367 results on '"Moscone D"'
Search Results
102. Aflatoxin M1 analysis in milk: reference methods for the validation of electrochemical immunosensors
- Author
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Marconi, Emanuele, Panfili, Gianfranco, Messia, Maria Cristina, Candigliota, T, Moscone, D, Micheli, L, and Palleschi, G.
- Published
- 2003
103. Cyanobacteria attack rocks (CATS):Control and preventive strategies to avoid damage caused by cyanobacteria and associated microorganisms in Roman hypogean monuments
- Author
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Albertano, P., Moscone, D., Palleschi, G., Hermosin, B., Saiz-Jimenez, C., Sanchez-Moral, S., Hernandez-Marine, M., Urzi, C., Groth, I., Schroeckh, V., Saarela, Maria, Mattila-Sandholm, Tiina, Gallon, J. R., Graziottin, F., Bisconti, F., Giuliani, R., and Saiz-Jimenez, C.
- Abstract
The CATS project focuses on the control, prevention and monitoring of cyanobacteria-dominated biofilms that cause damage to rock surfaces in Roman hypogea. It develops and integrates physical and biotechnological methods intended to limit the growth of microorganisms on valuable archaeological surfaces, and applies analytical methods to monitor the presence and the extent of the microbial damage. As in other hypogea, the development of biofilms is favoured by the limited air circulation, the even temperature throughout the year, and the high level of humidity. Biofilms composed of sciaphilous chroococcal and filamentous cyanobacteria associated with other microorganisms develop thanks to the light gradients that occur in the proximity of entrances and artificial lamps. Terrestrial cyanobacteria and associated microorganisms are the first colonisers of exposed lithic faces and their extensive development is supported by the mineral composition of the substrata and facilitated by the porous nature of the, mostly calcareous, surfaces.
- Published
- 2003
104. Biosensori elettrochimici per applicazioni analitiche nel settore lattiero-caseario
- Author
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Palleschi, G, Compagnone, Dario, Moscone, D, Isoldi, G., Pallini, M., Volpe, G., Esti, M, and Marconi, E.
- Published
- 2000
105. Amperometric biosensors for food quality control. Determination of biogenic amines, lactulose and glycerol
- Author
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Compagnone, Dario, Moscone, D, and Palleschi, G.
- Published
- 1999
106. Development and application of amperometric biosensors in food analysis
- Author
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Compagnone, Dario, Moscone, D, and Palleschi, G.
- Published
- 1998
107. Electrochemical biosensors for food analysis
- Author
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Palleschi, G, Baldino, C, Compagnone, D, Esti, M, Messia, Maria Cristina, and Moscone, D.
- Published
- 1997
108. Biosensori elettrochimici in biomedicina
- Author
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Palleschi, G, Moscone, D, and Compagnone, Dario
- Published
- 1997
109. Electrochemical biosensors: potential and application in the food industry
- Author
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Palleschi, G, Compagnone, Dario, and Moscone, D.
- Published
- 1997
110. Biosensori elettrochimici per la valutazione della qualità di pesche e kiwi
- Author
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Esti, M, Messia, Maria Cristina, Bertocchi, P, Moscone, D, and Palleschi, G.
- Published
- 1996
111. POTENTIOMETRIC MICROSENSORS FOR CYANOBACTERIAL BIOFDLMS MONITORING IN HYPOGEAN MONUMENTS.
- Author
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QUINTANA, J. CALVO, PERMARINI, S., ALBERTANO, P., PALLESCHI, G., and MOSCONE, D.
- Subjects
CYANOBACTERIA ,BIOFILMS ,POTASSIUM ,CALCIUM ,POTENTIOMETERS - Published
- 2002
112. BIENZYME REACTOR FOR ELECTROCHEMICAL DETECTION OF LOW CONCENTRATIONS OF URIC-ACID AND GLUCOSE
- Author
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ELEKES, O, MOSCONE, D, VENEMA, K, and KORF, J
- Subjects
ENZYME REACTOR ,AMPEROMETRY ,BLOOD ,URIC ACID ,MEDIATOR, ASCORBIC ACID ,ELECTRODES ,MICRODIALYSIS ,URATE ,GLUCOSE - Abstract
An enzyme-based flow-injection amperometric analysis system (FIA) for monitoring of uric acid and glucose is described, The oxidase and peroxidase enzymes are physically co-immobilised in a sandwich-type reactor and ferrocene serves as a mediator. The assays are based on the measurement of a reduction current resulting from the enzymatic reactions, at a glassy carbon electrode held at 0.00 mV (vs, Ag/AgCl). The high selectivity (ascorbic acid did not interfere) is coupled to high sensitivity (a detection limit of 30 and 60 nmol/l for uric acid and glucose, respectively; signal/noise = 3) and good stability (the enzymes remained active for more than 6 weeks at 30 degrees C), The usefulness of the assay in clinical chemistry is illustrated by the measurement of human serum uric acid and glucose concentration. The results obtained were in fairly good agreement with those obtained using conventional hospital laboratory methods.
- Published
- 1995
113. Electrochemical Biocells for continuos monitong of aminoacids in food
- Author
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Palleschi, G., Bertocchi, P., Compagnone, Dario, Lavagnini, M. G., Moscone, D., and Volpe, G.
- Published
- 1994
114. Enzyme electrode probes for determination of metabolites in biological fluids
- Author
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Palleschi, G, Bernabei, M, Bertocchi, P, Compagnone, Dario, Lavagnini, M. G., and Moscone, D.
- Published
- 1993
115. AFB1-AP Conjugate for Enzyme Immunoassay of Aflatoxin B1 in Corn Samples.
- Author
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Neagu, D., Capodilupo, A., Vilkanauskyte, A., Micheli, L., Palleschi, G., and Moscone, D.
- Subjects
AFLATOXINS ,MYCOTOXIN synthesis ,ENZYME-linked immunosorbent assay ,CORN analysis ,ALKALINE phosphatase ,TOXINS - Abstract
This article describes the conjugation between aflatoxin B1 (AFB1), one of the major mycotoxins, and alkaline phosphatase (AP), one of the most used enzymes for immunoassays. In addition, an application of the ELISA method for aflatoxin B1 determination in corn is presented. Three AFB1-AP conjugates in different toxin-enzyme ratios were prepared and tested. The ELISA results, developed with the most effective conjugate obtained, showed a satisfactory working range between 2.4 and 4000 ng of toxin/g of corn. The detection limit was 2 ng/g in corn samples, and recoveries ranged from 105 to 120%. [ABSTRACT FROM AUTHOR]
- Published
- 2009
- Full Text
- View/download PDF
116. Ex Vivo Continuous Glucose Monitoring With Microdialysis Technique: The Example of GlucoDay.
- Author
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Ricci, F., Moscone, D., and Palleschi, G.
- Abstract
The use of a glucose biosensor in conjunction with a microdialysis probe used to sample the interstitial fluid of the patient has been demonstrated to be extremely useful and advantageous for obtaining a continuous glucose monitoring instrument capable of detecting glycemic level in real time for a long period. The first example of this kind of instrument which was cleared for commercialization is the GlucoDay made by Menarini. The approach used by the GlucoDay presents several advantages if compared with other instruments and its features and future prospective are thoroughly discussed. [ABSTRACT FROM PUBLISHER]
- Published
- 2008
- Full Text
- View/download PDF
117. Rapid Screening Electrochemical Methods for Aflatoxin B1 and Type-A Trichothecenes: A Preliminary Study.
- Author
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Piermarini, S., Volpe, G., Ricci, F., Micheli, L., Moscone, D., Palleschi, G., Führer, M., Krska, R., and Baumgartner, S.
