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2,770 results on '"Postma, Dirkje"'

109. Novel childhood asthma genes interact with in utero and early-life tobacco smoke exposure

Author

Scholtens, Salome, Postma, Dirkje S., Moffatt, Miriam F., Panasevich, Sviatlana, Granell, Raquel, Henderson, John A., Melén, Erik, Nyberg, Fredrik, Pershagen, Göran, Jarvis, Deborah, Ramasamy, Adaikalavan, Wjst, Matthias, Svanes, Cecilie, Bouzigon, Emmanuelle, Demenais, Florence, Kauffmann, Francine, Siroux, Valérie, von Mutius, Erika, Ege, Markus Johannes, Braun-Fahrländer, Charlotte, Genuneit, Jon, Brunekreef, Bert, Smit, Henriette A., Wijga, Alet H., Kerkhof, Marjan, Curjuric, Ivan, Imboden, Medea, Thun, Gian A., Probst-Hensch, Nicole, Freidin, Maxim B., Bragina, Elena Iu., Deev, I. A., Puzyrev, V. P., Daley, Denise, Park, Julie, Becker, Allan, Chan-Yeung, Moira, Kozyrskyj, Anita L., Pare, Peter, Marenholz, Ingo, Lau, Susanne, Keil, Thomas, Lee, Young-Ae, Kabesch, Michael, Wijmenga, Cisca, Franke, Lude, Nolte, Ilja M., Vonk, Judith, Kumar, Ashish, Farrall, Martin, Cookson, William O.C.M., Strachan, David P., Koppelman, Gerard H., and Boezen, Marike H.

Published
2014
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115. Asthma

Author

Holgate, Stephen T., Wenzel, Sally, Postma, Dirkje S., Weiss, Scott T., Renz, Harald, and Sly, Peter D.

Published
2015
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117. Poly(ethylene glycol)-based stable isotope labeling reagents for the quantitative analysis of low molecular weight metabolites by LC-MS

Author

Abello, Nicolas, Geurink, Paul P., van der Toorn, Marco, van Oosterhout, Antoon J.M., Lugtenburg, Johan, van der Marel, Gijs A., Kerstjens, Huib A.M., Postma, Dirkje S., Overkleeft, Hermen S., and Bischoff, Rainer

Subjects
Chemical tests and reagents -- Research, Metabolites -- Properties, Polyethylene glycol -- Properties, Isotopes -- Properties, Labels -- Methods, Liquid chromatography -- Methods, Mass spectrometry -- Methods, Molecular weights -- Research, Chemistry, Analytic -- Quantitative, Chemistry, Analytic -- Methods, Chemistry
Abstract

Stable isotope labeling (SIL) in combination with liquid chromatography--mass spectrometry is one of the most widely used quantitative analytical methods due to its sensitivity and ability to deal with extremely complex biological samples. However, SIL methods for metabolite analysis are still often limited in terms of multiplexing, the chromatographic properties of the derivatized analytes, or their ionization efficiency. Here we describe a new family of reagents for the SIL of primary amine-containing compounds based on pentafluorophenyl-activated esters of [sup.13]C-containing poly(ethylene glycol) chains (PEG) that addresses these shortcomings. A sequential buildup of the PEG chain allowed the introduction of various numbers of [sup.13]C atoms opening extended multiplexing possibilities. The PEG derivatives of rather hydrophilic molecules such as amino acids and glutathione were successfully retained on a standard [C.sub.18] reversed-phase column, and their identification was facilitated based on m/z values and retention times using extracted ion chromatograms. The mass increase due to PEG derivatization moved low molecular weight metabolite signals out of the often noisy, low m/z region of the mass spectra, which resulted in enhanced overall sensitivity and selectivity. Furthermore, elution at increased retention times resulted in efficient electrospray ionization due to the higher acetonitrile content in the mobile phase. The method was successfully applied to the quantification of intracellular amino acids and glutathione in a cellular model of human lung epithelium exposed to cigarette smoke-induced oxidative stress. It was shown that the concentration of most amino acids increased upon exposure of A549 cells to gas-phase cigarette smoke with respect to air control and cigarette smoke extract and that free thiol-containing species (e.g., glutathione) decreased although disulfide bond formation was not increased. These labeling reagents should also prove useful for the labeling of peptides and other compounds containing primary amine functionalities.

Published
2008

126. Exposure to TARC alters [[beta].sub.2]-adrenergic receptor signaling in human peripheral blood T lymphocytes

Author

Heijink, Irene H., Vellenga, Edo, Oostendorp, Jaap, de Monchy, Jan G.R., Postma, Dirkje S., and Kauffman, Henk F.

