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576 results on '"Shaw, J. M."'

102. 5213

Author

Seidman, T. I., Altgeld, J. P., and Shaw, J. M.

Published
1965
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107. Greening's challenge: Can the new Development Secretary convince Cameron to take down the aid 'ringfence'?

Author

Shaw, J. M.

Subjects
Secretaries of state -- Beliefs, opinions and attitudes, Fiscal policy, General interest, News, opinion and commentary, Political science
Abstract

At first glance, it looked like very good news when David Cameron appointed Justine Greening as Secretary of State for International Development in his September 2012 reshuffle. Greening is an [...]

Published
2013

122. Military Operations in the Cold: Effects on Anaerobic-Muscular Performance and Select Blood Indices

Author

NAVAL HEALTH RESEARCH CENTER SAN DIEGO CA, Hackney, A. C., Shaw, J. M., Hodgdon, J. A., Coyne, J. T., Kelleher, D. L., NAVAL HEALTH RESEARCH CENTER SAN DIEGO CA, Hackney, A. C., Shaw, J. M., Hodgdon, J. A., Coyne, J. T., and Kelleher, D. L.

Abstract

Many reports have established the existence of negative physiological and psychological effects developing in humans due to prolonged cold weather exposure. Dehydration, soft tissue injuries, increased energy expenditure, elevated fatigue, depression, and impaired cognition are a few of the problems associated with exposure to this environment. Furthermore, it is well established that during extended military field maneuvers extreme physical demands are placed on personnel. The combined effects of an adverse environment and high physical workloads can significantly degrade human performance. This latter issue, decrement of human performance, is a key concern of military biomedical researchers because of the potential impact to the success of military operations. (js)

Published
1990

147. Comparison of slow- and rapid-cooling protocols for early-cleavage-stage Macaca fascicularis embryos.

Author

Curnow, E. C., Kuleshova, L. L., Shaw, J. M., and Hayes, E. S.

Subjects
KRA, FERTILIZATION in vitro, CRYOPRESERVATION of organs, tissues, etc., EMBRYOS, REPRODUCTION, PRIMATES
Abstract

Cryostorage of nonhuman primate embryos by time-consuming slow-cooling methods is often limited to early cleavage stages. Effective rapid-cooling methods have been developed for many species and represent valuable tools for laboratory- and field-based studies of nonhuman primate reproductive biology. However, few rapid-cooling protocols have been applied to nonhuman primate embryos in terms of comparing various developmental stages. Here we compare slow cooling vs. two- and three-step rapid cooling of two-, four-, and eight-cell Macaca fascicularis (Mf ) embryos. Rapid cooling was conducted in open pulled straws (OPS) using cooling solutions containing reduced quantities of ethylene glycol (EG) and supplemented with either of two high-molecular-weight polymers, ficoll and dextran. The survival of the slow-cooled embryos, but not the rapid-cooled embryos, was independent of embryonic stage at cryostorage. Slow cooling was associated with greater cell survival (82%) post thaw compared to warming following rapid cooling (18–29%). Slow cooling resulted in a high proportion of embryo survival (18/20; 90%) and cleavage (15/18; 83%) post thaw. Rapid cooling resulted in significantly reduced percentages of embryo survival (26–32%) and embryo cleavage in culture (29–38%) after warming. Conventional slow cooling was more effective than the rapid-cooling protocols employed in this study for cryopreservation of early-cleavage-stage Mf embryos. Am. J. Primatol. 58:169–174, 2002. © 2002 Wiley-Liss, Inc. [ABSTRACT FROM AUTHOR]

Published
2002
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148. A strategy for rapid cooling of mouse embryos within a double straw to eliminate the risk of contamination during storage in liquid nitrogen.

Author

Kuleshova, L. L. and Shaw, J. M.

Abstract

Double packaging, in which an inner straw containing the specimen is inserted into an outer, larger straw (here termed 'straw-in-straw') to prevent the inner straw from coming into direct contact with liquid nitrogen provides a simple strategy for reducing or eliminating the potential contamination risk associated with storage in liquid nitrogen. This approach has in the past been used in conjunction with cryopreservation by slow cooling, but has not previously been tested for use throughout an entire rapid cooling and warming procedure. This study determined whether keeping the straw containing the embryos inside a second protecting container throughout the cryopreservation and storage protocol would compromise embryo viability. We established that a cryoprotectant containing a high polymer concentration (35% dextran or Ficoll) together with 25% ethylene glycol (as the penetrating cryoprotectant) was highly effective for day 2 and day 3 mouse embryos in both single and double straws. The survival and development of all cryopreserved embryos, as assessed both in vitro and in vivo, was not statistically different to their untreated controls. This established that a protein/serum-free cryoprotectant solution supplemented with polymers could provide complete protection of mouse embryos. It also shows, for the first time, that embryos can be cooled by direct immersion in liquid nitrogen and warmed by direct immersion into a waterbath within a double straw arrangement to reduce the likelihood of contamination. [ABSTRACT FROM AUTHOR]

Published
2000
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149. Highly-ordered nanodots for future data storage system

Author

Shaw, J. M.

Subjects
Information storage and retrieval -- Industry forecasts -- Usage, Nanotechnology -- Usage, Information storage and retrieval systems -- Product development -- Usage, Business, High technology industry
Abstract

Digital data storage systems are estimated to double every year and nanotechnology has been reckoned to be a possible solution for answering tomorrow's storage needs. However, numerous problems arise from [...]

Published
2007

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