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108 results on '"Rosario Muleo"'

104. Chloroplast DNA polymorphisms as molecular markers to identify cultivars of Olea europaea L

Author

Marcello Buiatti, M.C. Intrieri, and Rosario Muleo

Subjects
Germplasm, Genetics, biology, food and beverages, Horticulture, biology.organism_classification, DNA extraction, Chloroplast DNA, Olea, Genetic marker, Cultivar, Applications of PCR, Genotyping
Abstract

SummaryThe genotyping of cultivated varieties using molecular markers is a crucial aim of modern plant breeding programmes, germplasm collection management and product traceability. Methods for DNA extraction from olive oil are now available, and the DNA extracted is suitable for PCR applications. The aim of this work was to identify simple and reliable PCR markers useful for cultivar identification and oil traceability. We chose to analyse 13 cultivars and one feral accession of Olea europaea for polymorphism in the intergenic spacer of chloroplast DNA (cpDNA). The spacer analysed in this work showed a pattern of variation that was sufficient to discriminate four out of the 13 cultivars analysed. We propose an identification protocol for these cultivars based on the amplification and subsequent sequencing of the chloroplast trnT-trnD intergenic spacer. The ability to design primers pairs capable of amplifying very small fragments makes these markers useful in traceability protocols, even where the qualit...

107. Genetic Mapping and Detection of Quantitative Trait Loci

Author

Luciana Baldoni, Bouchaib Khadari, Raúl de la Rosa, Institute of Biosciences and Bioresources, Consiglio Nazionale delle Ricerche [Roma] (CNR), Amélioration génétique et adaptation des plantes méditerranéennes et tropicales (UMR AGAP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), IFAPA Centro Alameda del Obispo, Instituto Andaluz de Investigación y Formación Agraria y Pesquera (IFAPA), Eddo Rugini, Luciana Baldoni, Rosario Muleo, Luca Sebastiani, Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro), Centro 'Alameda del Obispo', Andalusian Agricultural Research Institute (IFAPA), Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)

Subjects
0106 biological sciences, cartographie de qtl, carte génétique, Genome-wide association study, Biology, Quantitative trait locus, 01 natural sciences, research networks, 03 medical and health sciences, olivier, réseau de recherche, Gene mapping, Family-based QTL mapping, GWAS, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, Association mapping, 030304 developmental biology, Genetic association, amélioration génétique, 2. Zero hunger, 0303 health sciences, qtl, food and beverages, 15. Life on land, Cross-progeny, pays méditerranéen, Genetic architecture, Evolutionary biology, genetic mapping, 010606 plant biology & botany
Abstract

UMR AGAP - équipe AFEF - Architecture et fonctionnement des espèces fruitières; Olive tree is a long-living woody species with similar genomic and phenotypic constraints to other perennial fruit crops. However, compared to apple, grape, and peach, genomic investigations for designing innovative breeding strategies are still limited to only preliminary research in this species. In this chapter, we aim to describe the studies on genetic mapping and underline the most promising investigations and initiatives to build a Mediterranean network suitable for establishing robust marker-trait associations through QTL mapping and association studies. These tools should serve to finally implement new breeding programs driven by marker-assisted breeding.

Published
2016

108. Olive Genetic Resources

Author

Melek Gurbuz Veral, Roberto Mariotti, Luciana Baldoni, Hassane Sikaoui, Bouchaib Khadari, Abdelmajid Moukhli, Angjelina Belaj, IFAPA Centro Alameda del Obispo, Instituto Andaluz de Investigación y Formación Agraria y Pesquera (IFAPA), Olive Research Institute, UR Amélioration des Plantes, Institut National de la Recherche Agronomique (INRA), Amélioration génétique et adaptation des plantes méditerranéennes et tropicales (UMR AGAP), Institut National de la Recherche Agronomique (INRA)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Institute of Biosciences and Bioresources, Consiglio Nazionale delle Ricerche [Roma] (CNR), Eddo Rugini, Luciana Baldoni, Rosario Muleo, Luca Sebastiani, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Centro 'Alameda Del Obispo', Andalusian Institute of Agricultural and Fisheries Research and Training (IFAPA), and Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)-Institut National de la Recherche Agronomique (INRA)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)

Subjects
0106 biological sciences, 0301 basic medicine, Germplasm, Collection, Cultivars, media_common.quotation_subject, Horticultural crops, Biology, 01 natural sciences, Mediterranean Basin, Genetic diversity, bassin méditerranéen, 03 medical and health sciences, olivier, Genetic resources, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, media_common, 2. Zero hunger, Unusual case, Ecology, Longevity, 15. Life on land, espèce sauvage, genêtic variation, Wild olives, 030104 developmental biology, diversité génétique, wild species, conservation des ressources génétiques, Species richness, germplasme, 010606 plant biology & botany, Diversity (politics)
Abstract

UMR AGAP - équipe AFEF - Architecture et fonctionnement des espèces fruitières; As one of the most important and ancient fruit crops in the Mediterranean Basin, olive is characterized by a huge genetic patrimony, represented by cultivated and wild germplasm, ancient trees and related forms. The richness of this germplasm represents an unusual case among horticultural crops, due to species longevity, lack of new better performing genotypes, and the millennial tradition of cultivation. Focusing on a wide spectrum of genetic resources, their conservation, characterization, and management, this chapter tries to give an insight into the achievements and the necessities of this type of works in olive. Knowledge of existing diversity among the olive genetic resources is essential to maximize their conservation, safeguard, and exploitation.

Published
2016

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