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Made available in DSpace on 2022-04-28T19:53:56Z (GMT). No. of bitstreams: 0 Previous issue date: 1993-01-01 Spatial patterns of morphometric variation in Apis cerana indica were analysed. Factor and spatial autocorrelation analyses were applied to 29 characters, measured in 17 populations in India. Correlograms showed that 15 characters are patterned geographically, and 13 of them are related to overall size. These characters are distributed as a north-south cline, probably reflecting adaptations to environmental conditions. However, the great number of characteristics without geographical pattern suggests that part of the morphometric variability is due to local stochastic divergences. © 1993 International Bee Research Association. Departamento de Biologia Institute de Biociências-UNESP, CR 199, Rio Clara, Sao Paulo, CEP: 13506-900 Departamento de Biologia Institute de Biociências-UNESP, CR 199, Rio Clara, Sao Paulo, CEP: 13506-900

The current study compared the toxicity of different concentrations of boric acid in adult workers of Atta sexdens rubropilosa Forel (Hymenoptera: Formicidae), with toxicological bioassays, and examining the dose-dependent and time-dependent histopathological changes, of the midgut, Malpighian tubules, and postpharyngeal glands. Our results revealed the importance of conducting toxicological bioassays combined with morphological analyses of the organs of ants chronically exposed to insecticides used in commercial ant baits. In vitro bioassays showed that boric acid significantly decreases the survivorship of workers regardless of concentration, whereas the morphological data suggested progressive dose-dependent and time-dependent changes in the organs examined, which were evident in the midgut. The midgut is the first organ to be affected, followed by the postpharyngeal gland and Malpighian tubules. This sequence is in agreement with the absorption pathway of this chemical compound in the midgut, its transference to the hemolymph, possibly reaching the postpharyngeal glands, and excretion by the Malpighian tubules. These progressive changes might be due to the cumulative and delayed effect of boric acid. Our findings provide important information for the understanding of the action of boric acid in ant baits in direct and indirect target organs.

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T18:44:49Z No. of bitstreams: 1 S0073-47212009000100009.pdf: 46920 bytes, checksum: 241a41d87490ab638905513ce7e82c47 (MD5) Made available in DSpace on 2013-08-22T18:44:49Z (GMT). No. of bitstreams: 1 S0073-47212009000100009.pdf: 46920 bytes, checksum: 241a41d87490ab638905513ce7e82c47 (MD5) Previous issue date: 2009-03-01 Made available in DSpace on 2013-09-30T19:33:17Z (GMT). No. of bitstreams: 2 S0073-47212009000100009.pdf: 46920 bytes, checksum: 241a41d87490ab638905513ce7e82c47 (MD5) S0073-47212009000100009.pdf.txt: 26711 bytes, checksum: 87d6cbb1a53a6dd9056cb5db31f2cc17 (MD5) Previous issue date: 2009-03-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T15:09:06Z No. of bitstreams: 2 S0073-47212009000100009.pdf: 46920 bytes, checksum: 241a41d87490ab638905513ce7e82c47 (MD5) S0073-47212009000100009.pdf.txt: 26711 bytes, checksum: 87d6cbb1a53a6dd9056cb5db31f2cc17 (MD5) Made available in DSpace on 2014-05-20T15:09:06Z (GMT). No. of bitstreams: 2 S0073-47212009000100009.pdf: 46920 bytes, checksum: 241a41d87490ab638905513ce7e82c47 (MD5) S0073-47212009000100009.pdf.txt: 26711 bytes, checksum: 87d6cbb1a53a6dd9056cb5db31f2cc17 (MD5) Previous issue date: 2009-03-01 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Muitas substâncias de origem vegetal podem ser tóxicas ou apresentar potencial inseticida. Com o objetivo de diminuir a problemática da poluição ambiental alguns estudos vêm tentando substituir os inseticidas artificiais pelos inseticidas botânicos. Ricinus communis (Euphorbiaceae) apresenta uma grande variedade de substâncias sendo a ricinina o principal componente tóxico. Considerando que as abelhas são insetos benéficos por atuarem como agentes polinizadores das plantas, este estudo teve por objetivo avaliar o efeito tóxico da ricinina para as operárias e larvas de Apis mellifera (Linnaeus, 1758) (Hymenoptera, Apidae) e Scaptotrigona postica (Latreille, 1907) (Hymenoptera, Meliponini). Para isso, foram realizados testes de ingestão em operárias confinadas recebendo ricinina incorporada à dieta e testes de aplicação tópica com a substância solubilizada em metanol e aplicada no pronoto das abelhas com auxílio de uma microseringa. Para as larvas foram realizados testes de ingestão e calculada sua taxa de mortalidade. Os resultados mostram atividade tóxica significativa (p < 0.0001) da ricinina nas abelhas adultas das duas espécies para a concentração 0,1% nos testes de ingestão e 0,2% nos testes de aplicação tópica. Outrossim, uma elevada porcentagem de larvas foi afetada negativamente pela ricinina logo nos primeiros dias de vida para todas as concentrações testadas (0,25%, 0,5% e 1%). Many substances of vegetal origin can be toxic or present an insecticidal potential. With the aim of decreasing the environment pollution problem, a few studies are trying to substitute synthetic insecticides with botanical ones. Ricinus communis (Euphorbiaceae) presents a great variety of substances, being the ricinine the main toxic component. Considering that bees are useful as pollinator agents of plants, this study evaluates toxicity potential of ricinine on workers and larvae of Apis mellifera (Linnaeus, 1758) (Hymenoptera, Apidae) and Scaptotrigona postica (Latreille, 1907) (Hymenoptera, Meliponini). In order to determine ricinine toxicity, ingestion tests were carried out with isolated workers bees that received ricinine on its diet. Furthermore, for topic tests, solutions of ricinine in methanol were applied on pronotum of worker bees with an Agla brand micrometer syringe outfit. For larvae of bees, ingestion tests were used and mortality rates were calculated. According to the results, it was detected a significant toxic activity (p < 0.0001) of ricinine on the workers of the two bee species at concentrations of 0.1% in the ingestion tests and 0.2% in the topic tests. For all concentrations (0.25%, 0.5% and 1%), a high percentage of larvae bees was negatively affected by ricinine on the first days of life. Universidade Estadual Paulista Instituto de Biociências Departamento de Botânica Universidade Estadual Paulista Instituto de Biociências Departamento de Biologia Universidade Federal de São Carlos (UFSCar) Centro de Ciências Exatas e de Tecnologia Departamento de Química Universidade Estadual Paulista Instituto de Biociências Departamento de Botânica Universidade Estadual Paulista Instituto de Biociências Departamento de Biologia

