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While chemical synapses are very plastic and modifiable by defined activity patterns, gap junctions, which mediate electrical transmission, have been classically perceived as passive intercellular channels. Excitatory transmission between auditory afferents and the goldfish Mauthner cell is mediated by coexisting gap junctions and glutamatergic synapses. Although an increased intracellular Ca2+ concentration is expected to reduce gap junctional conductance, both components of the synaptic response were instead enhanced by postsynaptic increases in Ca2+ concentration, produced by patterned synaptic activity or intradendritic Ca2+ injections. The synaptically induced potentiations were blocked by intradendritic injection of KN-93, a Ca2+/calmodulin-dependent kinase (CaM-K) inhibitor, or CaM-KIINtide, a potent and specific peptide inhibitor of CaM-KII, whereas the responses were potentiated by injection of an activated form of CaM-KII. The striking similarities of the mechanisms reported here with those proposed for long-term potentiation of mammalian glutamatergic synapses suggest that gap junctions are also similarly regulated and indicate a primary role for CaM-KII in shaping and regulating interneuronal communication, regardless of its modality.

The recovery from prior stimulation of the compound action potential (CAP) was measured using a forward masking stimulus paradigm in four normal-hearing, human subjects. The CAP was recorded using a wick electrode placed on the tympanic membrane. The effects of a 4000-Hz, 97-dB SPL conditioning stimulus on CAP amplitude in response to a 4000-Hz probe were measured as a function of conditioner-probe interval for three probe levels. The normalized probe response amplitude was completely recovered to the control values at an average conditioner-probe interval of 1359 ms, similar to that observed in chinchilla (Relkin, E.M., Doucet, J.R., Sterns, A., 1995. Recovery of the compound action potential following prior stimulation: evidence for a slow component that reflects recovery of low spontaneous-rate auditory neurons, Hear. Res. 83, 183-189). The present results are interpreted as a consequence of the slow recovery of low spontaneous-rate (SR), high threshold neurons from prior stimulation (Relkin, E.M., Doucet, J.R., 1991. Recovery from prior stimulation. I: Relationship to spontaneous firing rates of primary auditory neurons. Hear. Res. 55, 215-222) and may provide indirect physiological evidence for the existence of a class of low-SR auditory neurons in humans.

Our preceding paper described SPL-dependent changes in the shape of transfer functions recorded from inner and outer hair cells as well as supporting cells, in the 500-2500 Hz regions of the Mongolian gerbil cochlea. As SPL was increased, large shifts were observed in the peak of the transfer function. A strongly compressive nonlinearity was also observed at CF. This paper examines the data from the perspective of intensity coding in the auditory periphery. Based on the data, we offer a new explanation for the mechanisms underlying the different dynamic ranges of low and high threshold auditory neurons. We also find that, for pure tone stimuli, the growth of excitation at the characteristic place saturates rapidly, and cannot encode the wide dynamic range of loudness. The data are analyzed to explore other excitation pattern candidates for loudness coding. The growth of the peak of the IHC transfer function, as well as the growth of the response area, have been found to be linearly related to loudness growth over most of its dynamic range. Implications of the data for auditory intensity coding are discussed.

To determine the transient frequency and intensity sensitivities of central auditory neurons, we implemented an exponential sweep tone stimulus (2 sec in period, mean sweep rate 3.3 octave/sec), intensity of which varied systematically across trials. Response of single units to the stimulus was studied at the inferior colliculus (IC) of urethane-anesthetized rats. Most IC units responded to the sweep tone by one or more transient increases in discharge rate. The area of increased discharge, or response area (RA), was delineated on the frequency-intensity plane. The tip of RA gives the best frequency (BF) and minimum threshold (MT) of the cell. We also compared the BF and MT concurrently obtained with another method, viz., the conventional 'audio-visual' method of subjective judgment. Results showed that for the same population of cells (n=130), correlation between the two methods is better for BF (r=0.91) than for MT (r=0.78). Such discrepancy was discussed in relation to the response characteristics of these central auditory neurons.

1. Developmental changes in amplitude and time course of single-fibre-evoked and spontaneous EPSCs mediated by AMPA and NMDA receptors at the endbulb-bushy cell synapse of rats from 4 to 22 days of age were recorded using whole-cell patch-clamp methods in in vitro slices of cochlear nucleus. 2. The mean conductance of the AMPA component of evoked EPSCs increased by 66 %, while that of the NMDA component decreased by 61 %, for 12- to 18-day-old rats cf. 4- to 11-day-old rats. 3. The mean AMPA spontaneous EPSC conductance increased by 54 %, while mean NMDA spontaneous EPSC conductance decreased by 83 %, for 12- to 22-day-old rats cf. 4- to 11-day-old rats. The mean number of quanta contributing to peak evoked AMPA conductance also increased by 78 % in the older age group, after correction for the asynchrony of evoked quantal release. 4. The decay time constant of spontaneous AMPA EPSCs showed a small decrease in older animals, while the decay time constant of spontaneous NMDA EPSCs was markedly decreased in older animals. The decay time constants of evoked NMDA EPSCs showed a quantitatively similar decrease to that of spontaneous NMDA EPSCs. This suggests that AMPA receptor subunit composition is unlikely to undergo developmental change, while NMDA receptor subunit composition may be substantially altered during synaptic maturation. 5. These data are consistent with a developmentally increased efficacy of AMPA receptor-mediated synaptic transmission at the endbulb-bushy cell synapse, due to an increase in underlying AMPA-mediated quantal size and content during the same period as a transient co-localization of NMDA receptors.

During an investigation of the effects of cochlear cooling on frequency tuning and input/output relations of single auditory nerve fibers in gerbil (Ohlemiller and Siegel (1994) Hear. Res. 80, 174-190), cooling-related changes in post-stimulus time histogram (PSTH) shape and phase-locking to tonebursts were characterized in a small sample of neurons. Local cochlear cooling by 5-10 degrees C below normal core temperature did not alter overall PSTH shape, although some evidence was found for a reduction in the time constants of rapid and short term rate adaptation. The relative contributions of rapid and short term response components appeared unaltered. Effects of cooling on phase-locking were assessed by calculating the synchronization index for responses to intense ( > 70 dB SPL) tonebursts at 0.5, 1.0, and 2.0 kHz. Synchronization filter functions exhibited modest reductions in both magnitude and the upper frequency limit of phase-locking. The effects of cooling on the temporal character of responses appear distinct from those of a simple reduction in stimulus intensity. Results are interpreted in terms of cooling-related changes in responses of cochlear hair cells and afferent neurons, and suggest that temperature artifacts are unlikely to underlie reported species differences in PSTH shape and phase-locking.