- Subjects
AFLATOXINS ,TRICHOTHECENES ,ALKALINE phosphatase ,PEROXIDASE ,MICROPLATES ,ENZYME-linked immunosorbent assay - Abstract
In this work are presented methods for detection of aflatoxin B1 and type-A trichothecenes, based on the use of indirect competitive ELISA format coupled with a 96-well screen-printed microplate. Electrochemical immunoassays for AFB1, T-2, and HT-2 were performed and the activity of the alkaline phosphatase or horseradish peroxidase labeled enzymes were measured using intermittent pulse amperometry (IPA) as electrochemical technique. Using standard solutions of the target analyte the LOD of the assays were 0.3 and 0.2 ng ml-1 for T-2 and AFB1 respectively, while the sensitivity was 1.2 ng ml-1 for both. For Aflatoxin B1, a stability study of electrochemical plate was also performed. Moreover, the matrix effect was evaluated using two different extraction treatments from corn. [ABSTRACT FROM AUTHOR]
- Published
- 2007
- Full Text
- View/download PDF
118. Development of SYBR‐Green Real‐Time PCR and a Multichannel Electrochemical Immunosensor for Specific Detection of Salmonella enterica.
- Author
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Delibato, E., Volpe, G., Stangalini, D., De Medici, D., Moscone, D., and Palleschi, G.
- Subjects
POLYMERASE chain reaction ,SALMONELLA ,DNA ,MONOCLONAL antibodies ,ELECTROCHEMISTRY - Abstract
The objective of the present work was to develop and evaluate an SYBR Green real time polymerase chain reaction (PCR) method for the specific detection of Salmonella spp in broth cultures and meat samples experimentally contaminated. Also, a simple and rapid multichannel electrochemical immunosensor (MEI) for this pathogen is under study. The PCR was carried out using primers ttr6 and ttr4 for the amplification of a highly conserved DNA region ( ttr sequence) specific for all Salmonella serovars. A boiling step, for the extraction of DNA, was combined with a real time PCR method based on the double‐stranded DNA (dsDNA) binding dye SYBR Green. The standard curve constructed using the mean threshold cycle and various concentrations of S. enteritidis (ranging fron 10 2 to 10 8 CFU mL -1 ) showed good linearity (R 2 =0.999) with the minimum level of detection of 10 2 CFU mL -1 . The experiments were conducted analyzing 30 Salmonella strains and 20 non‐ Salmonella strains. All Salmonella serotypes tested were ttr ‐positive and all other bacteria yielded no amplification products. The specificity of the reaction was confirmed by the melting temperature (T m ) of the amplicon obtained (T m =80.1±0.1). To verify the effectiveness of the assay, experiments were conducted on experimentally contaminated samples, which were also analyzed for comparison by the standard cultural method. A multichannel electrochemical immunosensor for detection of Salmonella also was developed. It consists of a disposable screen‐printed sensor array, coupled with a multichannel pulse monitor, which was assembled as an immunosensor through the use of specific monoclonal (MAb) and polyclonal (PAb) antibodies in a sandwich format. The limit of detection was calculated to be 2×10 6 UFC mL -1 with a working range between 5×10 6 to 5×10 8 UFC mL -1 and a total analysis time of about 3 h. This immunoelectrochemical system is economical, rapid, and easy to use but it is still under development in order to improve its analytical performance. [ABSTRACT FROM AUTHOR]
- Published
- 2006
- Full Text
- View/download PDF
119. Detection of Aflatoxin B 1 in Barley: Comparative Study of Immunosensor and HPLC.
- Author
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Ammida, N.H. S., Micheli, Lawa, Piermarini, S., Moscone, D., and Palleschi, G.
- Subjects
ENZYME-linked immunosorbent assay ,AFLATOXINS ,CARBON electrodes ,MONOCLONAL antibodies ,SERUM albumin ,HIGH performance liquid chromatography - Abstract
In the present work, an indirect competitive electrochemical enzyme linked immunosorbent assay (ELISA) has been used for determination of aflatoxin B 1 (AFB 1 ) in barley. The method involves the use of disposable screen‐printed carbon electrodes (SPCEs) and anti‐aflatoxin B 1 monoclonal antibodies (MAb) for immunosensor development. The specificity of the assay was assessed by studying the cross‐reactivity of the MAb relative to AFB 1 . The results indicated that the MAb could readily distinguish AFB 1 from other toxins, with the exception of AFG 1 . The stability of the coating reagents was evaluated using SPCEs coated with AFB 1 ‐bovine serum albumin (BSA) conjugate. The results showed that the coated electrodes could be used for up to one month after their preparation and storage at 4°C. Prior to evaluating the performance of the electrochemical immunosensor for AFB 1 with spiked samples, the effect of barley extract on assay performance was tested. Using this calibration method, the limit of detection (LOD) was found to be 90 pg mL -1 . The linear range was 0.1–10 ng mL -1 , and recoveries ranged from 100%–125%. The results obtained were confirmed by high performance liquid chromatography (HPLC) coupled with fluorescence detection. These results demonstrated the suitability of the proposed method for routine screening of AFB 1 in barley. [ABSTRACT FROM AUTHOR]
- Published
- 2006
- Full Text
- View/download PDF
120. Development and Comparative Evaluation of Different Screening Methods for Detection of Staphylococcus aureus.
- Author
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Delibato, E., Bancone, M., Volpe, G., De Medici, D., Moscone, D., and Palleschi, G.
- Subjects
STAPHYLOCOCCUS aureus ,IMMUNOGLOBULIN G ,DNA polymerases ,ALKALINE phosphatase ,ENZYME-linked immunosorbent assay ,STAPHYLOCOCCUS - Abstract
Different ELISA tests to detect and quantify levels of S. aureus in broth cultures were developed and compared. In all cases the assays were a modification of a "sandwich" format based on the use of common IgG as well as specific antibodies to bind protein A, an antigen localized in the cellular wall of S. aureus and partially extracted by boiling. Initially, human IgG was immobilized on the surface of microtitre plate wells in order to bind, by means of the Fc region, protein A that was present either in standard solutions or broth cultures of S. aureus treated by a boiling step. The sandwich format was completed using monoclonal (MAb) antibodies specific for protein A. The amount of bound antibody was evaluated using antiglobulins labelled with alkaline phosphatase (Ab
2 -AP). Reading the absorbance at 405 nm a detection limit (LOD) of 0.6ng/mL and 2 × 106 CFU/mL was found for protein A and for S. aureus, respectively. In order to improve the performance of the immunoassay, different approaches were pursued: an enzymatic amplification system (Ampli Q); the use of immunomagnetic beads employed both in a colorimetric (ELIMC Enzyme-Linked Immunomagnetic Colorimetric) and in an electrochemical (ELIME = Enzyme-Linked Immunomagnetic Electrochemistry) assay. Using these systems the detection limit decreased by a factor of about 30-fold for Ampli Q and ELIMC, and about 2000-fold for ELIME formats. In addition, a qualitative polymerase chain reaction (PCR) method, using nuc gene primers, was set up and performed in parallel and its various parameters were optimized. This method was able to detect 102 CFU/mL. In terms of minimum detectable concentration of S. aureus and total analysis time, the performance of the PCR assay and ELIME, turned out to be comparable. [ABSTRACT FROM AUTHOR]- Published
- 2005
- Full Text
- View/download PDF
121. Nonconducting polymers on Prussian Blue modified electrodes: improvement of selectivity and stability of the advanced H2O2 transducer.
- Author
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Lukachova, L.V., Kotel'nikova, E.A., D'Ottavi, D., Shkerin, E.A., Karyakina, E.E., Moscone, D., Palleschi, G., Curulli, A., and Karyakin, A.A.
- Abstract
An approach to improve the analytical performance of a Prussian Blue (PB)-based hydrogen peroxide transducer is described. In support of this objective, both the stabilizing and anti-interferent properties of nonconducting films were used. Electropolymerization on the top surface of PB modified electrodes is possible due to the high oxidizing ability of Berlin Green, and the growth of nonconductive polymers may be independently monitored by investigating the redox activity of the inorganic polycrystal. The best performance characteristics, which are advantageous over existing H2O2 sensors, were obtained for PB electrodes covered with electropolymerized o-phenylenediamine (1,2-diaminobenzene). The reported transducer remained at the 100% response state for more than 20 h under continuous flow of 0.1-mM hydrogen peroxide (flow rate 1 mlmin-1), which improves the stability level among the selective H2O2 sensors by one order of magnitude. The selectivity factor of the PB-poly (1,2-diaminobenzene) based transducer relative to ascorbate is nominally 600. PB-poly(1,2-diaminobenzene) modified electrode allows the detection hydrogen peroxide in the flow-injection mode down to 10-7 M with sensitivity of 0.3 AM-1cm-2, which is two times lower compared to the uncovered PB-based transducer. [ABSTRACT FROM PUBLISHER]
- Published
- 2003
- Full Text
- View/download PDF
122. New electrochemical sensors for detection of nitrites and nitrates
- Author
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Badea, M., Amine, A., Palleschi, G., Moscone, D., Volpe, G., and Curulli, A.
