloglucosidase and protease on EV was almost two times higher than the effects observed after single addition of each of them. However, water-extractable arabinoxylans (WE AXs) were the major contributors to EV. Unexpectedly, a decrease in the mean WE AX contents observed in the following order, WMB (31.7 g kg−1) and EB (29.3 g kg−1) of hybrid rye and WMB (28.8 g kg−1) and EB (23.8 g kg−1) of population rye, was accompanied by an increase in their AX-EVs (12, 21, 21 and 29 mPa s respectively). CONCLUSION: In some cases the structure of WE AXs is a more important determinant of AX-EV than their content in rye bread. Nevertheless, both factors influence AX-EV, which may reflect the viscosity of the human small intestine after rye bread consumption, related to some beneficial metabolic effects. Therefore AX-EV can be a preliminary parameter for selection towards high-viscosity bread. Copyright © 2010 Society of Chemical Industry

The trienzyme digestion including protease, α-amylase, and conjugase (γ-glutamyl hydrolase) for the extraction of total folate from cereal grain products used in AOAC Official Method 2004.05 was optimized using response surface methodology. Digestion times of α-amylase (p0.05) and conjugase (p0.01) showed significant effects on the response (total folate). Digestion time for Pronase digestion was not significant (p0.1). Ridge analysis showed that total times required for the trienzyme digestions ranged from 6.1 h for buckwheat to 8.7 h for CRM 121 (wholemeal flour). The predicted maximal value for CRM 121 (51 μg/100 g) was close to the certified value (50 ± 7 μg/100 g). The optimized extractions for the four cereals suggest that a generalized trienzyme extraction for cereals of 1, 2.5, and 6 h for Pronase, α-amylase, and conjugase, respectively, could be used to approximate total folate in cereals and cereal products. These conditions provide data comparable to AOAC Official Method 2004.05 for the cereals included in this study and would be suitable to approximate total folate in most cereals and cereal products. Compared to the official method, the shortened digestion provides cost savings and minimizes the folate loss possible with prolonged digestions.

Glycemic responses to most of the conventional breads are high, including breads made of wholemeal flour. Baking technology is known to affect these responses. The aim of the present study was to investigate effects of xylanase enzyme treatment and sourdough fermentation in wholemeal wheat bread baking on postprandial glucose and insulin responses and on in vitro protein digestibility. The wheat breads were made of 100% flour from peeled kernels by a straight dough or sourdough fermentation method, and with or without using xylanase during mixing of dough. Standard white wheat bread was used as a reference. All test bread portions contained 50 g available carbohydrate and were served in random order to eleven insulin resistant subjects. Blood samples for measuring glucose and insulin concentrations were drawn over 4 h. The sourdough wholemeal wheat bread resulted in the lowest postprandial glucose and insulin responses among the four tested breads (treatment × time; p = 0.000 and p = 0.022, respectively). There were differences in solubility and depolymerisation of protein and arabinoxylan among the breads but these did not fully explain the in vivo findings. In conclusion, the health effects of wholemeal wheat bread can be further improved by using sourdough process in breadmaking.

The kinetics of tocols degradation in wholemeal and white flours from einkorn cv Monlis and bread wheat cv Serio were studied during storage at five different temperatures (−20, 5, 20, 30 and 38 °C) up to 242 days. Tocols decreased as a function of time and temperature, following first-order kinetics. Degradation rates and their dependence upon temperature were similar for the two Triticum species studied. Tocols decrease was quicker in white flour than in wholemeal flour (on average for total tocols at 38 °C, k = 7.79 × 10 −3 days −1 vs 3.15 × 10 −3 days −1 , respectively). The temperature-dependent degradation was similar for all homologues ( Ea = 34.3–49.4 kJ/mol) except for α-T, less thermostable in white flours ( Ea = 61.2 kJ/mol in einkorn and Ea = 54.7 kJ/mol in bread wheat). The results clearly indicate that storing flours under refrigeration or at temperatures not exceeding 20 °C better preserved their tocols contents.

Bread, cakes and biscuits were manufactured from flour containing deoxynivalenol (DON) and low concentrations of zearalenone (ZON) and nivalenol (NIV). The results show that these mycotoxins remain mostly unaffected during manufacture. Although the results indicate that the mycotoxins are largely stable and survive processing, when concentrations were determined on an ‘as is’ basis as stipulated in legislation, levels in finished products were usually lower than in the starting flour due to the dilution effect of other ingredients such as fat, sugar and water. Thus mean concentrations of DON in bread were reduced by about 35% and 39% in white and wholemeal bread respectively which are in close agreement with the reduction required by the regulations although the changes that occur during milling white and wholemeal flour from whole wheat also need to be taken into account. The reduction of DON during cake manufacture is greater than for bread because flour makes up only about 25% of the starting ingredien...

