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Community associated methicillin resistant Staphylococcus aureus (CA-MRSA) is an emerging pathogen increasingly reported to cause skin and soft tissue infections for children. The emergence of highly virulencet CA-MRSA strains in the immunodeficiency of young children seemed to be the basic explanation of the increased incidence of CA-MRSA infections among this population. The subjects of this study were 8 patients hospitalized in the Pediatric Department at the University Hospital of Monastir. The patients were young children (aged from 12 days to 18 months) who were suffering from MRSA skin infections; two of them had the infections within 72 h of their admission. The isolates were classified as community isolates as they all carried the staphylococcal cassette chromosome mec (SCCmec) IV and pvl genes. Epidemiological techniques, pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST), were applied to investigate CA-MRSA strains. Analysis of molecular data revealed that MRSA strains were related according to PFGE patterns and they belonged to a single clone ST80. Antimicrobial susceptibility tests showed that all strains were resistant to kanamycin and 2 strains were resistant to erythromycin. [ABSTRACT FROM AUTHOR]

The main purpose of this paper is to develop a new manufacturing process leading to have antibacterial dyed non-absorbable braided polyethylene terephthalate (PET) sutures using biocompatible and non-toxic products. This manufacturing process allows better visibility of sutures in the surgical field and reduces the risk of infections and inflammatory reactions without affecting the mechanical properties while meeting the United States Pharmacopeia (USP) requirements. Plasma functionalization, acrylic acid (AA) grafting, and bioactive chitosan (CH) coating were used before the dyeing process with a biocompatible non-toxic acid dye, approved by the Food and Drug Administration (FDA). The influence of experimental parameters on the suture properties and the K/S values of the dyed sutures are investigated. Infrared spectroscopy confirms the presence of new bonds to immobilize chitosan on the surface of the suture. Mechanical tests confirm that the mechanical properties of sutures have not been affected. The in vitro antibacterial effect of dyed PET sutures showed an inhibition zone of 11 mm against S. aureus, 4 mm against P. aeruginosa, and 1 mm against E. coli. This study reveals that the new finishing process of sutures is a promising method to achieve an antibacterial effect with a uniform shade and smooth surfaces. [ABSTRACT FROM AUTHOR]

Lben is a dairy fermented food that is largely consumed in Tunisia for its numerous health benefits that are related to the existence of probiotics. Lactic Acid Bacteria (LAB) are well known for their beneficial probiotic properties for humans, especially when administered in adequate amounts. The aim of this study was to isolate and investigate the probiotics properties of Lacticaseibacillus paracasei L2 from Lben. The isolated strain was identified by 16S r-RNA gene sequences and MALDI- TOF MS. To evaluate the probiotic potential of the isolated bacterium, in vitro tests were performed, including adhesion ability to HCT-116 cells, survival in acid and bile salt conditions, lysozyme resistance, biofilm formation, hemolytic activity, antioxidant activity, and antimicrobial activity. Our results revealed that the selected Lacticaseibacillus paracasei L2 strain expressed a high adherence to HCT-116 cells (45.03%), survived under acidic conditions (pH3), and showed a resistance to bile salts. The strain was considered as safe (α-hemolysis). L. paracasei L2 showed a high biofilm-formation ability (OD 570 > 1.7) after 24 h of incubation. It also demonstrated an important antioxidant activity in the range of 85.31% for the intact cells. However, an antimicrobial activity against pathogens, namely Staphylococcus aureus, was detected with an IZ that was above 19 mm. In conjunction with the results obtained and the technological properties of Lacticaseibacillus paracasei L2 (proteolytic property, autolytic activity, acidifying activity, and EPS production), this strain may be used as a probiotic for manufacturing fermented foods.

Background: Laurus nobilis L. is an aromatic herb with relevant medicinal properties due to its important chemical composition and its potential therapeutic effects. In this study, we investigate the chemical composition, the antibacterial and the antibiofilms activities of Tunisian L. nobilis L. essential oils against clinical Staphylococcus aureus strains., Methods: The chemical composition of L. nobilis L. essential oils was analysed by Gas Chromatography-Mass Spectrometry (GC-MS). The antibacterial activity of L. nobilis L. essential oils was evaluated in vitro against oral S. aureus (n=21) strains using broth microdilution method. The antibiofilm activity was assessed via Crystal Violet staining and MTT assays., Results: Our results revealed that GC-MS assay exhibited 1.8-Cineole, methyl eugenol and α-terpinyl acetate as the major compounds in the essential oils. Moreover, the essential oil from Sousse exhibited the best bactericidal activity (MICs values ranged from 3.91 to 15.62mgm -1 ). Furthermore, this oil showed a strong biofilm inhibition effect above 70%, from a low sub-inhibitory concentration (1/16×MIC). MTT assay revealed that both essential oils displayed an excellent antibiofilm activity with eradication percentages ranging from 79.6±2.27 to 95.2±0.56., Conclusion: Our finding demonstrated that L. nobilis L. essential oils are able to inhibit oral S. aureus strains with important antibiofilm efficacy. It could have a promising role in the prevention of oral diseases., (Copyright © 2015 Elsevier Masson SAS. All rights reserved.)

Community associated methicillin resistant Staphylococcus aureus (CA-MRSA) is an emerging pathogen increasingly reported to cause skin and soft tissue infections for children. The emergence of highly virulencet CA-MRSA strains in the immunodeficiency of young children seemed to be the basic explanation of the increased incidence of CA-MRSA infections among this population. The subjects of this study were 8 patients hospitalized in the Pediatric Department at the University Hospital of Monastir. The patients were young children (aged from 12 days to 18 months) who were suffering from MRSA skin infections; two of them had the infections within 72 h of their admission. The isolates were classified as community isolates as they all carried the staphylococcal cassette chromosome mec (SCCmec) IV and pvl genes. Epidemiological techniques, pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST), were applied to investigate CA-MRSA strains. Analysis of molecular data revealed that MRSA strains were related according to PFGE patterns and they belonged to a single clone ST80. Antimicrobial susceptibility tests showed that all strains were resistant to kanamycin and 2 strains were resistant to erythromycin.

Bronchial foreign body inhalation is a frequent and potentially serious accident in children. It can provoke long-term complications such as bronchiectasis and recurrent pulmonary infections. The foreign body is generally blocked in a bronchus and induces obstructive emphysema with superinfection. We report 2 cases of bronchial inhalation of a migrating cereal-ear, eliminated by parietal fistulization, in 9- and 11-year-old boys.