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Abstract The doubly fed induction generator (DFIG) usually experiences high rotor current and DC capacitor link voltage spikes during system fault events. In this paper, a novel data‐driven approach is proposed to enhance DFIG performance under fault scenarios. An advanced reinforcement learning algorithm called guided surrogate‐gradient‐based evolution strategy (GSES) is used to control the DFIG power and capacitor DC‐link voltage by adjusting the optimal reference signals. This controller is able to prevent the DFIG rotor from over‐current risk and maintain grid‐connected operation. The proposed GSES‐based control algorithm was evaluated through simulations on a 3.6‐MW DFIG in the PSCAD/EMTDC software. Results have validated the effectiveness of the proposed GSES‐based control algorithm in improving DFIG performance under various fault scenarios.

Replacing traditional journals with a more modern solution is not a new idea. Here, we propose ways to overcome the social dilemma underlying the decades of inaction. Any solution needs to not only resolve the current problems but also be capable of preventing takeover by corporations: it needs to replace traditional journals with a decentralized, resilient, evolvable network that is interconnected by open standards and open-source norms under the governance of the scholarly community. It needs to replace the monopolies connected to journals with a genuine, functioning and well-regulated market. In this new market, substitutable service providers compete and innovate according to the conditions of the scholarly community, avoiding sustained vendor lock-in. Therefore, a standards body needs to form under the governance of the scholarly community to allow the development of open scholarly infrastructures servicing the entire research workflow. We propose a redirection of money from legacy publishers to the new network by funding bodies broadening their minimal infrastructure requirements at recipient institutions to include modern infrastructure components replacing and complementing journal functionalities. Such updated eligibility criteria by funding agencies would help realign the financial incentives for recipient institutions with public and scholarly interest.

Culex pipiens s.s./Culex torrentium belong to the most widespread mosquito taxa in Europe and are the main vectors of Sindbis, West Nile and Usutu virus. The adult overwintering females can act as reservoir for these arthropod-borne viruses (arboviruses), thus contributing to their local persistence when transmission cycles are interrupted during the winter. However, the main overwintering sites of Cx. torrentium are unknown.In a study from 2017, 3455 Cx. pipiens s.s./Cx. torrentium specimens were collected from abandoned animal burrows in Poznan, Poland. These specimens were retrospectively identified to species-level with a PCR assay, which revealed Cx. torrentium as dominant species (> 60%). Motivated by these results, we conducted a field study from February to July 2022 to systematically analyse the overwintering site patterns of Cx. pipiens s.s./Cx. torrentium. Mosquitoes were sampled using pipe traps in abandoned animal burrows (n = 20) and with aspirators in nearby anthropogenic overwintering sites (n = 23). All Cx. pipiens s.s./Cx. torrentium were screened for Flaviviridae RNA.In total, 4710 mosquitoes of five different taxa were collected from anthropogenic sites. 3977 of them were identified as Cx. p. pipiens/Cx. torrentium (Cx. p. pipiens: 85%, Cx torrentium: 1%, pools with both species: 14%). In contrast, only Cx. p. pipiens/Cx. torrentium (1688 specimens) were collected from animal burrows dominated by Cx. torrentium (52%), followed by pools with both species (40%) and Cx. p pipiens (8%). A single pool of 10 Cx. torrentium specimens collected from an animal burrow was positive for Usutu virus.The detection of Usutu virus demonstrates that Cx. torrentium can act as winter reservoir for arboviruses. Abandoned animal burrows may by the primary overwintering site for the species and should be considered in future surveillance programmes, when sampling overwintering mosquitoes.

There is a large demand for aluminum alloy assembly hole machining in aerospace manufacturing industry. The assembly hole is mainly realized by drilling, and there is a problem that the aluminum alloy exit burr is too large in the processing. The exit burr directly affects the accuracy, fatigue strength and assembly performance of the workpiece. The deburring process will greatly increase the man hour and cost. Therefore, it is necessary to study the exit burr of aluminum alloy assembly hole drilling to realize the control of exit burr. This paper systematically discusses the types, measurement methods, formation mechanism, height prediction and gross control methods of exit burr in aluminum alloy drilling, in order to provide help for the research of exit burr in aluminum alloy drilling.