Einkorn ( Triticum monococcum ssp. monococcum ) is an underutilised wheat with high protein, lutein and tocols content, particularly suited for baby and specialty foods. To study the influence of kernel steaming treatments on chemical and technological properties, seeds of five einkorn accessions and one bread wheat control (cv. Blasco) underwent different steaming conditions, and their wholemeal flours were compared for ash, protein, lutein, tocols and α-amylase content, SDS sedimentation volume and gelatinisation parameters. Furosine, a heat damage marker, was measured as well. The treatments significantly influenced most of the parameters. In particular, lutein, tocols and α-amylase diminished after steaming; SDS sedimentation volumes and most gelatinisation parameters also decreased, whereas gelatinisation temperatures and furosine contents increased. The changes were stronger under more drastic steaming conditions, although treatment × genotype influence was sometimes detected. Steaming induced migration of lutein and tocopherols from the bran and germ fractions to the kernel endosperm.

The quality of three gluten free spaghetti typologies, made from amaranthus flour plus the addition of quinoa (CQA), broad bean (CBA) or chick pea (CCA), was investigated. The quality of the produced spaghetti was compared to that of spaghetti made of durum semolina (CTRL). Tests were run on the samples to determine breakage susceptibility of dry spaghetti, the cooking resistance, instrumental stickiness in cooking and over cooking, cooking loss and some sensorial attributes at the optimal cooking time. The spaghetti obtained from amaranthus flour when compared to the CTRL, had equal or lower dry breakage susceptibility and cooking resistance, higher cooking loss and visibly lower instrumental stickiness. However, these spaghetti did not demonstrate relevant sensorial differences.

The effect of packaging materials on the physicochemical and rheological characteristics of iron-fortified wholemeal flour (WMF) during storage was determined. WMF was fortified with three fortificants, namely ferrous sulfate (30 ppm), ferrous sulfate + ethylenediamine tetraacetic acid (EDTA) (20 + 20 ppm) and elemental iron (60 ppm). Each flour was also fortified with 1.5 ppm folic acid. Moisture, flour acidity and peroxide value increased during storage, while protein and fat contents decreased. Highest conversion of Fe2+ into Fe3+was observed in flour fortified with ferrous sulfate (2.72%), followed by that fortified with ferrous sulfate + EDTA (1.49%) and elemental iron (1.06%). Water absorption and dough viscosity of iron-fortified flours increased during storage. The flour containing ferrous sulfate was most acceptable regarding sensory characteristics, followed by samples containing ferrous sulfate + EDTA. Fortified flours were more stable during storage than unfortified. Addition of EDTA increased the stability of flours and fortificants. The fortified flours stored in polypropylene bags proved more stable than those stored in the tin boxes. PRACTICAL APPLICATIONS The main role of packaging is to protect the product during handling, distribution and storage against environmental and mechanical hazards. The success of a fortification program depends on the stability of micronutrients and food to which these are added. Chemical changes during storage badly affect chapatti making and sensory properties. Exposure of the fortificant to any factor including heat, moisture, air or light, and acid or alkaline environments during processing, packaging, distribution, or storage affects its stability. Flour containing elemental iron and ferrous sulfate with EDTA remained stable up to 42 days. The unfortified flour and flour containing ferrous sulfate remained stable for 21 days in tin boxes and 28 days in the polypropylene bags. Wheat flour milling industry would be benefited from this research if government is keen to launch iron fortification program in the country to curb iron deficiency anemia among population.

Summary Three formulations of tarhana, a traditional Turkish fermented yogurt-wheat mixture used in soups, were prepared in the laboratory. The effects of white or wholemeal wheat flours and amount of yogurt on the protein breakdown, rheology and acceptability of the samples were measured and the samples were compared with single samples of homemade and commercial tarhana from Turkey. Replacement of white wheat flour with wholemeal flour and increasing the yogurt had little effect on protein breakdown, but the true- to crude-protein ratio fell slightly during fermentation. Tarhana soups behaved as pseudoplastics (n 0.8) closer to Newtonian flow (n= 1) were most liked in mouth feel; however high apparent viscosity and samples with less liked mouth feel were not less liked in overall acceptability than the commercial sample. In vitro digestibility of commercial tarhana was lower than the laboratory prepared tarhana.

The aim was to study wheat flour parameters relevant to suitability for production of chapattis, made from British wheat cultivars. Wholemeal flours from eight British cultivars grown in UK differed in physicochemical and rheological properties, and were less suited to chapatti production than Mehrani, commercial wholemeal chapatti flour. Chapatti quality of Mehrani wholemeal flour was rated as excellent, hence this was used as a benchmark. Flours from cultivars Riband and Galahad gave weak dough whereas those from Fresco and Mercia yielded dough high in resistance to sheeting that contracted after rolling. Dough from Avalon, Hereward and Pastiche had moderate resistance to sheeting properties. Changes in softness of chapatti during storage were evaluated using an Instron testing machine. Evaluation by sensory panel indicated that all cultivars produced chapattis that were fair to good in sensory characteristics. Some correlations were found between sensory and instrumental attributes of chapatti.

The physical structure of the wheat berry is described. The evaluation of wheat quality as it arrives at the flour mill is detailed. The cleaning and preparation of wheat deliveries before the flour milling process are described. The main stages of the flour milling process and the functions of the break, reduction, purifier and sieving operations are detailed for the manufacture of white flour. An example of the individual machine flours which comprise a typical straight run white flour is given. Additions which may be made at the flour mill are considered. Setting flour specifications and evaluating flour properties of end-use suitability are reviewed. Food safety considerations in the milling process are considered.