- Published
- 2001
- Full Text
- View/download PDF
123. Amperometric separation-free immunosensor for real-time environmental monitoring
- Author
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Killard, A. J., Micheli, L., Grennan, K., Franek, M., Kolar, V., Moscone, D., Palchetti, I., and Smyth, M. R.
- Published
- 2000
- Full Text
- View/download PDF
124. Monitoring alcoholic fermentation of red wine by electrochemical biosensors
- Author
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Esti, M., Volpe, G., Dario Compagnone, Mariotti, G., Moscone, D., and Palleschi, G.
- Subjects
Red winemaking ,alcoholic fermentation ,Settore CHIM/01 - Chimica Analitica ,ethanol ,glycerol ,Horticulture ,glucose ,electrochemical biosensors ,fructose ,Food Science
125. Effect of photosynthesis on pH variation in cyanobacterial biofilms from Roman catacombs
- Author
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Albertano, P., LAURA BRUNO, D Ottavi, D., Moscone, D., and Palleschi, G.
- Subjects
Settore CHIM/01 - Chimica Analitica
126. New electrochemical sensors used in flow injection analysis for nitrite/nitrate determination
- Author
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Badea, M., Antonella CURULLI, Dǎnet, A., Moscone, D., and Palleschi, G.
- Subjects
Settore CHIM/01 - Chimica Analitica
127. Biosensors for quality and safety control of olive oil: A review
- Author
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Arduini, F., Amine, A., Moscone, D., and Giuseppe Palleschi
128. Construction and evaluation of a needle glucose probe for in vivo monitoring
- Author
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Bugli, M., Moscone, D., Rodino, P., and Giuseppe Palleschi
- Subjects
Settore CHIM/01 - Chimica Analitica
129. Biosensors for quality and safety control of olive oil: A review
- Author
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FABIANA ARDUINI, Amine, A., Moscone, D., and Palleschi, G.
- Subjects
Settore CHIM/01 - Chimica Analitica
130. Oxygen concentration, nitrogenase activity and heterocyst frequency in the leaf cavities of Azolla filiculoides Lam
- Author
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Grilli Caiola, M., Canini, A., and Moscone, D.
- Abstract
Oxygen concentration within the leaf cavities from the apex to the base of Azolla filiculoides Lam. was measured by an oxygen microprobe. The oxygen values in the leaf cavities were lower than in the aerated water and were within the range 0.157–0.183 atm. At 140 µE m−2 s−1 irradiance, oxygen decreased in the leaf cavities where acetylene reduction activity and heterocyst frequency were highest. Oxygen evolution and oxygen consumption inhibition by KCN strongly suggest that oxygen concentration changes, in the leaf cavities, are due to both photosynthesis of Azolla and Anabaena and to respiration of the whole system.
- Published
- 1989
- Full Text
- View/download PDF
131. Electroanalytical Sensor Based on Gold-Nanoparticle-Decorated Paper for Sensitive Detection of Copper Ions in Sweat and Serum
- Author
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Simona Roggero, Neda Bagheri, Paolo A. Netti, Vincenzo Mazzaracchio, Stefano Cinti, Danila Moscone, Noemi Colozza, Mohammad Saraji, Fabiana Arduini, Concetta Di Natale, Bagheri, N., Mazzaracchio, V., Cinti, S., Colozza, N., Di Natale, C., Netti, P. A., Saraji, M., Roggero, S., Moscone, D., and Arduini, F.
- Subjects
Analyte ,Microfluidics ,Metal Nanoparticles ,Nanoparticle ,chemistry.chemical_element ,Nanotechnology ,Biosensing Techniques ,Standard solution ,010402 general chemistry ,01 natural sciences ,Analytical Chemistry ,law.invention ,Biosensing Technique ,law ,Ion ,Sweat ,Ions ,Filter paper ,010401 analytical chemistry ,Copper ,0104 chemical sciences ,chemistry ,Reagent ,Gold ,Atomic absorption spectroscopy - Abstract
The growth of (bio)sensors in analytical chemistry is mainly attributable to the development of affordable, effective, portable, and user-friendly analytical tools. In the field of sensors, paper-based devices are gaining a relevant position for their outstanding features including foldability, ease of use, and instrument-free microfluidics. Herein, a multifarious use of filter paper to detect copper ions in bodily fluids is reported by exploiting this eco-friendly material to (i) synthesize AuNPs without the use of reductants and/or external stimuli, (ii) print the electrodes, (iii) load the reagents for the assay, (iv) filter the gross impurities, and (v) preconcentrate the target analyte. Copper ions were detected down to 3 ppb with a linearity up to 400 ppb in standard solutions. The applicability in biological matrices, namely, sweat and serum, was demonstrated by recovery studies and by analyzing these biofluids with the paper-based platform and the reference method (atomic absorption spectroscopy), demonstrating satisfactory accuracy of the novel eco-designed analytical tool.
- Published
- 2021
132. Paper-based electroanalytical strip for user-friendly blood glutathione detection
- Author
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Valeria Manovella, Stefano Cinti, Danila Moscone, Fabiana Arduini, Nicolò Interino, Maria Rita Tomei, Tomei, M. R., Cinti, S., Interino, N., Manovella, V., Moscone, D., and Arduini, F.
- Subjects
Blood, Reagent-free, Screen-printed electrodes, Self-care, Wax printing ,Materials science ,Screen-printed electrodes ,02 engineering and technology ,Overpotential ,010402 general chemistry ,01 natural sciences ,chemistry.chemical_compound ,Conductive ink ,Materials Chemistry ,Settore CHIM/01 - Chimica Analitica ,Electrical and Electronic Engineering ,Process engineering ,Instrumentation ,Detection limit ,Reagent-free ,Prussian blue ,Nanocomposite ,Filter paper ,business.industry ,Metals and Alloys ,Repeatability ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,0104 chemical sciences ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Blood ,chemistry ,Screen-printed electrode ,Self-care ,0210 nano-technology ,business ,Sensitivity (electronics) ,Wax printing - Abstract
Paper-based devices are always more gaining a relevant position in the field of sensors. The continuous demand for affordable, simple, sustainable, and portable devices, is making paper as the ideal basis towards the realization of analytical tools for the easy self-testing. In this work, we demonstrate, for the first time, the development of a disposable paper-based printed electroanalytical strip for reliable, rapid, and high-throughput detection of glutathione in blood. The detection is based on the thiol-disulfide exchange reaction, which produces a detectable compound easily oxidizable at a Prussian Blue/carbon black nanocomposite involving a favorable low-interference overpotential. This nanocomposite is mixed within a carbon-based conductive ink and successively screen-printed onto a wax-patterned filter paper. The employment of paper provides a reagent-free device, as a consequence of the reagents pre-loading within the testing area. After the experimental conditions have been optimized, glutathione has been detected up to 10 mM, with a detection limit of 60 μM, and a sensitivity of (0.102 ± 0.005) μA/mM. This sensor showed satisfactory repeatability (relative standard deviation equal to 10%, for detection of glutathione 1 mM), especially by considering the hand-made manufacturing process. The “real-world” applicability of this strip has been evaluated by quantifying blood glutathione at physiological levels and by recovery studies achieving satisfactory values.
- Published
- 2019
133. Preparation of paper-based devices for reagentless electrochemical (bio)sensor strips
- Author
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Fabiana Arduini, Stefano Cinti, Danila Moscone, Cinti, S., Moscone, D., and Arduini, F.