The vowel "eh" was used to study auditory-nerve responses at high sound levels (60-110 dB). By changing the playback sampling rate of the stimulus, the second formant (F2) frequency was set at best frequency (BF) for fibers with BFs between 1 and 3 kHz. For vowel stimuli, auditory-nerve fibers tend to phase-lock to the formant component nearest the fiber's BF. The responses of fibers with BFs near F2 are captured by the F2 component, meaning that fibers respond as if the stimulus consisted only of the F2 component. These narrowband responses are seen up to levels of 80-100 dB, above which a response to F1 emerges. The F1 response grows, at the expense of the F2 response, and is dominant at the highest levels. The level at which the F1 response appears is BF dependent and is higher at lower BFs. This effect appears to be suppression of the F2 response by F1. At levels near 100 dB, a component 1/component 2 transition is observed. All components of the vowel undergo the transition simultaneously, as judged by the 180 degrees phase inversion that occurs at the C2 transition. Above the C2 threshold, a broadband response to many components of the vowel is observed. These results demonstrate that the neural representation of speech in normal ears is degraded at high sound levels, such as those used in hearing aids.

The distortion-product otoacoustic emission (DPOAE) 2f1-f2 was measured in the ear canal of the barn owl. DPOAE were elicited by primary tones in 11 frequency regions from 1 to 9 kHz. The highest DPOAE output levels and best thresholds were found for f1 frequencies of 4 to 7 kHz and additionally at the lowest f1 frequency investigated. In some cases, the DPOAE sound pressures were only 37 dB below the primary-tone levels (PTL). The optimal primary-tone frequency ratios ranged from 1.05 to 1.45 and varied strongly among the different frequency regions investigated. The largest optimal ratios were measured in the middle frequency range for f1. At lower and higher f1, the optimal ratios decreased. DPOAE levels could be suppressed in a frequency-selective way by adding a third tone. As in other non-mammals, the best suppressive frequencies were near f1, suggesting DPOAE generation near the frequency place of this primary tone. This is in contrast to what is known for mammalian species, where the DPOAE is thought to be generated near f2. To obtain 6 dB of suppression of the DPOAE level, suppressor-tone levels ranging from 13 dB below to 4 dB above the primary-tone level were necessary. The Q10dB-values of suppression tuning curves increased as a function of frequency up to a value of 15.8. This tendency resembled the increase in frequency selectivity of auditory nerve fibers in this species.

Potassium channels are involved in the control of neuronal excitability by fixing the membrane potential, shaping the action potential, and setting firing rates. Recently, attention has been focused on identifying the factors influencing excitability in second-order auditory and vestibular neurons. Located in the brainstem, second-order auditory and vestibular neurons are sites for convergence of inputs from first-order auditory or vestibular ganglionic cells with other sensory systems and also motor areas. Typically, second-order auditory neurons exhibit two distinct firing patterns in response to depolarization: tonic, with a repetitive firing of action potentials, and phasic, characterized by only one or a few action potentials. In contrast, all mature vestibular second-order neurons fire tonically on depolarization. Already, certain fundamental roles have emerged for potassium currents in these neurons. In mature auditory and vestibular neurons, I(K), the delayed rectifier, is required for the fast repolarization of action potentials. In tonically firing auditory neurons, I(A), the transient outward rectifier, defines the discharge pattern. I(DS), a delayed rectifier-like current distinguished by its low threshold of activation, is found in phasically firing auditory and some developing vestibular neurons where it limits firing to one or a few spikes, and also may contribute to forming short-duration excitatory postsynaptic potential (EPSPs). Also, I(DS) sets the threshold for action potential generation rather high, which may prevent spontaneous discharge in phasically firing cells. During development, there is a gradual acquisition and loss of some potassium conductances, suggesting developmental regulation. As there are similarities in membrane properties of second-order auditory and vestibular neurons, investigations on firing pattern and its underlying mechanisms in one system should help to uncover fundamental properties of the other.

Extracellular recordings were made with microelectrodes from single neurons in the rat's dorsal nucleus of the lateral lemniscus (DNLL) and response characteristics were determined for monaural and binaural acoustic stimulation. The vast majority of DNLL neurons were narrowly tuned to sound frequency and their temporal responses to contralateral tone pulses fell into one of three broad categories: onset (57%), sustained (21%) or onset-pause-sustained (22%). Most DNLL neurons fired multiple action potentials to a single click delivered to the contralateral ear. The majority (77%) of DNLL neurons showed a monotonic increase in the number of spikes elicited by contralateral tone pulses of increasing sound pressure level; the remaining cells were weakly non-monotonic. No obvious tonotopic pattern was found in the distribution of characteristic frequency of neurons in DNLL. Most DNLL neurons exhibited either excitatory/inhibitory (74%) or excitatory/excitatory (9%) binaural response patterns. The remaining cells (17%) were monaural and driven exclusively by stimulation of the contralateral ear. The binaural neurons in DNLL were sensitive to both interaural intensity and interaural time differences as determined by presentation of dichotic tone bursts and clicks respectively. The responses of DNLL neurons could be distinguished on the basis of monaural and binaural response characteristics from those in surrounding areas including the sagulum, paralemniscal zone and the intermediate nucleus of the lateral lemniscus.

Acoustic signals are extensively used for guiding various behaviors in frogs such as vocalization and phonotaxis. While numerous studies have investigated the anatomy and physiology of the auditory system, our knowledge of intrinsic properties and connectivity of individual auditory neurons remains poor. Moreover, the neural basis of audiomotor integration still has to be elucidated. We determined basic response patterns, dendritic arborization and axonal projection patterns of auditory midbrain units with intracellular recording and staining techniques in an isolated brain preparation. The subnuclei of the torus semicircularis subserve different tasks. The principal nucleus, the main target of the ascending auditory input, has mostly intrinsic neurons, i.e., their dendrites and axons are restricted to the torus itself. In contrast, neurons of the magnocellular and the laminar nucleus project to various auditory and non-auditory processing centers. The projection targets include thalamus, tegmentum, periaqueductal gray, medulla oblongata, and in the case of laminar neurons--the spinal cord. Additionally, tegmental cells receive direct auditory input and project to various targets, including the spinal cord. Our data imply that both auditory and premotor functions are implemented in individual toral and tegmental neurons. Their axons constitute parallel descending pathways to several effector systems and might be part of the neural substrate for differential audiomotor integration.

