138 results on '"Shimajiri S"'
Search Results
102. Rectal Carcinoma with Heterotopic Bone: Report of a Case.
- Author
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Nagao Y, Shimajiri S, Katsuki T, Nakayama Y, and Yamaguchi K
- Abstract
Heterotopic bone is rarely present in malignant tumors of the gastrointestinal tract. We herein report a case of rectal adenocarcinoma with heterotopic bone. A 46-year-old Japanese male presented to our hospital with abdominal distension and constipation. Colonoscopic examination showed an ulcerated polypoid tumor of the rectum which nearly obstructed the rectal lumen. Abdominal computed tomography showed a tumor of the rectum with calcified deposits. Low anterior resection with lateral lymph node dissection was performed under the tentative diagnosis of rectal cancer. Histological examination of the resected specimen showed mucinous carcinoma of the rectum with heterotopic bone. One of the metastatic lymph nodes dissected also showed heterotopic bone. In the present report, we describe this rare tumor and briefly review the pertinent literature regarding rectal cancer with heterotopic bone.
- Published
- 2010
- Full Text
- View/download PDF
103. [An autopsy case of microscopic polyangiitis associated with bacterial endocarditis].
- Author
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Wang KY, Shimajiri S, Yoshida T, Yamada S, and Sasaguri Y
- Subjects
- Aged, 80 and over, Antibodies, Antineutrophil Cytoplasmic blood, Autoantibodies blood, C-Reactive Protein analysis, Humans, Male, Endocarditis, Bacterial complications, Microscopic Polyangiitis pathology
- Abstract
The patient was an 87-year-old man whose initial symptom was general fatigue and inappetence. His laboratory data revealed a rise in C-reactive protein (CRP) and white blood cell count (WBC), and CT scan showed suspicious pneumonia. Antibiotics were given to the patient, but his fever and laboratory data were sustained. Follow up examination revealed a high titer (107 U/ml) of myeloperoxidase specific anti-neutrophil cytoplasmic autoantibody (MPO-ANCA). He was diagnosed with MPO-ANCA associated vasculitis. Steroid pulse therapy was started. The patient's clinical symptoms and laboratory data thereafter significantly improved, but after one week the patient's symptom was aggravated and he died. An autopsy was performed, and necrotizing arteritis of the interlobular arteries were found in the kidneys. We found bacterial infective vegetation attached to the aortic valve. Infected thromboembolus and microabscesses were also found in many organs. We report a case of subacute microscopic polyangiitis associated with bacterial endocarditis.
- Published
- 2010
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104. C5a promotes migration, proliferation, and vessel formation in endothelial cells.
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Kurihara R, Yamaoka K, Sawamukai N, Shimajiri S, Oshita K, Yukawa S, Tokunaga M, Iwata S, Saito K, Chiba K, and Tanaka Y
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- Animals, Cell Cycle drug effects, Cell Line, Complement Activation, Complement C5a antagonists & inhibitors, Endothelial Cells cytology, Endothelium, Vascular cytology, Endothelium, Vascular drug effects, Humans, Cell Movement drug effects, Cell Proliferation drug effects, Complement C5a immunology, Endothelial Cells drug effects, Endothelial Cells physiology, Neovascularization, Pathologic
- Abstract
Objectives: The goal of this paper is to investigate the effects of activated complement C5a on vascular endothelium during vessel formation., Methods: A human microvascular endothelial cell line (HMEC-1) derived from post-capillary venules in skin was used to measure DNA synthesis, proliferation and cell-cycle progression. In vitro ring-shaped formation by the cells was assessed by using type I collagen gel matrix and a cell-migration assay using the Chemotaxicell chamber. A Matrigel plug assay was performed to confirm the effect of C5a in vivo., Results: C5a progressed the cell cycle of HMEC-1 into G2/M phases, and induced DNA synthesis and proliferation in a dose-dependent manner. C5a efficiently induced migration and ring-shaped structure formation both in vitro and in vivo. Furthermore, a C5a receptor antagonist (W-54011) suppressed all HMEC-1 activities including proliferation and migration., Conclusions: Proliferation, migration, and ring-shaped formation by HMEC-1 cells was induced by C5a. The actions were efficiently inhibited by a specific antagonist against C5a. Our results implicated C5a in vessel formation and as a potent target for management of inflammatory diseases.
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- 2010
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105. Y-box binding protein-1 is a novel molecular target for tumor vessels.
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Takahashi M, Shimajiri S, Izumi H, Hirano G, Kashiwagi E, Yasuniwa Y, Wu Y, Han B, Akiyama M, Nishizawa S, Sasaguri Y, and Kohno K
- Subjects
- Angiogenesis Inhibitors therapeutic use, Brain Neoplasms blood supply, Brain Neoplasms chemistry, Cells, Cultured, DNA-Binding Proteins analysis, DNA-Binding Proteins antagonists & inhibitors, Endothelial Cells physiology, Glioblastoma blood supply, Glioblastoma chemistry, Humans, Neovascularization, Pathologic, Nuclear Proteins analysis, Nuclear Proteins antagonists & inhibitors, Y-Box-Binding Protein 1, DNA-Binding Proteins physiology, Neoplasms blood supply, Nuclear Proteins physiology
- Abstract
Y-box binding protein-1 (YB-1) is a member of the cold shock protein family and functions in transcription and translation. Many reports indicate that YB-1 is highly expressed in tumor cells and is a marker for tumor aggressiveness and clinical prognosis. Here, we show clear evidence that YB-1 is expressed in the angiogenic endothelial cells of various tumors, such as glioblastoma, esophageal cancer, gastric cancer, colon cancer, and lung cancer, as well as in tumor cells. YB-1 was highly expressed in glomeruloid microvascular endothelial cells of brain tumors and microvessels in the desmoplastic region around multiple solid tumors. On the other hand, no or low YB-1 expression was observed in normal angiogenic endothelial cells from fetal kidney, newborn lung, and placenta. The endothelial cells in inflammatory regions of granulomas were also weakly labeled. Knockdown of YB-1 expression by small-interfering RNA induced G1 cell cycle arrest and inhibited the growth of human umbilical vein endothelial cells stimulated by growth factors. Taken together, YB-1 plays an important role in the growth of not only tumor cells but also tumor-associated endothelial cells, suggesting that YB-1 is a promising target for cancer therapy.
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- 2010
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106. [Case of testicular carcinoid].
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Guo X, Yamada S, Wang KY, Shimajiri S, and Sasaguri Y
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- Adult, Humans, Immunohistochemistry, Male, Biomarkers, Tumor analysis, Carcinoid Tumor diagnosis, Carcinoid Tumor pathology, Testicular Neoplasms diagnosis, Testicular Neoplasms pathology
- Abstract
This is a case report of testicular carcinoid in a 37-year-old male who was admitted to hospital because of a gradual enlargement of the right testis. Macroscopically, the well-demarcated and solid lesion tumor was yellow-tan in color and confined to the testis. Microscopically, the proliferating tumor cells were arranged in a predominantly insular or lobular growth pattern admixed with cord- or ribbon-like areas. Teratomatous elements were not found. Grimelius stain revealed argyrophilic granules in the tumor cells, and, immunohistochemically, neuroendocrine markers and gastrointestinal hormones were diffusely postive in the tumor cells. From these features, we made a conclusive diagnosis of testicular carcinoid.
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- 2010
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107. Histology of fibrovascular membranes of proliferative diabetic retinopathy after intravitreal injection of bevacizumab.
