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102. Histone/protamine content of frozen bull sperm cells

Author

Le Danvic, Chrystelle, Bray, Fabrice, Sellem, Eli, Kiefer, Hélène, Jammes, Hélène, Rolando, Christian, Schibler, Laurent, Allice, Miniaturisation pour la Synthèse, l’Analyse et la Protéomique - USR 3290 (MSAP), Institut de Chimie du CNRS (INC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Biologie du Développement et Reproduction (BDR), École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA), Université Paris Saclay (COmUE), ANR (LabCom SeQuamol), International Organisation on Animal Reproduction. FRA., ProdInra, Migration, Miniaturisation pour la Synthèse, l’Analyse et la Protéomique - UAR 3290 (MSAP), Université de Lille-Centre National de la Recherche Scientifique (CNRS), and École nationale vétérinaire - Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)

Subjects
[SDV.BDD] Life Sciences [q-bio]/Development Biology, [SDV.BDLR]Life Sciences [q-bio]/Reproductive Biology, [SDV.BDD]Life Sciences [q-bio]/Development Biology, [SDV.BDLR] Life Sciences [q-bio]/Reproductive Biology, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2016

103. Gold nanoparticle synthesis in microfluidic systems and immobilisation in microreactors designed for the catalysis of fine organic reactions

Author

Rolando Christian, Payen Edmond, Ftouni Jamal, Girardon Jean-Sébastien, and Penhoat Maël

Subjects
Materials science, Microfluidics, Nanoparticle, Nanotechnology, Condensed Matter Physics, Chemical reaction, Electronic, Optical and Magnetic Materials, Catalysis, Organic reaction, Hardware and Architecture, Colloidal gold, Surface modification, Electrical and Electronic Engineering, Microreactor
Abstract

Our work is focused on two applications of fine tunable microfluidic systems, first to optimize heterogeneous size nanoparticle synthesis and second to build catalytic microreactors for advanced organic reactions. The first part of our work consists in the use of an original microfluidic setup for gold nanoparticle synthesis, which allows a high control of the reaction parameters as the reactants flow, the concentration, the temperature, and the reaction time. We show that using such microfluidic systems permit a better control of the reaction parameters for producing homodispersed 1–2 nm gold nanoparticle. The second part of our work deals with the incorporation of gold nanoparticles into silica capillaries to build catalytic microreactors dedicated to fine chemical reactions. Our strategy consists in the immobilization of gold nanopadiegolirticles onto the inner surface (2D dispersion) or into the inner volume (3D dispersion) of functionalized silica microcapillaries. Characterizations show that by different functionalization procedures, those gold nanoparticles are well anchored inside the microcapillary.

Published
2011
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105. Polymer phase transition in n-lauryl methacrylate monoliths

Author

Dembahri, Zahra, le Gac, Severine, Tobal, Kamal, Chirani, Naziha, Rolando, Christian, Benmouna, Farida, and Benmouna, Mustapha

Subjects
IR-103624, METIS-321611
Abstract

Monolithic materials prepared from a mixture of n-lauryl methacrylate (LMA) and ethylene glycol dimethacrylate (EGDMA) dedicated to nano-liquid chromatography separation were synthesized using in situ UV polymerization in 75 µm inner diameter capillary tubing. A mixture of cyclohexanol and ethylene glycol was used as a porogen to control porosity. While the preparation conditions yielded satisfactory analytical results, values of pertinent parameters turned out to be critical for obtaining columns with efficient separation. In particular, the impact of two key parameters was studied here in an attempt to identify optimal preparation conditions: (a) different concentrations of the crosslinker EGDMA and (b) different porogen compositions while the monomer to porogen ratio was kept constant. Resulting monolithic phases were characterized in terms of permeability, mean pore diameter and swelling using three different eluents (water, acetonitrile and a mixture at maximum viscosity). First, the LMA/EGDMA monolithic phases present peculiar morphology and hydrodynamic properties for 37% by weight of EGDMA, as reflected by the peak observed for their permeability and mean pore diameter. Swelling experiments revealed the coexistence of two phases in the monolithic structure: a highly crosslinked rigid phase which was insensitive to swelling in the presence of solvent and a weakly crosslinked flexible phase exhibiting significant swelling, with a transition to such a biphasic behavior taking place at 37% by weight of EGDMA. The effects of porogen composition and network swelling properties are presented based on a combination of the Flory − Huggins theory of isotropic mixing in polymer solutions and the Flory − Rehner theory of rubber elasticity in the affine network approximation. © 2016 Society of Chemical Industry

