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652 results on '"Ogawa, Takuya"'

104. Nasal obstruction during the growth period modulates the Wnt/β‐catenin pathway and brain‐derived neurotrophic factor production in association with tyrosine kinase receptor B mRNA reduction in mouse hippocampus.

Author

Ishidori, Hideyuki, Okihara, Hidemasa, Ogawa, Takuya, Abe, Yasunori, Kato, Chiho, Aung, Phyo Thura, Fujita, Akiyo, Kokai, Satoshi, and Ono, Takashi

Subjects
BRAIN-derived neurotrophic factor, PROTEIN-tyrosine kinases, FACTORS of production, OXYGEN in the blood, HIPPOCAMPUS (Brain)
Abstract

There is accumulating evidence that nasal obstruction induces high‐level brain dysfunction, including memory and learning deficits. We previously demonstrated that unilateral nasal obstruction (UNO) during the growth period increases the expression of brain‐derived neurotrophic factor (BDNF). The expression of BDNF is regulated by the Wnt/β‐Catenin pathway, which is linked to neuronal differentiation, proliferation, and maintenance. However, little is known about whether Wnt3a protein expression could be an index for modulations analyses in the Wnt/β‐Catenin pathway caused by UNO during the growth period. This study aimed to investigate the effects of UNO during the growth period on the Wnt/β‐Catenin pathway in the hippocampus using combined behavioural, biochemical, and histological approaches. Male BALB/C mice were randomly divided into the control (CONT; n = 6) and experimental (UNO; n = 6) groups. Blood oxygen saturation (SpO2) levels were measured, and a passive avoidance test was performed in mice aged 15 weeks. Brain tissues were subjected to immunohistochemistry, real‐time reverse transcription‐polymerase chain reaction, and western blot analysis. Compared with control mice, UNO mice had lower SpO2 levels and exhibited memory/learning impairments during behavioural testing. Moreover, Wnt3a protein, BDNF mRNA, and tyrosine kinase receptor B (TrkB) mRNA expression levels were significantly lower in the hippocampus in the UNO group than in the CONT group. Our findings suggested that UNO during the growth period appeared to modulate the hippocampal Wnt/β‐catenin pathway and BDNF production in association with TrkB mRNA reduction, thereby resulting in memory and learning impairments. [ABSTRACT FROM AUTHOR]

Published
2022
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105. Additional file 1: of Functional dysbiosis within dental plaque microbiota in cleft lip and palate patients

Author

Funahashi, Kenta, Shiba, Takahiko, Watanabe, Takayasu, Muramoto, Keiko, Takeuchi, Yasuo, Ogawa, Takuya, Izumi, Yuichi, Sekizaki, Tsutomu, Nakagawa, Ichiro, and Moriyama, Keiji

Abstract

Figure S1. Number of OTUs, Shannonâ s diversity index, and rarefaction curves. Figure S2. PCoA plots with information of appliances. Figure S3. Scatter graphs of mRNA abundances for pairs of interacting core taxa. Figure S4. PCoA plots for the functional profiles assigned using BLASTX. Table S1. Summary of Illumina MiSeq reads and the derived data. Table S2. Rank distribution of species assigned for the rc-rRNA OTUs (relative abundance per participant). Table S3. Rank distribution of the level-1 SEED subsystem functions assigned for the mRNA reads (relative abundance per participant). Table S4. Rank distribution of the level-2 KEGG functions assigned for the mRNA reads (relative abundance per participant). Table S5. Rank distribution of the top 50 species assigned for the mRNA reads (relative abundance per participant). Table S6. Rank distribution of VTiF (mean RPKM values among the participants and the mRNA-to-rRNA ratio). Table S7. Rank distribution of the top 50 VFDB functions assigned for the mRNA reads (relative abundance per participant). Table S8. Rank distribution of the top 50 MvirDB functions assigned for the mRNA reads (relative abundance per participant). (PDF 454 kb)

