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Made available in DSpace on 2020-12-12T01:13:32Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-03-30 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) BACKGROUND: Selenium (Se) is an essential element for humans and animals. Rice is one of the most commonly consumed cereals in the world, so the agronomic biofortification of cereals with Se may be a good strategy to increase the levels of daily intake of Se by the population. This study evaluated the agronomic biofortification of rice genotypes with Se and its effects on grain nutritional quality. Five rates of Se (0, 10, 25, 50, and 100 g ha−1) were applied as selenate via the soil to three rice genotypes under field conditions. RESULTS: Selenium concentrations in the leaves and polished grains increased linearly in response to Se application rates. A highly significant correlation was observed between the Se rates and the Se concentration in the leaves and grains, indicating high translocation of Se. The application of Se also increased the concentration of albumin, globulin, prolamin, and glutelin in polished grains. CONCLUSION: Biofortifying rice genotypes using 25 g Se ha−1 could increase the average daily Se intake from 4.64 to 66 μg day−1. Considering that the recommended daily intake of Se by adults is 55 μg day−1, this agronomic strategy could contribute to alleviating widespread Se malnutrition. © 2019 Society of Chemical Industry. São Paulo State University (UNESP) University of São Paulo (USP) School of Biosciences University of Nottingham The James Hutton Institute São Paulo State University (UNESP) FAPESP: 15/11690-3 CNPq: 309380/2017-0

The management of optimal soil pH is fundamental to sustainable crop production. Understanding the lime requirement for arable crops has developed gradually over the last several decades. The aim of this study was to examine the yield-pH relationship for a range of arable crops to understand their response to liming, based on the Long-Term Liming experiments established in 1962 at Rothamsted Research, UK. The main treatments of four different rates of lime and, therefore, four distinctly different soil pH levels were maintained for 35 years at two sites (Rothamsted and Woburn). The pH ranged from 4.4 to 8.0. The lime response was tested on the following crops: spring barley, spring oats, spring beans, spring lupins, winter lupins, potatoes, linseed, winter oilseed rape, winter triticale and winter wheat. Relative yield (RY) was used for non-linear regression analysis to detect site, year and phosphorus (P) fertiliser effects on the relationship with pH. Liming had a highly significant positive effect on soil pH, but overall there was no consistent increase or decrease in soil extractable P (Olsen) or exchangeable K. There were significant site effects detected for RY for most crops which reflect differences in the two soil types. Spring oats and potatoes had very weak responses to lime within the pH range tested. For spring barley, winter triticale, winter wheat and winter oilseed rape significant effects of P fertiliser on the yield-pH relationship were found, although the nature of effects differed between crops and sites. Findings from the Long-Term Liming experiment are invaluable in improving the fundamental understanding on the yield-pH relationship for important arable crops and this has significant implications on selecting crops for rotations. The pH at 90% RY was calculated for selected crops and the beneficial effect of fertiliser P was detected in significantly reducing the critical pH value.

Rhizoboxes are soil-root compartments that may well provide the closest naturalistic conditions for studying root systems architectures (RSAs) in controlled environments. Rhizobox-based studies can however lead to mis-estimation of root traits due to poor recovery of roots and loss of fine root features during washing of roots. We used a novel scanner-based rhizobox system to evaluate: (i) RSA traits of Brassica rapa genotypes; (ii) the relationship between root traits recorded from rhizoboxes and those of harvested roots and (iii) genotypic variation of seedlings in response to external P ([P] ext) availability. Brassica rapa genotypes were grown in soil-filled rhizoboxes abutting flatbed scanners and were watered once with either deionised water or a solution of 600 μM KH2PO4 to approximately 80% field capacity on a weight basis. Shoot and root P concentrations ([P]shoot and [P]root) of the B. rapa lines grown on different [P]ext were quantified. Visible root length at the surface of rhizoboxes constituted 85% of the total root length recovered from harvested root samples. High P supply induced a strong increase in [P]shoot in all genotypes (P < 0.001) whereas low P supply generally led to greater partitioning to roots. Seed P concentration and tissue P concentration were correlated only at low [P]ext. Total root length was strongly correlated with tissue P content under both low [P]ext (r = 0.81, P < 0.05) and high [P]ext (r = 0.82, P < 0.05) conditions. The novel scanner-based rhizobox system used addresses the substantial limitations associated with current use of rhizoboxes to study root growth dynamics.

Effects of soil depth and plant growth stages on arbuscular mycorrhizal fungal (AMF) colonization and community structure in maize roots and their potential contribution to host plant phosphorus (P) nutrition under different P-fertilizer inputs were studied. Research was conducted on a long-term field experiment over 3 years. AMF colonization was assessed by AM colonization rate and arbuscule abundances and their potential contribution to host P nutrition by intensity of fungal alkaline phosphatase (ALP)/acid phosphatase (ACP) activities and expressions of ZmPht1;6 and ZmCCD8a in roots from the topsoil and subsoil layer at different growth stages. AMF community structure was determined by specific amplification of 18S rDNA. Increasing P inputs up to 75-100 kg ha-1 yr-1 increased shoot biomass and P content but decreased AMF colonization and interactions between AMF and roots. AM colonization rate, intensity of fungal ACP/ALP activities, and expression of ZmPht1;6 in roots from the subsoil were greater than those from topsoil at elongation and silking but not at the dough stage when plants received adequate or excessive P inputs. Neither P input nor soil depth influenced the number of AMF operational taxonomic units (OTUs) present in roots, but P-fertilizer input, in particular, influenced community composition and relative AMF abundance. In conclusion, although increasing P inputs reduce AMF colonization and influence AMF community structure, AMF can potentially contribute to plant P nutrition even in well-fertilized soils, depending on the soil layer in which roots are located and the growth stage of host plants.

All plants must obtain a number of inorganic mineral elements from their environment to ensure successful growth and development of both vegetative and reproductive tissues. A total of fourteen mineral nutrients are required. Keywords: mineral requirements; essentiality; deficiency; metabolism; function

Crop genotypes that make best use of potassium (K) fertilizers can promote agricultural sustainability. However, screening germplasm collections for responses to K fertilizers is often laborious and expensive. To reduce costs, the number of K fertilizer treatments required to identify better genotypes should be minimized. This might be achieved by exploiting the mathematical relationships between biomass, plant K content, and K supply. This study employed 14 barley (Hordeum vulgare L.) genotypes growing in a hydroponics system that allowed the K supply to roots to be controlled through the K concentration in a flowing solution. It sought to determine the minimal number of treatments required to model the relationships between (a) shoot biomass and K supply, (b) plant K content and K supply, and (c) shoot biomass and plant K content. The relationships between (a) shoot biomass and K supply and (b) plant K content and K supply for any given genotype could be fitted by Michaelis–Menten equations and ...

The diets of many people lack sufficient zinc (Zn). This article investigates the production of potato (Solanum tuberosum L.) crops with greater tuber Zn concentrations to increase dietary Zn intakes. Field experiments were undertaken to increase Zn concentrations in potato tubers using foliar Zn fertilisers. Foliar Zn fertilisers increased tuber Zn concentrations but excessive applications reduced tuber yield in all four potato genotypes studied. Zinc oxide and zinc sulphate were more effective than zinc nitrate as foliar fertilisers for increasing tuber Zn concentrations whilst maintaining yields. Foliar Zn-fertilisers increased Zn concentrations in both flesh and skin of tubers. Although Zn-biofortification had no effect on the concentrations of iron, manganese, copper, calcium, magnesium or potassium in tubers, it increased tuber phytate concentrations. Cooking reduced Zn concentrations in Zn-biofortified tubers and this effect was exacerbated by peeling. After cooking, the quotient of Zn concentrations in Zn-biofortified/non-biofortified tubers ranged from 2.36 to 3.58. Applying foliar Zn fertilisers to a preceding potato crop increased Zn concentrations in grain of a following barley crop significantly in only one out of 3 years. Foliar Zn fertilisers can increase Zn concentrations in potato tubers and, potentially, dietary Zn intakes.

