101. Differential sensitivity of antigen- and mitogen-stimulated human leucocytes to prolonged inhibition of potassium transport
- Author
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Pamela Wright, M.R. Quastel, and J.G. Kaplan
- Subjects
biology ,Pokeweed mitogen ,Stimulation ,Cell Biology ,Molecular biology ,In vitro ,Ouabain ,chemistry.chemical_compound ,chemistry ,Antigen ,Biochemistry ,Concanavalin A ,biology.protein ,medicine ,Thymidine ,Phytohaemagglutinin ,medicine.drug - Abstract
Suspensions of human peripheral leucocytes, stimulated to undergo DNA and RNA synthesis by exposure in vitro to a variety of specific antigens, were highly sensitive to inhibition by 2 × 10 −7 M ouabain; so also were cells stimulated by the non-specific mitogens concanavalin A, pokeweed mitogen and periodate. As had previously been found for stimulation by phytohaemagglutinin (PHA), inhibition of the mitogen-stimulated cultures was freely reversible, disappearing upon resuspension of the washed cells in fresh medium. Exposure of the cultures to this concentration of ouabain for two days or more rendered them irreversibly incapable of subsequent response to all of the specific antigens tried; yet such cells remained fully able to respond to the mitogens. Suspensions subjected to high concentrations of ouabain (8 × 10 −3 M) for two days became incapable of subsequent response to PHA but could be rescued by inclusion of 20 mM K + in the medium following washing out the ouabain; such cells did not regain the ability to respond to specific antigens.
- Published
- 1973
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