101. In Vivo Ear Sponge Lymphangiogenesis Assay.
- Author
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Akwii RG, Sajib MS, Zahra FT, Madala HR, Srivenugopal KS, and Mikelis CM
- Subjects
- Angiopoietin-2 genetics, Animals, Ear surgery, Humans, Immunity immunology, Immunity physiology, Lymphangiogenesis genetics, Lymphangiogenesis immunology, Lymphatic Vessels immunology, Mice, Signal Transduction genetics, Vascular Remodeling genetics, Vascular Remodeling immunology, Vascular Remodeling physiology, Wound Healing genetics, Wound Healing physiology, Biological Assay methods, Ear physiopathology, Lymphangiogenesis physiology, Lymphatic Vessels physiology
- Abstract
Lymphangiogenesis, the formation of lymphatic vessels from preexisting ones, is an important process in wound-healing physiology. Deregulation of lymphangiogenesis and lymphatic vascular remodeling have been implicated in a range of inflammatory conditions, such as lymphedema, lymphadenopathy, tumor growth, and cancer metastasis. Any attempt in understanding various parameters of the lymphangiogenic process and developing desirable therapeutic targets requires recapitulating these conditions in in vivo models. One pitfall with some experimental models is the absence of immune response, an important regulatory factor for lymphangiogenesis. We overcome this issue by using immune competent mice. In this chapter, by using Angiopoietin-2 (Ang2), a protein that belongs to the Ang/Tie signaling pathway, we describe the ear sponge assay with important adaptations, highlighting a reproducible and quantitative tool for assessment of in vivo lymphangiogenesis.
- Published
- 2021
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