Search

Your search keyword '"Smet, Annemieke"' showing total 419 results
Start Over Author "Smet, Annemieke"
419 results on '"Smet, Annemieke"'

102. Helicobacter suis binding to carbohydrates on human and porcine gastric mucins and glycolipids occurs via two modes

Author

Padra, Médea, primary, Adamczyk, Barbara, additional, Benktander, John, additional, Flahou, Bram, additional, Skoog, Emma C., additional, Padra, János Tamás, additional, Smet, Annemieke, additional, Jin, Chunsheng, additional, Ducatelle, Richard, additional, Samuelsson, Tore, additional, Haesebrouck, Freddy, additional, Karlsson, Niclas G., additional, Teneberg, Susann, additional, and Lindén, Sara K., additional

Published
2018
Full Text
View/download PDF

103. Sa1172 - Effects of the Non-Selective Protease Inhibitor Nafamostat Mesylate on Intestinal Permeability and Bacterial Translocation in a Murine Model of Sepsis

Author

Plaeke, Philip, primary, De Man, Joris, additional, Hens, Sebastiaan, additional, Dua, Cedric, additional, Nullens, Sara, additional, Smet, Annemieke, additional, Malhotra-Kumar, Surbhi, additional, Jorens, Philippe, additional, Hubens, Guy, additional, and De Winter, Benedicte Y., additional

Published
2018
Full Text
View/download PDF

104. A Potential New Human Pathogen Belonging to Helicobacter Genus, Identified in a Bloodstream Infection

Author

van der Mee-Marquet, Nathalie L., primary, Bénéjat, Lucie, additional, Diene, Seydina M., additional, Lemaignen, Adrien, additional, Gaïa, Nadia, additional, Smet, Annemieke, additional, Haesebrouck, Freddy, additional, Cherkaoui, Abdessalam, additional, Ducournau, Astrid, additional, Lacomme, Sabrina, additional, Gontier, Etienne, additional, Bernard, Louis, additional, Mégraud, Francis, additional, Goudeau, Alain, additional, Lehours, Philippe, additional, and François, Patrice, additional

Published
2017
Full Text
View/download PDF

105. United European Gastroenterology (UEG) and European Society for Neurogastroenterology and Motility (ESNM) consensus on functional dyspepsia

Author

Wauters, Lucas, Dickman, Ram, Drug, Vasile, Mulak, Agata, Serra, Jordi, Enck, Paul, Tack, Jan, Accarino, Anna, Barbara, Giovanni, Bor, Serhat, Coffin, Benoit, Corsetti, Maura, De Schepper, Heiko, Dumitrascu, Dan, Farmer, Adam, Gourcerol, Guillaume, Hauser, Goran, Hausken, Trygve, Karamanolis, George, Keszthelyi, Daniel, Malagelada, Carolin, Milosavljevic, Tomislav, Muris, Jean, O’Morain, Colm, Papathanasopoulos, Athanassos, Pohl, Daniel, Rumyantseva, Diana, Sarnelli, Giovanni, Savarino, Edoardo, Schol, Jolien, Sheptulin, Arkady, Smet, Annemieke, Stengel, Andreas, Storonova, Olga, Storr, Martin, Törnblom, Hans, Vanuytsel, Tim, Velosa, Monica, Waluga, Marek, Zarate, Natalia, and Zerbib, Frank