The protein complement of the secretion from hypopharyngeal gland of nurse-bees (Apis mellifera L.) was partially identified by using a combination of 2D-PAGE, peptide sequencing by MALDI-PSD/MS and a protein engine identification tool applied to the honeybee genome. The proteins identified were compared to those proteins already identified in the proteome complement of the royal jelly of the honey bees. The 2-D gel electrophoresis demonstrated this protein complement is constituted of 61 different polypepides, from which 34 were identified as follows: 27 proteins belonged to MRJPs family, 5 proteins were related to the metabolism of carbohydrates and to the oxido-reduction metabolism of energetic substrates, 1 protein was related to the accumulation of iron in honeybee bodies and 1 protein may be a regulator of MRJP-1 oligomerization. The proteins directly involved with the carbohydrates and energetic metabolisms were: alpha glucosidase, glucose oxidase and alpha amylase, whose are members of the same family of enzymes, catalyzing the hydrolysis of the glucosidic linkages of starch; alcohol dehydrogenase and aldehyde dehydrogenase, whose are constituents of the energetic metabolism. The results of the present manuscript support the hypothesis that the most of these proteins are produced in the hypoharyngeal gland of nurse-bees and secreted into the RJ.

Astilbin was isolated in high yield from Dimorphandra mollis, and its insecticidal and growth inhibiting activity by stomach ingestion were evaluated against Anticarsia gemmatalis and Spodoptera frugiperda. The insecticidal activity of astilbin, the weight reduction of the larval phase and the prolongation of the larval and pupal phases were verified for both species. Astilbin was identified on the base of its NMR, MS and physical data.

Desenvolveu-se método para determinação de Heptacloro, Aldrin, Dieldrin e Endrin em própolis (sólida e solução). Após homogeneização da amostra com Celite, a extração dos pesticidas foi efetuada com hexano e o extrato purificado em cartucho de Florisil. A eluição dos pesticidas foi realizada com hexano:diclorometano (9:1 v/v) e o eluato concentrado e analisado por cromatografia em fase gasosa com detecção por captura de elétrons (CG–DCE). A eficiência do método proposto foi demonstrada pelos valores médios de recuperação entre 79 e 100%, com valores de desvio padrão relativo entre 2,7 e 16,6%, obtidos nas análises de recuperação realizadas com amostras fortificadas nos níveis 0,6 e 1,2 µg/g. METHOD FOR THE DETERMINATION OF HEPTACHLOR, ALDRIN, DIELDRIN AND ENDRIN IN PROPOLIS Abstract A method for the determination of Heptachlor, Aldrin, Dieldrin and Endrin in propolis (solid and solution) was developed. After the samples homogenization with Celite the pesticides extraction was carried out with hexane and the extract was purified in Florisil cartridge. The pesticides elution was processed with hexane:dichloromethane (9:1 v/v) and the concentrated eluate was analysed using gas-liquid chromatography with electron capture detection (GC–ECD). The efficiency of the proposed method was demonstrated by mean recoveries, ranging from 79 to 100% with relative standard deviation values between 2.7 to 16.6%, obtained in the recovery analysis with fortified samples at levels of 0.6 and 1.2 µg/g

