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101. Diffusion ordered spectroscopy as a complement to size exclusion chromatography in oligosaccharide analysis

Author

Groves, P., Rasmussen, M.O., Molero, M.D., Samain, E., CaÑada, F.J., Driguez, H., Jimenez-Barbero, J., Centre de Recherches sur les Macromolécules Végétales (CERMAV), and Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)

Subjects
ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2004

102. Assessment of the two Helicobacter pylori α-1,3-fucosyltransferase ortholog genes for the large-scale synthesis of LewisX human milk oligosaccharides by metabolically engineered Escherichia coli

Author

Dumon, C., Samain, E., Priem, B., Centre de Recherches sur les Macromolécules Végétales (CERMAV), and Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)

Subjects
ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2004

103. A Mission to Explore the Pioneer Anomaly

Author

The Pioneer Explorer Collaboration, Dittus, H., Turyshev, S. G., L��mmerzahl, C., Theil, S., Foerstner, R., Johann, U., Ertmer, W., Rasel, E., Dachwald, B., Seboldt, W., Hehl, F. W., Kiefer, C., Blome, H. -J., Kunz, J., Giulini, D., Bingham, R., Kent, B., Sumner, T. J., Bertolami, O., P��ramos, J., Rosales, J. L., Christophe, B., Foulon, B., Touboul, P., Bouyer, P., Reynaud, S., Brillet, A., Bondu, F., Samain, E., de Matos, C. J., Erd, C., Grenouilleau, J. C., Izzo, D., Rathke, A., Anderson, J. D., Asmar, S. W., Lau, E. L., Nieto, M. M., Mashhoon, B., Jet Propulsion Laboratory (JPL), NASA-California Institute of Technology (CALTECH), ONERA - The French Aerospace Lab [Châtillon], ONERA-Université Paris Saclay (COmUE), laboratoire Charles Fabry de l'Institut d'Optique / Optique atomique, Laboratoire Charles Fabry de l'Institut d'Optique (LCFIO), Centre National de la Recherche Scientifique (CNRS)-Institut d'Optique Graduate School (IOGS)-Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS)-Institut d'Optique Graduate School (IOGS)-Université Paris-Sud - Paris 11 (UP11), Laboratoire Kastler Brossel (LKB (Jussieu)), Université Pierre et Marie Curie - Paris 6 (UPMC)-Fédération de recherche du Département de physique de l'Ecole Normale Supérieure - ENS Paris (FRDPENS), École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Astrophysique Relativiste Théories Expériences Métrologie Instrumentation Signaux (ARTEMIS), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire de la Côte d'Azur, Université Côte d'Azur (UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS), Los Alamos National Laboratory (LANL), Jet Propulsion Laboratory ( JPL ), NASA-California Institute of Technology ( CALTECH ), ONERA - The French Aerospace Lab ( Chatillon ), ONERA, Laboratoire Charles Fabry de l'Institut d'Optique ( LCFIO ), Université Paris-Sud - Paris 11 ( UP11 ) -Institut d'Optique Graduate School ( IOGS ) -Centre National de la Recherche Scientifique ( CNRS ) -Université Paris-Sud - Paris 11 ( UP11 ) -Institut d'Optique Graduate School ( IOGS ) -Centre National de la Recherche Scientifique ( CNRS ), Laboratoire Kastler Brossel ( LKB (Jussieu) ), Fédération de recherche du Département de physique de l'Ecole Normale Supérieure - ENS Paris ( FRDPENS ), Centre National de la Recherche Scientifique ( CNRS ) -École normale supérieure - Paris ( ENS Paris ) -Centre National de la Recherche Scientifique ( CNRS ) -École normale supérieure - Paris ( ENS Paris ) -Université Pierre et Marie Curie - Paris 6 ( UPMC ) -Centre National de la Recherche Scientifique ( CNRS ), Astrophysique Relativiste Théories Expériences Métrologie Instrumentation Signaux ( ARTEMIS ), Université Nice Sophia Antipolis ( UNS ), Université Côte d'Azur ( UCA ) -Université Côte d'Azur ( UCA ) -Institut national des sciences de l'Univers ( INSU - CNRS ) -Observatoire de la Côte d'Azur, Université Côte d'Azur ( UCA ) -Centre National de la Recherche Scientifique ( CNRS ), Los Alamos National Laboratory ( LANL ), Université Paris-Sud - Paris 11 (UP11)-Institut d'Optique Graduate School (IOGS)-Centre National de la Recherche Scientifique (CNRS)-Université Paris-Sud - Paris 11 (UP11)-Institut d'Optique Graduate School (IOGS)-Centre National de la Recherche Scientifique (CNRS), Fédération de recherche du Département de physique de l'Ecole Normale Supérieure - ENS Paris (FRDPENS), École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), Université Nice Sophia Antipolis (1965 - 2019) (UNS), and COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Université Côte d'Azur (UCA)-Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[PHYS.GRQC]Physics [physics]/General Relativity and Quantum Cosmology [gr-qc], FOS: Physical sciences, General Relativity and Quantum Cosmology (gr-qc), General Relativity and Quantum Cosmology, [ PHYS.GRQC ] Physics [physics]/General Relativity and Quantum Cosmology [gr-qc]
Abstract