Published
2016

110. Free radical chemistry of flavan-3-ols: determination of thermodynamic parameters and of kinetic reactivity from short(ns) to long(ms) time scale

Author

Cren-Olive, Cecila, Hapiot, Philippe, Pinson, Jean, and Rolando, Christian

Subjects
Thermodynamics -- Research, Chemistry, Physical and theoretical -- Research, Flavones -- Chemical properties, Flavonoids -- Chemical properties, Bioflavonoids -- Chemical properties, Chemistry
Abstract

The physical chemistry and the free radical chemistry of flavan-3-ols are extensively investigated by laser flash photolysis and cyclic voltammetry studies. The results are very useful in discussing biological and biochemical phenomenon and give a new insight into the antioxidative properties of flavan-3-ols.

Published
2002

111. Comparative Proteomic and Transcriptomic Analysis of Follistatin-Induced Skeletal Muscle Hypertrophy.

Author

UCL - SSS/IREC/EDIN - Pôle d'endocrinologie, diabète et nutrition, UCL - (SLuc) Service d'endocrinologie et de nutrition, Barbé, Caroline, Bray, Fabrice, Gueugneau, Marine, Devassine, Stéphanie, Lause, Pascale, Tokarski, Caroline, Rolando, Christian, Thissen, Jean-Paul, UCL - SSS/IREC/EDIN - Pôle d'endocrinologie, diabète et nutrition, UCL - (SLuc) Service d'endocrinologie et de nutrition, Barbé, Caroline, Bray, Fabrice, Gueugneau, Marine, Devassine, Stéphanie, Lause, Pascale, Tokarski, Caroline, Rolando, Christian, and Thissen, Jean-Paul

Abstract

Skeletal muscle, the most abundant body tissue, plays vital roles in locomotion and metabolism. Myostatin is a negative regulator of skeletal muscle mass. In addition to increasing muscle mass, Myostatin inhibition impacts muscle contractility and energy metabolism. To decipher the mechanisms of action of the Myostatin inhibitors, we used proteomic and transcriptomic approaches to investigate the changes induced in skeletal muscles of transgenic mice overexpressing Follistatin, a physiological Myostatin inhibitor. Our proteomic workflow included a fractionation step to identify weakly expressed proteins and a comparison of fast versus slow muscles. Functional annotation of altered proteins supports the phenotypic changes induced by Myostatin inhibition, including modifications in energy metabolism, fiber type, insulin and calcium signaling, as well as membrane repair and regeneration. Less than 10% of the differentially expressed proteins were found to be also regulated at the mRNA level but the Biological Process annotation, and the KEGG pathways analysis of transcriptomic results shows a great concordance with the proteomic data. Thus this study describes the most extensive omics analysis of muscle overexpressing Follistatin, providing molecular-level insights to explain the observed muscle phenotypic changes.

Published
2017

112. Formulation and characterization of quercetin-loaded Prunus armeniacagum nanoparticles with enhanced anti-bacterial effect

Author

Mazyed, Eman A., Magdy, Galal, Elekhnawy, Engy, Yammine, Marie, Rolando, Christian, and ElNaggar, Mai H.