Inhibitory inputs from nucleus reticularis thalami and the inferior colliculus activate gamma-aminobutyric acid B (GABA(B)) receptors in auditory thalamic neurons. These metabotropic receptors have been implicated in the oscillatory behavior of thalamic neurons. We studied the effects of the GABA(B) receptor agonist, baclofen, on membrane and filter properties of neurons in the ventral partition of the medial geniculate body (MGBv) of the rat, using whole-cell patch-clamp recording techniques in a slice preparation. Application of baclofen caused a concentration-dependent and reversible hyperpolarization of MGBv neurons. An increase in membrane conductance shunted voltage signals. The shunt suppressed firing in both tonic and burst modes which normally characterize the neuronal excitation from depolarized and hyperpolarized potentials, respectively. The GABA(B) receptor antagonist, CGP 35348 (0.5 mM), completely and reversibly blocked the baclofen-evoked hyperpolarization and increase in conductance. In voltage-clamp and during blockade of synaptic transmission with tetrodotoxin and Cd2+, baclofen activated an inwardly rectifying outward K+ current, that was sensitive to blockade with Ba2+ (0.5 mM). Intracellular applications of GTPgammaS occluded the baclofen current whereas similar applications of GDPbetaS prevented it, suggesting that G-proteins mediated the baclofen current. We measured the impedance amplitude profile in the frequency domain with swept sinusoidal current injection. MGBv neurons normally have lowpass filter characteristics at depolarized potentials and resonance at approximately 1 Hz at hyperpolarized potentials. Baclofen application reduced the impedance below 20 Hz which lowered the membrane filter quality and abolished the resonance. Despite its hyperpolarizing effect, therefore, baclofen eliminated an intrinsic tendency to oscillate as well as the intrinsic frequency selectivity of MGBv neurons.

The effect of interpulse intervals on the perception of loudness of biphasic current pulse trains was investigated in eight adult cochlear implantees at three different stimulus levels encompassing the psychophysical dynamic range. Equal-loudness contours and thresholds were obtained for stimuli in which two biphasic pulses were presented in a fixed repetition period (4 and 20 ms), and also for single-pulse/period stimuli with rates varying between 20 and 750 Hz. All stimuli were of 500-ms duration, and the phase durations of each pulse were 100 microseconds or less. The results of these experiments were consistent with predictions of a three-stage model of loudness perception, consisting of a peripheral refractory effect function, a sliding central integration time window, and a central equal-loudness decision device. Application of the model to the data allowed the estimation of neural refractory characteristics of the subjects' remaining peripheral neural population. The average neural spike probability for a 50-Hz stimulus was predicted to be about 0.77, with an associated neural refractory time of 7.3 ms. These predictions did not vary systematically with level, implying that the effect of increasing current level on loudness results more from recruitment of neurons than from any increase in average spike probability.

Auditory neurons tuned to the direction and rate of frequency modulations (FM) might underlie the encoding of frequency sweeps in animal vocalizations and formant transitions in human speech. We examined the relationship between FM direction and rate selectivity and the precise temporal interactions of excitatory and inhibitory sideband inputs. Extracellular single-unit recordings were made in the auditory midbrains of eight mustached bats. Up- and down-sweeping linear FM stimuli were presented at different modulation rates in order to determine FM selectivity. Brief tone pairs with varying interstimulus delays were presented in a forward masking paradigm to examine the relative timing of excitatory and inhibitory inputs. In the 33 units for which tone pair data were collected, a correspondence existed between FM rate selectivity and the time delays between paired tones. Moreover, FM directional selectivity was strongly linked to rate selectivity, because directional preferences were expressed only at certain rates and not others. We discuss how abnormalities in the relative timing of inputs could alter or abolish the selectivity of such neurons, and how such a mechanism could account for the perceptual deficits for formant transitions seen in certain children with phonological deficits.

Long-term potentiation (LTP), the increase in synaptic strength evoked by high-frequency stimulation, is often considered to be a cellular model for learning and memory. The validity of this model depends on the assumptions that physiological stimuli can induce LTP in vivo and that the resulting synaptic modifications correlate with behavioural changes. However, modifiable synapses are generally embedded deep in complex circuits. In contrast, the goldfish Mauthner (M)-cell and its afferent synapses are easily accessible for electrophysiological studies, and firing of this neuron is sufficient to trigger fast escape behaviour in response to sudden stimuli. We have previously shown that tetanic stimulation can induce LTP of the feedforward inhibitory synapses that control the excitability of the M-cell. Here we report that natural sensory stimulation can induce potentiation of this inhibitory connection that resembles the LTP induced by afferent tetanization. Furthermore, comparable acoustic stimulation produced a parallel decrease in the probability of the sound-evoked escape reflex. Thus we demonstrate for the first time, to our knowledge, a behavioural role for the long-term synaptic strengthening of inhibitory synapses.

Included among the exciting findings in auditory neuroscience are (i) central plasticity after peripheral injury and (ii) regeneration of auditory nerve fibres following excitotoxic damage. The present study extends our understanding of auditory system plasticity by examining changes in peripheral and central physiology as the cochlea recovers from temporary deafferentation due to excitotoxicity. Application of kainic acid (60 mM) to the round window membrane substantially depressed responses from both auditory nerve and brain stem (inferior colliculus), without affecting distortion-product otoacoustic emissions from the inner ear. The auditory nerve input/output functions recovered over a 30-day period whereas recovery of brainstem response amplitudes occurred within five days. In contrast to amplitudes, thresholds at both peripheral and central levels recovered simultaneously, within five days after kainic acid application. The results indicate that (i) cochlear afferent neurons can recover after excitotoxic damage; (ii) response threshold itself, either central or peripheral, is not sufficient to assess the integrity of the auditory periphery; (iii) the central auditory system can recover more rapidly than the periphery; and (iv) the system can maintain its function in the normal range as peripheral function continues to improve.

This paper investigates the spatial resolution of electrical intracochlear stimulation in order to enable further refinement of cochlear implants. For this purpose electrical potential distributions around a conventional human intracochlear electrode (NUCLEUS-22) were measured in a tank, in cat cadaver cochleae and in living cat cochleae. Potential gradients were calculated where of importance. The values were compared to spatial tuning curves from cat primary auditory afferents in electrical mono-, bi-, and various tripolar stimulation modes. Finally, a lumped element model was developed to elucidate the single fiber data. Tank potential measurements show the principal features of the different stimulation modes but are not sufficient to explain all the features of experimental data from single fibers. Intracochlear potential measurements indicate an increase in spatial resolution in an apical direction. The single fiber data also confirm that a tripolar stimulus configuration provides significantly better spatial resolution than any other stimulation mode presently in use.

Objective: This study aimed to illustrate some recent advances in intraoperative monitoring of the seventh and eighth cranial nerves (i.e., direct recording of cochlear nerve action potentials [CNAPs] and facial nerve action potentials [FNAPs])., Study Design: Reports of representative cases and average postoperative results obtained in subjects submitted to cerebellopontine angle surgical procedures are examined. Results obtained in patients with direct recording techniques are compared with those obtained with the classical monitoring techniques (auditory brain stem response [ABR], electrocochleography [ECoG], facial electromyography [EMG])., Setting: The study was conducted at an Ear Nose and Throat Department, University of Verona, Verona, Italy., Intervention: Intraoperative monitoring during cerebellopontine angle surgery was performed., Results: Patients monitored with direct audiomonitoring techniques presented better postoperative auditory functions compared to patients monitored with ABR. The FNAP and EMG groups showed FN outcome that did not differ significantly., Conclusions: Intraoperative monitoring of cranial nerves furnishes a valid tool for identification of neural structures, prevention of damage, understanding of the pathophysiology of damage, and prediction of postoperative function. The fundamental prerequisite for obtaining optimal benefits from monitoring is the use of techniques of direct and continuous electrophysiologic recording with instantaneous feedback to the surgeon, such as CNAPs and FNAPs.

