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101. Transmission of B.1.617.2 Delta variant between vaccinated healthcare workers

Author

Kemp, Steven, Cheng, Mark TK, Hamilton, William L, Kamelian, Kimia, Chauhan, Himanshu, Dikid, Tanzin, Gogia, Hema, Lall, Hemlata, Ponnusamy, Kalaiarasan, Verma, Kaptan, Dhar, Mahesh Shanker, Singh, Manoj K, Datta, Meena, Soni, Namita, Meena, Namonarayan, Madan, Preeti, Singh, Priyanka, Sharma, Ramesh, Sharma, Rajeev, Kabra, Sandhya, Kumar, Sattender, Kumari, Swati, Sharma, Uma, Chaudhary, Urmila, Sivasubbu, Sridhar, Scaria, Vinod, Wattal, Chand, Oberoi, JK, Raveendran, Reena, Datta, S, Das, Saumitra, Maitra, Arindam, Chinnaswamy, Sreedhar, Biswas, Nidhan Kumar, Parida, Ajay, Raghav, Sunil K, Prasad, Punit, Sarin, Apurva, Mayor, Satyajit, Ramakrishnan, Uma, Palakodeti, Dasaradhi, Seshasayee, Aswin Sai Narain, Thangaraj, K, Bashyam, Murali Dharan, Dalal, Ashwin, Bhat, Manoj, Shouche, Yogesh, Pillai, Ajay, Abraham, Priya, Potdar, Varsha Atul, Cherian, Sarah S, Desai, Anita Sudhir, Pattabiraman, Chitra, Manjunatha, MV, Mani, Reeta S, Udupi, Gautam Arunachal, Nandicoori, Vinay, Tallapaka, Karthik Bharadwaj, Sowpati, Divya Tej, Singh, Sujit, Rakshit, Partha, Agrawal, Anurag, Illingworth, Christopher, Gupta, Ravindra, Hamilton, William L [0000-0002-3330-353X], Apollo - University of Cambridge Repository, Kemp, Steven [0000-0001-7077-6793], and Gupta, Ravindra [0000-0001-9751-1808]

Subjects
Infection Control, SARS-CoV-2, Health Personnel, Vaccination, COVID-19, Humans
Abstract

Breakthrough infections with SARS-CoV-2 Delta variant have been reported in doubly-vaccinated recipients and as re-infections. Studies of viral spread within hospital settings have highlighted the potential for transmission between doubly-vaccinated patients and health care workers and have highlighted the benefits of high-grade respiratory protection for health care workers. However the extent to which vaccination is preventative of viral spread in health care settings is less well studied. Here, we analysed data from 118 vaccinated health care workers (HCW) across two hospitals in India, constructing two probable transmission networks involving six HCWs in Hospital A and eight HCWs in Hospital B from epidemiological and virus genome sequence data, using a suite of computational approaches. A maximum likelihood reconstruction of transmission involving known cases of infection suggests a high probability that doubly vaccinated HCWs transmitted SARS-CoV-2 between each other and highlights potential cases of virus transmission between individuals who had received two doses of vaccine. Our findings show firstly that vaccination may reduce rates of transmission, supporting the need for ongoing infection control measures even in highly vaccinated populations, and secondly we have described a novel approach to identifying transmissions that is scalable and rapid, without the need for an infection control infrastructure.

