2,142 results on '"Redolfi, A."'
Search Results
102. Mitochondrial Ca2+ Signaling and Bioenergetics in Alzheimer’s Disease
- Author
-
Nikita Arnst, Nelly Redolfi, Annamaria Lia, Martina Bedetta, Elisa Greotti, and Paola Pizzo
- Subjects
calcium ,Alzheimer’s disease ,mitochondria ,bioenergetics ,neuron ,microglia ,Biology (General) ,QH301-705.5 - Abstract
Alzheimer’s disease (AD) is a hereditary and sporadic neurodegenerative illness defined by the gradual and cumulative loss of neurons in specific brain areas. The processes that cause AD are still under investigation and there are no available therapies to halt it. Current progress puts at the forefront the “calcium (Ca2+) hypothesis” as a key AD pathogenic pathway, impacting neuronal, astrocyte and microglial function. In this review, we focused on mitochondrial Ca2+ alterations in AD, their causes and bioenergetic consequences in neuronal and glial cells, summarizing the possible mechanisms linking detrimental mitochondrial Ca2+ signals to neuronal death in different experimental AD models.
- Published
- 2022
- Full Text
- View/download PDF
103. Superantigens, a Paradox of the Immune Response
- Author
-
Sofia Noli Truant, Daniela María Redolfi, María Belén Sarratea, Emilio Luis Malchiodi, and Marisa Mariel Fernández
- Subjects
staphylococcal superantigen ,enterotoxin ,toxin pathogenicity ,immunomodulation ,molecular and cellular targets ,Medicine - Abstract
Staphylococcal enterotoxins are a wide family of bacterial exotoxins with the capacity to activate as much as 20% of the host T cells, which is why they were called superantigens. Superantigens (SAgs) can cause multiple diseases in humans and cattle, ranging from mild to life-threatening infections. Almost all S. aureus isolates encode at least one of these toxins, though there is no complete knowledge about how their production is triggered. One of the main problems with the available evidence for these toxins is that most studies have been conducted with a few superantigens; however, the resulting characteristics are attributed to the whole group. Although these toxins share homology and a two-domain structure organization, the similarity ratio varies from 20 to 89% among different SAgs, implying wide heterogeneity. Furthermore, every attempt to structurally classify these proteins has failed to answer differential biological functionalities. Taking these concerns into account, it might not be appropriate to extrapolate all the information that is currently available to every staphylococcal SAg. Here, we aimed to gather the available information about all staphylococcal SAgs, considering their functions and pathogenicity, their ability to interact with the immune system as well as their capacity to be used as immunotherapeutic agents, resembling the two faces of Dr. Jekyll and Mr. Hyde.
- Published
- 2022
- Full Text
- View/download PDF
104. ZnSnO3 or Zn2SnO4/SnO2 Hierarchical Material? Insight into the Formation of ZnSn(OH)6 Derived Oxides
- Author
-
Davide Redolfi-Bristol, Lorenzo Branzi, Michele Back, Pietro Riello, Adolfo Speghini, Nicola Pinna, and Alvise Benedetti
- Subjects
crystal structure ,materials ,phase transitions ,zinc stannate ,Inorganic chemistry ,QD146-197 - Abstract
Piezoelectric materials are a class of compounds that is gaining increasing interest in various applications such as energy harvesting. During the last decade, lead-free ZnSnO3 perovskite ceramic has gained attention among the scientific community thanks to its unique symmetry-dependent and spontaneous polarization properties such as piezoelectricity and ferroelectricity. Nevertheless, only a few studies successfully prepared pure ZnSnO3, while most seem to mislead the product for its hydroxide precursor (ZnSn(OH)6) or a mixture of Zn2SnO4 and SnO2. In our work, we investigated the conversion of ZnSn(OH)6 at different temperatures (500, 600, 700, 750 and 800 °C) by X-ray powder diffraction analysis, and in-situ using synchrotron radiation up to 950 °C under ambient atmosphere and in a vacuum, to reproduce conventional reaction conditions. SEM and TEM have been used to understand the evolution of the particle shape and surface structure before and after the thermal treatments. Our results show the instability of the ZnSn(OH)6 phase, which converts into an amorphous structure at low temperature. Above 750 °C, the material segregates into Zn2SnO4 and SnO2, supporting the hypothesis that the thermal treatment of the hydroxide phase under typical conditions results in the formation of an oxide mixture rather than the phase pure ZnSnO3.
- Published
- 2022
- Full Text
- View/download PDF
105. Mild cognitive impairment with suspected nonamyloid pathology (SNAP)
- Author
-
Caroli, Anna, Prestia, Annapaola, Galluzzi, Samantha, Ferrari, Clarissa, van der Flier, Wiesje M, Ossenkoppele, Rik, Van Berckel, Bart, Barkhof, Frederik, Teunissen, Charlotte, Wall, Anders E, Carter, Stephen F, Schöll, Michael, Choo, Il Han, Grimmer, Timo, Redolfi, Alberto, Nordberg, Agneta, Scheltens, Philip, Drzezga, Alexander, Frisoni, Giovanni B, Weiner, Michael, Aisen, Paul, Petersen, Ronald, Jack, Clifford R, Jagust, William, Trojanowki, JQ, Toga, Arthur W, Beckett, Laurel, Green, Robert C, Gamst, Anthony, Saykin, Andrew J, Morris, John, Potter, William Z, Montine, Tom, Thomas, Ronald G, Donohue, Michael, Walter, Sarah, Dale, Anders, Bernstein, Matthew, Felmlee, Joel, Fox, Nick, Thompson, Paul, Schuff, Norbert, Alexander, Gene, DeCarli, Charles, Bandy, Dan, Koeppe, Robert A, Foster, Norm, Reiman, Eric M, Chen, Kewei, Mathis, Chet, Cairns, Nigel J, Taylor-Reinwald, Lisa, Shaw, Les, Lee, Virginia M-Y, Korecka, Magdalena, Crawford, Karen, Neu, Scott, Harvey, Danielle, Kornak, John, Foroud, Tatiana M, Potkin, Steven, Shen, Li, Kachaturian, Zaven, Frank, Richard, Snyder, Peter J, Molchan, Susan, Kaye, Jeffrey, Dolen, Sara, Quinn, Joseph, Schneider, Lon, Pawluczyk, Sonia, Spann, Bryan M, Brewer, James, Vanderswag, Helen, Heidebrink, Judith L, Lord, Joanne L, Johnson, Kris, Doody, Rachelle S, Villanueva-Meyer, Javier, Chowdhury, Munir, Stern, Yaakov, Honig, Lawrence S, Bell, Karen L, Morris, John C, and Mintun, Mark A
- Subjects
Biomedical and Clinical Sciences ,Clinical Sciences ,Aging ,Alzheimer's Disease ,Neurosciences ,Brain Disorders ,Acquired Cognitive Impairment ,Neurodegenerative ,Alzheimer's Disease including Alzheimer's Disease Related Dementias (AD/ADRD) ,Dementia ,2.1 Biological and endogenous factors ,Aetiology ,Neurological ,Aged ,Aged ,80 and over ,Cognitive Dysfunction ,Databases ,Factual ,Disease Progression ,Female ,Follow-Up Studies ,Humans ,Male ,Middle Aged ,Neurodegenerative Diseases ,Plaque ,Amyloid ,Predictive Value of Tests ,Alzheimer's Disease Neuroimaging Initiative ,Cognitive Sciences ,Neurology & Neurosurgery ,Clinical sciences - Abstract
ObjectivesThe aim of this study was to investigate predictors of progressive cognitive deterioration in patients with suspected non-Alzheimer disease pathology (SNAP) and mild cognitive impairment (MCI).MethodsWe measured markers of amyloid pathology (CSF β-amyloid 42) and neurodegeneration (hippocampal volume on MRI and cortical metabolism on [(18)F]-fluorodeoxyglucose-PET) in 201 patients with MCI clinically followed for up to 6 years to detect progressive cognitive deterioration. We categorized patients with MCI as A+/A- and N+/N- based on presence/absence of amyloid pathology and neurodegeneration. SNAPs were A-N+ cases.ResultsThe proportion of progressors was 11% (8/41), 34% (14/41), 56% (19/34), and 71% (60/85) in A-N-, A+N-, SNAP, and A+N+, respectively; the proportion of APOE ε4 carriers was 29%, 70%, 31%, and 71%, respectively, with the SNAP group featuring a significantly different proportion than both A+N- and A+N+ groups (p ≤ 0.005). Hypometabolism in SNAP patients was comparable to A+N+ patients (p = 0.154), while hippocampal atrophy was more severe in SNAP patients (p = 0.002). Compared with A-N-, SNAP and A+N+ patients had significant risk of progressive cognitive deterioration (hazard ratio = 2.7 and 3.8, p = 0.016 and p < 0.001), while A+N- patients did not (hazard ratio = 1.13, p = 0.771). In A+N- and A+N+ groups, none of the biomarkers predicted time to progression. In the SNAP group, lower time to progression was correlated with greater hypometabolism (r = 0.42, p = 0.073).ConclusionsOur findings support the notion that patients with SNAP MCI feature a specific risk progression profile.