Abstract

Functional dyspepsia (FD) is one of the most common conditions in clinical practice. In spite of its prevalence, FD is associated with major uncertainties in terms of its definition, underlying pathophysiology, diagnosis, treatment, and prognosis. A Delphi consensus was initiated with 41 experts from 22 European countries who conducted a literature summary and voting process on 87 statements. Quality of evidence was evaluated using the grading of recommendations, assessment, development, and evaluation (GRADE) criteria. Consensus (defined as >80% agreement) was reached for 36 statements. The panel agreed with the definition in terms of its cardinal symptoms (early satiation, postprandial fullness, epigastric pain, and epigastric burning), its subdivision into epigastric pain syndrome and postprandial distress syndrome, and the presence of accessory symptoms (upper abdominal bloating, nausea, belching), and overlapping conditions. Also, well accepted are the female predominance of FD, its impact on quality of life and health costs, and acute gastrointestinal infections, and anxiety as risk factors. In terms of pathophysiological mechanisms, the consensus supports a role for impaired gastric accommodation, delayed gastric emptying, hypersensitivity to gastric distention, Helicobacter pyloriinfection, and altered central processing of signals from the gastroduodenal region. There is consensus that endoscopy is mandatory for establishing a firm diagnosis of FD, but that in primary care, patients without alarm symptoms or risk factors can be managed without endoscopy. There is consensus that H. pyloristatus should be determined in every patient with dyspeptic symptoms and H. pyloripositive patients should receive eradication therapy. Also, proton pump inhibitor therapy is considered an effective therapy for FD, but no other treatment approach reached a consensus. The long‐term prognosis and life expectancy are favorable. A multinational group of European experts summarized the current state of consensus on the definition, diagnosis and management of FD. Current knowledge Functional dyspepsia is one of the most common conditions encountered in clinical practice.There is a lack of guidance for clinicians in guiding diagnosis and treatment of this prevalent condition.No treatments are currently approved for the treatment of functional dyspepsia in Europe. Functional dyspepsia is one of the most common conditions encountered in clinical practice. There is a lack of guidance for clinicians in guiding diagnosis and treatment of this prevalent condition. No treatments are currently approved for the treatment of functional dyspepsia in Europe. What is new here A Delphi panel consisting of 41 experts from 22 European countries established the level of consensus on 87 statements regarding functional dyspepsia.The statements reaching consensus serve to guide clinicians in recognizing, diagnosing and treating FD in clinical practice.Endoscopy is mandatory for establishing a firm diagnosis of functional dyspepsia D, but in primary care patients without alarm symptoms or risk factors can be managed without endoscopy.Helicobacter pyloristatus should be determined in every patient with dyspeptic symptoms and H. Pyloripositive patients should receive eradication therapy.Proton pump inhibitor‐therapy is considered an effective therapy for FD, but no other treatment approach reached consensus support. A Delphi panel consisting of 41 experts from 22 European countries established the level of consensus on 87 statements regarding functional dyspepsia. The statements reaching consensus serve to guide clinicians in recognizing, diagnosing and treating FD in clinical practice. Endoscopy is mandatory for establishing a firm diagnosis of functional dyspepsia D, but in primary care patients without alarm symptoms or risk factors can be managed without endoscopy. Helicobacter pyloristatus should be determined in every patient with dyspeptic symptoms and H. Pyloripositive patients should receive eradication therapy. Proton pump inhibitor‐therapy is considered an effective therapy for FD, but no other treatment approach reached consensus support.

Published
2021
Full Text
View/download PDF

106. The Helicobacter heilmannii hofE and hofF Genes are Essential for Colonization of the Gastric Mucosa and Play a Role in IL-1β-Induced Gastric MUC13 Expression

Author

Rossi Mirko, Haesebrouck Freddy, Van den Broeck Wim, Blaecher Caroline, Ducatelle Richard, Flahou Bram, Lindén Sara, Padra Medea, Bauwens Eva, Joosten Myrthe, Liu Cheng, and Smet Annemieke

Subjects
0301 basic medicine, 030106 microbiology, Mutant, Helicobacter heilmannii, Interleukin-1beta, Biology, Bacterial Adhesion, Microbiology, 03 medical and health sciences, medicine, Gastric mucosa, Animals, Helicobacter, Adhesins, Bacterial, Mice, Inbred BALB C, Epidermal Growth Factor, Stomach, digestive, oral, and skin physiology, Mucin, Gastroenterology, Wild type, General Medicine, biology.organism_classification, digestive system diseases, Bacterial adhesin, 030104 developmental biology, Infectious Diseases, medicine.anatomical_structure, Gastric Mucosa, Antigens, Surface, Host-Pathogen Interactions, Female
Abstract

Background Helicobacter heilmannii is a zoonotic bacterium associated with gastric disease in humans. We recently showed that H. heilmannii binds to human gastric mucins and epithelial cells and highlighted a potential role for the murine Muc13 mucin in gastric Helicobacter colonization. The aims of this study were to investigate the role of the H. heilmannii hof gene locus encoding HofH/F/E/G/C/D in adhesion to the gastric mucosa and induction of increased gastric Muc13 expression. Methods Bacterial hof gene and host gene expression experiments, Helicobacter binding assays and experimental infection studies in mice were performed. H. pylori and its ΔhofF mutant were included for comparison. Results Helicobacter heilmannii strains lacking HofE or HofF showed a clear decrease in binding to gastric mucins and epithelial cells as well as a lower gastric colonization level in the stomach of Balb/c mice at 4 and 9 weeks post-infection compared to the H. heilmannii wildtype strain. Interestingly, H. heilmannii ΔhofE and ΔhofF and H. pylori ΔhofF did not induce an increased expression of MUC13 in human gastric epithelial cells and of Muc13 in the stomach of mice. Finally, we demonstrated that IL-1β is induced in the stomach as a response to Helicobacter colonization which on its turn is involved in the expression of MUC13/Muc13 in the gastric epithelium. Conclusion These novel results in Helicobacter research identified H. heilmannii HofE and HofF as adhesins and suggest an important role of H. heilmannii HofE and HofF and H. pylori HofF in IL-1β-induced gastric MUC13/Muc13 expression.