The effects of changes in perilymphatic tonicity on the semicircular canal were investigated by combining the measurements of transepithelial potential and endolymphatic ionic composition in the isolated frog posterior canal with the electrophysiological assessment of synaptic activity and sensory spike firing at the posterior canal in the isolated intact labyrinth. In the isolated posterior canal, the endolymph was replaced by an endolymph-like solution of known composition, in the presence of basolateral perilymph-like solutions of normal (230 mosmol/kg), reduced (105 mosmol/kg, low NaCl) or increased osmolality (550 mosmol/kg, Na-Gluconate added). Altered perilymphatic tonicity did not produce significant changes in endolymphatic ionic concentrations during up to 5 min. In the presence of hypotonic perilymph, decreased osmolality, K and Cl concentrations were observed at 10 min. In the presence of hypertonic perilymph, the endolymphatic osmolality began to increase at 5 min and by 10 min Na concentration had also significantly increased. On decreasing the tonicity of the external solution an immediate decline was observed in transepithelial potential, whereas hypertonicity produced the opposite effect. In the intact frog labyrinth, mEPSPs and spike potentials were recorded from single fibers of the posterior nerve in normal Ringer's (240 mosmol/kg) as well as in solutions with modified tonicity. Hypotonic solutions consistently decreased and hypertonic solutions consistently increased mEPSP and spike frequencies, independent of the species whose concentration was altered. These effects ensued within 1-2 min after the start of perfusion with the test solutions. In particular, when the tonicity was changed by varying Na concentration the mean mEPSP rate was directly related to osmolality. Size histograms of synaptic potentials were well described by single log-normal distribution functions under all experimental conditions. Hypotonic solutions (105 mosmol/kg) markedly shifted the histograms to the left. Hypertonic solutions (380-550 mosmol/kg, NaCl or Na-Gluconate added) shifted the histograms to the right. Hypertonic solutions obtained by adding sucrose to normal Ringer's solution (final osmolality 550 mosmol/kg) increased mEPSP and spike rates, but did not display appreciable effects on mEPSP size. All effects on spike discharge and on mEPSP rate and size were rapidly reversible. In Ca-free, 10 mM EGTA, Ringer's solution, the sensory discharge was completely abolished and did not recover on making the solution hypertonic. These results indicate that perilymphatic solutions with altered tonicity produce small and slowly ensuing changes in the transepithelial parameters which may indirectly affect the sensory discharge rate, whereas relevant, early and reversible effects occur at the cytoneural junction. In particular, the modulation of mEPSP amplitude appears to be postsynaptic; the presynaptic effect on mEPSP rate of occurrence is presumably linked to local calcium levels, in agreement with previous results indicating that calcium inflow is required to sustain basal transmitter release in this preparation.

When responses to one part of a sequence of auditory signals reduce the responses to a subsequent portion of the signal, "forward masking" results. Although forward masking occurs in the auditory nerve, that observed in the ventral cochlear nucleus (VCN) more closely resembles psychophysical forward masking. In contrast to the auditory nerve in which the amount of forward masking is proportional to the amount of excitation produced by the masker, most VCN neurons show a poor correlation between forward masking and excitation produced by the masker, indicating a more complex interaction between responses to adjacent signals. This study tested the hypothesis that one component of forward masking is produced by inputs from centrifugal neural connections to the VCN. The centrifugal pathways were interrupted with knife-cut lesions medial to the CN. Responses of single units obtained 60 minutes after the lesions were compared to those obtained before the lesions. In primarylike, sustained chopper and on units the lesions resulted in a reduction in forward masking and enhanced recovery. In contrast, lesions resulted in increased masking in primarylike-notch and low-intensity chopper units. The relationship between masker-elicited excitation and forward masking became more monotonic for transient choppers and on units, approaching that observed for auditory nerve fibers. These effects are probably the result of removal of both inhibitory and excitatory inputs, ultimately reflecting a balance of excitation and inhibition to each neural population in the VCN.

Electric charge has long been hypothesized to be the effective stimulus variable that determines loudness evoked by directly stimulating the auditory nerve. This 'equal-charge, equal-loudness' hypothesis predicts that stimulus amplitude and duration can be traded linearly to produce equal loudness. Loudness sensations from threshold to maximum loudness were measured systematically as a function of stimulus amplitude and duration in cochlear implant listeners. The measured data do not support the equal-charge, equal-loudness hypothesis: an increment in stimulus amplitude produces a significantly louder sensation than the same change in stimulus duration. Instead of the linear equal-charge model, a power-function model successfully predicts the measured data and should be used to encode loudness in electric hearing.

The acquisition of adult-like frequency selectivity is generally assumed to be the tightly coupled to improvements in threshold sensitivity during cochlear development. In this study, frequency versus threshold (tuning) curves obtained from 1108 auditory-nerve fibers were used to investigate the relationship between tuning and threshold at characteristic frequency (CF) during postnatal development in kittens. At the earliest ages included in this study, sharpness was within the adult range, but thresholds were significantly higher than adult values. Tuning and thresholds improved along different exponential time courses that varied with CF. For units with CFs below 1 kHz, tuning curve slopes below CF matured earliest, followed by CF threshold, and then by slopes above CF. In contrast, for CFs above 1 kHz, the high-frequency slopes matured first, followed by threshold and then by slope below CF. One interpretation of these results is that tuning and thresholds are not tightly coupled in immature animals. Paradoxically, however, high-frequency slopes were correlated with threshold for individual units at all ages, suggesting that the relationship between tuning and threshold is maintained during development. This contradiction can be resolved by a developmental model that features a functional separation between cochlear nonlinearities and mechanical/electrical conversion.

The purpose of this study was to model the adult human's scalp recorded evoked response to auditory pulses separated by varying inter pulse intervals (IPIs). The responses modeled probably reflect auditory nerve and brainstem generators. The subjects were 10 young adult humans with normal hearing. They were presented pseudo random sequences of pulses (maximum length sequences, MLSs) in order to characterize their system response. For the stimuli and the responses modeled accounting for temporal nonlinearities (interactions among the pulses) improved model performance only marginally. Nonlinear contributions to the models decreased with increasing interval between the input pulses. Increasing the memory of the model beyond 20 ms did not increase modeled performance dramatically. Model performance varied as a function of minimum IPI (MIPI) of the MLSs. At the shortest MIPI overall model performance deteriorated (due, in part, to a decrease in SNR), but nonlinear effects became relatively more important. At the longest MIPI performance also deteriorated, possibly due to the increasing influence of longer latency, more variable evoked potential components. Modeled performance generalized to responses recorded in the same recording session to the same and different MLSs. This study confirms the similarity between MLS linear kernels and conventionally averaged evoked responses--both are adapted responses reflecting the IPIs of the evoking stimuli.