- Published
- 2015
106. Delphi definition of the EADC‐ADNI Harmonized Protocol for hippocampal segmentation on magnetic resonance
- Author
-
Boccardi, Marina, Bocchetta, Martina, Apostolova, Liana G, Barnes, Josephine, Bartzokis, George, Corbetta, Gabriele, DeCarli, Charles, deToledo‐Morrell, Leyla, Firbank, Michael, Ganzola, Rossana, Gerritsen, Lotte, Henneman, Wouter, Killiany, Ronald J, Malykhin, Nikolai, Pasqualetti, Patrizio, Pruessner, Jens C, Redolfi, Alberto, Robitaille, Nicolas, Soininen, Hilkka, Tolomeo, Daniele, Wang, Lei, Watson, Craig, Wolf, Henrike, Duvernoy, Henri, Duchesne, Simon, Jack, Clifford R, Frisoni, Giovanni B, and Segmentation, EADC‐ADNI Working Group on the Harmonized Protocol for Manual Hippocampal
- Subjects
Neurosciences ,Alzheimer Disease ,Atrophy ,Consensus ,Delphi Technique ,Hippocampus ,Humans ,Image Processing ,Computer-Assisted ,Imaging ,Three-Dimensional ,Internationality ,Magnetic Resonance Imaging ,Neuroimaging ,Volumetry ,Manual segmentation ,Harmonization ,Anatomical landmarks ,Delphi procedure ,Alzheimer's disease ,Medial temporal Jobe ,Hippocampal atrophy ,Magnetic.resonance ,Standard operational procedures ,Enrichment ,MCI ,Reliability ,EADC-ADNI Working Group on the Harmonized Protocol for Manual Hippocampal Segmentation ,Magnetic resonance ,Medial temporal lobe ,Clinical Sciences ,Geriatrics - Abstract
BackgroundThis study aimed to have international experts converge on a harmonized definition of whole hippocampus boundaries and segmentation procedures, to define standard operating procedures for magnetic resonance (MR)-based manual hippocampal segmentation.MethodsThe panel received a questionnaire regarding whole hippocampus boundaries and segmentation procedures. Quantitative information was supplied to allow evidence-based answers. A recursive and anonymous Delphi procedure was used to achieve convergence. Significance of agreement among panelists was assessed by exact probability on Fisher's and binomial tests.ResultsAgreement was significant on the inclusion of alveus/fimbria (P = .021), whole hippocampal tail (P = .013), medial border of the body according to visible morphology (P = .0006), and on this combined set of features (P = .001). This definition captures 100% of hippocampal tissue, 100% of Alzheimer's disease-related atrophy, and demonstrated good reliability on preliminary intrarater (0.98) and inter-rater (0.94) estimates.DiscussionConsensus was achieved among international experts with respect to hippocampal segmentation using MR resulting in a harmonized segmentation protocol.
- Published
- 2015
107. Influence of crystallization temperature and atmosphere on the phase composition, microstructure and electrical properties of Ni–Mn–O thin films
- Author
-
Sebastian Redolfi and Klaus Reichmann
- Subjects
NTC Thermistor ,Thin film ,Chemical Solution Deposition ,Clay industries. Ceramics. Glass ,TP785-869 - Abstract
Nickel manganite thin films were prepared by chemical solution deposition from nitrate and acetate precursors on polished alumina substrates. By variation of crystallization temperature between 650 °C and 900 °C and atmosphere during cooling of the films, the morphology, phase formation and electrical properties of the films were investigated. The electrically conductive spinel phase was formed as a main phase independent of processing conditions exhibiting NTCR characteristics. Cooling down in nitrogen atmosphere favored the decomposition of the spinel phase into nickel oxide and a spinel phase with higher manganese content, but it also reduced the resistance drift of the samples. From this finding it can be deduced that a reduced oxygen partial pressure influences defect equilibria and by that decreases cation vacancy concentration. This supports the assumption that cation redistribution by a cation vacancy diffusion mechanism is a relevant mechanism of resistance drift in such thin films.
- Published
- 2021
- Full Text
- View/download PDF
108. Norms for Automatic Estimation of Hippocampal Atrophy and a Step Forward for Applicability to the Italian Population
- Author
-
Silvia De Francesco, Samantha Galluzzi, Nicola Vanacore, Cristina Festari, Paolo Maria Rossini, Stefano F. Cappa, Giovanni B. Frisoni, and Alberto Redolfi
- Subjects
magnetic resonance imaging ,automatic segmentation tools ,normative distribution ,hippocampal volume ,aging ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Abstract
IntroductionHippocampal volume is one of the main biomarkers of Alzheimer’s Dementia (AD). Over the years, advanced tools that performed automatic segmentation of Magnetic Resonance Imaging (MRI) T13D scans have been developed, such as FreeSurfer (FS) and ACM-Adaboost (AA). Hippocampal volume is considered abnormal when it is below the 5th percentile of the normative population. The aim of this study was to set norms, established from the Alzheimer’s Disease Neuroimaging Initiative (ADNI) population, for hippocampal volume measured with FS v.6.0 and AA tools in the neuGRID platform (www.neugrid2.eu) and demonstrate their applicability for the Italian population.MethodsNorms were set from a large group of 545 healthy controls belonging to ADNI. For each pipeline, subjects with segmentation errors were discarded, resulting in 532 valid segmentations for FS and 421 for AA (age range 56–90 years). The comparability of ADNI and the Italian Brain Normative Archive (IBNA), representative of the Italian general population, was assessed testing clinical variables, neuropsychological scores and normalized hippocampal volumes. Finally, percentiles were validated using the Italian Alzheimer’s disease Repository Without Borders (ARWiBo) as external independent data set to evaluate FS and AA generalizability.ResultsHippocampal percentiles were checked with the chi-square goodness of fit test. P-values were not significant, showing that FS and AA algorithm distributions fitted the data well. Clinical, neuropsychological and volumetric features were similar in ADNI and IBNA (p > 0.01). Hippocampal volumes measured with both FS and AA were associated with age (p < 0.001). The 5th percentile thresholds, indicating left/right hippocampal atrophy were respectively: (i) below 3,223/3,456 mm3 at 56 years and 2,506/2,415 mm3 at 90 years for FS; (ii) below 4,583/4,873 mm3 at 56 years and 3,831/3,870 mm3 at 90 years for AA. The average volumes computed on 100 cognitively intact healthy controls (CN) selected from ARWiBo were close to the 50th percentiles, while those for 100 AD patients were close to the abnormal percentiles.DiscussionNorms generated from ADNI through the automatic FS and AA segmentation tools may be used as normative references for Italian patients with suspected AD.
