Search

To improve the grinding of cassiteritepolymetallic sulfide ores, this paper studied the effect of six kinds of grinding aids. We found that these six kinds of grinding aids can change the grinding effect, but only PAM can make the eligible size fraction increase and decrease the lack-grinding size fraction and the overgrinding size fraction, and reaches the effect of the original study expectation.

Owing to fuzzy detail and distortion of underwater image and complex changes of target, the underwater target tracking system requires accuracy and continuity of tracking, and expects that the size of tracking window can adapt to appearance change of target. According to the requirements mentioned above, the underwater target tracking algorithm based on an improved color matching is proposed, which finds the best location of target through tracking accuracy algorithm and calculates width of window on the basis of tracking window size variation algorithm. The experimental results show that this algorithm can adaptively track the real-time target and has higher accuracy than traditional color matching algorithm.

The high-quality MCM-41 was synthesized from silica fume with Citric acid and cetyltrimethylammonium bromide (CTAB) as template agents. Silica fume entered into liquid system in silicate and matched with CTA+. Mesoporous phase formed with the help of Citric and PEG-6000 in an acidic process followed. The structure and morphology of mesoporous silica material so-prepared was investigated based on the analysis of X-ray diffraction (XRD), N2 sorption/desorption (BET) and transmission electron microscopy (TEM). The results indicated that MCM-41 was successfully prepared by substituting silica fume for the traditional silica source of tetraethyl orthosilicate, and the MCM-41 displayed high quality with the pore sizes 4.3 nm, the BET areas 1058 m2/g, the total pore volume 0.862 cm3/g.

The active-passive hybrid piezoelectric network (APPN) is investigated to reduce the vibration of cantilever beam. Hamilton’s principle with the Rayleigh-Ritz method is used to derive the equations of motion of the beam with the APPN. Only one piezoelectric actuator is bonded on the cantilever beam, so in the segment of the beam where the piezoelectric actuator is attached, the neutral axis is not the geometric center of the beam. This change on the neutral axis is considered in the process of deriving equations. Selecting RL circuit as passive shunt circuit, open-loop analysis is performed to gain insight into the passive damping features. Velocity feedback control is then employed to analyze the characteristics of the closed-loop system. Numerical results show that the APPN has a significant effect on vibration suppression, especially at narrow frequency bands. On this basis, variable RL circuit is proposed and analyzed for broadband vibration attenuation. Numerical simulations illustrate that this scheme is effective and feasible.

The technology of in situ air sparging (IAS) was used to remediate the groundwater polluted by petroleum hydrocarbon in an oil field. The results show that the soil geological condition has great influence on the injection air pathways distribution, and the pattern of the air pathways distribution is nonsymmetrical to the injection well as the vertical axis. The petroleum hydrocarbon removal rate reached to 70% in the zone with higher density of air pathways distribution, but only 40% in the zone with lower density of air pathways distribution, and the average removal rates of the petroleum hydrocarbon components reached to 60 % in the influence zone. The petroleum components with higher volatility are easily removed by volatilization, but those with lower volatility are difficult to remove, so a tailing effect of lingering residual contaminant exist when the technology is used to remediate groundwater polluted by petroleum hydrocarbon.

Main research object of this article is marine dredging silt in Tianjin Binhai region. It discusses natural settlement rule of dredged silt with different moisture content in Tianjin Binhai region through conducting indoor natural settlement model experiment to dredged silt of different moisture content. The results indicated that natural settlement of dredged silt in Tianjin Binhai region can be divided into three stages: the first stage is quick subsidence stage within 4-5 hours at the start of the experiment, during which settlement rate of dredged silt is fast and settlement volume is large; the second stage is natural deposition transition section stage within 2 days after the experiment, when settlement rate of dredged silt starts slow down but settlement volume is obvious; the third stage is dead-weight consolidation settlement stage of slow settlement after 2 days since the start of the experiment, in which settlement rate of dredged silt is slow and settlement volume is less. Settlement of Dredged silt in Tianjin Binhai region is greatly influenced by its initial moisture content. The higher the initial moisture content is the faster initial settlement rate of dredged silt will be and so is the final settlement volume of dredged silt.

A device, was used to recover dust and rhenium from molybdenum concentrate roasting exhaust gas, was developed. The device combine the self-swash dust catcher and spherical tray tower technology: the self-swash dust catcher was used to recover dust and rhenium; spherical tray tower was used to further dust and rhenium recovery and its application to engineering in practice. This was the first successful such devices used to recover dust and rhenium integration from calcination exhaust of molybdenum concentrate which contain rhenium in production practice. Through production practice, proved that the device is feasible, with high efficiency. And it has the advantages of simple structure, convenient operation, low cost and low energy consumption etc.. In addition, the dust removing system can also be used to the wet dust collector in the filed of metallurgical , mining, chemical and building.