- Subjects
Paper ,Computer science ,Nanotechnology ,Biosensing Techniques ,STRIPS ,Reference electrode ,General Biochemistry, Genetics and Molecular Biology ,Phosphates ,law.invention ,03 medical and health sciences ,Software portability ,Silver chloride ,chemistry.chemical_compound ,Settore CHIM/01 ,0302 clinical medicine ,law ,Electrodes ,030304 developmental biology ,0303 health sciences ,Electrochemical Techniques ,Equipment Design ,chemistry ,Filter (video) ,Electrode ,Screen printing ,Biosensor ,030217 neurology & neurosurgery - Abstract
Despite substantial advances in sensing technologies, the development, preparation, and use of self-testing devices is still confined to specialist laboratories and users. Decentralized analytical devices will enormously impact daily lives, enabling people to analyze diverse clinical, environmental, and food samples, evaluate them and make predictions to improve quality of life, particularly in remote, resource-scarce areas. In recent years, paper-based analytical tools have attracted a great deal of attention; the well-known properties of paper, such as abundance, affordability, lightness, and biodegradability, combined with features of printed electrochemical sensors, have enabled the development of sustainable devices that drive (bio)sensors beyond the state of the art. Their blindness toward colored/turbid matrices (i.e., blood, soil), their portability, and the capacity of paper to autonomously filter/purge/react with target species make such devices powerful in establishing point-of-need tools for use by non-specialists. This protocol describes the preparation of a voltammetric phosphate sensor and an amperometric nerve agent biosensor; both platforms produce quantitative measurements with currents in the range of microamperes. These printed strips comprise three electrodes (graphite for working and counter electrodes and silver/silver chloride (Ag/AgCl) for the reference electrode) and nanomodifiers (carbon black and Prussian blue) to improve their performance and specificity. Depending on analytical need, different types of paper (filter, office) and configurations (1D, 2D, 3D) can be adopted. The protocol, based on the use of cost-effective manufacturing techniques such as drop casting (to chemically modify the substrate surface) and wax/screen printing (for creating the channels and electrodes), can be completed in
- Published
- 2019
134. Multi-array wax paper-based platform for the pre-concentration and determination of silver ions in drinking water
- Author
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Eleonora Nobile, Neda Bagheri, Danila Moscone, Stefano Cinti, Fabiana Arduini, Bagheri, N., Cinti, S., Nobile, E., Moscone, D., and Arduini, F.
- Subjects
Paper-based ,Inorganic chemistry ,Nanoparticle ,02 engineering and technology ,01 natural sciences ,Analytical Chemistry ,Ion ,chemistry.chemical_compound ,Drinking water ,Detection limit ,Wax ,Prussian blue ,Chemistry ,010401 analytical chemistry ,Repeatability ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Pre-concentration ,Reagent ,visual_art ,Silver ion ,visual_art.visual_art_medium ,0210 nano-technology ,Pre concentration ,Wax printing - Abstract
In this work, a wax-patterned chromatographic paper has been utilized as a holistic platform to 1) synthesize Prussian Blue Nanoparticles (sensing species), 2) load the reagents for the assay, 3) concentrate the sample through multistep, and 4) visualize the determination of silver ions. Waters are continuously affected by changes in the composition, thus the utilization of reagent-free analytical tools is of huge interest for smart drinking water monitoring. Herein, we report the characterization and application of a multi-array paper-based platform for the colorimetric determination of silver ions based on the conversion from Prussian Blue to its silver-based analogue, namely Ag4[Fe(CN)6]. In particular, the platform highlights the increase of sensitivity due to paper pre-concentration of sample, that can be easily adapted to the analytical necessities. Within the proposed experimental setup, Ag+ is visualized down to a detection limit of 0.9 μM, with high repeatability and satisfactory recoveries in the range comprised between 90 and 113%.
- Published
- 2021
135. Paper-based synthesis of Prussian Blue Nanoparticles for the development of whole blood glucose electrochemical biosensor
- Author
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Danila Moscone, Fabiana Arduini, Roberto Cusenza, Stefano Cinti, Cinti, S., Cusenza, R., Moscone, D., and Arduini, F.
- Subjects
Blood Glucose ,Paper ,Surface Properties ,Reducing agent ,Paper-based ,Electrode ,Surface Propertie ,Nanoparticle ,Nanotechnology ,Biosensing Techniques ,02 engineering and technology ,01 natural sciences ,Analytical Chemistry ,Biosensing Technique ,chemistry.chemical_compound ,Settore CHIM/01 - Chimica Analitica ,Glucose oxidase ,Particle Size ,Hydrogen peroxide ,Electrodes ,Prussian blue ,Electrochemical Technique ,biology ,Filter paper ,010401 analytical chemistry ,Electrochemical Techniques ,Hydrogen-Ion Concentration ,021001 nanoscience & nanotechnology ,BiosensorPaper-basedPoint-of-carePrussian Blue NanoparticlesScreen-printed electrodesWhole blood ,Environmentally friendly ,0104 chemical sciences ,Whole blood ,Prussian Blue Nanoparticle ,chemistry ,Point-of-care ,Screen-printed electrode ,biology.protein ,Nanoparticles ,0210 nano-technology ,Biosensor ,Ferrocyanide ,Ferrocyanides - Abstract
Nowadays, environmentally friendly synthesis pathways for preserving the environment and minimizing waste are strongly required. Herein, we propose filter paper as a convenient scaffold for chemical reactions. To demonstrate this novel approach, Prussian Blue Nanoparticles (PBNPs) were synthesized on filter paper by utilizing few μL of its precursors without external inputs, i.e. pH, voltage, reducing agents, and without producing waste as well. The functional paper, named “Paper Blue”, is successfully applied in the sensing field, exploiting the reduction of hydrogen peroxide at low applied potential. The eco-designed “Paper Blue” was combined with wax- and screen-printing to manufacture a reagentless electrochemical point-of-care device for diabetes self-monitoring, by using glucose oxidase as the biological recognition element. Blood glucose was linearly detected for a wide concentration range up to 25 mM (450 mg/dL), demonstrating its suitability for management of diabetes and glucose-related diseases. The Paper Blue-based biosensor demonstrated a correlation coefficient of 0.987 with commercial glucose strips (Bayer Contour XT). The achieved results demonstrated the effectiveness of this approach, which is also extendible to other (bio)systems to be applied in catalysis, remediation, and diagnostics.
- Published
- 2018
136. Carbon black as an outstanding and affordable nanomaterial for electrochemical (bio)sensor design
- Author
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Vincenzo Mazzaracchio, Stefano Cinti, Danila Moscone, Aziz Amine, Viviana Scognamiglio, Fabiana Arduini, Arduini, F., Cinti, S., Mazzaracchio, V., Scognamiglio, V., Amine, A., and Moscone, D.
- Subjects
Paper ,Immunosensors ,DNA sensors ,Materials science ,Nanostructure ,Electrode ,Microfluidics ,Biomedical Engineering ,Biophysics ,DNA sensor ,Nanotechnology ,Enzymatic biosensors ,Biosensing Techniques ,02 engineering and technology ,Immunosensor ,01 natural sciences ,Paper-based biosensor ,Nanomaterials ,Carbon-based nanomaterial ,Biosensing Technique ,Settore CHIM/01 ,Soot ,Carbon based nanomaterials ,Electrochemistry ,Animals ,Humans ,Paper-based biosensors ,Electrodes ,Electrochemical Technique ,Animal ,010401 analytical chemistry ,Electrochemical Techniques ,General Medicine ,Carbon black ,Equipment Design ,021001 nanoscience & nanotechnology ,Nanostructures ,0104 chemical sciences ,Carbon-based nanomaterials ,Enzymatic biosensor ,Bio sensor ,0210 nano-technology ,Biosensor ,Biotechnology ,Human - Abstract
Advances in cutting-edge technologies including nanotechnology, micmfluidics, electronic engineering, and material science have boosted a new era in the design of robust and sensitive biosensors. In recent years, carbon black has been re-discovered in the design of electrochemical (bio)sensors thanks to its interesting electroanalytical properties, absence of treatment requirement, cost-effectiveness (c.a. 1 (sic)/Kg), and easiness in the preparation of stable dispersions. Herein, we present an overview of the literature on carbon black-based electrochemical (bio)sensors, highlighting current trends and possible challenges to this rapidly developing area, with a special focus on the fabrication of carbon black-based electrodes in the realisation of sensors and biosensors (e.g. enzymatic, immunosensors, and DNA-based).