Abstract

Owing to the dissemination of antibiotic resistance among Staphylococcus aureusbacteria, it is critical to find out new, safe, and effective alternatives. Plants are a major source of many bioactive compounds that have a wide range of pharmacological activities. Quercetin is an efficient plant flavonoid that has many pharmacological effects such as the anti-microbial potential. Unfortunately, its applicability is limited due to its poor solubility, limited bioavailability and instability. Nano-drug delivery could improve efficiently drug solubility and bioavailability. The inclusion of natural components in the manufacture of nano-carriers improves their safety and biocompatibility. The aim of the present study is the fabrication of green nanoparticles of the natural bioactive, quercetin, using natural Prunus armeniacagum exudate (PAGE) as a nano-carrier. The gum composition was characterized by a glycoproteomic study which revealed the presence of polysaccharide elements from the arabinogalactan family. It also indicated chemical modifications of the gum by oxidation and the existence of many proteins involved in the plant's defense against environmental stress. The development of quercetin-loaded PAGE-NPs was based on the electrostatic interactions of calcium ions with PAGE. The formulation process was based on 23factorial design. After examining how the independent factors significantly affected drug encapsulation efficiency (DEE%) and % quercetin released after 24 h (Q24h), F6 was chosen as the optimized PAGE-NPs. F6 showed significant improvement in the intestinal permeation and stability than the unprocessed quercetin. Regarding the anti-bacterial effect, there was an improvement in the histological features in the PAGE NPs-treated group. Also, there was a significant decline in the bacterial load and enhancement of the survival rate in this group. In addition, the inflammatory mediators (IL-1β and IL-6) substantially diminished in PAGE NPs-treated group. Consequently, PAGE-NPs are efficient green nano-drug delivery systems for enhancing the pharmacological effect of quercetin.

Published
2024
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119. Comparative Proteomic and Transcriptomic Analysis of Follistatin-Induced Skeletal Muscle Hypertrophy

Author

Barbé, Caroline, Bray, Fabrice, Gueugneau, Marine, Devassine, Stéphanie, Lause, Pascale, Tokarski, Caroline, Rolando, Christian, and Thissen, Jean-Paul

Abstract

Skeletal muscle, the most abundant body tissue, plays vital roles in locomotion and metabolism. Myostatin is a negative regulator of skeletal muscle mass. In addition to increasing muscle mass, Myostatin inhibition impacts muscle contractility and energy metabolism. To decipher the mechanisms of action of the Myostatin inhibitors, we used proteomic and transcriptomic approaches to investigate the changes induced in skeletal muscles of transgenic mice overexpressing Follistatin, a physiological Myostatin inhibitor. Our proteomic workflow included a fractionation step to identify weakly expressed proteins and a comparison of fast versus slow muscles. Functional annotation of altered proteins supports the phenotypic changes induced by Myostatin inhibition, including modifications in energy metabolism, fiber type, insulin and calcium signaling, as well as membrane repair and regeneration. Less than 10% of the differentially expressed proteins were found to be also regulated at the mRNA level but the Biological Process annotation, and the KEGG pathways analysis of transcriptomic results shows a great concordance with the proteomic data. Thus this study describes the most extensive omics analysis of muscle overexpressing Follistatin, providing molecular-level insights to explain the observed muscle phenotypic changes.

Published
2024
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120. Scale-up Study of Benzoic Acid Alkylation in Flow: From Microflow Capillary Reactor to a Milliflow Reactor