Published
2016

107. Additional file 1: Table S1. of Residues of chlortetracycline, doxycycline and sulfadiazine-trimethoprim in intestinal content and feces of pigs due to cross-contamination of feed

Author

Peeters, Laura, Daeseleire, Els, Devreese, Mathias, Geertrui Rasschaert, Smet, Annemieke, Dewulf, Jeroen, Heyndrickx, Marc, Imberechts, Hein, Haesebrouck, Freddy, Butaye, Patrick, and Croubels, Siska

Abstract

Validation parameters for quantification of chlortetracycline (CTC), doxycycline (DOX), sulfadiazine (SDZ) and trimethoprim (TRIM) in pig feed and feces. (DOCX 15 kb)

Published
2016
Full Text
View/download PDF

108. Evidence for a primate origin of zoonotic Helicobacter suis colonizing domesticated pigs

Author

Flahou, Bram, primary, Rossi, Mirko, additional, Bakker, Jaco, additional, Langermans, Jan A M, additional, Heuvelman, Edwin, additional, Solnick, Jay V, additional, Martin, Miriam E, additional, O’Rourke, Jani, additional, Ngoan, Le Duc, additional, Hoa, Nguyen Xuan, additional, Nakamura, Masahiko, additional, Øverby, Anders, additional, Matsui, Hidenori, additional, Ota, Hiroyoshi, additional, Matsumoto, Takehisa, additional, Foss, Dennis L, additional, Kopta, Laurice A, additional, Omotosho, Oladipo, additional, Franciosini, Maria Pia, additional, Casagrande Proietti, Patrizia, additional, Guo, Aizhen, additional, Liu, Han, additional, Borilova, Gabriela, additional, Bracarense, Ana Paula, additional, Lindén, Sara K, additional, De Bruyckere, Sofie, additional, Zhang, Guangzhi, additional, De Witte, Chloë, additional, Smet, Annemieke, additional, Pasmans, Frank, additional, Ducatelle, Richard, additional, Corander, Jukka, additional, and Haesebrouck, Freddy, additional

Published
2017
Full Text
View/download PDF

110. Species-specific immunity to Helicobacter suis

Author

Bosschem, Iris, primary, Flahou, Bram, additional, Van Deun, Kim, additional, De Koker, Stefaan, additional, Volf, Jiri, additional, Smet, Annemieke, additional, Ducatelle, Richard, additional, Devriendt, Bert, additional, and Haesebrouck, Freddy, additional

Published
2017
Full Text
View/download PDF

113. Residues of chlortetracycline, doxycycline and sulfadiazine-trimethoprim in intestinal content and feces of pigs due to cross-contamination of feed

Author

Peeters, Laura E. J., primary, Daeseleire, Els, additional, Devreese, Mathias, additional, Rasschaert, Geertrui, additional, Smet, Annemieke, additional, Dewulf, Jeroen, additional, Heyndrickx, Marc, additional, Imberechts, Hein, additional, Haesebrouck, Freddy, additional, Butaye, Patrick, additional, and Croubels, Siska, additional

Published
2016
Full Text
View/download PDF

118. Divergence between the Highly Virulent Zoonotic Pathogen Helicobacter heilmannii and Its Closest Relative, the Low-Virulence “Helicobacter ailurogastricus” sp. nov

Author

Joosten, Myrthe, primary, Lindén, Sara, additional, Rossi, Mirko, additional, Tay, Alfred Chin Yen, additional, Skoog, Emma, additional, Padra, Médea, additional, Peters, Fanny, additional, Perkins, Tim, additional, Vandamme, Peter, additional, Van Nieuwerburgh, Filip, additional, D'Herde, Katharina, additional, Van den Broeck, Wim, additional, Flahou, Bram, additional, Deforce, Dieter, additional, Ducatelle, Richard, additional, Marshall, Barry, additional, Haesebrouck, Freddy, additional, and Smet, Annemieke, additional