The Pioneer 10 and 11 spacecraft yielded the most precise navigation in deep space to date. These spacecraft had exceptional acceleration sensitivity. However, analysis of their radio-metric tracking data has consistently indicated that at heliocentric distances of $\sim 20-70$ astronomical units, the orbit determinations indicated the presence of a small, anomalous, Doppler frequency drift. The drift is a blue-shift, uniformly changing with a rate of $\sim(5.99 \pm 0.01)\times 10^{-9}$ Hz/s, which can be interpreted as a constant sunward acceleration of each particular spacecraft of $a_P = (8.74 \pm 1.33)\times 10^{-10} {\rm m/s^2}$. This signal has become known as the Pioneer anomaly. The inability to explain the anomalous behavior of the Pioneers with conventional physics has contributed to growing discussion about its origin. There is now an increasing number of proposals that attempt to explain the anomaly outside conventional physics. This progress emphasizes the need for a new experiment to explore the detected signal. Furthermore, the recent extensive efforts led to the conclusion that only a dedicated experiment could ultimately determine the nature of the found signal. We discuss the Pioneer anomaly and present the next steps towards an understanding of its origin. We specifically focus on the development of a mission to explore the Pioneer Anomaly in a dedicated experiment conducted in deep space., 8 pages, 9 figures; invited talk given at the 2005 ESLAB Symposium "Trends in Space Science and Cosmic Vision 2020", 19-21 April 2005, ESTEC, Noordwijk, The Netherlands