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- 2020
137. Paper-based electrochemical peptide nucleic acid (PNA) biosensor for detection of miRNA-492: a pancreatic ductal adenocarcinoma biomarker
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Danila Moscone, Stefano Cinti, Concetta Avitabile, Maria Moccia, Veronica Caratelli, Fabiana Arduini, Anna Lisa Imbriani, Biagio Pede, Michele Saviano, Moccia, M., Caratelli, V., Cinti, S., Pede, B., Avitabile, C., Saviano, M., Imbriani, A. L., Moscone, D., and Arduini, F.
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Peptide Nucleic Acids ,Serum ,Pancreatic ductal adenocarcinoma ,Biomedical Engineering ,Biophysics ,02 engineering and technology ,Biosensing Techniques ,Adenocarcinoma ,01 natural sciences ,chemistry.chemical_compound ,Settore CHIM/01 ,microRNA ,Ruthenium (III) hexamine ,Electrochemistry ,medicine ,Humans ,Screen-printing ,Peptide nucleic acid ,010401 analytical chemistry ,General Medicine ,Electrochemical Techniques ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Electrochemical gas sensor ,MicroRNAs ,Wax-printing ,medicine.anatomical_structure ,chemistry ,Duplex (building) ,Cancer research ,Differential pulse voltammetry ,0210 nano-technology ,Pancreas ,Biosensor ,Biomarkers ,Biotechnology - Abstract
Pancreatic ductal adenocarcinoma is the predominant neoplastic disease of the pancreas and it represents the fourth most frequent cause of death in cancer-related disease, with only 8% of survivors after 5-year to the diagnosis. The main issues of this type of cancer rely on fast progress (i.e. 14 months from T1 to a T4 stage), nonspecific symptoms with delay in diagnosis, and the absence of effective screening strategies. To address the lack of early diagnosis, we report a cost-effective paper-based biosensor for the detection of miRNA-492, which is recognised as a biomarker for pancreatic ductal adenocarcinoma. To design a miniaturised, sensitive, and robust paper-based platform, an electrochemical sensor was screen-printed on office paper previously wax-patterned via wax-printing technique. The paper-based sensor was then engineered with a novel and highly specific peptide nucleic acid (PNA) as the recognition element. The formation of PNA/miRNA-492 adduct was evaluated by monitoring the interaction between the positively charged ruthenium (III) hexamine with uncharged PNA and/or negatively charged PNA/miRNA-492 duplex by differential pulse voltammetry. The paper-based biosensor provided a linear range up to 100 nM, with a LOD of 6 nM. Excellent selectivity towards one- and two-base mismatches (1MM, 2MM) or scrambled (SCR) sequences was highlighted and the applicability for biomedical analyses was demonstrated, measuring miRNA-492 in undiluted serum samples.
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- 2020
138. Experimental Comparison in Sensing Breast Cancer Mutations by Signal ON and Signal OFF Paper-Based Electroanalytical Strips
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Emily P. Nguyen, Fabiana Arduini, Claudio Parolo, Giulia Cinotti, Danila Moscone, Stefano Cinti, Arben Merkoçi, Veronica Caratelli, Cinti, S., Cinotti, G., Parolo, C., Nguyen, E. P., Caratelli, V., Moscone, D., Arduini, F., and Merkoci, A.
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Paper ,DNA, Single-Stranded ,Breast Neoplasms ,STRIPS ,Biosensing Techniques ,010402 general chemistry ,computer.software_genre ,01 natural sciences ,Signal ,Field (computer science) ,Analytical Chemistry ,law.invention ,Biosensing Technique ,DNA-based biosensors ,Breast cancer ,Settore CHIM/01 ,Design and Development ,law ,Experimental comparison ,Detection methods ,medicine ,Humans ,Liquid biopsy ,Protocol (science) ,Electrochemical Technique ,Chemistry ,010401 analytical chemistry ,Analytical performance ,Electrochemical Techniques ,medicine.disease ,Signal on ,0104 chemical sciences ,Emerging technologies ,Mutation ,Single strand DNA ,Female ,Data mining ,Detection protocols ,Biosensor ,computer ,Breast Neoplasm ,Human - Abstract
Altres ajuts: the ICN2 is funded by the CERCA Programme/Generalitat de Catalunya. The development of paper-based electroanalytical strips as powerful diagnostic tools has gained a lot of attention within the sensor community. In particular, the detection of nucleic acids in complex matrices represents a trending topic, especially when focused toward the development of emerging technologies, such as liquid biopsy. DNA-based biosensors have been largely applied in this direction, and currently, there are two main approaches based on target/probe hybridization reported in the literature, namely Signal ON and Signal OFF. In this technical note, the two approaches are evaluated in combination with paper-based electrodes, using a single strand DNA relative to H1047R (A3140G) missense mutation in exon 20 in breast cancer as the model target. A detailed comparison among the analytical performances, detection protocol, and cost associated with the two systems is provided, highlighting the advantages and drawbacks depending on the application. The present work is aimed to a wide audience, particularly for those in the field of point-of-care, and it is intended to provide the know-how to manage with the design and development stages, and to optimize the platform for the sensing of nucleic acids using a paper-based detection method.
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- 2019
139. A challenge in biosensors: Is it better to measure a photon or an electron for ultrasensitive detection?
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Patrizia Simoni, Martina Zangheri, Elisa Marchegiani, Laura Fabiani, Aldo Roda, Noemi Colozza, Danila Moscone, Mara Mirasoli, Fabiana Arduini, Roda A., Arduini F., Mirasoli M., Zangheri M., Fabiani L., Colozza N., Marchegiani E., Simoni P., and Moscone D.
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Chemiluminescence ,Biomedical Engineering ,Biophysics ,Amperometry ,Reproducibility of Result ,Nanotechnology ,Electrons ,02 engineering and technology ,Biosensing Techniques ,Immunosensor ,Electron ,01 natural sciences ,Horseradish peroxidase ,Sensitivity and Specificity ,law.invention ,Biosensing Technique ,Settore CHIM/01 ,law ,Electrochemistry ,Electrochemical biosensor ,Enzyme-based biosensor ,Photons ,Electrochemical Technique ,biology ,Chemistry ,Paper-based assay ,010401 analytical chemistry ,Reproducibility of Results ,General Medicine ,Electrochemical Techniques ,021001 nanoscience & nanotechnology ,Photon ,0104 chemical sciences ,Luminescent Measurement ,Luminescent Measurements ,biology.protein ,0210 nano-technology ,Biosensor ,Biotechnology - Abstract
Biosensor development exploiting various transduction principles is characterized by a strong competition to reach high detectability, portability and robustness. Nevertheless, a literature-based comparison is not possible, as different conditions are employed in each paper. Herein, we aim at evaluating which measurement, photons or electrons, yields better biosensor performance. Upon outlining an update in recent achievements to boost analytical performance, amperometry and chemiluminescence (CL)-based biosensors are directly compared employing the same biospecific reagents and analytical formats. Horseradish peroxidase (HRP) and hydrogen peroxide concentrations were directly measured, while glucose and mouse IgG were detected employing an enzyme paper-based biosensor and an immunosensor, respectively. Detectability was down to picomoles of hydrogen peroxide (4 pmol for CL and 210 pmol for amperometry) and zeptomoles of HRP (45 zmol for CL and 20 zmol for amperometry); IgG was detected down to 12 fM (CL) and 120 fM (amperometry), while glucose down to 17 μM (CL) and 40 μM (amperometry). Results showed that amperometric and CL biosensors offered similar detectability and analytical performance, with some peculiarities that suggest complementary application fields. As they generally provided slightly higher detectability and wider dynamic ranges, CL-based biosensors appear more suitable for point-of-care testing of clinical biomarkers, where detectability is crucial. Nevertheless, as high detectability in CL biosensors usually requires longer acquisition times, their rapidity will allocate electrochemical biosensors in real-time monitoring and wearable biosensors. The analytical challenge demonstrated that these biosensors have competitive and similar performance, and between photons and electrons the competition is still open.
