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Dendrobium is an important source of natural components with high medicinal value. While numerous endophytic fungi associated with these plants have been extensively studied, research on endophytic bacteria remains limited. In this study, an improved tissue separation method was used to isolate endophytic bacteria from Dendrobium nobile and enrich the resources of high-quality biocontrol bacteria with both antibacterial and growth-promoting effects. We assessed the inhibitory effect of the isolated bacteria on pathogenic bacteria and fungi using agar diffusion and plate confrontation methods. We further investigated the growth-promoting effect of bacterial suspensions at various concentrations on corn seeds and Dendrobium nobile plantlets. The obtained endophytic bacterium, JC-3jx, isolated for the first time from the stem of Dendrobium nobile, exhibited significant inhibitory activity against the tested indicator bacteria. Morphologic and phylogenetic analysis confirmed the closest similarity of JC-3jx to Paenibacillus peoriae, leading to its final identification as Paenibacillus peoriae JC-3jx. This bacteria exhibited varying degrees of inhibition against the six pathogenic plant fungi tested, achieving a significant 56% inhibition rate against Pyricularia oryzae. Furthermore, the sterile fermentation supernatant of JC-3jx demonstrated pronounced inhibitory activity against the three tested pathogenic bacteria, particularly the gram-negative Escherichia coli. Moreover, the results demonstrate that the bacterial suspension of JC-3jx significantly promoted the rooting of corn seeds and the growth of Dendrobium nobile plantlets, indicating its excellent antibacterial and growth-promoting potential.

Cell enrichment is a powerful tool in many kinds of cell research, especially in applications with low abundance cell types. In this work, we developed a microfluidic fluorescence activated cell sorting (μFACS) device that is able to perform on-demand, low loss cell detection and sorting. The chip utilizes three-dimensional acoustic standing waves to position all cells in the same fluid velocity regime without sheath. When the cells pass through a laser interrogation region, the scattering and fluorescent signals are detected, translated and transported to software. The target cells are then identified by gating on the plots. Short bursts of standing acoustic waves are triggered by order from PC to sort target cells within predefined gating region. For very low abundance and rare labeled lymphocyte mixed with high concentration unlabeled white blood cells (WBCs), (1-100 labeled lymphocytes are diluted in 106 WBCs in 1 mL volume fluid), the device is able to remove more than 98% WBCs and recover labeled lymphocyte with efficiency of 80%. We further demonstrated that this device worked with real clinical samples by successfully isolating fetal nucleated red blood cells (FNRBCs) in the blood samples from pregnant women with male fetus. The obtained cells were sequenced and the expressions of (sex determining region Y) SRY genes were tested to determine fetal cell proportion. In genetic analysis, the proportion of fetal cells in the final picked sample is up to 40.64%. With this ability, the device proposed could be valuable for biomedical applications involving fetal cells, circulating tumor cells, and stem cells. This article is protected by copyright. All rights reserved.

Stropharia rugosoannulata (S. rugosoannulata) is a fungus with great edible and nutritional values; however, the development mechanism of its fruiting body has not been studied. Thus, this study aimed to analyze the differentially expressed genes (DEGs) in four stages; primordia stage (Sra1), young mushroom stage (Sra2), picking stage (Sra3), and opening umbrella stage (Sra4). Therefore, total RNA was extracted for further RNA-sequencing analysis. In three pairwise comparison groups (PCGs), Sra1 vs. Sra2, Sra2 vs. Sra3, and Sra3 vs. Sra4, a total of 3,112 DEGs were identified among the three PCGs. A GO analysis of the DEGs showed that there were 21 terms significantly enriched in Sra1 vs. Sra2 PCG. There was no significantly enriched GO term in the other two PCGs. Furthermore, KEGG pathway analysis showed that these DEGs were mainly enriched in glucose and amino acid metabolisms. Moreover we found that intron retention (IR) and the alternative 3′ splice site (A3SS) accounted for more than 80%. The development of the S. rugosoannulata fruiting body mainly involved glucose and amino acid metabolisms. IR and A3SS were the two main types of ASE, which played an important role in the development and maturation of the S. rugosoannulata fruiting body.

In this paper, the complete accident analysis calculation model of the ship nuclear power platform is established by using the developed accident analysis system code SACO (System Analysis Code under Ocean conditons), which is coupled with the ocean condition. Combined with the accident progress of loss of main feedwater ATWS, and on the basis of considering the reasonable assumption of the accident boundary, the main feedwater loss ATWS accident is analyzed. The accident sequence of loss of main feedwater ATWS is given, and the changes of the key thermal safety parameters such as the reactor power, heat removal from the active residual heat removal system, primary circuit pressure, pressurizer water volume, average reactor coolant temperature, peak temperatures of the fuel pellet and cladding are analyzed in detail. The results show the active residual heat removal system, reactor core reactivity feedback and design of the pressurizer safety valve could limit the primary circuit pressure and other key parameters without exceeding the safety limit value in the case of loss of main feedwater ATWS accident for ship nuclear power platform.

In cancer therapy, chemotherapeutic drugs frequently encounter multidrug resistance (MDR) induced by the overexpression of drug transporters such as P-glycoprotein (P-gp). Herein, in order to overcome MDR and improve the effectiveness of chemotherapy, we developed a novel pH-sensitive charge-reversal and NO generation liposomal system by modifying a pH-sensitive polymer (PEG-PLL-DMA) on the surface of cationic liposomes for delivering a NO donor (DETA NONOate) and a chemotherapy drug (paclitaxel, PTX) into MDR cells. The proposed liposomal system (PTX/NO/DMA-L) exhibited a distinctive charge-reversal capacity, which was negatively charged under physiological conditions (pH 7.4) but could reverse to positive charge in a tumor microenvironment (pH 6.5) due to the cleavable amide linkages formed between PEG-PLL and DMA, leading to the improvement of cell uptake. Once arrived in the endosomes and lysosomes (pH 5.0), DETA NONOate was triggered to decompose and release NO, which further promoted the quick release of PTX and inhibited the P-gp mediated efflux. The charge-reversal, NO generation and NO-triggered rapid release of drugs could significantly increase the accumulation of PTX in tumors and eventually improve the antitumor efficacy. These results indicate that this dual pH-sensitive liposomal system is a highly promising approach for chemotherapy and may pave a new avenue for overcoming MDR in cancer.