Made available in DSpace on 2021-07-14T10:38:23Z (GMT). No. of bitstreams: 0 Previous issue date: 2000 This paper analyzes the summer and winter total protein content of 7, 14, 21, 28, 35, and 40-day old Apis mellifera L. worker venom glands before (control) and 24 and 96 hours after applying electrical shocks for venom extraction (experimental). During venom extraction, 7-day old workers responded more slowly and weakly to electrical shocks. This response intensifies with age, so that the workers approaching 20 days old respond faster and more aggressively to the shocks. Statistical analysis, using the non-parametric Wilcoxon and Kruskall-Wallis tests and complemented by the Jonckheere test, showed that the protein content varied from one age to another in the experimental group, which was well distinguishable from the values in the control Group in summer and winter. Summer values at all ages were always higher than those detected in winter in both groups. This variation seems to indicate the occurrence of more than one winter glandular development cycle. Histological studies showed secretion in the lumen of the control Group secretory tubes and reservoirs. The experimental group only showed vestigial secretion in the collapsed reservoirs at all ages, except at 7 days. These workers, which reacted less efficiently to electrical shocks, showed secretion in the lumen, reservoir, and tubes, even after the application of electrical shocks. During the 96 hours following the electrical shocks, a slight protein replacement was seen at some ages. This, although higher in summer than in winter, was much lower than the level detected in the control group at all ages. The significantly lower values were frequent in the older workers 96 hours after extraction and could reflect reabsorption or degradation of proteins from glandular secretion due to aging. Our results show that venom extraction is more productive in summer using older workers. However, their capacity of replacing protein eliminated during stinging of the substrate, in response to shocks is shown to be low, as demonstrated for other analyzed bees. Federal University of Acre, Department of Natural Sciences Universidade Estadual Paulista, Department of Biology and Center for the Study of Social Insects - UNESP Universidade Estadual Paulista, Department of Biology and Center for the Study of Social Insects - UNESP

Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-27T11:18:11Z No. of bitstreams: 0 Made available in DSpace on 2014-05-27T11:18:11Z (GMT). No. of bitstreams: 0 Previous issue date: 1997-01-01 In this paper we proposed to estimate the heritability of seven morphological characters that compose the sting apparatus ofthe Africanized honeybee workers. An experimental design to estimate genetic parameters was based on the method developed by Oldroyd and Moran. This method was modified to eliminate within-colony environmental effects associated with the additive genetic variance. The estimated h2 values ranged from 0.17 ± 0.11 (maximum width of bulb of sting stylet and height of the valve of right lancet) to 0.74 ± 0.30 (length of the lancet). Departamento de Biologia Instituto de Biociencias, Caixa Postal 199, CEP 13.506-900, Rio Claro, Sao Paulo