Published
2022

102. SARS-CoV-2 B.1.617.2 Delta variant replication and immune evasion

Author

Mlcochova, Petra, Kemp, Steven A., Dhar, Mahesh Shanker, Papa, Guido, Meng, Bo, Ferreira, Isabella A. T. M., Datir, Rawlings, Collier, Dami A., Albecka, Anna, Singh, Sujeet, Pandey, Rajesh, Brown, Jonathan, Zhou, Jie, Goonawardane, Niluka, Mishra, Swapnil, Whittaker, Charles, Mellan, Thomas, Marwal, Robin, Datta, Meena, Sengupta, Shantanu, Ponnusamy, Kalaiarasan, Radhakrishnan, Venkatraman Srinivasan, Abdullahi, Adam, Charles, Oscar, Chattopadhyay, Partha, Devi, Priti, Caputo, Daniela, Peacock, Tom, Wattal, Chand, Goel, Neeraj, Satwik, Ambrish, Vaishya, Raju, Agarwal, Meenakshi, Chauhan, Himanshu, Dikid, Tanzin, Gogia, Hema, Lall, Hemlata, Verma, Kaptan, Singh, Manoj K., Soni, Namita, Meena, Namonarayan, Madan, Preeti, Singh, Priyanka, Sharma, Ramesh, Sharma, Rajeev, Kabra, Sandhya, Kumar, Sattender, Kumari, Swati, Sharma, Uma, Chaudhary, Urmila, Sivasubbu, Sridhar, Scaria, Vinod, Oberoi, J. K., Raveendran, Reena, Datta, S., Das, Saumitra, Maitra, Arindam, Chinnaswamy, Sreedhar, Biswas, Nidhan Kumar, Parida, Ajay, Raghav, Sunil K., Prasad, Punit, Sarin, Apurva, Mayor, Satyajit, Ramakrishnan, Uma, Palakodeti, Dasaradhi, Seshasayee, Aswin Sai Narain, Thangaraj, K., Bashyam, Murali Dharan, Dalal, Ashwin, Bhat, Manoj, Shouche, Yogesh, Pillai, Ajay, Abraham, Priya, Potdar, Varsha Atul, Cherian, Sarah S., Desai, Anita Sudhir, Pattabiraman, Chitra, Manjunatha, M. V., Mani, Reeta S., Udupi, Gautam Arunachal, Nandicoori, Vinay, Tallapaka, Karthik Bharadwaj, Sowpati, Divya Tej, Kawabata, Ryoko, Morizako, Nanami, Sadamasu, Kenji, Asakura, Hiroyuki, Nagashima, Mami, Yoshimura, Kazuhisa, Ito, Jumpei, Kimura, Izumi, Uriu, Keiya, Kosugi, Yusuke, Suganami, Mai, Oide, Akiko, Yokoyama, Miyabishara, Chiba, Mika, Saito, Akatsuki, Butlertanaka, Erika P., Tanaka, Yuri L., Ikeda, Terumasa, Motozono, Chihiro, Nasser, Hesham, Shimizu, Ryo, Yuan, Yue, Kitazato, Kazuko, Hasebe, Haruyo, Nakagawa, So, Wu, Jiaqi, Takahashi, Miyoko, Fukuhara, Takasuke, Shimizu, Kenta, Tsushima, Kana, Kubo, Haruko, Shirakawa, Kotaro, Kazuma, Yasuhiro, Nomura, Ryosuke, Horisawa, Yoshihito, Takaori-Kondo, Akifumi, Tokunaga, Kenzo, Ozono, Seiya, Baker, Stephen, Dougan, Gordon, Hess, Christoph, Kingston, Nathalie, Lehner, Paul J., Lyons, Paul A., Matheson, Nicholas J., Owehand, Willem H., Saunders, Caroline, Summers, Charlotte, Thaventhiran, James E. D., Toshner, Mark, Weekes, Michael P., Maxwell, Patrick, Shaw, Ashley, Bucke, Ashlea, Calder, Jo, Canna, Laura, Domingo, Jason, Elmer, Anne, Fuller, Stewart, Harris, Julie, Hewitt, Sarah, Kennet, Jane, Jose, Sherly, Kourampa, Jenny, Meadows, Anne, O'Brien, Criona, Price, Jane, Publico, Cherry, Rastall, Rebecca, Ribeiro, Carla, Rowlands, Jane, Ruffolo, Valentina, Tordesillas, Hugo, Bullman, Ben, Dunmore, Benjamin J., Fawke, Stuart, Graf, Stefan, Hodgson, Josh, Huang, Christopher, Hunter, Kelvin, Jones, Emma, Legchenko, Ekaterina, Matara, Cecilia, Martin, Jennifer, Mescia, Federica, O'Donnell, Ciara, Pointon, Linda, Pond, Nicole, Shih, Joy, Sutcliffe, Rachel, Tilly, Tobias, Treacy, Carmen, Tong, Zhen, Wood, Jennifer, Wylot, Marta, Bergamaschi, Laura, Betancourt, Ariana, Bower, Georgie, Cossetti, Chiara, De Sa, Aloka, Epping, Madeline, Gleadall, Nick, Grenfell, Richard, Hinch, Andrew, Huhn, Oisin, Jackson, Sarah, Jarvis, Isobel, Krishna, Ben, Lewis, Daniel, Marsden, Joe, Nice, Francesca, Okecha, Georgina, Omarjee, Ommar, Perera, Marianne, Potts, Martin, Richoz, Nathan, Romashova, Veronika, Yarkoni, Natalia Savinykh, Sharma, Rahul, Stefanucci, Luca, Stephens, Jonathan, Strezlecki, Mateusz, Turner, Lori, De Bie, Eckart M. D. D., Bunclark, Katherine, Josipovic, Masa, Mackay, Michael, Rossi, Sabrina, Selvan, Mayurun, Spencer, Sarah, Yong, Cissy, Allison, John, Butcher, Helen, Clapham-Riley, Debbie, Dewhurst, Eleanor, Furlong, Anita, Graves, Barbara, Gray, Jennifer, Ivers, Tasmin, Kasanicki, Mary, Le Gresley, Emma, Linger, Rachel, Meloy, Sarah, Muldoon, Francesca, Ovington, Nigel, Papadia, Sofia, Phelan, Isabel, Stark, Hannah, Stirrups, Kathleen E., Townsend, Paul, Walker, Neil, Webster, Jennifer, Scholtes, Ingrid, Hein, Sabine, King, Rebecca, Mavousian, Antranik, Lee, Joo Hyeon, Bassi, Jessica, Silacci-Fegni, Chiara, Saliba, Christian, Pinto, Dora, Irie, Takashi, Yoshida, Isao, Hamilton, William L., Sato, Kei, Bhatt, Samir, Flaxman, Seth, James, Leo C., Corti, Davide, Piccoli, Luca, Barclay, Wendy S., Rakshit, Partha, Agrawal, Anurag, Gupta, Ravindra K., (INSACOG), Indian SARS-CoV-2 Genomics Consortium, Consortium, Genotype to Phenotype Japan (G2P-Japan), Collaboration, CITIID-NIHR BioResource COVID-19, Gupta, Ravindra K [0000-0001-9751-1808], Apollo - University of Cambridge Repository, and Gupta, Ravindra K. [0000-0001-9751-1808]