The olivocochlear bundle (OCB) was cut in neonatal cats to evaluate its role in the development of normal cochlear function. Approximately 1 year after deefferentation, acute auditory nerve fiber (ANF) recordings were made from lesioned animals, lesion shams, and normal controls. The degree of deefferentation was quantified via light microscopic evaluation of the density of OCB fascicles in the tunnel of Corti, and selected cases were analyzed via electron microscopy. In the most successful cases, the deefferentation was virtually complete. ANFs from successfully lesioned animals exhibited significant pathophysiology compared with normals and with other animals in which the surgery failed to interrupt the OCB. Thresholds at the characteristic frequency (CF), the frequency at which ANFs are most sensitive, were elevated across the CF range, with maximal effects for CFs in the 10 kHz region. Frequency threshold or tuning curves displayed reduction of tip-to-tail ratios (the difference between CF and low-frequency "tail" thresholds) and decreased sharpness of tuning. These pathological changes are generally associated with outer hair cell (OHC) damage. However, light microscopic histological analysis showed minimal hair cell loss and no significant differences between normal and deefferented groups. Spontaneous discharge rates (SRs) were lower than normal; however, those fibers with the highest SRs remained more sensitive than those with lower SRs. Findings suggest that the interaction between OC efferents and OHCs early in development may be critical for full expression of active mechanical processes.

Objective: This study aimed to compare recordings of the electrically evoked whole nerve action potential (EAP) made using the reverse telemetry system of the Nucleus CI24M device with those recorded from individuals who use the Ineraid cochlear implant system., Study Design: Data were collected in a prospective fashion from Nucleus CI24M cochlear implant users and compared with retrospective data collected from patients who use the Ineraid device., Setting: All data were collected at the Department of Otolaryngology-Head and Neck Surgery, University of Iowa Hospitals and Clinics., Patients: Data are reported from 8 patients who use the Nucleus CI24M cochlear implant and 20 patients who use the Ineraid cochlear implant system., Interventions: The interventions described in this study were diagnostic in nature., Main Outcome Measures: EAP growth and refractory recovery data are reported. EAP thresholds recorded from patients who use the Nucleus CI24M device also are compared with behavioral thresholds for the stimulus used to evoke the EAP as well as the stimulation levels needed to program the speech processor., Results: EAP morphology, growth, and refractory recovery functions recorded using the Nucleus CI24M reverse telemetry system compared favorably with similar measures recorded from Ineraid cochlear implant users., Conclusions: Reasonable EAP responses can be recorded using the Nucleus CI24M device. More data are needed to determine whether the information about neural responsiveness available with this device will be clinically useful.

We recorded electrically evoked compound action potentials (EAPs) from guinea pigs and cats using monophasic current pulses delivered by a monopolar intracochlear electrode. By using simple stimuli, we sought results that could shed light on basic excitation properties of the auditory nerve. In these acute experiments, the recording electrode was placed directly on the auditory nerve. Responses to anodic and cathodic stimulus pulses were recorded separately to evaluate stimulus polarity effects. Several polarity-dependent properties were observed. Both EAP morphology and latency were polarity-dependent, with greater latencies for cathodic stimulation. Threshold stimulus level was also polarity-dependent, but in different directions in the two species: cats had lower cathodic thresholds while guinea pigs had lower anodic thresholds. We also observed that the slopes of the EAP amplitude-level functions depended upon stimulus polarity. In most cases where EAP saturation amplitude could be measured, that amplitude was similar for anodic and cathodic stimuli, suggesting that either stimulus polarity can recruit all fibers, or at least a comparable numbers of fibers. The common findings (e.g., EAP morphology and polarity-dependent latency) observed in these two species suggest results that can be extrapolated to responses obtained in humans, while the species-specific findings (e.g., dependence of threshold on polarity) may point to underlying anatomical differences that caution against overgeneralization across species. Some of our observations also bear upon hypotheses of how electrical stimuli may excite different sites on auditory nerve fibers.

Responses to free-field sound of 401 fibers from the VIIIth nerve of the grassfrog, Rana temporaria, are described. The spontaneous activities of the fibers ranged from 0 to 75 spikes/s, showing only weak correlation with frequency or sensitivity of the fibers. The highest spontaneous activities were approximately twice as high as reported previously for frogs. Best frequencies ranged from 100 to 1600 Hz and thresholds ranged from 21 to 80 dB SPL. The median dynamic range was 20 dB and the slopes of the rate-level curves ranged from 5 to 20 spikes/(s-dB). Most of the units showed post-excitatory suppression (PS) of their spontaneous activity. The duration of PS increased with sound level, also in fibers showing a decrease in firing rate at high intensities. Most fibers showing one-tone suppression did not show PS at their best suppression frequencies. Strong suppression was observed also in very phasic cells giving one spike per stimulation. Therefore, the mechanism underlying PS is probably different from that underlying adaptation. The sharpening of the neural encoding of temporal parameters and the strong encoding of sound offset as well as onset caused by PS very likely is biologically important.

The purpose of the present study was to measure the change in threshold as a function of stimulus duration in single auditory nerve fibers. Thresholds were measured at each neuron's characteristic frequency (CF) for eight stimulus durations ranging from 8 to 1024 ms. Using an adaptive, two-interval, forced-choice threshold-tracking procedure with a 2-down, 1-up rule, thresholds were estimated based on a decision criterion of one spike or greater difference between tone and no-tone intervals. The results showed that mean thresholds decreased with increasing stimulus duration by approximately 14.6 dB over the range of durations tested. Analysis of group and individual data showed that thresholds decreased by approximately 6-7 dB per decade of duration. The slope of threshold improvement decreased systematically with increasing CF, consistent with previous physiological and psychophysical data.

