Search

Your search keyword '"Shen, Ren-Fang"' showing total 453 results
Start Over Author "Shen, Ren-Fang"
453 results on '"Shen, Ren-Fang"'

118. Preferential Distribution of Boron to Developing Tissues Is Mediated by the Intrinsic Protein OsNIP31[OPEN]

Author

Shao, Ji Feng, Yamaji, Naoki, Liu, Xin Wei, Yokosho, Kengo, Shen, Ren Fang, and Ma, Jian Feng

Subjects
inorganic chemicals, Plant Leaves, Xylem, Meristem, food and beverages, Membrane Proteins, Biological Transport, Oryza, Articles, Boron, Plant Proteins
Abstract

Boron is especially required for the growth of meristem and reproductive organs, but the molecular mechanisms underlying the preferential distribution of B to these developing tissues are poorly understood. Here, we show evidence that a member of nodulin 26-like intrinsic protein (NIP), OsNIP3;1, is involved in this preferential distribution in rice (

Published
2017

122. Putrescine Alleviates Iron Deficiency via NO-Dependent Reutilization of Root Cell-Wall Fe in Arabidopsis1[OPEN]

Author

Zhu, Xiao Fang, Wang, Bin, Song, Wen Feng, Zheng, Shao Jian, and Shen, Ren Fang

Subjects
FMN Reductase, Arabidopsis Proteins, Carboxy-Lyases, Iron, Arabidopsis, Articles, Nitric Oxide, Plant Roots, Cell Wall, Gene Expression Regulation, Plant, Polysaccharides, Mutation, Basic Helix-Loop-Helix Transcription Factors, Putrescine, Cation Transport Proteins
Abstract

Plants challenged with abiotic stress show enhanced polyamines levels. Here, we show that the polyamine putrescine (Put) plays an important role to alleviate Fe deficiency. The adc2-1 mutant, which is defective in Put biosynthesis, was hypersensitive to Fe deficiency compared with wild type (Col-1 of Arabidopsis [Arabidopsis thaliana]). Exogenous Put decreased the Fe bound to root cell wall, especially to hemicellulose, and increased root and shoot soluble Fe content, thus alleviating the Fe deficiency-induced chlorosis. Intriguingly, exogenous Put induced the accumulation of nitric oxide (NO) under both Fe-sufficient (+Fe) and Fe-deficient (-Fe) conditions, although the ferric-chelate reductase (FCR) activity and the expression of genes related to Fe uptake were induced only under -Fe treatment. The alleviation of Fe deficiency by Put was diminished in the hemicellulose-level decreased mutant-xth31 and in the noa1 and nia1nia2 mutants, in which the endogenous NO levels are reduced, indicating that both NO and hemicellulose are involved in Put-mediated alleviation of Fe deficiency. However, the FCR activity and the expression of genes related to Fe uptake were still up-regulated under -Fe+Put treatment compared with -Fe treatment in xth31, and Put-induced cell wall Fe remobilization was abolished in noa1 and nia1nia2, indicating that Put-regulated cell wall Fe reutilization is dependent on NO. From our results, we conclude that Put is involved in the remobilization of Fe from root cell wall hemicellulose in a process dependent on NO accumulation under Fe-deficient condition in Arabidopsis.

Published
2015

125. Boron Supply Enhances Aluminum Tolerance in Root Border Cells of Pea (Pisum sativum) by Interacting with Cell Wall Pectins

Author

Li, Xue Wen, primary, Liu, Jia You, additional, Fang, Jing, additional, Tao, Lin, additional, Shen, Ren Fang, additional, Li, Ya Lin, additional, Xiao, Hong Dong, additional, Feng, Ying Ming, additional, Wen, Hai Xiang, additional, Guan, Jia Hua, additional, Wu, Li Shu, additional, He, Yong Ming, additional, Goldbach, Heiner E., additional, and Yu, Min, additional

Published
2017
Full Text
View/download PDF

128. Case of a stronger capability of maize seedlings to use ammonium being responsible for the higher 15N recovery efficiency of ammonium compared with nitrate.

