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The aim of this study was to investigate whether a genetic combined effect exists between CD40-1CT and CTLA-4+6230GA (CT60) polymorphisms and whether the combined effect renders susceptibility to Graves' disease (GD). We recruited 260 patients with GD and 248 healthy controls. Single nucleotide polymorphisms were genotyped by polymerase chain reaction-high resolution melting. Genetic polymorphisms related to GD were identified, levels of thyroid stimulating hormone receptor antibodies (TRAb) were measured, and genetic interactions were assessed by logistic regression analysis. Significant difference in allele and genotype frequency of CD40-1CT polymorphism was observed between the patients and control subjects (P0.001, 0.002 respectively). As for CTLA-4+6230GA polymorphism, significant difference was observed only in allele frequencies between the patient and control groups (P=0.014). Moreover, a significant combined effect was presented in CD40-1CT and CTLA-4+6230GA polymorphism (P=0.020), and all, but one, combination CC-genotype of CD40-1CT and GG-genotype of CTLA-4+6230GA polymorphism has 54% lower risk of GD development than subjects with the CC and GG genotypes (OR=0.46, 95% CI=0.25-0.84). In newly onset GD group, neither single SNP (CD40-1CT or CTLA-4+6230GA polymorphism) nor their combined effect was showed a significant association with TRAb concentration (all P0.05). Our findings suggest a possible additive combined effect between CD40-1CT and CTLA4+6230GA polymorphisms in the development of GD.

6063 Background: A prospective, randomized, and multicentric phase II study was performed to evaluate the short-term efficacy and safety of Endostar plus intensity-modulated radiotherapy (IMRT) versus concurrent chemoradiotherapy(CCRT) in locally advanced low-risk nasopharyngeal carcinoma(NPC). Methods: From September 2014 to August 2016, 120 patients with low-risk NPC at stages III-IVa from 9 centers were randomly divided into experimental group (Endostar plus radiotherapy (ERT); n = 60) and control group (CCRT; n = 60). ERT patients were given Endostar (7.5 mg/m2/day) by continuous intravenous infusion (CIV) from 5 days before radiotherapy for consecutive 10 days for 2 cycles with an interval of 14 days. Then, ERT patients received 2 cycles of 10 days of maintenance treatment with Endostar after radiotherapy. The CCRT patients were given cisplatin (100 mg/m2) on days 1, 22, and 43 for 3 cycles. Immediate and 3-month efficacy and adverse effects were evaluated between the two groups. ClinicalTrials registration number was NCT02237924. Results: All patients were eligible for toxicity and response analysis. Regarding immediate efficacy, the complete response(CR) rates were 45.0% for ERT arm and 33.3% for CCRT arm in nasopharynx (P = 0.190), and 43.3% for ERT arm and 36.7% for CCRT arm in regional nodes (P = 0.456). Three months after RT, the CR rates were 71.2% for ERT arm and 60.0% for CCRT arm in nasopharynx (P = 0.151), and 74.6% for ERT arm and 63.3% for CCRT arm in regional nodes (P = 0.172). The rate and severity of leukopenia, hemoglobin reduction and thrombocytopenia in ERT arm were significantly lower than CCRT arm (P < 0.01). The occurrence rates of Xerostomia, oral mucositis, nausea / vomiting, constipation and weight loss in ERT arm were significantly lower than those in CCRT arm (P < 0.01). Conclusions: The present study demonstrates that ERT has similar short-term efficacy on locally advanced low-risk NPC compared with CCRT, but the acute adverse effects of ERT are fewer, and the compliance and tolerability of patients are better. Clinical trial information: NCT02237924.