Rapeseed (Brassica napus L.) is extremely sensitive to both boron (B) and phosphorus (P) deficiencies. Application of chemical fertilizers is generally considered to be an effective agronomic practice to improve crop productivity, and it also affects soil bacterial community. However, there are few studies of the effects of balanced B and P fertilizer applications on crop yield and bacterial communities. In the present study, field experiments with five P application rates (0, 45, 90, 135 and 180 kg P2O5 ha−1) and four B application rates (0, 4.5, 9 and 18 kg Na2B4O7·5H2O ha−1) were conducted in 2016–2017 and 2017–2018 to investigate their effects on seed yield and P use efficiency (PUE) of B. napus. The smallest seed yields were obtained when B or P fertilizers were not applied (P90B0 or P0B9). Balanced B and P applications benefitted yields. The P45B4.5 treatment produced greater seed yield and PUE than the P45B18 treatment, and the P180B18 treatment produced greater seed yield and PUE than the P180B4.5 treatment. Sequencing of 16S rRNA genes revealed that the P90B9 treatment had greater soil bacterial diversity, and a different bacterial community composition, compared with the P90B0 or P0B9 treatments. Overall, our results underline the importance of balanced B and P nutrition for maximal seed yield of B. napus and the effects of B and P fertilizers on the soil bacterial community of B. napus.

Oilseed rape (Brassica napus L.) is an important crop in China. Although its yields are restricted by phosphorus (P) supply, the response of its root system to P supply has not been explored systematically. This study aimed to investigate the contribution of root morphological and biochemical traits to P acquisition from soils with deficient P (30 kg P2O5 ha−1) and sufficient P (90 kg P2O5 ha−1) supplies in the field at the leaf development, stem elongation, flowering, pod development and ripening stages. The total root length and root surface area in the surface soil (0−10 cm soil layer) were both reduced significantly by decreasing P supply. However, a larger root/shoot ratio and root length ratio were observed in plants with the deficient P supply at the flowering stage. Roots of plants with a deficient P supply also secreted more acid phosphatase and organic acid into the rhizosphere from stem elongation to pod development than plants with a sufficient P supply. Seed yield (SY), shoot dry weight (SDW) and total P content (TPC) were strongly correlated with root morphological traits at the leaf development and flowering stages, especially with the coarse root length (CRL) and root surface area in the surface soil. However, there were no correlations between SDW or TPC and root biochemical traits (rhizosphere pH, acid phosphatase activity and organic acid content). It is hypothesized that greater CRL in the surface soil (0−10 cm soil layer) at the leaf development and flowering stages, served as a scaffold for fine roots, enhancing soil exploration and P acquisition, and, thereby, increasing seed yield.

Shoot zinc (Zn) concentration in Brassica oleracea is affected by soil Zn and phosphorus (P) supply. Most problematic is the negative impact of P fertilizers on Zn concentrations in crops, which makes balancing yield and mineral quality challenging. To evaluate early molecular mechanisms involved in the accumulation of large shoot Zn concentrations regardless of the P supply, two B. oleracea accessions differing in root architecture and root exudates were grown hydroponically for two weeks with different combinations of P and Zn supply. Ionome profiling and deep RNA sequencing of roots revealed interactions of P and Zn in planta, without apparent phenotypic effects. In addition, increasing P supply did not reduce tissue Zn concentration. Substantial changes in gene expression in response to different P and/or Zn supplies in roots of both accessions ensured nutritionally sufficient P and Zn uptake. Numerous genes were differentially expressed after changing Zn or P supply and most of them were unique to only one accession, highlighting their different strategies in achieving nutrient sufficiency. Thus, different gene networks responded to the changing P and Zn supply in the two accessions. Additionally, enrichment analysis of gene ontology classes revealed that genes involved in lipid metabolism, response to starvation, and anion transport mechanisms were most responsive to differences in P and Zn supply in both accessions. The results agreed with previously studies demonstrating alterations in P and Zn transport and phospholipid metabolism in response to reduced P and Zn supply. It is anticipated that improved knowledge of genes responsive to P or Zn supply will help illuminate the roles in uptake and accumulation of P and Zn and might identify candidate genes for breeding high-yield-high-Zn brassicas.

Broccoli crops are produced throughout the year. They are grown under a wide range of environmental conditions, which results in large variation in the time to head initiation and in head growth after initiation. Mis-scheduling broccoli crops has financial consequences. Models to predict the harvest dates of broccoli crops allow growers to adapt their commercial strategies to maintain an appropriate supply of broccoli throughout the year in a cost-effective manner. The work described here developed models for the phenology and yields of Scottish broccoli crops using data from field trials and commercial crops that allow the prediction of harvest dates from observations or forecasts of daily temperatures and radiation. The models were validated using independent data obtained from commercial broccoli growers and used to explore the local, seasonal and year-to-year variation in the predicted harvest dates of broccoli in Scotland. The date of transplanting seedlings to the field had the largest effect on the time to harvest, although there was also considerable annual variation. Geographical location had least effect on the time to harvest.

Aims\ud Potato (Solanum tuberosum L.) has a large phosphorus (P)-fertiliser requirement. This is thought to be due to its inability to acquire P effectively from the soil. This work tested the hypothesis that early proliferation of its root system would enhance P acquisition, accelerate canopy development, and enable greater yields.\ud \ud Methods\ud Six years of field experiments characterised the relationships between (1) leaf P concentration ([P]leaf), tuber yield, and tuber P concentration ([P]tuber) among 27 Tuberosum, 35 Phureja and 4 Diploid Hybrid genotypes and (2) juvenile root vigour, P acquisition and tuber yield among eight Tuberosum genotypes selected for contrasting responses to P-fertiliser.\ud \ud Results\ud Substantial genetic variation was observed in tuber yield, [P]leaf and [P]tuber. There was a strong positive relationship between tuber yields and P acquisition among genotypes, whether grown with or without P-fertiliser. Juvenile root vigour was correlated with accelerated canopy development and both greater P acquisition and tuber biomass accumulation early in the season. However, the latter relationships became weaker during the season.\ud \ud Conclusions\ud Increased juvenile root vigour accelerated P acquisition and initial canopy cover and, thereby, increased tuber yields. Juvenile root vigour is a heritable trait and can be selected to improve P-fertiliser use efficiency of potato.

BACKGROUND: Linear relationships are commonly observed between shoot magnesium ([Mg](shoot)) and shoot calcium ([Ca](shoot)) concentrations among angiosperm species growing in the same environment. SCOPE AND CONCLUSIONS: This article argues that, in plants that do not exhibit ‘luxury’ accumulation of Mg or Ca, (1) distinct stoichiometric relationships between [Mg](shoot) and [Ca](shoot) are exhibited by at least three groups of angiosperm species, namely commelinid monocots, eudicots excluding Caryophyllales, and Caryophyllales species; (2) these relationships are determined by cell wall chemistry and the Mg/Ca mass quotients in their cell walls; (3) differences between species in [Mg](shoot) and [Ca](shoot) within each group are associated with differences in the cation exchange capacity (CEC) of the cell walls of different species; and (4) Caryophyllales constitutively accumulate more Mg in their vacuoles than other angiosperm species when grown without a supra-sufficient Mg supply.