- Published
- 2021
- Full Text
- View/download PDF
109. Inter-Cohort Validation of SuStaIn Model for Alzheimer’s Disease
- Author
-
Damiano Archetti, Alexandra L. Young, Neil P. Oxtoby, Daniel Ferreira, Gustav Mårtensson, Eric Westman, Daniel C. Alexander, Giovanni B. Frisoni, Alberto Redolfi, and for Alzheimer’s Disease Neuroimaging Initiative and EuroPOND Consortium
- Subjects
alzheiemer’s disease ,patient subtyping ,patient staging ,SuStain model ,inter-cohort validation ,Information technology ,T58.5-58.64 - Abstract
Alzheimer’s disease (AD) is a neurodegenerative disorder which spans several years from preclinical manifestations to dementia. In recent years, interest in the application of machine learning (ML) algorithms to personalized medicine has grown considerably, and a major challenge that such models face is the transferability from the research settings to clinical practice. The objective of this work was to demonstrate the transferability of the Subtype and Stage Inference (SuStaIn) model from well-characterized research data set, employed as training set, to independent less-structured and heterogeneous test sets representative of the clinical setting. The training set was composed of MRI data of 1043 subjects from the Alzheimer’s disease Neuroimaging Initiative (ADNI), and the test set was composed of data from 767 subjects from OASIS, Pharma-Cog, and ViTA clinical datasets. Both sets included subjects covering the entire spectrum of AD, and for both sets volumes of relevant brain regions were derived from T1-3D MRI scans processed with Freesurfer v5.3 cross-sectional stream. In order to assess the predictive value of the model, subpopulations of subjects with stable mild cognitive impairment (MCI) and MCIs that progressed to AD dementia (pMCI) were identified in both sets. SuStaIn identified three disease subtypes, of which the most prevalent corresponded to the typical atrophy pattern of AD. The other SuStaIn subtypes exhibited similarities with the previously defined hippocampal sparing and limbic predominant atrophy patterns of AD. Subject subtyping proved to be consistent in time for all cohorts and the staging provided by the model was correlated with cognitive performance. Classification of subjects on the basis of a combination of SuStaIn subtype and stage, mini mental state examination and amyloid-β1-42 cerebrospinal fluid concentration was proven to predict conversion from MCI to AD dementia on par with other novel statistical algorithms, with ROC curves that were not statistically different for the training and test sets and with area under curve respectively equal to 0.77 and 0.76. This study proves the transferability of a SuStaIn model for AD from research data to less-structured clinical cohorts, and indicates transferability to the clinical setting.
- Published
- 2021
- Full Text
- View/download PDF
110. Targeting volume overload and overnight rostral fluid shift: A new perspective to treat sleep apnea
- Author
-
Perger, Elisa, Jutant, Etienne-Marie, and Redolfi, Stefania
- Published
- 2018
- Full Text
- View/download PDF
111. Can measuring hippocampal atrophy with a fully automatic method be substantially less noisy than manual segmentation over both 1 and 3 years?
- Author
-
Cover, Keith S., van Schijndel, Ronald A., Bosco, Paolo, Damangir, Soheil, and Redolfi, Alberto
- Published
- 2018
- Full Text
- View/download PDF
112. Ammonia Toxicity and Associated Protein Oxidation: A Single-Cell Surface Enhanced Raman Spectroscopy Study.
- Author
-
Redolfi-Bristol, Davide, Mangiameli, Alessandro, Yamamoto, Kenta, Marin, Elia, Zhu, Wenliang, Mazda, Osam, Riello, Pietro, and Pezzotti, Giuseppe
- Published
- 2024
- Full Text
- View/download PDF
113. Analysis of autogenic bifurcation processes resulting in river avulsion.
- Author
-
Barile, Gabriele, Redolfi, Marco, and Tubino, Marco
- Subjects
- *
SEDIMENT transport , *SUSTAINABLE design , *STREAM restoration , *INFORMATION design , *MEANDERING rivers , *SEDIMENTS - Abstract
River bifurcations are constituent components of multi-thread fluvial systems, playing a crucial role in their morphodynamic evolution and the partitioning of water and sediment. Although many studies have been directed at exploring bifurcation dynamics, the conditions under which avulsions occur, resulting in the complete abandonment of one branch, are still not well understood. To address this knowledge gap, we develop a novel 1D numerical model based on existing nodal point relations for sediment partitioning, which allows for the simulation of the morphodynamic evolution of a free bifurcation. Model results show that when the discharge asymmetry is so high that the shoaling branch does not transport sediments (partial avulsion conditions) the dominant branch undergoes significant degradation, leading to a higher inlet step between the bifurcates and further amplifying the discharge asymmetry. The degree of asymmetry is found to increase with the length of the downstream channels to the point that when they are sufficiently long, the shoaling branch is completely abandoned (full avulsion conditions). To complement our numerical findings, we also formulate a new analytical model that is able to reproduce the essential characteristics of the partial avulsion equilibrium, which enables us to identify the key parameters that control the transition between different configurations. In summary, this research sheds light on the fundamental processes that drive avulsion through the abandonment of river bifurcations. The insights gained from this study provide a foundation for further investigations and may offer valuable information for the design of sustainable river restoration projects. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
114. FDG‐PET markers of heterogeneity and different risk of progression in amnestic MCI.
- Author
-
Caminiti, Silvia Paola, De Francesco, Silvia, Tondo, Giacomo, Galli, Alice, Redolfi, Alberto, Perani, Daniela, Cappa, Stefano F., Cotelli, Maria, Marra, Camillo, Rossini, Paolo Maria, Spadin, Patrizia, Tagliavini, Fabrizio, Vanacore, Nicola, and Vecchio, Fabrizio
- Abstract
INTRODUCTION: Amnestic mild cognitive impairment (aMCI) is emerging as a heterogeneous condition. METHODS: We looked at a cohort of N = 207 aMCI subjects, with baseline fluorodeoxyglucose positron emission tomography (FDG‐PET), T1 magnetic resonance imaging, cerebrospinal fluid (CSF), apolipoprotein E (APOE), and neuropsychological assessment. An algorithm based on FDG‐PET hypometabolism classified each subject into subtypes, then compared biomarker measures and clinical progression. RESULTS: Three subtypes emerged: hippocampal sparing–cortical hypometabolism, associated with younger age and the highest level of Alzheimer's disease (AD)‐CSF pathology; hippocampal/cortical hypometabolism, associated with a high percentage of APOE ε3/ε4 or ε4/ε4 carriers; medial–temporal hypometabolism, characterized by older age, the lowest AD‐CSF pathology, the most severe hippocampal atrophy, and a benign course. Within the whole cohort, the severity of temporo‐parietal hypometabolism, correlated with AD‐CSF pathology and marked the rate of progression of cognitive decline. DISCUSSION: FDG‐PET can distinguish clinically comparable aMCI at single‐subject level with different risk of progression to AD dementia or stability. The obtained results can be useful for the optimization of pharmacological trials and automated‐classification models. Highlights: Algorithm based on FDG‐PET hypometabolism demonstrates distinct subtypes across aMCI;Three different subtypes show heterogeneous biological profiles and risk of progression;The cortical hypometabolism is associated with AD pathology and cognitive decline;MTL hypometabolism is associated with the lowest conversion rate and CSF‐AD pathology. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
115. Complications and Long-Term Outcomes after Combined Tibial Plateau Leveling Osteotomy and Tibial Tuberosity Transposition for Treatment of Concurrent Cranial Cruciate Ligament Rupture and Grade III or IV Medial Patellar Luxation.