Published
2004

104. Fundamental Physics with the Laser Astrometric Test Of Relativity

Author

LATOR Collaboration, Turyshev, S. G., Dittus, H., Shao, M., Nordtvedt, Jr., K. L., Laemmerzahl, C., Theil, S., Ertmer, W., Rasel, E., Foerstner, R., Johann, U., Klioner, S., Soffel, M., Dachwald, B., Seboldt, W., Perlick, V., Sandford, M. C. W., Bingham, R., Kent, B., Sumner, T. J., Bertolami, O., Paramos, J., Christophe, B., Foulon, B., Touboul, P., Bouyer, P., Damour, T., Reynaud, S., Salomon, C., Brillet, A., Bondu, F., Mangin, J. -F., Samain, E., Erd, C., Grenouilleau, J. C., Izzo, D., Rathke, A., Asmar, S. W., Colavita, M., Gursel, Y., Jet Propulsion Laboratory (JPL), NASA-California Institute of Technology (CALTECH), ONERA - The French Aerospace Lab [Châtillon], ONERA-Université Paris Saclay (COmUE), laboratoire Charles Fabry de l'Institut d'Optique / Optique atomique, Laboratoire Charles Fabry de l'Institut d'Optique (LCFIO), Centre National de la Recherche Scientifique (CNRS)-Institut d'Optique Graduate School (IOGS)-Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS)-Institut d'Optique Graduate School (IOGS)-Université Paris-Sud - Paris 11 (UP11), Institut des Hautes Etudes Scientifiques (IHES), IHES, Laboratoire Kastler Brossel (LKB (Jussieu)), Université Pierre et Marie Curie - Paris 6 (UPMC)-Fédération de recherche du Département de physique de l'Ecole Normale Supérieure - ENS Paris (FRDPENS), École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Kastler Brossel (LKB (Lhomond)), Astrophysique Relativiste Théories Expériences Métrologie Instrumentation Signaux (ARTEMIS), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire de la Côte d'Azur, Université Côte d'Azur (UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS), Jet Propulsion Laboratory ( JPL ), NASA-California Institute of Technology ( CALTECH ), ONERA - The French Aerospace Lab ( Chatillon ), ONERA, Laboratoire Charles Fabry de l'Institut d'Optique ( LCFIO ), Université Paris-Sud - Paris 11 ( UP11 ) -Institut d'Optique Graduate School ( IOGS ) -Centre National de la Recherche Scientifique ( CNRS ) -Université Paris-Sud - Paris 11 ( UP11 ) -Institut d'Optique Graduate School ( IOGS ) -Centre National de la Recherche Scientifique ( CNRS ), Institut des Hautes Etudes Scientifiques ( IHES ), Laboratoire Kastler Brossel ( LKB (Jussieu) ), Fédération de recherche du Département de physique de l'Ecole Normale Supérieure - ENS Paris ( FRDPENS ), Centre National de la Recherche Scientifique ( CNRS ) -École normale supérieure - Paris ( ENS Paris ) -Centre National de la Recherche Scientifique ( CNRS ) -École normale supérieure - Paris ( ENS Paris ) -Université Pierre et Marie Curie - Paris 6 ( UPMC ) -Centre National de la Recherche Scientifique ( CNRS ), Laboratoire Kastler Brossel ( LKB (Lhomond) ), Astrophysique Relativiste Théories Expériences Métrologie Instrumentation Signaux ( ARTEMIS ), Université Nice Sophia Antipolis ( UNS ), Université Côte d'Azur ( UCA ) -Université Côte d'Azur ( UCA ) -Institut national des sciences de l'Univers ( INSU - CNRS ) -Observatoire de la Côte d'Azur, Université Côte d'Azur ( UCA ) -Centre National de la Recherche Scientifique ( CNRS ), Université Paris-Sud - Paris 11 (UP11)-Institut d'Optique Graduate School (IOGS)-Centre National de la Recherche Scientifique (CNRS)-Université Paris-Sud - Paris 11 (UP11)-Institut d'Optique Graduate School (IOGS)-Centre National de la Recherche Scientifique (CNRS), Institut des Hautes Études Scientifiques (IHES), Fédération de recherche du Département de physique de l'Ecole Normale Supérieure - ENS Paris (FRDPENS), École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), Université Nice Sophia Antipolis (1965 - 2019) (UNS), and COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Université Côte d'Azur (UCA)-Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Physics::Space Physics, [PHYS.GRQC]Physics [physics]/General Relativity and Quantum Cosmology [gr-qc], FOS: Physical sciences, General Relativity and Quantum Cosmology (gr-qc), General Relativity and Quantum Cosmology, [ PHYS.GRQC ] Physics [physics]/General Relativity and Quantum Cosmology [gr-qc]
Abstract