Combined application of nitrogen (N) and potassium (K) fertilizer could significantly enhance crop yield. Crop yield and photosynthesis are inseparable. However, the influence of N and K interaction on photosynthesis is still not fully understood. Field and hydroponic experiments were conducted to examine the effects of N and K interaction on leaf photosynthesis characteristics and to explore the mechanisms in the hydroponic experiment. CO

Vegetation in the Arabian Peninsula is facing high and steadily rising tropospheric ozone pollution. However, little is known about the impacts of elevated ozone on date palms, one of the most important indigenous economic species. To elucidate the physiological responses of date palm to peak levels of acute ozone exposure, seedlings were fumigated with 200 ppb ozone for 8 h. Net CO2 assimilation rate, stomatal conduction, total carbon, its isotope signature and total sugar contents in leaves and roots were not significantly affected by the treatment and visible symptoms of foliar damage were not induced. Ozone exposure did not affect hydrogen peroxide and thiol contents but diminished the activities of glutathione reductase and dehydroascorbate reductase, stimulated the oxidation of ascorbate, and resulted in elevated total ascorbate contents. Total nitrogen, soluble protein and lignin contents remained unchanged upon ozone exposure, but the abundance of low molecular weight nitrogen (LMWN) compounds such as amino acids and nitrate as well as other anions were strongly diminished in leaves and roots. Other nitrogen pools did not benefit from the decline of LMWN, indicating reduced uptake and/or enhanced release of these compounds into the soil as a systemic response to aboveground ozone exposure. Several phenolic compounds, concurrent with fatty acids and stearyl alcohol, accumulated in leaves, but declined in roots, whereas total phenol contents significantly increased in the roots. Together these results indicate that local and systemic changes in both, primary and secondary metabolism contribute to the high tolerance of date palms to short-term acute ozone exposure.

Root traits such as root angle and hair length influence resource acquisition particularly for immobile nutrients like phosphorus (P). Here, we attempted to modify root angle in rice by disrupting the OsAUX1 auxin influx transporter gene in an effort to improve rice P acquisition efficiency. We show by X-ray microCT imaging that root angle is altered in the osaux1 mutant, causing preferential foraging in the top soil where P normally accumulates, yet surprisingly, P acquisition efficiency does not improve. Through closer investigation, we reveal that OsAUX1 also promotes root hair elongation in response to P limitation. Reporter studies reveal that auxin response increases in the root hair zone in low P environments. We demonstrate that OsAUX1 functions to mobilize auxin from the root apex to the differentiation zone where this signal promotes hair elongation when roots encounter low external P. We conclude that auxin and OsAUX1 play key roles in promoting root foraging for P in rice., Plant root architecture can adapt to different nutrient conditions in the soil. Here Giri et al. show that the rice auxin influx carrier AUX1 mobilizes auxin from the root apex to the differentiation zone and promotes root hair elongation when roots encounter low external phosphate.

Made available in DSpace on 2018-12-11T17:18:36Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-06-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Selenium (Se) is considered a beneficial element for plants; however, in high concentrations, it causes negative effects on plant physiology and development. This study reports the first physiological, nutritional, and ultrastructural description of Se toxicity in cowpea growing under field conditions. Selenium was supplied as a foliar application of sodium selenite at varying concentrations (0, 50, 100, 200, 400, 800, 1200, and 1600 g ha−1). An increased yield was observed with the application of 50 g ha−1 Se. Application of concentrations higher than 50 g ha−1 caused leaf toxicity. Increased lipid peroxidation and hydrogen peroxide concentration and reduced total sugars, sucrose, and carotenoid concentration were observed at highest doses tested (1200 and 1600 g ha−1). Applications of more than 50 g ha−1 Se reduced the phloem diameter, caused chlorosis of the leaf blade with a coalescence of lesions, and caused pink salt deposits to appear. Lesions were observed mainly near the trichomes on the adaxial surface of the leaf blade. An analysis of the element distribution with microprobe X-ray fluorescence spectrometry (μ-XRF) revealed accumulation of Se, calcium (Ca), potassium (K), copper (Cu), and manganese (Mn) near the primary vein and in the necrotic brown areas of the leaf lesions. In contrast, Na was homogeneously distributed in the leaf tissue. São Paulo State University – UNESP, Ilha Solteira, Postal Code 15.385-000 São Paulo State University – UNESP, Tupã Postal Code 17602-496, SP University of São Paulo – USP, Postal Code 13416-000, SP The James Hutton Institute, Invergowrie School of Biosciences The University of Nottingham, Sutton Bonington São Paulo State University – UNESP, Ilha Solteira, Postal Code 15.385-000 São Paulo State University – UNESP, Tupã Postal Code 17602-496, SP FAPESP: 2015/19121-8 FAPESP: 2016/19773-8

The selectivity of cation uptake by radish (Raphanus sativus L.) growing in the field near Chernobyl varies during the growth season. It is hypothesised that this is a consequence of variation in (137)Cs (Csss) and potassium (Kss) concentrations in the soil solution or the amount of dissolved potassium available to the plants. In the experiments reported here, it was observed that (1) Csss and Kss were positively correlated, (2) the selectivity for uptake of (137)Cs versus potassium (r) increased exponentially with decreasing Csss and Kss, and (3) the (137)Cs concentration, but not the potassium concentration, in plant material, increased abruptly upon the simultaneous reduction of Kss and Csss below about 10 μg ml(-1) and 6.7 Bq l(-1), respectively. It is thought that potassium enters root cells from the soil solution through constitutively-expressed, inward rectifying K(+) channels (KIRC) and K(+)/H(+)-symporters, whose abundance increases when plants become potassium-deficient. Cesium is thought to enter root cells through non-specific cation channels (NSCC) and, in plants lacking sufficient potassium, through K(+)/H(+)-symporters. It is argued that the increase in r, together with the abrupt increase (137)Cs concentration in plant tissues, when Kss and Csss decrease simultaneously cannot be attributed to competition between Cs(+) and K(+) for transport though KIRC, NSCC or K(+)/H(+)-symporters and that the most plausible explanation of these phenomena is an increase in the abundance of K(+)/H(+)-symporters in plants exhibiting incipient potassium deficiency.

Drought after the jointing stage restricted the growth and yield formation in wheat. Supplemental irrigation (SI) at Z31 (Zadoks Stage 31) reduced the ABA concentrations in the leaves of the inferior tillers (T3 and T10) and improved the survival rate of the tillers. Additional SI at Z60 reduced the ABA concentrations in the flag leaves of ear-bearing stems after anthesis and increased the assimilate accumulation and the 1000-grain weight. These two SIs increased the yield by 16.7–32.2 % compared with a rainfed control (W0). Delaying the first SI from Z31 to Z34 or Z39 reduced the ABA concentrations in the leaves of the main stem and superior tillers (T1 and T2) but increased the ABA concentrations in the leaves of the inferior tillers at Z45 and reduced the survival rate of the tillers. Delaying SI from Z31 and Z60 (W1) to Z34 and Z69 (W2) increased the partitioning of the recent (14C-labelled) assimilate to the stems plus sheaths and stalk plus glume at anthesis and the grains at maturity and increased the 1000-grain weight and the grain yield significantly. However, if SI was applied too late, i.e., at Z39 and Z77 (W3), the redistribution of the recent assimilate from the vegetative organs to the grain after anthesis and the grain yield at maturity were reduced. These data suggested that: (1) a lower soil moisture at the early jointing stage elevated the ABA concentrations in the inferior tillers and inhibited their growth; (2) if SI was applied too late (W3) senescence of the inferior tillers could not be halted; and (3) low ABA concentrations in the leaves during the later grain filling following the late SI (W3) were correlated with a reduced transfer of assimilates from the vegetative organs to the grain, leading to a significant reduction in the grain weight at maturity.