TH17 cells play an essential role in the development of both human multiple sclerosis and animal experimental autoimmune encephalomyelitis (EAE). Nevertheless, it is not well understood how the pathogenicity of TH17 cells is controlled in the autoimmune neuro-inflammation. In vitro, we found Lumican (Lum), an extracellular matrix (ECM) protein, is selectively expressed by TH17 cells among tested murine TH subsets. Lum-deficiency leads to earlier onset and enhanced severity of experimental autoimmune encephalomyelitis. This enhanced disease in Lum-deficient mice is associated with increased production of IL-17 and IL-21 and decreased TH17 cell apoptosis. Dysregulation in cytokine production appears to be specific to TH17 cells as TH1 and TH2 cell polarization and/or cytokine production were unaltered. Furthermore, adoptive transfer of MOG-specific TH17 cells derived from Lum-deficient mice led to earlier onset and increased severity of disease compared to controls highlighting a TH17 cell-intrinsic effect of Lum. Taken together, our results suggest that Lum negatively regulates encephalitic TH17 cells, implicating a potential therapeutic pathway in TH17 cell-mediated autoimmune and inflammatory diseases.

Resveratrol (RSV, trans-3,4,5-Trihydroxystilbene), a type of polyphenol originally found in red wines, shows a great promise for the treatment of cancer, aging, type 2 diabetes and cardiovascular diseases. Recent studies suggest that suppressing the signaling pathway mediated by mTOR, a well-known energy sensor that integrates various hormonal, nutrient and environmental signals to regulate cell growth, metabolism and survival, could play an important role in mediating the beneficial effect of RSV. The underlying mechanisms by which RSV inhibits mTOR signaling remain elusive, but our recent studies show that RSV inhibits amino acid-stimulated mTOR signaling in C2C12 fibroblasts via a Sirt1- and AMPK-independent mechanism. RSV treatment has no effect on the expression levels of mTOR, raptor and DEPTOR, but greatly promotes the interaction between mTOR and its inhibitor DEPTOR. Our results reveal a novel mechanism by which RSV inhibits mTOR signaling and its function.

Adiponectin, which exists in serum in three major complexes including trimer, hexamer, and the high molecular weight (HMW) form, has strong insulin sensitizing, anti-inflammatory and anti-diabetic functions. Different adiponectin complexes exert tissue-specific biological functions and activate distinct signaling pathways. In this review, we summarize our current understanding on the mechanisms regulating adiponectin multimerization. We also describe the major target tissues in which distinct adiponectin multimers exert their functional roles. Finally, we discuss the potential involvement of endoplasmic reticulum stress and mitochondrial stress in diet-induced adiponectin downregulation and highlight the roles of Disulfide bond A oxidoreductase-like protein (DsbA-L) in the prevention of endoplasmic reticulum stress and promotion of adiponectin multimerization, stability, and function.

Background and purpose To evaluate long-term outcome in locoregionally advanced nasopharyngeal carcinoma (NPC) treated with intensity-modulated radiation therapy (IMRT) and concurrent chemotherapy. Material and methods Between January 2006 and August 2008, 249 patients with stage III–IVb NPC were treated by IMRT plus concurrent chemotherapy in this multicenter prospective study. Results With a mean follow-up of 54.1 months, the 5-year actuarial rates of overall survival (OS), local recurrence-free survival (LRFS), regional recurrence-free survival (RRFS), and distant metastasis-free survival (DMFS) were 78.4%, 86.8%, 88.4%, 78.0%, respectively. There were 29 local recurrences, 25 regional recurrences and 52 distant metastases, respectively. Distant metastasis is the main cause of treatment failure. N-stage was an independent prognostic factor for LRFS, RRFS, DMFS and OS. Acute toxicity ⩾grade III mainly consisted of mucositis (34.9%), neutropenia (11.2%), xerostomia (5.6%), and dermatitis (5.2%). The main documented late toxicity was xerostomia, and the severity of xerostomia decreased over time. At 24 months after treatment, 13.2% of patients had grade 2 xerostomia, and none had grade 3 or 4 xerostomia. Conclusions IMRT with concurrent cisplatin chemotherapy resulted in encouraging rates of local and distant control and overall survival with acceptable rates of acute and limited rates of late toxicity in patients with locoregionally advanced NPC. Distant metastasis remained the main cause of failure. More effective systemic therapy should be explored for patients with advanced N-stage.

