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533 results on '"Shi Jin-Song"'

101. Recent progress of W18O49nanowires for energy conversion and storage

Author

Yan, Nan-Fu, Cui, Hong-Min, Shi, Jin-Song, You, Sheng-Yong, and Liu, Sheng

Abstract

W18O49nanowires (W18O49NWs) with unique one-dimension structures and excellent electron/ions transport properties have attracted increasing attention in academia and industry because of their potential applications in many energy-related devices. In the past decades, many research articles related to W18O49have been published, but there are insufficient review articles focusing on W18O49NWs. In this review, we present the crystal structure of W18O49and briefly introduce the synthesis methods and growth mechanism of W18O49NWs. Moreover, their applications in energy conversion and storage devices are summarized. Finally, the current challenges and opportunities for applying W18O49NWs are provided. We hope this review can promote the development of W18O49NWs in energy conversion, storage, and other promising applications.

Published
2023
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110. Biocatalysis

Author

Gong, Jin-Song, primary, Shi, Jin-Song, additional, and Xu, Zheng-Hong, additional

Published
2016
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112. Additional file 1 of High-efficiency secretory expression and characterization of the recombinant type III human-like collagen in Pichia pastoris

Author

Xiang, Zhi-Xiang, Gong, Jin-Song, Shi, Jin-Hao, Liu, Chun-Fang, Li, Heng, Su, Chang, Jiang, Min, Xu, Zheng-Hong, and Shi, Jin-Song

Abstract

Additional file 1: Table S1. List of primers involved in this work. Table S2. List of primers involved in RT-PCR. Fig. S1. SDS-PAGE analysis of 2# recombinant strain. Lanes 1-3 were the blank control (GS115/pPIC9K), lanes 4-6 were the 2# recombinant strain (GS115/pPIC9K-col), and lane M was standard molecular weights. Fig. S2. Target gene copy number of double-driving recombinant strain. Fig. S3. N-terminal sequencing results of the target protein. Fig. S4. C-terminal sequencing results of the target protein. Fig. S5. Western blotting analysis of the native hlCOLIII (after Native-PAGE). Lane 1 was the hlCOLIII, and lane M was standard molecular weights.

Published
2022
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122. Characterization of Cinnamomum kanehiraeExtract-Stimulated Triterpenoids Synthesis in Submerged Fermentation of Antrodia camphoratavia Untargeted Metabolomics

Author

Luo, Zhi-Shan, Lu, Zhen-Ming, Hu, Miao-Miao, Li, Xin-Yang, Xu, Guo-Qiang, Gong, Jin-Song, Shi, Jin-Song, and Xu, Zheng-Hong

Abstract

The underlying mechanisms of Cinnamomum kanehirae-stimulated growth and metabolism of Antrodia camphorataremain unknown. Herein, we first observed that the methanol extract of C. kanehiraetrunk (MECK) (2 g/L) showed a potent stimulatory effect on A. camphoratatriterpenoids production (115.6 mg/L). Second, MECK treatment considerably increased the category and abundance of many secondary metabolites in the mycelia. We identified 93 terpenoids (8 newly formed and 49 upregulated) in the MECK-treated mycelia, wherein 21 terpenoids were the same as those in the fruiting bodies. Third, 42 out of the 93 terpenoids were annotated in the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, mainly involving monoterpenoids and diterpenoids syntheses. Finally, 27 monoterpenes and 16 sesquiterpenes were detected in the MECK, and the two terpenoids with the highest abundance (linalool and α-pinene) were selected for verification and found to considerably increase the terpenoids production of A. camphorataand demonstrate the regulation of mRNA expression levels of nine key genes in the mevalonate pathway via RT-qPCR. This study is beneficial for elucidating the terpenoids synthesis mechanism in A. camphorata.