BACKGROUND AND AIMS: Root hairs increase the contact area of roots with soil and thereby enhance the capacity for solute uptake. The strict hair/non-hair pattern of Arabidopsis thaliana can change with nutrient deficiency or exposure to toxic elements, which modify root hair density. The effects of root hair density on cadmium (Cd) accumulation in shoots of arabidopsis genotypes with altered root hair development and patterning were studied. METHODS: Arabidopsis mutants that are unable to develop root hairs (rhd6-1 and cpc/try) or produce hairy roots (wer/myb23) were compared with the ecotype Columbia (Col-0). Plants were cultivated on nutrient agar for 2 weeks with or without Cd. Cadmium was applied as Cd(NO(3))(2) at two concentrations, 10 and 100 µm. Shoot biomass, root characteristics (primary root length, lateral root number, lateral root length and root hair density) and Cd concentrations in shoots were assessed. Anatomical features (suberization of the endodermis and development of the xylem) that might influence Cd uptake and translocation were also examined. KEY RESULTS: Cadmium inhibited plant growth and reduced root length and the number of lateral roots and root hairs per plant. Suberin lamellae in the root endodermis and xylem differentiation developed closer to the root apex in plants exposed to 100 µm Cd. The latter effect was genotype dependent. Shoot Cd accumulation was correlated with root hair abundance when plants were grown in the presence of 10 µm Cd, but not when grown in the presence of 100 µm Cd, in which treatment the development of suberin lamellae closer to the root tip appeared to restrict Cd accumulation in shoots. CONCLUSIONS: Root hair density can have a large effect on Cd accumulation in shoots, suggesting that the symplasmic pathway might play a significant role in the uptake and accumulation of this toxic element.

Root traits such as root angle and hair length influence resource acquisition particularly for immobile nutrients like phosphorus (P). Here, we attempted to modify root angle in rice by disrupting the OsAUX1 auxin influx transporter gene in an effort to improve rice P acquisition efficiency. We show by X-ray microCT imaging that root angle is altered in the osaux1 mutant, causing preferential foraging in the top soil where P normally accumulates, yet surprisingly, P acquisition efficiency does not improve. Through closer investigation, we reveal that OsAUX1 also promotes root hair elongation in response to P limitation. Reporter studies reveal that auxin response increases in the root hair zone in low P environments. We demonstrate that OsAUX1 functions to mobilize auxin from the root apex to the differentiation zone where this signal promotes hair elongation when roots encounter low external P. We conclude that auxin and OsAUX1 play key roles in promoting root foraging for P in rice.

The combined application of nitrogen (N) and zinc (Zn) fertilizers is a promising agronomic strategy for the biofortification of wheat grain with Zn for human nutrition. A glasshouse experiment was carried out to assess the effects of supplying N on the uptake, translocation and accumulation of Zn in tissues of two wheat genotypes (Quartzo and BRS Parrudo) with contrasting potential for grain Zn biofortification. Winter wheat genotypes were grown to maturity in 5 cm diameter, 100 cm length tubes filled with a mixture of sand, grit and gravel (40:40:20 v/v/v) over a layer of 0.1 m3 of gravel, and supplied a full nutrient solution with low Zn (0.15 μM) or high Zn (2.25 μM) and low N (0.4 mM) or high N (4.0 mM) concentrations. High N supply increased biomass production, Zn concentration and Zn content of straw and grain in both Quartzo and BRS Parrudo. Grain Zn content more than doubled when the supplies of Zn and N were both increased from low to high in both genotypes. Quartzo had a greater grain yield than BRS Parrudo. BRS Parrudo had greater grain Zn concentration and Zn content than Quartzo. A greater N supply promoted better uptake, translocation to the shoot and accumulation of Zn within the grain. Quartzo and BRS Parrudo differed in their partitioning of biomass and Zn between tissues. It might be possible to combine the greater grain yield of Quartzo with the greater grain Zn accumulation of BRS Parrudo to deliver a greatly improved genotype for human food security.

Background: There are numerous systems and techniques to measure the growth of plant roots. However, phenotyping large numbers of plant roots for breeding and genetic analyses remains challenging. One major difficulty is to achieve high throughput and resolution at a reasonable cost per plant sample. Here we describe a cost-effective root phenotyping pipeline, on which we perform time and accuracy benchmarking to identify bottlenecks in such pipelines and strategies for their acceleration.\ud \ud Results: Our root phenotyping pipeline was assembled with custom software and low cost material and equipment. Results show that sample preparation and handling of samples during screening are the most time consuming task in root phenotyping. Algorithms can be used to speed up the extraction of root traits from image data, but when applied to large numbers of images, there is a trade-off between time of processing the data and errors contained in the database.\ud \ud Conclusions: Scaling-up root phenotyping to large numbers of genotypes will require not only automation of sample preparation and sample handling, but also efficient algorithms for error detection for more reliable replacement of manual interventions.

Fertile soil is fundamental to our ability to achieve food security, but problems with soil degradation (such as acidification) are exacerbated by poor management. Consequently, there is a need to better understand management approaches that deliver multiple ecosystem services from agricultural land. There is global interest in sustainable soil management including the re-evaluation of existing management practices. Liming is a long established practice to ameliorate acidic soils and many liming-induced changes are well understood. For instance, short-term liming impacts are detected on soil biota and in soil biological processes (such as in N cycling where liming can increase N availability for plant uptake). The impacts of liming on soil carbon storage are variable and strongly relate to soil type, land use, climate and multiple management factors. Liming influences all elements in soils and as such there are numerous simultaneous changes to soil processes which in turn affect the plant nutrient uptake; two examples of positive impact for crops are increased P availability and decreased uptake of toxic heavy metals. Soil physical conditions are at least maintained or improved by liming, but the time taken to detect change varies significantly. Arable crops differ in their sensitivity to soil pH and for most crops there is a positive yield response. Liming also introduces implications for the development of different crop diseases and liming management is adjusted according to crop type within a given rotation. Repeated lime applications tend to improve grassland biomass production, although grassland response is variable and indirect as it relates to changes in nutrient availability. Other indicators of liming response in grassland are detected in mineral content and herbage quality which have implications for livestock-based production systems. Ecological studies have shown positive impacts of liming on biodiversity; such as increased earthworm abundance that provides habitat for wading birds in upland grasslands. Finally, understanding of liming impacts on soil and crop processes are explored together with functional aspects (in terms of ecosystems services) in a new qualitative framework that includes consideration of how liming impacts change with time. This holistic approach provides insights into the far-reaching impacts that liming has on ecosystems and the potential for liming to enhance the multiple benefits from agriculturally managed land. Recommendations are given for future research on the impact of liming and the implications for ecosystem services.

The prevalence of sodium (Na) “hyperaccumulator” species, which exhibit abnormally large shoot sodium concentrations ([Na]shoot) when grown in non-saline environments, was investigated among angiosperms in general and within the Caryophyllales order in particular. Shoot Na concentrations were determined in 334 angiosperm species, representing 35 orders, grown hydroponically in a non-saline solution. Many Caryophyllales species exhibited abnormally large [Na]shoot when grown hydroponically in a non-saline solution. The bimodal distribution of the log-normal [Na]shoot of species within the Caryophyllales suggested at least two distinct [Na]shoot phenotypes within this order. Mapping the trait of Na-hyperaccumulation onto the phylogenetic relationships between Caryophyllales families, and between subfamilies within the Amaranthaceae, suggested that the trait evolved several times within this order: in an ancestor of the Aizoaceae, but not the Phytolaccaceae or Nyctaginaceae, in ancestors of several lineages formerly classified as Chenopodiaceae, but not in the Amaranthaceae sensu stricto, and in ancestors of species within the Cactaceae, Portulacaceae, Plumbaginaceae,Tamaricaceae and Polygonaceae. In conclusion, a disproportionate number of Caryophyllales species behave as Na51 hyperaccumulators and multiple evolutionary origins of this trait can be identified within this order.