SummaryIdentification of key regulators of lipid metabolism and thermogenic functions has important therapeutic implications for the current obesity and diabetes epidemic. Here, we show that Grb10, a direct substrate of mechanistic/mammalian target of rapamycin (mTOR), is expressed highly in brown adipose tissue, and its expression in white adipose tissue is markedly induced by cold exposure. In adipocytes, mTOR-mediated phosphorylation at Ser501/503 switches the binding preference of Grb10 from the insulin receptor to raptor, leading to the dissociation of raptor from mTOR and downregulation of mTOR complex 1 (mTORC1) signaling. Fat-specific disruption of Grb10 increased mTORC1 signaling in adipose tissues, suppressed lipolysis, and reduced thermogenic function. The effects of Grb10 deficiency on lipolysis and thermogenesis were diminished by rapamycin administration in vivo. Our study has uncovered a unique feedback mechanism regulating mTORC1 signaling in adipose tissues and identified Grb10 as a key regulator of adiposity, thermogenesis, and energy expenditure.

APPL1 is an adaptor protein that plays a critical role in regulating adiponectin and insulin signaling. However, how APPL1 is regulated under normal and pathological conditions remains largely unknown. In this study, we show that APPL1 undergoes phosphorylation at Ser(430) and that this phosphorylation is enhanced in the liver of obese mice displaying insulin resistance. In cultured mouse hepatocytes, APPL1 phosphorylation at Ser(430) is stimulated by phorbol 12-myristate 13-acetate, an activator of classic PKC isoforms, and by the endoplasmic reticulum (ER) stress inducer, thapsigargin. Overexpression of wild-type but not dominant negative PKCα increases APPL1 phosphorylation at Ser(430) in mouse hepatocytes. In addition, suppressing PKCα expression by shRNA in hepatocytes reduces ER stress-induced APPL1 phosphorylation at Ser(430) as well as the inhibitory effect of ER stress on insulin-stimulated Akt phosphorylation. Consistent with a negative regulatory role of APPL1 phosphorylation at Ser(430) in insulin signaling, overexpression of APPL1(S430D) but not APPL1(S430A) impairs the potentiating effect of APPL1 on insulin-stimulated Akt phosphorylation at Thr(308). Taken together, our results identify APPL1 as a novel target in ER stress-induced insulin resistance and PKCα as the kinase mediating ER stress-induced phosphorylation of APPL1 at Ser(430).

Using high-speed visual equipment is an effective method to locate mobile targets. Under the circumstance of high sensitivity(500Hz), except for the Gaussian noise, atmospheric instability has also an important impact on the image quality. To solve the problem, a method is proposed in this paper based on image power spectrum to analyze and evaluate the Gaussian noise, atmospheric noise meanwhile combined with wavelet denoising to remove the noise aiming at the images acquired by DALSA's 8192*32 high-sensitivity camera. Firstly, image databases are established based on the outdoor working conditions, including normal images, Gaussian noise images loaded with different simulated characteristics and atmospheric noise images in different simulated frequencies. Power spectrum ratio of all the images in the databases is calculated, and the image power spectrum critical value is determined. Then the evaluation and classification of the image noise is got according to the databases and the threshold. wavelet denoising is introduced to remove the noise subsequently. Finally, the comparison of power spectrum between the image untreated and treated is made to evaluate the effect of the method above. Experimental results show that the way can evaluate and remove the noise of image effectively for high-speed visual images.

Adiponectin has been receiving a great deal of attention due to its potential therapeutic use for metabolic and cardiovascular disorders. Adiponectin expression levels and multimerization are down-regulated in obesity and up-regulated by insulin sensitizers such as thiazolidinediones (TZDs), metformin, sulfonylurea and resveratrol (RSV). The precise mechanisms underlying adiponectin up- and down-regulation remain largely unknown, but recent studies indicate that the cellular and plasma levels of adiponectin could be regulated at both transcriptional and post-transcriptional levels. At the post-translational level, TZDs and resveratrol promote adiponectin levels and multimerization via up-regulation of disulfide-bond-A oxidoreductase-like protein (DsbA-L). Adiponectin levels are also stimulated by FOXO1 and AMP-activated protein kinase (AMPK), and are suppressed by PKA or silencing mediator of retinoid and thyroid hormone receptors (SMRT). Since multimerization is important not only for adiponectin function but also for stability, increasing adiponectin multimerization has become a promising drug target for the treatment of metabolic diseases and other related disorders.

