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103. There's no place like OM: Vesicular sorting and secretion of the peptidylarginine deiminase of Porphyromonas gingivalis

Author

Gabarrini, Giorgio, primary, Palma Medina, Laura M., additional, Stobernack, Tim, additional, Prins, Rianne C., additional, du Teil Espina, Marines, additional, Kuipers, Jeroen, additional, Chlebowicz, Monika A., additional, Rossen, John W. A., additional, van Winkelhoff, Arie Jan, additional, and van Dijl, Jan Maarten, additional

Published
2018
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104. Identification of a Novel Genomic Island Associated with vanD -Type Vancomycin Resistance in Six Dutch Vancomycin-Resistant Enterococcus faecium Isolates

Author

Top, Janetta, primary, Sinnige, Jan C., additional, Brouwer, Ellen C., additional, Werner, Guido, additional, Corander, Jukka, additional, Severin, Juliëtte A., additional, Jansen, Rogier, additional, Bathoorn, E., additional, Bonten, Marc J. M., additional, Rossen, John W. A., additional, and Willems, Rob J. L., additional

Published
2018
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106. Comprehensive Molecular Characterization of Escherichia coli Isolates from Urine Samples of Hospitalized Patients in Rio de Janeiro, Brazil

Author

Campos, Ana Carolina C., primary, Andrade, Nathália L., additional, Ferdous, Mithila, additional, Chlebowicz, Monika A., additional, Santos, Carla C., additional, Correal, Julio C. D., additional, Lo Ten Foe, Jerome R., additional, Rosa, Ana Cláudia P., additional, Damasco, Paulo V., additional, Friedrich, Alex W., additional, and Rossen, John W. A., additional

Published
2018
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107. Metagenomic Characterization of the Human Intestinal Microbiota in Fecal Samples from STEC-Infected Patients

Author

Gigliucci, Federica, primary, von Meijenfeldt, F. A. Bastiaan, additional, Knijn, Arnold, additional, Michelacci, Valeria, additional, Scavia, Gaia, additional, Minelli, Fabio, additional, Dutilh, Bas E., additional, Ahmad, Hamideh M., additional, Raangs, Gerwin C., additional, Friedrich, Alex W., additional, Rossen, John W. A., additional, and Morabito, Stefano, additional

Published
2018
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110. Efficacy of Home Telemonitoring versus Conventional Follow-up: A Randomized Controlled Trial among Teenagers with Inflammatory Bowel Disease

Author

Heida, Anke, primary, Dijkstra, Alie, additional, Muller Kobold, Anneke, additional, Rossen, John W, additional, Kindermann, Angelika, additional, Kokke, Freddy, additional, de Meij, Tim, additional, Norbruis, Obbe, additional, Weersma, Rinse K, additional, Wessels, Margreet, additional, Hummel, Thalia, additional, Escher, Johanna, additional, van Wering, Herbert, additional, Hendriks, Daniëlle, additional, Mearin, Luisa, additional, Groen, Henk, additional, Verkade, Henkjan J, additional, and van Rheenen, Patrick F, additional

Published
2017
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111. Increased risk of pneumonia in residents living near poultry farms : does the upper respiratory tract microbiota play a role?

Author

Smit, Lidwien A M, Boender, Gert Jan, de Steenhuijsen Piters, Wouter A A, Hagenaars, Thomas J, Huijskens, Elisabeth G W, Rossen, John W A, Koopmans, Marion Pg, Nodelijk, Gonnie, Sanders, Elisabeth A M, Yzermans, Joris C, Bogaert, Debby, Heederik, Dick, Smit, Lidwien A M, Boender, Gert Jan, de Steenhuijsen Piters, Wouter A A, Hagenaars, Thomas J, Huijskens, Elisabeth G W, Rossen, John W A, Koopmans, Marion Pg, Nodelijk, Gonnie, Sanders, Elisabeth A M, Yzermans, Joris C, Bogaert, Debby, and Heederik, Dick

Published
2017

112. Increased risk of pneumonia in residents living near poultry farms: does the upper respiratory tract microbiota play a role?

Author

LS IRAS EEPI GRA (Gezh.risico-analyse), dIRAS RA-I&I RA, Smit, L.A., Boender, G.J., de Steenhuijsen Piters, W.A.A., Hagenaars, T.J., Huijskens, E G W, Rossen, John W A, Koopmans, M., Nodelijk, G., Sanders, Elisabeth A M, Yzermans, J., Bogaert, D., Heederik, D., LS IRAS EEPI GRA (Gezh.risico-analyse), dIRAS RA-I&I RA, Smit, L.A., Boender, G.J., de Steenhuijsen Piters, W.A.A., Hagenaars, T.J., Huijskens, E G W, Rossen, John W A, Koopmans, M., Nodelijk, G., Sanders, Elisabeth A M, Yzermans, J., Bogaert, D., and Heederik, D.