- Author
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Kubota T, Morita H, Tou N, Nitta N, Tawara A, Satoh H, and Shimajiri S
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- Adult, Aged, Antibodies, Monoclonal, Humanized, Antigens, CD34 metabolism, Bevacizumab, Capillaries, Diabetic Retinopathy surgery, Endothelium, Vascular metabolism, Female, Fibrosis, Humans, Injections, Male, Middle Aged, Retinal Vessels metabolism, Vascular Endothelial Growth Factor A antagonists & inhibitors, Vascular Endothelial Growth Factor A metabolism, Vitrectomy, Vitreous Body, Angiogenesis Inhibitors administration & dosage, Antibodies, Monoclonal administration & dosage, Diabetic Retinopathy diagnosis, Diabetic Retinopathy drug therapy, Endothelium, Vascular pathology, Retinal Vessels pathology
- Abstract
Purpose: The purpose was to study the histology of the fibrovascular membranes in proliferative diabetic retinopathy (PDR) with an intravitreal injection of bevacizumab., Methods: Light and electron microscopic studies were performed on surgical specimens obtained during a pars plana vitrectomy from 6 PDR eyes after intravitreal injection of bevacizumab. The patients had preoperatively received no or scant retinal photocoagulations. The presence and distribution of CD34 was assessed as a marker of vascular endothelium using immunostaining. The presence of vascular endothelial growth factor was stained with a method of immunostaining. As controls, we examined 7 surgical specimens from 7 PDR eyes obtained during pars plana vitrectomy without bevacizumab therapy. All control patients had preoperatively received full or nearly full pan retinal photocoagulations., Results: Light microscopy showed that the CD34-positive vascular endothelial cells formed capillarylike structures in the fibrovascular membranes of all 13 PDR eyes. Vascular endothelial growth factor was positively stained in the vascular endothelium of both groups; however, the number of vascular endothelial growth factor-positive vascular endothelial cells significantly decreased in the fibrovascular membranes with intravitreal injection of bevacizumab. Electron microscopy showed the newly formed vascular endothelial cells with junctional complex in both groups., Conclusion: The vascular endothelial cells with decreased expression of vascular endothelial growth factor are still present in the fibrovascular membranes of patients with PDR after intravitreal injection of bevacizumab.
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- 2010
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108. Involvement of mast cells in systemic sclerosis.
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Yukawa S, Yamaoka K, Sawamukai N, Shimajiri S, Saito K, and Tanaka Y
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- Adaptive Immunity, Animals, Arthritis, Rheumatoid immunology, Benzamides, Disease Models, Animal, Female, Humans, Hypertension, Pulmonary immunology, Imatinib Mesylate, Immunity, Innate, Male, Mice, Middle Aged, Piperazines administration & dosage, Protein Kinase Inhibitors administration & dosage, Pyrimidines administration & dosage, Mast Cells immunology, Scleroderma, Systemic immunology, Scleroderma, Systemic therapy
- Abstract
Systemic sclerosis is characterized by tissue fibrosis, obliterative microangiopathy and immune abnormalities. The etiology of SSc is largely unknown and is known to be resistant to existing corticosteroid and immunosuppressive drugs. Therefore, establishment of a treatment strategy especially for SSc patients with organ involvement is strongly desired. Mast cells are widely recognized as effector cells in allergic disorders and other IgE-mediated immune responses. However, recently, mast cells have become known to play a role in bridging innate immunity and adaptive immunity. Additionally, there is growing evidence of mast cell to be involved in pathogenesis of rheumatoid arthritis, and is expected as a novel therapeutic target. We describe here the role of mast cell in SSc pathology and suggest as a novel therapeutic target.
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- 2010
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109. Pulmonary capillary hemangiomatosis in chronic cardiac failure due to aortic stenosis.
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Wang KY, Tanimoto A, Inenaga T, Yamada S, Shimajiri S, Ding Y, Guo X, and Sasaguri Y
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- Aortic Valve Stenosis pathology, Autopsy, Cardiomegaly etiology, Cardiomegaly pathology, Chronic Disease, Death, Sudden, Cardiac etiology, Fatal Outcome, Female, Heart Failure pathology, Heart Failure therapy, Heart Ventricles pathology, Hemangioma, Capillary pathology, Humans, Lung Neoplasms pathology, Pulmonary Edema etiology, Pulmonary Edema pathology, Renal Dialysis, Aortic Valve Stenosis complications, Heart Failure etiology, Hemangioma, Capillary etiology, Lung Neoplasms etiology
- Abstract
We report a case of pulmonary capillary hemangiomatosis in a patient with aortic stenosis. An 86-year-old Japanese female with chronic heart failure due to aortic stenosis suddenly died during hemodialysis. At an autopsy, severe aortic stenosis and cardiomegaly with both left and right ventricular hypertrophy were noted. In the lung, a diffuse proliferation of capillaries in the thickened alveolar septum and collections of hemosiderin-laden macrophages in the alveolar space were observed. These indicated that long-standing passive congestion from aortic stenosis might result in the occurrence of pulmonary capillary hemangiomatosis.
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- 2009
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110. Stromal luteoma and nodular hyperthecosis of the bilateral ovaries associated with atypical endometrial hyperplasia of the uterus.
- Author
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Yamada S, Tanimoto A, Wang KY, Shimajiri S, and Sasaguri Y
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- Endometrial Hyperplasia complications, Endometrial Hyperplasia metabolism, Female, Humans, Immunohistochemistry, Luteoma complications, Luteoma metabolism, Middle Aged, Ovarian Neoplasms complications, Ovarian Neoplasms metabolism, Polycystic Ovary Syndrome complications, Endometrial Hyperplasia pathology, Luteoma pathology, Ovarian Neoplasms pathology, Polycystic Ovary Syndrome pathology
- Published
- 2009
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111. Immunoreactivity of a monoclonal antibody against human placental alkaline phosphatase.
- Author
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Kou Y, Shimajiri S, and Hisaoka M
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- Adolescent, Cross Reactions, Humans, Male, Reverse Transcriptase Polymerase Chain Reaction, Antibodies, Monoclonal, Carcinoma, Embryonal metabolism, Immunohistochemistry methods, Proteins metabolism, Testicular Neoplasms metabolism
- Published
- 2009
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112. Ossifying synovial sarcoma.
- Author
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Hisaoka M, Matsuyama A, Shimajiri S, Akiba J, Kusano H, Hiraoka K, Shoda T, and Hashimoto H
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- Adult, Base Sequence, Humans, Immunohistochemistry, Male, Oncogene Proteins, Fusion, Reverse Transcriptase Polymerase Chain Reaction, Sarcoma, Synovial metabolism, Soft Tissue Neoplasms metabolism, Tomography, X-Ray Computed, Ossification, Heterotopic genetics, Ossification, Heterotopic pathology, Sarcoma, Synovial genetics, Sarcoma, Synovial pathology, Soft Tissue Neoplasms genetics, Soft Tissue Neoplasms pathology
- Abstract
Although intralesional calcification is a common finding of synovial sarcoma, ossification is an unusual phenomenon in such a soft tissue sarcoma. Here we report a case of ossifying synovial sarcoma arising in the back of a young adult man. Microscopically, the tumor was composed of spindle or oval cells together with foci of atypical polygonal cells in small nests or cord-like structures, displaying an epithelial appearance. In addition, extensive osteoid or woven bone formation was present in the tumor, resembling extraskeletal osteosarcoma. However, an SS18-SSX1 fusion gene transcript was detected by reverse transcription-polymerase chain reaction, supporting the diagnosis of biphasic synovial sarcoma. The osteogenic phenotype of the tumor cells was further demonstrated by an intense immunohistochemical expression of Runx2, a key transcription factor involved in the regulation of osteoblastic differentiation. The current case suggests the diagnostic utility of the molecular detection of a tumor type-specific fusion gene and expands the phenotypic plasticity of the soft tissue sarcoma of uncertain differentiation.