Author

Zhang, Hao Qing, Zhao, Xue Qiang, Chen, Yi Ling, Zhang, Ling Yu, and Shen, Ren Fang

Subjects
AMMONIUM nitrate, CORN, SEEDLINGS, CORN growth, ROOT growth, AMMONIUM
Abstract

Aims: Matching a crop's nitrogen (N) preference with the specific form of N is critical to enhance N recovery efficiency (NRE). Many studies show that maize (Zea mays L.) has a preference for NH4+ uptake over NO3−, but the contribution of this preference to NRE is unclear. This study evaluated the utilization of ammonium (NH4+) and nitrate (NO3−) by maize and its relationship with NRE. Methods: Maize (cv. 'Zhengdan 958') seedlings were grown in a soil culture experiment with 15NH4+ or 15NO3− applied to sterilized or non-sterilized soils, and in a hydroponic experiment supplied with NH4+ or NO3−. We recorded maize root and shoot growth and N uptake, and the distribution of 15N-labeled N in the soil–plant system. Results: Ammonium application enhanced maize root and shoot growth and N uptake compared with those of NO3−-treated plants in both culture systems. This enhancement by NH4+ was further promoted in sterilized soil. More applied N remained in soil supplied with NO3− than with NH4+ after seedling harvest. The NRE of NH4+ in maize was significantly higher than that of NO3−, while a similar proportion of applied N was lost with NH4+ or NO3−supply. Conclusions: Ammonium supply enhances maize growth and NRE compared with NO3− under the present experimental condition, which may be attributable to the superior capability of maize to utilize NH4+. [ABSTRACT FROM AUTHOR]

Published
2019
Full Text
View/download PDF

129. Towards sustainable use of acidic soils: Deciphering aluminum-resistant mechanisms in plants

Author

Zhu, Xiao Fang and Shen, Ren Fang

Abstract

The widespread occurrence of acidic soils presents a major challenge for agriculture, as it hampers productivity via a combination of mineral toxicity, nutrient deficiency, and poor water uptake. Conventional remediation methods, such as amending the soil with lime, magnesium, or calcium, are expensive and not environmentally friendly. The most effective method to mitigate soil acidity is the cultivation of acid-tolerant cultivars. The ability of plants to tolerate acidic soils varies significantly, and a key factor influencing this tolerance is aluminum (Al) toxicity. Therefore, understanding the physiological, molecular, and genetic underpinnings of Al tolerance is essential for the successful breeding of acid-tolerant crops. Different tolerance mechanisms are regulated by various genes and quantitative trait loci in various plant species, and molecular markers have been developed to facilitate gene cloning and to support marker-assisted selection for breeding Al-tolerant cultivars. This study provides a comprehensive review of the current developments in understanding the physiological and molecular mechanisms underlying Al resistance. Through the application of genome-wide association methods, it is expected that new Al-resistant genes can be identified and utilized to cultivate Al-resistant varieties through intercrossing, backcrossing, and molecular marker-assisted selection, promoting the sustainable use of acidic soils.

Published
2024
Full Text
View/download PDF

139. Complete mitochondrial genome of the aluminum-tolerant fungus Rhodotorula taiwanensis RS1 and comparative analysis of Basidiomycota mitochondrial genomes

Author

Zhao, Xue Qiang, Aizawa, Tomoko, Schneider, Jessica, Wang, Chao, Shen, Ren Fang, and Sunairi, Michio

Subjects
Base Composition, Annotation, Basidiomycota, Rhodotorula, comparative genomics, Sequence Analysis, DNA, basidiomycetous yeasts, DNA, Mitochondrial, Introns, Open Reading Frames, RNA, Ribosomal, Genome, Mitochondrial, Genome, Fungal, Codon, DNA, Fungal, Phylogeny, Original Research, Aluminum
Abstract

The complete mitochondrial genome of Rhodotorula taiwanensis RS1, an aluminum-tolerant Basidiomycota fungus, was determined and compared with the known mitochondrial genomes of 12 Basidiomycota species. The mitochondrial genome of R. taiwanensis RS1 is a circular DNA molecule of 40,392 bp and encodes the typical 15 mitochondrial proteins, 23 tRNAs, and small and large rRNAs as well as 10 intronic open reading frames. These genes are apparently transcribed in two directions and do not show syntenies in gene order with other investigated Basidiomycota species. The average G+C content (41%) of the mitochondrial genome of R. taiwanensis RS1 is the highest among the Basidiomycota species. Two introns were detected in the sequence of the atp9 gene of R. taiwanensis RS1, but not in that of other Basidiomycota species. Rhodotorula taiwanensis is the first species of the genus Rhodotorula whose full mitochondrial genome has been sequenced; and the data presented here supply valuable information for understanding the evolution of fungal mitochondrial genomes and researching the mechanism of aluminum tolerance in microorganisms.

