Search

Your search keyword '"Tjäderhane L"' showing total 575 results
Start Over Author "Tjäderhane L"
575 results on '"Tjäderhane L"'

105. 2-Hydroxyisocaproic acid is bactericidal in human dental root canals ex vivo.

Author

Sakko, M., Tjäderhane, L., Sorsa, T., Hietala, P., and Rautemaa, R.

Subjects
*ACIDS, *BACTERICIDAL action, *DENTAL pulp cavities, *CALCIUM hydroxide, *ENTEROCOCCUS faecalis, *BACTERIAL growth, *CHLORHEXIDINE, *DENTINAL tubules, *DISEASES, *THERAPEUTICS
Abstract

Aim To compare the activity of 2-hydroxyisocaproic acid (HICA), calcium hydroxide (Ca(OH)2) and chlorhexidine digluconate (CHG) against Enterococcus faecalis T-75359 (root canal isolate) in the root canals of extracted human teeth. Methodology Bacterial suspensions (108 cfu mL−1) were incubated in root canals with 0.9 mm diameter root blocks ( n = 73) for 21 days. Bacterial penetration into dentine was analysed by the Brown and Brenn method ( n = 5). Canals ( n = 17/group) were medicated with 40% of HICA paste, 40% of Ca(OH)2 paste, 2% of CHG solution or 0.9% of saline solution for 7 days. Samples taken from the inner (first 0.1 mm) and deeper (second 0.1 mm) dentine, and residual roots were cultured in broth for 24 h. Bacterial growth was detected by spectrophotometry (optical density, OD) and confirmed by culture on agar. The OD data were analysed with Kruskal-Wallis and Friedman with Wilcoxon signed-rank test between and within groups, respectively, and agar culture data with Pearson chi-square with Mann-Whitney and Cochran with McNemar tests, respectively ( P < 0.05). Results Bacterial invasion into dentine tubules was confirmed. In deeper dentine, HICA inhibited >90% of bacterial growth in comparison with saline. No bacterial growth was observed in 82-100% of inner and deeper dentine samples. CHG prevented the growth in 88%, Ca(OH)2 in 59-76% and saline in 65-71%, respectively. HICA was significantly more active than Ca(OH)2 ( P = 0.008) in the residual roots. The viability testing on agar showed essentially the same result. Conclusion HICA paste exerted superior activity against E. faecalis and could have potential for root canal medication. [ABSTRACT FROM AUTHOR]

Published
2017
Full Text
View/download PDF

112. Polarity of Mature Human Odontoblasts

Author

Tjäderhane, L., primary, Koivumäki, S., additional, Pääkkönen, V., additional, Ilvesaro, J., additional, Soini, Y., additional, Salo, T., additional, Metsikkö, K., additional, and Tuukkanen, J., additional

Published
2013
Full Text
View/download PDF

113. Tooth Bleaching Increases Dentinal Protease Activity

Author

Sato, C., primary, Rodrigues, F.A., additional, Garcia, D.M., additional, Vidal, C.M.P., additional, Pashley, D.H., additional, Tjäderhane, L., additional, Carrilho, M.R., additional, Nascimento, F.D., additional, and Tersariol, I.L.S., additional

Published
2012
Full Text
View/download PDF

116. Effect of Phosphoric Acid on the Degradation of Human Dentin Matrix

Author

Tezvergil-Mutluay, A., primary, Mutluay, M., additional, Seseogullari-Dirihan, R., additional, Agee, K.A., additional, Key, W.O., additional, Scheffel, D.L.S., additional, Breschi, L., additional, Mazzoni, A., additional, Tjäderhane, L., additional, Nishitani, Y., additional, Tay, F.R., additional, and Pashley, D.H., additional

Published
2012
Full Text
View/download PDF

120. Chlorhexidine Inhibits the Activity of Dental Cysteine Cathepsins

Author

Scaffa, P.M.C., primary, Vidal, C.M.P., additional, Barros, N., additional, Gesteira, T.F., additional, Carmona, A.K., additional, Breschi, L., additional, Pashley, D.H., additional, Tjäderhane, L., additional, Tersariol, I.L.S., additional, Nascimento, F.D., additional, and Carrilho, M.R., additional