Rye flours with extraction rate of 100% (wholemeal flour), 95% (brown flour), 90% (brown flour) and 70% (light flour) were prepared in order to study the relation between flour extraction rates and content of bioactive compounds. The following compounds were analysed: total phenolic compounds (TPC), total flavonoids (TF), inositol hexaphosphate (IP6), reduced (GSH) and oxidized (GSSG) glutathione, tocopherols (T) and tocotrienols (T3). The reduced/oxidized glutathione status (GSH/GSSG) of the flours was examined as a potential index of flour resistance against oxidative stress. The following observations were made in relation to the flour extraction rates and bioactive compounds contents: (a) milling process caused decrease in content in TPC, TF, IP6, GSH and GSSG, T and T3, (b) the most resistant against oxidation processes were suggested a brown flours, then light and finally wholemeal flour, (c) the ratio of tocotrienols to tocopherols (T3/T) was the highest in rye flours with extraction rate of 100–90% whereas light flour was the poorest source of tocopherols and tocotrienols. The provided data support current trend to increase number of rye products from wholemeal or brown flours.

Mixtures obtained by the addition to semolina of wholemeal flour derived from durum wheat kernels harvested at the milky phase were analysed for their levels of fructans, vitamin C, glutathione and for their rheological characteristics to evaluate the possibility of obtaining wholemeal with increased nutritional value for preparing functional foods, without addition of exogenous additives or antioxidants. Wholemeal from immature kernels (WIK) contained almost three times more fructans than semolina from mature kernels (SMK). Moreover, wholemeal from immature kernels contained much higher amounts of ascorbate and glutathione than SMK. Consistently, wholemeal from immature kernels also had higher total antioxidant capability than SMK. The addition of a certain percentage of WIK to SMK conferred an increased tenacity to the dough. The role of the different biochemical properties, mainly related to the ascorbate redox enzymes, of WIK versus SMK in determining the rheological properties of the mixtures is discussed.

The efforts for promoting the consumption of food of plant origin are increasingly growing. The amaranth grain is an important vegetable protein source, superior in content and quality to traditional cereals. In the central-west region of Argentina, new amaranth varieties have been obtained to optimize its agronomic traits and promote its use. In this work, the analysis of the wholemeal flour protein from seeds of two new varieties of Amaranthus cruentus var. Candil (CC) and Amaranthus hypochondriacus var. Dorado (HD), as well as from advanced lines of Amaranthus hypochondriacus x Amaranthus cruentus H17a (H17) and Amaranthus cruentus G6/17a (CG6), was carried out in order to elucidate their nutritional contribution to human diet. The amino acids profile and the chemical score (CS) were determined, and the protein quality was evaluated in-vivo through the following indexes: net protein utilization (NPU), true digestibility (tD), biological value (BV) and protein digestibility corrected amino acid score (PDCAAS). In general, the amino acids values of the different varieties exceeded the requirements established by the WHO/FAO/UNU; however, valine was the limiting amino acid in all cases. The values obtained (%) were within the following ranges: NPU, 33.56–46.04 %; tD, 68.80–75.40 %; BV, 44.53–64.28%; and PDCAAS, 23.69–36.19 %. These results suggest that the new amaranth flours varieties can be adequate for human consumption and as complementary protein source. Fil: Aguilar, Elba Graciela. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina Fil: Albarracín, Graciela de Jesús. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina Fil: Uñates, María Angelina. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina Fil: Piola, Hugo Daniel. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina Fil: Camiña, José Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Ciencias de la Tierra y Ambientales de La Pampa. Universidad Nacional de La Pampa. Facultad de Ciencias Exactas y Naturales. Instituto de Ciencias de la Tierra y Ambientales de La Pampa; Argentina Fil: Escudero, Nora Lilian. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia; Argentina

The impact of microbial transglutaminase (TGM) on the sensory, mechanical and crumb grain pattern of fresh pan breads has been investigated in white and wholemeal flour sponge dough samples supplemented with amylolytic-(NMYL) and non-amylolytic (PTP) enzymes. Assessment of bread performance has been carried out by physico-chemical measurements, texture profile analysis, sensory evaluation, digital image analysis and multivariate data handling. Improving effects of TGM addition were prominent when added to low extraction rate flours (increased cohesiveness by 11%, volume, aroma intensity by 31%, typical taste and crumb to cell ratio by 25% and decreased cell number by 17%). For white and whole flours, NMYL addition to TGM breads led to 16% softer breads. For the pool of enzyme supplemented samples tested, it was observed that the higher the specific volume of breads, the lower the acidification power, the better the mechanical behaviour with high cohesiveness and low hardness values and the higher the sensory scores for visual and textural attributes.

Analysis of total folate concentration measured by microbiological assay in a variety of foods submitted in a routine manner to experienced laboratories that regularly perform folate analysis on fee-for-service basis was evaluated. Homogenates of fresh strawberries, frozen spinach, orange juice, frozen meat and vegetable pizza, dry macaroni, and dried pinto beans were prepared and stored under conditions previously determined to maintain stability of folate content. An aliquot of each composite and of 3 certified reference materials were sent on each of 4 occasions to 4 laboratories. Results for macaroni and pizza, the only folic acid-fortified foods, had considerably lower between-laboratory variation (CVB) with CVB of 9–11% versus >45% for other foods. Mean total folate ranged from 14 to 279 μg/100 g for a mixed vegetable reference material, from 5 to 70 μg/100 g for strawberries, and from 28 to 81 μg/100 g for wholemeal flour. Only 1 laboratory reported using a tri-enzyme extraction, and all laboratories used folic acid fortified foods as internal control materials. Users of commercial total folate analysis should understand the uncertainty in values determined by microbiological assay, particularly for foods containing primarily naturally occurring folate, which may not be apparent when replicate samples are not submitted for analysis.