- Author
-
Redolfi, Giovanna and Grand, Jean-Guillaume
- Published
- 2024
- Full Text
- View/download PDF
116. Modelling steady grain sorting in river bifurcations
- Author
-
Ragno, Niccolò, primary, Redolfi, Marco, additional, Tambroni, Nicoletta, additional, and Tubino, Marco, additional
- Published
- 2023
- Full Text
- View/download PDF
117. A Novel 3D‐Printed/Porous Conduit with Tunable Properties to Enhance Nerve Regeneration Over the Limiting Gap Length (Adv. Mater. Technol. 17/2023)
- Author
-
Redolfi‐Riva, Eugenio, primary, Pérez‐Izquierdo, Míriam, additional, Zinno, Ciro, additional, Contreras, Estefania, additional, Rodríguez‐Meana, Bruno, additional, Iberite, Federica, additional, Ricotti, Leonardo, additional, Micera, Silvestro, additional, and Navarro, Xavier, additional
- Published
- 2023
- Full Text
- View/download PDF
118. Can be metal layered hydroxide an alternative for hydride production by cold H2/Ar plasma treatment?
- Author
-
Dragassi, Marie-Charlotte, primary, Haj-Khlifa, Sonia, additional, Menguy, Nicolas, additional, Redolfi, Michaël, additional, and Ammar, Souad, additional
- Published
- 2023
- Full Text
- View/download PDF
119. In Situ Raman Study of Neurodegenerated Human Neuroblastoma Cells Exposed to Outer-Membrane Vesicles Isolated from Porphyromonas gingivalis
- Author
-
Pezzotti, Giuseppe, primary, Adachi, Tetsuya, additional, Imamura, Hayata, additional, Bristol, Davide Redolfi, additional, Adachi, Keiji, additional, Yamamoto, Toshiro, additional, Kanamura, Narisato, additional, Marin, Elia, additional, Zhu, Wenliang, additional, Kawai, Toshihisa, additional, Mazda, Osam, additional, Kariu, Toru, additional, Waku, Tomonori, additional, Nichols, Frank C., additional, Riello, Pietro, additional, Rizzolio, Flavio, additional, Limongi, Tania, additional, and Okuma, Kazu, additional
- Published
- 2023
- Full Text
- View/download PDF
120. Analysis of autogenic bifurcation processes resulting in river avulsion
- Author
-
Barile, Gabriele, primary, Redolfi, Marco, additional, and Tubino, Marco, additional
- Published
- 2023
- Full Text
- View/download PDF
121. Defining the length parameter in river bifurcation models: a theoretical approach
- Author
-
Redolfi, Marco, primary
- Published
- 2023
- Full Text
- View/download PDF
122. Virtual brain simulations reveal network-specific parameters in neurodegenerative dementias
- Author
-
Monteverdi, Anita, primary, Palesi, Fulvia, additional, Schirner, Michael, additional, Argentino, Francesca, additional, Merante, Mariateresa, additional, Redolfi, Alberto, additional, Conca, Francesca, additional, Mazzocchi, Laura, additional, Cappa, Stefano F., additional, Cotta Ramusino, Matteo, additional, Costa, Alfredo, additional, Pichiecchio, Anna, additional, Farina, Lisa M., additional, Jirsa, Viktor, additional, Ritter, Petra, additional, Gandini Wheeler-Kingshott, Claudia A. M., additional, and D’Angelo, Egidio, additional
- Published
- 2023
- Full Text
- View/download PDF
123. Complications and Long-Term Outcomes after Combined Tibial Plateau Leveling Osteotomy and Tibial Tuberosity Transposition for Treatment of Concurrent Cranial Cruciate Ligament Rupture and Grade III or IV Medial Patellar Luxation
- Author
-
Redolfi, Giovanna, additional and Grand, Jean-Guillaume, additional
- Published
- 2023
- Full Text
- View/download PDF
124. Iron(III) Sulfate-Mediated Synthesis of 2,5-Furandicarboxylic Acid Dimethyl Ester from Galactaric Acid
- Author
-
Trapasso, Giacomo, primary, Chícharo, Beatriz, additional, Gherardi, Thomas, additional, Redolfi-Bristol, Davide, additional, and Aricò, Fabio, additional
- Published
- 2023
- Full Text
- View/download PDF
125. Polysaccharide Layer-by-Layer Coating for Polyimide-Based Neural Interfaces
- Author
-
Eugenio Redolfi Riva, Angela D’Alessio, and Silvestro Micera
- Subjects
nanostructured coating ,layer-by-layer ,neural interface ,long-term biocompatibility ,bioelectronic medicine ,electrode–tissue interface ,Mechanical engineering and machinery ,TJ1-1570 - Abstract
Implantable flexible neural interfaces (IfNIs) are capable of directly modulating signals of the central and peripheral nervous system by stimulating or recording the action potential. Despite outstanding results in acute experiments on animals and humans, their long-term biocompatibility is hampered by the effects of foreign body reactions that worsen electrical performance and cause tissue damage. We report on the fabrication of a polysaccharide nanostructured thin film as a coating of polyimide (PI)-based IfNIs. The layer-by-layer technique was used to coat the PI surface due to its versatility and ease of manufacturing. Two different LbL deposition techniques were tested and compared: dip coating and spin coating. Morphological and physiochemical characterization showed the presence of a very smooth and nanostructured thin film coating on the PI surface that remarkably enhanced surface hydrophilicity with respect to the bare PI surface for both the deposition techniques. However, spin coating offered more control over the fabrication properties, with the possibility to tune the coating’s physiochemical and morphological properties. Overall, the proposed coating strategies allowed the deposition of a biocompatible nanostructured film onto the PI surface and could represent a valid tool to enhance long-term IfNI biocompatibility by improving tissue/electrode integration.
- Published
- 2022
- Full Text
- View/download PDF
126. Sleep Disorders in Adults with Prader–Willi Syndrome: Review of the Literature and Clinical Recommendations Based on the Experience of the French Reference Centre
- Author
-
Pauline Dodet, Federica Sanapo, Smaranda Leu-Semenescu, Muriel Coupaye, Alice Bellicha, Isabelle Arnulf, Christine Poitou, and Stefania Redolfi
- Subjects
Prader–Willi syndrome ,sleep disorders ,sleep-disordered breathing ,narcolepsy ,hypersomnia ,excessive daytime sleepiness ,Medicine - Abstract
Prader–Willi syndrome (PWS) is a rare, genetic, multisymptomatic, neurodevelopmental disease commonly associated with sleep alterations, including sleep-disordered breathing and central disorders of hypersomnolence. Excessive daytime sleepiness represents the main manifestation that should be addressed by eliciting the detrimental effects on quality of life and neurocognitive function from the patients’ caregivers. Patients with PWS have impaired ventilatory control and altered pulmonary mechanics caused by hypotonia, respiratory muscle weakness, scoliosis and obesity. Consequently, respiratory abnormalities are frequent and, in most cases, severe, particularly during sleep. Adults with PWS frequently suffer from sleep apnoea syndrome, sleep hypoxemia and sleep hypoventilation. When excessive daytime sleepiness persists after adequate control of sleep-disordered breathing, a sleep study on ventilatory treatment, followed by an objective measurement of excessive daytime sleepiness, is recommended. These tests frequently identify central disorders of hypersomnolence, including narcolepsy, central hypersomnia or a borderline hypersomnolent phenotype. The use of wake-enhancing drugs (modafinil, pitolisant) is discussed in multidisciplinary expert centres for these kinds of cases to ensure the right balance between the benefits on quality of life and the risk of psychological and cardiovascular side effects.