Author

Penverne, Christophe, Hazard, Benjamin, Rolando, Christian, and Penhoat, Maël

Abstract

The esterification reaction is one of the most important reaction in organic chemistry, and the development of scalable continuous flow conditions is of high interest. Here we describe a scaling-up study of benzoic acid alkylation by MeI in the presence of superbases from a capillary reactor (282 mg·h–1at 75 °C) to a low flow reactor (LFR, 6.82 g·h–1at 80 °C). First, the optimization of the base demonstrated that 1,8-diazabicyclo[5.4.0]-undec-7-ene (DBU) was a good compromise in terms of basicity and price. A comparison between batch and LFR reaction showed similar kinetic rates. We also observed a maximum reactivity for a flow rate of 3 mL·min–1which corresponds to an optimum mixing of the entering fluids. A colorimetric study permits to show a visual change in the flow regime (dispersed to stratified) by varying the flow rate with a maximum conversion obtained at 3 mL·min–1corresponding to a 105 s residence time, demonstrating that the studied reaction is sensitive to mixing conditions and that LFR presents some mixing limitations for low flow rates. After thermal optimization (80 °C), the reaction has been improved to a productivity of 6.82 g/h at the lab scale using DBU as the base and Eyring/Arrhenius plots permitted to extract activation energies.

Published
2024
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121. Newly designed polyacrylamide/dextran gels for electrophoresis protein separation: synthesis and characterization

Author

AINSEBA-CHIRANI, Naziha, DEMBAHRI, Zahra, TOKARSKI, Caroline, ROLANDO, Christian, and BENMOUNA, Mustapha

Subjects
swelling, absorbance, polyacrylamide/dextran gels, Duracryl™, SDS-PAGE
Abstract

Newly designed gels for electrophoresis protein separation were synthesized from acrylamide, N,N′-methylenebis (acrylamide) and dextran mixtures. Radical polymerization was initiated by ammonium persulfate and N,N,N′,N′-tetramethylethylenediamine. The time dependence of absorbance during polymerization was monitored using UV-visible spectroscopy. The exothermic polymerization process exhibited a sharp rise of temperature reminiscent of the Trommsdorff effect. The swelling kinetics of the synthesized gels was examined in deionized water and buffer solutions. One of the challenges was to find an alternative to commercial products, sold as mixtures with no detailed chemical contents, commonly used in sodium dodecylsulfate polyacrylamide gel electrophoresis (SDS-PAGE) for protein separation. For this reason, a systematic comparison was made of the properties of one of the most commonly used commercial gels, Duracryl™ from Genomics Solution Inc., and those of the synthesized polyacrylamide/dextran gels. POLYMER INTERNATIONAL, ISSN : 0959-8103, DOI : 10.1002/pi.3035, Issue : 7, Volume : 60, pp. 1024– 1029, JUILLET 2011.

Published
2011

126. Microfabricated Nanoelectrospray Emitter Tips based on a Microfluidic Capillary Slot (Chapter 5)

Author

Arscott, Steve, Rolando, Christian, le Gac, Severine, le Gac, S., van den Berg, A., and van den Berg, Albert

Subjects
IR-69346, METIS-263710, EWI-14758
Abstract

An impetus in the field of mass spectrometry (MS) analysis occurred in the early 1990s with the invention of two novel and 'soft' ionization methods, electrospray ionization (ESI) by John Fenn and matrix-assisted laser desorption ionization (MALDI) by Koichi Tanaka, who both shared the Nobel-prize in Chemistry in 2002. http://www.springer.com/chemistry/analytical/book/978-0-85404-129-9

Published
2009

129. Microfabricated Nanoelectrospray Emitter Tips based on a Microfluidic Capillary Slot [Chapter 5]

Author

Le Gac, Séverine, Arscott, S., Rolando, Christian, Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), Miniaturisation pour la Synthèse, l’Analyse et la Protéomique - UAR 3290 (MSAP), Université de Lille-Centre National de la Recherche Scientifique (CNRS), and Le Gac S., Van den Berg A.

