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Poultry meat has been a vehicle of antibiotic resistant bacteria and genes. Yet, the diversity of selective pressures associated with their maintenance in the poultry-production chain remains poorly explored. We evaluated the susceptibility of Enterococcus spp. from chicken meat collected 20 years apart to antibiotics, metals, acidic pH and peracetic acid-PAA. Contemporary chicken-meat samples (n = 53 batches, each including a pool of neck skin from 10 single carcasses) were collected in a slaughterhouse facility using PAA as disinfectant (March-August 2018, North of Portugal). Broilers were raised in intensive farms (n = 29) using CuSO4 and organic acids as feed additives. Data were compared with that of 67 samples recovered in the same region during 1999-2001. All 2018 samples had multidrug resistant-MDR isolates, with >45 % carrying Enterococcus faecalis, Enterococcus faecium or Enterococcus gallinarum resistant to tetracycline, erythromycin, ampicillin, quinupristin-dalfopristin, ciprofloxa-cin, chloramphenicol or aminoglycosides. Resistance rates were similar (P > 0.05) to those of 1999-2001 samples for all but five antibiotics. The decrease of samples carrying vancomycin-resistant isolates from 46 % to % between 1999-2001 and 2018 was the most striking difference. Isolates from both periods were similarly susceptible to acid pH [minimum-growth pH (4.5-5.0), minimum-survival pH (3.0-4.0)] and to PAA (MIC90 = 100-120 mg/L/MBC90 = 140-160 mg/L; below concentrations used in slaughterhouse). Copper tolerance genes (tcrB and/or cueO) were respectively detected in 21 % and 4 % of 2018 and 1999-2001 samples. The tcrB gene was only detected in E. faecalis (MICCuSO4 > 12 mM), and their genomes were compared with other international ones of chicken origin (PATRIC database), revealing a polyclonal population and a plasmid or chromosomal location for tcrB. The tcrB plasmids shared diverse genetic modules, including multiple antimicrobial resistance genes (e.g. to tetracyclines, chloramphenicol, macrolide-lincosamide-streptogramin B-MLSB, aminoglycosides, bacitracin, coccidiostats). When in chromosome, the tcrB gene was co-located closely to merA (mercury) genes. Chicken meat remains an important vehicle of MDR Enterococcus spp. able to survive under diverse stresses (e.g. copper, acid) potentially contributing to these bacteria maintenance and flux among animal-environment -humans.

The present document describes the HUG project data management plan (DMP), which is neededto specify what data is being processed and to manage how that data will be handled, stored and shared. Funded by the Portuguese Foundation for Science and Technology (PTDC/GES-OUT/1662/2020), this project is expected to generate evidence on the health consequences of the neighbourhood social changes caused by the housing crisis to inform the design and planning of healthier neighbourhoods and housing conditions. More information about the project can be found at:https://www.hug-project.com/ Funding statement:This project is financed by national funds through FCT- Fundação para a Ciência e a Tecnologia, I.P., under the project “HUG: The health impacts of inner-city gentrification, displacement and housing insecurity: a quasi-experimental multi-cohort study (PTDC/GES-OUT/1662/2020)” and the projects UIDB/04750/2020 and LA/P/0064/2020.Ana Isabel Ribeiro, the project's principal investigator, is supported by National Funds through FCT, under the ‘Stimulus of Scientific Employment–Individual Support’ programme within the contract CEECIND/02386/2018.

Background: Antimicrobial resistance (AMR) is one of the main threats to global health. The dissemination of mobile colistin resistance genes (mcr) is a major concern affecting humans, animals and environment. Food-producing animals are considered the main reservoir of mcr genes, however the role of wild animals and pets is less studied. Here, we investigate the mcr occurrence among wild animals, wild birds, food-producing animals and pets. Material & Methods: Between 2019-2021, faecal samples obtained from wild animals (boars, N=39; deer, N=62; other, N=6), wild birds (N=98), food-producing animals (cattle, N=40; pigs, N=56) and pets (cats and dogs, N=199). Isolation was performed in selective and non-selective medium, followed by identification of virulence genes of E. coli by PCR, and Salmonella serotyping. The mcr 1-5 genes were screened by PCR in all E. coli and Salmonella isolates recovered. Whole-genome sequencing was used to investigate the presence of AMR genes, multilocus sequence type and phylogeny of all pathogenic isolates, as well as mcr-carrying isolates. Results: The overall occurrence rate of E.coli and Salmonella was 77.8% (389/500) and 2.8% (14/500), respectively. Pathogenic E.coli was detected in 46.7% (50/107) of wild animals, 2.0% (2/98) of wild birds, 11.4% (11/96) of food-producing animals and 19.1% (38/199) of pets, while Salmonella was identified in 2.8% of wild animals (3/107), 3.1% (3/98) of wild birds, and 7.3% (7/96) of food-producing animals. The mcr-1 gene was detected in 1.8% (7/389) of the E. coli positive samples, all of them in isolates recovered from pigs (13.7%; 7/51). All this mcr-1-positive showed additional resistance phenotypes, including MDR, and several virulence genes. No cases of mcr genes were identified in Salmonella isolates. Conclusions: Pathogenic E. coli was significantly more frequent than Salmonella spp in the studied animals. Besides the general low prevalence of the resistance to colistin mediated by mcr genes, the only cases were identified in E. coli food-producing isolates, namely in pigs. Our results highlight the importance of a continuous surveillance of antimicrobial resistance in an OneHealth approach. Funding: This work has received funding from the European Union’s Horizon 2020 research and innovation programme (Grant Agreement No 773830) OHEJP Discover.