Subjects
Male, COVID-19 Vaccines, medicine.drug_class, Health Personnel, India, Monoclonal antibody, Virus Replication, Antibodies, Cell Line, Cell Fusion, Immune system, 13/100, medicine, Humans, Neutralizing antibody, Antibodies, Neutralizing, Female, Kinetics, SARS-CoV-2, Spike Glycoprotein, Coronavirus, Vaccination, Immune Evasion, Neutralizing, 631/326/596/4130, Syncytium, Multidisciplinary, Cell fusion, biology, article, Vaccine efficacy, 631/250/254, Virology, Spike Glycoprotein, Coronavirus, 13/31, biology.protein, Antibody, Infection
Abstract

The B.1.617.2 (Delta) variant of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was first identified in the state of Maharashtra in late 2020 and spread throughout India, outcompeting pre-existing lineages including B.1.617.1 (Kappa) and B.1.1.7 (Alpha)1. In vitro, B.1.617.2 is sixfold less sensitive to serum neutralizing antibodies from recovered individuals, and eightfold less sensitive to vaccine-elicited antibodies, compared with wild-type Wuhan-1 bearing D614G. Serum neutralizing titres against B.1.617.2 were lower in ChAdOx1 vaccinees than in BNT162b2 vaccinees. B.1.617.2 spike pseudotyped viruses exhibited compromised sensitivity to monoclonal antibodies to the receptor-binding domain and the amino-terminal domain. B.1.617.2 demonstrated higher replication efficiency than B.1.1.7 in both airway organoid and human airway epithelial systems, associated with B.1.617.2 spike being in a predominantly cleaved state compared with B.1.1.7 spike. The B.1.617.2 spike protein was able to mediate highly efficient syncytium formation that was less sensitive to inhibition by neutralizing antibody, compared with that of wild-type spike. We also observed that B.1.617.2 had higher replication and spike-mediated entry than B.1.617.1, potentially explaining the B.1.617.2 dominance. In an analysis of more than 130 SARS-CoV-2-infected health care workers across three centres in India during a period of mixed lineage circulation, we observed reduced ChAdOx1 vaccine effectiveness against B.1.617.2 relative to non-B.1.617.2, with the caveat of possible residual confounding. Compromised vaccine efficacy against the highly fit and immune-evasive B.1.617.2 Delta variant warrants continued infection control measures in the post-vaccination era., A study of SARS-CoV-2 variants examining their transmission, infectivity, and potential resistance to therapies provides insights into the biology of the Delta variant and its role in the global pandemic.