Made available in DSpace on 2014-12-03T13:11:00Z (GMT). No. of bitstreams: 0 Previous issue date: 2013-11-13Bitstream added on 2014-12-03T13:22:50Z : No. of bitstreams: 1 WOS000327254700167.pdf: 814540 bytes, checksum: e0d75b567b3498a46b360a4289d79ce6 (MD5) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) FINEP (CT-BIOTEC-Anticorpos -MCTFINEP) Butantan Institute Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Victims of massive bee attacks become extremely ill, presenting symptoms ranging from dizziness and headache to acute renal failure and multiple organ failure that can lead to death. Previous attempts to develop specific antivenom to treat these victims have been unsuccessful. We herein report a F(ab)'(2)-based antivenom raised in horse as a potential new treatment for victims of multiple bee stings. The final product contains high specific IgG titers and is effective in neutralizing toxic effects, such as hemolysis, cytotoxicity and myotoxicity. The assessment of neutralization was revised and hemolysis, the primary toxic effect of these stings, was fully neutralized in vivo for the first time. Univ Sao Paulo, Sch Med, FMUSP, Div Clin Immunol & Allergy, Sao Paulo, Brazil Inst Invest Immunol INCT, Sao Paulo, Brazil Univ Sao Paulo, Fac Ciencias Farmaceut, Sao Paulo, Brazil Butantan Inst, Div Hyperimmune Plasmas, Sao Paulo, Brazil Univ Sao Francisco, Sch Med, BR-12900000 Braganca Paulista, SP, Brazil Butantan Inst, Special Pilot Lab Res & Dev Vet Immunobiol, Sao Paulo, Brazil Inst Technol Parana, Curitiba, Parana, Brazil Butantan Inst, Lab Biopharmaceut Anim Cells, Sao Paulo, Brazil Univ Sao Paulo, Sch Med, Heart Inst InCor, LIM 19, Sao Paulo, Brazil Univ Sao Paulo State UNESP, Ctr Study Social Insects, Inst Biosci, Dept Biol, Rio Claro, SP, Brazil Univ Sao Paulo State UNESP, Ctr Study Social Insects, Inst Biosci, Dept Biol, Rio Claro, SP, Brazil FAPESP: 11/51684-1

Made available in DSpace on 2021-07-14T10:38:01Z (GMT). No. of bitstreams: 0 Previous issue date: 1995 A comparison among the profiles of molecular exclusion chromatography in Sephadex G 100 column of venoms from Apis mellifera adansonii and Africanized honeybees revealed unique peaks which might be used to identify these populations. The venoms from hybrid populations resulting from the reciprocal mating of Apis mellifera adansonii and Africanized honeybees presented unique peaks, probably resulting from a synergistic effect between the parental genomes. The occurrence of characteristic peaks in venoms of hybrid populations might be used to identify these populations as well as to distinguish them from their parents. UNESP, Center for the Study of Venoms and Venomous Animals - CEVAP Institute of Biosciences of Rio Claro, Department of Biology UNESP, Center for the Study of Social Insects - CEIS UNESP, Center for the Study of Venoms and Venomous Animals - CEVAP UNESP, Center for the Study of Social Insects - CEIS

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Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T18:54:18Z No. of bitstreams: 1 S0101-81751989000100009.pdf: 3416849 bytes, checksum: e305d85d5c2126855fc4da4a5575d88f (MD5) Made available in DSpace on 2013-08-22T18:54:18Z (GMT). No. of bitstreams: 1 S0101-81751989000100009.pdf: 3416849 bytes, checksum: e305d85d5c2126855fc4da4a5575d88f (MD5) Previous issue date: 1989-04-01 Made available in DSpace on 2013-09-30T19:46:14Z (GMT). No. of bitstreams: 2 S0101-81751989000100009.pdf: 3416849 bytes, checksum: e305d85d5c2126855fc4da4a5575d88f (MD5) S0101-81751989000100009.pdf.txt: 25102 bytes, checksum: d8ee2ee576ad55139e9f592dbe16d643 (MD5) Previous issue date: 1989-04-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T15:12:11Z No. of bitstreams: 2 S0101-81751989000100009.pdf: 3416849 bytes, checksum: e305d85d5c2126855fc4da4a5575d88f (MD5) S0101-81751989000100009.pdf.txt: 25102 bytes, checksum: d8ee2ee576ad55139e9f592dbe16d643 (MD5) Made available in DSpace on 2014-05-20T15:12:11Z (GMT). No. of bitstreams: 2 S0101-81751989000100009.pdf: 3416849 bytes, checksum: e305d85d5c2126855fc4da4a5575d88f (MD5) S0101-81751989000100009.pdf.txt: 25102 bytes, checksum: d8ee2ee576ad55139e9f592dbe16d643 (MD5) Previous issue date: 1989-04-01 Twetve external morphological traits and seven traits of food-gathering behavior were studied in africanized and caucasian (A. m. caucasica), bees. Discriminant analysis showed that neither the length of the fourth segment of the abdomen, nor bee weight can be considered as the traits with the highest discriminatory power between africanized and caucasian bees, with any in-bred bees. In the case of in-bred bees the discriminam traits had a lower interorbital width and time to reach the feeder. Multivariate comparisons between the data of in-bred colonies and twenty hibrid colonies, suggest dominance of the genes group of the africanized subspecies. UNESP Instituto de Biociências Departamento de Biologia UNESP Instituto de Biociências Departamento de Biologia

The indiscriminate use of pesticides has become a serious environmental concern. Of them, imidacloprid (IMI) is one of the most widely used worldwide. In 2010 in Brazil, 1.934tonnes of IMI were sold and mainly used for sugarcane crops. Several studies have examined the toxicity of IMI as well as its possible ecological effects. However, few studies have examined its toxicity at the genetic level. This is one of the biggest challenges for the scientific community, which is concerned about the impacts of these contaminants on the environment and human health. In this study, we evaluated the effects of IMI above the genetic material in Allium cepa and Tradescantia pallida following exposure to different concentrations of this insecticide. The results demonstrated that the concentrations tested induced chromosomal alterations and increased the frequency of micronuclei. Therefore, IMI in these concentrations was genotoxic to the tested organisms. These factors should be taken into account when applying this pesticide.