Published
2012
Full Text
View/download PDF

122. Effect of UVA-activated Riboflavin on Dentin Bonding

Author

Cova, A., primary, Breschi, L., additional, Nato, F., additional, Ruggeri, A., additional, Carrilho, M., additional, Tjäderhane, L., additional, Prati, C., additional, Di Lenarda, R., additional, Tay, F.R., additional, Pashley, D.H., additional, and Mazzoni, A., additional

Published
2011
Full Text
View/download PDF

123. Cysteine Cathepsins in Human Carious Dentin

Author

Nascimento, F.D., primary, Minciotti, C.L., additional, Geraldeli, S., additional, Carrilho, M.R., additional, Pashley, D.H., additional, Tay, F.R., additional, Nader, H.B., additional, Salo, T., additional, Tjäderhane, L., additional, and Tersariol, I.L.S., additional

Published
2011
Full Text
View/download PDF

130. Substantivity of chlorhexidine to human dentin

Author

Carrilho, M.R., primary, Sousa, E.N., additional, di Hipolito, V., additional, Carvalho, R.M., additional, Breschi, L., additional, Tjäderhane, L., additional, Mazzoni, A., additional, Tay, F.R., additional, and Pashley, D.H., additional

Published
2010
Full Text
View/download PDF

137. In vivo Preservation of the Hybrid Layer by Chlorhexidine

Author

Carrilho, M.R.O., primary, Geraldeli, S., additional, Tay, F., additional, de Goes, M.F., additional, Carvalho, R.M., additional, Tjäderhane, L., additional, Reis, A.F., additional, Hebling, J., additional, Mazzoni, A., additional, Breschi, L., additional, and Pashley, D., additional

Published
2007
Full Text
View/download PDF

138. Chlorhexidine Preserves Dentin Bond in vitro

Author

Carrilho, M.R.O., primary, Carvalho, R.M., additional, de Goes, M.F., additional, di Hipólito, V., additional, Geraldeli, S., additional, Tay, F.R., additional, Pashley, D.H., additional, and Tjäderhane, L., additional

Published
2007
Full Text
View/download PDF

139. Role of Dentin MMPs in Caries Progression and Bond Stability.

Author

Mazzoni, A., Tjäderhane, L., Checchi, V., Di Lenarda, R., Salo, T., Tay, F.R., Pashley, D.H., and Breschi, L.

Subjects
DENTIN, MATRIX metalloproteinases, DENTAL caries research, DENTAL bonding, CATHEPSINS
Abstract

Dentin can be described as a biological composite with collagen matrix embedded with nanosized hydroxyapatite mineral crystallites. Matrix metalloproteinases (MMPs) and cysteine cathepsins are families of endopeptidases. Enzymes of both families are present in dentin and collectively capable of degrading virtually all extracellular matrix components. This review describes these enzymes and their presence in dentin, mainly focusing on their role in dentin caries pathogenesis and loss of collagen in the adhesive hybrid layer under composite restorations. MMPs and cysteine cathepsins present in saliva, mineralized dentin, and/or dentinal fluid may affect the dentin caries process at the early phases of demineralization. Changes in collagen and noncollagenous protein structure may participate in observed decreases in mechanical properties of caries-affected dentin and reduce the ability of caries-affected dentin to remineralize. These endogenous enzymes also remain entrapped within the hybrid layer during the resin infiltration process, and the acidic bonding agents themselves (irrespective of whether they are etch-and-rinse or self-etch) can activate these endogenous protease proforms. Since resin impregnation is frequently incomplete, denuded collagen matrices associated with free water (which serves as a collagen cleavage reagent for these endogenous hydrolase enzymes) can be enzymatically disrupted, finally contributing to the degradation of the hybrid layer. There are multiple in vitro and in vivo reports showing that the longevity of the adhesive interface is increased when nonspecific enzyme-inhibiting strategies are used. Different chemicals (i.e., chlorhexidine, galardin, and benzalkonium chloride) or collagen cross-linker agents have been successfully employed as therapeutic primers in the bonding procedure. In addition, the incorporation of enzyme inhibitors (i.e., quaternary ammonium methacrylates) into the resin blends has been recently promoted. This review will describe MMP functions in caries and hybrid layer degradation and explore the potential therapeutic role of MMP inhibitors for the development of improved intervention strategies for MMP-related oral diseases. [ABSTRACT FROM PUBLISHER]