The flow of water through food commodity trade has been rationalized in the virtual water concept. Estimates of future virtual water flows under climate, land use, and population changes could have instrumental value for policy and strategic trade decisions. This paper estimated the virtual water flows associated with feed barley and meat imports to the UK under projected climate, land use, and population changes from the 2030s to the 2050s. The results show that future virtual water inflows associated with barley imports to balance domestic deficits are larger than total volume of water used in domestic barley production in the UK. Mean virtual water associated with total UK barley production ranged from 206 to 350 million m3. This is much less than the mean total virtual water associated with barley imports (if total barley produced in the UK is used for feed), which ranged from 2.5 to 5.6 billion m3 in the 2030s to the 2050s for all land use and climate change scenarios. If domestic barley production is distributed to the different end uses, the total virtual water inflows associated with imports to balance domestic feed barley supply could be as high as 7.4 billion m3. Larger virtual water inflows (as high as 9.9 billion m3) were associated with feed barley equivalent meat imports. While the UK barley production would be entirely green, imports of either barley or meat would result in large blue water inflows to the UK. Virtual water inflows increased across the time slices for all emissions scenarios, indicating weak effectiveness of yield or productivity gains to moderate virtual water inflows. While increase in yield and land allocated to barley production should be adaptive targets, the UK needs to take policy and strategic actions to diversify trade partners and shift imports away from countries where blue water flows can exacerbate existing or potential water stresses., Virtual water, Climate change, Land use change, Feed barley demand, Meat consumption, Population growth, Environmental science, Agriculture.

Background Phosphorus (P) deficiency limits crop production worldwide. Crops differ in their ability to acquire and utilise the P available. The aim of this study was to determine root traits (root exudates, root system architecture (RSA), tissue-specific allocation of P, and gene expression in roots) that (a) play a role in P-use efficiency and (b) contribute to large shoot zinc (Zn) concentration in Brassica oleracea. Results Two B. oleracea accessions (var. sabellica C6, a kale, and var. italica F103, a broccoli) were grown in a hydroponic system or in a high-throughput-root phenotyping (HTRP) system where they received Low P (0.025 mM) or High P (0.25 mM) supply for 2 weeks. In hydroponics, root and shoot P and Zn concentrations were measured, root exudates were profiled using both Fourier-Transform-Infrared spectroscopy and gas-chromatography-mass spectrometry and previously published RNAseq data from roots was re-examined. In HTRP experiments, RSA (main and lateral root number and lateral root length) was assessed and the tissue-specific distribution of P was determined using micro-particle-induced-X-ray emission. The C6 accession had greater root and shoot biomass than the F103 accession, but the latter had a larger shoot P concentration than the C6 accession, regardless of the P supply in the hydroponic system. The F103 accession had a larger shoot Zn concentration than the C6 accession in the High P treatment. Although the F103 accession had a larger number of lateral roots, which were also longer than in the C6 accession, the C6 accession released a larger quantity and number of polar compounds than the F103 accession. A larger number of P-responsive genes were found in the Low P treatment in roots of the F103 accession than in roots of the C6 accession. Expression of genes linked with “phosphate starvation” was up-regulated, while those linked with iron homeostasis were down-regulated in the Low P treatment. Conclusions The results illustrate large within-species variability in root acclimatory responses to P supply in the composition of root exudates, RSA and gene expression, but not in P distribution in root cross sections, enabling P sufficiency in the two B. oleracea accessions studied.

The morphology of roots and root systems influences the efficiency by which plants acquire nutrients and water, anchor themselves and provide stability to the surrounding soil. Plant genotype and the biotic and abiotic environment significantly influence root morphology, growth and ultimately crop yield. The challenge for researchers interested in phenotyping root systems is, therefore, not just to measure roots and link their phenotype to the plant genotype, but also to understand how the growth of roots is influenced by their environment. This review discusses progress in quantifying root system parameters (e.g. in terms of size, shape and dynamics) using imaging and image analysis technologies and also discusses their potential for providing a better understanding of root:soil interactions. Significant progress has been made in image acquisition techniques, however trade-offs exist between sample throughput, sample size, image resolution and information gained. All of these factors impact on downstream image analysis processes. While there have been significant advances in computation power, limitations still exist in statistical processes involved in image analysis. Utilizing and combining different imaging systems, integrating measurements and image analysis where possible, and amalgamating data will allow researchers to gain a better understanding of root:soil interactions.

A shortage of water threatens agricultural sustainability in the Huang-Huai-Hai Plain of China. Effective water-saving technologies need to be developed urgently. The experiment reported here, conducted between 2010 and 2012, aimed to determine how the length of the micro-sprinkling hose delivering supplemental irrigation affected photosynthetsis, dry matter (DM) accumulation, grain filling, and yield of winter wheat. Four treatments were compared: rainfed (W0) and irrigated with micro-sprinkling hoses with lengths of 40 m (W40), 60 m (W60), and 80 m (W80). The relative soil water content in the 0–140 cm soil horizon (RSWC) did not differ between 0 and 40 m from the proximal border of irrigated plots either in W40 or W60, and no differences in RSWC were observed across four inter-rows, spaced 22.9 cm apart, from the micro-sprinkling hoses in W40. However, RSWC decreased significantly with increasing distance from the proximal border in W80. There were no differences in mean actual photochemical efficiency (ΦPSII), maximum quantum yield of the PSII ( F v/ F m), flag leaf photosynthetic rate ( P n) or canopy apparent photosynthetic rate (CAP) 20 days after anthesis (DAA) between plants from W40 and W60, but all were greater in plants from W40 and W60 than in plants from W80. The ΦPSII, Fv/Fm, Pn, and CAP in plants from W80, but not W40, decreased significantly with increasing distance from the proximal border from 20 DAA. The total DM and the harvest index of plants from W40 were greater than those of plants from W60 and W80. The grain filling rate in the middle and later filling stages, 1000-grain weight, grain yield and water use efficiency became less as the length of micro-sprinkling hose was increased from 40 m to 80 m. In this study, the optimum length of micro-sprinkling hoses for irrigating wheat after jointing was 40 m to 60 m. The results indicate the importance of uniformity of irrigation water distribution in increasing the productivity of winter wheat.

Background Crops require adequate nutrition for the production of food, fibre and fuel, but soil conditions often limit the ability of crops to acquire mineral nutrients. To address this, mineral nutrients can be applied as inorganic or organic fertilisers to the soil or as liquid fertilisers to foliage. However, production and use of fertiliserscanhavenegativeenvironmentalimpacts.The articles in this Special Issue illustrate a number of ways to improve nutrient acquisition from the soil and their delivery through foliar application. Scope Articles highlighted here include those that discuss ways by which to assess a crop’s requirement for additional mineral elements, ways by which minerals can be supplied more effectively to crops both through roots and shoots, and ways by which the crop itself can be enhanced to acquire more mineral elements. Conclusions It is apparent from the information containedinthisSpecialIssue thattoimprovethe ability of crops to acquire mineral elements, a number of strategies are available. However, the success of any one intervention is dependent on how these strategies interact with the environment in which they are deployed and the suitability of the management system for the specific intervention.

