101. Expression of the growth hormone-binding protein messenger RNA in the liver and extrahepatic tissues in the rat: co-expression with the growth hormone receptor.
- Author
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Carlsson B, Billig H, Rymo L, and Isaksson OG
- Subjects
- Age Factors, Animals, Carrier Proteins genetics, DNA genetics, Female, Gene Expression Regulation, Male, Mice, Organ Specificity, Rats, Receptors, Somatotropin genetics, Carrier Proteins biosynthesis, Liver metabolism, RNA, Messenger biosynthesis, Receptors, Somatotropin biosynthesis
- Abstract
A cDNA encoding a growth hormone-binding protein (GH-BP) was recently cloned from mouse and rat liver. The GH-BP in these species is identical to the extracellular part of the GH receptor (GH-R) with the transmembrane and intracellular domain substituted for a hydrophilic tail. In the present study the expression of the GH-BP and GH-R was studied in rat liver and extrahepatic tissues. Specific transcripts with estimated sizes of 1.2 kb (GH-BP) and 4.0 kb (GH-R) were found in the liver from both sexes. The expression of GH-BP increased with age up to puberty suggesting that it is developmentally regulated in a similar manner as GH-R. GH-BP mRNA was found in all extrahepatic tissues examined that contained GH-R mRNA. The ratio between the 1.2 kb and 4.0 kb transcripts varied between tissues indicating that GH-R and GH-BP transcripts may be separately regulated. The co-expression of GH-BP and GH-R suggests a functional role for the GH-BP in the local regulation of GH action.
- Published
- 1990
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