Published
2017

113. Increased risk of pneumonia in residents living near poultry farms: does the upper respiratory tract microbiota play a role?

Author

Infectieziekten onderzoek3 (Bogaert), Child Health, Immuno/reuma patientenzorg, Infection & Immunity, Immuno/reuma onderzoek 7 (Montfrans), Smit, Lidwien A M, Boender, Gert Jan, de Steenhuijsen Piters, Wouter A A, Hagenaars, Thomas J, Huijskens, Elisabeth G W, Rossen, John W A, Koopmans, Marion Pg, Nodelijk, Gonnie, Sanders, Elisabeth A M, Yzermans, Joris C, Bogaert, Debby, Heederik, Dick, Infectieziekten onderzoek3 (Bogaert), Child Health, Immuno/reuma patientenzorg, Infection & Immunity, Immuno/reuma onderzoek 7 (Montfrans), Smit, Lidwien A M, Boender, Gert Jan, de Steenhuijsen Piters, Wouter A A, Hagenaars, Thomas J, Huijskens, Elisabeth G W, Rossen, John W A, Koopmans, Marion Pg, Nodelijk, Gonnie, Sanders, Elisabeth A M, Yzermans, Joris C, Bogaert, Debby, and Heederik, Dick

Published
2017

114. Prevalence, risk factors and molecular epidemiology of highly resistant gram negative rods in hospitalized patients in the Dutch region Kennemerland

Author

Souverein, Dennis, Euser, Sjoerd M, Herpers, Bjorn L, Diederen, Bram, Houtman, Patricia, van Seventer, Marina, van Ess, Ingeborg, Kluytmans, Jan, Rossen, John W A, and Den Boer, Jeroen W

Subjects
ESBL, Risk factors, Whole genome sequencing, Prevalence, Journal Article, HR-GNRs, Netherlands
Abstract

BACKGROUND: This paper describes (1) the Highly Resistant Gram Negative Rod (HR-GNR) prevalence rate, (2) their genotypes, acquired resistance genes and (3) associated risk factors of HR-GNR colonization among the hospitalized population in the Dutch region Kennemerland. METHODS: Between 1 October 2013 and 31 March 2014, cross-sectional prevalence measurements were performed in three regional hospitals as part of each hospitals infection control program. Rectal swabs were analyzed at the Regional Public Health Laboratory Kennemerland by direct culturing. Genotypes and acquired resistance genes of positive isolates were determined using Whole Genome Sequencing with the MiSeq instrument (Illumina). Association between several independent variables and HR-GNR positivity was examined using logistic regression models. RESULTS: Out of 427 patients, 24 HR-GNR positive isolates were recovered from 22 patients, resulting in a regional HR-GNR colonization prevalence (95 % CI) of 5.2 % (3.6-7.9). Of these 22 positive patients, 15 were Extended Spectrum Beta-Lactamase (ESBL) positive (3.5 % (2.1-5.7)), 7 patients were positive for a Fluoroquinolones and Aminoglycosides (Q&A) resistant Enterobacteriaceae (1.6 % (0.8-3.3)) and from one patient (0.2 % (0-1.3)) a Stenotrophomonas maltophilia resistant towards co-trimoxazole was isolated. No carbapenemase producing Enterobacteriaceae (CPE), multi-resistant Acinetobacter species or multi-resistant Pseudomonas aeruginosa were isolated. The ESBL genes found were bla CTX-M-1 (n = 4, 25.0 %), bla CTX-M-15 (n = 3, 18.8 %), bla CTX-M-27 (n = 2, 12.5 %), bla CTX-M-14b (n = 2, 12.5 %), bla CTX-M-9 (n = 2, 12.5 %), bla CTX-M-14 (n = 1, 6.3 %), bla CTX-M-3 (n = 1, 6.3 %), bla SHV-11 (n = 1, 6.3 %) and bla SHV-12 (n = 1, 6.3 %). Being known HR-GNR positive in the past was the only significant associated risk factor for HR-GNR positivity, odds ratio (95 % CI): 7.32 (1.82-29.35), p-value = 0.005. CONCLUSIONS: Similar ESBL prevalence rates and genotypes (3.5 %) were found in comparison to other Dutch studies. When previously HR-GNR positive patients are readmitted, they should be screened for HR-GNR colonization since colonization with GR-GNRs could be prolonged. We recommend for future studies to include all defined HR-GNRs in addition to ESBLs in prevalence studies, in order to obtain a more comprehensive overview of colonization with HR-GNRs.