The flotation process of molybdenum ore from Liaoning in China with a new collector DMC is investigated. The results show that the collector DMC has stronger collecting power and better selectivity for molybdenite. Compared with traditional kerosene and xanthate flotation,this process using the new collector could have a good index with 60% sodium silicate. The closed-circuit test results are as follows:the molybdenum concentrate of 53.04% with the molybdenum recovery of 95.02% is obtained.

Abietane diterpenes and miltirone series have shown important activities and for medical purposes in order to achieve the total synthesis of 1-oxomiltrone 1 and miltirone 4, a versatile intermediate 6 was found. The compound 6 could be used as a precursor A-B-C rings with different oxidative degrees in selected abietane diterpenes when synthesized through high yield Suzuki reaction and subsequent cyclization, and total synthesis of 1-oxomiltirone (1) has been achieved. Electronic Supplementary Material Supplementary material is available for this article at 10.1007/s13659-013-0034-7 and is accessible for authorized users.

In this paper, the feedback load reduction problem in wireless systems based on orthogonal frequency division multiplexing (OFDM) is investigated and an opportunistic feedback scheme (OFS) is proposed. The key idea behind OFS is that only the key channel gains which can significantly affect the system throughput are fed back to the BS. Firstly, the key channel gains are proved to belong to a channel gain interval. Secondly, a statistical method is proposed to estimate the channel gain interval. Thirdly, the opportunistic feedback scheme is formulated and the feedback load of OFS is analyzed. The advantage of OFS is threefold: the first is OFS can work in both OFDM-based multicast system and OFDM-based unicast system. The second is the channel fading type of the BS-user link is not required, which is more realistic. The third is OFS can get better feedback load performance compared with other schemes, while achieving almost the same throughput performance compared with that of full feedback scheme.

Four new labdane-type diterpenoid glycosides, laevissiosides A-D (1–4) were isolated from the 95% ethanol extract of Diplopterygium laevissimum (Christ) Nakai, along with two known analogues, 18- β-D-glucopyranosyl ester-sclareol (5) and 18-hydroxy-sclareol (6). The structures of compounds 1–4 were elucidated by extensive 1D and 2D NMR spectroscopy as well as high-resolution MS analyses. All isolated compounds were evaluated for their cytotoxic effects.

N(6)-methyl-adenosine (m(6)A) is one of the most common and abundant modifications on RNA molecules present in eukaryotes. However, the biological significance of m(6)A methylation remains largely unknown. Several independent lines of evidence suggest that the dynamic regulation of m(6)A may have a profound impact on gene expression regulation. The m(6)A modification is catalyzed by an unidentified methyltransferase complex containing at least one subunit methyltransferase like 3 (METTL3). m(6)A modification on messenger RNAs (mRNAs) mainly occurs in the exonic regions and 3'-untranslated region (3'-UTR) as revealed by high-throughput m(6)A-seq. One significant advance in m(6)A research is the recent discovery of the first two m(6)A RNA demethylases fat mass and obesity-associated (FTO) gene and ALKBH5, which catalyze m(6)A demethylation in an α-ketoglutarate (α-KG)- and Fe(2+)-dependent manner. Recent studies in model organisms demonstrate that METTL3, FTO and ALKBH5 play important roles in many biological processes, ranging from development and metabolism to fertility. Moreover, perturbation of activities of these enzymes leads to the disturbed expression of thousands of genes at the cellular level, implicating a regulatory role of m(6)A in RNA metabolism. Given the vital roles of DNA and histone methylations in epigenetic regulation of basic life processes in mammals, the dynamic and reversible chemical m(6)A modification on RNA may also serve as a novel epigenetic marker of profound biological significances.

