109 results on '"Noel Ellis"'
Search Results
102. Vicilin genes ofPisum
- Author
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Claire Domoney, Wendy Cleary, T. H. Noel Ellis, D. Roy Davies, and Judy Castleton
- Subjects
Chromosome 7 (human) ,Genetics ,chemistry.chemical_classification ,biology ,biology.organism_classification ,Human genetics ,Pisum ,chemistry ,Gene mapping ,Complementary DNA ,Vicilin ,Storage protein ,Molecular Biology ,Gene - Abstract
We describe four genomic clones of pea 7S storage protein gene, one of which corresponds to convicilin, and the others to vicilin. Hybridization studies exploiting these clones, and previously identified cDNA clones, have enabled us to define six different loci. Three of these loci have been mapped to positions on chromosome 7.
- Published
- 1986
103. UNIFOLIATA regulates leaf and flower morphogenesis in pea
- Author
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Noel Ellis, Lynda Turner, Mike Ambrose, Julie M.I. Hofer, Peter Matthews, Roger P. Hellens, and Anthony J. Michael
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Molecular Sequence Data ,Mutant ,Genes, Plant ,Polymerase Chain Reaction ,General Biochemistry, Genetics and Molecular Biology ,Gene Expression Regulation, Plant ,Arabidopsis ,Amino Acid Sequence ,Leafy ,Gene ,Alleles ,In Situ Hybridization ,DNA Primers ,Plant Proteins ,Regulator gene ,Genetics ,Base Sequence ,Sequence Homology, Amino Acid ,Agricultural and Biological Sciences(all) ,biology ,Biochemistry, Genetics and Molecular Biology(all) ,fungi ,Antirrhinum ,Peas ,Gene Expression Regulation, Developmental ,food and beverages ,Meristem ,biology.organism_classification ,Plant Leaves ,body regions ,Phenotype ,Mutation ,General Agricultural and Biological Sciences ,human activities ,Flower morphogenesis - Abstract
Background: The vegetative phenotype of the pea mutant unifoliata (uni) is a simplification of the wild-type compound leaf to a single leaflet. Mutant uni plants are also self-sterile and the flowers resemble known floral meristem and organ identity mutants. In Antirrhinum and Arabidopsis , mutations in the floral meristem identity gene FLORICAULA/LEAFY (FLO/LFY) affect flower development alone, whereas the tobacco FLO/LFY homologue, NFL , is expressed in vegetative tissues, suggesting that NFL specifies determinacy in the progenitor cells for both flowers and leaves. In this paper, we characterised the pea homologue of FLO/LFY . Results: The pea cDNA homologue of FLO/LFY, PEAFLO , mapped to the uni locus in recombinant-inbred mapping populations and markers based on PEAFLO cosegregated with uni in segregating sibling populations. The characterisation of two spontaneous uni mutant alleles, one containing a deletion and the other a point mutation in the PEAFLO coding sequences, predicted that PEAFLO corresponds to UNI and that the mutant vegetative phenotype was conferred by the defective PEAFLO gene. Conclusions: The uni mutant demonstrates that there are shared regulatory processes in the morphogenesis of leaves and flowers and that floral meristem identity genes have an extended role in plant development. Pleiotropic regulatory genes such as UNI support the hypothesis that leaves and flowers derive from a common ancestral sporophyll-like structure. The regulation of indeterminacy during leaf and flower morphogenesis by UNI may reflect a primitive function for the gene in the pre-angiosperm era.
