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392 results on '"Rubtsov, P. M."'

123. Interaction of the combustion front of methane-air mixture at low pressures with obstacles of cylindrical shape

Author

Rubtsov, Nickolai M., Chernysh, Victor I., Tsvetkov, Georgii I., and Troshin, Kirill Ya.

Abstract

•The interaction of flames with obstacles is valuable both for the development of reliable numerical models and for safety issues.•It was shown that the methane-oxygen flame does not form von Karman vortex street behind the cylindrical obstacle.•Von Karman instability occurs in the flow of products. In the perforated cylinder, ignition centers on its inner surface occur.•The model of compressible Navier–Stokes equations in a low Mach number approximation allows obtaining both instability modes.•The occurrence of local primary ignition centers on inner surface of the obstacle was qualitatively illustrated.

Published
2021
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125. Yeast Ribosomal Genes

Author

Bayev, A. A., Skryabin, K. G., Zaharyev, V. M., Krayev, A. S., Rubtsov, P. M., Salvatore, Francesco, editor, Marino, Gennaro, editor, and Volpe, Pietro, editor

Published
1979
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126. The role of HCV e2 protein glycosylation in functioning of virus envelope proteins in insect and mammalian cells

Author

Orlova, O. V., Drutsa, V. L., Spirin, P. V., Prasolov, V. S., Rubtsov, P. M., Sergey Kochetkov, and Beljelarskaya, S. N.

Subjects
carbohydrates (lipids), viruses, MAMMALIAN HEK293T AND HUH7.0 CELLS
Abstract

The hepatitis C virus (HCV) envelope proteins E1 and E2, being virion components, are involved in the formation of infectious particles in infected cells. The detailed structure of the infectious particle of HCV remains poorly understood. Moreover, the virion assembly and release of virions by the cell are the least understood processes. It is believed that virion properties depend on glycosylation of the virus envelope proteins in a cell, while glycansat several glycosylation sites of these proteins play a pivotal role in protein functioning and the HCV life cycle. N-glycans of glycoproteins can influence viral particle formation, virus binding to cell surface, and HCV pathogenesis. We studied the effect of glycans on the folding ofthe E2 glycoprotein, formation of functional glycoprotein complexes and virus particles in insect and mammalian cells. In order to investigate these processes, point mutations of the N-glycosylation sites of HCV protein E2 (genotype 1b strain 274933RU) were generated and the mutant proteins were further analyzed in the baculovirus expression system. Elimination of the single glycosylation sites of the E2 glycoprotein, except for the N6 site, did not affect its synthesis efficiency in Sf9 insect cells, while the electrophoretic mobility of mutant proteins increased in proportion to the decrease in the number of glycosylation sites. The level of synthesis of HCV glycoprotein E2 in human HEK293T cells depended on the presence of glycans at the N1 and N8 glycosylation sites in contrast to Sf9 cells. At the same time, elimination of glycans at the N1, N2, and N10 sites led to the accumulation of unproductive E1E2 dimers as aggregates and productive assembly suppression of virus-like particles both in insect and mammalian cells. In addition, elimination of single glycosylation sites of HCV E2 had no impact on the RNA synthesis of structural proteins and formation of virus-like particles in insect and mammalian cells.