The proximate composition, nutritionally valuable minerals and the effect of some salts (NaCl, NaNO , Na SO , Na CO , CH COONa) on the food properties of Triticum durum wholemeal flour were 3 2 3 2 3 3 investigated on dry weight basis. Both the protein and carbohydrate values were high with respective values of 20.21g/100g and 65.7g/100g. Many minerals were either not detected or low but phosphorus was high with a value of 4285.71mg/100g. The lowest gelation concentration varied between 2.0 in Na CO (0.5% w/v) 2 3 and 10.0 in CH COONa and Na SO (15.0% w/v). The highest value of water absorption capacity was 3 2 3 230.33g/100g at 2.0% w/v CH COONa. The oil emulsion capacity varied from 20.0g/100g at 1.0% w/v Na CO 3 2 3 to 80.0g/100g at 0.5%w/v NaCl. The oil emulsion stability (cm ) was generally good in most of the salts and 3

Marine-based food supplements can improve human nutrition. In an effort to modulate glycaemic response and enhance nutritional aspects, marine-derived algal food rich in astaxanthin was used in the formulation of a model food (wholemeal cookie). Astaxanthin substitution of cookies made from three flours (wheat, barley and oat) demonstrated a significant reduction in the rate of glucose released during in vitro digestion together with an increase in the total phenolic content (TPC) and antioxidant capacity of the food. The significantly (p < 0.005) lower free glucose release was observed from cookies with 15% astaxanthin, followed by 10% and then 5% astaxanthin in comparison with control cookies of each flour. Total phenolic content, DPPH radical scavenging and Oxygen Radical Absorbance Capacity (ORAC) value also notably increased with increase in astaxanthin content. The results evidence the potential use of microalgae to enhance the bioactive compounds and lower the glycaemic response of wholemeal flour cookie.

Wheat is primarily used for bread-making. However, fungal diseases, grain moisture at harvest and low-protein contents strongly influence the quality of the wheat flour, thus creating challenges for traders, millers and commercial bakers who struggle to produce consistently high-quality products. This paper address the replacement of low-protein/wholemeal flour functionality for bread-making purposes. Three hydrocolloids, xanthan gum, dextran and hydroxypropyl methylcellulose, were incorporated into bread recipes based on high-protein flours, low-protein flours and coarse wholemeal flour. Hydrocolloid levels of 0–5 % (flour basis) were used in bread recipes to test the water absorption. The quality parameters of dough (farinograph, extensograph, rheofermentometre) and bread (specific volume, crumb structure and staling profile) were determined. Results showed that xanthan had negative impact on the dough and bread quality characteristics. HPMC and dextran generally improved dough and bread quality and showed dosage dependence. Volume of low-protein flour breads were significantly improved by incorporation of 0.5 % of the latter two hydrocolloids. However, dextran outperformed HPMC regarding initial bread hardness and staling shelf life regardless the flour applied in the formulation.

hemical composition plant parts (roots, stalks, leaves, blossoms) of common buckwheat (Fagopyrum esculentum Moench) and selected products made from its seeds (peels, whole seed, wholemeal flour, broken seeds, crunchy products Natural and Cocoa, flour, and pasta) was determined. Samples were dried and ground to a fine powder. All analyses were performed according to the Commission Regulation no. 152/2009, while rutin concentration was determined by the modified HPLC method. The lowest content of moisture was found in roots (4.3%) and in peels (almost 8%) and the highest moisture (nearly 11%) was discovered in seeds. The lowest amount of crude protein (3.5%) was found in peels, the highest crude protein amount (>13%) in both flours and leaves (23%). The starch content (>50% in dry matter) differs from one sample to another. Only in peels the content of starch was about 3.5%. From all examined samples, the lowest content of fat was found in crunchy products Cocoa, 1.7%. The lowest amount of histidine was determined in all studied samples, except peels, the highest content of glutamic acid was determined in almost all samples, except peels. Whole-meal flour is very rich source of Ca and Fe. The content of these elements was 1172 mg.kg-1 and 45.9 mg.kg-1, respectively. On the other hand, the highest content of Pb (>1 mg.kg-1) was found in broken seeds. The greatest concentration of rutin was determined in blossoms and leaves (83.6 and 69.9 mg.g-1), respectively. On the other hand, the lowest concentrations of rutin were found in buckwheat products (generally less then 1 mg.g-1, i.e. in wholemeal flour, 702 μg.kg-1, the lowest (almost 10 μg.kg-1) in pasta.