Under the premise of comprehensive recycling valuable metals, a novel technology was developed to extract vanadium from spent Al2O3-based catalyst. Mixture of spent catalyst and sodium carbonate was roasted at 1000°C for 30 min with the mol ratio of Na2O to (Al2O3 + V2O5 + MoO3) as 1.15, after water leaching, 97.3% of vanadium could be extracted. With CaO addition of 25 g/L and reacting at 90°C for 4 h, 98.6% of vanadium in sodium aluminate solution was precipitated as desilication residue. The desilication residue was leached at 80°C for 45 min with sodium bicarbonate concentration of 100 g/L and L/S of 4 mL/g, over 96.4% of vanadium could be leached. By two purification steps with addition of 30 wt.% H2SO4 and Mg(NO3)2, respectively, over 99% of Al, 96% of Si, 93% of P and 95% of As were removed from leach liquor. Adding 50 g/L NH4NO3 to the purified leach liquor and adjusting pH to 8.2, 99.8% of vanadium could be precipitated as ammonium metavanadate. After calcination, the purity of V2O5 product was 98.25%. In the whole process, up to 88.7% of vanadium could be recovered from the spent catalyst.

Anemone flaccidaFr. Schmidt, a family of ancient hopanoids, have been used as traditional Asian herbs for the treatments of inflammation and convulsant diseases. Previous study on HeLa cells suggested that triterpenoid saponins fromAnemone flaccidaFr. Schmidt may have potential antitumor effect due to their apoptotic activities. Here, we confirmed the apoptotic activities of the following five triterpenoid saponins: glycoside St-I4a (1), glycoside St-J (2), anhuienoside E (3), hedera saponin B (4), and flaccidoside II (5) on human BEL-7402 and HepG2 hepatoma cell lines, as well as the model of HeLa cells treated with lipopolysaccharide (LPS). We found that COX-2/PGE2 signaling pathway, which plays key roles in the development of cancer, is involved in the antitumor activities of these saponins. These data provide the evidence that triterpenoid saponins can induce apoptosis via COX-2/PGE2 pathway, implying a preventive role of saponins fromAnemone flaccidain tumor.

In this study, we used microarray analysis to investigate the biogenesis and progression of intervertebral disc degeneration. The gene expression profiles of 37 disc tissue samples obtained from patients with herniated discs and degenerative disc disease collected by the National Cancer Institute Cooperative Tissue Network were analyzed. Differentially expressed genes between more and less degenerated discs were identified by significant analysis of microarray. A total of 555 genes were significantly overexpressed in more degenerated discs with a false discovery rate of < 3%. Functional annotation showed that these genes were significantly associated with membrane-bound vesicles, calcium ion binding and extracellular matrix. Protein-protein interaction analysis showed that these genes, including previously reported genes such as fibronectin, COL2A1 and β-catenin, may play key roles in disc degeneration. Unsupervised clustering indicated that the widely used morphology-based Thompson grading system was only marginally associated with the molecular classification of intervertebral disc degeneration. These findings indicate that detailed, systematic gene analysis may be a useful way of studying the biology of intervertebral disc degeneration.

Total synthesis of (±)-Lingzhilactone B has been achieved for the first time in 13 steps. The key step features a semipinacol rearrangement to construct 5/5 bicyclic ring with adjacent quaternary carbon.

Currently the research of face recognition mostly aim on little internal face of simple environment and carried out on single computer, which limited the application of face recognition. The emergence of big data technology make parallel compute more easy, which make big scale compute work could be accomplished in very short time. An dynamic face recognition system was put forward in this paper. The system was suited for comparing little internal face with numerous face which were collected by large amount of camera. Storm flow compute model was used in the face recognition which could get recognition result in real time and will send the result to the people who interested in. Experiment shows that storm could get face recognition result in one minute regardless the number of internal faces and collected faces. When the internal face increase or the collected face increase, we only increase the servers in the cluster, the recognition time could be limited in 1 minute. Experiment also shows that because big data technology use parallel compute technology, which make more complex recognition algorithm, more complex feature extract algorithm, higher resolution image, and more internal image, and thus improve the recognition accuracy. Because the above could be fulfilled under parallel compute technology, the result could be gotten in real time.

This paper is focus on the massive face recognition. We want to recognize a face from a lot of faces at public location. There are two kinds of techniques are used to hit the goal: one is the 3D face detection technique and the other is the Hadoop technique. Face detection technique find a similar face by 3D face features form mass face data. The Hadoop is a parallel processing structure, it can enhance the computation ability. From simulation results, it is demonstrate that our algorithm is an effective and correct method for massive face recognition.

Enantioselective total synthesis of (+)-Lingzhiol has been achieved. It is the first example of in tandem semipinacol rearrangement reactions, the migrated aryl group further reacting with the carbonyl oxonium electrophile to furnish a polycyclic skeleton. Our synthesis involves 13 steps and proceeds in 6% overall yield.