- Published
- 2022
- Full Text
- View/download PDF
127. Effect of flow unsteadiness on the long-term evolution of alternate bars
- Author
-
Carlin, M., primary, Redolfi, M., additional, and Tubino, M., additional
- Published
- 2020
- Full Text
- View/download PDF
128. EVENTOS ADVERSOS A MEDICAMENTOS (EAM), INSUFICIÊNCIA RENAL E HIPERCALEMIA NA PRÁTICA CLÍNICA.
- Author
-
Pellissari, Rafaela Sonsim de Oliveira, primary, Oliota, Ana Flávia Redolfi, additional, Penteado, Suelem Tavares da Silva, additional, and Sanches, Andréia Cristina Conegero, additional
- Published
- 2020
- Full Text
- View/download PDF
129. Congenital trans-mesenteric hernia as a cause of small intestine strangulation in a dog
- Author
-
G. Redolfi and J.G. Grand
- Subjects
Small Animals - Published
- 2023
130. Complications and Long-Term Outcomes After Prosthetic Capsule Replacement in 15 Dogs with Traumatic Hip Luxation
- Author
-
Giovanna Redolfi and Jean-Guillaume Grand
- Subjects
Small Animals - Abstract
Complications and long-term clinical outcomes for 15 dogs surgically treated for traumatic craniodorsal hip luxation by prosthetic capsule replacement (PCR) with a prosthetic ligament were retrospectively reviewed. A PCR technique with capsulorrhaphy was performed in all dogs using acetabular screws with washers and a femoral tunnel as anchor points for the polyester prosthetic ligament. A non–weight-bearing sling was not placed. Minimum 1 yr follow-up period was required for study inclusion. Two major complications (13.3%) consisting of craniodorsal hip reluxation (n = 1) and capital physeal fracture (n = 1) were observed. Minor complications (superficial skin necrosis) occurred in one case (6.7 %). The patient with craniodorsal hip reluxation underwent femoral head and neck ostectomy and was excluded from long-term analysis. In the 11 cases that returned for long-term (median, 3.8 yr; range, 19–75 mo) evaluation at the authors’ institution, 10/11 of the dogs were clinically sound. Three dogs did not return for long-term evaluation. However, telephone interview with owners minimum1 yr after surgery indicated normal limb function and absence of complications in all three cases. These results suggest that PCR with polyester prosthetic ligament can be successful in maintaining hip reduction in dogs with craniodorsal hip luxation.
- Published
- 2023
131. Screening of obstructive sleep apnea syndrome by the deep breathing technique
- Author
-
Mwetty Onanga, Sarah Joanny, Isabelle Rivals, Elisa Perger, Isabelle Arnulf, Stefania Redolfi, and Caroline Sevoz-Couche
- Subjects
Pulmonary and Respiratory Medicine ,Neurology ,Neurology (clinical) - Abstract
Obstructive sleep apnea syndrome (OSAS) is associated with alterations in heart rate variability (HRV) in relation to chronic autonomic dysfunction. We tested the ability of the deep breathing technique -a simple way to evaluate HRV- to identify OSAS patients.Consecutive patients referred for suspected OSAS (without obesity, diabetes and heart diseases) were included. They underwent a measure of HRV at rest, and of heart rate (HR) oscillations during expiration vs. inspiration (DeltaHROf 31 included subjects (77% male), 14 had mild to moderate OSAS (apnea/hypopnea index, AHI median [IQR]: 18 [12]), and 17 had no OSAS. The conventional HRV analysis did not reveal any difference between the groups with vs. without OSAS. However, the DeltaHRThe deep breathing technique accurately identifies a reduction in cardiac changes in patients with mild to moderate OSAS. It could be used as a simple screening tool to select patients for polysomnography.
- Published
- 2023
132. Spontaneous Afferent Activity Carves Olfactory Circuits
- Author
-
Nelly Redolfi and Claudia Lodovichi
- Subjects
activity ,spontaneous ,development ,topography ,plasticity ,olfaction ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Abstract
Electrical activity has a key role in shaping neuronal circuits during development. In most sensory modalities, early in development, internally generated spontaneous activity sculpts the initial layout of neuronal wiring. With the maturation of the sense organs, the system relies more on sensory-evoked electrical activity. Stimuli-driven neuronal discharge is required for the transformation of immature circuits in the specific patterns of neuronal connectivity that subserve normal brain function. The olfactory system (OS) differs from this organizational plan. Despite the important role of odorant receptors (ORs) in shaping olfactory topography, odor-evoked activity does not have a prominent role in refining neuronal wiring. On the contrary, afferent spontaneous discharge is required to achieve and maintain the specific diagram of connectivity that defines the topography of the olfactory bulb (OB). Here, we provide an overview of the development of olfactory topography, with a focus on the role of afferent spontaneous discharge in the formation and maintenance of the specific synaptic contacts that result in the topographic organization of the OB.
- Published
- 2021
- Full Text
- View/download PDF
133. Hypoxemia during sleep and overnight rostral fluid shift in pulmonary arterial hypertension: a pilot study
- Author
-
Etienne-Marie Jutant, David Montani, Caroline Sattler, Sven Günther, Olivier Sitbon, Gilles Garcia, Isabelle Arnulf, Marc Humbert, Thomas Similowski, and Stefania Redolfi
- Subjects
Diseases of the circulatory (Cardiovascular) system ,RC666-701 ,Diseases of the respiratory system ,RC705-779 - Abstract
Sleep-related breathing disorders, including sleep apnea and hypoxemia during sleep, are common in pulmonary arterial hypertension, but the underlying mechanisms remain unknown. Overnight fluid shift from the legs to the upper airway and to the lungs promotes obstructive and central sleep apnea, respectively, in fluid-retaining states. The main objective was to evaluate if overnight rostral fluid shift from the legs to the upper part of the body is associated with sleep-related breathing disorders in pulmonary arterial hypertension. In a prospective study, a group of stable patients with idiopathic, heritable, related to drugs, toxins, or treated congenital heart disease pulmonary arterial hypertension underwent a polysomnography and overnight fluid shift measurement by bioelectrical impedance in the month preceding or following a one-day hospitalization according to regular pulmonary arterial hypertension follow-up schedule with a right heart catheterization. Results show that among 15 patients with pulmonary arterial hypertension (women: 87%; median (25–75th percentiles); age: 40 (32–61) years; mean pulmonary arterial pressure 56 (46–68) mmHg; pulmonary vascular resistance 8.8 (6.4–10.1) Wood units), two patients had sleep apnea and eight (53%) had hypoxemia during sleep without apnea. The overnight rostral fluid shift was 168 (118–263) mL per leg. Patients with hypoxemia during sleep had a greater fluid shift (221 (141– 361) mL) than those without hypoxemia (118 (44–178) mL, p = 0.045). In conclusion, this pilot study suggests that hypoxemia during sleep is associated with overnight rostral fluid shift in pulmonary arterial hypertension.
- Published
- 2021
- Full Text
- View/download PDF
134. Experimental evidence for the interpretation of definite plural articles as markers of genericity – How Italian can help
- Author
-
Anna Czypionka, Michela Redolfi, Sergio Miguel Pereira Soares, and Tanja Kupisch
- Subjects
Definiteness ,Ambiguity ,Determiners ,Romance languages ,Germanic languages ,Language. Linguistic theory. Comparative grammar ,P101-410 - Abstract
In the Romance languages, definite plural articles (e.g., le rane ‘the frogs’) are generally ambiguous between a generic and a specific interpretation, and speakers must reconstruct the intended interpretation through the linguistic or extra-linguistic context. Following the “polar bear” paradigm implemented in Czypionka & Kupisch (2019)’s investigation on German, the goal of the present study is to check the suitability of their test on article semantics, by establishing to what extent native speakers of Italian interpret ambiguous definite plural DPs as generic or specific in the presence of a nonlinguistic picture context. We present judgment and reaction time data monitoring the preferred reading of sentences introduced by different kinds of noun phrases (e.g., Le rane/Queste rane/Le rane di solito sono verdi/gialle ‘The/These/Usually frogs are green/yellow’), while looking at pictures showing prototypical or non-prototypical properties (e.g., green vs. yellow frogs). Our results show that both possible interpretations of definite plural articles are routinely considered in Italian, despite the presence of a picture with specific referents, validating the “polar bear” paradigm as a suitable test of article semantics.
