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106. Comparison of hemolytic activity of the intermediate subunit of Entamoeba histolytica and Entamoeba dispar lectins

Author

Kato, Kentaro, Makiuchi, Takashi, Cheng, Xunjia, Tachibana, Hiroshi, Kato, Kentaro, Makiuchi, Takashi, Cheng, Xunjia, and Tachibana, Hiroshi

Abstract

Galactose and N-acetyl-D-galactosamine-inhibitable lectin of Entamoeba histolytica has roles in pathogenicity and induction of protective immunity in rodent models of amoebiasis. Recently, the intermediate subunit of the lectin, Igl1, of E. histolytica has been shown to have hemolytic activity. However, the corresponding lectin is also expressed in a non-virulent species, Entamoeba dispar, and another subunit, Igl2, is expressed in the protozoa. Therefore, in this study, we compared the activities of Igl1 and Igl2 subunits from E. histolytica and E. dispar using various regions of recombinant Igl proteins expressed in Escherichia coli. The recombinant E. dispar Igl proteins had comparable hemolytic activities with those of E. histolytica Igl proteins. Furthermore, Igl1 gene-silenced E. histolytica trophozoites showed less hemolytic activity compared with vector-transfected trophozoites, indicating that the expression level of Igl1 protein influences the activity. These results suggest that the lower hemolytic activity in E. dispar compared with E. histolytica reflects the lower expression level of Igl1 in the E. dispar parasite., PLOS ONE, 12(7), e0181864; 2017

Published
2017

112. Cover Picture: One Electron‐Initiated Two‐Electron Oxidation of Water by Aluminum Porphyrins with Earth's Most Abundant Metal (ChemSusChem 9/2017)

Author

Kuttassery, Fazalurahman, primary, Mathew, Siby, additional, Sagawa, Shogo, additional, Remello, Sebastian Nybin, additional, Thomas, Arun, additional, Yamamoto, Daisuke, additional, Onuki, Satomi, additional, Nabetani, Yu, additional, Tachibana, Hiroshi, additional, and Inoue, Haruo, additional

Published
2017
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114. Promotive Effect of Bicarbonate Ion on Two‐Electron Water Oxidation to Form H2O2 Catalyzed by Aluminum Porphyrins.

Author

Kuttassery, Fazalurahman, Sebastian, Abin, Mathew, Siby, Tachibana, Hiroshi, and Inoue, Haruo

Subjects
OXIDATION of water, BICARBONATE ions, CARBONATE minerals, PORPHYRINS, ARTIFICIAL photosynthesis, ALUMINUM oxidation, ALUMINUM
Abstract

Two‐electron water oxidation initiated by one‐electron oxidation of aluminum porphyrins (AlTMPyP) is an alternative water oxidation to the conventional four‐electron pathway and could help to avoid the bottleneck subject of photon‐flux density in artificial photosynthesis. Here, a dramatic enhancement of the reactivity by bicarbonate ion in the two‐electron water oxidation to form H2O2 is reported. An addition of sodium carbonate (Na2CO3) controlled both catalytic current and product selectivity of the two‐electron water oxidation to enhance the activity of AlTMPyP at pH≈10–11. Controlled potential electrolysis experiments at different concentrations of Na2CO3 (10–100 mm) showed that peroxide selectivity was improved up to approximately 73 % by the increase of [Na2CO3] added to the system. The promotion of the reaction cycle was induced by an enhanced dynamic capturing of H2O2 from the hydroperoxy complex of AlTMPyP through an attack of a bicarbonate ion. The detailed electrochemical studies and product selectivity indicated that the bicarbonate ion served as a good cofactor for producing H2O2 from water. At stronger alkaline conditions (pH 12.5), however, a retardative effect of the addition of Na2CO3 on the catalytic reactivity was observed. [ABSTRACT FROM AUTHOR]

Published
2019
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115. Influence of Heterologous Transplant of DNA‐lacking Mitochondria from Entamoeba histolytica on Proliferation of Entamoeba invadens.