104. Book Reviews
- Author
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Noel Ellis
- Subjects
Genetics ,Genetics (clinical) - Published
- 1989
105. Potential and limits of exploitation of crop wild relatives for pea, lentil, and chickpea improvement
- Author
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Clarice J. Coyne, Shiv Kumar, Eric J.B. vonWettberg, Edward Marques, Jens D. Berger, Robert J. Redden, T.H. Noel Ellis, Jan Brus, Lenka Zablatzká, and Petr Smýkal
- Subjects
chickpea ,climate change ,crop wild relatives ,genetic diversity ,introgression ,lentil ,Plant culture ,SB1-1110 - Abstract
Abstract Legumes represent the second most important family of crop plants after grasses, accounting for approximately 27% of the world's crop production. Past domestication processes resulted in a high degree of relatedness between modern varieties of crops, leading to a narrower genetic base of cultivated germplasm prone to pests and diseases. Crop wild relatives (CWRs) harbor genetic diversity tested by natural selection in a range of environments. To fully understand and exploit local adaptation in CWR, studies in geographical centers of origin combining ecology, physiology, and genetics are needed. With the advent of modern genomics and computation, combined with systematic phenotyping, it is feasible to revisit wild accessions and landraces and prioritize their use for breeding, providing sources of disease resistances; tolerances of drought, heat, frost, and salinity abiotic stresses; nutrient densities across major and minor elements; and food quality traits. Establishment of hybrid populations with CWRs gives breeders a considerable benefit of a prebreeding tool for identifying and harnessing wild alleles and provides extremely valuable long‐term resources. There is a need of further collecting and both ex situ and in situ conservation of CWR diversity of these taxa in the face of habitat loss and degradation and climate change. In this review, we focus on three legume crops domesticated in the Fertile Crescent, pea, chickpea, and lentil, and summarize the current state and potential of their respective CWR taxa for crop improvement.
- Published
- 2020
- Full Text
- View/download PDF
106. Genome-Wide Association Mapping for Agronomic and Seed Quality Traits of Field Pea (Pisum sativum L.)
- Author
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Krishna Kishore Gali, Alison Sackville, Endale G. Tafesse, V.B. Reddy Lachagari, Kevin McPhee, Mick Hybl, Alexander Mikić, Petr Smýkal, Rebecca McGee, Judith Burstin, Claire Domoney, T.H. Noel Ellis, Bunyamin Tar'an, and Thomas D. Warkentin
- Subjects
field pea ,genetic diversity ,genome-wide association study ,genotyping-by-sequencing ,single nucleotide polymorphisms ,Plant culture ,SB1-1110 - Abstract
Genome-wide association study (GWAS) was conducted to identify loci associated with agronomic (days to flowering, days to maturity, plant height, seed yield and seed weight), seed morphology (shape and dimpling), and seed quality (protein, starch, and fiber concentrations) traits of field pea (Pisum sativum L.). A collection of 135 pea accessions from 23 different breeding programs in Africa (Ethiopia), Asia (India), Australia, Europe (Belarus, Czech Republic, Denmark, France, Lithuania, Netherlands, Russia, Sweden, Ukraine and United Kingdom), and North America (Canada and USA), was used for the GWAS. The accessions were genotyped using genotyping-by-sequencing (GBS). After filtering for a minimum read depth of five, and minor allele frequency of 0.05, 16,877 high quality SNPs were selected to determine marker-trait associations (MTA). The LD decay (LD1/2max,90) across the chromosomes varied from 20 to 80 kb. Population structure analysis grouped the accessions into nine subpopulations. The accessions were evaluated in multi-year, multi-location trials in Olomouc (Czech Republic), Fargo, North Dakota (USA), and Rosthern and Sutherland, Saskatchewan (Canada) from 2013 to 2017. Each trait was phenotyped in at least five location-years. MTAs that were consistent across multiple trials were identified. Chr5LG3_566189651 and Chr5LG3_572899434 for plant height, Chr2LG1_409403647 for lodging resistance, Chr1LG6_57305683 and Chr1LG6_366513463 for grain yield, Chr1LG6_176606388, Chr2LG1_457185, Chr3LG5_234519042 and Chr7LG7_8229439 for seed starch concentration, and Chr3LG5_194530376 for seed protein concentration were identified from different locations and years. This research identified SNP markers associated with important traits in pea that have potential for marker-assisted selection towards rapid cultivar improvement.