Published
2015
Full Text
View/download PDF

149. Abundance of MMPs and Cysteine Cathepsins in Caries-affected Dentin.

Author

Vidal, C.M.P., Tjäderhane, L., Scaffa, P.M., Tersariol, I.L., Pashley, D., Nader, H.B., Nascimento, F.D., and Carrilho, M.R.

Subjects
MATRIX metalloproteinases, CYSTEINE, CATHEPSINS, DENTIN, DENTAL caries, IMMUNOHISTOCHEMISTRY, COLLAGEN, BIOFLUORESCENCE
Abstract

Degradation of dentin matrix components within caries dentin has been correlated with the activity of host-derived proteases, such as matrix metalloproteases (MMPs) and cysteine cathepsins (CTs). Since this relationship has not been fully established, we hypothesized that the abundance of MMPs and CTs in caries-affected dentin must be higher than in intact dentin. To test this premise, we obtained 5 slices (200 µm) from 5 intact teeth and from 5 caries-affected teeth (1 slice/tooth) and individually incubated them with primary antibodies for CT-B, CT-K, MMP-2, or MMP-9. Negative controls were incubated with pre-immune serum. Specimens were washed and re-incubated with the respective fluorescent secondary antibody. Collagen identification, attained by the autofluorescence capture technique, and protease localization were evaluated by multi-photon confocal microscopy. The images were analyzed with ZEN software, which also quantitatively measured the percentages of collagen and protease distribution in dentin compartments. The abundance of the test enzymes was markedly higher in caries-affected than in intact dentin. CT-B exhibited the highest percentage of co-localization with collagen, followed by MMP-9, MMP-2, and CT-K. The high expression of CTs and MMPs in caries-affected teeth indicates that those host-derived enzymes are intensely involved with caries progression. [ABSTRACT FROM PUBLISHER]

Published
2014
Full Text
View/download PDF

150. Sodium Fluoride Inhibits MMP-2 and MMP-9.

Author

Kato, M.T., Bolanho, A., Zarella, B.L., Salo, T., Tjäderhane, L., and Buzalaf, M.A.R.

Subjects
FLUORIDES, MATRIX metalloproteinases, DENTIN, EXTRACELLULAR matrix, TOOTH demineralization, REMINERALIZATION (Teeth), DENTAL caries, SALIVA
Abstract

The importance of fluoride (F) in preventing dental caries by favorably interfering in the demineralization-remineralization processes is well-established, but its ability to inhibit matrix metalloproteinases (MMPs), which could also help to prevent dentin caries, has not been investigated. This study assessed the ability of F to inhibit salivary and purified human gelatinases MMPs-2 and -9. Saliva was collected from 10 healthy individuals. Pooled saliva was centrifuged, and supernatants were incubated for 1 hr at 37°C and subjected to zymography. Sodium fluoride (50-275 ppm F) was added to the incubation buffer. The reversibility of the inhibition of MMPs-2 and -9 by NaF was tested by the addition of NaF (250-5,000 ppm F) to the incubation buffer, after which an additional incubation was performed in the absence of F. F decreased the activities of pro- and active forms of salivary and purified human MMPs in a dose-response manner. Purified gelatinases were completely inhibited by 200 ppm F (IC50 = 100 and 75 ppm F for MMPs-2 and -9, respectively), and salivary MMP-9 by 275 ppm F (IC50 = 200 ppm F). Inhibition was partially reversible at 250-1,500 ppm F, but was irreversible at 5,000 ppm F. This is the first study to describe the ability of NaF to inhibit MMPs completely. [ABSTRACT FROM PUBLISHER]

Published
2014
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results