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- 2019
140. A 96-well wax printed Prussian Blue paper for the visual determination of cholinesterase activity in human serum
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Fabiana Arduini, Danila Moscone, Renato Massoud, Mohammad Saraji, Stefano Cinti, Neda Bagheri, Veronica Caratelli, Bagheri, N., Cinti, S., Caratelli, V., Massoud, R., Saraji, M., Moscone, D., and Arduini, F.
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Paper ,Computer science ,Biomedical Engineering ,Biophysics ,Biosensing Techniques ,02 engineering and technology ,01 natural sciences ,Biosensing Technique ,chemistry.chemical_compound ,Limit of Detection ,Activity detection ,Electrochemistry ,Humans ,Settore CHIM/01 - Chimica Analitica ,Paper-based assay, Colorimetric detection, Multiplexed measurement, Butyrylcholinesterase, Chromatography paper ,Waxe ,Coloring Agent ,Coloring Agents ,Chromatography paper ,Detection limit ,Prussian blue ,Chromatography ,Paper-based assay ,010401 analytical chemistry ,Equipment Design ,General Medicine ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Thiocholine ,chemistry ,Waxes ,Butyrylcholinesterase ,Printing, Three-Dimensional ,Colorimetry ,0210 nano-technology ,Multiplexed measurement ,Ferrocyanide ,Colorimetric detection ,Human ,Ferrocyanides ,Biotechnology - Abstract
In the last decades, there is a growing search for analytical strategies to ensure clinical analysis without the need of laboratory set-up and skilled personnel. Indeed, user-friendly and low-cost devices are highly valued in the era of sustainability for their capability to be applied in low-resource contexts, such as developing countries. To address this issue, herein we report a 96-well paper-based and laboratory setup-free optical platform for the detection of butyrylcholinesterase enzyme (BChE) activity in human serum. We used chromatographic paper to realize a novel analytical tool exploiting its porous structure for reagentless synthesize Prussian Blue Nanoparticles (the sensing element), as well to load all the reagents required for the measurement. The principle of BChE activity detection relies on the reaction between the enzymatic product thiocholine and Prussian Blue, giving the Prussian White with subsequently Prussian Blue's fading, detected by a common office scanner supported by ImageJ software. Using this novel paper-based optical platform, BChE activity was linearly detected in the 2–15 U/mL range with a detection limit down to 0.8 U/mL. The accuracy was successfully demonstrated by recovery study with spiked serum and by comparing the data with the gold standard method.
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- 2019
141. Origami multiple paper-based electrochemical biosensors for pesticide detection
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Luca Amendola, Stefano Cinti, Danila Moscone, Fabiana Arduini, Veronica Caratelli, Giuseppe Palleschi, Arduini, F., Cinti, S., Caratelli, V., Amendola, L., Palleschi, G., and Moscone, D.
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Paper ,Insecticides ,Biomedical Engineering ,Biophysics ,02 engineering and technology ,Biosensing Techniques ,Standard solution ,Butyrylcholinesterase Alkaline phosphatase Tyrosinase Paraoxon 2,4-dichlorophenoxyacetic acid Atrazine ,01 natural sciences ,Paraoxon ,chemistry.chemical_compound ,Organophosphorus Compounds ,Rivers ,Limit of Detection ,Alkaline phosphatase ,Electrochemistry ,medicine ,Humans ,Settore CHIM/01 - Chimica Analitica ,Atrazine ,Pesticides ,Insecticide ,River ,Prussian blue ,Chromatography ,2,4-dichlorophenoxyacetic acid ,Filter paper ,010401 analytical chemistry ,Water ,General Medicine ,Pesticide ,021001 nanoscience & nanotechnology ,Potentiostat ,0104 chemical sciences ,chemistry ,Butyrylcholinesterase ,Tyrosinase ,Organophosphorus Compound ,0210 nano-technology ,Biosensor ,Butyrylcholinesterase Alkaline phosphatase Tyrosinase Paraoxon 2 ,4-dichlorophenoxyacetic acid Atrazine ,Water Pollutants, Chemical ,Biotechnology ,medicine.drug ,Human - Abstract
Herein, we propose the first three-dimensional origami paper-based device for the detection of several classes of pesticides by combining different enzyme-inhibition biosensors. This device was developed by integrating two different office paper-based screen-printed electrodes and multiple filter paper-based pads to load enzymes and enzymatic substrates. The versatile analysis of different pesticides was carried by folding and unfolding the filter paper-based structure, without any addition of reagents and any sample treatment (i.e. dilution, filtration, pH adjustment). The paper-based platform was employed to detect paraoxon, 2,4-dichlorophenoxyacetic acid, and atrazine by exploiting the capability of these different types of pesticides (i.e. organophosphorus insecticides, phenoxy-acid herbicides, and triazine herbicide) to inhibit butyrylcholinesterase, alkaline phosphatase, and tyrosinase, respectively. The degree of inhibition correlating to the quantity of pesticides was evaluated by chronoamperometrically monitoring the enzymatic activity in the absence and in the presence of pesticides by using a portable potentiostat. To improve the sensitivity, the paper-based electrodes were modified with carbon black alone in the case of platforms for 2,4-dichlorophenoxyacetic acid and atrazine detection, or decorated with Prussian blue nanoparticles for the detection of paraoxon. The paper-based device was applied for the detection of paraoxon, 2,4-dichlorophenoxyacetic acid, and atrazine at ppb level in both standard solutions and river water sample. The accuracy of this origami multiple paper-based electrochemical biosensor was evaluated in river water by recovery studies, obtaining satisfactory values (e.g. for paraoxon 90 ± 1% and 88 ± 2%, for 10 and 20 ppb, respectively). The proposed three-dimensional origami paper device allows for rapid, cost-effective and accurate pesticide detection in surface water as a result of combining filter and office papers, screen-printing, wax-printing and nanomaterial technology.
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- 2019
142. Paper-Based Strips for the Electrochemical Detection of Single and Double Stranded DNA
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Danila Moscone, Federica Casotto, Stefano Cinti, Elena Proietti, Fabiana Arduini, Cinti, S., Proietti, E., Casotto, F., Moscone, D., and Arduini, F.
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Paper ,Electrode ,Reproducibility of Result ,DNA, Single-Stranded ,Metal Nanoparticles ,Nanotechnology ,STRIPS ,010402 general chemistry ,Electrochemistry ,01 natural sciences ,Analytical Chemistry ,law.invention ,chemistry.chemical_compound ,Metal Nanoparticle ,law ,Settore CHIM/01 - Chimica Analitica ,Electrodes ,Kinetic ,Filter paper ,Electrochemical Technique ,Oligonucleotide ,010401 analytical chemistry ,Solid Phase Extraction ,HIV ,Reproducibility of Results ,DNA ,Electrochemical Techniques ,0104 chemical sciences ,Kinetics ,chemistry ,Colloidal gold ,Costs and Cost Analysi ,Costs and Cost Analysis ,Gold ,Double stranded - Abstract
The detection of double stranded DNA (dsDNA) is often associated with the use of laboratory-bound approaches and/or with the prior generation of single stranded DNA (ssDNA), making these methods not suitable for in situ monitoring, i.e., point-of-care diagnostics. Screen-printed technology, coupled to the use of triplex forming oligonucleotides (TFO) as the recognizing probes, offers a great possibility toward the development of portable analytical tools. Moreover, the continuous demand for sustainable processes and waste lowering have highlighted the role of paper-based substrates for manufacturing easy-to-use, low-cost, and sustainable electrochemical devices. In this work, filter paper and copy paper have been utilized to produce E-DNA strips. Gold nanoparticles (AuNPs) have been exploited to immobilize the methylene blue (MB)-tagged TFO and to enhance the charge transfer kinetics at the electrode surface. Both paper-based substrates have been electrochemically characterized, and in addition, the effect of the amount of waxed layers has been evaluated. The paper-based E-DNA strips have been challenged toward the detection of three model targets, obtaining 3 and 7 nM as the detection limit, respectively, for single and double stranded sequences. The repeatability of the manufacturing (homemade) process has been evaluated with a relative standard deviation of approximately 10%. The effectiveness of the filter paper-based platform has been also evaluated in undiluted serum obtaining a similar value of the detection limit (compared to the measurements carried out in buffer solution). In addition, a synthetic PCR amplified dsDNA sequence, related to HIV, has been detected in serum samples.