Coordinate measuring system which based on the theory of binocular stereo vision is widely used in many areas, whereas their effective measuring ranges are usually not larger than ten meters. In modern times the surveillance of large scale is used more and more in the civil and military area with the development of camera and computer technology. So based on this requirement this paper developed a new measuring model for this binocular stereo vision measuring system which is proper used in outdoor surveillance to get the 3D coordinate of the moving object. When the distance between two cameras is hundreds meters, the installation and camera calibration are quiet simple and convenient without expensive calibration apparatus and an elaborate setup or a planar pattern shown at a few different orientations or complicated camera imaging model and the parameters of math model are easy to get. After building the model of measuring system error analysis is performed to show influence of every parameter on the measuring system error. Both computer simulation and real data have been used to test the validity of our new simple measuring system model.

In order to measure accurately position of object in remote large area with vision measurement method, a special layout of vision measurement system was designed. Some calibration objects which have the same height with cameras were placed at specific position in the measurement area. By adjusting carefully the posture of cameras, those calibration objects were imaged at the same pixel row which contained the camera principal point. As a result, the master planes of cameras which were determined by the pixel row which contained the principal point and the optical axis of cameras were horizontal and coplanar. So, the u pixel coordinate of any space point was same with the one of its projection point in the xoy coordinate plane and a simplified calculation model was derived. Compared with the traditional vision measurement method, this method is simpler and more suitable for large remote area measurement. In order to verify the validity of this method, a verification experiment had been made. The experimental results show that the resolving accuracy of this model is very high, and this measurement method is very good to meet the requirement of large remote area measurement.

In order to measure precisely the position of remote falling point in large-range using stereo vision measurement technology, a fast accurate installation method with calibration objects for the field vision measurement system was proposed. The first thing of this method was to calculate accurately parameters of system based on measurement conditions. Then, some special calibration objects were emplaced with the same height along the theoretical main axis of camera. According to the images of calibration objects, the pose of camera was adjusted carefully to complete the fast accurate installation of camera. Simulation and experimental results show that this method is very suitable for locating the remote falling point in large-scale field. And the measurement accuracy of this method is about 0.3%.

Far-range photogrammetry is widely used in the location determination problem in some dangerous situation. In this paper we discussed the camera calibration problem which can be used in outdoors. Location determination based on stereo vision sensors requires the knowledge of the camera parameters, such as camera position, orientation, lens distortion, focal length etc. with high precision. Most of the existed method of camera calibration is placing many land markers whose position is known accurately. But due to large distance and other practical problems we can not place the land markers with high precision. This paper shows that if we don't know the position of the land marker, we also can get the extrinsic camera parameters with essential matrix. The real parameters of the camera and the computed parameters of the camera give rise to the geometric error. We develop and present theoretical analysis of the geometric error and how to get the extrinsic camera parameters with high precision in large scale measurement. Experimental results of the project which is used to measure the drop point of a high speed object testify the method we proposed with high precision compared with traditional methods.

The natural polyphenol resveratrol (RSV) displays a wide spectrum of health beneficial activities, yet the precise mechanisms remain to be fully elucidated. Here we show that RSV promotes the multimerization and cellular levels of adiponectin in 3T3-L1 adipocytes. The stimulatory effect of RSV was not affected by knocking out Sirt1, but was diminished by suppressing the expression levels of DsbA-L, a recently identified adiponectin-interactive protein that promotes adiponectin multimerization. Suppression of the Akt signaling pathway resulted in an increase in the expression levels of DsbA-L and adiponectin. On the other hand, knocking out FOXO1 or suppressing the activity or expression levels of the AMP-activated protein kinase (AMPK) down-regulated DsbA-L and adiponectin. The stimulatory effect of RSV on adiponectin and DsbA-L expression was completely diminished in FOXO1-suppressed and AMPK-inactivated 3T3-L1 adipocytes. Taken together, our results demonstrate that RSV promotes adiponectin multimerization in 3T3-L1 adipocytes via a Sirt1-independent mechanism. In addition, we show that the stimulatory effect of RSV is regulated by both the Akt/FOXO1 and the AMPK signaling pathways. Last, we show that DsbA-L plays a critical role in the promoting effect of RSV on adiponectin multimerization and cellular levels.