Published
2016

115. Distinguishing blaKPCGene-Containing IncF Plasmids from Epidemiologically Related and Unrelated EnterobacteriaceaeBased on Short- and Long-Read Sequence Data

Author

Stohr, Joep J. J. M., Kluytmans-van den Bergh, Marjolein F. Q., Weterings, Veronica A. T. C., Rossen, John W. A., and Kluytmans, Jan A. J. W.

Abstract

Limited information is available on whether blaKPC-containing plasmids from isolates in a hospital outbreak can be differentiated from epidemiologically unrelated blaKPC-containing plasmids based on sequence data. This study aimed to evaluate the performance of three approaches to distinguish epidemiologically related from unrelated blaKPC-containing pKpQiL-like IncFII(k2)-IncFIB(pQiL) plasmids.

Published
2021
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116. Coronavirus Escape from Heptad Repeat 2 (HR2)-Derived Peptide Entry Inhibition as a Result of Mutations in the HR1 Domain of the Spike Fusion Protein

Author

Bosch, Berend Jan, Rossen, John W A, Bartelink, Willem, Zuurveen, Stephanie J, de Haan, Cornelis A M, Duquerroy, Stephane, Boucher, Charles A B, Rottier, Peter J M, LS Virologie, and LS Virologie

Subjects
Repetitive Sequences, Amino Acid, viruses, Immunology, Coronacrisis-Taverne, MEMBRANE-FUSION, medicine.disease_cause, Membrane Fusion, Microbiology, Repetitive Sequences, Virus, Cell Line, Mice, Viral Envelope Proteins, CLASS-I, Virology, INFECTION, medicine, Animals, Coronaviridae, SUPPRESSION, Coronavirus, SARS, Mutation, Membrane Glycoproteins, biology, GLYCOPROTEIN, Lipid bilayer fusion, IMMUNODEFICIENCY-VIRUS TYPE-1, biology.organism_classification, Fusion protein, Spike Glycoprotein, Virus-Cell Interactions, Entry inhibitor, Amino Acid, Heptad repeat, Insect Science, Spike Glycoprotein, Coronavirus, REPLICATION, HIV-1, SENSITIVITY, Peptides, medicine.drug
Abstract

Peptides based on heptad repeat (HR) domains of class I viral fusion proteins are considered promising antiviral drugs targeting virus cell entry. We have analyzed the evolution of the mouse hepatitis coronavirus during multiple passaging in the presence of an HR2-based fusion inhibitor. Drug-resistant variants emerged as a result of multiple substitutions in the spike fusion protein, notably within a 19-residue segment of the HR1 region. Strikingly, one mutation, an A1006V substitution, which consistently appeared first in four independently passaged viruses, was the main determinant of the resistance phenotype, suggesting that only limited options exist for escape from the inhibitory effect of the HR2 peptide.

Published
2008

119. Methicillin Resistant Staphylococcus aureus Transmission in a Ghanaian Burn Unit: The Importance of Active Surveillance in Resource-Limited Settings

Author

Amissah, Nana Ama, primary, Buultjens, Andrew H., additional, Ablordey, Anthony, additional, van Dam, Lieke, additional, Opoku-Ware, Ampomah, additional, Baines, Sarah L., additional, Bulach, Dieter, additional, Tetteh, Caitlin S., additional, Prah, Isaac, additional, van der Werf, Tjip S., additional, Friedrich, Alexander W., additional, Seemann, Torsten, additional, van Dijl, Jan Maarten, additional, Stienstra, Ymkje, additional, Stinear, Timothy P., additional, and Rossen, John W., additional

Published
2017
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120. Epidemiology of Extended-Spectrum β-Lactamase-Producing E. coli and Vancomycin-Resistant Enterococci in the Northern Dutch–German Cross-Border Region

Author

Zhou, Xuewei, primary, García-Cobos, Silvia, additional, Ruijs, Gijs J. H. M., additional, Kampinga, Greetje A., additional, Arends, Jan P., additional, Borst, Dirk M., additional, Möller, Lieke V., additional, Holman, Nicole D., additional, Schuurs, Theo A., additional, Bruijnesteijn van Coppenraet, Lesla E., additional, Weel, Jan F., additional, van Zeijl, Jan H., additional, Köck, Robin, additional, Rossen, John W. A., additional, and Friedrich, Alexander W., additional