Author

Kazama, Makoto, Yoshida, Kazuhiro, Ogiwara, Sanae, Makiuchi, Takashi, and Tachibana, Hiroshi

Subjects
MITOCHONDRIAL DNA, NUCLEAR DNA, MITOCHONDRIAL pathology, MICROINJECTION (Cytology), MITOSOMES
Abstract

In mitochondria, compatibility of proteins encoded in mitochondrial DNA and nuclear DNA is essential for the normal functioning of the organelle. Incompatibility between mitochondrial and nuclear DNA can lead to dysfunctional respiration, mitochondrial diseases, and lethal problems, which suggests that the presence of heterologous mitochondria is unfavorable. In a previous study, we established a transplant method for DNA‐lacking mitochondria (mitosomes) in the anaerobic protozoan Entamoeba histolytica. In this study, interspecies transplant of mitosomes from E. histolytica into Entamoeba invadens, which is a parasitic protozoon of reptiles, was performed using the microinjection method at various temperatures and injection volumes. When E. invadens was used as recipient, it showed higher tolerance to a lower temperature and larger injection volume, in comparison with E. histolytica. After microinjection, donor mitosomes expressing HA‐tag conjugated protein were observed in recipient cells by immunofluorescent staining. The heterologous mitosomes‐injected cells proliferated and growth rate of the microinjected‐cells was similar to that of intact cells. Therefore, we conclude that interspecies transplant of DNA‐lacking mitochondria does not result in incompatibility. [ABSTRACT FROM AUTHOR]

Published
2019
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127. Novel hemagglutinating, hemolytic and cytotoxic activities of the intermediate subunit of Entamoeba histolytica lectin

Author

Kato, Kentaro, Yahata, Kazuhide, Gopal, Dhoubhadel Bhim, Fujii, Yoshito, Tachibana, Hiroshi, Kato, Kentaro, Yahata, Kazuhide, Gopal, Dhoubhadel Bhim, Fujii, Yoshito, and Tachibana, Hiroshi

Abstract

Galactose and N-acetyl-D-galactosamine (Gal/GalNAc) inhibitable lectin of Entamoeba histolytica, a common protozoan parasite, has roles in pathogenicity and induction of protective immunity in mouse models of amoebiasis. The lectin consists of heavy (Hgl), light (Lgl), and intermediate (Igl) subunits. Hgl has lectin activity and Lgl does not, but little is known about the activity of Igl. In this study, we assessed various regions of Igl for hemagglutinating activity using recombinant proteins expressed in Escherichia coli. We identified a weak hemagglutinating activity of the protein. Furthermore, we found novel hemolytic and cytotoxic activities of the lectin, which resided in the carboxy-terminal region of the protein. Antibodies against Igl inhibited the hemolytic activity of Entamoeba histolytica trophozoites. This is the first report showing hemagglutinating, hemolytic and cytotoxic activities of an amoebic molecule, Igl., Scientific Reports, 5, 13901; 2015

Published
2015

131. Prevalence and genotypic diversity of Entamoeba species in inhabitants in Kathmandu, Nepal.

Author

Tachibana, Hiroshi, Feng, Meng, Cheng, Xunjia, Pandey, Basu Dev, Pandey, Kishor, Yanagi, Tetsuo, Wang, Ting, Putaporntip, Chaturong, Jongwutiwes, Somchai, and Sherchand, Jeevan B.