Published
2016
Full Text
View/download PDF

119. Helicobacter suisbinding to carbohydrates on human and porcine gastric mucins and glycolipids occurs via two modes

Author

Padra, Médea, Adamczyk, Barbara, Benktander, John, Flahou, Bram, Skoog, Emma C., Padra, János Tamás, Smet, Annemieke, Jin, Chunsheng, Ducatelle, Richard, Samuelsson, Tore, Haesebrouck, Freddy, Karlsson, Niclas G., Teneberg, Susann, and Lindén, Sara K.

Abstract

ABSTRACTHelicobacter suiscolonizes the stomach of most pigs and is the most prevalent non-Helicobacter pyloriHelicobacter species found in the human stomach. In the human host, H. suiscontributes to the development of chronic gastritis, peptic ulcer disease and MALT lymphoma, whereas in pigs it is associated with gastritis, decreased growth and ulcers. Here, we demonstrate that the level of H. pyloriand H. suisbinding to human and pig gastric mucins varies between individuals with species dependent specificity. The binding optimum of H. pyloriis at neutral pH whereas that of H. suishas an acidic pH optimum, and the mucins that H. pyloribind to are different than those that H. suisbind to. Mass spectrometric analysis of mucin O-glycans from the porcine mucin showed that individual variation in binding is reflected by a difference in glycosylation; of 109 oligosaccharide structures identified, only 14 were present in all examined samples. H. suisbinding to mucins correlated with glycans containing sulfate, sialic acid and terminal galactose. Among the glycolipids present in pig stomach, binding to lactotetraosylceramide (Galβ3GlcNAcβ3Galβ4Glcβ1Cer) was identified, and adhesion to Galβ3GlcNAcβ3Galβ4Glc at both acidic and neutral pH was confirmed using other glycoconjugates. Together with that H. suisbound to DNA (used as a proxy for acidic charge), we conclude that H. suishas two binding modes: one to glycans terminating with Galβ3GlcNAc, and one to negatively charged structures. Identification of the glycan structures H. suisinteracts with can contribute to development of therapeutic strategies alternative to antibiotics.

Published
2018
Full Text
View/download PDF

120. In situ ESBL conjugation from avian to human Escherichia coli during cefotaxime administration

Author

Smet, Annemieke, Rasschaert, Geertrui, Martel, An, Persoons, Davy, Dewulf, Jeroen, Butaye, Patrick, Catry, Boudewijn, Haesebrouck, Freddy, Herman, Lieve, and Heyndrickx, Marc

Subjects
extended-spectrum beta-lactams, ANTIMICROBIAL RESISTANCE, VIVO TRANSFER, DIGESTIVE-TRACT, Biology and Life Sciences, ANTIBIOTIC-RESISTANCE, SALMONELLA, continuous flow model, GENE-TRANSFER, FARM-ANIMALS, SPECTRUM BETA-LACTAMASES, KLEBSIELLA-PNEUMONIAE, transfer frequency, conjugation, AMPICILLIN RESISTANCE
Abstract

Aims: The behaviour of an E. coli isolate of broiler origin harbouring a blaTEM-52-carrying plasmid (lactose negative mutant of B1-54, IncII group) was studied in an in situ continuous flow culture system, simulating the human caecum and the ascending colon during cefotaxime administration. Methods and Results: Fresh faeces from a healthy volunteer, negative for cephalosporin-resistant E. coli, were selected to prepare inocula. The microbiota was monitored by plating on diverse selective media, and a shift in the populations of bacteria was examined by 16S rDNA PCR denaturing gradient gel electrophoresis. E. coli transconjugants were verified by plasmid and pulsed field gel electrophoresis profiles (PFGE). The avian extended-spectrum β-lactamase (ESBL) positive E. coli was able to proliferate without selective pressure of cefotaxime and E. coli transconjugants of human origin were detected 24 h after inoculation of the donor strain. Upon administration of cefotaxime to the fresh medium, an increase of the population size of E. coli B1-54 and the transconjugants was observed. PFGE and plasmid analysis revealed a limited number of human E. coli clones receptive for the blaTEM-52-carrying plasmid. Conclusions: These observations provide evidence of the maintenance of an E. coli strain of poultry origin and the horizontal gene transfer in the human commensal bowel microbiota even without antimicrobial treatment. Significance and Impact of Study: The fact that an E. coli strain of poultry origin might establish itself and transfer its bla gene to commensal human E. coli raises public health concerns.

