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Mittels Infrarotthermografie können Veränderungen der Oberflächentemperatur der Haut dargestellt werden. Kraftbeanspruchungen führen zur Wärmeproduktion in der belasteten Muskulatur. Diese erzeugte Wärme wird zur Hautoberfläche weitergeleitet und daraus resultierende Veränderungen der Hauttemperatur können durch den Einsatz der Infrarotthermografie visualisiert und quantifiziert werden. Die vorliegende kumulativ angefertigte Arbeit umfasst Beiträge, die wesentliche Einflussfaktoren auf belastungsinduzierte Hauttemperaturveränderungen nach Kraftbeanspruchungen aufzeigen. Dazu wurden in einer Studie der Einfluss des Belastungsfaktors Trainingsintensität und in einer weiteren Untersuchung wesentliche Faktoren der Körperkomposition überprüft. Eine dritte Untersuchung zeigt einen Ansatz für eine effiziente automatische Messmethode zur Bestimmung der Hauttemperaturen mittels Fusion einer Infrarotkamera mit einer Tiefenbildkamera. Die Arbeit leistet somit einen Beitrag für die Anwendung der Infrarotthermografie im leistungsphysiologischen Kontext.

Fluorescence microscopy is an indispensable tool for biology to study the spatio-temporal dynamics of cells, tissues, and developing organisms. Modern imaging modalities, such as light-sheet microscopy, are able to acquire large three- dimensional volumes with high spatio-temporal resolution for many hours or days, thereby routinely generating Terabytes of image data in a single experiment. The quality of these images, however, is limited by the optics of the microscope, the signal-to-noise ratio of acquisitions, the photo-toxic effects of illumination, and the distortion of light by the sample. Additionally, the serial operation mode of most microscopy experiments, where large data sets are first acquired and only afterwards inspected and analyzed, excludes the possibility to optimize image quality during acquisition by automatically adapting the microscope parameters. These limits make certain observations difficult or impossible, forcing trade-offs between imaging speed, spatial resolution, light exposure, and imaging depth. This thesis is concerned with addressing several of these challenges with computational methods. First, I present methods for visualizing and processing the volumetric data from a microscope in real-time, i.e. at the acquisition rate of typical experiments, which is a prerequisite for the development of adaptive microscopes. I propose a low-discrepancy sampling strategy that enables the seamless display of large data sets during acquisition, investigate real-time compatible denoising, convolution, and deconvolution methods, and introduce a low-rank decomposition strategy for common deblurring tasks. Secondly, I propose a computational tractable method to simulate the interaction of light with realistically large biological tissues by combining a GPU-accelerated beam propagation method with a novel multiplexing scheme. I demonstrate that this approach enables to rigorously simulate the wave-optical image formation in light-sheet microscopes, t

To analyze the involvement of rheumatoid factors (RF) in the generation of cryoglobulins and the development of related tissue injuries, we have established a panel of anti-IgG2a RF mAbs derived from MRL/MpJ-lpr/lpr (MRL-lpr), C3H/HeJ-lpr/lpr, and 129/Sv mice. After injection of hybridoma cells to normal mice, all four IgG3 RF mAbs induced cryoglobullnemia, and various degrees of glomerulonephritis and skin leukocytoclastic vasculitis. In contrast, none of the RF mAbs of the other isotypes generated cryoglobulins or tissue lesions. Since the same observation was obtained with another panel of five clonally related anti-IgG2a RF mAbs of MRL-lpr origin with almost Identical heavy and light chain variable (V) regions but five different Isotypes, it seems unlikely that the absence of pathogenicity of non-IgG3 RF mAbs was due to differences in fine specificity or V framework regions. In addition, the analysis of serum RF In MRL-lpr mice has demonstrated that a majority of 4 month old MRL-lpr mice produced substantial amounts of IgG3 RF with cryoglobulin activity. Because the cryoglobulin activity is associated with the murine IgG3 heavy chain constant region, RF of this subclass may play a significant role in the development of autoimmune-related tissue injuries, especially In MRL-lpr mice