The development of water-saving irrigation techniques is required for future food and ecological security in the Huang-Huai-Hai Plain of China, which suffers from severe water shortage. Field experiments were performed over two years (2010/2012) with the high-yielding winter wheat cultivar Jimai22 to examine the effects of supplemental irrigation (SI) with micro-sprinkling hoses on water distribution in soil and grain yield of winter wheat. Five irrigation treatments were tested: rainfed (T0), and irrigated with micro-sprinkling hoses with minimum sprinkling angles (i.e. the angle between the tangent of the initial water jet and the horizontal) of 35° (T1), 50° (T2), 65° (T3) and 80° (T4). The SI brought soil water content in the 0–140 cm profile to 75% field capacity (FC) at jointing and 70% FC at anthesis in 2010/2011, and 70% FC at both jointing and anthesis in 2011/2012. The distribution uniformity of irrigation water (Cμ) in soil after irrigation at jointing and anthesis was increased by increasing sprinkling angle from 35° to 80°. The increase in the soil water content in the 0–40 cm soil layer of the inter-rows decreased significantly with increasing distance from the micro-sprinkling hose in T1, T2 and T3, but there was no significant difference between inter-rows in T4. As sprinkling angle was increased from 35° to 80°, the amount of SI required (CIR), soil water consumption (ΔW) and crop evapotranspiration (ETc) decreased. The grain filling rate at the later filling stage, 1000-kernel weight, dry matter accumulation (DM), grain yield, and agronomic water use efficiency (AWUE) were significantly higher in T4 than in T1, T2 and T3. The CIR, ΔW and ETc had significant, negative, linear relationships with Cμ. However, DM, grain yield, and AWUE had significant, positive, linear relationships with Cμ with mean correlation coefficients of 0.60, 0.91 and 0.91, respectively. In this study, the optimum sprinkling angle of micro-sprinkling hoses for irrigating wheat after jointing was 80°.

Potatoes are a globally important source of food whose production requires large inputs of fertiliser and water. Recent research has highlighted the importance of the root system in acquiring resources. Here measurements, previously generated by field phenotyping, tested the effect of root size on maintenance of yield under drought (drought tolerance). Twelve potato genotypes, including genotypes with extremes of root size, were grown to maturity in the field under a rain shelter and either irrigated or subjected to drought. Soil moisture, canopy growth, carbon isotope discrimination and final yields were measured. Destructively harvested field phenotype data were used as explanatory variables in a general linear model (GLM) to investigate yield under conditions of drought or irrigation. Drought severely affected the small rooted genotype Pentland Dell but not the large rooted genotype Cara. More plantlets, longer and more numerous stolons and stolon roots were associated with drought tolerance. Previously measured carbon isotope discrimination did not correlate with the effect of drought. These data suggest that in-field phenotyping can be used to identify useful characteristics when known genotypes are subjected to an environmental stress. Stolon root traits were associated with drought tolerance in potato and could be used to select genotypes with resilience to drought.

Food security in the Huang-Huai-Hai Plain of China is threatened by water shortages and the early senescence of wheat induced by water deficit. However, effective water-saving irrigation techniques based on the consideration of precipitation, soil water storage and crop requirements are rudimentary. Information on the responses of transpiration, photosynthesis and plant senescence to Supplemental Irrigation (SI) at different stages of crop development is urgently required. Field experiments were performed in 2007–2008 and 2008–2009 to provide this information. Four irrigation treatments were tested: rainfed (W0), SI at Zadoks stage 31 (Z31) and Z60 (W1), SI at Z34 and Z69 (W2), and SI at Z39 and Z77 (W3). The SI brought soil water content in the 0–140 cm profile to 75% field capacity. Supplemental Irrigation increased grain yields and the scheduling of SI affected yield components. Delaying SI from Z31 and Z60 (W1) to Z34 and Z69 (W2) decreased the number of spikes, but increased the number of grains per spike, 1000-grain weight and crop yield. Activities of superoxide dismutase (SOD) and catalase (CAT) in flag leaves of plants from the W2 treatment were greater, and malondialdehyde (MDA) concentrations in flag leaves were lower, than those from the W3 treatment until 24 days after anthesis and those from the W1 and W0 treatments throughout anthesis. Although SI increased both photosynthetic rate (Pn) and transpiration rate ( E ), the net effect was greater instantaneous water use efficiency (WUE leaf = Pn/ E ). Supplemental Irrigation also increased agronomic Water Use Efficiency (grain yield/crop evapotranspiration). Delaying SI decreased the grain filling rate at the beginning of grain filling in 2007–2008, but increased the grain filling rate later in grain filling in both 2007–2008 and 2008–2009. An appropriate delay in SI (W2) increased grain yield substantially, but if SI was applied too late (W3), there was less effect on grain yield, probably because of an inhibition of assimilate remobilization to the grain due to delayed senescence.

Phosphorus (P) limits crop yield and P-fertilisers are frequently applied to agricultural soils. However, supplies of quality rock phosphate are diminishing. Plants have evolved mechanisms to improve P-acquisition and understanding these could improve the long-term sustainability of agriculture. Here we examined interactions between root hairs and arbuscular mycorrhizal (AM) colonisation in barley (Hordeum vulgare L.). Barley mutants exhibiting different root hair phenotypes, wild type barley and narrowleaf plantain (Plantago lanceolata L.) were grown in the glasshouse in P-sufficient and P-deficient treatments and allowed to develop AM colonization from the natural soil community. Plants were harvested after 6 weeks growth and root hair length, AM-fungal colonisation, shoot biomass and P-accumulation measured. Under P-deficient conditions, root hair length and AM colonisation were negatively related suggesting that resources are allocated to root hairs rather than to AM fungi in response to P-deficiency. There was evidence that barley and narrowleaf plantain employed different strategies to increase P-acquisition under identical conditions, but root hairs were more effective. This research suggests future barley breeding programmes should focus on maintaining or improving root hair phenotypes and that pursuing enhancements to AM associations under the prevalent agricultural conditions tested here would be ineffectual.

To avoid loss of yield, crops must maintain tissue potassium (K) concentrations above 5–40 mg K (g DM)–1. The supply of K from the soil is often insufficient to meet this demand and, in many agricultural systems, K fertilisers are applied to crops. However, K fertilisers are expensive. There is interest, therefore, in reducing applications of K fertilisers either by improving agronomy or developing crop genotypes that use K fertilisers more efficiently. Agronomic K fertiliser use efficiency is determined by the ability of roots to acquire K from the soil, which is referred to as K uptake efficiency (KUpE), and the ability of a plant to utilise the K acquired to produce yield, which is referred to as K utilisation efficiency (KUtE). There is considerable genetic variation between and within crop species in both KUpE and KUtE, and chromosomal loci affecting these characteristics have been identified in Arabidopsis thaliana and several crop species. Plant traits that increase KUpE include (1) exudation of organic compounds that release more non-exchangeable soil K, (2) high root K uptake capacity, (3) early root vigour, high root-to-shoot ratios, and high root length densities, (4) proliferation of roots throughout the soil volume, and (5) high transpiration rates. Plant traits that increase KUtE include (1) effective K redistribution within the plant, (2) tolerance of low tissue K concentrations, and, at low tissue K concentrations, (3) maintenance of optimal K concentrations in metabolically active cellular compartments, (4) replacement of K in its non-specific roles, (5) redistribution of K from senescent to younger tissues, (6) maintenance of water relations, photosynthesis and canopy cover, and (7) a high harvest index. The development of crop genotypes with these traits will enable K fertiliser applications to be reduced.