Published
2011

124. Presence of helicobacter suis in the oral secretions of slaughtered pigs

Author

Smet, Annemieke, Pasmans, Frank, Van Deun, Kim, Flahou, Bram, Ducatelle, Richard, and Haesebrouck, Freddy

Subjects
Veterinary Sciences
Abstract

The main Helicobacter species colonizing the stomach of pigs is H. suis which causes gastritis in experimentally and naturally infected pigs. This bacterium has also been associated with ulcers of the nonglandular part of the porcine stomach and is of zoonotic significance. The definite mode of transmission of H. suis between pigs and from pigs to humans remains to be investigated. This study was carried out to determine if the oral secretions of pigs might be a possible route of transmission. Oral swabs were taken from slaughtered pigs originating from 4 different herds (10 pigs/herd). DNA was extracted from these swabs with PrepMan sample preparation reagent from Applied Biosystems as described by the manufacturer. The presence of H. suis DNA was examined using a quantitative PCR based on the urease A gene. In each herd, 2-3 of the 10 swabs were positive for H. suis DNA. These positive samples contained 3000 to 60 000 bacteria. Further studies are required to determine viability of these bacteria.

Published
2010

125. Isolation and characterization of a new Helicobacter sp. belonging to the 'Helicobacter heilmannii' type 2 group from feline stomachs

Author

Smet, Annemieke, Pasmans, Frank, Flahou, Bram, D'Herde, Katharina, Vandamme, Peter, Cleenwerck, Ilse, Ducatelle, Richard, and Haesebrouck, Freddy

Subjects
Veterinary Sciences
Abstract

Three Gram-negative, microaerophillic bacteria with a corkscrew-like morphology isolated from the gastric mucosa of cats and designated ASB1, ABS2 and ASB3, were subjected to a polyphasic taxonomic study. The isolates grew on biphasic culture plates in microaerobic conditions at 37°C and exhibited urease, oxidase and catalase activity. They were also able to grow in colonies on dry agar plates. Based on 16S rRNA gene sequence analysis, ASB1, ABS2 and ASB3 were identified as a member of the genus Helicobacter and showed 98 to 99% sequence similarity to H. felis, H. bizzozeronii, Candidatus H. heilmannii, H. cynogastricus and H. salomonis, five related Helicobacter species belonging to the “Helicobacter heilmannii” type 2 group and previously detected in the feline or canine gastric mucosa. Sequencing of the partial hsp60 gene demostrated that ASB1, ASB2 and ASB3 constitute a separate taxon in the “Helicobacter heilmannii” type 2 group. The urease gene sequences of ASB1, ASB2 and ASB3 showed approximately 92% homology to the urease gene sequences of “Candidatus Helicobacter heilmannii” described by O’Rourke et al. (2004). Protein profiling of the strains ASB1, ASB2 and ASB3 using SDS-PAGE also allowed to differentiate them from other Helicobacter species of feline or canine gastric origin. The results of this polyphasic taxonomic study indicate that the cultured isolates constitute a new taxon corresponding to “Candidatus Helicobacter heilmannii” from cats but further investigations are still needed.

Published
2010

130. A novel isolation protocol and probe-based RT- PCR for diagnosis of gastric infections with the zoonotic pathogen Helicobacter suis.

Author

Blaecher, Caroline, Bauwens, Eva, Tay, Alfred, Peters, Fanny, Dobbs, Sylvia, Dobbs, John, Charlett, André, Ducatelle, Richard, Haesebrouck, Freddy, and Smet, Annemieke

Subjects
HELICOBACTER pylori infections, GASTRIC disease diagnosis, ISOLATION (Hospital care), ZOONOSES, HEALTH outcome assessment, DIAGNOSIS
Abstract

