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Since the arrival of DNA nanotechnology nearly 40 years ago, the field has progressed from its beginnings of envisioning rather simple DNA structures having a branched, multi-strand architecture into creating beautifully complex structures comprising hundreds or even thousands of unique strands, with the possibility to exactly control the positions down to the molecular level. While the earliest construction methodologies, such as simple Holliday junctions or tiles, could reasonably be designed on pen and paper in a short amount of time, the advent of complex techniques, such as DNA origami or DNA bricks, require software to reduce the time required and propensity for human error within the design process. Where available, readily accessible design software catalyzes our ability to bring techniques to researchers in diverse fields and it has helped to speed the penetration of methods, such as DNA origami, into a wide range of applications from biomedicine to photonics. Here, we review the historical and current state of CAD software to enable a variety of methods that are fundamental to using structural DNA technology. Beginning with the first tools for predicting sequence-based secondary structure of nucleotides, we trace the development and significance of different software packages to the current state-of-the-art, with a particular focus on programs that are open source.

Plant species have been introduced increasingly into non‐native ranges, where many have become exotic weeds with adverse impacts on native ecosystems, as well as on farming and other livelihoods. In biological control, the classical or inoculative approach is the one most commonly used for the management of invasive alien weeds and is based on the use of co‐evolved natural enemies from the native range to control the invasive weed. Typically, the inundative or mycoherbicide approach targets problematic weeds using local plant pathogens that, in the case of introduced species, have 'jumped' onto the exotic host. The leaf‐spot fungus, Mycosphaerella polygoni‐cuspidati, co‐evolved with its host, Reynoutria (Fallopia) japonica (Japanese knotweed), in Japan and has a unique history of being investigated both as a classical biological control agent and a mycoherbicide against this highly invasive weed in the United Kingdom and North America. Here, we highlight our research on M. polygoni‐cuspidati as part of a biological control programme for Japanese knotweed and review the potential of mycoherbicides using exotic pathogens for the management of invasive alien weeds. © 2023 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

The Jostedalsbreen is the largest ice cap in Norway and mainland Europe. Rapid retreat of many of its outlet glaciers since the 2000s has led to the formation of several glacial lakes. Processes causing the formation and expansion of glacial lakes and their interaction with a glacier and terminal moraine have not been widely addressed yet. In this study, we investigate the degradation of the front of the southeast‐facing outlet glacier Austerdalsbreen. Based on a variety of remotely sensed data (UAV‐based and airborne orthophotos and DEMs, satellite images), we analyze the coincident glacial and proglacial changes of Austerdalsbreen and quantify the evolution of this transition zone during the last decades. In particular, we focus on the short‐term evolution of the glacial lake since 2010, we examine the context of a glacier advance in the 1990s, and we report long‐term changes by utilizing 1960s imagery. We discuss the evolution and conditions of Austerdalsbreen compared to other outlet glaciers of Jostedalsbreen. Overall, the glacier terminus has experienced a recession in the last decades. The 1990s terminus advance was more restricted than at other nearby outlet glaciers due to glacier surface debris cover, which is a critical factor for the glacier and lake evolution. However, in the most recent period, since 2012, a distinct expansion of a glacial lake is quantifiable. Since the rates of glacier surface lowering also considerably increased since approximately 2017 and the glacier retreated since the beginning of the 2000s with a clear maximum length decrease in 2015, we interpret the recently formed glacial lake as a contributory factor of glacial changes. [ABSTRACT FROM AUTHOR]

Purpose: Enucleation is a common treatment modality performed for pediatric retinoblastoma patients, and the resultant defects are reconstructed using an ocular prosthesis. The prostheses are modified or replaced periodically, as the child develops due to orbital growth and patient‐error. The purpose of this report is to evaluate the replacement frequency of prostheses in the pediatric oncologic population. Methods: A retrospective review was completed by the two senior research investigators, of patients that had ocular prostheses fabricated following enucleation of their retinoblastoma from 2005 to 2019 (n = 90). Data collected from the medical records of the patient included the pathology, date of surgery, date of prosthesis delivery, and the replacement schedule of the ocular prosthesis. Results: During the 15‐year study period, 78 enucleated observations (ocular prosthesis fabricated) were included for analysis. The median age of the patients at the time of delivery of their first ocular prosthesis was calculated to be 2.6 years (range 0.3–18 years). The median time to the first modification of the prosthesis was calculated to be 6 months. The time to modification of the ocular prosthesis was further stratified by age. Conclusion: Pediatric patients require modification of their ocular prostheses throughout their growth and development period. Ocular prostheses are reliable prostheses with predictable outcomes. This data is helpful to set an expectation among the patient, parent, and provider. [ABSTRACT FROM AUTHOR]