The Laser Astrometric Test Of Relativity (LATOR) is a joint European-U.S. Michelson-Morley-type experiment designed to test the pure tensor metric nature of gravitation - a fundamental postulate of Einstein's theory of general relativity. By using a combination of independent time-series of highly accurate gravitational deflection of light in the immediate proximity to the Sun, along with measurements of the Shapiro time delay on interplanetary scales (to a precision respectively better than 0.1 picoradians and 1 cm), LATOR will significantly improve our knowledge of relativistic gravity. The primary mission objective is to i) measure the key post-Newtonian Eddington parameter \gamma with accuracy of a part in 10^9. (1-\gamma) is a direct measure for presence of a new interaction in gravitational theory, and, in its search, LATOR goes a factor 30,000 beyond the present best result, Cassini's 2003 test. The mission will also provide: ii) first measurement of gravity's non-linear effects on light to ~0.01% accuracy; including both the Eddington \beta parameter and also the spatial metric's 2nd order potential contribution (never measured before); iii) direct measurement of the solar quadrupole moment J2 (currently unavailable) to accuracy of a part in 200 of its expected size; iv) direct measurement of the "frame-dragging" effect on light by the Sun's gravitomagnetic field, to 1% accuracy. LATOR's primary measurement pushes to unprecedented accuracy the search for cosmologically relevant scalar-tensor theories of gravity by looking for a remnant scalar field in today's solar system. We discuss the mission design of this proposed experiment., Comment: 8 pages, 9 figures; invited talk given at the 2005 ESLAB Symposium "Trends in Space Science and Cosmic Vision 2020," 19-21 April 2005, ESTEC, Noodrwijk, The Netherlands

Published
2004

111. Impact des prescriptions d’antifongique sur l’épidémiologie à Candida dans un service de réanimation chirurgicale suivant une stratégie préemptive contre les Candidoses : suivi prospectif systématique sur 8ans

Author

Ferreira, D., primary, Grenouillet, F., additional, Hénon, T., additional, Dussaucy, A., additional, Blasco, G., additional, Millon, L., additional, Samain, E., additional, Mercier, M., additional, and Pili Floury, S., additional

Published
2014
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116. A sub-ns comparison between GPS common view and T2L2

Author

Samain, E., primary, Laas-Bourez, M., additional, Courde, C., additional, Exertier, P., additional, Martin, N., additional, Torre, J.-M., additional, Rovera, G. D., additional, Abgrall, M., additional, Uhrich, P., additional, Guillemot, Ph., additional, Sherwood, R. A., additional, Appleby, G., additional, and Fridelance, P., additional

Published
2014
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118. Large-scale in vivo synthesis of the carbohydrate moieties of gangliosides GM1 and GM2 by metabolically engineered Escherichia coli

Author

Antoine, T., Priem, B., Heyraud, A., Greffe, L., Gilbert, M., Wakarchuk, W.W., Lam, J.S., Samain, E., Centre de Recherches sur les Macromolécules Végétales (CERMAV), and Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)

Subjects
ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2003

120. Method for producing oligopolysaccharides

Author

Samain, E., Priem, B., Centre de Recherches sur les Macromolécules Végétales (CERMAV), and Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)

Published
2001

121. In vivo fucosylation of lacto-N-neotetraose and lacto-N-neohexaose by heterologous expression of Helicobacter pylori α-1,3 fucosyltransferase in engineered Escherichia coli

Author

Dumon, C., Priem, B., Martin, S.L., Heyraud, A., Bosso, C., Samain, E., Centre de Recherches sur les Macromolécules Végétales (CERMAV), and Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)

Subjects
ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2001

124. Procédé de production d'oligosaccharides

Author

Samain, E., Centre de Recherches sur les Macromolécules Végétales (CERMAV), and Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)

Published
1999

125. The living factory: in vivo production of N-acetyllactosamine containing carbohydrates in E. coli

Author

Bettler, E., Samain, E., Chazalet, V., Bosso, C., Heyraud, A., Joziasse, Dh, Wakarchuk, Ww, Imberty, A., Geremia, Ar, Institut de biologie et chimie des protéines [Lyon] (IBCP), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), and Deleage, Gilbert