- Published
- 2019
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107. Diversity of Pod Shape in Pisum
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Thomas Henry Noel Ellis, Julie M. I. Hofer, Eleni Vikeli, Michael J. Ambrose, Paola Higuera-Poveda, Luzie U. Wingen, and Noam Chayut
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pea ,germplasm ,Pisum sativum ,Pisum fulvum ,cv Afghanistan ,pod ,Biology (General) ,QH301-705.5 - Abstract
The seed-containing pod is the defining structure of plants in the legume family, yet pods exhibit a wide range of morphological variation. Within a species pod characters are likely to be correlated with reproductive strategy, and within cultivated forms will correspond to aspects of yield determination and/or end use. Here variation in pod size, described as pod length: pod width ratio, has been analyzed in pea germplasm represented by 597 accessions. This pod size variation is discussed with respect to population structure and to known classical pod morphology mutants. Variability of the pod length: width ratio can be explained by allelic variation at two genetic loci that may correspond to organ-specific negative regulators of growth.
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- 2021
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108. Nodule-Expressed Cyp15a Cysteine Protease Genes Map to Syntenic Genome Regions in Pisum and Medicago spp.
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Jason L. Vincent, Maggie R. Knox, T. H. Noel Ellis, Péter Kaló, György B. Kiss, and Nicholas J. Brewin
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transformation ,Microbiology ,QR1-502 ,Botany ,QK1-989 - Abstract
PsCyp15a is a gene that encodes a vacuolar cysteine protease expressed in wilt-induced shoots of Pisum sativum (pea) and in root nodules. To further the understanding of nodular PsCyp15a expression, a region 5′ to the coding sequence of the gene was cloned. Varying lengths of 5′ untranslated sequence were fused with the uidA coding region and introduced from Agrobacterium rhizogenes into “hairy roots” of Vicia hirsuta. In this transgenic root nodulation assay, a promoter sequence of 900 bp was sufficient to give an expression pattern indistinguishable from that obtained in pea nodules by in situ hybridization. An orthologue of PsCyp15a was cloned from nodule mRNA of Medicago sativa and a corresponding gene identified in M. truncatula was also shown to express strongly in nodules. With molecular mapping techniques, it was demonstrated that these genes map to a syntenic genome location in pea and Medicago spp., but the map positions of the Cyp15a genes cannot be correlated with existing nodulation mutants.
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- 2000
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109. Identification of Mendel's white flower character.
- Author
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Roger P Hellens, Carol Moreau, Kui Lin-Wang, Kathy E Schwinn, Susan J Thomson, Mark W E J Fiers, Tonya J Frew, Sarah R Murray, Julie M I Hofer, Jeanne M E Jacobs, Kevin M Davies, Andrew C Allan, Abdelhafid Bendahmane, Clarice J Coyne, Gail M Timmerman-Vaughan, and T H Noel Ellis
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Medicine ,Science - Abstract
The genetic regulation of flower color has been widely studied, notably as a character used by Mendel and his predecessors in the study of inheritance in pea.We used the genome sequence of model legumes, together with their known synteny to the pea genome to identify candidate genes for the A and A2 loci in pea. We then used a combination of genetic mapping, fast neutron mutant analysis, allelic diversity, transcript quantification and transient expression complementation studies to confirm the identity of the candidates.We have identified the pea genes A and A2. A is the factor determining anthocyanin pigmentation in pea that was used by Gregor Mendel 150 years ago in his study of inheritance. The A gene encodes a bHLH transcription factor. The white flowered mutant allele most likely used by Mendel is a simple G to A transition in a splice donor site that leads to a mis-spliced mRNA with a premature stop codon, and we have identified a second rare mutant allele. The A2 gene encodes a WD40 protein that is part of an evolutionarily conserved regulatory complex.
- Published
- 2010
- Full Text
- View/download PDF
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