Published
2017
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121. Genetic loci of Staphylococcus aureus associated with anti-neutrophil cytoplasmic autoantibody (ANCA)-associated vasculitides

Author

Glasner, Corinna, primary, de Goffau, Marcus C., additional, van Timmeren, Mirjan M., additional, Schulze, Mirja L., additional, Jansen, Benita, additional, Tavakol, Mehri, additional, van Wamel, Willem J. B., additional, Stegeman, Coen A., additional, Kallenberg, Cees G. M., additional, Arends, Jan P., additional, Rossen, John W., additional, Heeringa, Peter, additional, and van Dijl, Jan Maarten, additional

Published
2017
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124. No nosocomial transmission under standard hygiene precautions in short term contact patients in case of an unexpected ESBL or Q&A E. coli positive patient: a one-year prospective cohort study within three regional hospitals

Author

Souverein, Dennis, primary, Euser, Sjoerd M., additional, Herpers, Bjorn L., additional, Hattink, Corry, additional, Houtman, Patricia, additional, Popma, Amerens, additional, Kluytmans, Jan, additional, Rossen, John W. A., additional, and Den Boer, Jeroen W., additional

Published
2017
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125. Targeted next-generation sequencing of the 16S-23S rRNA region for culture-independent bacterial identification - increased discrimination of closely related species

Author

Sabat, Artur J., primary, van Zanten, Evert, additional, Akkerboom, Viktoria, additional, Wisselink, Guido, additional, van Slochteren, Kees, additional, de Boer, Richard F., additional, Hendrix, Ron, additional, Friedrich, Alexander W., additional, Rossen, John W. A., additional, and Kooistra-Smid, Anna M. D., additional

Published
2017
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127. Increased risk of pneumonia in residents living near poultry farms: does the upper respiratory tract microbiota play a role?

Author

Smit, Lidwien A. M., primary, Boender, Gert Jan, additional, de Steenhuijsen Piters, Wouter A. A., additional, Hagenaars, Thomas J., additional, Huijskens, Elisabeth G. W., additional, Rossen, John W. A., additional, Koopmans, Marion, additional, Nodelijk, Gonnie, additional, Sanders, Elisabeth A. M., additional, Yzermans, Joris, additional, Bogaert, Debby, additional, and Heederik, Dick, additional

Published
2017
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129. Whole-genome multilocus sequence typing of extended-spectrum-beta-lactamase-producing enterobacteriaceae

Author

Kluytmans-Van Den Bergh, Marjolein F Q, Rossen, John W A, Bruijning-Verhagen, Patricia C J, Bonten, Marc J M, Friedrich, Alexander W., Vandenbroucke-Grauls, Christina M J E, Willems, Rob J L, Kluytmans, Jan A.J.W., Kluytmans-Van Den Bergh, Marjolein F Q, Rossen, John W A, Bruijning-Verhagen, Patricia C J, Bonten, Marc J M, Friedrich, Alexander W., Vandenbroucke-Grauls, Christina M J E, Willems, Rob J L, and Kluytmans, Jan A.J.W.

Published
2016

130. Dysbiosis of upper respiratory tract microbiota in elderly pneumonia patients

Author

de Steenhuijsen Piters, Wouter A. A., Huijskens, Elisabeth G. W., Wyllie, Anne L., Biesbroek, Giske, van den Bergh, Menno R., Veenhoven, Reinier H., Wang, Xinhui, Trzcinski, Krzysztof, Bonten, Marc J., Rossen, John W. A., Sanders, Elisabeth A. M., Bogaert, Debby, de Steenhuijsen Piters, Wouter A. A., Huijskens, Elisabeth G. W., Wyllie, Anne L., Biesbroek, Giske, van den Bergh, Menno R., Veenhoven, Reinier H., Wang, Xinhui, Trzcinski, Krzysztof, Bonten, Marc J., Rossen, John W. A., Sanders, Elisabeth A. M., and Bogaert, Debby

Published
2016

131. Prevalence, risk factors and molecular epidemiology of highly resistant gram negative rods in hospitalized patients in the Dutch region Kennemerland