In 1989, the Community Bureau of Reference started a research program to improve the quality of vitamin analysis in food. To achieve this task, vitamin methodology was evaluated and tested by interlaboratory studies and the preparation of certified reference materials, which will be used for quality control of vitamin measurements. The main improvements in methodology were achieved by testing and standardizing the extraction condition and enzymatic hydrolysis procedures. Results for each individual material are derived from five replicate determinations using at least two independent methods: Liquid chromatography (HPLC) and microbiological assay for vitamins B1, B2, and B6; and radioprotein binding and microbiological assays for vitamin B12. The certificate of analysis for four reference materials gives mass fraction values for water-soluble vitamins. These certified values were based on the acceptable statistical agreement of results from collaborating laboratories. Certified values with uncertainties (mg/kg dry matter) for each CRM are as follows: 4.63 (0.20) and 4.10 (0.51) for vitamins B1 and B6, respectively, in CRM 121 (wholemeal flour); 6.51 (0.24), 14.54 (0.3), 6.66 (0.43), and 0.034 (0.003) for vitamins B1, B2, B6, and B12, respectively, in CRM 421 (milk powder); 3.07 (0.17) and 4.80 (0.40) for vitamins B1 and B6, respectively, in CRM 485 (lyophilized mixed vegetables), and 8.58 (0.55), 106.8 (2.8), 19.3 (1.5), and 1.12 (0.044) for vitamins B1, B2, B6, and B12, respectively, in CRM 487 (lyophilized pig liver).

The present research assessed the radical scavenging activity against the synthetic 2,2-diphenyl-1-(2,4,6-trinitrophenyl)hydrazyl radical of the water-saturated 1-butanol extracts of wholemeal flour from 63 Triticum monococcum accessions, which originated from different eco-geographical areas. On average, T. monococcum had higher radical scavenging activity than T. turgidum and T. aestivum species, which served as controls (0.90, 0.64 and 0.70 mmol TE/kg DM, for T. monococcum, T. turgidum and T. aestivum , respectively). A significant correlation between radical scavenging activity and tocol content was observed, while no correlation existed with soluble phenolics and carotenoid contents.

The slurry technique was applied to the determination of Ni, Cr and Co in wheat flour by electrothermal atomic absorption spectrometry (ETAAS). The influence of the graphite furnace temperature programme was optimized. Optimum sensitivity was obtained by using a mixture of 15% HNO(3)-10% H(2)O(2) as suspended medium for a 3% w/v slurry in the determination of Ni; lower concentrations of HNO(3) were necessary for the determination of Co and Cr (viz. 5 and 10%). The precision of direct analyses of the slurries was improved by using mechanical agitation between measurements; thus, the RSD of the measurements was ca. 5% for repeatability. The direct slurry sampling (SS) technique is suitable for the determination of Ni and Cr in wheat flour samples at levels of 150-450 and 30-72 ng g(-1), respectively, as it provides results similar to those obtained by ashing the sample. However, the typically low level of Co in these samples precluded its determination by the proposed method (the study was made in an SRM spiked wholemeal flour), at least in those samples that were contaminated with elevated concentrations of the metal (viz. more than 90 ng of Co per g of flour). The method provides a relative standard deviation of 6, 8, and 4% for Ni, Cr, and Co, respectively.

The aim of this work was to evaluate current in-house HPLC procedures for the determination of naturally-occurring folates in food, and to identify problem areas for further improvement. Five intercomparison studies were completed over the period 1990–1997 in which nine participants from six countries took part. Through careful validations and detailed discussions held at evaluation meetings, possible biases and sources of systematic error have been identified and reduced. The use of ascorbic acid and nitrogen flushing during extraction, sample clean-up using strong anion exchange columns, spectrophometrically calibrated standards and fluorescence detection are all recommended. Both in-house hog kidney and human plasma deconjugase enzymes gave similar results to the circulated common hog kidney enzyme which was prepared from fresh pig’s kidneys. The most consistently reported values were for 5-CH3H4-PteGlu, and to a lesser extent, for H4PteGlu. Four candidate reference materials (CRM 121, wholemeal flour; CRM 421, milk powder; CRM 485, lyophilised mixed vegetables, and CRM 487, lyophilised pig’s liver) have been proposed with both indicative values (mean ± uncertainty) for 5-CH3H4-PteGlu in CRM 421 (0.25; ± 0.02 mg/kg) and CRM 485 (2.14; ±0.42 mg/kg), and information values (mean; range) for 5-CH3H4-PteGlu in CRM 121 (0.04; 0.03–0.08 mg/kg) and CRM 487 (2.6; 1.9–3.8 mg/kg). Certified values are also given for total folate by microbiological assay: CRM 121 (0.50; ±0.07 mg/kg), CRM 421 (1.42; ±0.14 mg/kg), CRM 485 (3.15; 0.28 mg/kg), and CRM 487 (13.4; 1.3 mg/kg). Average recovery of 5-CH3H4-PteGlu, added prior to extraction and deconjugation, was 91% (84–95%) for the four CRMs. The average within- and between-laboratory variations were 6 and 15% for the determination of 5-CH3H4-PteGlu by HPLC, and 9 and 18% for the determination of total folate by microbiological assay. These CRMs will be used for quality control of folate measurements for nutritional labelling, and validation of new techniques. Further methodology work is required for the HPLC analyses of folate forms other than 5-CH3H4-PteGlu.1

The rheological behaviour of tarhana, a popular Turkish fermented wheat flour–yoghurt soup, was studied at different temperatures (30 to 70 °C). Results showed that an increase in shear rate resulted in a decrease in apparent viscosity, suggesting pseudoplastic behaviour at all temperatures studied. A power law equation was used to calculate the flow behaviour indices, n , ranging from 0.52 to 0.87. The n values were higher for the soups made from wholemeal flour than obtained for the soups made from white wheat flour. Consistency indices decreased with temperature and were described by the Arrhenius equation with activation energies from 48.6 to 28.6 kJ/mol.