Subjects
Chromatography, Magnetic Resonance Spectroscopy, Carbohydrate Sequence, Molecular Sequence Data, N-Acetyllactosamine Synthase, [SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology, Escherichia coli, Oligosaccharides, Amino Sugars, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Cloning, Molecular
Abstract

International audience; Scientific and commercial interest in oligosaccharides is increasing, but their availability is limited as production relies on chemical or chemo-enzymatic synthesis. In search for a more economical, alternative procedure, we have investigated the possibility of producing specific oligosaccharides in E. coli that express the appropriate glycosyltransferases. The Azorhizobium chitin pentaose synthase NodC (a beta(1,4)GlcNAc-oligosaccharide synthase), and the Neisseria beta(1,4)galactosyltransferase LgtB, were co-expressed in E. coli. The major oligosaccharide isolated from the recombinant strain, was subjected to LC-MS, FAB-MS and NMR analysis, and identified as betaGal(1,4)[betaGlcNAc(1,4)]4GlcNAc. High cell density culture yielded more than 1.0 gr of the hexasaccharide per liter of culture. The compound was found to be an acceptor in vitro for betaGal(1,4)GlcNAc alpha(1,3)galactosyltransferase, which suggests that the expression of additional glycosyltransferases in E. coli will allow the production of more complex oligosaccharides.Scientific and commercial interest in oligosaccharides is increasing, but their availability is limited as production relies on chemical or chemo-enzymatic synthesis. In search for a more economical, alternative procedure, we have investigated the possibility of producing specific oligosaccharides in E. coli that express the appropriate glycosyltransferases. The Azorhizobium chitin pentaose synthase NodC (a beta(1,4)GlcNAc-oligosaccharide synthase), and the Neisseria beta(1,4)galactosyltransferase LgtB, were co-expressed in E. coli. The major oligosaccharide isolated from the recombinant strain, was subjected to LC-MS, FAB-MS and NMR analysis, and identified as betaGal(1,4)[betaGlcNAc(1,4)]4GlcNAc. High cell density culture yielded more than 1.0 gr of the hexasaccharide per liter of culture. The compound was found to be an acceptor in vitro for betaGal(1,4)GlcNAc alpha(1,3)galactosyltransferase, which suggests that the expression of additional glycosyltransferases in E. coli will allow the production of more complex oligosaccharides.

Published
1999

126. Chemical modification of poly(hydrioxyalkanoates). Copolymers bearing pendant sugars

Author

Constantin, M., Simionescu, C.I., Carpov, A., Samain, E., Driguez, H., Centre de Recherches sur les Macromolécules Végétales (CERMAV), and Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)

Subjects
ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
1999

133. T2L2: Five years in space

Author

Exertier, P., primary, Samain, E., additional, Courde, C., additional, Martin, N., additional, Torre, J.-M., additional, Oneto, J.-L., additional, Geoazur, M. Laas-Bourez, additional, Guillemot, Ph., additional, and Leon, S., additional

Published
2013
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136. Simultaneous production of two different gel-forming exopolysaccharides by an Alteromonas strain originating from deep sea hydrothermal vents

Author

Samain, E., Milas, M., Bozzi, L., Dubreucq, G., Rinaudo, M., Centre de Recherches sur les Macromolécules Végétales (CERMAV), and Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)

Subjects
ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
1997

141. Time Transfer by Laser Link — T2L2: Current status and future experiments

Author

Samain, E., primary, Albanese, D., additional, Courde, C., additional, Djeroud, K., additional, Bourez, M. Laaz, additional, Leon, S., additional, Mariey, H., additional, Martinot-Lagarde, G., additional, Oneto, J. L., additional, Paris, J., additional, Pierron, M., additional, Exertier, P., additional, Viot, H., additional, Guillemot, Ph., additional, Laurent, Ph., additional, Pierron, F., additional, Rovera, D., additional, Torre, J.-M., additional, Abgrall, M., additional, and Achkar, J., additional

Published
2011
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