Author

Epi Infectieziekten Team 1, Infection & Immunity, Souverein, Dennis, Euser, Sjoerd M, Herpers, Bjorn L, Diederen, Bram, Houtman, Patricia, van Seventer, Marina, van Ess, Ingeborg, Kluytmans, Jan, Rossen, John W A, Den Boer, Jeroen W, Epi Infectieziekten Team 1, Infection & Immunity, Souverein, Dennis, Euser, Sjoerd M, Herpers, Bjorn L, Diederen, Bram, Houtman, Patricia, van Seventer, Marina, van Ess, Ingeborg, Kluytmans, Jan, Rossen, John W A, and Den Boer, Jeroen W

Published
2016

132. Whole-genome multilocus sequence typing of extended-spectrum-beta-lactamase-producing enterobacteriaceae

Author

Epi Infectieziekten Team 1, Infection & Immunity, Child Health, MMB, Epi Infectieziekten, JC onderzoeksprogramma Infectieziekten, Kluytmans-Van Den Bergh, Marjolein F Q, Rossen, John W A, Bruijning-Verhagen, Patricia C J, Bonten, Marc J M, Friedrich, Alexander W., Vandenbroucke-Grauls, Christina M J E, Willems, Rob J L, Kluytmans, Jan A.J.W., Epi Infectieziekten Team 1, Infection & Immunity, Child Health, MMB, Epi Infectieziekten, JC onderzoeksprogramma Infectieziekten, Kluytmans-Van Den Bergh, Marjolein F Q, Rossen, John W A, Bruijning-Verhagen, Patricia C J, Bonten, Marc J M, Friedrich, Alexander W., Vandenbroucke-Grauls, Christina M J E, Willems, Rob J L, and Kluytmans, Jan A.J.W.

Published
2016

133. Dysbiosis of upper respiratory tract microbiota in elderly pneumonia patients

Author

Infectieziekten onderzoek3 (Bogaert), Child Health, Immuno/reuma onderzoek 8 (Trzcinski), Arts-assistenten Kinderen, Infection & Immunity, Epi Infectieziekten, MMB, JC onderzoeksprogramma Infectieziekten, Immuno/reuma onderzoek 7 (Montfrans), Immuno/reuma patientenzorg, de Steenhuijsen Piters, Wouter A. A., Huijskens, Elisabeth G. W., Wyllie, Anne L., Biesbroek, Giske, van den Bergh, Menno R., Veenhoven, Reinier H., Wang, Xinhui, Trzcinski, Krzysztof, Bonten, Marc J., Rossen, John W. A., Sanders, Elisabeth A. M., Bogaert, Debby, Infectieziekten onderzoek3 (Bogaert), Child Health, Immuno/reuma onderzoek 8 (Trzcinski), Arts-assistenten Kinderen, Infection & Immunity, Epi Infectieziekten, MMB, JC onderzoeksprogramma Infectieziekten, Immuno/reuma onderzoek 7 (Montfrans), Immuno/reuma patientenzorg, de Steenhuijsen Piters, Wouter A. A., Huijskens, Elisabeth G. W., Wyllie, Anne L., Biesbroek, Giske, van den Bergh, Menno R., Veenhoven, Reinier H., Wang, Xinhui, Trzcinski, Krzysztof, Bonten, Marc J., Rossen, John W. A., Sanders, Elisabeth A. M., and Bogaert, Debby

Published
2016

134. Retrospective identification of a previously undetected clinical case of OXA-48-producing K. pneumoniae and E. coli: the importance of adequate detection guidelines

Author

Epi Infectieziekten Team 1, Infection & Immunity, Willemsen, Ina, van Esser, Joost, Kluytmans - van den Bergh, MFQ, Zhou, Kai, Rossen, John W, Verhulst, Carlo, Verduin, Kees, Kluytmans, Jan, Epi Infectieziekten Team 1, Infection & Immunity, Willemsen, Ina, van Esser, Joost, Kluytmans - van den Bergh, MFQ, Zhou, Kai, Rossen, John W, Verhulst, Carlo, Verduin, Kees, and Kluytmans, Jan

Published
2016

138. Prevalence, risk factors and molecular epidemiology of highly resistant gram negative rods in hospitalized patients in the Dutch region Kennemerland

Author

Souverein, Dennis, primary, Euser, Sjoerd M., additional, Herpers, Bjorn L., additional, Diederen, Bram, additional, Houtman, Patricia, additional, van Seventer, Marina, additional, van Ess, Ingeborg, additional, Kluytmans, Jan, additional, Rossen, John W. A., additional, and Den Boer, Jeroen W., additional