- Published
- 2021
- Full Text
- View/download PDF
135. Enhancement of CBRAM performance by controlled formation of a hourglass-shaped filament.
- Author
-
Attilio Belmonte, Ludovic Goux, Jiyong Woo, Umberto Celano, Augusto Redolfi, Sergiu Clima, and Gouri Sankar Kar
- Published
- 2017
- Full Text
- View/download PDF
136. E-Infrastructures for Neuroscientists: The GAAIN and neuGRID Examples
- Author
-
Orlandi, Daniele, Redolfi, Alberto, Revillard, Jérôme, Manset, David, Teipel, Stefan, Frisoni, Giovanni B., Patrizio, Giorgio, Editor-in-Chief, Canuto, Claudio, Series Editor, Coletti, Giulianella, Series Editor, Gentili, Graziano, Series Editor, Malchiodi, Andrea, Series Editor, Marcellini, Paolo, Series Editor, Mezzetti, Emilia, Series Editor, Moscariello, Gioconda, Series Editor, Ruggeri, Tommaso, Series Editor, Naldi, Giovanni, editor, and Nieus, Thierry, editor
- Published
- 2017
- Full Text
- View/download PDF
137. Faut-il s’occuper du sommeil de nos patients BPCO ?: Should we take care of the sleep of our COPD patients?
- Author
-
Dufay, P., Rédolfi, S., Trzepizur, W., and Grassion, L.
- Published
- 2022
- Full Text
- View/download PDF
138. Evaluation of a numerical model's ability to predict bed load transport observed in braided river experiments
- Author
-
Javernick, Luke, Redolfi, Marco, and Bertoldi, Walter
- Published
- 2018
- Full Text
- View/download PDF
139. Robotic Rehabilitation: An Opportunity to Improve Cognitive Functions in Subjects With Stroke. An Explorative Study
- Author
-
Irene Aprile, Giulia Guardati, Valeria Cipollini, Dionysia Papadopoulou, Alessia Mastrorosa, Letizia Castelli, Serena Monteleone, Alessandra Redolfi, Silvia Galeri, and Marco Germanotta
- Subjects
rehabilitation ,robotics ,stroke ,executive function ,attention ,memory ,Neurology. Diseases of the nervous system ,RC346-429 - Abstract
Background: After a stroke, up to three-quarters of acute and subacute stroke survivors exhibit cognitive impairment, with a significant impact on functional recovery, quality of life, and social engagement. Robotic therapy has shown its effectiveness on motor recovery, but its effectiveness on cognitive recovery has not fully investigated.Objective: This study aims to assess the impact of a technological rehabilitation intervention on cognitive functions in patients with stroke, using a set of three robots and one sensor-based device for upper limb rehabilitation.Methods: This is a pilot study in which 51 patients were enrolled. An upper limb rehabilitation program was performed using three robots and one sensor-based device. The intervention comprised motor/cognitive exercises, especially selected among the available ones to train also cognitive functions. Patients underwent 30 rehabilitation sessions, each session lasting 45 minutes, 5 days a week. Patients were assessed before and after the treatment with several cognitive tests (Oxford Cognitive Scale, Symbol Digit Modalities Test, Digit Span, Rey–Osterrieth Complex Figure, Tower of London, and Stroop test). In addition, motor (Fugl–Meyer Assessment and Motricity Index) and disability (modified Barthel Index) scales were used.Results: According to the Oxford Cognitive Scale domains, a significant percentage of patients exhibited cognitive deficits. Excluding perception (with only one patient impaired), the domain with the lowest percentage of patients showing a pathological score was praxis (about 25%), while the highest percentage of impaired patients was found in calculation (about 70%). After the treatment, patients improved in all the investigated cognitive domains, as measured by the selected cognitive assessment scales. Moreover, motor and disability scales confirmed the efficacy of robotics on upper limb rehabilitation in patients with stroke.Conclusions: This explorative study suggests that robotic technology can be used to combine motor and cognitive exercises in a unique treatment session.Clinical Trial Registration:www.ClinicalTrials.gov, identifier: NCT04164381.
- Published
- 2020
- Full Text
- View/download PDF
140. Neuroharmony: A new tool for harmonizing volumetric MRI data from unseen scanners
- Author
-
Rafael Garcia-Dias, Cristina Scarpazza, Lea Baecker, Sandra Vieira, Walter H.L. Pinaya, Aiden Corvin, Alberto Redolfi, Barnaby Nelson, Benedicto Crespo-Facorro, Colm McDonald, Diana Tordesillas-Gutiérrez, Dara Cannon, David Mothersill, Dennis Hernaus, Derek Morris, Esther Setien-Suero, Gary Donohoe, Giovanni Frisoni, Giulia Tronchin, João Sato, Machteld Marcelis, Matthew Kempton, Neeltje E.M. van Haren, Oliver Gruber, Patrick McGorry, Paul Amminger, Philip McGuire, Qiyong Gong, René S. Kahn, Rosa Ayesa-Arriola, Therese van Amelsvoort, Victor Ortiz-García de la Foz, Vince Calhoun, Wiepke Cahn, and Andrea Mechelli
- Subjects
Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Published
- 2020
- Full Text
- View/download PDF
141. Accelerated Aging Characterizes the Early Stage of Alzheimer’s Disease
- Author
-
Alessandro Leparulo, Marta Bisio, Nelly Redolfi, Tullio Pozzan, Stefano Vassanelli, and Cristina Fasolato
- Subjects
Alzheimer’s disease ,PS2APP ,presenilin-2 ,amyloid-β ,slow oscillations ,delta waves ,Cytology ,QH573-671 - Abstract
For Alzheimer’s disease (AD), aging is the main risk factor, but whether cognitive impairments due to aging resemble early AD deficits is not yet defined. When working with mouse models of AD, the situation is just as complicated, because only a few studies track the progression of the disease at different ages, and most ignore how the aging process affects control mice. In this work, we addressed this problem by comparing the aging process of PS2APP (AD) and wild-type (WT) mice at the level of spontaneous brain electrical activity under anesthesia. Using local field potential recordings, obtained with a linear probe that traverses the posterior parietal cortex and the entire hippocampus, we analyzed how multiple electrical parameters are modified by aging in AD and WT mice. With this approach, we highlighted AD specific features that appear in young AD mice prior to plaque deposition or that are delayed at 12 and 16 months of age. Furthermore, we identified aging characteristics present in WT mice but also occurring prematurely in young AD mice. In short, we found that reduction in the relative power of slow oscillations (SO) and Low/High power imbalance are linked to an AD phenotype at its onset. The loss of SO connectivity and cortico-hippocampal coupling between SO and higher frequencies as well as the increase in UP-state and burst durations are found in young AD and old WT mice. We show evidence that the aging process is accelerated by the mutant PS2 itself and discuss such changes in relation to amyloidosis and gliosis.
- Published
- 2022
- Full Text
- View/download PDF
142. DamC reveals principles of chromatin folding in vivo without crosslinking and ligation
- Author
-
Redolfi, Josef, Zhan, Yinxiu, Valdes-Quezada, Christian, Kryzhanovska, Mariya, Guerreiro, Isabel, Iesmantavicius, Vytautas, Pollex, Tim, Grand, Ralph S., Mulugeta, Eskeatnaf, Kind, Jop, Tiana, Guido, Smallwood, Sebastien A., de Laat, Wouter, and Giorgetti, Luca
- Published
- 2019
- Full Text
- View/download PDF
143. Postural lung volume reduction, expiratory flow limitation, and orthopnoea in diaphragmatic weakness: Preliminary observations
- Author
-
Redolfi, S., Straus, C., Ninane, V., and Similowski, T.