Abstract Elevated plasma concentrations of asymmetric dimethylarginine (ADMA) are associated with an increased risk of mortality and adverse cardiovascular outcomes. ADMA can be metabolized by dimethylarginine dimethylaminohydrolases (DDAHs) and by alanine-glyoxylate aminotransferase 2 (AGXT2). Deletion of DDAH1 in mice leads to elevation of ADMA in plasma and increase in blood pressure, while overexpression of human DDAH1 is associated with a lower plasma ADMA concentration and protective cardiovascular effects. The possible role of alternative metabolism of ADMA by AGXT2 remains to be elucidated. The goal of the current study was to test the hypothesis that transgenic overexpression of AGXT2 leads to lowering of plasma levels of ADMA and protection from vascular damage in the setting of DDAH1 deficiency. We generated transgenic mice (TG) with ubiquitous overexpression of AGXT2. qPCR and Western Blot confirmed the expression of the transgene. Systemic ADMA levels were decreased by 15% in TG mice. In comparison with wild type animals plasma levels of asymmetric dimethylguanidino valeric acid (ADGV), the AGXT2 associated metabolite of ADMA, were six times higher. We crossed AGXT2 TG mice with DDAH1 knockout mice and observed that upregulation of AGXT2 lowers plasma ADMA and pulse pressure and protects the mice from endothelial dysfunction and adverse aortic remodeling. Upregulation of AGXT2 led to lowering of ADMA levels and protection from ADMA-induced vascular damage in the setting of DDAH1 deficiency. This is especially important, because all the efforts to develop pharmacological ADMA-lowering interventions by means of upregulation of DDAHs have been unsuccessful.

Abstract Inter‐area oscillation is a serious problem that threatens a power system. Appropriate damping control of the inter‐area oscillation would ensure the grid stability and maintain the tie‐line power transfer capability. In this paper, a novel reinforcement learning (RL) based power oscillation damping (POD) controller is proposed that uses Thyristor Controlled Series Compensators (TCSC) to damp inter‐area oscillations. By leveraging the unbiased gradient direction estimation of the natural evolution strategy (NES), the power flows on the tie‐lines were successfully regulated and inter‐area oscillations were damped through dynamically modulating the inserted reactance of the TCSC. Furthermore, parallel computation techniques were adopted to speed up the training process of the NES. The proposed RL‐based POD controller has been tested on both two‐area four‐machine system and North American MinniWECC system. Extensive studies have demonstrated the excellent performance of the proposed RL‐based TCSC POD controller in damping inter‐area oscillations.

The improvement of the surface quality of complex structural parts is a major challenge. Electrochemical mechanical polishing (ECMP) plays an important role in the polishing of wafers due to its high material removal rate. However, electrochemical mechanical polishing of the surface of complex structures, such as the bevels of turbine blades, has been little investigated. This paper proposes a new method, moist particle electrolyte electrochemical mechanical polishing (MPE-ECMP), for polishing the surfaces of complex structural parts. Using a sulfonated cation exchange resin as a moist particle electrolyte (MPE), polarization curves are measured using linear scanning voltammetry (LSV) and a selected potential of 1.0 V vs. Hg|Hg2SO4 over a 1 h polishing period. The elemental changes on the copper surface are examined by energy dispersive spectroscopy (EDS) and those on the MPE surface are studied using X-ray photoelectron spectrometry (XPS) to explain the material removal mechanism. The results show that the surface roughness of copper is reduced from the initial Sa = 433.48 nm to Sa = 21.83 nm, a reduction of 95.0%, that no copper oxides are generated during the MPE-ECMP process and that the MPE adsorbs released Cu2+. It is demonstrated that MPE-ECMP has good surface finishing properties for beveled surfaces, and provides an effective approach for surface polishing of complex structural parts.