The purpose of the project was to study how conventional and ecological farming systems and different dough kneading intensity affected the baking properties of wholemeal flour, and how those properties affected the taste and consistency of wholemeal bread. Sensory evaluations were performed with respect to wholemeal tin loaves from winter wheat. The dough from each wheat sample was divided into two parts. One part was subjected to low kneading intensity, the other to high kneading intensity. High kneading intensity refers to standard commercial practices. Wholemeal from the conventional farming system had a higher protein content than wholemeal from ecological farming systems. Wholemeal from the conventional farming system resulted in bread with a large volume and a high degree of elasticity while wholemeal from ecological farming systems resulted in a dry bread. High kneading intensity generally resulted in a dry and less elastic bread which had a significantly stronger tinge of grey on the surface of the slice.

The aim of this study was to determine crude protein, fi bre and phytic acid in vitro digestibility of selected legumes and buckwheat products. All analyses except the phytic acid contents were performed in the line with the Commission Regulation (EC) No. 152/2009. A modifi ed version of Holt's Method was used for phytic acid (phytate) determination. None of all samples contained more than 11% of moisture. Soybeans are rich in crude protein; they contain nearly 40% of this compound. The content of crude protein in buckwheat fl ours was about 14%. The highest amount of phytate was found in common beans and soybeans-about 2 g/100 g of dry matter. On the other hand, the lowest phytate content was observed in buckwheat pasta (< 1 g/100 g). The quantifi cation of phytate in F. esculentum groats was 1.9 g per 100 g of dry matter. In vitro digestibility was determined using an incubator Daisy and pepsin enzymes and the combination of pepsin and pancreatin. The highest coeffi cient of crude protein digestibility was discovered to be in peels and wholemeal fl our. The greatest fi bre digestibility coeffi cients were obtained for peels, which contain about 65% of fi bre in their dry matter. When pepsin was used, a higher phytic acid digestibility coeffi cient for G. max, Ph. vulgaris, peels, fl our, groats and broken groats was observed; while when the combination of pepsin and pancreatin was used, higher phytic acid digestibility coeffi cients for peas, lentil and wholemeal fl our were observed.

Bread is a staple food worldwide. It is a good source of energy, protein, dietary fibre (DF), minerals, vitamins and many other bioactive compounds. Increased intake of DF has been associated with a decreased risk of overweight and obesity, hypertension, cardiovascular diseases, diabetes, certain cancers and constipation. The use of refined flour in bread decreases its content of DF and associated bioactive compounds. Therefore wholemeal flour, composite flour, bran, resistant starch, fructan/fructo-oligosaccharides or fibre concentrates have been added to bread to enrich it with DF. However, these ingredients can modify many properties of the dough and the bread, so the bread-making process has to be adjusted to achieve desirable products. Many processes can result in the loss of functionality and positive health effects of the DF components, an important aspect to consider when creating innovative products.

No difference in wheat phytase activity was observed when different types of acid were used to adjust the pH of wholemeal wheat flour suspensions to pH 5·0, the optimum for wheat phytase. When whole wheat bread was made without additives or after adjustment of the dough pH with acetic acid or lingonberry (traditional ingredients in bread making in Sweden), 64%, 96% and 83%, respectively, of the initial phytate was hydrolysed. A small but significant difference between breads with and without yeast or with deactivated yeast was found, indicating that yeast contributed some phytase activity under the conditions of bread making (pH 5·3–5·8 and 30–37°C). The optimum pH of yeast phytase was found to be 3·5. The isomers of IP 5 formed with purified wheat phytase or yeast phytase were studied using sodium phytate as substrate. Wheat phytase formed 1,2,3,4,5-IP 5 whereas yeast phytase formed 1,2,4,5,6-IP 5 . Determination of the isomers of inositol pentaphosphate demonstrated that the reduction in phytate levels in bread compared with wholemeal flour resulted from both wheat and yeast phytase activities.

Samples of sound home-grown wheat (one hard and one soft milling) were obtained, cleaned, and gamma-irradiation used to reduce numbers of viable naturally-occurring fungi. Each sample was inoculated with a toxigenic strain of Penicillium verrucosum and monitored for ochratoxin A formation. When ochratoxin A had reached a level of 60 micrograms/kg, the samples were milled into ten fractions which were analysed for ochratoxin A by an HPLC method with immunoaffinity column clean-up. Each straight-run white flour was baked into bread which was analysed in the same way. Relationships between ochratoxin A levels in naturally-contaminated wheat and the products of milling and baking were established. The recovery of ochratoxin A in wholemeal compared with the cleaned wheat was essentially complete and no significant loss occurred on baking white or wholemeal flour into bread. Recoveries in the straight-run white flours, however, were only approximately one-third for the hard wheat and two-thirds for the soft wheat of the ochratoxin A in the uncleaned wheat. The reason for this was that a much higher proportion of the ochratoxin A was found in the bran and offal fractions from hard wheat than from soft. Conversely, a much higher proportion of the ochratoxin A was found in the reduction flour from soft wheat than from hard. Scouring was examined as a possible method of decontamination of wheat prior to milling. This process removes a proportion of the pericarp (bran coat) prior to milling. The results of the study confirmed that scouring reduced the ochratoxin A level in white and wholemeal flour three-fold for both the hard and soft wheat.