Published
2015

127. Synthesis and Secretion of Mammalian Proteins in Yeast

Author

Eldarov, M. A., Tziomenko, A. B., Karpychev, I. V., Morzunov, S. P., Tchernov, B. K., Rubtsov, P. M., Kulaev, I. S., Skryabin, K. G., Bayev, A. A., Larionov, V. L., Eldarov, M. A., Tziomenko, A. B., Karpychev, I. V., Morzunov, S. P., Tchernov, B. K., Rubtsov, P. M., Kulaev, I. S., Skryabin, K. G., Bayev, A. A., Schmitt, H. D., Wagner, P., Molenaar, C., Brökel, R., Haubruck, H., Gallwitz, D., Efimov, V. A., Buryakova, A. A., Reverdatto, S. V., Chakhamakhcheva, O. G., Ovchinnikov, Yu. A., Saedler, H., Paz-Ares, J., Wienand, U., Schell, J., Broude, N. E., Sverdlov, E. D., Paves, H., Timmusk, T., Metsis, M., Kelve, M., Toots, U., Neuman, T., Saarma, M., Sakmann, B., Korobko, V. G., Graf, T., Beug, H., Golay, J., Leutz, A., Ness, S., Gruss, P., Gren, E., Borisova, G., Kozlovskaya, T., Pushko, P., Tsibinogin, V., Ose, V., Pumpen, P., Tanyashin, V. I., Georgiev, G. P., Shick, V., Belyavsky, A., Postnikov, Yu., Studitsky, V., Sippel, A. E., Theisen, M., Borgmeyer, U., Rupp, R. A. W., Strech-Jurk, U., Stief, A., Müller, A., Hecht, A., Grussenmeyer, T., Zasedatelev, A. S., Gursky, G. V., Grokhovsky, S. L., Gottikh, B. P., Zhuze, A. L., Hörz, W., Mirzabekov, A., Karpov, V., Preobrazhenskaya, O., Bavykin, S., Ebralidse, K., Vlassov, V. V., Zarytova, V. F., Zachau, H. G., Klobeck, H. G., Lötscher, E., Pargent, W., Pohlenz, H. D., Straubinger, B., Zimmer, F. J., Hofschneider, P. H., Wollersheim, M., Zahm, P., Zabarovsky, E. R., Nurbekov, M. K., Turina, O. V., Kisselev, L. L., Riva, M., Schäffner, A. R., Sentenac, A., Hartmann, G. R., Mustaev, A. A., Zaychikov, E. F., Grachev, M. A., Falkenburg, D., Bautz, E. K. F., Grummt, I., Krämer, A., Frendewey, D., Lahr, G., Christofori, G., Frick, M., and Keller, W.

Published
1987
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133. Abstracts of papers deposited at VINITI

Author

Gyunter, V. É., Monasevich, L. A., Paskal', Yu. I., Khachin, V. N., Grinchak, A. I., Makogon, M. M., Serov, V. V., Minaev, B. F., Lupik, A. N., Grigor'ev, A. I., Tikhomirov, I. A., Shishkovskii, V. I., Sergeev, V. N., Krokhin, S. N., Lopatkin, Yu. M., Skorobogat'ko, A. F., Golovleva, V. K., Detinko, V. N., Kleshchuk, V. G., Perfil'ev, V. I., Chernyavskii, V. G., Levina, V. B., Gavrilina, N. I., Bespalov, V. I., Kol'chuzhkin, A. M., Tikhomirov, I. A., Shishkovskii, V. I., Krokhin, S. N., Larionov, V. V., Tikhomirov, I. A., Larionov, V. V., Vergun, A. P., Yakubov, V. P., Golovnev, I. F., Epin, V. N., Baum, B. A., Tyagunov, G. B., Kudzin, A. Yu., Sadovskaya, L. Y., Poplavskii, I. V., Popushoi, M. N., Khitche, L., Klenov, É. N., Andrianov, A. S., Belan, D. P., Bogacheva, L. S., Klimov, B. N., Lykov, Yu. I., Lykova, S. M., Aleksankin, V. G., Kolobashkin, V. M., Rubtsov, P. M., Ruzhanskii, P. A., Natanzon, G. A., Blinkova, V. G., Popova, T. N., Naumov, A. P., and Potetyunko, G. N.

Published
1977
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137. Organization and Function of the Eucaryotic Genome