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- 2018
143. Novel bio-lab-on-a-tip for electrochemical glucose sensing in commercial beverages
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Danila Moscone, Fabiana Arduini, Vincenzo Mazzaracchio, Stefano Cinti, Roberta Marrone, Cinti, S., Marrone, R., Mazzaracchio, V., Moscone, D., and Arduini, F.
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Materials science ,Biomedical Engineering ,Biophysics ,Lab-on-a-tip ,Nanotechnology ,Biosensing Techniques ,02 engineering and technology ,01 natural sciences ,Beverages ,Matrix (chemical analysis) ,Glucose Oxidase ,Electroanalysi ,Settore CHIM/01 ,Electrochemistry ,Miniaturization ,Glucose oxidase ,Beverage ,Electrodes ,Detection limit ,biology ,010401 analytical chemistry ,Pipette ,Electrochemical Techniques ,General Medicine ,Chronoamperometry ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Glucose ,Filter (video) ,biology.protein ,0210 nano-technology ,Biosensor ,Biotechnology - Abstract
The development of portable and user-friendly sensing platforms is a hot topic in the field of analytical chemistry. Among others, electroanalytical approaches exhibit a high amenability for reaching this purpose, i.e. the commercial strips for diabetes care are an obvious success. However, providing fully-integrated and reagent-free methods is always a leitmotiv. In this work, we evaluated the use of a disposable pipette tip, opportunely configured to demonstrate the first example of an electrochemical biosystem in a pipette tip, namely bio-lab-on-a-tip. The combination of a pipette tip, wire electrodes, enzyme, and cotton wool filter, allows the fabrication of a novel electroanalytical platform that does not need expertise-required tasks. To demonstrate the feasibility of this novel method, glucose is detected in beverages by means of chronoamperometry. The experimental setup, entirely built inside the pipette tip, is able to 1) block impurities/interferences from matrix, 2) load/release reagents for the bio-assay, 3) reduce the operating task to zero, and 4) perform electrochemical detection. With optimized experimental parameters, the bio-lab-on-a-tip is able to detect glucose linearly up to 10 mM with a detection limit of 170 μM. The effectiveness of the platform was confirmed by testing commercial beverages, e.g. Coca-Cola and Coca-Cola Zero, with high accuracy. In addition, the shelf-life of the novel device was evaluated, highlighting the role of cotton wool filter for providing a suitable environment for glucose oxidase stability. The novel concept can be easily generalized for further applications in the field of non-invasive clinical diagnostics and in-situ environmental monitoring.
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- 2020
144. Aflatoxin M1 determination and stability study in milk samples using a screen-printed 96-well electrochemical microplate
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Neagu, D., Perrino, S., Micheli, L., Palleschi, G., and Moscone, D.
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AFLATOXINS , *COMPOSITION of milk , *ELECTROCHEMISTRY , *MICROPLATES , *IMMUNOSENESCENCE , *IMMUNOASSAY , *ENZYME-linked immunosorbent assay , *ATOMIC force microscopy - Abstract
Abstract: A highly sensitive method for the determination of aflatoxin M1 (AFM1) is described that involves the use of a disposable multichannel microplate coupled to intermittent pulse amperometry (IPA) for immunosensor development based on a direct competitive assay. Immunoassay parameters were evaluated and optimized to establish an electrochemical enzyme-linked immunosorbent assay (ELISA) procedure for AFM1 in milk samples. The suitability of the immunosensors for the direct analysis of the toxin in milk was assessed. AFM1 was measured with a working range of 5–250 pg mL−1 and a detection limit of 1 pg mL−1. Good recovery values (90–105%) were obtained. The method was used to study the recovery of this toxin in frozen (−30 °C) or lyophilized (4 °C) milk samples stored for up to 3 months. A significant, but variable, decrease (>50%) of the measured AFM1 concentration with respect to the initial toxin contamination levels was found. [Copyright &y& Elsevier]
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- 2009
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145. An ELIME-array for detection of aflatoxin B1 in corn samples
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Piermarini, S., Volpe, G., Micheli, L., Moscone, D., and Palleschi, G.
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AFLATOXINS , *CORN as food , *ENZYME-linked immunosorbent assay , *FOOD supply , *ELECTRODES , *TRANSDUCERS , *ACETONITRILE , *COOKING - Abstract
Abstract: The aim of the present work was the development of an ELIME-array to achieve simple and rapid detection of AFB1 in corn samples. The system is based on an indirect competitive ELISA format using magnetic beads as immobilisation support and eight magnetised screen-printed electrodes as electrochemical transducers. After an optimisation study, a corn sample treatment, employing an extraction in acetonitrile/water followed by a clean-up step and solvent evaporation, was selected. For the construction of the calibration curve, which was used to evaluate both evaluation of the matrix effect on the performances of the ELIME-array and for the analysis of Certified Reference Materials (CRMs), standard solutions of AFB1 were added to blank dried corn extracts reconstituted in PBS. The detection limit and the sensitivity of the assay were calculated to be 0.6ngmL−1 and 1.5ngmL−1, respectively. Precision (11–26%) and recovery (95–114%) data of the ELIME-array, determined by analysing four CRMs, have shown that the proposed system appears suitable as a screening tool for the analysis of AFB1 in corn samples. [Copyright &y& Elsevier]
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- 2009
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146. Electrochemical immunosensor array using a 96-well screen-printed microplate for aflatoxin B1 detection
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Piermarini, S., Micheli, L., Ammida, N.H.S., Palleschi, G., and Moscone, D.
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ELECTROCHEMICAL sensors , *AFLATOXINS , *ENZYME-linked immunosorbent assay , *MICROPLATES - Abstract
Abstract: A novel analytical immunosensor array, based on a microtiter plate coupled to a multichannel electrochemical detection (MED) system using the intermittent pulse amperometry (IPA) technique, is proposed for the detection of aflatoxin B1 (AFB1). In the present work, the electrochemical behaviour and electroanalytical performance of the thick-film carbon sensors (also designated as screen-printed electrodes) incorporated in the multichannel electrochemical plate were first evaluated. Then the 96-well screen-printed microplate was modified in accord with a competitive indirect enzyme-linked immunoassay (ELISA) format for aflatoxin B1 detection. The measurements were performed using both spectrophotometric and electrochemical procedures and the results of the calibration curves, detection limit (LOD), sensitivity and reproducibility of the respective assay systems were evaluated. The immunoassay was then applied for analysis of corn samples spiked with AFB1 before and after the extraction treatment, in order to study the extraction efficiency and the matrix effect, respectively. These studies have shown that using this system, AFB1 can be measured at a level of 30pg/mL and with a working range between 0.05 and 2ng/mL. Good recoveries (103±8%) were obtained, demonstrating the suitability of the proposed assay for accurate determination of the AFB1 concentration in corn samples. The specificity of the assay was assessed by studying the cross-reactivity of PAb relative to AFB1. The results indicated that the PAb could readily distinguish AFB1 from other aflatoxins, with the exception for AFG1. [Copyright &y& Elsevier]
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- 2007
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147. Acetylcholinesterase sensor based on screen-printed carbon electrode modified with prussian blue.