Osteoarthritis (OA) has a profound impact on health-related quality of life 1 . Increasing importance has been attached to utilization of disease-specific, self-reported outcome measures 2 , such as the Hip Disability and Osteoarthritis Outcome Score (HOOS) instrument 3 . China is the most populous country in the world with 1.3 billion people. Hence, we translated and adapted the HOOS into a Simplified Chinese version (SC-HOOS) and validated it in a cohort of native Chinese-speaking patients with hip OA, relative to the Short Form Health Survey (SF-36), a visual analog scale (VAS), and the Harris hip score (HHS) test. Psychometric testing for internal consistency, testeretest reliability, construct validity, and responsiveness was conducted. The SC-HOOS showed satisfactory internal consistency, testeretest reliability, construct validity, and responsiveness when evaluated in Chinese-speaking patients with hip OA.

The special structure of beams of cantilever screen has the advantages comparing with the traditional screen and special dynamic characteristics, such as the dynamic characteristics of frequency veering. The traditional analysis method of vibrating screen is not applicable to its dynamic analysis because of the special structure. By analysis and decomposition of stiffness matrix of beams of cantilever screen, the frequency veering phenomenon of the beam of cantilever screen was explained from the point of view of modal energy equation and the influence of sub-stiffness matrix of beams of cantilever screen on the frequency veering was analyzed. The analysis results showed that the sub-stiffness matrix of the hollow beam and sub-stiffness matrix of cantilever bars is the main reason for the segmentation of modal natural frequencies and the coupling stiffness between hollow beam and cantilever bars is the main reason for the concentration of modal natural frequencies and the formation of the regular mode shapes for cantilever bars group.

The electronic structures and magnetic properties of Pd-doped CdS have been studied by FLAPW method in the GGA. We find that the Pd-doped CdS becomes 100% spin polarized at fermi level when Pd substitutes for Cd atom both in 3×3×2 and 2×2×2 CdS supercells. The supercell magnetic moment reaches to 2.0μB. At the same time, the magnetic coupling interaction makes the occupied states more localized. The magnetism, the density of states, the spin density and band structure were studied as well.

Integration of the genetic and metabolic fingerprinting can provide a new approach to differentiate similar Traditional Chinese Medical (TCM) materials. Two leguminous plants, Mojia Huangqi and Menggu Huangqi, are important medical herbs and share great similarities in morphology, chemical constituent, and genomic DNA sequence. The taxonomy of Mojia Huangqi and Menggu Huangqi has been debated for more than 50 years and discrimination of TCM materials directly affects the pharmacological and clinical effects. AFLP based genetic fingerprinting and GC-TOF/MS-based metabolic fingerprinting were used to successfully discriminate the two species. The results of AFLP supported the opinion that Menggu Huangqi was a variant of Mojia Huangqi. The metabolic fingerprinting showed growth locations have greater impacts on the metabolite composition and quantity than the genotypes (cultivated versus wild) in Menggu Huangqi. The difference of some soluble sugars, fatty acids, proline, and polyamine reflected plant adaptation to different growth environments. Using multivariate and univariate statistical analysis, three AFLP markers and eight metabolites were identified as candidate DNA and metabolic markers to distinguish the two herb materials. The correlation network between AFLP markers and metabolites revealed a complex correlation network, which indicated the special metabolic pathways and the regulation networks of Huangqi.

In this study, the mesoporous silica material was hydrothermally synthesized from the rice husk ash of biomass energy industry with cetyltrimethylammonium bromide (CTAB) as template agents. The structure and morphology of mesoporous silica material was investigated based on the analysis of X-ray diffraction (XRD), N2 sorption/desorption (BET), Fourier transformation infra-red spectrum (FTIR). The results indicated that mesoporous silica material was successfully prepared by substituting rice husk ash for the traditional silica source of tetraethyl orthosilicate, and the mesoporous silica material displayed pore sizes in the 2-4 nm range with specific surface areas as high as 363 m2/g.

A novel and efficient trifluoromethanesulfonic acid-catalyzed sp(3)-sp(2) C-C bond formation reaction through the direct coupling of alcohols with alkenes has been realized under mild conditions. The present protocol provides an attractive approach to a diverse range of polysubstituted olefins in good to excellent yields with high stereo- and regioselectivities.