On the basis of summarizing the methods of camera calibration, a new method of direct geometric solution (DGS) based on calibration objects is proposed. It is required to establish some beacons in particular position with equal height in measurement area. Adjust camera carefully to ensure that the measurement plane is orthogonal to the image plane of the camera. The DGS model which is used to calculate the coordinates of points in measurement area is built with the pinhole imaging model and the coordinates of calibration objects. The comprehensive error caused by measurement error was simulated in MATLAB with DGS model, and the experiment of actual measurement was done. The experiment data was processed respectively by the DGS model and the DLT (direct linear transformation) model. The results show obviously that both the accuracy and error stability of DGS are better than ones of DLT. The average relative error of DGS in this experiment is 0.5%. The DGS model is better than the DLT model in locating the impact point in remote large area.

In position measurements by far-range photogrammetry, the scale between object and image has to be calibrated. It means to get the parameters of the perspective projection matrix. Because the image sensor of fast-camera is CMOS, there are many uncertain distortion factors. It is hard to describe the scale between object and image for the traditional calibration based on the mathematical model. In this paper, a new method for calibrating stereo vision systems with neural networks is described. A linear method is used for 3D position estimation and its error is corrected by neural networks. Compared with DLT (Direct Linear Transformation) and direct mapping by neural networks, the accuracy is improved. We have used this method in the drop point measurement of an object in high speed successfully.

Adiponectin is an adipose-tissue-derived hormone with anti-diabetic, anti-atherogenic and anti-inflammatory functions. Adiponectin circulates in the bloodstream in trimeric, hexameric and high-molecular-mass species, and different forms of adiponectin have been found to play distinct roles in the regulation of energy homoeostasis. The serum levels of adiponectin are negatively correlated with obesity and insulin resistance, yet the underlying mechanisms remain elusive. In the present review, we summarize recent progress made on the mechanisms regulating adiponectin gene transcription, multimerization and secretion. We also discuss the potential relevance of these studies to the development of new clinical therapy for insulin resistance, Type 2 diabetes and other obesity-related metabolic disorders.

Calorie restriction (CR) alleviates insulin resistance and has a beneficial effect on numerous metabolic disorders, yet the underlying mechanism has not been fully elucidated. In the present study, we found that CR of mice (60% of the diet consumption compared with ad libitum mice) reduces the expression levels of Grb2 in skeletal muscle, an insulin target tissue that accounts for 85% of insulin-stimulated blood glucose clearance. To determine whether Grb2 downregulation contributes to increased insulin sensitivity in the regulation of glucose metabolism, we generated C2C12 cell lines in which the expression of Grb2 is suppressed by RNA interference. Suppressing Grb2 expression in C2C12 myoblasts enhances insulin-stimulated insulin receptor substrate (IRS)-1, tyrosine phosphorylation, and Akt phosphorylation, which is associated with decreased IRS-1 serine phosphorylation at residues 307, 612, and 636/639. In addition, reducing Grb2 expression levels increased insulin-stimulated glucose uptake in C2C12 myotubes. Reduced IRS-1 serine phosphorylation is also found in Grb2+/− heterozygous knockout mice, which is associated with enhanced insulin signaling and resistance to high-fat diet-induced glucose and insulin intolerance. All together, our results suggested that reducing the expression levels of Grb2 provides a mechanism by which CR increases insulin sensitivity in vivo.