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Suprun, E., Evtugyn, G., Budnikov, H., Ricci, F., Moscone, D., and Palleschi, G.
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ACETYLCHOLINESTERASE , *BIOSENSORS , *CARBON electrodes , *PRUSSIAN blue , *PESTICIDES , *FOOD contamination - Abstract
Acetylcholinesterase (ChE) sensor based on Prussian blue (PB) modified electrode was developed and tested for the detection of organophosphorus and carbamic pesticides. The signal of the sensor was generated in PB mediated oxidation of thiocholine recorded at+200 mv in DC mode. ChE from electric eel was immobilized by cross-linking with glutaraldehyde in the presence of bovine serum albumin (BSA) on the surface of screen-printed carbon electrode covered with PB and Nafion. The content of the surface layer (specific enzyme activity, Nafion and BSA amounts) was optimized to establish high and reliable response toward the substrate and ChE inhibitors. The ChE/PB sensor makes it possible to detect Aldicarb, Paraoxon and Parathion-Methyl with limits of detection 30, 10 and 5 ppb, respectively (incubation 10 min). The feasibility of practical application of the ChE/PB sensor developed for the monitoring of degradation of the pesticides in wine fermentation was shown. To diminish matrix interferences, the electrolysis of the grape juice with Al anode and evaporation of ethanol were suggested, however the procedures decrease the sensitivity of pesticide detection and stability of the sample tested. [ABSTRACT FROM AUTHOR]
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- 2005
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148. Novel reagentless paper-based screen-printed electrochemical sensor to detect phosphate
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Daria Talarico, Fabiana Arduini, Danila Moscone, Stefano Cinti, Giuseppe Palleschi, Cinti, S, Talarico, D, Palleschi, G, Moscone, D, and Arduini, F
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Analyte ,paper-based electroanalytical platform ,Nanotechnology ,02 engineering and technology ,Standard solution ,reagentless ,01 natural sciences ,Biochemistry ,Analytical Chemistry ,screen-printed electrodes ,Environmental Chemistry ,Settore CHIM/01 - Chimica Analitica ,Spectroscopy ,phosphate ,wax-printing ,Detection limit ,Filter paper ,Chemistry ,010401 analytical chemistry ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Electrochemical gas sensor ,Linear range ,Reagent ,Electrode ,user-friendly method ,0210 nano-technology - Abstract
Herein we describe a novel reagentless paper-based electrochemical phosphate sensor, manufactured with a simple and inexpensive approach. By following three easy steps, consisting of wax patterning, paper chemical modification, and electrode screen-printing, the filter paper provides an effective electroanalytical platform to sense phosphate ions in standard solutions and real samples (river water). The electrochemical properties of the paper-based platform were evaluated, firstly, by using ferricyanide as a redox mediator, proving no analyte-entrapment due to the cellulose lattice. Then, the reference colorimetric method for phosphate ions, which is based on the formation of phosphomolybdic complex, was successfully adapted to a reagentless electrochemically paper-based platform. This novel and highly sustainable configuration readily allows for the determination of phosphate ions with high reproducibility and long storage stability, achieving a detection limit of 4 μM over a wide linear range up to 300 μM. This in-house approach would be able to generically develop an affordable in situ and user-friendly sensing device without the addition of any reagent, to be applied for a broad range of analytes.
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- 2016
149. Novel carbon black-cobalt phthalocyanine nanocomposite as sensing platform to detect organophosphorus pollutants at screen-printed electrode
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Daniela Neagu, Danila Moscone, Stefano Cinti, Fabiana Arduini, Ilaria Cacciotti, Marilena Carbone, Cinti, S, Neagu, D, Carbone, M, Cacciotti, I, Moscone, D, and Arduini, F
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Settore CHIM/03 - Chimica Generale e Inorganica ,Detection limit ,Nanocomposite ,Materials science ,General Chemical Engineering ,Biosensor Hybrid Nanocomposite Carbon Black Cobalt Phthalocyanine Organophosphorus pesticide ,010401 analytical chemistry ,Nanotechnology ,02 engineering and technology ,Carbon black ,021001 nanoscience & nanotechnology ,Electrochemistry ,01 natural sciences ,0104 chemical sciences ,Thiocholine ,Electrode ,Settore CHIM/01 - Chimica Analitica ,0210 nano-technology ,Dispersion (chemistry) ,Biosensor - Abstract
A facile one-step route to produce a homogenous and highly stable cobalt phthalocyanine (CoPc)-based dispersion by using carbon black (CB) as supporting material is reported. Herein, CB is proposed as effective material to load CoPc in order to obtain a CB/CoPc hybrid nanocomposite dispersion suitable for modifying screen-printed electrodes (SPEs) by an easy and automatable drop casting approach. CoPc resulted anchored to CB by a non-covalent physisorption, confirmed by IR and UV-visible spectroscopies, allowing to preserve the electrochemical performances of CoPc. The resulting CB/CoPc-modified SPE was tested as sensing tool to detect thiocholine, an enzymatic product of butyrylcholinesterase (BChE). The use of CB/CoPc leads to a highly sensitive thiocholine detection by applying a low potential (+0.05 V vs. internal reference) without fouling problem, a typical drawback that affects the thiol electrochemical detection. The favorable characteristics of the sensor were exploited for an easy BChE biosensor fabrication that renders this biosensor well suitable for mass-production. This electrochemical monoenzymatic biosensor was then challenged towards paraoxon, chosen as model organophosphorous pesticide, obtaining a low detection limit (18 nM). The suitability of the biosensor was tested in a waste water sample obtaining satisfactory recovery values, thus demonstrating its capability in such complex matrix.
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- 2016
150. Effective electrochemical sensor based on screen-printed electrodes modified with a carbon black-Au nanoparticles composite
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Fabiana Arduini, Renato Seeber, Giuseppe Palleschi, Chiara Zanardi, Stefano Cinti, Fabio Terzi, Danila Moscone, Arduini, F, Zanardi, C, Cinti, S, Terzi, F, Moscone, D, Palleschi, G, and Seeber, R
- Subjects
Materials science ,Analytical chemistry ,Screen-printed electrodes ,Carbon black ,Gold nanoparticles ,Nanocomposite ,Amperometric sensors ,Electrocatalysis ,Electrocatalyst ,chemistry.chemical_compound ,Screen-printed electrodes Carbon black Gold nanoparticles Nanocomposite Amperometric sensors Electrocatalysis ,Materials Chemistry ,Settore CHIM/01 - Chimica Analitica ,Electrical and Electronic Engineering ,Instrumentation ,Hydroquinone ,Metals and Alloys ,Condensed Matter Physics ,Ascorbic acid ,Amperometry ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Electrochemical gas sensor ,chemistry ,Chemical engineering ,Electrode - Abstract
A screen-printed electrode (SPE) modified with a carbon black (CB)-Au nanoparticles (AuNPs) composite is assembled and tested. Electrochemical and morphological investigations highlight the physico-chemical properties of the resulting AuNP-CB-SPE amperometric device with respect to SPEs modified with a single component of the nanocomposite. The effective performance of such a modified electrode in activating electrocatalytic processes, consisting both in oxidation and reduction reactions, is demonstrated. In particular, electrochemical tests on analytes such as glucose, hydrogen peroxide, hydroquinone, and ascorbic acid, evidence that the composite possesses electrocatalytic performance well superior with respect to the relevant mono-component modified SPE. As a consequence, a meaningful lowering of the peak potentials and improvement of the sensor sensitivities is observed when using AuNP-CB-SPEs with respect to both CB-SPEs and AuNP-SPEs. In the case of H2O2 reduction, the occurrence of the electrochemical process at less negative potentials is coupled to an improvement of sensor sensitivity of about one order of magnitude. Concurrently, lower limit of detections, ranging from 20 to 99% less, have been obtained for the major part of the analytes studied, i.e. glucose, hydrogen peroxide and hydroquinone. Preliminary results reported here indicate that AuNP-CB-SPE can be proposed as an efficient amperometric sensor to be used in many analytical applications.
- Published
- 2015
Catalog
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