Published
2019
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106. Development of a regulatable low-temperature protein expression system using the psychrotrophic bacterium, Shewanella livingstonensis Ac10, as the host

Author

40756318, 70243087, Kawai, Soichiro, Kawamoto, Jun, Ogawa, Takuya, Kurihara, Tatsuo, 40756318, 70243087, Kawai, Soichiro, Kawamoto, Jun, Ogawa, Takuya, and Kurihara, Tatsuo

Abstract

A low-temperature protein expression system is useful for the production of thermolabile proteins. We previously developed a system that enables constitutive protein production at low temperatures, using the psychrotrophic bacterium Shewanella livingstonensis Ac10 as the host. To increase the utility of this system, in the present study, we introduced a repressible promoter of the trp operon of this bacterium into the system. When ß-lactamase was produced under the control of this promoter at 18°C and 4°C, the yields were 75 and 33 mg/L-culture, respectively, in the absence of L-Trp, and the yields were decreased by 72% and 77%, respectively, in the presence of L-Trp. We also found that 3-indoleacrylic acid, a competitive inhibitor of the Escherichia coli trp repressor, increased the expression of the reporter gene. This repressible gene expression system would be useful for regulatable recombinant protein production at low temperatures.

Published
2019

124. Easy and environmentally friendly synthesis method for T8H (HSiO3/2)8.

Author

Tsukada, Satoru, Ogawa, Takuya, Susami, Yuma, Yamamoto, Kazuki, and Gunji, Takahiro

Subjects
*HYDROGEN chloride, *TRICHLOROSILANE, *AQUEOUS solutions, *METHANOL, *GASES
Abstract

Environmentally friendly methods for the synthesis of T8H were developed. A combination of triethoxysilane (TES), concentrated HCl aqueous solution, and FeCl3 produced T8H with almost the same yield as the previously known methods. We also found that HNO3 could be used instead of HCl, resulting in a similar yield. No toxic gases were generated during the reaction with HNO3. Additionally, the synthesis of T8H based on TES did not generate dangerous substances such as hydrogen chloride and methanol, unlike when trichlorosilane or trimethoxysilane are used. Therefore, our new method for the synthesis of T8H is considered to be safer, although the yield is the same as the conventional methods. [ABSTRACT FROM AUTHOR]

Published
2021
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125. Easy and environmentally friendly synthesis method for T8H (HSiO3/2)8.

Author

Tsukada, Satoru, Ogawa, Takuya, Susami, Yuma, Yamamoto, Kazuki, and Gunji, Takahiro

Subjects
HYDROGEN chloride, TRICHLOROSILANE, AQUEOUS solutions, METHANOL, GASES
Abstract

Environmentally friendly methods for the synthesis of T8H were developed. A combination of triethoxysilane (TES), concentrated HCl aqueous solution, and FeCl3 produced T8H with almost the same yield as the previously known methods. We also found that HNO3 could be used instead of HCl, resulting in a similar yield. No toxic gases were generated during the reaction with HNO3. Additionally, the synthesis of T8H based on TES did not generate dangerous substances such as hydrogen chloride and methanol, unlike when trichlorosilane or trimethoxysilane are used. Therefore, our new method for the synthesis of T8H is considered to be safer, although the yield is the same as the conventional methods. [ABSTRACT FROM AUTHOR]

Published
2021
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127. Long-Term Management and Maxillofacial Growth in a Klippel-Trenaunay Syndrome Patient.

