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Processing bodies (p-bodies) are a prototypical phase-separated RNA-containing granule. Their abundance is highly dynamic and has been linked to translation. Yet, the molecular mechanisms responsible for coordinate control of the two processes are unclear. Here, we uncover key roles for eEF2 kinase (eEF2K) in the control of ribosome availability and p-body abundance. eEF2K acts on a sole known substrate, eEF2, to inhibit translation. We find that the eEF2K agonist nelfinavir abolishes p-bodies in sensory neurons and impairs translation. To probe the latter, we used cryo-electron microscopy. Nelfinavir stabilizes vacant 80S ribosomes. They contain SERBP1 in place of mRNA and eEF2 in the acceptor site. Phosphorylated eEF2 associates with inactive ribosomes that resist splitting in vitro. Collectively, the data suggest that eEF2K defines a population of inactive ribosomes resistant to recycling and protected from degradation. Thus, eEF2K activity is central to both p-body abundance and ribosome availability in sensory neurons.

The meanings of words change over time. This is the central notion in Raymond Williams' 1976 book Keywords: A Vocabulary of Culture and Society and subsequent works (Bennett, Grossberg, & [...]

Contemporary concerns for college programs focus on the ability of the program to prepare students to become employable upon graduation. For a community college psychology program, that focus is somewhat muted by the fact that many of the community college graduates will transfer to four-year state universities. For this reason, it is imperative for the community college programs to ascertain from those four-year institutions what preparation those transferring students need. This study has captured what fourteen state public university psychology departments desire for transferring students and what they see as lacking in their students transferring from community colleges. The responding department chairs strongly cited basic scholarship skills, a stronger orientation toward critical and scientific thinking, and a professional and career focus in the transferring students.