Based on the two-dimensional Direct Linear Transformation (DLT) principle of close-range digital photogrammetry and mathematical principle of the linear neuron, the equivalent relationship between linear neural network and the two-dimensional DLT of close-range digital photogrammetry is discussed. A neural network with 2 linear neurons, 6 input parameters and 2 output parameters is established to simulate the two-dimensional DLT. The network can be trained using a set of grid points in the control coordinate system with known world coordinates and pixel coordinates. The weights and biases of trained network contain camera interior and exterior parameters. A new digital photographic technique is put forward combined camera self-calibration based on neural network with non-linear pixel coordinates correction of lens distortion. The indoor survey test indicates that measurement is more accuracy. Meanwhile, the new technology is successfully used in crack monitor of a bridge pier.

In order to realize scientific planning the layout of strategic loading station, the strategic-loading –station location problem was proposed, which is related to determine the location of each stay with station and the transportation plan on the precise of satisfying the general location constraints and problem related special constraints. Based on theory of capacitated facility location with multiple souring in two stage supply chain, one mixed integer linear programming model was established which took whole logistic cost embedding transportation and location costs as objective. When confronted with small and medium size problem, this model could be exactly solved by optimization software using branch and bound algorithm, and when the problem size was big, this model could be heuristically solved by genetic algorithm which used 0-1 and priority-based combined encoding method to represent solution, cost-based and random-generated combined method to generate initial solution, solution repaired strategy and penalty method and special fitness function to deal illegal individual, and greedy based method to heuristically solve transshipment problem. At last, according to computational tests on randomly generated data demonstrated the practical feasibility of this method.

Four new acylphloroglucinols with an unusual 6/6/5 spirocyclic skeleton, hyperbeanols A-D (1-4), were isolated from the methanol extract of Hypericum beanii along with 16 known compounds. Their structures were established on the basis of spectroscopic and X-ray diffraction analysis. Hyperbeanols A-C were three stereoisomers different only at the relative configuration of C-4 and C-13, which were distinguished by the nuclear Oyerhauser effect spectroscopy (NOESY) spectroscopic data in combination with the single X-ray analysis of hyperbeanol A (1). The cytotoxic activity of hyperbeanols A D against the cancer cell lines SK-BR-3, HL-60, SMMC-7721, PANC-1, MCF-7, and K562 was also evaluated.

Catalytic asymmetric formal total synthesis of (+)-dichroanone and (+)-taiwaniaquinone H has been achieved. Key step involved construction of all-carbon quaternary carbon by palladium-catalyzed conjugate addition of arylboronic acid to 3-methyl cyclohexenone. Furthermore, a new approach to build [6-5-6] tricyclic backbone via formyl introduction and subsequent aldol-type condensation was also explored. (C) 2014 Elsevier Ltd. All rights reserved.

An unusual oxindole alkaloid inner salt, macrophyllionium (1), and a pair of new tetracyclic oxindole alkaloids, macrophyllines A (2) and B (3), together with six known alkaloids, were isolated from the aerial parts of Uncaria macrophylla. Corynantheidine (8) exhibited moderate cytotoxicity against HL-60 and SW480 cells with IC(50) values of 13.96 and 23.28 mu M, respectively. Dihydrocorynantheine (9) exhibited significant vasodilating activity against phenylephrine-induced contraction in rat thoracic aorta rings (IC(50) = 6.73 mu g/mL). In addition, compounds 2, 6, and 9 showed weak inhibitory action on KO-induced contraction.

In an attempt to find new types of anti-hypoxic agents from herbs, we identified 5-hydroxymethyl-2-furfural (5-HMF) as a natural agent that fulfills the criterion. 5-HMF, the final product of carbohydrate metabolism, has favorable biological effects such as anti-oxidant activity and inhibiting sickling of red blood cells. The role of 5-HMF in hypoxia, however, is not yet. Our pilot results showed that pretreatment with 5-HMF markedly increased both the survival time and the survival rate of mice under hypoxic stress. The present study was aimed to further investigate the protective role of 5-HMF and the underlying mechanisms in hypoxic injury using ECV304 cells as an in vitro model. ECV304 cells pretreated with or without 5-HMF for 1 h were exposed to hypoxic condition (0.3% O(2)) for 24 h and then cell apoptosis, necrosis, the changes of mitochondrial membrane potential (MMP) and the expressions of phosphorylation- extracellular signal-regulated kinase (p-ERK) were investigated. Pretreatment with 5-HMF markedly attenuated hypoxia-induced cell necrosis and apoptosis at late stage (p 0.01). Furthermore, pretreatment with 5-HMF rescued both the decline of the MMP and the increase of p-ERK protein under hypoxia. In a word, these results indicated that 5-HMF had protective effects against hypoxic injury in ECV304 cells, and its effects on MMP and p-ERK may be involved in the mechanism.