Subjects
[SPI]Engineering Sciences [physics], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2008
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130. Mise en place de nouvelles stratégies analytiques pour la sauvegarde des informations chimiques conservées dans des céramiques archéologiques

Author

Regert , Martine, Mirabaud , Sigrid, Pétrequin , Pierre, Pétrequin , Anne-Marie, Rolando , Christian, Centre d'Études Préhistoire, Antiquité, Moyen-Age ( CEPAM ), Université Nice Sophia Antipolis ( UNS ), Université Côte d'Azur ( UCA ) -Université Côte d'Azur ( UCA ) -Centre National de la Recherche Scientifique ( CNRS ), Centre de Recherche et de Restauration des Musées de France, Palais du Louvre, Laboratoire Chrono-environnement ( LCE ), Université Bourgogne Franche-Comté ( UBFC ) -Centre National de la Recherche Scientifique ( CNRS ) -Université de Franche-Comté ( UFC ), Maison des Sciences de l'Homme et de l'Environnement Claude Nicolas Ledoux ( MSHE ), Centre National de la Recherche Scientifique ( CNRS ) -Université de Franche-Comté ( UFC ), Centre d'Études Préhistoire, Antiquité, Moyen-Age (CEPAM), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS), Centre de recherche et de restauration des musées de France (C2RMF), Centre National de la Recherche Scientifique (CNRS)-Ministère de la Culture et de la Communication (MCC), Laboratoire Chrono-environnement - CNRS - UBFC (UMR 6249) (LCE), Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC), Maison des Sciences de l'Homme et de l'Environnement Claude Nicolas Ledoux (UAR 3124) (MSHE), Université de Franche-Comté (UFC), Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)-Centre National de la Recherche Scientifique (CNRS), and Maison des Sciences de l'Homme et de l'Environnement Claude Nicolas Ledoux (MSHE)

Subjects
Céramiques, Analyses, [SHS.ARCHEO]Humanities and Social Sciences/Archaeology and Prehistory, [ SHS.ARCHEO ] Humanities and Social Sciences/Archaeology and Prehistory, Néolithique, Résidus organiques, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2008

132. A Preliminary Work on Evolutionary Identification of Protein Variants and New Proteins on Grids

Author

Boisson, Jean-Charles, Jourdan, Laetitia, Talbi, El-Ghazali, Rolando, Christian, Laboratoire d'Informatique Fondamentale de Lille (LIFL), Université de Lille, Sciences et Technologies-Institut National de Recherche en Informatique et en Automatique (Inria)-Université de Lille, Sciences Humaines et Sociales-Centre National de la Recherche Scientifique (CNRS), Parallel Cooperative Multi-criteria Optimization (DOLPHIN), Université de Lille, Sciences et Technologies-Institut National de Recherche en Informatique et en Automatique (Inria)-Université de Lille, Sciences Humaines et Sociales-Centre National de la Recherche Scientifique (CNRS)-Université de Lille, Sciences et Technologies-Institut National de Recherche en Informatique et en Automatique (Inria)-Université de Lille, Sciences Humaines et Sociales-Centre National de la Recherche Scientifique (CNRS)-Inria Lille - Nord Europe, Institut National de Recherche en Informatique et en Automatique (Inria), Laboratoire de Chimie Organique et Macromoleculaire (UMR CNRS 8009), Université de Lille, Sciences et Technologies-Ecole Nationale Supérieure de Chimie de Lille (ENSCL), and Grid'5000

Subjects
Proteomics, de novo, protein identification, [INFO.INFO-BI]Computer Science [cs]/Bioinformatics [q-bio.QM], [SDV.BIBS]Life Sciences [q-bio]/Quantitative Methods [q-bio.QM], mass spectrometry
Abstract

International audience; Protein identification is one of the major task of Proteomics researchers. Protein identification could be resumed by searching the best match between an experimental mass spectrum and proteins from a database. Nevertheless this approach can not be used to identify new proteins or protein variants. In this paper an evolutionary approach is proposed to discover new proteins or protein variants thanks a "de novo sequencing" method. This approach has been experimented on a specific grid called Grid5000 with simulated spectra and also real spectra.