Background Helicobacter suis is a very fastidious microorganism associated with gastritis, gastric ulcers, and mucosa-associated lymphoid tissue lymphoma in humans. In vitro isolation of this agent from human patients has so far been unsuccessful. Materials and methods A probe-based real-time PCR ( RT- PCR) for the rapid detection of H. suis in gastric biopsies was developed. Secondly, a mouse-passage-based protocol was optimized for isolation of low numbers of viable H. suis bacteria. Mice were inoculated with different numbers of viable H. suis (102-108) and kept for 4 weeks to allow multiplication of this pathogen. Results The probe-based real-time PCR ( RT- PCR) exhibited a high degree of diagnostic specificity and analytical sensitivity, high linear correlations ( r2 between 0.995 and 0.999), and high amplification efficiencies (>90%) for H. suis. No cross-reactivity was detected with human, porcine, non-human primate, and murine DNA nor with DNA from other bacteria including Helicobacter spp. and Campylobacter spp. H. suis was successfully re-isolated from the stomach of mice inoculated with at least 104 viable H. suis, using a biphasic medium ( pH 5), consisting of Brucella agar with Brucella broth on top, both supplemented with vitox supplement, Campylobacter-selective supplement, amphotericin (5 μg/mL), HCl (0.05%), fetal bovine serum (20%), and linezolid (5 μg/mL). Linezolid was necessary to inhibit proliferation of contaminants, including lactobacilli. Conclusion The methods described above can be implemented for detection or isolation of H. suis from human gastric biopsies. [ABSTRACT FROM AUTHOR]

Published
2017
Full Text
View/download PDF

131. Comparative virulence of in vitro-cultured primate- and pig-associated Helicobacter suis strains in a BALB/c mouse and a Mongolian gerbil model.

Author

Bosschem, Iris, Flahou, Bram, Bakker, Jaco, Heuvelman, Edwin, Langermans, Jan A.M., De Bruyne, Ellen, Joosten, Myrthe, Smet, Annemieke, Ducatelle, Richard, and Haesebrouck, Freddy

Subjects
HELICOBACTER diseases, MUCOSA-associated lymphoid tissue lymphoma, MACAQUES, MICROBIAL virulence, SWINE, MONGOLIAN gerbil, MICE, KRA
Abstract

Background Helicobacter suis ( H. suis) is the most prevalent gastric non- H. pylori Helicobacter species in humans. This bacterium mainly colonizes the stomach of pigs, but it has also been detected in the stomach of nonhuman primates. The aim of this study was to obtain better insights into potential differences between pig- and primate-associated H. suis strains in virulence and pathogenesis. Materials and Methods In vitro-isolated H. suis strains obtained from pigs, cynomolgus monkeys ( Macaca fascicularis), and rhesus monkeys ( Macaca mulatta) were used for intragastric inoculation of BALB/c mice and Mongolian gerbils. Nine weeks and six months later, samples of the stomach of inoculated and control animals were taken for PCR analysis and histopathological examination. Results The cynomolgus monkey-associated H. suis strain only colonized the stomach of mice, but not of Mongolian gerbils. All other H. suis strains colonized the stomach in both rodent models. In all colonized animals, severe gastric inflammation was induced. Gastric lymphoid follicles and destruction of the antral epithelium were observed in infected gerbils, but not in mice. Infection with both pig- and primate-associated H. suis strains evoked a similar marked Th17 response in mice and gerbils, accompanied by increased CXCL-13 expression levels. Conclusions Apart from the cynomolgus monkey-associated strain which was unable of colonizing the stomach of Mongolian gerbils, no substantial differences in virulence were found in rodent models between in vitro-cultured pig-associated, cynomolgus monkey-associated and rhesus monkey-associated H. suis strains. The experimental host determines the outcome of the immune response against H. suis infection, rather than the original host. [ABSTRACT FROM AUTHOR]

Published
2017
Full Text
View/download PDF

134. A comparison ofHelicobacter pyloriand non-Helicobacter pylori Helicobacterspp. Binding to Canine Gastric Mucosa with Defined Gastric Glycophenotype

Author

Amorim, Irina, primary, Freitas, Daniela P., additional, Magalhães, Ana, additional, Faria, Fátima, additional, Lopes, Célia, additional, Faustino, Augusto M., additional, Smet, Annemieke, additional, Haesebrouck, Freddy, additional, Reis, Celso A., additional, and Gärtner, Fátima, additional

Published
2014
Full Text
View/download PDF

135. Correction: Effects of Helicobacter suis γ- Glutamyl Transpeptidase on Lymphocytes: Modulation by Glutamine and Glutathione Supplementation and Outer Membrane Vesicles as a Putative Delivery Route of the Enzyme