Genetics is broadly defined as the study of how genes control the characteristics of organisms. In this chapter, emphasis has been placed on differences in metabolic pathways, and their associated genes, that could account for variation in the ability of angiosperm species to tolerate large tissue selenium (Se) concentrations. The current view of the molecular biology of Se uptake and assimilation by plants is presented and differences between plant species likely to affect their ability to tolerate large tissue Se concentrations are identified. In particular, it is noted that plants that hyperaccumulate Se generally exhibit constitutive expression of genes encoding Se-transporters and enzymes involved in primary Se assimilation, biosynthesis of non-toxic Se metabolites and Se volatilisation. A plausible scheme for the evolution of differences in Se accumulation between angiosperm species is described. Since Se is an essential mineral element for animals, and the diets of many humans lack sufficient Se, the possibility of breeding crops with greater Se concentrations in their edible tissues is discussed. It is observed that, although Se concentrations in plants are largely determined by the phytoavailability of Se in the environment, there is significant intraspecific genetic variation in the Se concentrations of most edible crops that might be utilised to improve human diets. However, although molecular markers might be developed to known chromosomal quantitative trait loci (QTL) impacting Se concentration in edible tissues to assist breeding programmes, the actual genes underpinning this variation are largely unknown.

BACKGROUND Electrical capacitance, measured between an electrode inserted at the base of a plant and an electrode in the rooting substrate, is often linearly correlated with root mass. Electrical capacitance has often been used as an assay for root mass, and is conventionally interpreted using an electrical model in which roots behave as cylindrical capacitors wired in parallel. Recent experiments in hydroponics show that this interpretation is incorrect and a new model has been proposed. Here, the new model is tested in solid substrates. METHODS The capacitances of compost and soil were determined as a function of water content, and the capacitances of cereal plants growing in sand or potting compost in the glasshouse, or in the field, were measured under contrasting irrigation regimes. KEY RESULTS Capacitances of compost and soil increased with increasing water content. At water contents approaching field capacity, compost and soil had capacitances at least an order of magnitude greater than those of plant tissues. For plants growing in solid substrates, wetting the substrate locally around the stem base was both necessary and sufficient to record maximum capacitance, which was correlated with stem cross-sectional area: capacitance of excised stem tissue equalled that of the plant in wet soil. Capacitance measured between two electrodes could be modelled as an electrical circuit in which component capacitors (plant tissue or rooting substrate) are wired in series. CONCLUSIONS The results were consistent with the new physical interpretation of plant capacitance. Substrate capacitance and plant capacitance combine according to standard physical laws. For plants growing in wet substrate, the capacitance measured is largely determined by the tissue between the surface of the substrate and the electrode attached to the plant. Whilst the measured capacitance can, in some circumstances, be correlated with root mass, it is not a direct assay of root mass.

Background Mineral nutrient uptake and utilisation by plants are controlled by many traits relating to root morphology, ion transport, sequestration and translocation. The aims of this study were to determine the phenotypic diversity in root morphology and leaf and seed mineral composition of a polyploid crop species, Brassica napus L., and how these traits relate to crop habit. Traits were quantified in a diversity panel of up to 387 genotypes: 163 winter, 127 spring, and seven semiwinter oilseed rape (OSR) habits, 35 swede, 15 winter fodder, and 40 exotic/unspecified habits. Root traits of 14 d old seedlings were measured in a ‘pouch and wick’ system (n = ~24 replicates per genotype). The mineral composition of 3–6 rosette-stage leaves, and mature seeds, was determined on compost-grown plants from a designed experiment (n = 5) by inductively coupled plasma-mass spectrometry (ICP-MS). Results Seed size explained a large proportion of the variation in root length. Winter OSR and fodder habits had longer primary and lateral roots than spring OSR habits, with generally lower mineral concentrations. A comparison of the ratios of elements in leaf and seed parts revealed differences in translocation processes between crop habits, including those likely to be associated with crop-selection for OSR seeds with lower sulphur-containing glucosinolates. Combining root, leaf and seed traits in a discriminant analysis provided the most accurate characterisation of crop habit, illustrating the interdependence of plant tissues. Conclusions High-throughput morphological and composition phenotyping reveals complex interrelationships between mineral acquisition and accumulation linked to genetic control within and between crop types (habits) in B. napus. Despite its recent genetic ancestry (

This paper assessed the effect of projected climate change on the grain yield of barley in fourteen administrative regions in the United Kingdom (UK). Climate data for the 2030s, 2040s and 2050s for the high emission scenario (HES), medium emissions scenario (MES) and low emissions scenario (LES) were obtained from the UK Climate Projections 2009 (UKCP09) using the Weather Generator. Simulations were performed using the AquaCrop model and statistics of simulated future yields and baseline yields were compared. The results show that climate change could be beneficial to UK barley production. For all emissions scenarios and regions, differences between the simulated average future yields (2030s–2050s) and the observed yields in the baseline period (1961–1990) ranged from 1.4 to 4 tons·ha−1. The largest increase in yields and yield variability occurred under the HES in the 2050s. Absolute increases in yields over baseline yields were substantially greater in the western half of the UK than in the eastern regions but marginally from south to north. These increases notwithstanding, yield reductions were observed for some individual years due to saturated soil conditions (most common in Wales, Northern Ireland and South-West Scotland). These suggest risks of yield penalties in any growing season in the future, a situation that should be considered for planning adaptation and risk management.

A high-density SNP-based genetic linkage map was constructed and integrated with a previous map in the Tapidor x Ningyou7 (TNDH) Brassica napus population, giving a new map with a total of 2041 molecular markers and an average marker density which increased from 0.39 to 0.97 (0.82 SNP bin) per cM. Root and shoot traits were screened under low and ‘normal’ phosphate (Pi) supply using a ‘pouch and wick’ system, and had been screened previously in an agar based system. The P-efficient parent Ningyou7 had a shorter primary root length (PRL), greater lateral root density (LRD) and a greater shoot biomass than the P-inefficient parent Tapidor under both treatments and growth systems. Quantitative trait loci (QTL) analysis identified a total of 131 QTL, and QTL meta-analysis found four integrated QTL across the growth systems. Integration reduced the confidence interval by ~41%. QTL for root and shoot biomass were co-located on chromosome A3 and for lateral root emergence were co-located on chromosomes A4/C4 and C8/C9. There was a major QTL for LRD on chromosome C9 explaining ~18% of the phenotypic variation. QTL underlying an increased LRD may be a useful breeding target for P uptake efficiency in Brassica.

Background and Aims: Phosphate (Pi) deficiency in soils is a major limiting factor for crop growth worldwide. Plant growth under low Pi conditions correlates with root architectural traits and it may therefore be possible to select these traits for crop improvement. The aim of this study was to characterize root architectural traits, and to test quantitative trait loci (QTL) associated with these traits, under low Pi (LP) and high Pi (HP) availability in Brassica napus. Methods: Root architectural traits were characterized in seedlings of a double haploid (DH) mapping population (n = 190) of B. napus 'Tapidor' x 'Ningyou 7' (TNDH) using high-throughput phenotyping methods. Primary root length (PRL), lateral root length (LRL), lateral root number (LRN), lateral root density (LRD) and biomass traits were measured 12 d post-germination in agar at LP and HP. Key Results: In general, root and biomass traits were highly correlated under LP and HP conditions. 'Ningyou 7' had greater LRL, LRN and LRD than 'Tapidor', at both LP and HP availability, but smaller PRL. A cluster of highly significant QTL for LRN, LRD and biomass traits at LP availability were identified on chromosome A03; QTL for PRL were identified on chromosomes A07 and C06. Conclusions: High-throughput phenotyping of Brassica can be used to identify root architectural traits which correlate with shoot biomass. It is feasible that these traits could be used in crop improvement strategies. The identification of QTL linked to root traits under LP and HP conditions provides further insights on the genetic basis of plant tolerance to P deficiency, and these QTL warrant further dissection.