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2014-02-26T17:05:42Z No. of bitstreams: 1 WOSA1993MP58800004.pdf: 104346 bytes, checksum: 1c59079450dd464a36077fe3e096fceb (MD5) Made available in DSpace on 2014-02-26T17:05:42Z (GMT). No. of bitstreams: 1 WOSA1993MP58800004.pdf: 104346 bytes, checksum: 1c59079450dd464a36077fe3e096fceb (MD5) Previous issue date: 1993-12-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T15:28:16Z No. of bitstreams: 1 WOSA1993MP58800004.pdf: 104346 bytes, checksum: 1c59079450dd464a36077fe3e096fceb (MD5) Made available in DSpace on 2014-05-20T15:28:16Z (GMT). No. of bitstreams: 1 WOSA1993MP58800004.pdf: 104346 bytes, checksum: 1c59079450dd464a36077fe3e096fceb (MD5) Previous issue date: 1993-12-01 Narrow and broad sense heritabilities (h(2)) of the number of ovarioles in Apis mellifera L. workers were estimated using two distinct data sets, based on groups of half (queens inseminated by several drones) and super-sisters (single drone insemination). The values found are in the usual range for economically important characters (0.25-0.38). UNESP,IBRC,DEPT BIOL,CAIXA POSTAL 199,BR-13506900 RIO CLARO,SP,BRAZIL UNESP,IBRC,DEPT BIOL,CAIXA POSTAL 199,BR-13506900 RIO CLARO,SP,BRAZIL

Made available in DSpace on 2018-11-26T15:28:30Z (GMT). No. of bitstreams: 0 Previous issue date: 2015-12-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) The bee species Melipona scutellaris Latreille, 1811 (Hymenoptera: Apidae) is native to Brazil and, stingless. In Brazil, stingless bees are responsible for 40% to 90% of tree species pollination, depending on the considered ecosystem. However, their survival has been threatened since the country has been standing out as a big consumer of pesticides. Many of the pesticides used are considered toxic to bees, including imidacloprid. Although the bees are not the target of these substances, they are highly vulnerable to contamination. Thereby, the objective of this study was to establish the mean lethal dose (LD50) and the mean lethal concentration (LC50) of imidacloprid for the M. scutellaris. In order to carry out this experiment, bees were collected and the test was performed according to OECD's protocol (1998a, 1998b), developed for A. mellifera. For the determination of LD50 and LC50, data was analyzed through the Probit method. The topical LD50 established in this study was 2.41 ng/ bee for 24 hours and 1.29 ng/bee for 48 hours. The oral LC50 was 2.01 ng i.a./mu L for 24 hours and 0.81 ng i.a./mu L for 48 hours. Thus, it is important to establish management methods which take this higher susceptibility into account to protect native species. Fed Univ Sao Carlos UFSCar, Campus Araras, SP, Brazil Sao Paulo State Univ UNESP, Campus Rio Claro, SP, Brazil Sao Paulo State Univ UNESP, Campus Rio Claro, SP, Brazil FAPESP: 2013/20666-3 : 2012/50197-2

Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-27T11:17:55Z No. of bitstreams: 0Bitstream added on 2014-05-27T14:28:07Z : No. of bitstreams: 1 2-s2.0-0027724633.pdf: 104346 bytes, checksum: 1c59079450dd464a36077fe3e096fceb (MD5) Made available in DSpace on 2014-05-27T11:17:55Z (GMT). No. of bitstreams: 0 Previous issue date: 1993-12-01 Narrow and broad sense heritabilities (h2) of the number of ovarioles in Apis mellifera L. workers were estimated using two distinct data sets, based on groups of half (queens inseminated by several drones) and super-sisters (single drone insemination). The values found are in the usual range for economically important characters (0.25-0.38). Departamento de Biologia IBRC UNESP, Caixa Postal 199, 13506-900 Rio Claro, SP Departamento de Biologia IBRC UNESP, Caixa Postal 199, 13506-900 Rio Claro, SP

Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-27T11:18:05Z No. of bitstreams: 0Bitstream added on 2014-05-27T14:28:24Z : No. of bitstreams: 1 2-s2.0-0029933979.pdf: 509509 bytes, checksum: cf4bf000320dc3934a499e634d63918f (MD5) Made available in DSpace on 2014-05-27T11:18:05Z (GMT). No. of bitstreams: 0 Previous issue date: 1996-06-01 The correspondence between morphometric and isozymic geographic variation patterns of Africanized honey bees in Brazil was analyzed. Morphometric data consisted of mean vectors of 19 wing traits measured in 42 local populations distributed throughout the country. Isozymic data refer to allelic frequencies of malate dehydrogenase (MDH), and were obtained from Lobo and Krieger. The two data sets were analyzed through canonical trend surface, principal components and spatial autocorrelation analyses, and showed north-south dines, demonstrating that Africanized honey bees in southern and southeastern Brazil are more similar to European honey bees than those found in northern and northeastern regions. Also, the morphometric variation is within the limits established by the racial admixture model, considering the expected values of Africanized honey bee fore wing length (WL) in southern and northeastern regions of Brazil, estimated by combining average values of WL in the three main subspecies involved in the Africanization process (Apis mellifera scutellata, A. m. ligustica and A. m. mellifera) with racial admixture coefficients. Departamento de Biologia Geral Instituto de Ciências Biológicas Universidade Federal de Goiás, Caixa Postal 131, 74001-970 Goiânia, GO Departamento de Biologia Instituto de Biociências Universidade Estadual Paulista (UNESP), Caixa Postal 199, 13506-900 Rio Claro, SP Departamento de Biologia Instituto de Biociências Universidade Estadual Paulista (UNESP), Caixa Postal 199, 13506-900 Rio Claro, SP

Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-27T11:18:14Z No. of bitstreams: 0Bitstream added on 2014-05-27T14:28:35Z : No. of bitstreams: 1 2-s2.0-0030872299.pdf: 1066440 bytes, checksum: 66cb3058a13212b39fb9a89ef342a906 (MD5) Made available in DSpace on 2014-05-27T11:18:14Z (GMT). No. of bitstreams: 0 Previous issue date: 1997-06-01 The numbers of sensilla coeloconica and sensilla ampullacea of segment 10 of the antennae of Caucasian and Africanized worker bees and of their hybrids were counted with the aid of a scanning electron microscope. Africanized bees have fewer sensilla than Caucasian bees and the continuous distribution obtained from the F1 values suggests polygenic inheritance for the control of this trait. There are also indications that the hybrid Brazilian Apis mellifera studied here are close to typical African bees (Apis mellifera scutellata) in terms of number of sensilla. Departamento de Biologia Instituto de Biociências UNESP, Caixa Postal 199, 13506-900 Rio Claro, SP Departamento de Biologia Instituto de Biociências UNESP, Caixa Postal 199, 13506-900 Rio Claro, SP

In recent years, studies based on isoenzymatic patterns of geographic variation have revealed that what is usually called the Africanized honey bee does not constitute a single population. Instead, several local populations exist with various degrees of admixture with European honey bees. In this paper, we evaluated new data on morphometric patterns of Africanized honey bees collected at 42 localities in Brazil, using univariate and multivariate (canonical) trend surface and spatial autocorrelation analyses. The clinal patterns of variation found for genetically independent characters (wing size characters and some wing venation angles) are concordant with previous studies of malate dehydrogenase (MDH) allelic frequencies and support the hypothesis that larger honey bees in southern and southeastern Brazil originated by racial admixture in the initial phases of African honey bee colonization. Geographic variation patterns of Africanized honey bee populations reflect a demic diffusion process in which European genes were gradually lost because of the higher fitness of the African gene pool in Neotropical environmental conditions.