- Published
- 2024
- Full Text
- View/download PDF
144. Medical Informatics Platform (MIP): A Pilot Study Across Clinical Italian Cohorts
- Author
-
Alberto Redolfi, Silvia De Francesco, Fulvia Palesi, Samantha Galluzzi, Cristina Muscio, Gloria Castellazzi, Pietro Tiraboschi, Giovanni Savini, Anna Nigri, Gabriella Bottini, Maria Grazia Bruzzone, Matteo Cotta Ramusino, Stefania Ferraro, Claudia A. M. Gandini Wheeler-Kingshott, Fabrizio Tagliavini, Giovanni B. Frisoni, Philippe Ryvlin, Jean-François Demonet, Ferath Kherif, Stefano F. Cappa, and Egidio D'Angelo
- Subjects
Alzheimer's Dementia (AD) ,biomarkers ,diagnostic confidence ,Medical Informatics Platform (MIP) ,disease signature ,Neurology. Diseases of the nervous system ,RC346-429 - Abstract
Introduction: With the shift of research focus to personalized medicine in Alzheimer's Dementia (AD), there is an urgent need for tools that are capable of quantifying a patient's risk using diagnostic biomarkers. The Medical Informatics Platform (MIP) is a distributed e-infrastructure federating large amounts of data coupled with machine-learning (ML) algorithms and statistical models to define the biological signature of the disease. The present study assessed (i) the accuracy of two ML algorithms, i.e., supervised Gradient Boosting (GB) and semi-unsupervised 3C strategy (Categorize, Cluster, Classify—CCC) implemented in the MIP and (ii) their contribution over the standard diagnostic workup.Methods: We examined individuals coming from the MIP installed across 3 Italian memory clinics, including subjects with Normal Cognition (CN, n = 432), Mild Cognitive Impairment (MCI, n = 456), and AD (n = 451). The GB classifier was applied to best discriminate the three diagnostic classes in 1,339 subjects, and the CCC strategy was used to refine the classical disease categories. Four dementia experts provided their diagnostic confidence (DC) of MCI conversion on an independent cohort of 38 patients. DC was based on clinical, neuropsychological, CSF, and structural MRI information and again with addition of the outcome from the MIP tools.Results: The GB algorithm provided a classification accuracy of 85% in a nested 10-fold cross-validation for CN vs. MCI vs. AD discrimination. Accuracy increased to 95% in the holdout validation, with the omission of each Italian clinical cohort out in turn. CCC identified five homogeneous clusters of subjects and 36 biomarkers that represented the disease fingerprint. In the DC assessment, CCC defined six clusters in the MCI population used to train the algorithm and 29 biomarkers to improve patients staging. GB and CCC showed a significant impact, evaluated as +5.99% of increment on physicians' DC. The influence of MIP on DC was rated from “slight” to “significant” in 80% of the cases.Discussion: GB provided fair results in classification of CN, MCI, and AD. CCC identified homogeneous and promising classes of subjects via its semi-unsupervised approach. We measured the effect of the MIP on the physician's DC. Our results pave the way for the establishment of a new paradigm for ML discrimination of patients who will or will not convert to AD, a clinical priority for neurology.
- Published
- 2020
- Full Text
- View/download PDF
145. Heterologous Chimeric Construct Comprising a Modified Bacterial Superantigen and a Cruzipain Domain Confers Protection Against Trypanosoma cruzi Infection
- Author
-
María Belén Antonoglou, Andrés Sánchez Alberti, Daniela María Redolfi, Augusto Ernesto Bivona, María Julieta Fernández Lynch, Sofía Noli Truant, María Belén Sarratea, Laura Valeria Iannantuono López, Emilio Luis Malchiodi, and Marisa Mariel Fernández
- Subjects
cruzipain ,chimeric immunogen ,immune modulators ,Trypanosoma cruzi ,mutant superantigen ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Chagas disease is an endemic chronic parasitosis in Latin America affecting more than 7 million people. Around 100 million people are currently at risk of acquiring the infection; however, no effective vaccine has been developed yet. Trypanosoma cruzi is the etiological agent of this parasitosis and as an intracellular protozoan it can reside within different tissues, mainly muscle cells, evading host immunity and allowing progression towards the chronic stage of the disease. Considering this intracellular parasitism triggers strong cellular immunity that, besides being necessary to limit infection, is not sufficient to eradicate the parasite from tissues, a differential immune response is required and new strategies for vaccines against Chagas disease need to be explored. In this work, we designed, cloned and expressed a chimeric molecule, named NCz-SEGN24A, comprising a parasite antigen, the N-terminal domain of the major cysteine protease of T. cruzi, cruzipain (Nt-Cz), and a non-toxic form of the staphylococcal superantigen (SAg) G, SEG, with the residue Asn24 mutated to Ala (N24A). The mutant SAg SEGN24A, retains its ability to trigger classical activation of macrophages without inducing T cell apoptosis. To evaluate, as a proof of concept, the immunogenicity and efficacy of the chimeric immunogen vs. its individual antigens, C3H mice were immunized intramuscularly with NCz-SEGN24A co-adjuvanted with CpG-ODN, or the recombinant proteins Nt-Cz plus SEGN24A with the same adjuvant. Vaccinated mice significantly produced Nt-Cz-specific IgG titers after immunization and developed higher IgG2a than IgG1 titers. Specific cell-mediated immunity was assessed by in-vivo DTH and significant responses were obtained. To assess protection, mice were challenged with trypomastigotes of T. cruzi. Both schemes reduced the parasite load throughout the acute phase, but only mice immunized with NCz-SEGN24A showed significant differences against control; moreover, these mice maintained 100% survival. These results encourage testing mutated superantigens fused to specific antigens as immune modulators against pathogens.
- Published
- 2020
- Full Text
- View/download PDF
146. ¿Qué factores se asocian a las lesiones medulares traumáticas?
- Author
-
María Belén Redolfi, Jorge Luis Moviglia, and Ramiro Gonzalez Carey
- Subjects
rehabilitación neurológica ,traumatismos de la médula espinal ,complicaciones ,datos demógráficos ,cuadriplejía ,paraplejía ,Medicine - Abstract
Objetivo: Describir las características clínicas y demográficas de los sujetos con lesión medular espinal (LME) que se rehabilitaron en el Instituto Nacional de Rehabilitación Psicofísica del Sur (I.Na.Re.P.S.) e identificar si las mismas se encuentran asociadas a las lesiones traumáticas. Materiales y método: Estudio observacional, analítico, transversal, retrospectivo que incluyó sujetos con LME que ingresaron al I.Na.Re.P.S. entre enero de 2015 y agosto de 2018. Se realizó un análisis de regresión logística múltiple para identificar características asociadas al motivo de LME (traumática versus no traumática). Como medida de asociación se reportaron los Odds Ratios (ORs) con sus respectivos intervalos de confianza al 95% (IC 95%). Se consideró significativo un valor de p4 meses [OR 4,8 (IC 95% 1,3 - 17,2); p=0,015)] y lesión completa [OR 18,0 (IC 95% 3,5 - 93,9); p=0,015)] resultaron características independientes relacionadas con lesión medular traumática. Conclusiones: La LME afectó principalmente a hombres jóvenes, producto de lesiones traumáticas, causadas por accidentes de tránsito. La edad, el tiempo de tratamiento y la lesión de tipo completa fueron características independientes relacionadas con lesión medular traumática.