Two new C(30)-epimeric polycyclic polyprenylated acylphloroglucinols (PPAPs), named uralodins B and C (1 and 2, resp.), were isolated from the aerial parts of Hypericum henryi subsp. uraloides together with two new chromone glucosides, urachromones A and B (3 and 4, resp.), as well as 16 known compounds. Their structures were established by extensive NMR techniques and MS analysis. The epimers 1 and 2 always behaved like a single compound when examined by TLC, and were separated by HPLC. Their configuration was distinguished by comparative analysis of the NMR data with known analogues together with the ROESY experiment. All the isolated PPAPs were evaluated for their cytotoxic activities against HepG2, SGC7901, HL-60, and K562 cell lines. Compound 1 showed modest cytotoxic activities against SGC7901 and HL-60 cell lines, and 2 showed modest cytotoxic activities against HepG2, SGC7901, HL-60, and K562 cell lines.

Phytochemical investigation on the methanol extract of Brainea insignis led to the isolation of 16 phenolic compounds, including an unusual flavanol coupled with phenylpropyl and shikimic acid units, brainicin (1), and a new flavonol acylglycosides brainoside B (2). The structures of new compounds were elucidated on the basis of extensive spectroscopic analysis. Compounds 1-5 were evaluated for their cytotoxic effects. However, none of the compounds showed significant cytotoxic activity against the HL-60, A549, SMMC-7721, MCF-7, SW480 cell lines (IC(50)40 microM).

It has been demonstrated that hypoxic preconditioning (HP) enhances the survival ability of the organism against the subsequent acute anoxia (AA). However, it is not yet clear whether necrosis induced by AA can be prevented by HP, and what are the underlying mechanisms. In this study, we examined the effect of HP (10% O(2), 48 h) on necrosis induced by AA (0% O(2), 24 h) in PC12 cells. We found that HP delayed the regulatory volume decrease and reduced cell swelling after 24 h of exposure to AA. Since aldose reductase (AR) is involved in cell volume regulation, we detected AR mRNA expression with reverse transcription-polymerase chain reaction (RT-PCR) techniques. The AR mRNA level was dramatically elevated by HP. Furthermore, an HP-induced decrease in cell injury was reversed by berberine chloride (BB), the inhibitor of AR. In addition, sorbitol synthesized from glucose catalyzed by AR is directly related to cell volume regulation. Subsequently, we tested sorbitol content in the cytoplasm. HP clearly elevated sorbitol content, while BB inhibited the elevation induced by HP. Further study showed that a strong inhibitor of sorbitol permease, quinidine, completely reversed the protection induced by HP after AA. These data provide evidence that HP prevents necrosis induced by AA and is mediated by AR and sorbitol pathway.

Formalin-fixed paraffin-embedded (FFPE) tissue specimens comprise a potentially valuable resource for retrospective biomarker discovery studies, and recent work indicates the feasibility of using shotgun proteomics to characterize FFPE tissue proteins. A critical question in the field is whether proteomes characterized in FFPE specimens are equivalent to proteomes in corresponding fresh or frozen tissue specimens. Here we compared shotgun proteomic analyses of frozen and FFPE specimens prepared from the same colon adenoma tissues. Following deparaffinization, rehydration, and tryptic digestion under mild conditions, FFPE specimens corresponding to 200 microg of protein yielded approximately 400 confident protein identifications in a one-dimensional reverse phase liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. The major difference between frozen and FFPE proteomes was a decrease in the proportions of lysine C-terminal to arginine C-terminal peptides observed, but these differences had little effect on the proteins identified. No covalent peptide modifications attributable to formaldehyde chemistry were detected by analyses of the MS/MS datasets, which suggests that undetected, cross-linked peptides comprise the major class of modifications in FFPE tissues. Fixation of tissue for up to 2 days in neutral buffered formalin did not adversely impact protein identifications. Analysis of archival colon adenoma FFPE specimens indicated equivalent numbers of MS/MS spectral counts and protein group identifications from specimens stored for 1, 3, 5, and 10 years. Combination of peptide isoelectric focusing-based separation with reverse phase LC-MS/MS identified 2554 protein groups in 600 ng of protein from frozen tissue and 2302 protein groups from FFPE tissue with at least two distinct peptide identifications per protein. Analysis of the combined frozen and FFPE data showed a 92% overlap in the protein groups identified. Comparison of gene ontology categories of identified proteins revealed no bias in protein identification based on subcellular localization. Although the status of posttranslational modifications was not examined in this study, archival samples displayed a modest increase in methionine oxidation, from approximately 17% after one year of storage to approximately 25% after 10 years. These data demonstrate the equivalence of proteome inventories obtained from FFPE and frozen tissue specimens and provide support for retrospective proteomic analysis of FFPE tissues for biomarker discovery.

