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426 results on '"Tang, Rupei"'

113. Low molecular weight PEI-grafted carboxyl-modified soybean protein as gene carriers with reduced cytotoxicity and greatly improved transfection in vitro.

Author

Yao, Weijing, Fu, Shengxiang, Yang, Guanqing, Wang, Jun, Wang, Xin, and Tang, Rupei

Subjects
SOY proteins, MOLECULAR weights, CARRIER proteins, GENE transfection, T cells, ACYLATION
Abstract

SA-SP-PEI were synthesized via acylation reaction between carboxyl-modified soybean protein (SA-SP) and branched polyethylenimine (PEI) with molecular weight of 600, 1200, and 1800 Da, and designed as SA-SP-PEI600, SA-SP-PEI1200, and SA-SP-PEI1800, respectively. SA-SP-PEI could effectively condense plasmid DNA into nanoscale polyplexes and protect them from enzymatic digestion. MTT assay revealed that SA-SP-PEI exhibited reduced cytotoxicity on 293 T and SH-SY5Y cells. SA-SP-PEI1800/DNA complexes hold highest transfection efficiency on 293 T and SH-SY5Y cells with or without 10% serum, which was owing to its better serum stable and improved biocompatibility. Such polycationic soybean proteins have great potentials as gene carriers by further optimization. [ABSTRACT FROM AUTHOR]

Published
2019
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120. Low molecular weight polyethylenimine-grafted soybean protein gene carriers with low cytotoxicity and greatly improved transfection in vitro.

Author

Yao, Weijing, Cheng, Xu, Fu, Shengxiang, Yan, Guoqing, Wang, Xin, and Tang, Rupei

Subjects
SOYBEAN, PROTEINS, POLYETHYLENE, MOLECULAR weights, GENE transfection, CELL-mediated cytotoxicity
Abstract

A series of gene carriers (SP-PEI) have been synthesized by acylation reaction between soybean protein and branched polyethylenimine with low molecular weight of 600, 1200 and 1800 Da, and designed as SP-PEI600, SP-PEI1200 and SP-PEI1800, respectively. SP-PEI could effectively condense plasmid DNA into nanoscale polyplexes with size range of 100–200 nm, and exhibited much lower cytotoxicity against 293T and SH-SY5Y cells than that of branched polyethylenimine (25 kDa). In vitro gene transfection demonstrated that SP-PEI/DNA complex displayed increased transfection against 293T and SH-SY5Y cells with the increase of the weight ratio of SP-PEI/DNA complex with or without 10% serum. At weight ratio of 24, SP-PEI1800/DNA polyplexes showed the highest transfection on SH-SY5Y cells, which was almost three folds higher than PEI (25 kDa). Furthermore, these SP-PEIs/DNA polyplexes could effectively transfect 293T and SH-SY5Y cells with or without 10% serum, suggesting their excellent serum tolerance. [ABSTRACT FROM AUTHOR]

Published
2018
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122. Succinyl pullulan-crosslinked carboxymethyl chitosan sponges for potential wound dressing.

Author

Wang, Xin, Zhang, Di, Wang, Jun, Tang, Rupei, Wei, Bing, and Jiang, Qiong

Subjects
SUCCINYLTRANSFERASES, PULLULANASE, CHITOSAN, SURGICAL sponges, WOUND care, THERAPEUTICS
Abstract

The crosslinked succinyl pullulan-carboxymethyl chitosan composite sponges (Pullulan-CMCS) were fabricated for potential wound dressing. The structure and micromorphology of Pullulan-CMCS sponges were investigated by FTIR spectroscopy and SEM. Pullulan-CMCS sponges had lots of porous structures, making it absorb much liquid and quickly reach swelling equilibrium. WVTR results showed that Pullulan-CMCS sponges can reduce the accumulation of wound exudates in a certain degree. Biocompatibility test showed these sponges had no cytotoxicity or hemolytic potential.In vivoexperiments demonstrated that Pullulan-CMCS sponges can effectively heal full-layer wound of skin defects of male ICR mice with accelerated fibroblast proliferation and enhanced epithelial migration. [ABSTRACT FROM AUTHOR]

Published
2017
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123. Folic acid-conjugated soybean protein-based nanoparticles mediate efficient antitumor ability in vitro.