Objective: A number of epidemiological studies have demonstrated that smoking initiation and alcohol and coffee consumption were closely related to women’s reproductive health. However, there was still insufficient evidence supporting their direct causality effect.Methods: We utilized two-sample Mendelian randomization (TSMR) analysis with summary datasets from genome-wide association study (GWAS) to investigate the causal relationship between smoking initiation, alcohol and coffee consumption, and women’s reproductive health-related traits. Exposure genetic instruments were used as variants significantly related to traits. The inverse-variance weighted (IVW) method was used as the main analysis approach, and we also performed MR-PRESSO, MR-Egger, weighted median, and weighted mode to supplement the sensitivity test. Then, the horizontal pleiotropy was detected by using MRE intercept and MR-PRESSO methods, and the heterogeneity was assessed using Cochran’s Q statistics.Results: We found evidence that smoking women showed a significant inverse causal association with the sex hormone-binding globulin (SHBG) levels (corrected β = −0.033, p = 9.05E-06) and age at menopause (corrected β = −0.477, p = 6.60E-09) and a potential positive correlation with the total testosterone (TT) levels (corrected β = 0.033, p = 1.01E-02). In addition, there was suggestive evidence for the alcohol drinking effect on the elevated TT levels (corrected β = 0.117, p = 5.93E-03) and earlier age at menopause (corrected β = −0.502, p = 4.14E-02) among women, while coffee consumption might decrease the female SHBG levels (corrected β = −0.034, p = 1.33E-03).Conclusion: Our findings suggested that smoking in women significantly decreased their SHBG concentration, promoted earlier menopause, and possibly reduced the TT levels. Alcohol drinking had a potential effect on female higher TT levels and earlier menopause, while coffee consumption might lead to lower female SHBG levels.

Objectives:Several studies have indicated a potential association between early life course-related traits and neurological and psychiatric disorders in adulthood, but the causal link remains unclear.Methods:Instrumental variables (IVs) that have been shown to be strongly associated with exposure were obtained from summary data of genome-wide association studies (GWASs). Four early life course-related traits [i.e., birthweight (BW), childhood body mass index (BMI), early body size, and age at first birth (AFB)] were used as exposure IVs to estimate their causal associations with three neurological and psychiatric diseases [i.e., Alzheimer’s disease (AD), major depressive disorder (MDD), and attention-deficit hyperactivity disorder (ADHD)]. Four different statistical methods, i.e., inverse-variance weighting (IVW), MR–Egger (MRE), weighted median (WM), and weighted mode (Wm), were performed in our MR analysis. Sensitivity analysis was performed by using the leave-one-out method, and horizontal pleiotropy was assessed using the MR-PRESSO package.Results:There was evidence suggesting that BW has a causal effect on AD (ORMR-PRESSO = 1.05, p = 1.14E-03), but this association was not confirmed via multivariable Mendelian randomization (MVMR) (ORMVMR = 0.97, 95% CI 0.92–1.02, p = 3.00E-01). A strong relationship was observed between childhood BMI and ADHD among both sexes; a 1-SD increase in BMI significantly predicted a 1.46-fold increase in the OR for ADHD (p = 9.13E-06). In addition, a similar relationship was found between early life body size and ADHD (ORMR-PRESSO = 1.47, p = 9.62E-05), and this effect was mainly driven by male participants (ORMR-PRESSO = 1.50, p = 1.28E-3). Earlier AFB could significantly predict a higher risk of MDD (ORMR-PRESSO = 1.19, p = 1.96E-10) and ADHD (ORMR-PRESSO = 1.45, p = 1.47E-15). No significant causal associations were observed between the remaining exposures and outcomes.Conclusion:Our results reveal the adverse effects of childhood obesity and preterm birth on the risk of ADHD later in life. The results of MVMR also show that lower BW may have no direct relationship with AD after adjusting for BMI. Furthermore, AFB may predict a higher risk of MDD.

