101. A preservation method that allows recovery of intact RNA from tissues dissected by laser capture microdissection
- Author
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Vincenzo Cuccurullo, Paolo Emidio Macchia, Annamaria Rosica, Luigi Mansi, Mario De Felice, J. Frederic Mushinski, James D. Owens, Rosanna Parlato, Roberto Di Lauro, Robert F. Bonner, Parlato, R, Rosica, A, Cuccurullo, Vincenzo, Mansi, Luigi, Macchia, P, Owens, Jd, Mushinski, Jf, DE FELICE, M, Bonner, Rf, DI LAURO, R., Parlato, Rosanna, Rosica, Annamaria, Macchia, PAOLO EMIDIO, Owens, J. D., Mushinski, J. F., DE FELICE, Mario, Bonner, R. F., and DI LAURO, Roberto
- Subjects
EXTRACTION ,Sucrose ,Biophysics ,Biology ,Polymerase Chain Reaction ,Biochemistry ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Histological diagnosis ,Gene expression ,Animals ,Humans ,FIXATION ,RNA, Messenger ,Thyroid Neoplasms ,Molecular Biology ,030304 developmental biology ,Laser capture microdissection ,Cryopreservation ,RNA metabolism ,Cell specific ,0303 health sciences ,cDNA library ,Dissection ,Lasers ,RNA ,AMPLIFICATION ,Cell Biology ,Blotting, Northern ,Embryo, Mammalian ,GENE ,Molecular biology ,Blot ,PCR ,030220 oncology & carcinogenesis ,Tissue Preservation - Abstract
We report a novel method for preparing samples for laser capture microdissection. The procedure described here permits extraction of intact RNA while preserving morphology, thus being suitable both for identification of specific cells and for analysis of their gene expression. The method is applicable to both mouse embryos and human tumors and may improve the preparation of cDNA libraries from specific cell types without interfering with histological diagnosis. (c)2001 Elsevier Science.
- Published
- 2002