Published
2016
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140. Use of whole-genome sequencing to trace, control and characterize the regional expansion of extended-spectrum β-lactamase producing ST15 Klebsiella pneumoniae

Author

Zhou, Kai, primary, Lokate, Mariette, additional, Deurenberg, Ruud H., additional, Tepper, Marga, additional, Arends, Jan P., additional, Raangs, Erwin G. C., additional, Lo-Ten-Foe, Jerome, additional, Grundmann, Hajo, additional, Rossen, John W. A., additional, and Friedrich, Alexander W., additional

Published
2016
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141. Cross-border comparison of the Dutch and German guidelines on multidrug-resistant Gram-negative microorganisms

Author

Müller, Jan, Voss, Andreas, Köck, Robin, Sinha, Bhanu, Rossen, John W, Kaase, Martin, Mielke, Martin, Daniels-Haardt, Inka, Jurke, Annette, Hendrix, Ron, Kluytmans, Jan A, Kluytmans-van den Bergh, Marjolein F, Pulz, Matthias, Herrmann, Jörg, Kern, Winfried V, Wendt, Constanze, Friedrich, Alex W, Müller, Jan, Voss, Andreas, Köck, Robin, Sinha, Bhanu, Rossen, John W, Kaase, Martin, Mielke, Martin, Daniels-Haardt, Inka, Jurke, Annette, Hendrix, Ron, Kluytmans, Jan A, Kluytmans-van den Bergh, Marjolein F, Pulz, Matthias, Herrmann, Jörg, Kern, Winfried V, Wendt, Constanze, and Friedrich, Alex W

Published
2015

142. Cross-border comparison of the Dutch and German guidelines on multidrug-resistant Gram-negative microorganisms

Author

Epi Infectieziekten Team 1, Infection & Immunity, Müller, Jan, Voss, Andreas, Köck, Robin, Sinha, Bhanu, Rossen, John W, Kaase, Martin, Mielke, Martin, Daniels-Haardt, Inka, Jurke, Annette, Hendrix, Ron, Kluytmans, Jan A, Kluytmans-van den Bergh, Marjolein F, Pulz, Matthias, Herrmann, Jörg, Kern, Winfried V, Wendt, Constanze, Friedrich, Alex W, Epi Infectieziekten Team 1, Infection & Immunity, Müller, Jan, Voss, Andreas, Köck, Robin, Sinha, Bhanu, Rossen, John W, Kaase, Martin, Mielke, Martin, Daniels-Haardt, Inka, Jurke, Annette, Hendrix, Ron, Kluytmans, Jan A, Kluytmans-van den Bergh, Marjolein F, Pulz, Matthias, Herrmann, Jörg, Kern, Winfried V, Wendt, Constanze, and Friedrich, Alex W

Published
2015

144. Detection of Bartonella spp. DNA in clinical specimens using an internally controlled real-time PCR assay

Author

Bergmans, Anneke M C, Rossen, John W A, and Microbes in Health and Disease (MHD)

Subjects
DNA, Bacterial, hemic and lymphatic diseases, Cats, bacteria, Animals, Cat-Scratch Disease, Humans, Reference Standards, bacterial infections and mycoses, Bartonella, Environment, Controlled, Real-Time Polymerase Chain Reaction, Specimen Handling
Abstract

Bartonella henselae is the causative agent of cat-scratch disease (CSD), usually presenting itself as a -self-limiting lymphadenopathy. In this chapter an internally controlled Taqman probe-based real-time PCR targeting the groEL gene of Bartonella spp. is described. This assay allows for the rapid, sensitive, and simple detection of Bartonella spp. in samples from CSD or endocarditis suspects, and it is suitable for implementation in the diagnostic microbiology laboratory.

Published
2013

145. Efficacy of Home Telemonitoring versus Conventional Follow-up: A Randomized Controlled Trial among Teenagers with Inflammatory Bowel Disease.

Author

Heida, Anke, Dijkstra, Alie, Kobold, Anneke Muller, Rossen, John W., Kindermann, Angelika, Kokke, Freddy, de Meij, Tim, Norbruis, Obbe, Weersma, Rinse K., Wessels, Margreet, Hummel, Thalia, Escher, Johanna, van Wering, Herbert, Hendriks, Daniëlle, Mearin, Luisa, Groen, Henk, Verkade, Henkjan J., and van Rheenen, Patrick F.