Published
2023
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123. The Cultural Landscape of the Chinese Bronze Age

Author

Shi Jin-song

Subjects
Archeology, History, Bronze Age, Cultural landscape, Ethnology
Abstract

The Chinese Bronze Age culture from the 19th to the 1st century BC can be divided into four zones: the Central Plain in the middle and the lower Yellow River region; the Northern zone along the Great Wall; the Southern zone, south to the middle and the lower Yangzi River; the Southwestern zone covering the upper Yangzi River. In each zone, bronzes are not only different in types and styles but also function differently responding to the processes of the early state formation and social development. More precisely, in the first zone, there are mainly ritual bronzes, serving as symbols of the social and political hierarchy. In the second zone, there are mainly utilitarian items such as weapons, tools, bronzes of everyday use, ornaments, as well as horse-chariot fittings. Bronze musical instruments characterise the third zone. And bronzes of the fourth zone are realistic or symbolic in style, depicting scenes of social life. Though having different cultural origins and characteristics, the four zones communicate with and influence each other continuously, creating a dynamic cultural landscape of the Chinese Bronze Age.

Published
2019
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124. Desulfovibrio fairfieldensis -Derived Outer Membrane Vesicles Damage Epithelial Barrier and Induce Inflammation and Pyroptosis in Macrophages.

Author

Nie, Yawen, Xie, Xiao-Qian, Zhou, Lingxi, Guan, Qijie, Ren, Yilin, Mao, Yong, Shi, Jin-Song, Xu, Zheng-Hong, and Geng, Yan

Subjects
EXTRACELLULAR vesicles, TIGHT junctions, PYROPTOSIS, TUMOR necrosis factors, PARTICLE size determination, EPITHELIAL cells
Abstract

Sulfate-reducing bacteria Desulfovibrio fairfieldensis is an opportunistic pathogen that widely exists in the human intestine and can cause severe infectious diseases. However, the mechanisms contributing to its pathogenesis remain of great interest. In this study, we aim to investigate the outer membrane vesicles (OMVs) secreted by D. fairfieldensis and their pathogenic effect. The OMVs separated by ultracentrifugation were spherical and displayed a characteristic bilayer lipid structure observed by transmission electron microscopy, with an average hydrodynamic diameter of 75 nm measurement using the particle size analyzer. We identified 1496 and 916 proteins from D. fairfieldensis and its OMVs using label-free non-target quantitative proteomics, respectively. The 560 co-expressed proteins could participate in bacterial life activities by function prediction. The translocation protein TolB, which participates in OMVs biogenesis and transporting toxins was highly expressed in OMVs. The OMVs inhibited the expression of tight junction proteins OCCLUDIN and ZO-1 in human colonic epithelial cells (Caco-2). The OMVs decreased the cell viability of monocyte macrophages (THP-1-Mφ) and activated various inflammatory factors secretion, including interferon-γ (IFN-γ), tumor necrosis factor (TNF-α), and many interleukins. Further, we found the OMVs induced the expression of cleaved-gasdermin D, caspase-1, and c-IL-1β and caused pyroptosis in THP-1-Mφ cells. Taken together, these data reveal that the D. fairfieldensis OMVs can damage the intestinal epithelial barrier and activate intrinsic inflammation. [ABSTRACT FROM AUTHOR]

Published
2023
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131. Restricted intake of sulfur-containing amino acids reversed the hepatic injury induced by excess Desulfovibriothrough gut–liver axis

Author

Zhou, Lingxi, Lu, Gexue, Nie, Yawen, Ren, Yilin, Shi, Jin-Song, Xue, Yuzheng, Xu, Zheng-Hong, and Geng, Yan