Made available in DSpace on 2022-04-28T19:55:56Z (GMT). No. of bitstreams: 0 Previous issue date: 2003-01-01 Two species of plants commonly known as barbatimão occur in Brazil, Stryphnodendron adstringens (Fabaceae) (true barbatimão) and Dimorphandra mollis (Caesalpiniaceae) (false barbatimão). These two species have a similar flowering period and are considered by beekeepers to cause bee mortality during this period. Flowers were collected from both species, dehydrated, ground and incorporated into an experimental diet for bees of two different species, Apis mellifera and Scaptotrigona postica. Both plant species were toxic to A. mellifera, reducing their median survival. D. mollis was toxic to S. postica, and Stryphnodendron adstringens reduced median survival of this bee species even when used at a concentration of 2.5%. In a choice experiment carried out with A. mellifera and the two plant species, the honey bees could choose not to feed on the diets containing the flowers, and feed on sugar and honey instead, but they did not. This shows us that the flowers of S. adstringens were not repellent to the bees. The plants were more toxic to A. mellifera than to Scaptotrigona postica, a result that can be explained by the fact that A. mellifera was introduced into Brazil whereas S. postica is a native stingless bee. © 2003 International Bee Research Association. Centro de Estudos de Insetos Sociais (CEIS) Departamento de Biologia Instituto de Biociências Universidade Estadual Paulista (UNESP), Avenida 24-A, 1515, Rio Claro, SP, CEP-13506-900 Centro de Estudos de Insetos Sociais (CEIS) Departamento de Biologia Instituto de Biociências Universidade Estadual Paulista (UNESP), Avenida 24-A, 1515, Rio Claro, SP, CEP-13506-900

Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T18:58:50Z No. of bitstreams: 1 S0103-50532002000100019.pdf: 111487 bytes, checksum: 9a24aab305292dc59169f44149a5e64a (MD5) Made available in DSpace on 2013-08-22T18:58:50Z (GMT). No. of bitstreams: 1 S0103-50532002000100019.pdf: 111487 bytes, checksum: 9a24aab305292dc59169f44149a5e64a (MD5) Previous issue date: 2002-02-01 Made available in DSpace on 2013-09-30T19:54:55Z (GMT). No. of bitstreams: 2 S0103-50532002000100019.pdf: 111487 bytes, checksum: 9a24aab305292dc59169f44149a5e64a (MD5) S0103-50532002000100019.pdf.txt: 13330 bytes, checksum: 8fe9829036c766859058bf94e0eacad0 (MD5) Previous issue date: 2002-02-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T15:14:16Z No. of bitstreams: 2 S0103-50532002000100019.pdf: 111487 bytes, checksum: 9a24aab305292dc59169f44149a5e64a (MD5) S0103-50532002000100019.pdf.txt: 13330 bytes, checksum: 8fe9829036c766859058bf94e0eacad0 (MD5) Made available in DSpace on 2014-05-20T15:14:16Z (GMT). No. of bitstreams: 2 S0103-50532002000100019.pdf: 111487 bytes, checksum: 9a24aab305292dc59169f44149a5e64a (MD5) S0103-50532002000100019.pdf.txt: 13330 bytes, checksum: 8fe9829036c766859058bf94e0eacad0 (MD5) Previous issue date: 2002-02-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Nesta comunicação estamos reportando as propriedades tóxicas dos extratos metanólicos das flores, pedúnculos, folhas, pecíolo e casca do tronco de Dimorphandra mollis sobre as operárias de Apis mellifera. Astilbina (3-beta-O-rhamnosideo de 5,7,3',4'-- tetraidroxi-2,3-diidroflavonol) isolada dos pedúnculos e flores como o seu principal componente, apresentou atividade inseticida sobre abelhas confinadas. Os resultados obtidos indicam que a astilbina reduz a sobrevivência média das abelhas tratadas. In this communication we have evaluated the toxic properties of methanol extracts from flowers, peduncles, leaves, petioles and stem bark of Dimorphandra mollis to Apis mellifera workers. Astilbin (5,7,3',4'-tetrahydroxy--2,3-dihydroflavonol-3-beta-O-rhamnoside) has been isolated from peduncles and flowers of this plant in large amounts. Astilbin presented insecticidal activity against confined bees. The results suggest that astilbin reduces the average survival of treated bees. Universidade Federal de São Carlos (UFSCar) Departamento de Química Universidade Estadual Paulista Instituto de Biociências Centro de Estudos Insetos Sociais Universidade Estadual Paulista Instituto de Biociências Centro de Estudos Insetos Sociais

