101. White blood cell segmentation using U-Net and its variants to improve leukemia diagnosis.
- Author
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Joshi, Vivek C., Mehta, Mayuri A., and Kotecha, Ketan
- Abstract
Numerous computer-aided leukemia detection methods have been introduced to overcome the limitations of clinical diagnosis procedures. The precision of computer-aided leukemia detection highly depends on the accurate segmentation of white blood cells (WBCs) from the stained whole slide image (WSI). This paper proposes WBC segmentation from WSI using U-Net and its variants. The major contributions to this paper are as follows. First, WBC segmentation is proposed using U-Net, U-Net++ and three transfer learning-based U-Net models, namely U-Net-VGG16, U-Net-VGG19, and U-Net-ResNet. Second, a comprehensive and comparative experimental analysis of the proposed WBC segmentation approaches is presented using four evaluation parameters, namely Dice coefficient, Intersection over Union (IoU), precision, and recall. Third, WBC segmentation approaches are also evaluated using four loss functions such as binary cross-entropy, focal, Dice, and IoU to identify the most effective loss function for WBC segmentation. Finally, the trained models' ability to perform WBC segmentation is validated to assess their practical applicability. The empirical results reveal that U-Net-VGG16 and U-Net-VGG19 achieve a high Dice coefficient of 90% and IoU of 83%. Besides, U-Net++ achieves high precision and recall of 98% and 70%, respectively. Although the results reveal that transfer learning-based U-Net models perform better, deployment of the trained model shows that the U-Net segments WBC more precisely than transfer learning-based U-Net models. [ABSTRACT FROM AUTHOR]
- Published
- 2025
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