Author

Ogawa, Takuya, Cheng, Eric Shih-Wei, Muramoto, Keiko, and Moriyama, Keiji

Subjects
FACIAL bone growth, PATIENT aftercare, MANDIBLE, MAXILLA, DENTAL occlusion, ORTHODONTIC appliances, DISEASE management, KLIPPEL-Trenaunay-Weber Syndrome, CHILDREN
Abstract

Klippel-Trenaunay syndrome (KTS) is a congenital disorder associated with capillary, venous, lymphatic vascular malformations, and unilateral hypertrophy of the soft tissue and bone. We report a case of a 5-year-old girl with KTS who was followed up until age 17. The asymmetry of her maxillary dentition became remarkable with growth, although no significant left-right difference in either the maxilla or mandible was recognized. Acceptable occlusion was achieved without fixed orthodontic appliances; however, it was necessary to develop treatment plans in accordance with the general symptoms of the disease. [ABSTRACT FROM AUTHOR]

Published
2020
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131. Enhanced vulnerability to oxidative stress and induction of inflammatory gene expression in 3‐phosphoglycerate dehydrogenase‐deficient fibroblasts

Author

Hamano, Momoko, primary, Haraguchi, Yurina, additional, Sayano, Tomoko, additional, Zyao, Chong, additional, Arimoto, Yashiho, additional, Kawano, Yui, additional, Moriyasu, Kazuki, additional, Udono, Miyako, additional, Katakura, Yoshinori, additional, Ogawa, Takuya, additional, Kato, Hisanori, additional, and Furuya, Shigeki, additional

Published
2018
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133. Nasal obstruction during adolescence induces memory/learning impairments associated with BDNF/TrkB signaling pathway hypofunction and high corticosterone levels

Author

Ogawa, Takuya, primary, Okihara, Hidemasa, additional, Kokai, Satoshi, additional, Abe, Yasunori, additional, Karin Harumi, Uchima Koecklin, additional, Makiguchi, Mio, additional, Kato, Chiho, additional, Yabushita, Tadachika, additional, Michikawa, Makoto, additional, and Ono, Takashi, additional

Published
2018
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137. Identification of OPN3as associated with non-syndromic oligodontia in a Japanese population

Author

Inagaki, Yumi, Ogawa, Takuya, Tabata, Makoto J., Nagata, Yuki, Watanabe, Ryo, Kawamoto, Tatsuo, Moriyama, Keiji, and Tanaka, Toshihiro

Abstract

Tooth agenesis is one of the most frequent congenital abnormalities found in the maxillofacial region. Oligodontia, a severe form of tooth agenesis, occurs as an isolated anomaly or as a syndromic feature. We performed whole exome sequencing analyses to identify causative mutation in a Japanese family with three affected individuals with non-syndromic oligodontia. After variant filtering procedures and validation by Sanger sequencing, we identified one missense mutation (c.668 C > T, p.Gly223Asp) in OPN3at 1q43, encoding a photosensitive G-protein-coupled receptor (GPCR) expressed in various tissues including brain, liver, and adipose. This mutation was predicted to be pathogenic in silico and was not found in the public databases. We further examined 48 genetically unrelated cases by targeted sequencing of the OPN3 gene region and found one additional missense variant in this gene (c.768 C > T, p.Met256Ile) that was also predicted to be pathogenic. Localization of OPN3 protein by immunohistochemical analysis using mouse embryo revealed its specific expression in the tooth gems from bud to bell stages and their surrounding tissues. These results indicated that OPN3was involved in non-syndromic oligodontia, which has made an anchoring point for clinical application including DNA diagnostics.

Published
2021
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146. Lysophosphatidic acid acyltransferase from the thermophilic bacterium Thermus thermophilusHB8 displays substrate promiscuity

Author

Ogawa, Takuya, Suwanawat, Nittikarn, Toyotake, Yosuke, Watanabe, Bunta, Kawamoto, Jun, and Kurihara, Tatsuo