Author

Larionov, V. L., primary, Eldarov, M. A., additional, Tziomenko, A. B., additional, Karpychev, I. V., additional, Morzunov, S. P., additional, Tchernov, B. K., additional, Rubtsov, P. M., additional, Kulaev, I. S., additional, Skryabin, K. G., additional, Bayev, A. A., additional, Schmitt, H. D., additional, Wagner, P., additional, Molenaar, C., additional, Brökel, R., additional, Haubruck, H., additional, Gallwitz, D., additional, Efimov, V. A., additional, Buryakova, A. A., additional, Reverdatto, S. V., additional, Chakhamakhcheva, O. G., additional, Ovchinnikov, Yu. A., additional, Saedler, H., additional, Paz-Ares, J., additional, Wienand, U., additional, Schell, J., additional, Broude, N. E., additional, Sverdlov, E. D., additional, Paves, H., additional, Timmusk, T., additional, Metsis, M., additional, Kelve, M., additional, Toots, U., additional, Neuman, T., additional, Saarma, M., additional, Sakmann, B., additional, Korobko, V. G., additional, Graf, T., additional, Beug, H., additional, Golay, J., additional, Leutz, A., additional, Ness, S., additional, Gruss, P., additional, Gren, E., additional, Borisova, G., additional, Kozlovskaya, T., additional, Pushko, P., additional, Tsibinogin, V., additional, Ose, V., additional, Pumpen, P., additional, Tanyashin, V. I., additional, Georgiev, G. P., additional, Shick, V., additional, Belyavsky, A., additional, Postnikov, Yu., additional, Studitsky, V., additional, Sippel, A. E., additional, Theisen, M., additional, Borgmeyer, U., additional, Rupp, R. A. W., additional, Strech-Jurk, U., additional, Stief, A., additional, Müller, A., additional, Hecht, A., additional, Grussenmeyer, T., additional, Zasedatelev, A. S., additional, Gursky, G. V., additional, Grokhovsky, S. L., additional, Gottikh, B. P., additional, Zhuze, A. L., additional, Hörz, W., additional, Mirzabekov, A., additional, Karpov, V., additional, Preobrazhenskaya, O., additional, Bavykin, S., additional, Ebralidse, K., additional, Vlassov, V. V., additional, Zarytova, V. F., additional, Zachau, H. G., additional, Klobeck, H. G., additional, Lötscher, E., additional, Pargent, W., additional, Pohlenz, H. D., additional, Straubinger, B., additional, Zimmer, F. J., additional, Hofschneider, P. H., additional, Wollersheim, M., additional, Zahm, P., additional, Zabarovsky, E. R., additional, Nurbekov, M. K., additional, Turina, O. V., additional, Kisselev, L. L., additional, Riva, M., additional, Schäffner, A. R., additional, Sentenac, A., additional, Hartmann, G. R., additional, Mustaev, A. A., additional, Zaychikov, E. F., additional, Grachev, M. A., additional, Falkenburg, D., additional, Bautz, E. K. F., additional, Grummt, I., additional, Krämer, A., additional, Frendewey, D., additional, Lahr, G., additional, Christofori, G., additional, Frick, M., additional, and Keller, W., additional

Published
1987
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143. Late Diagnosis of POMC Deficiency and In Vitro Evidence of Residual Translation From Allele With c.-11C>A Mutation.

Author

Anisimova, Aleksandra S, Rubtsov, Petr M, Akulich, Kseniya A, Dmitriev, Sergey E, Frolova, Elena, and Tiulpakov, Anatoly

Abstract

Loss-of-function mutations in the POMC gene are associated with a syndrome with the characteristics of adrenal insufficiency, obesity, and red hair. We describe here a case of pro-opiomelanocortin (POMC) deficiency in which adrenal insufficiency was not treated until the fourth year of life. One of the disease-causative POMC mutations was characterized in vitro using a unique approach.

Published
2017
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149. Evidence for widespread association of mammalian splicing and conserved long-range RNA structures

Author

Pervouchine, Dmitri D., Khrameeva, Ekaterina E., Pichugina, Marina Yu., Nikolaienko, Oleksii V., Gelfand, Mikhail S., Rubtsov, Petr M., and Mironov, Andrei A.

Abstract

Pre-mRNA structure impacts many cellular processes, including splicing in genes associated with disease. The contemporary paradigm of RNA structure prediction is biased toward secondary structures that occur within short ranges of pre-mRNA, although long-range base-pairings are known to be at least as important. Recently, we developed an efficient method for detecting conserved RNA structures on the genome-wide scale, one that does not require multiple sequence alignments and works equally well for the detection of local and long-range base-pairings. Using an enhanced method that detects base-pairings at all possible combinations of splice sites within each gene, we now report RNA structures that could be involved in the regulation of splicing in mammals. Statistically, we demonstrate strong association between the occurrence of conserved RNA structures and alternative splicing, where local RNA structures are generally more frequent at alternative donor splice sites, while long-range structures are more associated with weak alternative acceptor splice sites. As an example, we validated the RNA structure in the human SF1 gene using minigenes in the HEK293 cell line. Point mutations that disrupted the base-pairing of two complementary boxes between exons 9 and 10 of this gene altered the splicing pattern, while the compensatory mutations that reestablished the base-pairing reverted splicing to that of the wild-type. There is statistical evidence for a Dscam-like class of mammalian genes, in which mutually exclusive RNA structures control mutually exclusive alternative splicing. In sum, we propose that long-range base-pairings carry an important, yet unconsidered part of the splicing code, and that, even by modest estimates, there must be thousands of such potentially regulatory structures conserved throughout the evolutionary history of mammals.

Published
2012

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