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Your search keyword '"Scherphof, G. L."' showing total 105 results
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105 results on '"Scherphof, G. L."'

101. Exchange of phospholipids between microsomes and inner and outer mitochondrial membranes of rat liver.

Author

Blok MC, Wirtz KW, and Scherphof GL

Subjects
Adenine Nucleotides, Animals, Endoplasmic Reticulum metabolism, Ligases metabolism, Male, Microscopy, Electron, Phosphates metabolism, Phosphatidylcholines biosynthesis, Phosphatidylethanolamines biosynthesis, Phosphorus Isotopes, Phosphotransferases metabolism, Rats, Surface-Active Agents pharmacology, Membranes metabolism, Microsomes, Liver metabolism, Mitochondria, Liver metabolism, Phosphatidylcholines metabolism, Phosphatidylethanolamines metabolism
Published
1971
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102. On the pathways of fatty acid incorporation into the lipids of subcellular particles of rat liver and into erythrocytes.

Author

Scherphof GL and van Deenen LL

Subjects
Animals, Carbon Isotopes metabolism, Chromatography, Paper, In Vitro Techniques, Liver cytology, Rabbits, Rats, Subcellular Fractions metabolism, Tritium metabolism, Erythrocytes metabolism, Glycerophosphates metabolism, Microsomes metabolism, Mitochondria metabolism, Palmitic Acids metabolism, Phosphatidylcholines biosynthesis, Phosphatidylethanolamines biosynthesis
Published
1966
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103. Differentiation of phospholipases A in mitochondria and lysosomes of rat liver.

Author

Waite M, Scherphof GL, Boshouwers FM, and van Deenen LL

Subjects
Adenine Nucleotides pharmacology, Adenosine Triphosphate pharmacology, Animals, Carbon Isotopes, Choline, Depression, Chemical, Fatty Acids, Nonesterified pharmacology, Hydrogen-Ion Concentration, Kinetics, Liver cytology, Magnesium pharmacology, Male, Phosphatidylcholines, Phosphatidylethanolamines, Phospholipases antagonists & inhibitors, Phosphorus Isotopes, Rats, Stimulation, Chemical, Liver enzymology, Lysosomes enzymology, Mitochondria, Liver enzymology, Phospholipases metabolism
Abstract

Highly purified mitochondria from rat liver contain a phospholipase A that catalyzes removal of 2-fatty acids, with a pH optimum above pH 8.0. Lysosomal preparations appeared to have two phospholipases A associated with them, one with a pH optimum at about pH 4.0, the second between pH 6.0 and 7.0. Mitochondrial phospholipase A hydrolyzed exogenous phospholipid as fast as or faster than endogenous phospholipid. The difference in specific radioactivity of (14)C-ethanolamine-labeled endogenous mitochondrial phospholipid before and after incubation indicates that a fraction of mitochondrial phosphatidyl ethanolamine is hydrolyzed more rapidly than the mitochondrial phospholipids as a whole. Acyl bond hydrolysis of exogenous and endogenous phospholipid by mitochondria was stimulated by free fatty acid, Ca(++), or in certain cases, monoacyl phospholipids or by treatments that disrupt the mitochondrial membrane. Of various fatty acids tested, lauric, myristic, oleic, and linoleic were most effective. ADP and ATP inhibited mitochondrial phospholipase, probably because they compete for Ca(++). Mg(++) also behaved as a competitive inhibitor; the effect was overcome by relatively little Ca(++).

Published
1969

104. The effect of local anesthetics on the hydrolysis of free and membrane-bound phospholipids catalyzed by various phospholipases.

Author

Scherphof GL, Scarpa A, and van Toorenenbergen A

Subjects
Amino Alcohols, Animals, Bacillus cereus enzymology, Calcium pharmacology, Carbon Isotopes, Chromatography, Thin Layer, Egg Yolk, Enzyme Activation, Female, Kinetics, Microsomes, Liver enzymology, Mitochondria, Liver metabolism, Oxygen Consumption drug effects, Pancreas enzymology, Phosphatidylcholines, Phospholipases pharmacology, Procaine, Rats, Spectrophotometry, Ultraviolet Rays, Anesthetics, Local pharmacology, Microsomes, Liver drug effects, Mitochondria, Liver drug effects, Phospholipases antagonists & inhibitors
Published
1972
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105. In vitro stimulation of lecithin synthesis in rat liver mitochondria and microsomes after treatment with phospholipase C.

Author

van Schijndel BC, Reitsema A, and Scherphof GL

Subjects
Animals, Bacillus cereus enzymology, Carbon Radioisotopes, Choline, Cytosine Nucleotides, Glycerides biosynthesis, Kinetics, Male, Microsomes, Liver drug effects, Mitochondria, Liver drug effects, Rats, Time Factors, Microsomes, Liver metabolism, Mitochondria, Liver metabolism, Phosphatidylcholines biosynthesis, Phospholipases pharmacology
Published
1973
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