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- 2009
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113. DNA-based polymerase chain reaction for detecting FUS-CREB3L2 in low-grade fibromyxoid sarcoma using formalin-fixed, paraffin-embedded tissue specimens.
- Author
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Matsuyama A, Hisaoka M, Shimajiri S, and Hashimoto H
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- Adult, Amino Acid Sequence, Base Sequence, Basic-Leucine Zipper Transcription Factors genetics, Female, Fibroma pathology, Humans, Male, Middle Aged, Molecular Sequence Data, RNA-Binding Protein FUS genetics, Reverse Transcriptase Polymerase Chain Reaction, Sarcoma pathology, Sensitivity and Specificity, Basic-Leucine Zipper Transcription Factors biosynthesis, Fibroma diagnosis, Polymerase Chain Reaction methods, RNA-Binding Protein FUS biosynthesis, Sarcoma diagnosis
- Abstract
Detection of the tumor-specific fusion gene, FUS-CREB3L2, is useful in the diagnosis of low-grade fibromyxoid sarcoma (LGFMS), especially by reverse transcription (RT)-polymerase chain reaction (PCR) using formalin-fixed, paraffin-embedded (FFPE) tumor tissues. Because FUS and CREB3L2 gene segments with their break points within exons are fused without interposed introns in many LGFMSs, DNA-based PCR may also be applicable to detect the FUS-CREB3L2 fusion gene using FFPE specimens. In this study, DNA and RNA were extracted from FFPE specimens of 16 and 19 LGFMSs, respectively, and PCR and RT-PCR were performed using primers that specifically amplify most of the junctional regions of the FUS-CREB3L2 fusion gene. RT-PCR analysis revealed FUS-CREB3L2 fusion transcripts in 16 of the 19 (84%) LGFMSs. In 14 informative examples, including 11 with detectable fusion transcripts by RT-PCR, DNA-based PCR amplified the FUS-CREB3L2 fusion gene in 9 (64%) examples. Nucleotide sequence analysis confirmed that PCR products were identical to the respective RT-PCR products in 8 cases. Although the sensitivity was not as high as RT-PCR, DNA-based PCR can be used as an alternative molecular approach for detecting the FUS-CREB3L2 fusion gene.
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- 2008
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114. Matrix metalloproteinase 12 accelerates the initiation of atherosclerosis and stimulates the progression of fatty streaks to fibrous plaques in transgenic rabbits.
- Author
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Yamada S, Wang KY, Tanimoto A, Fan J, Shimajiri S, Kitajima S, Morimoto M, Tsutsui M, Watanabe T, Yasumoto K, and Sasaguri Y
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- Animals, Animals, Genetically Modified, Aorta, Thoracic metabolism, Aorta, Thoracic pathology, Atherosclerosis etiology, Atherosclerosis pathology, Basement Membrane metabolism, Cell Movement, Cholesterol, Dietary, Humans, Macrophages pathology, Macrophages physiology, Male, Matrix Metalloproteinase 12 genetics, Myocytes, Smooth Muscle metabolism, Myocytes, Smooth Muscle pathology, Rabbits, Atherosclerosis metabolism, Matrix Metalloproteinase 12 metabolism
- Abstract
Whether fatty streaks are directly followed by fibrous plaque formation in atherosclerosis remains controversial. Disruption of the basement membrane and elastic layers is thought to be essential for this process. Matrix metalloproteinase 12 (MMP-12) can degrade a broad spectrum of substrates, but the role of MMP-12 in the early stage of atherosclerosis is unclear. To investigate MMP-12 function in the initiation and progression of atherosclerosis, we investigated macrophage migration and elastolysis in relation to fatty streaks in human MMP-12 transgenic (hMMP-12 Tg) rabbits. Fatty streaks in hMMP-12 Tg rabbits fed a 1% cholesterol diet for 6 weeks (cholesterol-induced model of atherosclerosis) were more pronounced and were associated with more significant degradation of the internal elastic layer compared with wild-type (WT) animals. Numbers of infiltrating macrophages and smooth muscle cells in the lesions were increased in hMMP-12 Tg compared with WT animals. In both cuff- and ligation-induced models of atherosclerosis, smooth muscle cell-predominant atherosclerotic lesions were elevated with significant elastolysis of the internal elastic lamina in Tg compared with WT animals; "microelastolytic sites" were recognized before formation of the neointima in the cuff model only. These results indicate that MMP-12 may be critical to the initiation and progression of atherosclerosis via degradation of the elastic layers and/or basement membrane. Therefore, a specific MMP-12 inhibitor might prove useful for the treatment of progressive atherosclerosis.
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- 2008
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115. Aging gracefully: a retrospective analysis of functional status in Okinawan centenarians.
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Willcox DC, Willcox BJ, Shimajiri S, Kurechi S, and Suzuki M
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- Aged, 80 and over, Aging physiology, Female, Humans, Japan, Male, Physical Examination methods, Retrospective Studies, Time Factors, Activities of Daily Living psychology, Aging psychology, Cognition physiology, Geriatric Assessment methods, Health Status
- Abstract
Objective: This study retrospectively explored the late-life functional status of Okinawan centenarians., Methods: Activities of daily living were measured retrospectively at five time points (10, 5, 3, and 1 year prior and present) for 22 centenarians in relation to seven physical, two sensory, and two cognitive functions using the Inoue Index., Results: In all, 82% of individuals were still functioning independently at a mean age of 92 years and almost two-thirds were still functioning independently at a mean age of 97 years., Conclusion: Preliminary analyses suggest high functional status in Okinawan centenarians throughout their 90 s. The genetic and environmental factors contributing to this successful aging phenomenon deserve further investigation.
- Published
- 2007
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116. Molecular detection of SS18-SSX fusion gene transcripts by cRNA in situ hybridization in synovial sarcoma using formalin-fixed, paraffin-embedded tumor tissue specimens.
- Author
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Kanemitsu S, Hisaoka M, Shimajiri S, Matsuyama A, and Hashimoto H
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- Adolescent, Adult, Aged, Biomarkers, Tumor analysis, Female, Formaldehyde chemistry, Humans, In Situ Hybridization methods, Male, Middle Aged, Paraffin Embedding, RNA Probes chemistry, Sarcoma, Synovial chemistry, Sarcoma, Synovial pathology, Neoplasm Proteins genetics, Oncogene Fusion, Proto-Oncogene Proteins genetics, RNA, Complementary analysis, Repressor Proteins genetics, Sarcoma, Synovial genetics, Transcription, Genetic
- Abstract
SS18-SSX fusion genes resulting from a chromosomal translocation t(X;18)(p11.2;q11.2) are a genetic hallmark of synovial sarcoma. Although such cytogenetic or molecular aberrations have mostly been detected by fluorescence in situ hybridization or reverse transcription-polymerase chain reaction, the expression of SS18-SSX has been poorly investigated at a cellular or tissue level. In this study, biotinylated tyramide (BT)-based in situ hybridization (ISH) was performed to detect SS18-SSX transcripts using formalin-fixed, paraffin-embedded tissues from 15 synovial sarcomas. Digoxigenin-labeled cRNA probes flanking the fusion points of SS18-SSX1 and SS18-SSX2 were generated by in vitro transcription, and hybridized signals were detected by a streptavidin-biotin complex method after chemical enhancement with BT. The localizations of signals were compared with the immunohistochemical expressions of epithelial or neuroectodermal markers and those of cell adhesion including cytokeratins (CAM5.2, AE1/AE3, CK7), epithelial membrane antigen, E-cadherin, beta-catenin, c-erbB-2 (HER2/neu), CD56, and claudin-1. The ISH signals of the SS18-SSX transcripts were identified in 13 synovial sarcomas, and their fusion types correlated with those determined by reverse transcription-polymerase chain reaction. In biphasic tumors, the ISH signals tended to localize to epithelial areas, whereas spindle-cell areas or monophasic fibrous tumors showed a less intense or focal expression pattern. Notably, the expression patterns of AE1/AE3, CK7, and c-erbB-2 often colocalized with the ISH signals (7 of 11 cases positive for each marker). Our results suggest that BT-based ISH can be used as a molecular technique for the detection of SS18-SSX using formalin-fixed, paraffin-embedded tissues.