Transient linear acceleration stimuli have been shown to elicit eighth nerve vestibular compound action potentials in birds and mammals. The present study was undertaken to better define the nature of the adequate stimulus for neurons generating the response in the chicken (Gallus domesticus). In particular, the study evaluated the question of whether the neurons studied are most sensitive to the maximum level of linear acceleration achieved or to the rate of change in acceleration (da/dt, or jerk). To do this, vestibular response thresholds were measured as a function of stimulus onset slope. Traditional computer signal averaging was used to record responses to pulsed linear acceleration stimuli. Stimulus onset slope was systematically varied. Acceleration thresholds decreased with increasing stimulus onset slope (decreasing stimulus rise time). When stimuli were expressed in units of jerk (g/ms), thresholds were virtually constant for all stimulus rise times. Moreover, stimuli having identical jerk magnitudes but widely varying peak acceleration levels produced virtually identical responses. Vestibular response thresholds, latencies and amplitudes appear to be determined strictly by stimulus jerk magnitudes. Stimulus attributes such as peak acceleration or rise time alone do not provide sufficient information to predict response parameter quantities. Indeed, the major response parameters were shown to be virtually independent of peak acceleration levels or rise time when these stimulus features were isolated and considered separately. It is concluded that the neurons generating short latency vestibular evoked potentials do so as "jerk encoders" in the chicken. Primary afferents classified as "irregular", and which traditionally fall into the broad category of "dynamic" or "phasic" neurons, would seem to be the most likely candidates for the neural generators of short latency vestibular compound action potentials.

Computational models of the peripheral auditory system have largely modeled basilar membrane (BM) mechanics as a linear filter-bank-like entity. Recent mathematical work on the nature of auditory system noise suppression allows us to analyze and argue for the incorporation of BM nonlinearities into these models. This analysis shows that vowel perception improves with increasing presence of BM nonlinearities whereas consonant perception degrades with increasing influence of BM nonlinearities. Experimental results on tones and real speech corrupted by noise corroborate the analysis as well as suggest a novel approach to speech processing.

Far-field recordings of short latency vestibular responses to pulsed cranial translation are composed of a series of positive and negative peaks occurring within 10 ms following stimulus onset. In the bird, these vestibular evoked potentials (VsEPs) can be recorded noninvasively and have been shown in the chicken and quail to depend strictly upon the activation of the vestibular component of the eighth nerve. The utility of the VsEP in the study of vestibular systems is dependent upon a clear understanding of the neural sources of response components. The primary aim of the current research in the chicken was to critically test the hypotheses that 1) responses are generated by both peripheral and central neurons and 2) peaks P1 and N1 originate from first order vestibular neurons, whereas later waves primarily depend on activity in higher order neurons. The principal strategy used here was to surgically isolate the eighth nerve as it enters the brainstem. Interruption of primary afferents of the eighth nerve in the brainstem substantially reduced or eliminated peaks beyond P2, whereas P1 and N1 were generally spared. Surgical sections that spared vestibular pathways had little effect on responses. The degree of change in response components beyond N1 was correlated with the extent of damage to central vestibular relays. These findings support the conclusion that responses are produced by both peripheral and central elements of the vestibular system. Further, response peaks later than N1 appear to be dependent upon central relays, whereas P1 and N1 reflect activity of the peripheral nerve. These findings clarify the roles of peripheral and central neurons in the generation of vestibular evoked potentials and provide the basis for a more useful and detailed interpretation of data from vestibular response testing.

Regeneration/repair and protection of auditory hair cells and auditory neurons is an exciting, rapidly evolving field. Simultaneous developments in the fields of otobiology and surgical otology have led to new and exciting possibilities in inner ear medicine and surgery; specifically, the treatment or prevention of a variety of types of hearing losses in the foreseeable future. Sensorineural hearing loss in humans is commonly associated with a loss of auditory hair cells. It has been generally accepted that hearing loss resulting from hair cell damage is irreversible because the human ear has been considered to be incapable of regenerating or repairing these sensory elements following severe injury. An organ of Corti explant study has shown that it is possible to initiate the regeneration/repair of mammalian hair cells. In this study, ototoxin-damaged organ of Corti explants from juvenile rats were treated with a combination of retinoic acid (10-8M) and fetal calf serum (10%). TGF-alpha has been identified as a growth factor capable of evoking auditory hair cell regeneration/repair in ototoxin-damaged organ of Corti explants. Preliminary in vitro experiments with juvenile rat organ of Corti explants and in vivo studies in the cochleae of adult guinea pigs indicate that pretreatment followed by continuous treatment of the inner ear with a combination of retinoic acid and TGF-alpha can protect the auditory hair cells from the ototoxic effects of aminoglycosides. Because the integrity of spiral ganglion neurons is also essential for normal auditory function, there is a parallel series of in vitro and in vivo studies of the effects of neurotrophic factors on the survival of auditory neurons and the regeneration of injured neuronal processes. Clinical studies have demonstrated that it is now possible to perform surgeries on the inner ear, i.e., partial or total labyrinthectomies, and maintain hearing. The field of cochlear implantation has also provided insights into both the short- and long-term effects of cochlear fenestration on inner ear function. Administration of growth factors to the inner ears of animals is now possible with the use of implanted catheters and miniature infusion pumps. These advances suggest that localized application of drugs to the human inner ear may be feasible. The aim of this paper has been to provide an overview of advances in the study of the biology of auditory hair cells and auditory neurons, as well as recent relevant surgical advances. Taken together, these advances in otobiology and surgery will, in the future, be combined to devise new and innovative treatments for inner ear disorders.

Single-unit responses were studied in the ventral cochlear nucleus (VCN) of cats as formant and trough features of the vowel /epsilon/ were shifted in the frequency domain to each unit's best frequency (BF; the frequency of greatest sensitivity). Discharge rates sampled with this spectrum manipulation procedure (SMP) were used to estimate vowel representations provided by populations of VCN neurons. In traditional population measures, a good representation of a vowel's formant structure is based on relatively high discharge rates among units with BFs near high-energy formant features and low rates for units with BFs near low-energy spectral troughs. At most vowel levels and in the presence of background noise, chopper units exhibited formant-to-trough rate differences that were larger than VCN primary-like units and auditory-nerve fibers. By contrast, vowel encoding by primary-like units resembled auditory nerve representations for most stimulus conditions. As is seen in the auditory nerve, primary-like units with low spontaneous rates (SR <18 spikes/s) produced better representations than high SR primary-like units at all but the lowest vowel levels. Awake cats exhibited the same general response properties as anesthetized cats but larger between-subject differences in vowel driven rates. The vowel encoding properties of VCN chopper units support previous interpretations that patterns of auditory nerve convergence on cochlear nucleus neurons compensate for limitations in the dynamic range of peripheral neurons.

Spontaneous otoacoustic emissions (SOAEs) of nearly identical acoustic frequency in both ears are a common observation, but it is unknown if this binaural mirroring effect is random, artefactual, genetic, developmental, or of other origin. The available raw data of all human SOAE surveys were pooled, and the intervals of all possible binaural emission pairs (N = 9555) were listed according to size on the Cent-scale (1 Cent = 1/100 semitone = 1/1200 octave). Statistical analysis showed (1) a slight broad-band mirroring in the 0-100 Cent range (P < 0.05), and (2) a strong narrow-band mirroring (NBM) in the 0-20 Cent range (P < 0.001). Negative results in a detailed SOAE cluster detection program excluded experimental artefacts as causes of NBM. Analysis of the large subgroup of twin data excluded genetic and intrauterine developmental causes. Systemic developmental causes are unrealistic, as 20 Cent corresponds to only approximately 80 microm on the cochlear map. Analysis of infant data indicated that the effect may be introduced after birth by secondary factors. Interaural crosstalk was examined but had to be rejected. It is suggested that bilaterally spreading period information in the medial olivocochlear system influences outer hair cells of the same best frequency in both ears very similarly. Evidence concerning possible effects on electromotility is discussed, and experimental tests are proposed.