Author

Yao, Weijing, Zha, Qian, Cheng, Xu, Wang, Xin, Wang, Jun, and Tang, Rupei

Subjects
SOY proteins, DRUG resistance in cancer cells, NANOMEDICINE, FOLIC acid, TARGETED drug delivery, MOLECULAR weights, ULTRAVIOLET spectrophotometry
Abstract

In this study, soy protein isolate was hydrolyzed by compound enzymes to give aqueous soy protein with low molecular weights. Folic acid modified and free soy protein nanoparticles were successfully prepared by a desolvation method as target-specific drug delivery, respectively. Ultraviolet spectrophotometry demonstrated that folic acid was successfully grafted onto soy protein. The shape and size of folic acid modified soy protein nanoparticles were detected by transmission electron microscopy, scanning electron microscope, and dynamic light scattering. In addition, a series of characteristics including kinetic stability, pH stability, and time stability were also performed. Doxorubicin was successfully loaded into folic acid modified soy protein nanoparticles, and the encapsulation and loading efficiencies were 96.7% and 23%, respectively. Doxorubicin-loaded folic acid modified soy protein nanoparticles exhibited faster drug release rate than soy protein nanoparticles in PBS solution (pH = 5). The tumor penetration and antitumor experiments were done using three-dimensional multicellular tumor spheroids as the in vitro model. The results proved that folic acid modified soy protein nanoparticles display higher penetration and accumulation than soy protein nanoparticles, therefore possessing efficient growth inhibitory ability against multicellular tumor spheroids. [ABSTRACT FROM AUTHOR]

Published
2017
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149. Interfacial behaviors of PMMA-PEO block copolymers at the air/water interface

Author

Liu Minghua, Tang Rupei, Cheng Caixia, Xi Fu, and Jiao Ti-Feng

Subjects
Langmuir, Morphology (linguistics), Materials science, General Chemistry, Surface pressure, Folding (chemistry), chemistry.chemical_compound, chemistry, Chemical engineering, Transition point, Monolayer, Polymer chemistry, Copolymer, Methyl methacrylate
Abstract

Diblock copolymer of PMMA 291 - b -PEO 114 and triblock copolymer of PMMA 120 - b - PEO 227 - b -PMMA 120 were synthesized and their interfacial properties at the air-water interface were investigated, where both blocks of polyethylene oxide (PEO) and poly(methyl methacrylate) (PMMA) are surface active but the former is soluble in water while the latter is not. Both the block copolymers could form monolayers with two obvious transition regions. The first transition point is around 10 mN/m, which could be assigned to the pancake-brush change of the PEO chains. The other is around 18 mN/m, which could be ascribed to the condensed packing of PMMA. The surface morphological changes during the compression of the Langmuir monolayers are investigated by using the AFM and SEM methods for the films deposited at different surface pressure or molecular areas. At a lower surface pressure, a typical morphology of PEO-containing lipopolymers is observed. Upon compression, sphere-dominant morphologies were observed. While the diblock copolymer is easy to folding, the triblock copolymer is in favor of formation of circular domains through vesiculation.

Published
2005

150. Amphiphilic block copolymers bearing six-membered ortho ester ring in side chains as potential drug carriers: synthesis, characterization, and in vivo toxicity evaluation.

Author

Luo, Shi, Tao, Yangyang, Tang, Rupei, Wang, Rui, Ji, Weihang, Wang, Chun, and Zhao, Youliang

Subjects
AMPHIPHILES, COPOLYMERS, DRUG carriers, CHEMICAL synthesis, DIOXINS, POLYMERIZATION, ESTERS
Abstract

A new type of amphiphilic block copolymers, poly(ethylene glycol)-block-poly(2-methyl-acrylicacid 2-methoxy-5-methyl-[1,3]dioxin-5-ylmethyl ester) (PEG-b-PMME), bearing acid-labile six-membered ortho ester rings in side chains was synthesized by reversible addition–fragmentation chain-transfer polymerization, and the influence of chain length of the hydrophobic PMME block on micelle properties was investigated. The PEG-b-PMME micelles were stable in aqueous buffer at physiological pH with a low critical micelle concentration. Nile Red as a model drug was encapsulated into the micelles to explore the release profiles. The Nile Red-loaded polymeric micelles showed rapid release of Nile Red in weakly acidic environments (pH 5) but slow release under physiological condition (pH 7.4), due to different hydrolysis rate of ortho ester side chains of PEG-b-PMME. The Paclitaxel (PTX)-loaded micelles retained potency in killing lung cancer cells (A549), compared with the free PTX. No obvious toxicity was foundin vitroandin vivoafter intraperitoneal injection of the micelles, which confirms that the PEG-b-PMME micelles with unique acid-labile characteristic have great potential as nano-scaled carriers for drug delivery. [ABSTRACT FROM AUTHOR]

Published
2014
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