Simultaneous Determination of Vitamins.--Klejdus et al. described a simultaneous determination of 10 water- and 10 fat-soluble vitamins in pharmaceutical preparations by liquid chromatography-diode-array detection (LC-DAD). A combined isocratic and linear gradient allowed separation of vitamins in 3 distinct groups: polar, low-polar, and nonpolar. The method was applied to pharmaceutical preparations, fortified powdered drinks, and food samples, for which results were in good agreement with values claimed. Heudi et al. described a separation of 9 water-soluble vitamins by LC-UV. The method was applied for the quantification of vitamins in polyvitaminated premixes used for the fortification of infant nutrition products. The repeatability of the method was evaluated at different concentration levels and coefficients of variation were 45% for other foods. Mean total folate ranged from 14 to 279 microg/100 g for a mixed vegetable reference material, from 5 to 70 microg/100 g for strawberries, and from 28 to 81 microg/100 g for wholemeal flour. One should realize a large variation in results, which might be caused by slight modifications in the microbiological analysis of total folate in foods or the analysis in various (unfortified) food matrixes. Furthermore, optimal combination of enzymes and reaction conditions may vary depending on the composition of the food. Padrangi and Laborde showed recently that treatment with alpha-amylase had no significant effect on measured folate in spinach, although addition of protease significantly increased the release of folate. LC/MS applications gain increasing attention because of their specificity. Rychlik used stable isotope dilution assays for the determination of the folate content of broccoli and bread. Compared to data in the literature and food data bases, amounts were significantly lower. Pawlosky et al., however, found comparable values for 5-methyltetrahydrofolic acid and folic acid by HPLC analysis with fluorescent detection and HPLC/MS. Among samples analyzed were CRMs and broccoli. Besides folic acid, other water-soluble vitamins were also determined by LC/MS/MS by Leporati et al. The method was applied to the quantitative analysis of the natural content of vitamins in typical Italian pasta samples, as well as in fortified pasta samples produced for the U.S. market. Biotin.-A paper from Staggs et al. included the assertion that existing biotin data in food composition tables are inaccurate because the majority are based on bioassays with all relevant disadvantages. Data in most cases are overestimated with consequences for recommendations for dietary biotin intake. An HPLC/avidin-binding assay was used to analyze 87 foods to support the hypothesis mentioned.

Malt extract is used for improving the bread quality made from flour with low ability to form sugars. Introduced in dough it is useful for the yeast development, and multiplication and improve the gas fermentation yield. Therefore malt extract is considered a dough improver. An important role is played by proteolytic enzymes of malt extracts. Present in large amounts proteolytic enzymes lead to worsening the bread quality and increase dough property to widen. Therefore for improving quality of malt extract, must take into account not only the chemical components but also the fermentation activity. Dosing of malt extract is made depending on its fermentation activity and the properties flour, so the amount varies between 1 and 3% by weight flour.The purpose of this study was to emphasize the positive changes of the physical and chemical properties of wholemeal bread, by adding malt extract in different percentages (1% and 2%)

A laboratory method was evaluated for the production of tarhana, a popular Turkish fermented wheat-yogurt mixture. The changes in pH, the total titratable acidity and the vitamin content (thiamine, riboflavin and vitamin B12) of tarhana of different formulations (type of wheat flour, amount of yogurt and presence of salt) were monitored during fermentation. Chemical composition and acceptability of laboratory-produced tarhana were compared with those of authentic homemade and commercially produced tarhana. The pH and titratable acidity of tarhana samples did not change after the third day in the course of a 4-day fermentation. The final pH and acidity (expressed as percent lactic acid) of tarhana were found to be in the range of 4.3–4.8 and 1.8–2.3 (dry basis), respectively. The thiamine, riboflavin and vitamin B12 contents of tarhana did not change considerably during fermentation. The addition of salt to tarhana lowered the acid formation rate during fermentation, leading to a higher pH. The replacement of white wheat flour with wholemeal flour resulted in an expected increase in the protein and vitamin content of tarhana; however, a decrease in overall acceptability was observed.

Arabinoxylans are considered as the major limiting factor for use of rye in animal feeding. In this study, four selected winter rye cultivars were grown at nine locations and tested for arabinoxylans including total (TAX), soluble (SAX) and insoluble fractions (IAX), extract viscosity, falling numbers and crude protein contents. Amounts of TAX ranged on average between 89 and 103 g/kg wholemeal flour, and the extract viscosity between 2.6 and 5.1 cSt. The extract viscosity was significantly correlated with falling numbers and all three arabinoxylan fractions. Arabinoxylan contents and extract viscosity were influenced by the rye genotype. The anthocyanin free hybrid cultivar Helltop combined reduced TAX, SAX and IAX levels with low extract viscosity and high crude protein contents and seems to be more suitable for use in animal feeding than the other three cultivars tested in this respect.

