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1,244 results on '"Reichart, Bruno"'

102. Organ transplants of the future: planning for innovations including xenotransplantation.

Author

Cozzi, Emanuele, Schneeberger, Stefan, Bellini, Maria Irene, Berglund, Erik, Böhmig, Georg, Fowler, Kevin, Hoogduijn, Martin, Jochmans, Ina, Marckmann, Georg, Marson, Lorna, Neuberger, James, Oberbauer, Rainer, Pierson, Richard N., Reichart, Bruno, Scobie, Linda, White, Colin, and Naesens, Maarten

Subjects
TRANSPLANTATION of organs, tissues, etc., XENOTRANSPLANTATION, CLINICAL medicine, XENOGRAFTS, GENETIC models
Abstract

Summary: The future clinical application of animal‐to‐human transplantation (xenotransplantation) is of importance to society as a whole. Favourable preclinical data relevant to cell, tissue and solid organ xenotransplants have been obtained from many animal models utilizing genetic engineering and protocols of pathogen‐free husbandry. Findings have reached a tipping point, and xenotransplantation of solid organs is approaching clinical evaluation, the process of which now requires close deliberation. Such discussions include considering when there is sufficient evidence from preclinical animal studies to start first‐in‐human xenotransplantation trials. The present article is based on evidence and opinions formulated by members of the European Society for Organ Transplantation who are involved in the Transplantation Learning Journey project. The article includes a brief overview of preclinical concepts and biology of solid organ xenotransplantation, discusses the selection of candidates for first‐in‐human studies and considers requirements for study design and conduct. In addition, the paper emphasizes the need for a regulatory framework for xenotransplantation of solid organs and the essential requirement for input from public and patient stakeholders. [ABSTRACT FROM AUTHOR]

Published
2021
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111. Orthotopic heart transplantations in the pig-to-baboon model: 308.9

Author

Brandl, Ulrike, Michel, Sebastian, Erhardt, Matthias, Brennerv, Paolo, Burdorf, Lars, Bittmann, Iris, Rössle, Matthias, Mordstein, Volker, Baschnegger, Heiko, Bauer, Andreas, Hammer, Claus, Thein, Eckart, Reichart, Bruno, and Schmoeckel, Michael

Published
2007

141. Use of a special bioreactor for the cultivation of a new flexible polyurethane scaffold for aortic valve tissue engineering

Author

Aleksieva Genoveva, Hollweck Trixi, Thierfelder Nikolaus, Haas Ulrike, Koenig Fabian, Fano Cornelia, Dauner Martin, Wintermantel Erich, Reichart Bruno, Schmitz Christoph, and Akra Bassil

Subjects
Tissue engineering, Heart valve, Polyurethane scaffold, Static cultivation, Dynamic cultivation, Medical technology, R855-855.5
Abstract

Abstract Background Tissue engineering represents a promising new method for treating heart valve diseases. The aim of this study was evaluate the importance of conditioning procedures of tissue engineered polyurethane heart valve prostheses by the comparison of static and dynamic cultivation methods. Methods Human vascular endothelial cells (ECs) and fibroblasts (FBs) were obtained from saphenous vein segments. Polyurethane scaffolds (n = 10) were primarily seeded with FBs and subsequently with ECs, followed by different cultivation methods of cell layers (A: static, B: dynamic). Group A was statically cultivated for 6 days. Group B was exposed to low flow conditions (t1= 3 days at 750 ml/min, t2= 2 days at 1100 ml/min) in a newly developed conditioning bioreactor. Samples were taken after static and dynamic cultivation and were analyzed by scanning electron microscopy (SEM), immunohistochemistry (IHC), and real time polymerase chain reaction (RT-PCR). Results SEM results showed a high density of adherent cells on the surface valves from both groups. However, better cell distribution and cell behavior was detected in Group B. IHC staining against CD31 and TE-7 revealed a positive reaction in both groups. Higher expression of extracellular matrix (ICAM, Collagen IV) was observed in Group B. RT- PCR demonstrated a higher expression of inflammatory Cytokines in Group B. Conclusion While conventional cultivation method can be used for the development of tissue engineered heart valves. Better results can be obtained by performing a conditioning step that may improve the tolerance of cells to shear stress. The novel pulsatile bioreactor offers an adequate tool for in vitro improvement of mechanical properties of tissue engineered cardiovascular prostheses.

Published
2012
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