Published
2013

140. Diazotroph abundance and community composition in an acidic soil in response to aluminum-tolerant and aluminum-sensitive maize (<italic>Zea mays</italic> L.) cultivars under two nitrogen fertilizer forms.

Author

Wang, Chao, Zheng, Man Man, Hu, An Yong, Zhu, Chun Quan, and Shen, Ren Fang

Subjects
PLANT growth, ALUMINUM content of plants, NITROGEN content of plants, SOIL acidity, ACID soils
Abstract

Aims: In acidic soil, plant aluminum (Al) tolerance and nitrogen (N) fertilizer form play the important roles in influencing plant growth. Diazotrophs as plant growth promoting rhizobacteria can contribute to plant available N, but the response to plant growth differences resulting from plant Al tolerance or N fertilizer form in acidic soil is poorly understood. Here, we investigated this response.Methods: Three maize cultivars with different Al-tolerance levels were grown in an acidic soil that was added with nitrate (NO3−-N) or ammonium (NH4+-N) fertilizers. After 42 days, maize biomasses and root morphologies as well as the physicochemical properties of bulk and rhizosphere soils were determined. Based on nifH gene, soil diazotroph abundance was determined by quantitative RT-PCR and community composition was assayed using high-throughput sequencing.Results: For the same maize cultivar, application of NO3−-N fertilizer relative to NH4+-N fertilizer increased maize biomass, root length, root surface area, and rhizosphere pH, but not significantly affect rhizosphere nifH copy number and community composition. The nifH copy numbers did not show significant differences among rhizosphere samples of three maize cultivars, but community composition was obviously different between Al-tolerant and Al-sensitive cultivars.Conclusions: In acidic soil, maize cultivars depending on Al tolerance altered rhizosphere diazotrophic community composition, but this effect seemed to be not susceptible to N fertilizer forms. [ABSTRACT FROM AUTHOR]

Published
2018
Full Text
View/download PDF

147. PARVUS affects aluminium sensitivity by modulating the structure of glucuronoxylan in Arabidopsis thaliana.

Author

Zhu, Xiao Fang, Wan, Jiang Xue, Wu, Qi, Zhao, Xu Sheng, Zheng, Shao Jian, and Shen, Ren Fang

Subjects
HEMICELLULOSE, PLANT cell walls, ALUMINUM, ARABIDOPSIS thaliana, SECONDARY metabolism
Abstract

Glucuronoxylan (GX), an important component of hemicellulose in the cell wall, appears to affect aluminium (Al) sensitivity in plants. To investigate the role of GX in cell-wall-localized xylan, we examined the Arabidopsis thaliana parvus mutant in detail. This mutant lacks α-D-glucuronic acid (GlcA) side chains in GX and has greater resistance to Al stress than wild-type (WT) plants. The parvus mutant accumulated lower levels of Al in its roots and cell walls than WT despite having cell wall pectin content and pectin methylesterase (PME) activity similar to those of WT. Our results suggest that the altered properties of hemicellulose in the mutant contribute to its decreased Al accumulation. Although we observed almost no differences in hemicellulose content between parvus and WT under control conditions, less Al was retained in parvus hemicellulose than in WT. This observation is consistent with the finding that GlcA substitutions in WT GX, but not mutant GX, were increased under Al stress. Taken together, these results suggest that the modulation of GlcA levels in GX affects Al resistance by influencing the Al binding capacity of the root cell wall in Arabidopsis. [ABSTRACT FROM AUTHOR]