A study of the effects of the pulp nature on desulphurization and de-ashing during high-sulfur coal flotation is described in this paper. Highlighted are the use of a solution oxygen gauge, a pH value gauge and a surface tension gauge to investigate changes in the pulp nature related to changes in the oxygen content, the pH value and the interfacial tension. The temperature before and after ultrasonic conditioning was also investigated. The results showed that ultrasonic conditioning resulted in a decrease in the oxygen content and the interfacial tension and an increase in the pH value and the temperature of the pulp. The perfect index of flotation and the perfect index of desulphurization of fine coal increased by 25.19% and 18.03%, respectively, after the pulp was ultrasonically conditioned. This study shows that ultrasonic conditioning can change the pulp nature and enhance the degree of desulphurization during high-sulfur coal flotation.

Concise synthesis of (±)-Lingzhiol has been achieved. The key reaction involves one-step construction of a 5/5/6/6 tetra-ring backbone of Lingzhiol via epoxy-arene cyclization.

A retrospective study. To summarize and describe the radiographic parameters of adult degenerative scoliosis (ADS) and explore the radiological parameters which are significantly different in sagittal balanced and imbalanced ADS patients. ADS is the most common type of adult spinal deformity. However, no comprehensive description of radiographic parameters in ADS patients has been made, and few studies have been performed to explore which radiological parameters are significantly different between sagittal balanced and imbalanced ADS patients. Medical records of ADS patients in our outpatient clinic from January 2012 to January 2014 were reviewed. Demographic data including age and sex, and radiographic data including the coronal Cobb angle, location of apical vertebra/disc, convexity of the curve, degree of apical vertebra rotation, curve segments, thoracic kyphosis (TK), lumbar lordosis (LL), thoracolumbar kyphosis (TL), sacral slope (SS), pelvic tilt (PT), pelvic incidence (PI), sagittal vertical axis (SVA), and PI minus LL (PI − LL) were reviewed to make comprehensive description of radiographic parameters of ADS. Furthermore, patients were divided into 2 groups according to whether the patients’ sagittal plane was balanced: Group A (imbalanced, SVA > 5 cm) and Group B (balanced, SVA ≤ 5 cm). Demographic and radiological parameters were compared between these 2 groups. A total of 99 patients were included in this study (Group A = 33 and Group B = 66; female = 83 and male = 16; sex ratio = 5:1). The median of age were 67 years (range: 41–92 years). The median of coronal Cobb angle and length of curve was 23 (range: 10–75°) and 5 segments (range: 3–7), respectively. The most common location of apical vertebra was at L2 to L3 (81%) and the median of degree of apical vertebra rotation was 2° (range: 1–3). Our study also showed significant correlations between coronal Cobb angle and curve segments (r = 0.23, P

Polycyclic polyprenylated acylphloroglucinols (PPAPs) are a class of hybrid natural products sharing the mevalonate/methylerythritol phosphate and polyketide biosynthetic pathways and showing considerable structural and bioactive diversity. In a systematic phytochemical investigation of Hypericum henryi, 40 PPAP-type derivatives, including the new compounds hyphenrones G-Q, were obtained. These compounds represent 12 different structural types, including four unusual skeletons exemplified by 5, 8, 10, and 17. The 12 different core structures found are explicable in terms of their biosynthetic origin. The structure of a known PPAP, perforatumone, was revised to hyphenrone A (5) by NMR spectroscopic and biomimetic synthesis methods. Several compounds exhibited inhibitory activities against acetylcholinesterase and human tumor cell lines. This study deals with the structural diversity, function, and biogenesis of natural PPAPs.