Published
2022
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105. Exploring Full Adder Design Options for 90nm Technology Node: An In-depth Performance Analysis.

Author

Shukla, Shrasti Sanjay, Tripathi, Shivanshu, and Sharma, Uma

Subjects
LOGIC circuits, DESIGN techniques, LOW voltage systems, VERY large scale circuit integration
Abstract

This study presents a comprehensive examination of the various full adder circuit designs, which form the core of many high computational arithmetic logic circuits in low voltage low power VLSI designs. Any researcher's ultimate objective is to increase speed and efficiency while minimizing the circuit's need for space by optimizing its performance. In this paper, the benefits and drawbacks of several design techniques employed in the development of the full adder have been examined. Various state-of-the-art full adders at the 90nm technology node have also been compared and analyzed. [ABSTRACT FROM AUTHOR]

Published
2023

106. USES OF THE FIRST LANGUAGE IN THE ENGLISH AS A FOREIGN/SECOND LANGUAGE CLASSROOM: TEACHERS' VIEWS AND ARGUMENTS.

Author

Luitel, Binod, Poudel, Kamal Kumar, Sharma, Uma Nath, and Karki, Tek Mani

Subjects
LANGUAGE teachers, ENGLISH as a foreign language, ENGLISH teachers, TEACHERS, LANGUAGE transfer (Language learning), ENGLISH language, SECOND language acquisition
Abstract

Drawing on the lived experiences of three community school English teachers in Nepal, this article explores the uses of the learners' first language (L1) in English as a foreign/second language (ESL) classes. As the data, we collected the written lived-experience descriptions from the teachers, observed their classes, and interviewed them. We analyzed the data thematically and interpreted them contextually. As the study found, the teachers have their lived experiences of using the L1 for its cognitive roles - namely, for checking learners' understanding, facilitating them in task performance, and increasing understanding on the part of the students in various aspects of language learning - including content, vocabulary, grammar, stories, poems, and cultural concepts. Drawing on the teachers' convictions, we have advanced that the L1 (here, Nepali) does play the role of cognitive bridging in English language learning, particularly when the students have a 'poor English base' (as perceived by the participants in this study), and when both the teacher and the students share a common L1. Hence, the general implication could be that the L1 can function as a support in teaching and learning a second/foreign language (L2). [ABSTRACT FROM AUTHOR]

Published
2023
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107. Effect of the nitrile group in extraction and bulk liquid membrane transport of amino acids.

Author

Billore, Shikha and Sharma, Uma

Subjects
NITRILES, LIQUID membranes, BIOLOGICAL transport, AMINO acids, ANTHRAQUINONES, BENZONITRILE
Abstract

Extraction and transport of amino acids through a liquid membrane system is an emerging field of supramolecular as well as biomedical science. In this piece of work, series of podands are used with triethylene and tetraethylene glycol chains having benzonitrile (PBT3, PBT4), anthraquinone (PAT3, PAT4) and naphthyl (PNT3, PNT4) end groups. Extraction and transport studies performed with amino acids: glycine, valine and threonine using these receptors. From the results, it is observed that the extraction efficiency of PBT3 and PBT4 is double than PNT3 and PNT4 podands, and also transport ability is double in PAT3, and PAT4. Thus we conclude that the nitrile group-containing podands (PBT3 and PBT4) are better extractants as well as a carrier than PAT3, PAT4, PNT3, PNT4, in which nitrile groups is a key component for molecular recognition of glycine, valine, and threonine, which are further used in the separation of amino acids. [ABSTRACT FROM AUTHOR]

Published
2023
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108. Aloe vera Nanoparticles Loaded with Antihypertensive Beta-Blockers of Different Half-Life: Entrapment Efficiency and Release Behavior.

Author

SHARMA, RACHANA, SHARMA, UMA, and PASWAN, S. K.