A área de computação oferece uma variedade de técnicas e ferramentas com o objetivo de auxiliar na resolução de problemas de diversas áreas de aplicação. O sucesso de um projeto depende muitas vezes da escolha correta dessas ferramentas e das técnicas que serão utilizadas durante as fases do desenvolvimento. Além disso, é importante conhecer previamente as expectativas do usuário e qual seu grau de familiarização com o uso de sistemas informatizados. Esta dissertação de mestrado aborda a utilização de diferentes técnicas e ferramentas de programação na solução de um problema especifico o acasalamento de canários de cor. O trabalho apresenta um levantamento detalhado da evolução dos canários, da sua descoberta até a última mutação registrada envolvendo os genes responsáveis pela cor da plumagem. Para uma melhor compreensão do assunto, é apresentada uma pequena revisão sobre genética e sobre o conhecimento de especialistas da área de canaricultura de cor. Para solucionar o problema, são considerados neste trabalho a utilização de diferentes métodos de desenvolvimento propostos por duas grandes áreas: a Engenharia de Software e a Inteligência Artificial. As técnicas estudadas são implementadas, integrando um Sistema Baseado em Conhecimento e a programação convencional num único sistema. O resultado obtido demonstra que pode-se obter uma solução mais adequada ao problema quando diferentes métodos são combinados. The computing area offers a variety of techniques and tools with the objective of helping the resolution of problems from several applications areas. The success of a design depends many times on the correct choice of these tools and techniques which will be used during the phases of development. Moreover, it is important to Icnow previously the expectation of the user and how is his degree of familiarization with the use of information systems. This MSc dissertation approaches the use of different techniques and programming tools in the solution of a specific problem - the colored canaries breeding. The work presents a detailed survey of the evolution of the canaries, since its discovery until the last registered mutation involving the responsible genes for the colour of the plumage. For better understanding, the dissertation presents a short revision about genetics and specialists icnowledge about coloured canaries. To solve the problem, this work considers the use of different methods of development from two wide areas: Software Engineering and Artificial Intelligence. The studied techniques are implemented, integrating Knowledge Based System and conventional programming in the same system. The final results demonstrate that a better solution for a given problem can be achieved by combining different methods.

O melhoramento genético de abelhas Apis mellifera é uma ferramenta essencial e de caráter obrigatório para o sucesso e desenvolvimento do setor apícola. O nosso objetivo principal foi estruturar, estabelecer e fixar um plano de seleção e melhoramento genético com o objetivo de incrementar a produção de própolis por colmeia. Iniciamos este trabalho através da avaliação das características de seleção de comportamento higiênico, taxa de infestação de Varroa, incidência de Nosema em um grupo inicial de 50 colmeias. Neste grupo 40,4% das colmeias apresentaram um comportamento higiênico acima de 90% com valores de 71 ± 28,9 ± 4,22 (média ± DP ± EP); taxa de infestação de Varroa 7,7 ± 4,0 ± 0,57 (média ± DP ± EP) e uma média de incidência de Nosema < 300.000 esporos/abelha. Após estas avaliações, selecionamos três colmeias com os seguintes parâmetros: >90% de comportamento higiênico, 95% de comportamento higiênico, 90% hygienic behavior, 95% hygienic behavior

O presente trabalho teve por objetivo estudar a atividade antifúngica e antibacteriana de diferentes tipos de própolis, tanto de abelhas africanizadas (verde) quanto meliponíneos. A concentração inibitória mínima, sendo a menor concentração de um agente inibidor em que não se observa o crescimento macroscópico do microorganismo, foi determinada para todas as amostras de própolis. Para análise do perfil químico das amostras foi utilizada como metodologia (CLAE), visando determinar as classes de substâncias presentes nas amostras de própolis. Em todos os microorganismos testados as própolis das abelhas Tetragonisca angustula, Plebeia droryana, Scaptotrigona bipunctata, Friesiomelitta varia e Nannotrigona testaceicornis não apresentaram atividade antifúngica nem antibacteriana. Nenhuma das amostras de própolis testadas apresentou atividade antifúngica frente ao Aspergillus nidulans. A linhagem do fungo Trichophyton rubrum mostrou-se sensível a alguns tipos de própolis sendo que a própolis verde foi mais eficaz. Alguns tipos de própolis apresentaram atividade antibacteriana, indicando a importância dessas resinas na terapêutica humana e veterinária. Our objective was to examine the antifungal and antibacterial activities of several types of propolis, including Africanized honey bee green propolis and propolis produced by meliponini bees. The minimal inhibitory concentration, considered to be the lowest concentration of an inhibitory agent in which there is no macroscopic growth of the microorganism, was determined for all the propolis samples. CLAE methodology was used for the analysis of the chemical profiles of the samples, in order to determine the classes of substances in the propolis samples. Propolis from the bees Tetragonisca angustula, Plebeia droryana, Scaptotrigona bipunctata, Friesiomelita varia and Nannotrigona testaceicornis presented no antifungal or antibacterial activity. None of the propolis samples presented antifungal activity against Aspergillus nidulans. The fungus Trichophyton rubrum was sensitive to several types of propolis, green propolis being the most efficient against this species. Various types of propolis also presented antibacterial activity, demonstrating the potential importance of these resins for human and veterinary medicine.