Abstract

Background and Aims: Conventional follow-up of teenagers with inflammatory bowel diseases [IBD] is done during scheduled outpatient visits regardless of how well the patient feels. We designed a telemonitoring strategy for early recognition of flares and compared its efficacy with conventional follow-up. Methods: We used a multicentre randomized trial in patients aged 10-19 years with IBD in clinical remission at baseline. Participants assigned to telemonitoring received automated alerts to complete a symptom score and send a stool sample for measurement of calprotectin. This resulted in an individual prediction for flare with associated treatment advice and test interval. In conventional follow-up the health check interval was left to the physician's discretion. The primary endpoint was cumulative incidence of disease flares. Secondary endpoints were percentage of participants with a positive change in quality-of-life and cost-effectiveness of the intervention. Results: We included 170 participants [84 telemonitoring; 86 conventional follow-up]. At 52 weeks the mean number of face-to-face visits was significantly lower in the telemonitoring group compared to conventional follow-up [3.6 vs 4.3, p < 0.001]. The incidence of flares [33 vs 34%, p = 0.93] and the proportion of participants reporting positive change in quality-of-life [54 vs 44%, p = 0.27] were similar. Mean annual cost-saving was €89 and increased to €360 in those compliant to the protocol. Conclusions: Telemonitoring is as safe as conventional follow-up, and reduces outpatient visits and societal costs. The positive impact on quality-of-life was similar in the two groups. This strategy is attractive for teenagers and families, and health professionals may be interested in using it to keep teenagers who are well out of hospital and ease pressure on overstretched outpatient services. [ABSTRACT FROM AUTHOR]

Published
2018
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146. First report of OXA-48-producing <italic>Escherichia coli</italic> in Croatia and confirmed intergenic transfer of a plasmid-carrying <italic>bla</italic>OXA-48 from <italic>Klebsiella pneumoniae</italic>.

Author

Bošnjak, Zrinka, Chlebowicz, Monika A., Mareković, Ivana, Rezo Vranješ, Violeta, Rossen, John W., and Budimir, Ana

Subjects
ESCHERICHIA coli, KLEBSIELLA pneumoniae, MORPHOLOGY, IMIPENEM, MEROPENEM, COLISTIN
Abstract

The article discusses a study on the emergence of OXA-48-producing Escherichia coli in Denmark and of Klebsiella pneumonia in Hungary. Topics covered include identification of colonies with a typical morphology by standard microbiological testing, determination of the minimum inhibitory concentration (MIC) for imipenem, meropenem and colistin, and key for preventing the spread of resistance.

Published
2018
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147. Evaluation of an Accelerated Workflow for Surveillance of ESBL (CTX-M)-Producing Escherichia coli Using Amplicon-Based Next-Generation Sequencing and Automated Analysis.

Author

Peker, Nilay, Rossen, John W. A., Deurenberg, Ruud H., Langereis, Paula C., Raangs, Erwin G. C., Kluytmans, Jan A., Friedrich, Alexander W., Veenemans, Jacobien, and Sinha, Bhanu

Subjects
DIAGNOSIS of escherichia coli diseases, BETA lactamases, ESCHERICHIA coli proteins, DRUG resistance in bacteria, SENSITIVITY analysis, ESCHERICHIA coli
Abstract

Outbreak management of extended spectrum b-lactamase (ESBL)-producing pathogens requires rapid and accurate diagnosis. However, conventional screening is slow and labor-intensive. The vast majority of the screened samples are negative and detection of non-outbreak-related resistant micro-organisms often complicates outbreak management. In a CTX-M-15-producing Escherichia coli outbreak, 149 fecal samples and rectal eSwabs were collected by a cross-sectional survey in a Dutch nursing home. Samples were processed by routine diagnostic methods. Retrospectively, ESBL-producing bacteria and resistance genes were detected directly from eSwab medium by an accelerated workflow without prior enrichment cultures by an amplicon-based next-generation sequencing (NGS) method, and culture. A total of 27 (18.1%) samples were positive in either test. Sensitivity for CTX-M detection was 96.3% for the phenotypic method and 85.2% for the NGS method, and the specificity was 100% for both methods, as confirmed by micro-array. This resulted in a positive predictive value (PPV) of 100% for both methods, and a negative predictive value (NPV) of 99.2% and 96.8% for the phenotypic method and the NGS method, respectively. Time to result was four days and 14 h for the phenotypic method and the NGS method, respectively. In conclusion, the sensitivity without enrichment shows promising results for further use of amplicon-based NGS for screening during outbreaks. [ABSTRACT FROM AUTHOR]

Published
2018
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148. Characterization of the population structure, drug resistance mechanisms and plasmids of the community-associated Enterobacter cloacae complex in China.