In the cochlea, outer hair cells (OHCs) generate the active cochlear mechanics whereas the supporting cells, such as Deiters' cells and Hensen's cells, may play a role in both the active and passive cochlear mechanics. The presence of receptors for adenosine triphosphate (ATP) on OHCs, Deiters' cells and Hensen's cells indicates that endogenous ATP may have a role in cochlear mechanics. To explore this possibility, the effects of the ATP antagonist, pyridoxal-phosphate-6-azophenyl-2',4'-disulfonic acid (PPADS), were studied in guinea pig both in vitro on isolated OHCs, Deiters' cells, Hensen's cells and pillar cells using the whole-cell configuration of the patch-clamp technique, and in vivo on sound evoked cochlear potentials (cochlear microphonic, CM; summating potential, SP; compound action potential, CAP) and distortion product otoacoustic emissions (DPOAEs) using cochlear perilymphatic perfusion. Results show that PPADS (100 microM) reduced the inward current evoked by 5-10 microM ATP in OHCs, Deiters' cells, Hensen's cells and pillar cells. This effect of PPADS was slow in onset and was slowly reversed to a varying degree in the different cell types. In vivo application of PPADS in increasing concentrations reduced the sound evoked CAP, SP and increased N1 latency starting at about 0.33 mM (SP) and 1 mM (CAP and N1 latency). PPADS (0.33-1 mM) reversibly suppressed the initial value of the quadratic DPOAE and reversed the 'slow decline' in the quadratic DPOAE that occurs during continuous stimulation with moderate level primaries. These results, together with the similar effects of the ATP antagonist suramin reported previously (Skellett et al., 1997), may be evidence that endogenous ATP acting on cells in the organ of Corti alters cochlear mechanics.

Responses of auditory nerve fibers to trains of clicks were recorded in ketamine anesthetized chinchillas. By varying the number of clicks and the interclick interval, this study examined whether "post-onset adaptation," described in psychoacoustic experiments on localization, occurred in auditory nerve fibers. The results showed that the number of action potentials recorded from a nerve fiber in response to a train of clicks was a power function of the number of clicks. For interclick intervals of 2 ms or greater the exponent of the power function was 0.5, and this exponent did not change over a 20-dB range of intensities. The timing of action potentials relative to the click stimuli was measured using synchronization coefficients. The coefficients increased with interclick interval, decreased with increasing intensity, and were greater for fibers with low rates of spontaneous activity than for high spontaneous fibers. Recovery functions showed that for interclick intervals of 2 ms or more, the responses to the second click were at least 70% of the response to the initial click. The recovery depended upon the number of clicks in the train. These findings indicate that auditory nerve fibers respond to high rates of stimulus presentation and do not display the adaptation observed in localization studies.

Purpose: Facial nerve monitoring is often used to predict postoperative facial function after acoustic neuroma tumor removal. In this study, three methods of predicting facial nerve function were compared. These methods used various parameters of the evoked electromyographic monitoring., Materials and Methods: Thirty-four patients who underwent surgery for acoustic neuroma were retrospectively reviewed. Amplitude of ongoing electromyographic activity, stimulation current thresholds, and the amplitude of evoked response were analyzed. The predictive value of the three methods was compared with actual postoperative facial nerve function., Results: One method predicted the final postoperative facial function in 90% of the patients, one method in 84%, and the final method failed to predict the final VIIth nerve function in patients with current stimulation thresholds greater than 0.05 mA., Conclusion: Analysis of prognostic value showed that one of the three studied proved superior in predicting facial nerve function.

Responses of the principal unit types in the ventral cochlear nucleus of the chinchilla were studied with a single-formant stimulus set that covered fundamental frequency (f0) from 100 Hz to 200 Hz and formant center frequency (F1) from 256 to 782 Hz. Temporal coding for f0 and F1 was explored for 95 stimulus combinations of f0 (n = 5) and F1 (n = 19) in primarylike, onset and chopper unit categories. Several analyses that explored temporal coding were employed including: autocorrelation, interspike interval analysis, and synchronization to each harmonic of f0. In general, the representation of f0 is better in onset and chopper units than in primarylike units. Nearly all units in the cochlear nucleus showed a gain in phase locking to the envelope (f0) of the single-formant stimulus relative to the auditory nerve. The fundamental is represented directly in neural discharges of units in the cochlear nucleus with an interval code (also Cariani and Delgutte, 1996; Rhode, 1995). The formant is represented in the temporal domain in primarylike units, though some chopper and onset units also possess the ability to code F1 through discharge synchrony. Onset-I units, which are associated with the octopus cells, exhibited the strongest phase locking to f0 of any unit types studied. The representation of f0 and F1 in the temporal domain is weak or absent in some units. All-order-interspike interval distributions computed for populations of units show preservation of temporal coding for both f0 and F1. Results are in agreement with earlier amplitude modulation studies that showed nearly all cochlear nucleus unit types phase lock to the signal envelope better than auditory nerve fibers over a considerable range of signal amplitudes.

A model based on the potassium current pathway through the hair cell is used to analyze the electrical behavior of mammalian inner and outer hair cells. Without taking into account the effects of calcium it is possible to simulate experimental results concerning the shape and strength of the receptor potential and the frequency dependent ac (alternating current) and dc (direct current) components of the receptor current. This model and a simplified form of it are utilized to explain: (1) Transduction latencies: that the receptor potential follows a stimulating signal with a very short delay, under the assumption of a constant number of open K+ channels in the lateral part of the cell membrane. (2) Transduction gains: why higher potential changes are measured in inner hair cells than in outer hair cells, although the outer hair cells are expected to be exposed to higher stereociliary motions: in inner hair cells a decrease in the conductance of the basolateral membrane causes higher gain (receptor potential increases) and together with an increase of membrane capacitance slower reaction (a larger time constant). (3) Transduction channel kinetics: that the shortest (0.1 ms) as well as the longest (20 ms) possible open times of the transduction channels in the stereocilia have different frequency related effects on the shape of the receptor potentials.