The CRISPR/Cas9 system, originally derived from the prokaryotic adaptive immune system, has been developed as efficient genome editing tools. It enables precise gene manipulation on chromosomal DNA through the specific binding of programmable sgRNA to target DNA, and the Cas9 protein, which has endonuclease activity, will cut a double strand break at specific locus. However, Cas9 is a foreign protein in mammalian cells, and the potential risks associated with its introduction into mammalian cells are not fully understood. In this study, we performed pull-down and mass spectrometry (MS) analysis of Streptococcus pyogenes Cas9 (SpyCas9) interacting proteins in HEK293T cells and showed that the majority of Cas9-associated proteins identified by MS were localized in the nucleolus. Interestingly, we further discovered that the Cas9 protein contains a sequence encoding a nucleolus detention signal (NoDS). Compared with wild-type (WT) Cas9, NoDS-mutated variants of Cas9 (mCas9) are less stable, although their gene editing activity is minimally affected. Overexpression of WT Cas9, but not mCas9, causes general effects on transcription and protein translation in the host cell. Overall, identification of NoDS in Cas9 will improve the understanding of Cas9's biological function in vivo, and the removal of NoDS in Cas9 may enhance its safety for future clinical use.

Abstract Observability and detectability analyses are often used to guide the measurement setup and select the estimation models used in dynamic state estimation (DSE). Yet, marginally observable states of a synchronous machine prevent the direct application of conventional observability and detectability analyses in determining the existence of a DSE observer. To address this issue, the authors propose to identify the marginally observable states and their associate eigenvalues by finding the smallest perturbation matrices that make the system unobservable. The proposed method extends the observability and detectability analyses to marginally observable estimation models, often encountered in the DSE of a synchronous machine. The effectiveness and application of the proposed method are illustrated on the IEEE 10‐machine 39‐bus system, verified using the unscented Kalman filter and the extended Kalman filter, and compared with conventional methods. The proposed analysis method can be applied to guide the selection of estimation models and measurements to determine the existence of a DSE observer in power‐system planning.

Abstract Linear time‐varying (LTV) systems play a key role in many engineering problems, for example, trajectory sensitivity analysis of power systems. Because LTV systems do not generally have closed‐form solutions, estimates of their bounds are crucial to their analysis. In this paper, bounds for LTV systems with known inputs and unknown but bounded inputs are obtained via Grönwall's inequality. The estimated bounds are then used to quantify the gap between synchronous generators and their models in different scenarios through simulation.

Shape memory alloys (SMAs) exhibit a unique property that undergoes deformation in response to temperature variation. This characteristic can be utilized via the application of a filiform SMA wire to tendon-driven robotic actuators for biomimetic joint movements. However, due to the inefficiencies in heat dissipation, conventional SMA tendon-driven actuators are characterized by their lower relaxation speeds than other actuators. This paper proposes a novel cooling design for an SMA tendon-driven actuator using thin-fin heat sinks based on a multi-layer wrapped SMA tendon design. In addition, the electric circuit and the controller are refined. Prototype devices are constructed to validate the performance of SMA-based actuators under PID control. The results indicate that the proposed design exceeds previous models in terms of relaxation performance by up to 5.8 times while also being able to stabilize at a target angle within 0.5 s under control.

Off-axis parabolic mirrors have extensive applications in X-ray optics, with the precision of their curvature directly impacting grazing-incidence focusing performance. Notably, the off-axis parabolic surface has non-rotating and non-symmetrical characteristics. Ultra-precision raster grinding utilizing a diamond wheel is a common method. Crucially, establishing an optimal wheel path stands as the key to ensuring surface accuracy during off-axis paraboloid grinding. In this study, according to the double curvature property of an off-axis parabolic surface, two different wheel paths were compared: one tracing the meridian direction (parabolic generatrix) and the other following the arc vector direction (arc). The results showed that the wheel path in raster grinding stepping along the arc vector direction can obtain a smaller scallop height and higher surface accuracy. The surface accuracy of one step along the arc vector direction is 9.6 μm, and that of the other step along the meridian direction is 32.6 μm. A model of the scallop height was established based on the relative relationship between adjacent wheel paths, and the error is within 5%. According to the correlation between scallop height and shape error, we conducted an analysis of the spatial distribution of shape errors under varying wheel paths. The wheel path that steps along the arc vector is more suitable for raster grinding of the off-axis paraboloid. The above study can provide theoretical guidance for the wheel path planning of off-axis parabolic mirrors with high surface accuracy.