Abstract

ABSTRACTDiet is a key player in gut–liver axis. However, the effect of different dietary patterns on gut microbiota and liver functions remains unclear. Here, we used rodent standard chow and purified diet to mimic two common human dietary patterns: grain and plant-based diet and refined-food-based diet, respectively and explored their impacts on gut microbiota and liver. Gut microbiota experienced a great shift with notable increase in Desulfovibrio, gut bile acid (BA) levels elevated significantly, and liver inflammation was observed in mice fed with the purified diet. Liver inflammation and elevated gut BA levels also occurred in mice fed with the chow diet after receiving Desulfovibrio desulfuricansATCC 29,577 (DSV). Restriction of sulfur-containing amino acids (SAAs) prevented liver injury mainly through higher hepatic antioxidant and detoxifying ability and reversed the elevated BA levels due to excess Desulfovibrio. Ex vivofermentation of human fecal microbiota with primary BAs demonstrated that DSV enhanced production of secondary BAs. Higher concentration of both primary and secondary BAs were found in the gut of germ-free mice after receiving DSV. In conclusion, Restriction of SAAs in diet may become an effective dietary intervention to prevent liver injury associated with excess Desulfovibrioin the gut.

Published
2024
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132. Coenzyme Q0 induces apoptosis, senescence and trans-differentiation of activated hepatic stellate cells

Author

Xu, Xin-Yi, Geng, Yan, Xu, Hong-Yu, Shi, Jin-Song, and Xu, Zheng-Hong

Abstract

Figure S1: Pathway functional enrichment of Coenzyme Q0 (7µM) vs control group. Bubble diagram showed KEGG pathway enrichment analysis. X-axis is enrichment ratio [Term Candidate Gene Number/Term Gene Number ], Y-axis is KEGG Pathway, the size of bubbles represents the number of genes annotated to KEGG Pathway, the color represents enrichment Q value, and the darker the color represents Q value, the smaller the value. Figure S2: Enrichment of DEGs in GO compared with the 7µM coenzyme Q0 treatment group. Gene ontology is divided into three major functional categories: molecular function (molecular function), cellular component (cellular component) and biological process (biological process). Perform functional classification based on the results of differential genetic testing. Under each major category, there are sub-categories of respective levels. Plot the GO annotation classification results of differential genes. The X axis represents the number of genes annotated into the GO cylinder, and the Y axis represents GO functional classification. Table S1: The expression levels of differentially expressed genes identified in three comparison groups ( CTL, 7μM CoQ0 treatment, 14μM CoQ0 treatment). Figure S1: Pathway functional enrichment of Coenzyme Q0 (7µM) vs control group. Bubble diagram showed KEGG pathway enrichment analysis. X-axis is enrichment ratio [Term Candidate Gene Number/Term Gene Number ], Y-axis is KEGG Pathway, the size of bubbles represents the number of genes annotated to KEGG Pathway, the color represents enrichment Q value, and the darker the color represents Q value, the smaller the value. Figure S2: Enrichment of DEGs in GO compared with the 7µM coenzyme Q0 treatment group. Gene ontology is divided into three major functional categories: molecular function (molecular function), cellular component (cellular component) and biological process (biological process). Perform functional classification based on the results of differential genetic testing. Under each major category, there are sub-categories of respective levels. Plot the GO annotation classification results of differential genes. The X axis represents the number of genes annotated into the GO cylinder, and the Y axis represents GO functional classification. Table S1: The expression levels of differentially expressed genes identified in three comparison groups ( CTL, 7μM CoQ0 treatment, 14μM CoQ0 treatment).

Published
2020
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140. The tale of a versatile enzyme: Molecular insights into keratinase for its industrial dissemination

Author

Su, Chang, Gong, Jin-Song, Qin, Jiufu, Li, Heng, Li, Hui, Xu, Zheng-Hong, Shi, Jin-Song, Su, Chang, Gong, Jin-Song, Qin, Jiufu, Li, Heng, Li, Hui, Xu, Zheng-Hong, and Shi, Jin-Song