- Published
- 2007
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117. Molecular detection of FUS-CREB3L2 fusion transcripts in low-grade fibromyxoid sarcoma using formalin-fixed, paraffin-embedded tissue specimens.
- Author
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Matsuyama A, Hisaoka M, Shimajiri S, Hayashi T, Imamura T, Ishida T, Fukunaga M, Fukuhara T, Minato H, Nakajima T, Yonezawa S, Kuroda M, Yamasaki F, Toyoshima S, and Hashimoto H
- Subjects
- Adolescent, Adult, Female, Fibrosarcoma pathology, Formaldehyde, Histological Techniques, Humans, Male, Middle Aged, Paraffin, Reverse Transcriptase Polymerase Chain Reaction, Soft Tissue Neoplasms pathology, Cyclic AMP Response Element-Binding Protein genetics, Fibrosarcoma genetics, Nerve Tissue Proteins genetics, Oncogene Proteins, Fusion genetics, Soft Tissue Neoplasms genetics, Transcription, Genetic
- Abstract
A diagnosis of low-grade fibromyxoid sarcoma (LGFMS) remains problematic because of its bland-looking histologic features that can be potentially confused with other benign or low-grade fibromyxoid lesions. Recent cytogenetic and molecular analyses have shown that most LGFMSs have a characteristic chromosomal abnormality, t(7;16)(q33;p11), resulting in the FUS-CREB3L2 fusion gene. However, such assays have only rarely been used to analyze formalin-fixed, paraffin-embedded tumor samples. In the present study, we conducted a reverse transcription-polymerase chain reaction assay to detect the FUS-CREB3L2 fusion transcripts using formalin-fixed, paraffin-embedded tumor tissue specimens from 16 LGFMSs including 3 cases with giant collagen rosettes. The primers were newly designed to specifically amplify most of the junctional regions of the FUS-CREB3L2 fusion gene transcripts previously reported. The FUS-CREB3L2 fusion gene transcripts were detected in 14/16 (88%) cases of LGFMS. A nucleotide sequence analysis of the PCR products revealed that different portions of the FUS exon 6 or 7 were fused with various sites of the CREB3L2 exon 5, resulting in 12 different nucleotide sequences. We also tested a primer set to detect the FUS-CREB3L1 fusion transcript, which is a rare variant of the gene fusion in LGFMS, although no PCR products were identified in any case. The FUS-CREB3L2 fusion transcripts were not detected in any of the 123 other soft-tissue tumors, including desmoid-type fibromatoses, myxofibrosarcomas, soft-tissue perineuriomas, and congenital or adult fibrosarcomas. These data suggest that our reverse transcription-polymerase chain reaction assay is a reliable method to detect FUS-CREB3L2, which can thus help in accurately diagnosing LGFMS.
- Published
- 2006
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118. [A case of partial response in liver metastatic lesion from gastric endocrine cell carcinoma treated with TS-1].
- Author
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Hanada N, Inoue K, Osako T, Kuriwaki K, Shimajiri S, and Mochinaga M
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- Aged, Carcinoma, Small Cell secondary, Carcinoma, Small Cell surgery, Combined Modality Therapy, Drug Administration Schedule, Drug Combinations, Female, Gastrectomy, Humans, Remission Induction, Stomach Neoplasms pathology, Stomach Neoplasms surgery, Antimetabolites, Antineoplastic administration & dosage, Carcinoma, Small Cell drug therapy, Liver Neoplasms drug therapy, Liver Neoplasms secondary, Oxonic Acid administration & dosage, Stomach Neoplasms drug therapy, Tegafur administration & dosage
- Abstract
Endocrine cell carcinoma (ECC) of the stomach is a rare pathological type with a poor outcome. Standard chemotherapy has not yet been established. We experienced a case of partial response in the liver metastasis from the gastric ECC treated with TS-1. The patient was a 68-year-old female. An upper GI series and gastroendoscopy revealed pyrolus stenosis and CT-scan showed liver metastasis. A non-curative total gastrectomy was performed to allow oral intake. Since the histopathological examination revealed that tumor cells were partially positive for chromogranin A on immunohistochemical study, we diagnosed tumor ECC of the stomach. The patient with liver metastasis was treated using TS-1, and CT-scan showed a remarkable decrease (PR) in the metastatic lesion. TS-1 administration can improve the clinical outcome for ECC of the stomach.
- Published
- 2006
119. GM-CSF activates RhoA, integrin and MMP expression in human monocytic cells.
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Kohno Y, Tanimoto A, Cirathaworn C, Shimajiri S, Tawara A, and Sasaguri Y
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- Becaplermin, Blotting, Northern, Cell Line, Cell Movement drug effects, Chemokine CCL2 pharmacology, Genes, Reporter, Granulocyte-Macrophage Colony-Stimulating Factor physiology, Humans, Luciferases, Matrix Metalloproteinases genetics, Monocytes metabolism, Platelet-Derived Growth Factor pharmacology, Proto-Oncogene Proteins c-sis, RNA, Messenger metabolism, Granulocyte-Macrophage Colony-Stimulating Factor pharmacology, Integrins biosynthesis, Matrix Metalloproteinases biosynthesis, Monocytes drug effects, rhoA GTP-Binding Protein biosynthesis
- Abstract
Monocyte migration is one of the key events occurring in the early stage of atherosclerosis. This process includes monocytic adhesion to and penetration through the arterial intima. In such an environment, many factors stimulate the monocytes to enhance integrin activation and extracellular matrix degradation. To investigate the coordinative operation of these two events in relation to monocyte migration, we paid particular attention to the effects of granulocyte-macrophage colony-stimulating factor (GM-CSF) on monocytes in terms of RhoA activation and matrix metalloproteinase (MMP) expression. RhoA and integrin clustering were activated by GM-CSF, monocyte chemoattractant protein-1 (MCP-1) and platelet-derived growth factor-BB (PDGF-BB) in human monocytic cell lines. Furthermore, enhancement of migration was observed with stimulation by MCP-1 and PDGF-BB. Granulocyte-macrophage colony-stimulating factor did not enhance the migration, even though it activated RhoA and integrin. However, GM-CSF is known to stimulate monocytes to express MCP-1, suggesting the presence of an indirect mechanism for GM-CSF-mediated migratory activity. In contrast, only GM-CSF enhanced the expression of MMP-1 and MMP-9. These results provide evidence that GM-CSF has multiple functions enhancing monocytic migration via RhoA and integrin activation, and via MMP expression.
- Published
- 2004
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120. Histidine decarboxylase expression in pancreatic endocrine cells and related tumors.