- Published
- 2020
147. MRI predictors of amyloid pathology: results from the EMIF-AD Multimodal Biomarker Discovery study
- Author
-
Mara ten Kate, Alberto Redolfi, Enrico Peira, Isabelle Bos, Stephanie J. Vos, Rik Vandenberghe, Silvy Gabel, Jolien Schaeverbeke, Philip Scheltens, Olivier Blin, Jill C. Richardson, Regis Bordet, Anders Wallin, Carl Eckerstrom, José Luis Molinuevo, Sebastiaan Engelborghs, Christine Van Broeckhoven, Pablo Martinez-Lage, Julius Popp, Magdalini Tsolaki, Frans R. J. Verhey, Alison L. Baird, Cristina Legido-Quigley, Lars Bertram, Valerija Dobricic, Henrik Zetterberg, Simon Lovestone, Johannes Streffer, Silvia Bianchetti, Gerald P. Novak, Jerome Revillard, Mark F. Gordon, Zhiyong Xie, Viktor Wottschel, Giovanni Frisoni, Pieter Jelle Visser, and Frederik Barkhof
- Subjects
Alzheimer’s disease ,Mild cognitive impairment ,Biomarkers ,Magnetic resonance imaging ,Amyloid ,Machine learning ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 ,Neurology. Diseases of the nervous system ,RC346-429 - Abstract
Abstract Background With the shift of research focus towards the pre-dementia stage of Alzheimer’s disease (AD), there is an urgent need for reliable, non-invasive biomarkers to predict amyloid pathology. The aim of this study was to assess whether easily obtainable measures from structural MRI, combined with demographic data, cognitive data and apolipoprotein E (APOE) ε4 genotype, can be used to predict amyloid pathology using machine-learning classification. Methods We examined 810 subjects with structural MRI data and amyloid markers from the European Medical Information Framework for Alzheimer’s Disease Multimodal Biomarker Discovery study, including subjects with normal cognition (CN, n = 337, age 66.5 ± 7.2, 50% female, 27% amyloid positive), mild cognitive impairment (MCI, n = 375, age 69.1 ± 7.5, 53% female, 63% amyloid positive) and AD dementia (n = 98, age 67.0 ± 7.7, 48% female, 97% amyloid positive). Structural MRI scans were visually assessed and Freesurfer was used to obtain subcortical volumes, cortical thickness and surface area measures. We first assessed univariate associations between MRI measures and amyloid pathology using mixed models. Next, we developed and tested an automated classifier using demographic, cognitive, MRI and APOE ε4 information to predict amyloid pathology. A support vector machine (SVM) with nested 10-fold cross-validation was applied to identify a set of markers best discriminating between amyloid positive and amyloid negative subjects. Results In univariate associations, amyloid pathology was associated with lower subcortical volumes and thinner cortex in AD-signature regions in CN and MCI. The multi-variable SVM classifier provided an area under the curve (AUC) of 0.81 ± 0.07 in MCI and an AUC of 0.74 ± 0.08 in CN. In CN, selected features for the classifier included APOE ε4, age, memory scores and several MRI measures such as hippocampus, amygdala and accumbens volumes and cortical thickness in temporal and parahippocampal regions. In MCI, the classifier including demographic and APOE ε4 information did not improve after additionally adding imaging measures. Conclusions Amyloid pathology is associated with changes in structural MRI measures in CN and MCI. An automated classifier based on clinical, imaging and APOE ε4 data can identify the presence of amyloid pathology with a moderate level of accuracy. These results could be used in clinical trials to pre-screen subjects for anti-amyloid therapies.
- Published
- 2018
- Full Text
- View/download PDF
148. Corrigendum: C1R Mutations Trigger Constitutive Complement 1 Activation in Periodontal Ehlers-Danlos Syndrome
- Author
-
Rebekka Gröbner, Ines Kapferer-Seebacher, Albert Amberger, Rita Redolfi, Fabien Dalonneau, Erik Björck, Di Milnes, Isabelle Bally, Veronique Rossi, Nicole Thielens, Heribert Stoiber, Christine Gaboriaud, and Johannes Zschocke
- Subjects
complement system ,connective tissue ,periodontitis ,C1r/s ,Ehlers-Danlos syndrome ,Immunologic diseases. Allergy ,RC581-607 - Published
- 2019
- Full Text
- View/download PDF
149. Axonal Odorant Receptors Mediate Axon Targeting
- Author
-
Ilaria Zamparo, Simona Francia, Sira Angela Franchi, Nelly Redolfi, Elisa Costanzi, Axelle Kerstens, Yosuke Fukutani, Roberto Battistutta, Patrizia Polverino de Laureto, Sebastian Munck, Bart De Strooper, Hiroaki Matsunami, and Claudia Lodovichi
- Subjects
Biology (General) ,QH301-705.5 - Abstract
Summary: In mammals, odorant receptors not only detect odors but also define the target in the olfactory bulb, where sensory neurons project to give rise to the sensory map. The odorant receptor is expressed at the cilia, where it binds odorants, and at the axon terminal. The mechanism of activation and function of the odorant receptor at the axon terminal is, however, still unknown. Here, we identify phosphatidylethanolamine-binding protein 1 as a putative ligand that activates the odorant receptor at the axon terminal and affects the turning behavior of sensory axons. Genetic ablation of phosphatidylethanolamine-binding protein 1 in mice results in a strongly disturbed olfactory sensory map. Our data suggest that the odorant receptor at the axon terminal of olfactory neurons acts as an axon guidance cue that responds to molecules originating in the olfactory bulb. The dual function of the odorant receptor links specificity of odor perception and axon targeting. : Odorant receptors play a critical role in the sensory map formation. Zamparo et al. find that axonal odorant receptors respond to cues expressed in the olfactory bulb. Among these, PEBP1 acts as a putative ligand of axonal receptors, and its genetic ablation results in a disrupted sensory map in vivo. Keywords: axon targeting, topographic map, olfactory bulb, axonal odorant receptors
- Published
- 2019
- Full Text
- View/download PDF
150. C1R Mutations Trigger Constitutive Complement 1 Activation in Periodontal Ehlers-Danlos Syndrome
- Author
-
Rebekka Gröbner, Ines Kapferer-Seebacher, Albert Amberger, Rita Redolfi, Fabien Dalonneau, Erik Björck, Di Milnes, Isabelle Bally, Veronique Rossi, Nicole Thielens, Heribert Stoiber, Christine Gaboriaud, and Johannes Zschocke
- Subjects
complement system ,connective tissue ,periodontitis ,C1r/s ,Ehlers-Danlos syndrome ,Immunologic diseases. Allergy ,RC581-607 - Abstract
Heterozygous missense or in-frame insertion/deletion mutations in complement 1 subunits C1r and C1s cause periodontal Ehlers-Danlos Syndrome (pEDS), a specific EDS subtype characterized by early severe periodontal destruction and connective tissue abnormalities like easy bruising, pretibial haemosiderotic plaques, and joint hypermobility. We report extensive functional studies of 16 C1R variants associated with pEDS by in-vitro overexpression studies in HEK293T cells followed by western blot, size exclusion chromatography and surface plasmon resonance analyses. Patient-derived skin fibroblasts were analyzed by western blot and Enzyme-linked Immunosorbent Assay (ELISA). Overexpression of C1R variants in HEK293T cells revealed that none of the pEDS variants was integrated into the C1 complex but cause extracellular presence of catalytic C1r/C1s activities. Variants showed domain-specific abnormalities of intracellular processing and secretion with preservation of serine protease function in the supernatant. In contrast to C1r wild type, and with the exception of a C1R missense variant disabling a C1q binding site, pEDS variants had different impact on the cell: retention of C1r fragments inside the cell, secretion of aggregates, or a new C1r cleavage site. Overexpression of C1R variants in HEK293T as well as western blot analyses of patient fibroblasts showed decreased levels of secreted C1r. Importantly, all available patient fibroblasts exhibited activated C1s and activation of externally added C4 in the supernatant while control cell lines secreted proenzyme C1s and showed no increase in C4 activation. The central elements in the pathogenesis of pEDS seem to be the intracellular activation of C1r and/or C1s, and extracellular presence of activated C1s that independently of microbial triggers can activate the classical complement cascade.
- Published
- 2019
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.