Subjects
*ALOE vera, *NANOCARRIERS, *FOURIER transform spectroscopy, *ADRENERGIC beta blockers, *X-ray powder diffraction, *NANOPARTICLE size, *NANOPARTICLES
Abstract

Biopolymeric nanocarriers have become potential candidates for drug delivery applications due to outstanding virtues such as biodegradability, biocompatibility, low toxicity and low cost. The present study involves the synthesis of such biopolymeric nanocarriers prepared from Aloe vera gel, conjugated with antihypertensive model drugs (atenolol, bisoprolol and propranolol) using ionotropic gelation polyelectrolyte complexation and the determination of entrapment efficiencies, in vitro release of these drugs from nanoparticles followed by the study of release kinetics. The synthesized antihypertensive drug-loaded nanoparticles were undertaken for characterization using Ultraviolet-Visible spectroscopy, Scanning Electron Microscopy, Fourier Transform Infra-red Spectroscopy and Zeta potential measurements and Powder X-ray diffraction analysis. The analysis ended up with confirmation of the semi-crystalline nature of the synthesized drug-loaded nanoparticles with an average size of 86.97±2.17 nm, 78.32±1.97 nm and 89.36±2.15 nm for atenolol, bisoprolol and propranolol loaded Aloe vera (gel) nanoparticles respectively determined with X-ray diffraction analysis. Atenolol and Bisoprolol loaded Aloe vera gel nanoparticles using barium chloride and Propranolol loaded Aloe vera gel nanoparticles using barium chloride followed Korsmeyer-Peppa’s model for drug release with Anomalous (non Fickian) transport. [ABSTRACT FROM AUTHOR]

Published
2023
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113. Expression of Estrogen and Progesterone Receptors in Ovarian Neoplasms

Author

Faizah, Sweta, Sharma Uma, and Aziz Mir Rizwan

Subjects
Aging, medicine.drug_class, Biology, Health Professions (miscellaneous), Biochemistry, Genetics and Molecular Biology (miscellaneous), General Biochemistry, Genetics and Molecular Biology, Expression (architecture), Estrogen, General Health Professions, medicine, Cancer research, Dentistry (miscellaneous), Receptor, General Dentistry
Published
2021
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116. Realization and performance evaluation of FIR filters using reversible logic.

Author

Kumar, Umesh, Sharma, Uma, Gupta, Sunil, and Bhaladhare, Pawan R.

Subjects
*FINITE impulse response filters, *DIGITAL signal processing, *DIGITAL electronics, *IMPULSE response, *REVERSIBLE computing, *LOGIC design
Abstract

To format your Reversible logic computation is an emerging field of research and findings, having anonymous applications in Quantum based computing and Nanotechnology. Reversible logic design is used to implement various digital circuits and becomes more interesting area due to its implication in reduced power consumption capability. It has been observed that with the escalating increase in transistor density, the consumption of power will reach beyond the saturation point somehow in conventional technology. Reversible logic gates are used for designing bigger and much complex circuits like adders, multipliers, filters and many more than that, resulting reduced energy consumption and heat dissipation. A wide range of applications depends on digital signal processor, used FIR ("finite impulse response") filters as a working unit for their functioning. Reversible logic basically used for improving the performance and speedup of a battery powered device. This paper describes a wide range of Vedic reversible and irreversible FIR filters along with their performance analysis. [ABSTRACT FROM AUTHOR]

Published
2022
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130. The effect of ketamine on the onset time of neuromuscular blockade with rocuronium bromide.

Author

Rajpurohit, Laxman Singh, Thanvi, Abhilasha, Sharma, Kashmira, Chouhan, Anita, and Sharma, Uma Dutt

Subjects
ROCURONIUM bromide, NEUROMUSCULAR blockade, KETAMINE, OXIMETRY, PREMEDICATION, DIASTOLIC blood pressure, SYSTOLIC blood pressure
Abstract