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Tanimoto A, Matsuki Y, Tomita T, Sasaguri T, Shimajiri S, and Sasaguri Y
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- Adult, Aged, Biomarkers, Tumor metabolism, Female, Fluorescent Antibody Technique, Indirect, Humans, Immunoenzyme Techniques, Insulinoma pathology, Islets of Langerhans pathology, Male, Middle Aged, Pancreatic Neoplasms pathology, Histidine Decarboxylase metabolism, Insulinoma enzymology, Islets of Langerhans enzymology, Pancreatic Neoplasms enzymology
- Abstract
Histidine decarboxylase (HDC) is an enzyme for decarboxylating l-histidine to histamine and is expressed in various types of cells including neuroendocrine tumors. Recent findings have demonstrated a high percentage of HDC immunoreactivity in many neuroendocrine tumors, including carcinoid tumors, small cell carcinomas of the lung, pheochromocytomas, and medullary carcinomas of the thyroid. HDC immunostaining was applied to pancreatic islet cells and related tumors to explore possible expression of HDC as a wide spectrum marker for neuroendocrine differentiation. A total of 24 cases (22 pancreatic endocrine neoplasms, one small cell carcinoma of the pancreas, and one mixed exocrine-endocrine carcinoma) along with normal pancreatic tissue were immunostained with the anti-HDC antibody. In a normal pancreas, a double immunostaining revealed possible colocalization of HDC with glucagon- or insulin-positive cells in the islets. Seventeen of 22 pancreatic endocrine neoplasms (77%) were found to be positive for HDC, and no distinct relation to hormonal activity was observed. One small cell carcinoma was strongly positive to HDC. One non-functional tumor with mixed exocrine and endocrine components showed a diffuse positive immunostaining for HDC, and some neoplastic glucagon- or somatostatin (SRIF)-positive cells coexpressed HDC. In conclusion, we demonstrated that the majority of pancreatic endocrine tumors expressed HDC, and we suggest that HDC is a wider new marker for neuroendocrine differentiation.
- Published
- 2004
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121. Inflammatory myofibroblastic tumor with predominant anaplastic lymphoma kinase-positive cells lacking a myofibroblastic phenotype.
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Hisaoka M, Shimajiri S, Matsuki Y, Meis-Kindblom JM, Kindblom LG, Li XQ, Wang J, and Hashimoto H
- Subjects
- Adult, Anaplastic Lymphoma Kinase, Child, Child, Preschool, DNA analysis, Female, Fibroblasts ultrastructure, Granuloma, Plasma Cell pathology, Humans, Immunohistochemistry, Infant, Male, Myocytes, Smooth Muscle ultrastructure, Oncogene Proteins, Fusion genetics, Oncogene Proteins, Fusion metabolism, Phenotype, Protein-Tyrosine Kinases genetics, Receptor Protein-Tyrosine Kinases, Reverse Transcriptase Polymerase Chain Reaction, Fibroblasts enzymology, Granuloma, Plasma Cell enzymology, Myocytes, Smooth Muscle enzymology, Protein-Tyrosine Kinases metabolism
- Abstract
Inflammatory myofibroblastic tumor (IMT), synonymously referred to as inflammatory pseudotumor, is a distinctive mesenchymal lesion composed of spindle cells displaying morphological features of myofibroblasts admixed with considerable numbers of inflammatory cells. Recent genetic and molecular studies have shown that a subset of IMT is characterized by the expression of altered anaplastic lymphoma kinase (ALK) protein mostly resulting from rearrangements of the ALK gene such as TPM3-ALK, TPM4-ALK and CLTC-ALK fusion genes. We analyzed the ALK status in nine cases of IMT arising in various anatomical locations. Six cases showed immunohistochemical expression of the ALK protein, and two ALK-positive lesions examined by reverse transcription-polymerase chain reaction and a subsequent sequencing analysis harbored the TPM4-ALK fusion gene. Of note, the majority of ALK-positive tumor cells in four of the six lesions lacked the coexpression of myogenic markers including alpha-smooth muscle actin, a cytoskeletal protein indicating myofibroblastic differentiation, whereas a substantial number of tumor cells in the remaining two cases coexpressed ALK and alpha-smooth muscle actin and/or desmin. In an ultrastructural study of the lesion with predominant ALK-positive/actin-negative cells, spindle cells failed to demonstrate features of myofibroblasts such as intracytoplasmic bundles of thin filaments and dense bodies. The current findings suggest that ALK-positive cells in IMT are not always myofibroblastic but might be immature primitive mesenchymal cells.
- Published
- 2003
- Full Text
- View/download PDF
122. [Small cell carcinoma of the prostate successfully treated with combined chemotherapy and radiotherapy: a case report].
- Author
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Ishizu K, Tsushimi M, Shimajiri S, Hamada T, Sasaguri Y, and Naito K
- Subjects
- Combined Modality Therapy, Humans, Male, Middle Aged, Carcinoma, Small Cell drug therapy, Carcinoma, Small Cell radiotherapy, Prostatic Neoplasms drug therapy, Prostatic Neoplasms radiotherapy
- Abstract
A 49-year-old man complained of dysuria and pollakisuria. The prostate was enlarged, and the serum level of prostate specific antigen was within the normal range. Under the diagnosis of benign prostatic hypertrophy, transurethral resection of the prostate was performed. Unexpectedly, histopathological examination of the resected tissues revealed pure small cell carcinoma. The serum level of progastrin-releasing peptide (ProGRP) was slightly elevated. The cancer was clinically diagnosed as stage C. Pelvic radiotherapy combined with chemotherapy using cisplatin (CDDP) and etoposide (VP-16) was started according to the treatment for limited small cell cancer of the lung. After one month, the serum level of ProGRP decreased to the normal range. After four months, the prostate was reduced in size without any findings of metastases on computed tomography, and prostate biopsy revealed no viable cancer cells.
- Published
- 2002
123. Extraskeletal myxoid chondrosarcoma: a clinicopathologic, immunohistochemical, and molecular analysis of 18 cases.
- Author
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Okamoto S, Hisaoka M, Ishida T, Imamura T, Kanda H, Shimajiri S, and Hashimoto H
- Subjects
- Adult, Aged, Artificial Gene Fusion, Base Sequence, Biomarkers, Tumor analysis, Chondrosarcoma chemistry, Chondrosarcoma genetics, Chondrosarcoma surgery, DNA Primers chemistry, DNA-Binding Proteins analysis, DNA-Binding Proteins genetics, Female, Humans, Immunohistochemistry, Male, Middle Aged, Molecular Sequence Data, Nuclear Proteins analysis, Nuclear Proteins genetics, RNA, Messenger analysis, RNA, Messenger metabolism, RNA, Neoplasm analysis, Receptors, Steroid, Receptors, Thyroid Hormone, Reverse Transcriptase Polymerase Chain Reaction, Soft Tissue Neoplasms chemistry, Soft Tissue Neoplasms genetics, Soft Tissue Neoplasms surgery, Transcription Factors analysis, Transcription Factors genetics, Chondrosarcoma pathology, Nerve Tissue Proteins, Soft Tissue Neoplasms pathology, TATA-Binding Protein Associated Factors
- Abstract
Extraskeletal myxoid chondrosarcoma (EMCS) is an uncommon clinicopathologically well-defined tumor, but its pathogenesis and biologic behavior are poorly understood. We reviewed 18 cases of EMCS to verify clinicopathologic features and immunohistochemical profiles together with molecular detection of the tumor-specific fusion genes. The tumors were located mainly in the proximal extremities and limb girdles (72%). Two tumors arose at unusual anatomic sites: the finger and the hip joint. Nine of the 17 followed-up patients were alive and disease free, 4 were alive with recurrences and/or metastases, and 4 died of the tumor. Fifteen tumors showed typical features of EMCS, and 3 had hypercellular areas in addition to conventional EMCS areas. The tumors were variably immunoreactive for S-100 protein (50%), NSE (89%), peripherin (60%), and synaptophysin (22%). Chromogranin A and some epithelial markers (AE1/AE3, CAM5.2, and epithelial membrane antigen) were entirely negative. Frequent expressions of the neural/neuroendocrine markers suggest possible neural/neuroendocrine differentiation in at least some EMCSs, in addition to chondroid differentiation. In a reverse-transcription polymerase chain reaction (RT-PCR) assay using paraffin-embedded specimens, EWS-CHN or TAF2N-CHN fusion gene transcripts characteristic of EMCS could be detected in 15 (83%) of the 18 cases: EWS-CHN type 1 in 11 cases, EWS-CHN type 2 in 1, and TAF2N-CHN in 3. Three fusion-negative cases included 2 conventional EMCSs and 1 considered a "cellular" variant of the tumor. None of 30 other soft tissue and bone tumors with myxoid or chondroid morphology that we examined contained these fusion genes. Thus, RT-PCR detection of EWS-CHN or TAF2N-CHN fusion gene using archival paraffin-embedded tissue is a feasible and useful ancillary technique for the diagnosis of EMCS., (Copyright 2001 by W.B. Saunders Company)
- Published
- 2001
- Full Text
- View/download PDF
124. Detection of COL1A1-PDGFB fusion transcripts in dermatofibrosarcoma protuberans by reverse transcription-polymerase chain reaction using archival formalin-fixed, paraffin-embedded tissues.