Published
2017
Full Text
View/download PDF

148. Immobilization of Aluminum with Phosphorus in Roots Is Associated with High Aluminum Resistance in Buckwheat1

Author

Zheng, Shao Jian, Yang, Jian Li, He, Yun Feng, Yu, Xue Hui, Zhang, Lei, You, Jiang Feng, Shen, Ren Fang, and Matsumoto, Hideaki

Subjects
Kinetics, Dose-Response Relationship, Drug, Oxalic Acid, food and beverages, Phosphorus, Plant Roots, Immunity, Innate, Research Article, Aluminum, Fagopyrum
Abstract

Oxalic acid secretion from roots is considered to be an important mechanism for aluminum (Al) resistance in buckwheat (Fygopyrum esculentum Moench). Nonetheless, only a single Al-resistant buckwheat cultivar was used to investigate the significance of oxalic acid in detoxifying Al. In this study, we investigated two buckwheat cultivars, Jiangxi (Al resistant) and Shanxi (Al sensitive), which showed significant variation in their resistance to Al stress. In the presence of 0 to 100 microM Al, the inhibition of root elongation was greater in Shanxi than that in Jiangxi, and the Al content of root apices (0-10 mm) was much lower in Jiangxi. However, the dependence of oxalic acid secretion on external Al concentration and the time course for secretion were similar in both cultivars. Furthermore, the variation in Al-induced oxalic acid efflux along the root was similar, showing a 10-fold greater efflux from the apical 0- to 5-mm region than from the 5- to 10-mm region. These results suggest that both Shanxi and Jiangxi possess an equal capacity for Al-dependent oxalic acid secretion. Another two potential Al resistance mechanisms, i.e. Al-induced alkalinization of rhizosphere pH and root inorganic phosphate release, were also not involved in their differential Al resistance. However, after longer treatments in Al (10 d), the concentrations of phosphorus and Al in the roots of the Al-resistant cultivar Jiangxi were significantly higher than those in Shanxi. Furthermore, more Al was localized in the cell walls of the resistant cultivar. All these results suggest that while Al-dependent oxalic acid secretion might contribute to the overall high resistance to Al stress of buckwheat, this response cannot explain the variation in tolerance between these two cultivars. We present evidence suggesting the greater Al resistance in buckwheat is further related to the immobilization and detoxification of Al by phosphorus in the root tissues.

Published
2005

149. A potential contribution of the less negatively charged cell wall to the high aluminum tolerance of Rhodotorula taiwanensis RS1.

Author

Hu, Zhen Min, Zhao, Xue Qiang, Bao, Xue Min, Wang, Chao, Wang, Wei, Zheng, Lu, Lan, Ping, and Shen, Ren Fang

Abstract

Rhodotorula taiwanensis RS1 (Rt) is a high-aluminum (Al)-tolerant yeast that can survive Al at concentrations up to 200 mM. In this study, we compared Rt with an Al-sensitive congeneric strain, R. mucilaginosa AKU 4812 (Rm) and Al sensitive mutant 1 ( alsm1) of Rt, to explore the Al tolerance mechanisms of Rt. The growth of Rm was completely inhibited by 1 mM Al, but that of Rt was not inhibited until Al concentration was more than 70 mM. The growth of alsm1 was inhibited much more by 70 mM and 100 mM Al than that of Rt. Compared with Rm cells, Rt cells accumulated less Al in the cell wall and cytoplasm. A time-course analysis showed that Al was absorbed by Rm cells much more rapidly than by Rt cells when exposed to the same Al concentration. Meanwhile, the Al content of alsm1 was higher than that of Rt. Although the cell wall of Rt was thicker than that of alsm1 and Rm under control and 0.1 mM Al, that of Rt was thinner than that of alsm1 under 70 mM Al despite that their cell walls were thickened. The alcian blue adsorption was lower and cell wall zeta-potential was higher in Rt and alsm1 than in Rm, indicating a less negative charge of cell wall of Rt and alsm1 than that of Rm. Taken together, the less negatively charged cell wall of Rt may restrict the adsorption of cationic Al in cells, potentially contributing to its high Al tolerance. Copyright © 2016 John Wiley & Sons, Ltd. [ABSTRACT FROM AUTHOR]

Published
2016
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results