Author

Kai Zhou, Wei Yu, Xiaoli Cao, Ping Shen, Haifeng Lu, Qixia Luo, Rossen, John W. A., Yonghong Xiao, Zhou, Kai, Yu, Wei, Cao, Xiaoli, Shen, Ping, Lu, Haifeng, Luo, Qixia, and Xiao, Yonghong

Subjects
ENTEROBACTER cloacae, DRUG resistance in bacteria, PLASMIDS, REPLICONS, GENETIC overexpression, PUBLIC health, ANTIBIOTICS, BACTERIAL proteins, DRUG resistance in microorganisms, GENES, GRAM-negative bacteria, HYDROLASES, MICROBIAL sensitivity tests, PROTEINS, ENTEROBACTERIACEAE diseases, SEQUENCE analysis, PHARMACODYNAMICS
Abstract

Objectives: To investigate the population structure, drug resistance mechanisms and plasmids of community-associated Enterobacter cloacae complex (CA-ECC) isolates in China.Methods: Sixty-two CA-ECC isolates collected from 31 hospitals across China were typed by hsp60 typing and MLST. ESBL and AmpC-overexpression phenotype was determined by double-disc synergy test. Replicon typing and conjugation were performed for plasmid analysis. All ESBL-positive isolates and representative conjugants were subjected to detailed characterization by WGS.Results: Enterobacter hormaechei and Enterobacter kobei were predominant in our collections. MLST distinguished 46 STs with a polyclonal structure. ST591 was the most prevalent clone detected in northern China. Twenty-two isolates (35.5%) were ESBL positive and half of them were E. kobei. ESBL positivity was related to ESBL production (15/22) and to AmpC overexpression (18/22). Core-genome phylogenetic analysis identified intra- and inter-regional dissemination of ESBL-producing E. kobei clones. ESBL producers were exclusively classified as E. hormaechei and E. kobei, and blaCTX-M-3 was the most prevalent ESBL genotype (10/15) detected in four different environments. In the ESBL-positive population, the ESBL producers encoded more drug resistance genes (8-24 genes) by carrying more plasmids (1-3 plasmids) than the non-ESBL-producing isolates, resulting in an inter-group difference in drug susceptibilities. IncHI-type plasmids were prevalent in the ESBL producers (12/15). All IncHI2-type plasmids (n = 11) carried ESBL genes and shared a similar backbone to p09-036813-1A_261 recovered from Salmonella enterica in Canada.Conclusions: The species-specific distribution, species-dependent ESBL mechanism and endemic plasmids identified in our study highlight the necessity for tailored surveillance of CA-ECC in the future. [ABSTRACT FROM AUTHOR]

Published
2018
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149. Impact of PCR for respiratory viruses on antibiotic use: Theory and practice

Author

van de Pol, Alma C., Wolfs, Tom F. W., Tacke, Carline E. A., Uiterwaal, Cuno S. P., Forster, Johannes, van Loon, Anton M., Kimpen, Jan L. L., Rossen, John W. A., and Jansen, Nicolaas J. G.

Subjects
INFLUENZA-VIRUS, patient care management, YOUNG-CHILDREN, PARAINFLUENZA VIRUSES, POLYMERASE-CHAIN-REACTION, RAPID DIAGNOSIS, SYNCYTIAL VIRUS, fungi, real-time polymerase chain reaction, questionnaires, pediatric intensive care units, CONTROLLED-TRIAL, TRACT INFECTIONS, EMERGENCY-DEPARTMENT, anti-bacterial agents, REAL-TIME PCR, viral lower respiratory tract infections
Abstract

RATIONALE FOR THE STUDY: Real-time polymerase chain reaction (PCR) for respiratory viruses is more sensitive, yet more expensive, than conventionally used direct immunofluorescence (DIF). We determined the impact of real-time PCR, additional to DIF, on antibiotic prescription in ventilated children with lower respiratory tract infection (LRTI) at admission to the pediatric intensive care unit (PICU). METHODS: First, a multicenter survey study was performed. Subsequently, in a prospective study, children (≤5 years) with LRTI were tested at admission by DIF and PCR. Positive DIF results were reported at the end of the first working day. PICU physicians reported antibiotic treatment on the second working day. After informing them of the PCR result antibiotic treatment was reevaluated. RESULTS: The multicenter survey study (94 respondents) showed that PCR decreased antibiotic use (P

Published
2011

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