- Author
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Wang J, Hisaoka M, Shimajiri S, Morimitsu Y, and Hashimoto H
- Subjects
- Adolescent, Adult, Chromosome Mapping, Collagen genetics, Collagen Type I, alpha 1 Chain, Dermatofibrosarcoma pathology, Female, Humans, Male, Middle Aged, Platelet-Derived Growth Factor genetics, Polymerase Chain Reaction, Skin Neoplasms pathology, Chromosomes, Human, Pair 17, Chromosomes, Human, Pair 22, Dermatofibrosarcoma genetics, Oncogene Proteins, Fusion genetics, Skin Neoplasms genetics, Transcription, Genetic, Translocation, Genetic
- Abstract
The reciprocal translocation t(17;22)(q22;q13) and a supernumerary ring chromosome, r(17;22), derived from the translocation, have been shown to be highly characteristic of dermatofibrosarcoma protuberans (DFSP). Its consequence is a fusion of two genes, a collagen type I alpha 1 gene (COL1A1) and platelet-derived growth factor B-chain gene (PDGFB). The COL1A1-PDGFB fusion gene, is expected to be a diagnostic molecular assay. However, previous studies on this subject were mostly based on frozen tissue specimens or cultured tumor cells. In this present study, the investigators conducted a reverse transcription (RT)-polymerase chain reaction (PCR) assay to detect the COL1A1-PDGFB fusion transcripts using archival formalin-fixed, paraffin-embedded tumor specimens from 12 patients with DFSP. To amplify the fusion transcripts, a specific COL1A1 forward and PDGFB reverse primers were designed for single step PCR. The COL1A1-PDGFB fusion transcripts could be detected in 10 of 12 paraffin-embedded DFSP tumor specimens (83%). Subsequent sequence analysis using the PCR products confirmed that the detected messages were derived from gene fusions composed of PDGFB exon 2 and different regions of the COL1A1 gene (exon 8, 10, 22, 24, 32, 38, 45 or 46). Two samples of Bednar tumor included in this series also contained the fusion transcripts. In sample of DFSP with fibrosarcomatous transformation, the COL1A1-PDGFB could not be detected in the fibrosarcoma areas of the third recurrence, though the chimeric transcripts were identified in the ordinary DFSP areas of the first recurrence. No fusion transcripts could be amplified in non-DFSP lesions, including 10 dermatofibromas and 9 malignant fibrous histiocytomas. These results indicate that this molecular assay could be applied to archival formalin-fixed, paraffin-embedded tumor tissues as a diagnostic aid for DFSP.
- Published
- 1999
- Full Text
- View/download PDF
125. Molecular detection of EWS-FLI1 chimeric transcripts in Ewing family tumors by nested reverse transcription-polymerase chain reaction: application to archival paraffin-embedded tumor tissues.
- Author
-
Hisaoka M, Tsuji S, Morimitsu Y, Hashimoto H, Shimajiri S, Komiya S, and Ushijima M
- Subjects
- Adolescent, Adult, Aged, Child, Child, Preschool, Exons, Female, Humans, In Situ Hybridization, Fluorescence, Infant, Male, Middle Aged, Oncogene Proteins, Fusion analysis, Paraffin, Polymerase Chain Reaction, Proto-Oncogene Protein c-fli-1, RNA-Binding Protein EWS, Sequence Analysis, DNA, Tissue Embedding, Transcription Factors analysis, Bone Neoplasms genetics, Chromosomes, Human, Pair 11, Chromosomes, Human, Pair 22, Oncogene Proteins, Fusion genetics, Sarcoma, Ewing genetics, Transcription Factors genetics, Translocation, Genetic
- Abstract
Chromosomal translocations generating unique chimeric genes are highly characteristic of specific sarcomas, and their use as diagnostic markers has been suggested. From a diagnostic pathologic point of view, detection of such cytogenetic or molecular aberrations applicable to routinely processed archival tissue specimens is considered a powerful tool for tumor diagnosis. To assess the feasibility and reliability of the molecular detection of the transcript originating from the chimeric gene in paraffin-embedded tumor specimens, we performed a nested reverse transcription-polymerase chain reaction (RT-PCR)-based assay to detect the EWS-FLI1 chimeric message in a series of Ewing family tumors. Of 24 paraffin-embedded tumor specimens from 23 cases analyzed, the chimeric message was detectable in 20 (83%) specimens from 20 cases (87%) by this nested RT-PCR assay, whereas none of 7 small round cell tumors not from this family (3 alveolar rhabdomyosarcomas, 2 neuroblastomas, 2 malignant lymphomas) showed detectable chimeric messages. In the sequence analysis of the PCR products, the amplified chimeric messages contained the junctions between exon 7 of the EWS gene and any one of exons 5, 6 and 8 of the FLI1 gene. The detection process was usually completed within 3 days, except for the subseqent sequence analysis. Our results endorse the use of this molecular assay as an ancillary technique in the diagnosis of Ewing family tumors using paraffin-embedded material.
- Published
- 1999
- Full Text
- View/download PDF
126. Detection of SYT-SSX fusion transcripts in synovial sarcoma by reverse transcription-polymerase chain reaction using archival paraffin-embedded tissues.
- Author
-
Tsuji S, Hisaoka M, Morimitsu Y, Hashimoto H, Shimajiri S, Komiya S, Ushijima M, and Nakamura T
- Subjects
- Adolescent, Adult, Base Sequence, Biomarkers, Tumor genetics, Female, Humans, Male, Middle Aged, Neoplasm Proteins genetics, Paraffin Embedding, Proteins genetics, Proto-Oncogene Proteins, Recombinant Fusion Proteins analysis, Repressor Proteins genetics, Reverse Transcriptase Polymerase Chain Reaction, Sarcoma, Synovial genetics, Sarcoma, Synovial mortality, Sarcoma, Synovial pathology, Survival Rate, Translocation, Genetic, Biomarkers, Tumor analysis, Neoplasm Proteins analysis, Proteins analysis, Repressor Proteins analysis, Sarcoma, Synovial chemistry
- Abstract
The reciprocal translocation t(X;18)(p11;q11) is known to be highly characteristic of synovial sarcoma, and its consequence, an SYT-SSX fusion gene, is expected to be a diagnostic molecular marker. In this study, we conducted a reverse transcription-polymerase chain reaction-based assay to detect the SYT-SSX fusion gene transcripts using archival formalin-fixed, paraffin-embedded tumor specimens from a series of 32 synovial sarcoma cases including 6 tumors found in unusual anatomical sites. The SYT-SSX fusion transcripts could be detected in 30 of 32 paraffin-embedded specimens (94%). A subsequent sequence analysis using the polymerase chain reaction products confirmed that the detected messages were derived from either the SYT-SSX1 (22 cases) or SYT-SSX2 (8 cases) fusion gene. Of 23 SYT-SSX-positive monophasic tumors, 16 tumors had an SYT-SSX1 fusion transcript. Fusion transcripts were detectable in all the 7 biphasic tumors analysed, one of which had an SYT-SSX2 fusion transcript. All of the six tumors at unusual locations (lung; 3, metastasis to the abdominal cavity from a tumor of retroperitoneal origin; 1, sacral region; 1, iliopsoas muscle; 1) contained detectable messages. Our results indicate that this molecular assay can be applied to archival formalin-fixed, paraffin-embedded tumor tissues as a feasible and reliable molecular technique for the diagnosis of synovial sarcoma.