Five selective 11 beta-hydroxysteroid dehydrogenase type 1 (11 beta-HSD1) competitive inhibitors, hupehenols AE (1-5), were isolated from Viburnum hupehense. The structure elucidation indicated that compounds 1-5 are new 20,21,22,23,24,25,26,27-octanordammarane triterpenoids. Their structures were established on the basis of NMR spectroscopic and mass spectrometric analysis. Hupehenols AE (1-5) showed inhibition against human 11 beta-HSD1, with hupehenols B (2) and E (5) having IC50 values of 15.3 and 34.0 nM, respectively. Moreover, hupehenols C (3) and D (4) are highly selective inhibitors of human 11 beta-HSD1 when compared to murine 11 beta-HSD1.T

Inflammatory cytokine interleukin-17 (IL-17) has been associated with the risk of progressive cancers including lung cancer. However, it remains unclear how IL-17 may contribute to the invasion and development of these inflammation-associated malignancies. Here we aimed to investigate the role of IL-17 in lung cancer cell development. Epithelial-mesenchymal transition (EMT) has been recently proposed as a developmental process which plays an important role in cancer progression and metastases. Here we show that IL-17 might promote EMT in lung cancer cells by inducing the transcriptional repressor ZEB1. Exposure to IL-17 upregulated the signature EMT phenotypic markers vimentin and E-cadherin in lung cancer cells, and compared with controls, increased cell migration was observed in IL-17-treated lung cancer cells. ZEB1 mRNA and protein expression was induced by IL-17, and IL-17 stimulated nuclear localization of phosphorylated ZEB1. Conversely, suppressing ZEB1 expression by ZEB1 siRNA abrogated IL-17-stimulated vimentin expression and cell migration. Moreover, the phosphorylation of IκBα was required for IL-17-induced expression of ZEB1, suggesting the involvement of canonical NF-κB signaling. To check this hypothesis, we used IKK inhibitor BAY 11-7028 to block NF-κB activity. We found that BAY 11-7028 abrogated IL-17-induced ZEB1 expression, cell migration, and EMT, thus confirming that NF-κB is required for IL-17 to induce these aggressive phenotypes in lung cancer cells. Taken together, our data support the idea that IL-17-induced EMT promotes lung cancer cell migration and invasion via NF-κB-mediated upregulation of ZEB1. This study reveals a new signaling axis through which the tumor microenvironment causes ZEB1 expression to promote cancer metastasis. We suggest that targeting IL-17-induced ZEB1 expression may offer an effective therapeutic strategy for lung cancer treatment.

Sustav ventilacije u rudniku važna je sastavnica podzemnog sustava za vađenje rude. Poznato je da je projekt ventilacijskog sustava od velike važnosti u rudnicima s izvanredno velikim količinama plina te je model ventilacije primarni čimbenik u reguliranju plina kod potpuno mehaniziranih iskopa. Budući da je količina ispuštanja plina posebno velika u potpuno mehaniziranim iskopima s krajnje visokim količinama plina te da U model ventilacije ozbiljno ograničava normalnu proizvodnju, potreban je novi model ventilacije. U ovom je radu primijenjena metoda numeričke simulacije distribucije protoka plina u analizi učinka reguliranja plina u modelima U+L i Y+L. Rezultati pokazuju da je koncentracija plina u U+L modelu ventilacije niža nego u modelu Y+L kod potpuno mehaniziranog iskopa i njegovog gornjeg ugla. Nadalje, glavni čimbenici ventilacije koji utječu na odvod plina kod U+L modela ventilacije optimizirani su ortogonalnim testom i metodom fuzzy evaluacije; tako se učinkovito smanjila koncentracija plina u gornjem uglu i povratnom prolazu za ventilaciju. Nakon implementacije optimalnog rješenja modela ventilacije U+L u iskopu No. 32212(1) rudnika ugljena Rujigou u autonomnoj pokrajini Ningxia Hui u Kini, koncentracija plina u gornjem uglu smanjena je na 0,6 %, što može osigurati normalnu proizvodnju ugljenokopa., A mine ventilation system is an important component of an underground mining system. We all know that the ventilation system design plays an important role in highly gassy mines, and the ventilation pattern is the primary factor in gob gas control in fully mechanized working faces. Because the gas emission rate is notably large in highly gassy fully mechanized working faces and the U ventilation pattern seriously restricts normal production, a new ventilation pattern is required. This paper uses a gas flow distribution numerical simulation method to analyze the gas controlling effect of U+L and Y+L patterns. The results indicate that the gas concentration of the U+L ventilation pattern is lower than that of Y+L pattern in a fully mechanized working face and its upper corner. Furthermore, the main ventilation factors that affect the gas drainage of U+L ventilation pattern are optimized through the orthogonal test and fuzzy evaluation method; it effectively reduces the gas concentration of the upper corner and the return airway. After the implementation of the optimal solution of the U+L ventilation pattern in No. 32212(1) working face of Rujigou coal mine of Ningxia Hui Autonomous Region in China, the gas concentration of the upper corner was reduced to 0,6 %, which can ensure the normal production of the coal mine.