Background: The role of ketamine given before induction agents in shortening the onset time of neuromuscular blockade with rocuronium bromide can be of use in anesthesia practice. Aim: The aim of the study was to know the effect of ketamine on the time of onset of neuro-muscular block for intubation with rocuronium bromide. Method: Fifty patients of age between 18-60 years and ASA grading I/II undergoing elective surgery under general anaesthesia who gave valid informed consent were selected, After premedication, patients in Group I received normal saline in 10ml syringe, and group-II received Ketamine 0.5 mg kg-1 in 10 ml syringe. After one minute injection rocuronium in the dose of 0.9 mg kg-1 was administered. The primary outcome noted was effect of ketamine on the time for onset of neuromuscular block and secondary outcomes were any changes in heart rate, systolic and diastolic blood pressure, mean arterial pressure or pulse oximetry values) due to ketamine with rocuronium bromide and effect on duration of neuromuscular block with ketamine and rocuronium bromide. Results The mean onset time of neuromuscular block was more in Group I (control) compared to Group II (ketamine) with p value 0.04. Both groups remained hemodynamically stable. Conclusion: This study revealed that ketamine reduces the onset time of rocuronium for intubation while maintaining stable haemodynamics. [ABSTRACT FROM AUTHOR]

Published
2023
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141. Effect of addition of dexamethasone to ropivacaine on post-operative analgesia in ultrasonography-guided transversus abdominis plane block for inguinal hernia repair: A prospective, double-blind, randomised controlled trial

Author

Sharma, Uma, Prateek, and Tak, Himani

Subjects
Clinical trials -- Analysis, Ultrasound imaging -- Usage, Inguinal hernia -- Research -- Drug therapy -- Diagnosis, Dexamethasone -- Dosage and administration, Health
Abstract

Byline: Uma. Sharma, Prateek, Himani. Tak Background and Aims: Ultrasonography (USG)-guided transversus abdominis plane (TAP) block is an abdominal field block with high efficacy. This study was undertaken with the [...]

Published
2018

146. Subclinical Iron Overload Hampers the Beneficial Effect of Human Pegivirus (Hpgv) On Disease Progression in Human Immunodeficiency Virus Type-1 Infected Blood Donors

Author

Chattopadhya Debasish, Rai Arvind, Sharma Uma, and Verghese Alice

Subjects
biology, business.industry, Pegivirus, Disease progression, Human immunodeficiency virus (HIV), medicine, biology.organism_classification, medicine.disease_cause, business, Virology, Subclinical infection
Published
2021
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147. 6-GINGEROL AND PHYSICOCHEMICAL PROPERTIES AS MONITORING PARAMETER FOR A NATURAL GROWTH PROMOTER NBIOTIC™ PREMIX DURING FEED PELLETIZATION STRESS

Author

Ravikanth Kotagiri , Anirudh Sharma , Uma Ranjan Lal , Pushap Lata and Deepak Thakur

Subjects
Nbiotic™ Premix 6-Gingerol Stability Pelletization Chromatographic techniques
Abstract

Background:Feed is an important input for poultry and swine production, representing 50–70% of the total production costs. Feed processing provides an opportunity to improve poultry and swine performance. Herbal gut function modulators are used in poultry and swine feeds to improve nutrient utilization and efficiencies of biomass production. Nbiotic™ Premix is a phytoformulation of AYURVET LIMITED, INDIA used in poultry and swine as a natural growth promoter (NGP) to effectively replace antibiotic growth promoters (AGP). Pelletization used to prevent wastage of feed, reduce bulk and improve digestibility and palatability. In present work we have assessed the stability of the Nbiotic™ Premix herbal formulation under different pelletization thermal stress conditions. Methods:Formulation was kept under controlled temperature (90oC) and moisture (2.0 % w/w) for different time intervals (0, 5, 10 minutes). Chromatographic techniques (HPLC and HPTLC) and physicochemical properties were used to monitor the formulations stability. Nbiotic™ Premix was stable at all the experimental investigation of pelletization thermal stress. Results:No significant change in the 6-gingerol content (250.0 ppm (± 2.0 % at RT 10.05 minutes) and difference between peak areas and the retention factor (RF) values for the control samples and various thermal stress conditioned samples were observed.A comprehensive study of HPLC and HPTLC measurements have been applied for assessing the stability of Nbiotic™ Premix. Developed methods (HPLC & HPTLC) were found to be rapid, sensitive and reliable in determining its stability. As evident, Nbiotic™ Premix can undergo pelletization process without any degradation. In conclusion, present study will help towards developing appropriate stability monitoring parameters for herbal products which is a major challenge in the nutraceutical industry in current scenario. &nbsp

Published
2021
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