- Published
- 1998
- Full Text
- View/download PDF
127. Altered membrane skeleton of red blood cells participates in cadmium-induced anemia.
- Author
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Hamada T, Tanimoto A, Arima N, Ide Y, Sasaguri T, Shimajiri S, and Sasaguri Y
- Subjects
- Acanthocytes pathology, Anemia, Hypochromic blood, Animals, Erythrocyte Count, Erythrocyte Membrane ultrastructure, Erythrocytes, Abnormal pathology, Female, Hemolysis, Male, Microscopy, Electron, Osmotic Fragility, Rats, Anemia, Hypochromic chemically induced, Cadmium toxicity, Erythrocyte Membrane drug effects
- Abstract
Poikilocytosis of red blood cells (RBCs) was observed to be associated with anemia in rats given subcutaneous injections of cadmium (Cd). Phase-contrast light and scanning electron microscopic examinations revealed that acanthocytes appeared in the early stages of administration, and that the number of RBC fragments increased later. Ultrastructural analysis of RBC ghosts by negative staining demonstrated that the normal lattice structure of the membrane skeleton was abolished. The osmotic fragility curve of the Cd-exposed RBCs disclosed that most of the cells were less fragile than control RBCs. These data indicate that the RBC membrane skeleton is initially altered by Cd-exposure, followed by deformation of the cell, thus promoting intrasplenic hemolysis, and resulting in anemia.
- Published
- 1998
- Full Text
- View/download PDF
128. Detection of TLS/FUS-CHOP fusion transcripts in myxoid and round cell liposarcomas by nested reverse transcription-polymerase chain reaction using archival paraffin-embedded tissues.
- Author
-
Hisaoka M, Tsuji S, Morimitsu Y, Hashimoto H, Shimajiri S, Komiya S, and Ushijima M
- Subjects
- Adolescent, Adult, Chromosomes, Human, Pair 12 ultrastructure, Chromosomes, Human, Pair 16 ultrastructure, Female, Humans, Liposarcoma chemistry, Liposarcoma classification, Liposarcoma, Myxoid genetics, Male, Middle Aged, Paraffin Embedding, Soft Tissue Neoplasms chemistry, Transcription Factor CHOP, Biomarkers, Tumor genetics, CCAAT-Enhancer-Binding Proteins, Chromosomes, Human, Pair 12 genetics, Chromosomes, Human, Pair 16 genetics, Liposarcoma genetics, Liposarcoma, Myxoid chemistry, Neoplasm Proteins genetics, Nuclear Proteins genetics, Oncogene Proteins, Fusion genetics, RNA, Messenger analysis, RNA, Neoplasm analysis, RNA-Binding Protein FUS, Reverse Transcriptase Polymerase Chain Reaction, Soft Tissue Neoplasms genetics, Translocation, Genetic
- Abstract
The reciprocal translocation t(12;16)(q13;p11) has been shown to be highly characteristic of myxoid and round cell subtypes of liposarcoma, and the TLS/FUS-CHOP fusion gene that resulted from the translocation is expected to be a diagnostic molecular marker of these sarcomas. In this study, we conducted a nested reverse transcription-polymerase chain reaction (RT-PCR)-based assay to detect the TLS/FUS-CHOP fusion gene transcripts using archival formalin-fixed, paraffin-embedded tumor specimens. Of 18 paraffin-embedded specimens from 16 myxoid and round cell liposarcoma cases, the fusion transcripts could be identified in 16 (89%) specimens from 15 (94%) cases. A sequence analysis using the PCR products confirmed that the detected messages were derived from either type I or type II TLS/FUS-CHOP fusion gene, the latter of which was predominant (80%). The results were consistent in primary and recurrent lesions of the same patients and in paraffin-embedded and snap-frozen samples from the same tumors. In two negative specimens, transcripts of the beta-actin gene could not be detected by RT-PCR, and intact mRNA including the fusion messages might have been degraded. No fusion transcripts were detected in snap-frozen or paraffin-embedded material of other types of tumors with myxoid morphology (seven myxoid malignant fibrous histiocytomas and four lipomas with myxoid change). These results indicate that this molecular assay can be applied to formalin-fixed, paraffin-embedded tumor tissues as a diagnostic aid for these subtypes of liposarcoma.
- Published
- 1998
- Full Text
- View/download PDF
129. Pathological study of splenomegaly associated with cadmium-induced anemia in rats.
- Author
-
Hamada T, Tanimoto A, Arima N, Ide Y, Sasaguri T, Shimajiri S, Murata Y, Wang KY, and Sasaguri Y
- Subjects
- Anemia blood, Anemia complications, Animals, Erythrocytes, Abnormal drug effects, Female, Male, Microscopy, Electron, Rats, Rats, Sprague-Dawley, Spleen ultrastructure, Splenomegaly chemically induced, Anemia chemically induced, Cadmium toxicity, Cadmium Poisoning complications, Splenomegaly pathology
- Abstract
Splenomegaly was observed both in male and female Sprague-Dawley rats after 1 week of exposure to CdCl2 (0.6 mg Cd/kg/day). Spleen weight reached about double that in controls by 8 weeks of Cd exposure. Histopathological examination of the enlarged spleen revealed that iron- and lipid-laden histiocytes were clustered in the periarterial lymphatic sheath, and the red pulp appeared to be expanded. It is noteworthy that electron microscopy revealed marked poikilocytosis and Heinz body formation in red blood cells (RBCs) in both the sinus and cord. Histiocytes were swollen by a granular substance in the cytoplasm and also many secondary lysosomes. These morphological findings indicate that degradation of damaged RBCs induced by exposure to Cd might be promoted in the spleen and possibly cause splenomegaly. This RBC damage-hemolysis-splenomegaly sequence is also considered to be associated with the etiology of Cd-induced anemia. In addition to the abnormal RBC degradation, nuclei of lymphocytes in the Cd-exposed spleen exhibited high electron density, consistent with a preapoptotic state suggesting the immunosuppressive effect of Cd.
