101. Expression of bcl-2, p53 oncoprotein, and proliferating cell nuclear antigen in renal cell carcinoma.
- Author
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Sejima T and Miyagawa I
- Subjects
- Apoptosis, Biomarkers, Tumor metabolism, Carcinoma, Renal Cell mortality, Female, Humans, Immunohistochemistry, Kidney metabolism, Kidney Neoplasms mortality, Male, Middle Aged, Prognosis, Survival Analysis, Carcinoma, Renal Cell metabolism, Kidney Neoplasms metabolism, Proliferating Cell Nuclear Antigen biosynthesis, Proto-Oncogene Proteins c-bcl-2 biosynthesis, Tumor Suppressor Protein p53 biosynthesis
- Abstract
Objectives: This study was designed to examine the immunohistochemical expression of bcl-2, p53, and proliferating cell nuclear antigen (PCNA) and the relation of this expression to clinicopathological characteristics and prognosis in renal cell carcinoma (RCC)., Methods: The expression of bcl-2, p53 protein, and PCNA was studied by immunohistochemical methods in paraffin-embedded nephrectomy specimens from 53 patients whose clinicopathological data had already become clear., Results: The expression of the bcl-2 protein was recognized in 34 cases (64%); the expression of the p53 protein, however, was seen in only 1 case. Bcl-2 positivity was not associated with any pathological parameters or prognosis. If the percentage of PCNA-positive cancer cells as compared to the total amount of cancer cells was defined as a labeling index (LI), a high PCNA LI number correlated significantly with a high T category, high grade, venous invasion, and shortened survival. Among the conventional pathological parameters, the T category, nuclear grade, and venous invasion had the most significant effect on prognosis. A multivariate analysis in the parameters of PCNA, T category, nuclear grade, and venous invasion demonstrated that only nuclear grade had a significant effect on prognosis., Conclusions: The inhibitory effect of the bcl-2 gene on apoptosis related to tumor development is not clear, and the expression of the p53 protein is uncommon in RCC. PCNA seems to be a good objective and quantitative marker of the biological malignant potential in RCC, although the assessment of malignant potential in combination with conventional pathological parameters is indispensable.
- Published
- 1999
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