Temporal response patterns of single auditory nerve fibers were used to characterize the effects of a common hearing-aid processing scheme, frequency-shaped amplification, on the encoding of the vowel /epsilon/ in cats with a permanent noise-induced hearing loss. These responses were contrasted with responses to unmodified stimuli in control and impaired cats. Noise-induced hearing loss leads to a degraded representation of the formant frequencies, in which strong phase locking to the formants is not observed in fibers with best frequencies (BFs) near the formants and there is a wide spread of formant phase locking to fibers with higher BFs (Miller et al., 1997a,b). Frequency shaping effectively limits the upward spread of locking to F1, which improves the representation of higher frequency components of the vowel. However, it also increases phase locking to harmonics in the trough between the formants, which decreases the contrast between F1 and the trough in the neural representation. Moreover, it does not prevent the spread to higher BFs of responses to the second and third formants. The results show a beneficial effect of frequency shaping, but also show that interactions between particular gain functions and particular spectral shapes can result in unwanted distortions of the neural representation of the signal.

Safe electrical stimulation of neural tissue is typically achieved using charge-balanced biphasic current pulses, which are designed to minimize the generation of direct current (DC) and the production of harmful electrochemical products. However, due to the kinetics of the charge injection process, neural stimulators must also use capacitive coupling or electrode shorting techniques, to ensure DC levels are minimal. Previous studies have reported a reduction in excitability of the auditory nerve following acute simulation at high rates and intensities. Elevated levels of DC were reported in these studies despite using charge-balanced biphasic pulses and electrode shorting. The present study was designed to investigate the extent to which DC contributed to these stimulus induced reductions in auditory nerve excitability. Adult guinea pigs were bilaterally implanted and unilaterally stimulated for two hours using charge-balanced biphasic current pulses and stimulus rates of 200, 400 or 1000 pulses/s (pps) at a stimulus intensity well above clinical levels (0.34 microC/phase). DC levels were controlled using either electrode shorting, or electrode shorting with capacitive coupling. Electrically evoked auditory brainstem responses (EABRs) were recorded before and periodically following the acute stimulation. It was found that the extent of reduction in the EABR amplitude was a function of stimulus rate. While there was little change in the EABR following stimulation at 200 pps, significant post-stimulus reductions in the EABR amplitude were observed at stimulus rates of 400 and 1000 pps during the three hour post-stimulus monitoring period. Stimulation using capacitively coupled electrodes, which eliminated all DCs, showed reductions in EABR amplitudes similar to those observed following stimulation using electrode shorting alone. While there was no significant difference in the extent of reduction in EABR amplitudes for capacitive coupling versus electrode shorting at stimulus rates of 200 pps (P > 0.05) and 400 pps (P > 0.05), there was a significant difference at 1000 pps (P< 0.001). The present findings indicate that the major component of the stimulus induced reductions observed in auditory nerve excitability appear to be associated with stimulus induced neuronal activity, although elevated levels of DC ( > 2.5 microA) can also contribute to these changes. However, although statistically significant, the effects of DC are very small compared to the effects of high rate, high intensity stimulation per se.

Low-frequency cells in the anteroventral cochlear nucleus (AVCN) can be sensitive to changes in the spatiotemporal pattern of discharges across their auditory nerve (AN) inputs (). This sensitivity suggests that these cells may be tuned to particular spatiotemporal patterns, or features, in the discharge patterns of populations of AN fibers. To evaluate and characterize this sensitivity, we developed a technique whereby the physiological responses of AVCN cells to wide-band noise were analyzed using the simulated response of a population of AN fibers to the same noise stimulus. By averaging the simulated two-dimensional spatiotemporal pattern of AN activity that preceded each AVCN discharge, it was possible to derive a two-dimensional reverse-correlation function that characterized the spatiotemporal tuning of each AVCN cell. The derived spatiotemporal tuning pattern represented a feature in the AN population response that was most likely to precede discharges of the AVCN cell. To test the spatiotemporal tuning characterizations, we used these patterns to predict the responses of cells to noise stimuli statistically independent from the stimuli used to characterize the cells. This technique provides a general tool for the study of any neural system that involves the analysis of spatiotemporal input patterns.

Essential to improve the performance with cochlear implants is a better control of the electrode-nerve interface. The long-term stability of the electrode characteristics has to be guaranteed. Most important herein is to have a low and stable electrical impedance and to prevent fibrosis. Results of electrode coating and fibrosis-inhibiting products are described. To assess the properties of the electrode-nerve interface such as stimulation threshold, spatial selectivity and channel interaction, the electrically evoked compound action potential is measured. The measurement paradigm and the first results are described. Models of auditory nerve response to electrical stimulation can be used as a tool for cochlear implant fitting. A simple model is proposed and its predictions are compared with measured responses.

It is proposed that two-tone suppression of rate responses in auditory-nerve fibres by a low-side suppressor cannot be explained in terms of basilar membrane motion. In a model, the amplitude of the mechanical response, either to the tone at characteristic frequency (CF), or to the CF tone combined with a second, lower frequency tone (a suppressor), is taken as the effective stimulus to inner hair cells (IHC), the voltage response of which is considered responsible for excitatory drive to auditory-nerve fibres. Many empirical mechanical and physiological effects are simulated accurately by the model, particularly phenomena observed in two-tone experiments using low-side suppressor tones, that authors have described as two-tone suppression. It is argued in this paper, however, that such phenomena strictly do not constitute suppression in the cochlear response and provide no explanation for rate suppression in nerve fibres. According to the model presented here and consistent with experimental data, suppression of the spike response to a CF tone in an auditory-nerve fibre by a low-side suppressor cannot be explained in terms of the mechanics of the BM. Conclusions by others that experiments support a mechanical explanation for low-side rate suppression are shown to be questionable. It is concluded that low-side suppression of neural responses is explicable only in terms of a non-mechanical factor derived from the response to the low frequency tone, that depresses responsiveness in fibres at the CF location. Adherence to the model of low-side neural rate suppression depending on reduced net mechanical response of the BM is contrary to experimental evidence; furthermore it overlooks a profound influence additional to synaptic drive, that is implied in the shaping of responses in auditory-nerve fibres.

Considerable progress has been made with speech processing for multiple-channel cochlear implants, with a mean open-set CNC word score of 40% for electrical stimulation alone for the Nucleus SPEAK strategy. Further advances should be achieved through the following: beam-forming dual microphone systems for hearing in noise; better reproduction of the coding of sound frequencies; and taking advantage of plasticity in the central auditory pathways in young children. The reproduction of frequency coding will be facilitated with speech processors such as the Nucleus 24 system which can provide higher stimulus rates, and a new generation of electrode arrays where a greater number of electrodes lie close to the spiral ganglion cells.

The article covers experimental data about influence of infrasound on hearing and vestibular apparatus, about body changes linked functionally to this apparatus. The authors revealed 4 zones of response of hearing and vestibular apparatus to infrasound. Infrasound appeared to affect directly nuclei of VIII and X cranial nerves and indirectly other organs and systems linked functionally to vagus nerve.