Subjects
T cells -- Research, Antigen receptors, T cell -- Research, Chemokines -- Research, Asthma -- Physiological aspects, T cells -- Receptors, Biological sciences
Abstract

The [[beta].sub.2]-adrenergic receptor ([[beta].sub.2]-AR) negatively regulates T cell activity through the activation of the [G.sub.8]/adenylyl cyclase/cAMP pathway. [[beta].sub.2]-AR desensitization, which can be induced by its phosphorylation, may have important consequences for the regulation of T cell function in asthma. In the present study we demonstrate that the C-C chemokine thymus and activation-regulated chemokine (TARC) impairs the ability of [[beta].sub.2]-agonist fenoterol to activate the cAMP downstream effector cAMP-responsive element binding protein (CREB) in freshly isolated human T cells. The TARC-induced activation of Src kinases resulted in membrane translocation of both G protein-coupled receptor kinase (GRK) 2 and [beta]-arrestin. Moreover, TARC was able to induce Src-dependent serine phosphorylation of the [[beta].sub.2]-AR as well as its association with GRK2 and [beta]-arrestin. Finally, in contrast to CREB, phosphorylation of Src and extracellular signal-regulated kinase was enhanced by fenoterol upon TARC pretreatment. In summary, we show for the first time that TARC exposure impairs [[beta].sub.2]-AR function in T cells. Our data suggest that this is mediated by Src-dependent activation of GRK2, resulting in receptor phosphorylation, binding to [beta]-arrestin, and a switch from cAMP-dependent signaling to activation of the MAPK pathway. We propose that aberrant T cell control in the presence of endogenous [beta]-agonists promotes T cell-mediated inflammation in asthma. cAMP-responsive element binding protein phosphorylation; Src kinases; G protein-coupled receptor kinase 2; [beta]-arrestin; thymus and activation-regulated chemokine

Published
2005

127. Fine mapping and positional candidate studies identify HLA-G as an asthma susceptibility gene on chromosome 6p21

Author

Nicolae, Dan, Cox, Nancy J., Lester, Lucille A., Schneider, Daniel, Tan, Zheng, Billstrand, Christine, Kuldanek, Susan, Donfack, Joseph, Kogut, Paul, Patel, Nina M., Goodenbour, Jeffrey, Howard, Timothy, Wolf, Raoul, Koppelman, Gerard H., White, Steven R., Parry, Rodney, Postma, Dirkje S., Meyers, Deborah, Bleecker, Eugene R., Hunt, Joan S., Solway, Julian, and Ober, Carole

Subjects
Human genome -- Research, Asthma -- Care and treatment, Asthma -- Research, Biological sciences
Published
2005

137. A Dynamic Bronchial Airway Gene Expression Signature of Chronic Obstructive Pulmonary Disease and Lung Function Impairment

Author

Steiling, Katrina, van den Berge, Maarten, Hijazi, Kahkeshan, Florido, Roberta, Campbell, Joshua, Liu, Gang, Xiao, Ji, Zhang, Xiaohui, Duclos, Grant, Drizik, Eduard, Si, Huiqing, Perdomo, Catalina, Dumont, Charles, Coxson, Harvey O., Alekseyev, Yuriy O., Sin, Don, Pare, Peter, Hogg, James C., McWilliams, Annette, Hiemstra, Pieter S., Sterk, Peter J., Timens, Wim, Chang, Jeffrey T., Sebastiani, Paola, OʼConnor, George T., Bild, Andrea H., Postma, Dirkje S., Lam, Stephen, Spira, Avrum, and Lenburg, Marc E.

Published
2013
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138. Of flies, mice and men: a systematic approach to understanding the early life origins of chronic lung disease

Author

Krauss-Etschmann, Susanne, Bush, Andrew, Bellusci, Saverio, Brusselle, Guy G, Dahlén, Sven Erik K, Dehmel, Stefan, Eickelberg, Oliver, Gibson, Greg, Hylkema, Machteld N, Knaus, Petra, Königshoff, Melanie, Lloyd, Clare M, Macciarini, Paolo, Mailleux, Arnaud, Marsland, Benjamin J, Postma, Dirkje S, Roberts, Graham, Samakovlis, Christos, Stocks, Janet, Vandesompele, Joke, Wjst, Matthias, and Holloway, John

Published
2013
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140. Authors’ response

Author

Fattahi, Fatemeh, Volbeda, Franke, Broekema, Martine, Lodewijk, Monique E, Hylkema, Machteld N, Reddel, Helen K, Timens, Wim, Postma, Dirkje S, and ten Hacken, Nick H T

Published
2013
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