A laboratory protocol was developed to assess glucose and ethanol yields from wheat. The impact of the analyzed wholemeal flour quantity and the saccharification on the amount of released glucose was estimated. The whole process including the analytical methods (glucose and ethanol) was repeatable and reproducible. This protocol was used to assess the glucose and ethanol yields of six varieties and of a complete set of hexaploid near-isogenic waxy lines of cv. Tremie grown in three locations. As compared to the normal line of Tremie, double null (AnBnD) and triple null (nAnBnD) isogenic lines showed a low Hagberg falling number (218, 65, and 63 s, respectively), a higher grain protein content (10.7, 11.5, and 12.1% DM, respectively), a lower glucose yield (728, 703, and 707 kg/t, respectively), and a lower ethanol yield (463, 453, and 452 L/t, respectively). These values indicate a strong involvement of alleles encoded at Wx-B1 and Wx-D1 loci in grain composition.

The effect of cooking on proteins from acha and durum wheat was assessed from an analysis of protein extractability, gel electrophoretic profiles, in-vitro protein digestibility (IVPD) and the amino acid compositions of wholemeal flour and residue proteins. Heating wholemeal flour samples at 100-140 degrees C (t = 10-40 min) resulted in 0-30% and 45-55% decreases in acha and durum protein solubility, respectively. In general, high molecular weight (30-70 kDa) protein subunits were more susceptible to heat damage. For both cereals, sodium dodecyl sulphate (SDS; 10 g litre-1) and/or dithiothreitol (DTT; 10mM) increased protein solubility in unheated and heated samples. The IVPD index was 90-91% and was not significantly altered by cooking (100-120 degrees C, t = 40 min). Cooking at extreme temperatures (140 degrees C, t = 40 min) reduced the IVPD by 8% (P = 0.05). Osborne fractionation resulted in a durum or acha residue level of 7.8% or 55.2%. Treatment with solvent containing propanol, SDS and/or DTT at room temperature followed by SDS-polyacrylamide gel electrophoresis of non-solubilised proteins showed that the glutelin fraction of acha, with the exception of a 65 kDa subunit, was insoluble owing to strong inter-subunit hydrophobic and disulphide interactions. Wholemeal acha flour and residue protein showed a significantly greater level of hydrophobic and sulphur amino acids as well as glutamine which is associated with H-bonding. The possibility that cereal protein solubility is also dependent on protein-carbohydrate links is discussed.

The influence of wheat flour type on the production of flavour compounds in wheat sourdoughs was investigated in sourdoughs fermented with four strains of lactic acid bacteria. The acidification of the sourdoughs and the production of lactic acid were influenced mainly by the flour type, with most lactic acid produced in sourdoughs made from low-grade flour and wholemeal flours. The production of volatile flavour compounds, including acetic acid, in the sourdoughs depended primarily on the starter culture used. An interaction between the starter culture and the flour type was observed for some of the flavour compounds, however. The flour type had a significant effect on the production of ethyl acetate and ethanol in sourdoughs fermented with heterofermentative cultures, with the highest amounts detected in sourdoughs made from wholemeal flour and low-grade flour.

Near-infrared reflectance (NIR) spectroscopy was used in the characterization of grain morphology mutants of barley ( Hordeum vulgare L.) in relation to grain nitrogen (N) content and protein composition. Derivative spectroscopy provided spectra with enhanced resolution, allowing wavelengths to be identified with clear differences in contribution from associated chemical bonds. Comparisons of fourth-derivative spectra of wholemeal flour from high-N grains with flour from low-N grains identified wavelengths at which there were statistically significant differences between the groups. Their importance was independently confirmed by step-up regression using these wavelengths to generate an equation predicting N content (R(2) = 0.98). Fourth-derivative spectral comparisons also allowed novel biochemical differences to be predicted. Visual assessment of the spectra of all mutants revealed a variable region (1470-1520 nm, corresponding to N-H stretch vibrations) that allowed two extreme sets to be defined. The protein extracted from these two sets differed markedly in hordein content.

Flour and dough quality are very important breeding traits for wheat (Triticum aestivum L) the selection of which would benefit from linked molecular markers. Association analysis allows the identification of genes or quantitative trait loci (QTL) related to target traits. The effectiveness of this approach depends on the availability of a densely genotyped population with large genetic variability for the traits of interest. Our objective was to identify chromosomal regions associated with flour and dough quality traits using a core collection built by maximizing the genetic diversity and phenotypic variability. We investigated the association of DArT, SSR and SNP markers with grain protein content, grain hardness, water-extractable arabinoxylan (WEAX) viscosity, wholemeal flour color and dough rheology. Out of 803 markers tested, 130 markers spread over the genome were associated with at least one trait, varying from 20 associated with WEAX viscosity to 60 associated with dough quality. Most chromosome regions known to determine wheat quality from the study of several bi-parental recombinant inbred lines were confirmed here and some new regions were identified. Associations were found for each trait studied, demonstrating that the core collection is a useful tool for QTL association mapping of these and other traits of interest. (C) 2011 Elsevier Ltd. All rights reserved.