Subjects
*BIODIVERSITY, *GENOTYPES, *ENTAMOEBA, *SHORT tandem repeat analysis, *RNA sequencing, *GENETICS
Abstract

In Nepal, gastrointestinal infections due to parasites including Entamoeba species are common. The main aim of this study was to identify species of Entamoeba using genotypic analysis. The prevalence of Entamoeba infections was examined by PCR in fecal samples from 143 inhabitants living close to wild rhesus macaques in Kathmandu, Nepal. The numbers of positive cases were one (0.7%) for E. histolytica, eight (5.6%) for E. dispar, seven (4.9%) for E. coli, and two (1.4%) for E. chattoni (E. polecki ST2). No infections with E. nuttalli, E. moshkovskii, and E. polecki ST1 were found. In E. dispar, at least seven different genotypes were detected from the eight samples by sequence analysis of tRNA-linked short tandem repeats. Different genotypes were found even in a couple from the same family. This is the first report demonstrating that E. dispar with high genotypic diversity is prevalent, rather than E. histolytica, in Kathmandu, and that zoonotic transmission of E. chattoni from rhesus macaques might occur in the inhabitants. [ABSTRACT FROM AUTHOR]

Published
2018
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132. Naturally acquired IgG antibodies to thrombospondin-related anonymous protein of Plasmodium vivax (PvTRAP) in Thailand predominantly elicit immunological cross-reactivity.

Author

Kosuwin, Rattiporn, Feng, Meng, Makiuchi, Takashi, Putaporntip, Chaturong, Tachibana, Hiroshi, and Jongwutiwes, Somchai

Subjects
THROMBOSPONDINS, IMMUNOGLOBULIN G, IMMUNOGLOBULINS, PLASMODIUM vivax, MALARIA vaccines, PROTEIN metabolism, DNA metabolism, COMPARATIVE studies, ENZYME-linked immunosorbent assay, RESEARCH methodology, MEDICAL cooperation, PROTOZOA, RESEARCH, EVALUATION research
Abstract

Copyright of Tropical Medicine & International Health is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Published
2018
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133. フィールドワークの実践 －建築デザインの変革をめざして－

Author

Wada, Kouichi, Waraji, Takeo, Sato, Masayuki, Ishigaki, Aya, Meshitsuka, Yusuke, Kurakazu, Ryoko, katsumata, hideaki, Kinoshita, Yoshiro, yamazaki, toshihiko, Matsuda, Yuji, Sasao, Tohru, Oosaki, Atsushi, Yanagisawa, Kaname, Oohashi, Sumiko, Sakurai, Noriko, Tachibana, Hiroshi, Ishii, Satoshi, Yamashita, Tetsuro, Takahashi, Daisuke, Mizoue, yuji, Takada, Itaru, Taniguchi, Kumiko, and Kamei, Yasuko

Published
2011

140. Development of an Immunochromatographic Assay Kit Using Fluorescent Silica Nanoparticles for Rapid Diagnosis of Acanthamoeba Keratitis

Author

Toriyama, Koji, primary, Suzuki, Takashi, additional, Inoue, Tomoyuki, additional, Eguchi, Hiroshi, additional, Hoshi, Saichi, additional, Inoue, Yoshitsugu, additional, Aizawa, Hideki, additional, Miyoshi, Kazutomi, additional, Ohkubo, Michio, additional, Hiwatashi, Eiji, additional, Tachibana, Hiroshi, additional, and Ohashi, Yuichi, additional

Published
2015
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142. Serological Surveillance Development for Tropical Infectious Diseases Using Simultaneous Microsphere-Based Multiplex Assays and Finite Mixture Models

Author

Fujii, Yoshito, Kaneko, Satoshi, Nzou, Samson Muuo, Mwau, Matilu, Njenga, Sammy M., Tanigawa, Chihiro, Kimotho, James, Mwangi, Anne Wanjiru, Kiche, Ibrahim, Matsumoto, Sohkichi, Niki, Mamiko, Osada-Oka, Mayuko, Ichinose, Yoshio, Inoue, Manabu, Itoh, Makoto, Tachibana, Hiroshi, Ishii, Kazunari, Tsuboi, Takafumi, Yoshida, Lay Myint, Mondal, Dinesh, Haque, Rashidul, Hamano, Shinjiro, Changoma, Mwatasa, Hoshi, Tomonori, Kamo, Ken-ichi, Karama, Mohamed, Miura, Masashi, Hirayama, Kenji, Fujii, Yoshito, Kaneko, Satoshi, Nzou, Samson Muuo, Mwau, Matilu, Njenga, Sammy M., Tanigawa, Chihiro, Kimotho, James, Mwangi, Anne Wanjiru, Kiche, Ibrahim, Matsumoto, Sohkichi, Niki, Mamiko, Osada-Oka, Mayuko, Ichinose, Yoshio, Inoue, Manabu, Itoh, Makoto, Tachibana, Hiroshi, Ishii, Kazunari, Tsuboi, Takafumi, Yoshida, Lay Myint, Mondal, Dinesh, Haque, Rashidul, Hamano, Shinjiro, Changoma, Mwatasa, Hoshi, Tomonori, Kamo, Ken-ichi, Karama, Mohamed, Miura, Masashi, and Hirayama, Kenji