Abstract

Keratinases are unique among proteolytic enzymes for their ability to degrade recalcitrant insoluble proteins, and they are of critical importance in keratin waste management. Over the past few decades, researchers have focused on discovering keratinase producers, as well as producing and characterizing keratinases. The application potential of keratinases has been investigated in the feed, fertilizer, leathering, detergent, cosmetic, and medical industries. However, the commercial availability of keratinases is still limited due to poor productivity and properties, such as thermostability, storage stability and resistance to organic reagents. Advances in molecular biotechnology have provided powerful tools for enhancing the production and functional properties of keratinase. This critical review systematically summarizes the application potential of keratinase, and in particular certain newly discovered catalytic capabilities. Furthermore, we provide comprehensive insight into mechanistic and molecular aspects of keratinases including analysis of gene sequences and protein structures. In addition, development and current advances in protein engineering of keratinases are summarized and discussed, revealing that the engineering of protein domains such as signal peptides and pro-peptides has become an important strategy to increase production of keratinases. Finally, prospects for further development are also proposed, indicating that advanced protein engineering technologies will lead to improved and additional commercial keratinases for various industrial applications.

Published
2020

146. Effects of Cu Doping on the Microstructure and Electrical Properties of Sr3YCo4O10.5+δ Polycrystalline

Author

Shi Jin Song, Bin Zhang, Guo Fang Li, Xiao Li Du, and Lan Yu

Subjects
Crystallography, Materials science, Electrical resistivity and conductivity, Scattering, Doping, General Engineering, Analytical chemistry, Sintering, Crystal structure, Crystallite, Microstructure, Porosity
Abstract

Polycrystalline samples of Sr3YCo4-xCuxO10.5+δ (x=0, 0.2, 0.4, 0.6) have been prepared by solid state reaction method. Crystal structure has been checked by X-ray diffraction (XRD) and the results indicate that Sr3YCo4-xCuxO10.5+δ (x=0, 0.2, 0.4) with single phase have been synthesized in air, and the lattice parameters of Sr3YCo4-xCuxO10.5+δ (x=0, 0.2, 0.4) increase with increasing Cu content. Microstructure analysis demonstrates that the grain sizes enlarge and the number of pores decreases with increasing Cu content. The change of porous structure with different Cu contents is discussed by the liquid sintering theory. The temperature dependence of resistivity (ρ-T) curves of Sr3YCo4-xCuxO10.5+δ show nonmetallic behavior. And the electrical resistivity decreases successively with increasing Cu content, which is caused by the porous structure scattering the carriers as well as the increase of hole carriers.

Published
2014
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150. Systematic engineering for efficient production of nicotinamide mononucleotide from d-xylose and nicotinamide in Escherichia coli

Author

Su, Chang, Cheng, Lin, Gong, Jin-Song, Li, Heng, Xu, Zheng-Hong, and Shi, Jin-Song

Abstract

β-Nicotinamide mononucleotide (NMN) is a direct precursor of the important coenzyme NAD+in the human body and has broad application prospects in healthcare. This study engineered an Escherichia coliBL21 (DE3) strain to efficiently biosynthesize NMN from nicotinamide (NAM) and xylose. Firstly, the nicotinamide phosphoribosyltransferase (Nampt) from Chitinophaga pinensiswas selected and heterologous expressed to construct the NAD+ remediation pathway for NMN synthesis from nicotinamide (NAM) and in vivoprecursor phosphoribosyl pyrophosphate (PRPP). Then, the supply of PRPP was enhanced by constructing the metabolic flux from d-xylose. To facilitate NMN accumulation, the branch pathways, including PRPP competitive pathway, carbon flow competitive pathway, and NMN downstream metabolic decomposition pathway were fine-tuned using CRISPR/Cas9 editing tools. Combined with process optimization of whole-cell biocatalytic reaction, a NMN titre of 497.5 mg·L−1was obtained. Finally, the scale-up culture was carried out on a 5 L fermenter, and the yield reached 760.2 mg·L−1. This work achieved green biosynthesis of NMN using xylose as substrate and enhanced the productivity by systematic engineering strategies, presenting more possibilities for the synthesis of NMN from a wide range of monosaccharides.

Published
2024
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