Aim:This study aimed to estimate the prevalence, to characterize, and to evaluate antimicrobial susceptibility of pathogenic E. coli and Salmonella spp.in stray cats and dogs. Methods:Between November 2019 and October 2020, 199 faecal samples were obtained from 102 cats and 97 dogs rescued by a kennel in Lisboa. Isolation was performed in selective and non-selective medium, followed by identification of virulence genes of E. coli by PCR, and Salmonella serotyping. Antimicrobial susceptibility testing (AST) was performed according to EUCAST. mcr-1-5 genes were detected by PCR. All pathogenic isolates were further characterized by WGS. Results:The overall rate of E.coli and Salmonella was 73.9% (147/199) and 0.5% (1/199), respectively. Pathogenic E. coli were detected in 19.6% dogs and 18.6% cats. No cases of STEC were found. AST revealed that 13.0% of the isolates were resistant (16.2% dogs vs. 10.0% cats). Resistance to ampicillin, tetracycline, sulfamethoxazole, and/or trimethoprimwere the most frequent. MDR was observed in four ETEC isolated from dogs, which have the same ST155 and blaTEM-1B, sul1, sul2, dfrA1, and tet(A) resistance genes. All commensal E. coli were susceptible to antimicrobials. Salmonella (S.4,5:i:-) was identified in a cat. Belongs to ST3478 and presents ac(6')-Iaa, blaTEM-1B, and sul2 resistance genes. None isolate was ESBL or harboured mcr genes. Conclusions:Some of the isolates have relevant antimicrobial resistance. Due to the close contact with humans and to the potential release of pathogenic and resistant enteric bacteria in the environment, it is important to monitor stray cats and dogs health status following a One Health approach. Funding:This work is part of DiSCoVeR project (JRP24-R2-FBZ2-DiSCoVeR), funded by the European Union’s Horizon 2020 Research and Innovation programme under grant agreement No. 773830.

Although it is important to communicate scientific knowledge, it seems that this assumption is even more evident when it comes to environment-related themes, which have gained more relevance in the public sphere in the last decades. This article evaluates the spectacularization techniques of technology and science displayed as if they were the only solution to the environmental crisis. Firstly, from a literature perspective, this article shows the evolution of science communication and the relationship between society and science over the last centuries. After that, it presents a critical view on the excessive optimism around technology and scientific advancement, arguing that the possible solutions to the environmental crisis cannot come exclusively through technology substitution. The article thus shows that there is today a clear distinction between science communication and the communication of science as a mass product, which is promoted today by different stakeholders to manipulate public opinion for different reasons. Finally, the article identifies some common elements of this phenomenon that we call technowashing, insofar as it aims at a laundering of responsibilities and harmful impacts of business and political decisions.

The management of abdominal aortic aneurysms, especially ruptured abdominal aortic aneurysms, continues to challenge vascular surgeons. A ruptured abdominal aortic aneurysm is associated with a high mortality rate. If cardiopulmonary resuscitation is required before surgical repair, mortality rates are said to be even higher. However, cardiac arrest in patients with ruptured abdominal aortic aneurysm does not accurately predict a nonsalvageable state or preclude functional survival. In these cases, agressive management may be the only hope for survival, and cardiac arrest should not as such contraindicate repair. The objective of this study is to present a successful case of repair of ruptured abdominal aortic aneurysm after cardiac arrest., Portuguese Journal of Cardiac Thoracic and Vascular Surgery, Vol. 28 No. 3 (2021): July - September

Reportamos um caso clínico de um jovem de 38 anos, vítima de paragem cardiorrespiratória em fibrilhação ventricular. O doente foi recuperado após 7 minutos de suporte básico de vida mais 26 minutos de suporte avançado de vida com 5 choques pelo monitor- desfibrilhador e LUCAS adaptado até à recuperação da circulação espontânea. Após estabilização o doente foi internado na Unidade de Cuidados Intensivos Polivalentes e posteriormente transferido para o serviço de Cardiologia, onde efetuou avaliação da anatomia coronária com coronárias sem lesões. Após descartadas as principais causas para o sucedido e passiveis de tratamento, o doente teve indicação para a colocação de um cardioversor - desfibrilhador- implantável. O caso clínico enaltece a rápida atuação e o elo de ligação da cadeia de sobrevivência, com suporte básico de vida e o contacto precoce com o centro de orientação de doentes urgentes, essenciais para a chegada em tempo útil da equipa de emergência médica e posterior ressuscitação cardiopulmonar avançada. info:eu-repo/semantics/publishedVersion