Author

Zhang, Guangzhi, primary, Ducatelle, Richard, additional, Pasmans, Frank, additional, D’Herde, Katharina, additional, Huang, Liping, additional, Smet, Annemieke, additional, Haesebrouck, Freddy, additional, and Flahou, Bram, additional

Published
2014
Full Text
View/download PDF

136. Macroevolution of gastric Helicobacterspecies unveils interspecies admixture and time of divergence

Author

Smet, Annemieke, Yahara, Koji, Rossi, Mirko, Tay, Alfred, Backert, Steffen, Armin, Ensser, Fox, James, Flahou, Bram, Ducatelle, Richard, Haesebrouck, Freddy, and Corander, Jukka

Abstract

Since the discovery of the human pathogen Helicobacter pylori, various other Helicobacterspecies have been identified in the stomach of domesticated and wild mammals. To better understand the evolutionary history of these ecologically similar but genetically distinct species, we analyzed 108 gastric Helicobactergenomes and included 54 enterohepatic Helicobactergenomes for comparison purposes. An admixture analysis supported the presence of an ecological barrier, preventing the genetic exchange between the gastric and enterohepatic Helicobacterspecies, and unraveled many gene flow events within and across species residing in the stomach. As pets can be colonized by multiple gastric Helicobacterspecies, the genetic exchange between the canine and feline strains was evident, with H. heilmanniiand H. bizzozeroniishowing the highest interspecies recombination. An admixture between H. pylori(in particular, the ancestral African strains), H. acinonychisfrom wild felines and H. cetorumfrom marine mammals was also identified. Because these latter species do not share the same host, this phenomenon is most likely a remaining signal of shared ancestry. A reconstruction of the time of divergence of the gastric Helicobacterspp. revealed that the domestic animal-related Helicobacterspecies evolved in parallel with H. pyloriand its two closest relatives (H. acinonychisand H. cetorum), rather than together.

Published
2018
Full Text
View/download PDF

137. Evidence for a primate origin of zoonotic Helicobacter suiscolonizing domesticated pigs

Author

Flahou, Bram, Rossi, Mirko, Bakker, Jaco, Langermans, Jan A M, Heuvelman, Edwin, Solnick, Jay V, Martin, Miriam E, O’Rourke, Jani, Ngoan, Le Duc, Hoa, Nguyen Xuan, Nakamura, Masahiko, Øverby, Anders, Matsui, Hidenori, Ota, Hiroyoshi, Matsumoto, Takehisa, Foss, Dennis L, Kopta, Laurice A, Omotosho, Oladipo, Franciosini, Maria Pia, Casagrande Proietti, Patrizia, Guo, Aizhen, Liu, Han, Borilova, Gabriela, Bracarense, Ana Paula, Lindén, Sara K, De Bruyckere, Sofie, Zhang, Guangzhi, De Witte, Chloë, Smet, Annemieke, Pasmans, Frank, Ducatelle, Richard, Corander, Jukka, and Haesebrouck, Freddy

Abstract

Helicobacter suisis the second most prevalent Helicobacterspecies in the stomach of humans suffering from gastric disease. This bacterium mainly inhabits the stomach of domesticated pigs, in which it causes gastric disease, but it appears to be absent in wild boars. Interestingly, it also colonizes the stomach of asymptomatic rhesus and cynomolgus monkeys. The origin of modern human-, pig- or non-human primate-associated H. suisstrains in these respective host populations was hitherto unknown. Here we show that H. suisin pigs possibly originates from non-human primates. Our data suggest that a host jump from macaques to pigs happened between 100?000 and 15?000 years ago and that pig domestication has had a significant impact on the spread of H. suisin the pig population, from where this pathogen occasionally infects humans. Thus, in contrast to our expectations, H. suisappears to have evolved in its main host in a completely different way than its close relative Helicobacter pyloriin humans.

Published
2018
Full Text
View/download PDF

144. Helicobacter suis affects the health and function of porcine gastric parietal cells.

Author

Guangzhi Zhang, Ducatelle, Richard, Mihi, Belgacem, Smet, Annemieke, Flahou, Bram, and Haesebrouck, Freddy