Abstract

Background:A strategy to combat infectious diseases, including neglected tropical diseases (NTDs), will depend on the development of reliable epidemiological surveillance methods. To establish a simple and practical seroprevalence detection system, we developed a microsphere-based multiplex immunoassay system and evaluated utility using samples obtained in Kenya.Methods:We developed a microsphere-based immuno-assay system to simultaneously measure the individual levels of plasma antibody (IgG) against 8 antigens derived from 6 pathogens: Entamoeba histolytica (C-IgL), Leishmania donovani (KRP42), Toxoplasma gondii (SAG1), Wuchereria bancrofti (SXP1), HIV (gag, gp120 and gp41), and Vibrio cholerae (cholera toxin). The assay system was validated using appropriate control samples. The assay system was applied for 3411 blood samples collected from the general population randomly selected from two health and demographic surveillance system (HDSS) cohorts in the coastal and western regions of Kenya. The immunoassay values distribution for each antigen was mathematically defined by a finite mixture model, and cut-off values were optimized.Findings:Sensitivities and specificities for each antigen ranged between 71 and 100%. Seroprevalences for each pathogen from the Kwale and Mbita HDSS sites (respectively) were as follows: HIV, 3.0% and 20.1%; L. donovani, 12.6% and 17.3%; E. histolytica, 12.8% and 16.6%; and T. gondii, 30.9% and 28.2%. Seroprevalences of W. bancrofti and V. cholerae showed relatively high figures, especially among children. The results might be affected by immunological cross reactions between W. bancrofti-SXP1 and other parasitic infections; and cholera toxin and the enterotoxigenic E. coli (ETEC), respectively.Interpretation:A microsphere-based multi-serological assay system can provide an opportunity to comprehensively grasp epidemiological features for NTDs. By adding pathogens and antigens of interest, optimized made-to-order high-quality programs ca, PLoS Neglected Tropical Diseases, 8(7), e3040; 2014

Published
2014

147. Serological Surveillance Development for Tropical Infectious Diseases Using Simultaneous Microsphere-Based Multiplex Assays and Finite Mixture Models

Author

Fujii, Yoshito, primary, Kaneko, Satoshi, additional, Nzou, Samson Muuo, additional, Mwau, Matilu, additional, Njenga, Sammy M., additional, Tanigawa, Chihiro, additional, Kimotho, James, additional, Mwangi, Anne Wanjiru, additional, Kiche, Ibrahim, additional, Matsumoto, Sohkichi, additional, Niki, Mamiko, additional, Osada-Oka, Mayuko, additional, Ichinose, Yoshio, additional, Inoue, Manabu, additional, Itoh, Makoto, additional, Tachibana, Hiroshi, additional, Ishii, Kazunari, additional, Tsuboi, Takafumi, additional, Yoshida, Lay Myint, additional, Mondal, Dinesh, additional, Haque, Rashidul, additional, Hamano, Shinjiro, additional, Changoma, Mwatasa, additional, Hoshi, Tomonori, additional, Kamo, Ken-ichi, additional, Karama, Mohamed, additional, Miura, Masashi, additional, and Hirayama, Kenji, additional

Published
2014
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