- Published
- 1998
- Full Text
- View/download PDF
130. Apoptosis of human kidney 293 cells is promoted by polymerized cadmium-metallothionein.
- Author
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Hamada T, Sasaguri T, Tanimoto A, Arima N, Shimajiri S, Abe T, and Sasaguri Y
- Subjects
- Animals, Cadmium Chloride, Cell Line, Transformed, Chromatography, Gel, DNA chemistry, DNA drug effects, DNA ultrastructure, Dose-Response Relationship, Drug, Enzyme-Linked Immunosorbent Assay, Humans, Kidney, Kinetics, Metallothionein chemistry, Metallothionein isolation & purification, Microscopy, Electron, Rabbits, Apoptosis drug effects, Cadmium pharmacology, Chlorides pharmacology, Metallothionein pharmacology, Nucleosomes ultrastructure
- Abstract
Transformed human kidney cells (293 cells) exposed to 12.5 to 37.5 microM CdCl(2) showed apoptosis as confirmed by characteristic electron microscopic features, a ladder on gel electrophoresis of extracted DNA, and fragmentation of nucleosomes as detected by enzyme linked immunosorbent assay (ELISA). Higher concentrations of Cd were less effective in inducing apoptosis. Furthermore, addition of the protein extract from the serum-free medium used for Cd-exposure promoted apoptosis exhibiting the same features as that after Cd-exposure. The apoptosis induced by the protein was dose-dependent. The molecular weight of the protein (Cd-protein) was shown to be 40 kDa by gel filtration. Two-dimensional electrophoresis revealed the Cd-protein as a single spot with a molecular weight of 6 kDa and pI of 4.5. Competitive ELISA showed that the Cd-protein reacted with anti-metallothionein antibody. The present findings suggest that apoptosis is induced not only by Cd itself but also by polymerized metallothionein molecules (MT) released from cells into the medium.
- Published
- 1996
- Full Text
- View/download PDF
131. Expression of PDGF and C-myc in atherosclerotic lesions in cholesterol-fed chicken. Immunohistochemical and in situ hybridization study.
- Author
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Toda T, Tamamoto T, Shimajiri S, Sadi AM, Nakashima Y, and Takei H
- Subjects
- Animals, Chickens, Diet, Atherogenic, Gene Expression, Genes, myc, In Situ Hybridization, Male, Platelet-Derived Growth Factor genetics, Proto-Oncogene Proteins c-myc genetics, RNA, Messenger genetics, Receptors, Platelet-Derived Growth Factor metabolism, Arteriosclerosis metabolism, Platelet-Derived Growth Factor metabolism, Proto-Oncogene Proteins c-myc metabolism
- Published
- 1995
- Full Text
- View/download PDF
132. [Expression of PDGF-A and -B in human coronary atherosclerotic lesion: immunohistochemical and in situ hybridization study].
- Author
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Tamamoto T, Toda T, Shimajiri S, Kiyuna M, Shingaki Y, Nakashima Y, and Takei H
- Subjects
- Coronary Artery Disease etiology, Disease Progression, Humans, Immunohistochemistry, In Situ Hybridization, Platelet-Derived Growth Factor physiology, Proto-Oncogene Proteins physiology, Proto-Oncogene Proteins c-sis, Coronary Artery Disease metabolism, Platelet-Derived Growth Factor metabolism, Proto-Oncogene Proteins metabolism, Receptors, Platelet-Derived Growth Factor metabolism
- Abstract
We studied the role of platelet-derived growth factor (PDGF) on the development of atherosclerosis of human coronary arteries of 63 autopsied cases. Smooth muscle cells in fibrocellular intimal thickening lesion showed no significant immunohistochemical reaction of antibodies for PDGF-A or PDGF-B or PDGF-receptor. In atherosclerotic lesions, foam cells derived from macrophages and smooth muscle cells showed intense immunohistochemical reaction with antibodies of PDGF-B and PDGF-receptor, but not with that of PDGF-A. By in situ hybridization, no significant signals of PDGF-A or PDGF-B or PDGF-receptor were demonstrated in proliferating intimal smooth muscle cells in fibrocellular intimal thickening lesions. Uncomplicated atherosclerotic lesions expressed significant amount of m-RNA of c-sis protooncogene and PDGF-B receptor in foam cells. However, no significant signals of PDGF-A were observed in uncomplicated atherosclerotic lesions. These results suggest that foam cells-producing PDGF-B play an important role for the progression of atherosclerotic lesions in human coronary arteries.
- Published
- 1994
133. [An autopsy case of periarteritis nodosa associated with disseminated strongyloidiasis].
- Author
-
Kiyuna M, Toda T, Tamamoto T, Shimajiri S, Shingaki Y, Hokama M, and Ohwan I
- Subjects
- Animals, Female, Humans, Immunocompromised Host, Middle Aged, Polyarteritis Nodosa immunology, Polyarteritis Nodosa pathology, Strongyloidiasis parasitology, Polyarteritis Nodosa complications, Strongyloides stercoralis, Strongyloidiasis etiology
- Abstract
An autopsy case of periarteritis nodosa associated with disseminated strongyloidiasis in a 59-year-old woman is reported. The patient had unknown fever of 38 degrees C and marked impairment of renal function, for which pulse therapy of steroid was performed. Electromyogram showed myogenic pattern, and biopsy of the biceps presented necrotizing angitis of small arteries. Renal biopsy showed marked infiltration of lymphocytes in the stroma, and frequent hyalinosis and crescent formation of the glomeruli. Two months after admission, the patient died of respiratory failure associated with sepsis and disseminated intravascular coagulopathy. Postmortem examination disclosed strongyloides stercolaris and fungi in both lungs with extensive hemorrhage. Terminal ileum and ascending colon had multiple erosions, extensive hemorrhage, numerous strongyloides stercolaris, and frequent necrotizing angitis in the mucosa. Necrotizing angitis was also demonstrated in both kidneys.
- Published
- 1994
134. [Cytofluorometric analysis of DNA content in proliferating cells of coronary arteriosclerotic lesions].
- Author
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Kiyuna M, Toda T, Tamamoto T, Shingaki Y, Shimajiri S, Deguchi S, and Muto Y
- Subjects
- Cell Division, Coronary Artery Disease metabolism, Coronary Vessels chemistry, Female, Flow Cytometry, Humans, Male, Middle Aged, Coronary Artery Disease pathology, Coronary Vessels pathology, DNA analysis
- Abstract
Cytofluorometric determination of DNA content was done on paraffin-embedded tissues of 19 cases of coronary arteriosclerotic lesions including fibrocellular intimal thickening lesions (FT) or atherosclerosis (AS). DNA distribution pattern of medial smooth muscle cells of coronary arteries with FT and AS was all diploid. The average proliferative index (PI) of both medial smooth muscle cells of coronary arteries with FT and AS was 4.8 +/- 0.6. DNA distribution patterns of intimal cells of coronary arteries with FT and AS were also diploid. The average PI of intimal cells of coronary arteries with FT and AS was 8.4 +/- 1.0 and 9.1 +/- 0.7, respectively. These results suggest that intimal cellular proliferation plays an important role in the development of atherosclerosis.
- Published
- 1992
135. [Teenage mothers on isolated islands of the Okinawa Prefecture].
- Author
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Shimajiri S, Miyagi A, Takenaka S, Nakada Y, and Karama H
- Subjects
- Adult, Female, Humans, Infant, Infant, Newborn, Japan, Adolescent, Postnatal Care, Rural Health
- Published
- 1983
136. [Nursing care of the patient in multiple organ failure caused by multiple trauma].
- Author
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Sakugawa S, Hirae K, Nakasone R, Iha N, and Shimajiri S
- Subjects
- Adult, Aged, Female, Humans, Male, Wounds and Injuries complications, Critical Care, Multiple Organ Failure physiopathology, Wounds and Injuries nursing
- Published
- 1984
137. [A survey on the attitude toward breast feeding at obstetrical facilities].
- Author
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Nakamura M, Shimajiri S, Ishiki K, and Miyashiro A
- Subjects
- Female, Humans, Infant, Infant, Newborn, Obstetrics and Gynecology Department, Hospital, Attitude of Health Personnel, Breast Feeding
- Published
- 1987
138. [Instructions and classroom training in obstetrical nursing].
- Author
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Nakamura M, Shimajiri S, and Ishiki K
- Subjects
- Humans, Obstetric Nursing education, Teaching methods
- Published
- 1987
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