Published
2006

133. Microfluidique intégrée pour le couplage d’un microcanal à une interface nano-esi : application à la protéomique

Author

Arscott, S., Rolando, Christian, Le Gac, Séverine, Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Institut supérieur de l'électronique et du numérique (ISEN)-Université de Valenciennes et du Hainaut-Cambrésis (UVHC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF), Laboratoire de Chimie Organique et Macromoleculaire (UMR CNRS 8009), and Université de Lille, Sciences et Technologies-Ecole Nationale Supérieure de Chimie de Lille (ENSCL)

Subjects
[SPI]Engineering Sciences [physics], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2006

134. A la recherche des espèces messicoles des pelouses calcicoles de la réserve naturelle des vallées de la Grand-Pierre et de Vitain (Loir-et-Cher)

Author

Forey, I., Dutoit, Thierry, Bézannier, F., Maubert, P., Rolando, Christian, ProdInra, Migration, Unité mixte de recherche Ecologie des invertébrés (UAPV), and Institut National de la Recherche Agronomique (INRA)-Avignon Université (AU)

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], MESSICOLE, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2005

142. Isotopic labelling of proanthocyanidins

Author

Deprez, S., Buffnoir, S., Scalbert, Augustin, Rolando, Christian, ProdInra, Migration, Chimie Biologique (UCB), Institut National de la Recherche Agronomique (INRA)-Institut National Agronomique Paris-Grignon (INA P-G), Sécurité et Qualité des Produits d'Origine Végétale (SQPOV), and Avignon Université (AU)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)

Subjects
[SDV] Life Sciences [q-bio], [SPI.GPROC] Engineering Sciences [physics]/Chemical and Process Engineering, [SDV]Life Sciences [q-bio], [SDV.IDA]Life Sciences [q-bio]/Food engineering, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, [SDV.IDA] Life Sciences [q-bio]/Food engineering, TANNIN, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
1997

143. Electrochemical and radiolytic oxidation of naturally occuring phenols. The combined use of electrochemical and radiolytic techniques permits elucidation of the mechanism of oxidation of phenols and characterization of the intermediates

Author

FULCRAND, Hélène, Hapiot, P., Neta, P., Pinson, J., Rolando, Christian, Laboratoire des polymères et des techniques physicochimiques, Institut National de la Recherche Agronomique (INRA), and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
1997

144. Analysis of fatty acids in lamb samples by GC-MS

Author

Zang, R., Charbonnier, Fabien, Rolando, Christian, Bas, Pierre, Arpino, P., Physiologie de la Nutrition et Alimentation (PNA), Institut National Agronomique Paris-Grignon (INA P-G)-Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique (INRA), and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], [INFO] Computer Science [cs]
Abstract

1 tables 5 graph.; National audience

Published
1997

145. New methods for analysing old plant polyphenols. Dossier dedicated to Professor Edwin Haslam on the occasion of his 65th birthday

Author

Scalbert, Augustin, Rolando, Christian, ProdInra, Migration, Sécurité et Qualité des Produits d'Origine Végétale (SQPOV), and Avignon Université (AU)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)

Subjects
[SDV] Life Sciences [q-bio], [SPI.GPROC] Engineering Sciences [physics]/Chemical and Process Engineering, [SDV]Life Sciences [q-bio], [SDV.IDA]Life Sciences [q-bio]/Food engineering, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, [SDV.IDA] Life Sciences [q-bio]/Food engineering, TANNIN, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
1997

147. Archéologie des résidus organiques : de la chimie analytique à l'archéologie, un état de la question

Author

Regert, Martine, Rolando, Christian, Centre d'Études Préhistoire, Antiquité, Moyen-Age (CEPAM), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Chimie Organique et Macromoleculaire (UMR CNRS 8009), and Université de Lille, Sciences et Technologies-Ecole Nationale Supérieure de Chimie de Lille (ENSCL)

Subjects
Biomarqueurs, Archéologie, [SHS.ARCHEO]Humanities and Social Sciences/Archaeology and Prehistory, Brai de bouleau, Résidus organiques, Analyse, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
1996

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