Seasonal–trend-decomposed transformer has empowered long-term time series forecasting via capturing global temporal dependencies (e.g., period-based dependencies) in disentangled temporal patterns. However, existing methods design various auto-correlation or attention mechanisms in the seasonal view while ignoring the fine-grained temporal patterns in the trend view in the series decomposition component, which causes an information utilization bottleneck. To this end, a Transformer-based seasonal–trend decomposition methodology with a multi-scale attention mechanism in the trend view and a multi-view attention mechanism in the seasonal view is proposed, called DESTformer. Specifically, rather than utilizing the moving average operation in obtaining trend data, a frequency domain transform is first applied to extract seasonal (high-frequency) and trend (low-frequency) components, explicitly capturing different temporal patterns in both seasonal and trend views. For the trend component, a multi-scale attention mechanism is designed to capture fine-grained sub-trends under different receptive fields. For the seasonal component, instead of the frequency-only attention mechanism, a multi-view frequency domain (i.e., frequency, amplitude, and phase) attention mechanism is designed to enhance the ability to capture the complex periodic changes. Extensive experiments are conducted on six benchmark datasets covering five practical applications: energy, transportation, economics, weather, and disease. Compared to the state-of-the-art FEDformer, our model shows reduced MSE and MAE by averages of 6.5% and 3.7%, respectively. Such experimental results verify the effectiveness of our method and point out a new way towards handling trends and seasonal patterns in long-term time series forecasting tasks.

Segesterone acetate (SA) or Nestorone, a fourth-generation progestogen, is a synthetic compound with high progestational activity and no androgenic, glucocorticoid, or anabolic effects. However, due to its oral inactivity, SA must be used by other routes, such as subcutaneous. Thus, considering its peculiar properties, the SA subdermal implant is successfully used in female contraception and postmenopausal hormone replacement therapy (HRT). In recent years, its potential uses in endometriosis, polycystic ovaries syndrome (PCOS), and a new therapeutic possibility for neuroprotection have made this treatment extremely interesting. However, the absence of a standardized dose and the long-term safety of SA implant therapy in women is still controversial. Here, we present the possible indications, doses, limitations, and side effects of SA implant therapy.

SiC fiber reinforced SiC ceramic matrix composites (SiCf/SiC) have problems such as poor machining quality and difficult material removal. To study these problems, SiCf/SiC composites were processed by ultrasonic-assisted helical grinding. The exit quality of hole, hole wall morphology and three-dimensional surface roughness of hole wall were explored. The results show that compared with traditional hole making, the material at the exit of the hole made by ultrasonic-assisted helical grinding has no large area chipping. The periodic change of the angle θ between the direction of the cutting speed of the grinding wheel and the fiber direction leads to regular changes in the surface morphology of the hole wall. When θ is 0°/180°, the fiber and the matrix are debonded. When θ is 45°, the fiber is sheared and fractured. When θ is 90°, the fiber is crushed and fractured. When θ is 135°, the fiber has both shear fracture and extrusion fracture. The hole wall surface roughness Sa is the lowest value when θ is 90°, and Sa is the highest value when θ is 135°. Within a certain range, the surface roughness value Sa decreases with the increase of the ultrasonic amplitude, with a maximum decrease of 38.7%, and increases with the increase of the feed rate, with a maximum increase of 39.3%.

Comorbidity of pain and depression is a common health problem that seriously affects the physical and psychological health of patients. However, the pathogenesis of these symptoms remains unclear. To comprehensively understand the mechanisms and treatments of pain and depression comorbidity, this review summarizes the establishment of related animal models and behavioral evaluation methods of pain and depression comorbidity at home and abroad.