O livro de atas da 1ª Conferência Lusófona de Terapia Ocupacional é uma iniciativa do Núcleo de Terapia Ocupacional da RACS (Rede Académica de ciências da Saúde da Lusofonia). Nesta primeira edição, em formato virtual, no seguimento da pandemia que assolou o planeta, foram apresentados assincronamente 15 pósteres em formato vídeo e disponibilizados em https://www.youtube.com/playlist?list=PLKUR7eWoyi2QAmZdHuB8qapIIA3PGxPVf . Foram também apresentadas de forma síncrona 14 comunicações livres que resultaram em 13 artigos completos que foram publicados na RevSALUS - Revista Científica Internacional da Rede Académica das Ciências da Saúde da Lusofonia – RACS - https://revsalus.com/index.php/RevSALUS. Uma comunicação oral encontra-se em artigo completo neste livro. Neste livro de Atas encontram-se publicados os resumos dos artigos completos publicados na RevSalus assim com os artigos curtos que originaram os vídeo-pósteres. Com mais de 100 participantes internacionais, esta edição obteve um sucesso considerável atendendo à sua novidade e constrangimentos pandémicos. Introdução: A Paralisia Cerebral (PC) é uma lesão do Sistema Nervoso Central com forte impacto na funcionalidade. As alterações neuromusculoesqueléticas, sensoriopercetivas e cognitivas originam frequentemente necessidades complexas de comunicação, cuja intervenção multidisciplinar passa amiudadamente pela implementação de Sistemas Aumentativos e Alternativos de Comunicação (SAAC). O terapeuta ocupacional pode colaborar para esta intervenção, sobretudo quando são envolvidas Tecnologias de Apoio (T A). Objetivo: Descrever o processo da Terapia Ocupacional (TO) na implementação de SAAC, em crianças com PC, verificando a influência nas atividades, cuja participação está restringida por défices na comunicação. Metodologia: Scoping Review desenvolvida com base no guia orientador Preferred Reporting Items for Systematic Reviews and Meta-Analyses extension for Scoping Reviews (PRISMA-ScR) e de acordo com o protocolo do Joanna Briggs Institute (JBI), encetada com pesquisa na b-on. Foi realizada uma análise categorial temática dos artigos com recurso ao software webQDA. Resultados: Foram selecionados 10 artigos, dos 353 estudos inicialmente encontrados, de acordo com os critérios de inclusão, demonstrando a contribuição do processo da TO, a importância de uma equipa multidisciplinar, bem como, a identificação de lacunas, que sugerem investimento em investigações futuras. Conclusão: A TO tem um papel pertinente na implementação de SAAC na: avaliação, com identificação das capacidades e necessidades a diferentes níveis, incluindo o posicionamento, tipo de acesso mais adequado e contextos; intervenção, através do posicionamento, da adaptação e treino de utilização de SAAC, de formação, consultadoria e advocacy das partes interessadas. A implementação de SAAC é uma área que ainda carece de investimento em investigação e formação. Introduction: Cerebral Palsy (CP) is a Central Nervous System lesion with strong impact on functionality. The neuromusculoskeletal, sensory-perceptive and cognitive changes frequently originate complex communication needs, whose multidisciplinary intervention frequently involves the implementation of Augmentative and Alternative Communication Systems (AACS). The Occupational Therapist can collaborate in this intervention, especially when assistive technologies are involved. Objective: To describe the process of Occupational Therapy (OT) in the implementation of AACS in children with CP, checking the influence on the activities in which participation is restricted by communication deficits. Methodology: Scoping Review based on the Preferred Reporting Items for Systematic Reviews and Meta-Analyses extension for Scoping Reviews (PRISMA-ScR) and according to the Joanna Briggs Institute (JBI) protocol, conducted using the b-on library. A thematic categorical analysis of the articles was performed using the webQDA software. Results: Ten articles were selected according to the inclusion criteria of the 353 studies initially found, demonstrating the contribution of the OT process, the importance of a multidisciplinary team, as well as the identification of gaps, which suggest investment in future research. Conclusion: OT has a relevant role in the implementation of an AACS: in the assessment, with identification of capacities and needs at different levels, including positioning, the most appropriate type of access and contexts and, in the intervention through positioning, adaptation and training in the use of the AACS, as well as training, consulting and advocacy of stakeholders. The implementation of AACSis an area that stil l needs investment in research and training. info:eu-repo/semantics/publishedVersion

OHEJP project DISCOVER