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103 results on '"Nobuhiko Okada"'

101. Production and characterization of monoclonal antibodies to Tyzzer's organism 'Bacillus piliformis'

Author

Kosaku Fujiwara, Wataru Toriumi, Nobuhiko Okada, and Seiji Kawamura

Subjects
Mice, Inbred BALB C, medicine.diagnostic_test, medicine.drug_class, Cell, Antibodies, Monoclonal, Bacillus, General Medicine, Biology, Monoclonal antibody, Immunofluorescence, Molecular biology, Antibodies, Bacterial, Bacterial cell structure, Clone Cells, Blot, Mice, medicine.anatomical_structure, Antigen, Splenocyte, medicine, Animals, Bacterial antigen
Abstract

Two cell clones producing monoclonal antibodies (MAb) to the RT strain of Tyzzer's organism ('Bacillus piliformis'), of rat origin, were established by fusion of immunized BALB/c mouse splenocytes and NSl1 cells. Western blotting and immunofluorescence (IF) revealed that one of the MAb recognized an bacterial antigen having a molecular weight (M.W.) of 54, 000 and combined with the bacterial flagella, while the other recognized another antigen with M.W. of 73, 000 and combined with the bacterial cell surface but not with flagella. The former reacted also with antigens of mouse derived bacterial strain MSK of B. piliformis the latter did not. Both MAb were protective for the challenge infection with B. piliformis, reducing the number of necrotic foci in the liver of infected mice as well as that of cytopathic plaques on mouse hepatocyte culture.

Published
1986

102. Liver lesions and serum antibodies in mice surviving Tyzzer's disease

Author

Aisuke Nii, Akinori Yasuda, Kosaku Fujiwara, Hiroyuki Nakayama, Nobuhiko Okada, and Satoshi Inoue

Subjects
Pathology, medicine.medical_specialty, Mice, Inbred ICR, biology, Inoculation, Liver Diseases, chemistry.chemical_element, Bacillus, General Medicine, Calcium, medicine.disease, Antibodies, Bacterial, Serum antibody, Tyzzer's disease, Mice, chemistry, Giant cell, Granuloma, Giant Cell, medicine, biology.protein, Animals, Female, Bacterial antigen, Antibody, Intracellular
Abstract

In the liver of mice surviving for 2 weeks or more after intravenous inoculation of Tyzzer's organisms, MSK strain, were observed giant cell granulomas with intracellular deposition of mucopolysaccharide and calcium, while bacterial antigen was not detectable. Serum antibody was positive in all cases already at 2 weeks postinoculation and high-titered antibody remained at 9 weeks of infection.

Published
1986

103. Extracellular vesicles produced by Bifidobacterium longum export mucin-binding proteins.

Author

Keita Nishiyam, Takashi Takaki, Makoto Sugiyama, Itsuko Fukuda, Maho Aiso, Takao Mukai, Toshitaka Odamaki, Jin-zhong Xiao, Ro Osawa, and Nobuhiko Okada

Subjects
*EXTRACELLULAR vesicles, *BIFIDOBACTERIUM longum, *PHOSPHOGLYCERATE kinase, *PROTEINS, *GASTROINTESTINAL system
Abstract

Extracellular proteins are important factors in host-microbe interactions; however, the specific factors that enable bifidobacterial adhesion and survival in the gastrointestinal (GI) tract are not fully characterized. Here, we discovered that Bifidobacterium longum NCC2705 cultured in bacterium-free supernatants of human fecal fermentation broth released a myriad of particles into the extracellular environment. The aim of this study was to characterize the physiological properties of these extracellular particles. The particles, approximately 50 to 80 nm in diameter, had high protein and double-stranded DNA contents, suggesting that they were extracellular vesicles (EVs). A proteomic analysis showed that the EVs primarily consisted of cytoplasmic proteins with crucial functions in essential cellular processes. We identified several mucin-binding proteins by performing a biomolecular interaction analysis of phosphoketolase, GroEL, elongation factor Tu (EF-Tu), phosphoglycerate kinase, transaldolase (Tal), and heat shock protein 20 (Hsp20). The recombinant GroEL and Tal proteins showed high binding affinities to mucin. Furthermore, the immobilization of these proteins on microbeads affected the permanence of the microbeads in the murine GI tract. These results suggest that bifidobacterial exposure conditions that mimic the intestine stimulate B. longum EV production. The resulting EVs exported several cytoplasmic proteins that may have promoted B. longum adhesion. This study improved our understanding of the Bifidobacterium colonization strategy in the intestinal microbiome. [ABSTRACT FROM AUTHOR]

Published
2020
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