Effective detection of small targets plays a pivotal role in infrared (IR) search and track applications for modern military defense or attack. However, IR small targets are very difficult to detect because of their weak brightness, small size, and lack of shape, structure, texture, and other information elements. In order to simultaneously satisfy the robustness and timeliness of target detection, inspired by density peak clustering and the human visual system, an idea combining an improved density peak global search and local contrast calculation is proposed. First, the positions of candidate targets are determined in the preprocessed image using the improved density peak global search method (IDPGSM). Second, the saliency map is obtained using the double-weights enhanced local contrast method (DWELCM) for the candidate target neighborhood. Finally, adaptive threshold segmentation is used to detect IR small targets. Through comprehensive analysis of five evaluation indicators, the experimental results on seven real sequences and three hundred IR images of different scenes that the proposed method has better detection performance compared with six baseline methods. It can quickly and accurately determine the small target position in the case of severe background clutter and noise interference.

Abstract Background In patients with type 2 diabetes (T2D) sodium-glucose cotransporter 2 (SGLT-2) inhibitors improve glycaemic control as well as cardiovascular and renal outcomes. Their effects on l-arginine (Arg) related risk markers asymmetric and symmetric dimethylarginine (ADMA and SDMA) and the protective biomarker L-homoarginine (hArg) linking T2D to cardiovascular and renal disease have not yet been reported. Methods Plasma and 24-h urine samples taken before and after 6 weeks of treatment were available from two prospective, randomized, double-blind, placebo-controlled, cross-over trials with empagliflozin (71 patients analyzed, NCT02471963) and dapagliflozin (59 patients analyzed, NCT02383238). In these samples, concentrations of hArg, Arg, ADMA, SDMA, and creatinine were determined by liquid-chromatography coupled to tandem mass-spectrometry. Additionally, intraindividual changes of the biomarkers in plasma were correlated with intraindividual changes of clinical parameters. Results Treatment with empagliflozin and dapagliflozin was associated with a reduction of plasma hArg by 17.5% and 13.7% (both p

Abstract Background The invasive species Aedes albopictus, commonly known as the Asian tiger mosquito, has undergone extreme range expansion by means of steady introductions as blind passengers in vehicles traveling from the Mediterranean to south-west Germany. The more than 25 established populations in the State of Baden-Württemberg, Palatine and Hesse (south-west Germany) have become a major nuisance and public health threat. Aedes albopictus deserves special attention as a vector of arboviruses, including dengue, chikungunya and Zika viruses. In Germany, Ae. albopictus control programs are implemented by local communities under the auspices of health departments and regulatory offices. Methods The control strategy comprised three pillars: (i) community participation (CP) based on the elimination of breeding sites or improved environmental sanitation, using fizzy tablets based on Bacillus thuringiensis israelensis (fizzy Bti tablets; Culinex® Tab plus); (ii) door-to-door (DtD) control by trained staff through the application of high doses of a water-dispersible Bti granular formulation (Vectobac® WG) aimed at achieving a long-lasting killing effect; and (iii) implementation of the sterile insect technique (SIT) to eliminate remaining Ae. albopictus populations. Prior to initiating large-scale city-wide treatments on a routine basis, the efficacy of the three elements was evaluated in laboratory and semi-field trials. Special emphasis was given to the mass release of sterile Ae. albopictus males. Results More than 60% of the local residents actively participated in the first pillar (CP) of the large-scale control program. The most effective element of the program was found to be the DtD intervention, including the application of Vectobac® WG (3000 ITU/mg) to potential breeding sites (10 g per rainwater container, maximum of 200 l = maximum of approx. 150,000 ITU/l, and 2.5 g per container