Abstract

The stomach of pigs at slaughter age is often colonized by Helicobacter (H.) suis, which is also the most prevalent gastric non-H. pylori Helicobacter (NHPH) species in humans. It is associated with chronic gastritis, gastric ulceration and other gastric pathological changes in both hosts. Parietal cells are highly specialized, terminally differentiated epithelial cells responsible for gastric acid secretion and regulation. Dysfunction of these cells is closely associated with gastric pathology and disease. Here we describe a method for isolation and culture of viable and responsive parietal cells from slaughterhouse pigs. In addition, we investigated the interactions between H. suis and gastric parietal cells both in H. suis-infected six-month-old slaughter pigs, as well as in our in vitro parietal cell model. A close interaction of H. suis and parietal cells was observed in the fundic region of stomachs from H. suis positive pigs. The bacterium was shown to be able to directly interfere with cultured porcine parietal cells, causing a significant impairment of cell viability. Transcriptional levels of Atp4a, essential for gastric acid secretion, showed a trend towards an up-regulation in H. suis positive pigs compared to H. suis-negative pigs. In addition, sonic hedgehog, an important factor involved in gastric epithelial differentiation, gastric mucosal repair, and stomach homeostasis, was also significantly up-regulated in H. suis positive pigs. In conclusion, this study describes a successful approach for the isolation and culture of porcine gastric parietal cells. The results indicate that H. suis affects the viability and function of this cell type. [ABSTRACT FROM AUTHOR]

Published
2016
Full Text
View/download PDF

145. Residues of chlortetracycline, doxycycline and sulfadiazine-trimethoprim in intestinal content and feces of pigs due to crosscontamination of feed.

Author

Peeters, Laura E. J., Daeseleire, Els, Devreese, Mathias, Rasschaert, Geertrui, Smet, Annemieke, Dewulf, Jeroen, Heyndrickx, Marc, Imberechts, Hein, Haesebrouck, Freddy, Butaye, Patrick, and Croubels, Siska

Subjects
DOXYCYCLINE, SULFADIAZINE, TRIMETHOPRIM, GASTROINTESTINAL contents, SWINE nutrition, ANIMAL droppings, BIOAVAILABILITY
Abstract

Background: Cross-contamination of feed with low concentrations of antimicrobials can occur at production, transport and/or farm level. Concerns are rising about possible effects of this contaminated feed on resistance selection in the intestinal microbiota. Therefore, an experiment with pigs was set up, in which intestinal and fecal concentrations of chlortetracycline (CTC), doxycycline (DOX) and sulfadiazine-trimethoprim (SDZ-TRIM) were determined after administration of feed containing a 3 % carry-over level of these antimicrobials. Results: The poor oral bioavailability of tetracyclines resulted in rather high concentrations in cecal and colonic content and feces at steady-state conditions. A mean concentration of 10 mg/kg CTC and 4 mg/kg DOX in the feces was reached, which is higher than concentrations that were shown to cause resistance selection. On the other hand, lower mean levels of SDZ (0.7 mg/kg) and TRIM (< limit of detection of 0.016 mg/kg) were found in the feces, corresponding with the high oral bioavailability of SDZ and TRIM in pigs. Conclusions: The relation between the oral bioavailability and intestinal concentrations of the tested antimicrobials, may be of help in assessing the risks of cross-contaminated feed. However, future research is needed to confirm our results and to evaluate the effects of these detected concentrations on resistance selection in the intestinal microbiota of pigs. [ABSTRACT FROM AUTHOR]

Published
2016
Full Text
View/download PDF

147. Gastric epithelial cell death caused by Helicobacter suis and Helicobacter pylori γ-glutamyl transpeptidase is mainly glutathione degradation-dependent

Author

Flahou, Bram, primary, Haesebrouck, Freddy, additional, Chiers, Koen, additional, Van Deun, Kim, additional, De Smet, Lina, additional, Devreese, Bart, additional, Vandenberghe, Isabel, additional, Favoreel, Herman, additional, Smet, Annemieke, additional, Pasmans, Frank, additional, D'Herde, Katharina, additional, and Ducatelle, Richard, additional

Published
2011
Full Text
View/download PDF

150. Characterization of Extended-Spectrum β-Lactamases Produced byEscherichia coliIsolated from Hospitalized and Nonhospitalized Patients: Emergence of CTX-M-15-Producing Strains Causing Urinary Tract Infections

Author

Smet, Annemieke, primary, Martel, An, additional, Persoons, Davy, additional, Dewulf, Jeroen, additional, Heyndrickx, Marc, additional, Claeys, Geert, additional, Lontie, Marc, additional, Van Meensel, Britt, additional, Herman, Lieve, additional, Haesebrouck, Freddy, additional, and Butaye, Patrick, additional

Published
2010
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results