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776 results on '"Prakash, Louise"'

105. Requirement of the yeast RTH1 5' to 3' exonuclease for the stability of simple repetitive DNA

Author

Johnson, Robert E., Kovvali, Gopala, Prakash, Louise, and Prakash, Satya

Subjects
Exonucleases -- Research -- Genetic aspects, Colorectal cancer -- Research -- Genetic aspects, Yeast -- Genetic aspects -- Research, DNA mismatch repair -- Research -- Genetic aspects, Science and technology, Research, Genetic aspects
Abstract

Simple repetitive DNA sequences are unstable in human colorectal cancers and a variety of other cancers. Mutations in the DNA mismatch repair genes MSH2, MLH1, and PMS1 result in elevated [...]

Published
1995

110. A mechanism for the exclusion of low-fidelity human Y-family DNA polymerase from base excision repair

Author

Haracska, Lajos, Prakash, Louise, and Prakash, Satya

Subjects
DNA synthesis -- Research, DNA repair -- Research, DNA polymerases -- Research, Genetic research, Biological sciences
Abstract

A study was conducted to prove that Pol(sub lota), Pol(sub eta) and Pol(sub kappa) can each form a schiff base with 5' -deoxyribose (5'-dRP) residue containing DNA. However these enzyme-DNA complexes are not intermediates of a 5'-dRP lyase reaction, as 5'-dRP lyase activity was not found in purified Polh, Poli and Polk that were fully active in the DNA synthesis reaction.

Published
2003

111. Human DNA polymerase kappa uses template-primer misalignment as a novel means for extending mispaired termini and for generating single-base deletions

Author

Wolfle, William T., Washington, M. Todd, Prakash, Louise, and Prakash, Satya

Subjects
DNA synthesis -- Analysis, Nucleotides -- Genetic aspects, DNA polymerases -- Genetic aspects, Genetic research -- Analysis, Biological sciences
Abstract

Research has been conducted on human DNA polymerase. The authors demonstrate that this polymerase is adept at using template-primer misalignment for extention of mispaired termini and for generation of single-base delitions.

Published
2003

113. Accuracy of lesion bypass by yeast and human DNA Polymerase [Eta]

Author

Washington, M. Todd, Johnson, Robert E., Prakash, Louise, and Prakash, Satya

Subjects
DNA polymerases -- Research, Precancerous conditions -- Research, Yeast -- Research, Science and technology
Abstract

DNA polymerase [Eta](Pol[Eta]) functions in the error-free bypass of UV-induced DNA lesions, and a defect in Pol[Eta] in humans causes the cancer-prone syndrome, the variant form of xeroderma pigmentosum. Both yeast and human Pol[Eta] replicate through a cis-syn thymine-thymine dimer (TT dimer) by inserting two As opposite the two Ts of the dimer. Pol[Eta], however, is a low-fidelity enzyme, and it misinserts nucleotides with a frequency of [approximately equals] [10.sup.-2] to [10.sup.-3] opposite the two Ts of the TT dimer as well as opposite the undamaged template bases. This low fidelity of nucleotide insertion seems to conflict with the role of Pol[Eta] in the error-free bypass of UV lesions. To resolve this issue, we have examined the ability of human and yeast Pol[Eta] to extend from paired and mispaired primer termini opposite a TT dimer by using steady-state kinetic assays. We find that Pol[Eta] extends from mispaired primer termini on damaged and undamaged DNAs with a frequency of [approximately equals] [10.sup.-2] to [10.sup.-3] relative to paired primer termini. Thus, after the incorporation of an incorrect nucleotide, Pol[Eta] would dissociate from the DNA rather than extend from the mispair. The resulting primer-terminal mispair then could be subject to proofreading by a 3' [right arrow] 5' exonuclease. Replication through a TT dimer by Pol[Eta] then would be more accurate than that predicted from the fidelity of nucleotide incorporation alone.

Published
2001

114. Accuracy of thymine--thymine dimer bypass by Saccharomyces cerevisiae DNA polymerase [Eta]

Author

Washington, M. Todd, Johnson, Robert E., Prakash, Satya, and Prakash, Louise

Subjects
Saccharomyces -- Genetic aspects, DNA polymerases -- Research, DNA damage -- Research, Science and technology
Abstract

The Saccharomyces cerevisiae RAD30 gene functions in error-free replication of UV-damaged DNA. RAD30 encodes a DNA polymerase, Poi [Eta], which inserts two adenines opposite the two thymines of a cis-syn thymine-thymine (T-T) dimer. Here we use steady-state kinetics to determine the accuracy of DNA synthesis opposite the T-T dimer. Surprisingly, the accuracy of DNA synthesis opposite, the damaged DNA is nearly indistinguishable from that opposite non-damaged DNA, with frequencies of misincorporation of about [10.sup.-2] to [10.sup.-3]. These studies support the hypothesis that unlike most DNA polymerases, Pol [Eta] is able to tolerate distortions in DNA resulting from damage, which then enables the polymerase to utilize the intrinsic base pairing ability of the T-T dimer.

Published
2000

134. The architecture of yeast DNA polymerase zeta (927.2)

Author

Malik, Radhika, primary, Llorente, Yacob, additional, Jain, Rinku, additional, Choudhary, Jayati, additional, Johnson, Robert, additional, Prakash, Louise, additional, Prakash, Satya, additional, Ubarretxena‐Belandia, Iban, additional, and Aggarwal, Aneel, additional

Published
2014
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136. The Architecture of Yeast DNA Polymerase ζ

Author

Gómez-Llorente, Yacob, primary, Malik, Radhika, additional, Jain, Rinku, additional, Choudhury, Jayati Roy, additional, Johnson, Robert E., additional, Prakash, Louise, additional, Prakash, Satya, additional, Ubarretxena-Belandia, Iban, additional, and Aggarwal, Aneel K., additional

Published
2013
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