Abstract

ABSTRACTLysophosphatidic acid acyltransferase is a phospholipid biosynthetic enzyme that introduces a fatty acyl group into the sn-2 position of phospholipids. Its substrate selectivity is physiologically important in defining the physicochemical properties of lipid membranes and modulating membrane protein function. However, it remains unclear how these enzymes recognize various fatty acids. Successful purification of bacterial lysophosphatidic acid acyltransferases (PlsCs) was recently reported and has paved a path for the detailed analysis of their reaction mechanisms. Here, we purified and characterized PlsC from the thermophilic bacterium Thermus thermophilusHB8. This integral membrane protein remained active even after solubilization and purification and showed reactivity toward saturated, unsaturated, and methyl-branched fatty acids, although branched-chain acyl groups are the major constituent of phospholipids of this bacterium. Multiple sequence alignment revealed the N-terminal end of the enzyme to be shorter than that of PlsCs with defined substrate selectivity, suggesting that the shortened N-terminus confers substrate promiscuity.AbbreviationsACP: acyl carrier protein; CAPS: N-cyclohexyl-3-aminopropanesulfonic acid; CoA: coenzyme A; CYMAL-6: 6-cyclohexyl-1-hexyl-β-D-maltoside; DDM: n-dodecyl-β-D-maltoside; DTNB: 5,5´-dithiobis(2-nitrobenzoic acid); EPA: eicosapentaenoic acid; G3P: glycerol 3-phosphate; HEPES: N-2-hydroxyethylpiperazine-N´-2-ethanesulfonic acid; LPA: lysophosphatidic acid; MS: mass spectrometry; PA: phosphatidic acid.

Published
2020
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147. 22‐5: Late‐News Paper:Silicone‐based Low‐k Material for Display

Author

Park, Yungjin, Swatowski, Brandon, Ogawa, Takuya, Jang, EunKyung, Fu, Peng-Fei, and Chung, Myunhwa

Abstract

The encapsulation and insulation layers, which are typically located between a data electrode and a pixel electrode, should have low parasitic capacitance and enough electrical insulation to minimize signal distortion and crosstalk. Low dielectric constant (Dk) material reduces the parasitic capacitance and improve the insulation performance. New OLED flexible displays need organic‐based passivation and insulation materials for flexibility, light weight and productivity. Epoxy and acryl‐based materials were widely used in display industry for this purpose, but both have relatively higher Dk greater than 3.0, which limits to design new flexible displays and touch screen panels. DOW has invented a low Dk optical material based on silicone technology. A Dk value of 2.5 was achieved with a low viscosity of 12–18 mPa.s at 25°C for jetting without solvent. The newly developed product can be cured at 1,000 mJ/cm2energy density at 395 nm with a light emitting diode (LED) ultraviolet (UV) lamp. One of the target applications is a dielectric layer for touch screen panels on organic light emitting diode (OLED) displays, but it is not limited to this. This material enables display manufacturers to develop and design ultrathin and innovative touch screen panels; and the novel and patented technology can be applied to various applications that require low dielectric constant.

Published
2020
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148. Study on the Conduction Mechanism of Organic Light-Emitting Diode Using One-Dimensional Discontinuous Model

Author

OGAWA, Takuya, CHO, Don-Chan, KANEKO, Kazue, MORI, Tatsuo, and MIZUTANI, Teruyoshi

Subjects
organic light emitting diode (OLED), carrier injection, field distortion, Fowler-Nordheim emission, hopping conduction
Abstract

We proposed the conduction mechanism of organic light-emitting diode (OLED) using a one-dimensional discontinuous model. We assumed that each emitting molecule corresponds to a hopping site according to the actual charge transfer between adjacent molecules. Both carrier mobility of Alq3 and barrier heights for each carrier were derived from experimental data. We calculate transient behavior of carrier, field, and exciton distribution. Both carrier injections assumed the Schottky injection. In the previous results, when we assumed that calculated current density fit the experimental one in the current density field curve, calculated light-emission intensity did not fit the experimental one in the light-emission field curve. Furthermore, the slope of the calculated light emission-field curve is too small to fit the experimental one. In the previous study, hopping distance was assumed to be 1 nm. In this study, it is assumed to be 1.7 nm. We consider that field dependence of electron injection is too weak to explain only the Schottky emission. When the electron injection is assumed to be both Schottky emission and Fowler-Nordheim emission calculated light-emission field as well as the current-density field curves were fit to the curve of each experimental characteristics.

Published
2002

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