Alcohol ranks as one of the leading behavioral threats to health and life in developed countries. Alcohol abuse triggers serious social and economic effects: it contributes to higher prevalence of work-related and road accidents, as well as absence from work. The diagnosis and treatment of alcoholism still remain very difficult. Hence, the use of objective biochemical markers of alcohol abuse may contribute to earlier detection, more effective therapy and reliable teetotalism control. The aim of this study is to present the sensitive and specific biomarkers of alcohol abuse available in Poland, with particular emphasis on the practical use possibilities. Such tests may be widely used, e.g., in driving license regranting cases involving drivers whose licenses were suspended for driving when intoxicated, for the early detection of persons abusing alcohol in employment-related health controls, for abstinence monitoring during withdrawal treatment, for detecting alcohol consumption in transplant settings, for assessing the prevalence of alcohol drinking in pregnancy, as well as in autopsical examinations. The standardization of biomarkers measurement methods is essential. Moreover, concomitant disorders may pose a significant problem in the proper outcome analysis. Despite these limitations, objective biochemical markers of ethyl alcohol abuse may become helpful tools in medical care. They can play a particular role in occupational medicine diagnostics, contributing to the higher level of safety on public roads and to worker safety. Med Pr. 2021;72(2):173–84

Immune cells of myeloid origin, including microglia, macrophages, and myeloid-derived suppressor cells adopt immunosuppressive phenotypes that support gliomagenesis. Here, we tested an a priori hypothesis that single nucleotide polymorphisms (SNPs) in genes related to glioma-associated myeloid cell regulation and function are also associated with patient survival after glioma diagnosis. Subjects for this study were 992 glioma patients treated at The University of Texas MD Anderson Cancer Center in Houston, Texas between 1992 and 2008. Haplotype-tagging SNPs in 91 myeloid-associated genes were analyzed for association with survival by Cox regression. Individual SNP- and gene-based tests were performed separately in glioblastoma (WHO grade IV, n = 511) and lower-grade glioma (WHO grade II-III, n = 481) groups. After adjustment for multiple testing, no myeloid-associated gene variants were significantly associated with survival in glioblastoma. Two SNPs, rs147960238 in CD163 (p = 2.2 × 10-5) and rs17138945 in MET (p = 5.6 × 10-5) were significantly associated with survival of patients with lower-grade glioma. However, these associations were not confirmed in an independent analysis of 563 lower-grade glioma cases from the University of California at San Francisco Adult Glioma Study (p = 0.65 and p = 0.41, respectively). The results of this study do not support a role for inherited polymorphisms in myeloid-associated genes in affecting survival of patients diagnosed with glioblastoma or lower-grade glioma.

Abstract Background In Europe, Aedes albopictus is an important vector of chikungunya virus and Dirofilaria nematodes and has been involved in local autochthonous circulation of dengue and Zika viruses. Due to the ongoing spread, targeted field surveillance at potential points of entry of invasive Aedes mosquitoes was initiated by the Republic of Moldova in 2020 as part of the transboundary “Invasive Aedes Mosquitoes COST-Action project.” Methods In 2020, ovitraps were positioned at each of three locations: the border crossing to Romania in Leuşeni (Hancesti region), Chişinӑu International Airport and Chişinӑu Botanical Garden. Results A total of 188 Aedes spp. eggs were collected at the Chişinӑu International Airport between August and September 2020. Twenty-three adults reared in the laboratory were identified morphologically as Ae. albopictus (Skuse, 1895), and 12 selected specimens were confirmed by molecular barcoding of the cytochrome oxidase subunit I gene region. In addition, one adult Ae. albopictus female at the same site was caught with a manual aspirator. Conclusions This is the first documented report of Ae. albopictus in the Republic of Moldova. The presence of immature and adult stages indicates the local reproduction of the species in the country. Therefore, it is crucial to extend and strengthen surveillance of the invasive Aedes mosquitoes to prevent Ae. albopictus and other exotic mosquito species from becoming established in the Republic of Moldova. Graphical abstract

Haff disease is a group of symptoms caused by rhabdomyolysis following ingestion of some species of fish and crayfish. Pathophysiology remains unknown. Outbreaks of the Haff disease have been reported in many regions of the world. In this article we present the case of a 38-years-old patient, professional fisherman, suspected of suffering from Haff disease. He developed symptoms of diffuse myalgia, headache, chest pressure, brown-colored urine and elevated blood pressure. Predominant laboratory abnormalities were elevated serum creatine kinase and creatinine concentrations. During hospitalization the patient required the renal replacement therapy. As a result of applied procedures, clinical and laboratory improvement was achieved. The patient was discharged home in good general condition. The Haff disease should be included in the differential diagnosis of rhabdomyolysis. Detailed medical interview, especially questions about recently consumed food and occupational exposure, is crucial in making a proper diagnosis. Med Pr. 2021;72(5):605–10