BACKGROUND Phosphorus (P) often limits crop production and is frequently applied as fertilizer; however, supplies of quality rock phosphate for fertilizer production are diminishing. Plants have evolved many mechanisms to increase their P acquisition, and an understanding of these traits could result in improved long-term sustainability of agriculture. This Viewpoint focuses on the potential benefits of root hairs to sustainable production. SCOPE First the various root-related traits that could be deployed to improve agricultural sustainability are catalogued, and their potential costs and benefits to the plant are discussed. A novel mathematical model describing the effects of length, density and longevity of root hairs on P acquisition is developed, and the relative benefits of these three root-hair traits to plant P nutrition are calculated. Insights from this model are combined with experimental data to assess the relative benefits of a range of root hair ideotypes for sustainability of agriculture. CONCLUSIONS A cost-benefit analysis of root traits suggests that root hairs have the greatest potential for P acquisition relative to their cost of production. The novel modelling of root hair development indicates that the greatest gains in P-uptake efficiency are likely to be made through increased length and longevity of root hairs rather than by increasing their density. Synthesizing this information with that from published experiments we formulate six potential ideotypes to improve crop P acquisition. These combine appropriate root hair phenotypes with architectural, anatomical and biochemical traits, such that more root-hair zones are produced in surface soils, where P resources are found, on roots which are metabolically cheap to construct and maintain, and that release more P-mobilizing exudates. These ideotypes could be used to inform breeding programmes to enhance agricultural sustainability.

Potatoes have an inadequate rooting system for efficient acquisition of water and minerals and use disproportionate amounts of irrigation and fertilizer. This research determines whether significant variation in rooting characteristics of potato exists, which characters correlate with final yield and whether a simple screen for rooting traits could be developed. Twenty-eight genotypes of Solanum tuberosum groups Tuberosum and Phureja were grown in the field; eight replicate blocks to final harvest, while entire root systems were excavated from four blocks. Root classes were categorised and measured. The same measurements were made on these genotypes in the glasshouse, 2 weeks post emergence. In the field, total root length varied from 40 m to 112 m per plant. Final yield was correlated negatively with basal root specific root length and weakly but positively with total root weight. Solanum tuberosum group Phureja genotypes had more numerous roots and proportionally more basal than stolon roots compared with Solanum tuberosum, group Tuberosum genotypes. There were significant correlations between glasshouse and field measurements. Our data demonstrate that variability in rooting traits amongst commercially available potato genotypes exists and a robust glasshouse screen has been developed. By measuring potato roots as described in this study, it is now possible to assess rooting traits of large populations of potato genotypes.

A retrospective analysis of the effects of nearly 75 years of breeding on the nitrogen use efficiency (NUE) of spring barley ( Hordeum vulgare L.) was undertaken to identify physiological mechanisms governing NUE and targets for future improvement. Fifteen varieties, selected to be as genetically dissimilar as possible and to represent the breeding period from 1931 to 2005, were grown at three site-year combinations in the NE of Scotland. Varieties were grown with zero or 110 kg N ha −1 supplied as ammonium nitrate. Averaged across site-years, breeding was shown to increase yield and NUE (grain yield N supply −1 ) by 1 and 1.2% per year respectively in the presence of fertilizer. Mean grain weight was larger in modern compared to old varieties grown both with and without N fertilizer. The greater response of yield to fertilizer in modern varieties was associated with the production of more grains m −2 . Significant variation was found between genotypes in efficiencies of N uptake (NupE; N offtake per unit N supply) and N utilization (NutE g , grain yield per unit N offtake). Differences in NutE g contributed 60% to the variation in NUE, whilst NupE contributed 40%. The improvement in NutE g was mostly the result of increases in harvest index (HI) and was stable across environments. NupE was positively correlated with post-anthesis, but not pre-anthesis, dry matter accumulation and N uptake, which suggests that greater NupE of some varieties was the result of an increased N demand from a large grain sink. Across all varieties NupE was not related to NutE g suggesting that improvements in each may be selected independently. The results also indicated possible opportunities for improving the efficiency of pre-anthesis N uptake.

Limitation of grain crop productivity by phosphorus (P) is widespread and will probably increase in the future. Enhanced P efficiency can be achieved by improved uptake of phosphate from soil (P-acquisition efficiency) and by improved productivity per unit P taken up (P-use efficiency). This review focuses on improved P-use efficiency, which can be achieved by plants that have overall lower P concentrations, and by optimal distribution and redistribution of P in the plant allowing maximum growth and biomass allocation to harvestable plant parts. Significant decreases in plant P pools may be possible, for example, through reductions of superfluous ribosomal RNA and replacement of phospholipids by sulfolipids and galactolipids. Improvements in P distribution within the plant may be possible by increased remobilization from tissues that no longer need it (e.g. senescing leaves) and reduced partitioning of P to developing grains. Such changes would prolong and enhance the productive use of P in photosynthesis and have nutritional and environmental benefits. Research considering physiological, metabolic, molecular biological, genetic and phylogenetic aspects of P-use efficiency is urgently needed to allow significant progress to be made in our understanding of this complex trait.

In the current issue, Guillaume et al. (2012) present a study reporting the soil-to-plant transfer of the caesium (Cs) and strontium (Sr) radionuclides, Cs and Sr, in an Alpine environment. These radionuclides were among those released and deposited in large quantities during above-ground weapons tests post-1945, and, more recently, as a consequence of the accidents including those at Chernobyl in 1986 (Smith et al. 2000), and Fukushima in 2011 (Yasunari et al. 2011). Due to their long half-lives (t1/2 Sr029.1 years; t1/2 Cs0 30.1 years) and high energy emissions of beta and gamma radiation, understanding and predicting the transfer of these radionuclides in the environment is of interest from a radioecological/health protection perspective. There is still considerable uncertainty regarding transfer and downstream effects of chronic exposure to low levels of ionising radiation on both humans and other biota (Beresford et al. 2004; Copplestone et al. 2010). One positive consequence of the release to the environment of long-lived anthropogenic radionuclides such as Cs and Sr is that they become de facto tracer elements which can be used to explore a range of environmental processes including nutrient cycling and soil erosion (e.g. Owens et al. 1996). Furthermore, studies of soil-to-plant transfer of Cs and Sr from the radioecological literature (e.g. Andersen 1967; Frissel et al. 2002) can be used to provide insights into the transport dynamics of the macronutrient elements K and Ca in plants (e.g. White and Broadley 2000, 2003), since these elements are chemical analogues of Cs and Sr, respectively. The study of Guillaume et al. (2012) is a valuable addition to this field. In Guillaume et al. (2012), soil and plant samples were taken at six sites from an Alpine valley, in Switzerland, at an elevation of 2,000 m. In total, 12 species of plant were sampled representing 12 plant families. Soil was sampled at three horizons. The authors conducted detailed soil-to-plant transfer analysis on these data. From their analyses, it is Plant Soil (2012) 355:23–27 DOI 10.1007/s11104-012-1163-1

Low phytoavailability of phosphorus (P) limits crop production worldwide. Increasing seed P content can improve plant establishment and increase yields. This is thought to be a consequence of faster initial root growth, which gives seedlings earlier access to growth-limiting resources, such as water and mineral elements. It can be calculated that seed P reserves can sustain maximal growth of cereal seedlings for several weeks after germination, until the plant has three or more leaves and an extensive root system. In this issue of Plant and Soil, Muhammad Nadeem and colleagues report (1) that measurable P uptake by roots of maize seedlings begins about 5 d after germination, (2) that the commencement of root P uptake is coincident with the transition from carbon heterotrophy to carbon autotrophy, and (3) that neither the timing nor the rate of uptake of exogenous P by the developing root system is influenced by initial seed P content. Here it is hypothesised that the delay in P acquisition by roots of maize seedlings might be explained if the expression of genes encoding phosphate transporters is not upregulated either (1) because the plant has sufficient P for growth or (2) because a systemic signal from the shoot, which relies on photosynthesis or phloem development, is not produced, translocated or perceived.