Abstract Background Inflammatory osteolysis, a major complication of total joint replacement surgery, can cause prosthesis failure and necessitate revision surgery. Macrophages are key effector immune cells in inflammatory responses, but excessive M1-polarization of dysfunctional macrophages leads to the secretion of proinflammatory cytokines and severe loss of bone tissue. Here, we report the development of macrophage-biomimetic porous SiO2-coated ultrasmall Se particles (porous Se@SiO2 nanospheres) to manage inflammatory osteolysis. Results Macrophage membrane-coated porous Se@SiO2 nanospheres(M-Se@SiO2) attenuated lipopolysaccharide (LPS)-induced inflammatory osteolysis via a dual-immunomodulatory effect. As macrophage membrane decoys, these nanoparticles reduced endotoxin levels and neutralized proinflammatory cytokines. Moreover, the release of Se could induce macrophage polarization toward the anti-inflammatory M2-phenotype. These effects were mediated via the inhibition of p65, p38, and extracellular signal-regulated kinase (ERK) signaling. Additionally, the immune environment created by M-Se@SiO2 reduced the inhibition of osteogenic differentiation caused by proinflammation cytokines, as confirmed through in vitro and in vivo experiments. Conclusion Our findings suggest that M-Se@SiO2 have an immunomodulatory role in LPS-induced inflammation and bone remodeling, which demonstrates that M-Se@SiO2 are a promising engineered nanoplatform for the treatment of osteolysis occurring after arthroplasty. Graphical Abstract

Abstract Background For over a decade, monitoring of West Nile virus (WNV) in Germany has consisted of a bird monitoring programme as well as a mosquito-based surveillance programme employing CO2-baited encephalitis vector surveillance (EVS) traps for mass trapping and screening of mosquitoes. In contrast to the EVS traps, the Reiter/Cummings type box gravid trap collects gravid female mosquitoes, which have already taken a blood meal, increasing the likelihood of being infected with pathogens. The traps can be equipped with a honey-baited Flinders Technology Associates® (FTA) card to encourage sugar feeding by the trapped mosquitoes. FTA cards contain nucleic acid preserving substances, which prevent the degradation of viral RNA in the expectorated mosquito saliva and allows for testing the card for flavivirus RNA. This study aimed to assess the suitability of the method for WNV surveillance in Germany as an alternative to previous methods, which are expensive, time-consuming, and predominantly target host-seeking populations less likely to be infected with WNV. Methods In the Thüringer Zoopark Erfurt, snowy owls (Nyctea scandiaca) and greater flamingos (Phoenicopterus roseus) died of WNV infections in July and August 2020. In response, five Reiter/Cummings type box gravid traps were positioned during the daytime on the 10th, 13th, and 16th of September in five different locations. The FTA cards and mosquitoes in the chamber were collected, kept in a cool chain, and further processed for virus detection using a modified generic flavivirus reverse transcription PCR. Results A total of 15 trappings during September collected a total of 259 female mosquitoes, 97% of which were Culex pipiens sensu lato, as well as 14 honey-baited FTA cards. Eight mosquitoes tested PCR-positive for WNV. Four FTA cards tested PCR-positive for mosquito-borne flaviviruses, two of which were confirmed as WNV, and the remaining two confirmed as Usutu virus. Conclusion The suitability of the FTA cards in preserving viral RNA in the field and rapid turnaround time from collection to result is combined with a simple, cost-effective, and highly specific trapping method to create an arbovirus surveillance system, which circumvents many of the difficulties of previous surveillance programmes that required the analysis of mosquitoes in the laboratory. Graphical Abstract