Clinical, epidemiological, and biochemical studies have highlighted the role of obesity-induced insulin resistance in various metabolic diseases. However, the underlying molecular mechanisms remain to be established. In the present study, we show that palmitate-induced serine phosphorylation of phosphoinositide-dependent protein kinase-1 (PDK1) negatively regulates insulin signaling. PDK1-mediated Akt phosphorylation at Thr308 in the activation loop is reduced in C2C12 myotubes treated with palmitate or overexpressing protein kinase C theta (PKCtheta), a kinase that has been implicated in hyperlipidemia-induced insulin resistance. Palmitate treatment also inhibited platelet-derived growth factor-stimulated Akt phosphorylation, suggesting that the inhibition could occur at a site independent of IRS1/2. The inhibitory effect of palmitate on PDK1 and Akt was diminished in PKCtheta-deficient mouse embryonic fibroblasts (MEFs) by treating C2C12 myotubes with PKCtheta pseudosubstrates. In vivo labeling studies revealed that PDK1 undergoes palmitate-induced phosphorylation at two novel sites, Ser504 and Ser532. Replacing Ser504/532 with alanine disrupted PKCtheta-catalyzed PDK1 phosphorylation in vitro and palmitate-induced PDK1 phosphorylation in cells. PDK1-deficient MEFs transiently expressing PDK1S504A/S532A but not PDK1S504E/S532D showed increased basal and insulin-stimulated Akt phosphorylation at Thr308 when compared with MEFs expressing wild-type PDK1. Taken together, our results identify PDK1 as a novel target in free fatty acid-induced insulin resistance and PKCtheta as the kinase mediating the negative regulation.

This paper focuses on consumer fraud in auction website, and finds out why consumers may cheat sellers. At present, practitioners and scholars from home and abroad lay more emphasis on how to cope with website fraud, while less attention has been put on the consumer fraud. Based on the current situation of auction website, five key factors that have impact on consumer fraud have been put forward: consumer characteristics, e-commerce technology, virtual network, e-commerce law and punishment mechanism of faith-breaking. By means of factor analysis, four factors have been extracted, and the correlations between those factors and consumer fraud behavior have been researched.

A typical manufacture firm can be seen as a multi-echelon inventory system. This paper focuses on a multi-echelon inventory system which consists of firm warehouse and multiple distribution spots. And a model has been set forward in order to illuminate the inventory control strategies in a manufacture firm, distribution center and the retail warehouses according to certain fulfillment rate of customer requirement and production constraints of the firm resource and capacity, which aims to minimize the total inventory cost to identify the safe stock, ordering points and ordering quantities. And then simulation software Witness has been used to explain how to optimize multi-echelon inventory of a manufacture industry based on a simplified example.

The paper focuses on the qualitative simulation of consumer behavior under the environment of electronic commerce. The foundation of the qualitative simulation system is consumer attitude theory, consumer psychology, sociology and economics, and the system is oriented to how consumer behavioral intention has been formed. According to consumer behavior characteristics, the qualitative simulation system has been designed based on qualitative simulation and qualitative process theory. The system mainly consists of three modules: consumer perceived utility, consumer attitude and consumer intention module. By Visual Basic, the system function of qualitative simulation in the competitive markets has been fulfilled in the paper.

This paper focuses on the consumer innovation diffusion behavior simulation in the environment of electronic commerce by means of Agentsheets software. Firstly, it has been discussed that the consumer innovation diffusion behavior has been influenced by such factors, which mainly consist of mass-media, human relation, marketing strategy and network safe. After the characteristics of the famous Bass diffusion model having been analyzed, the limitations of Bass model on the consumer innovation diffusion behavior simulation have been set forward. Then according to the rational supposition of the simulation of consumer innovation diffusion behavior, the latent consumers are divided into three types: innovators, imitators and laggards, and different types of consumers are influenced by mass-media, human relation and marketing strategy differently in a way. And then the simulation structure and simulation process have been designed on condition that the diffusion coefficients among three kinds of people are both uncertain and unlike. What's more, the simulation software Agentsheets has been used to carry out the simulation. Simulation results show different types of consumers are relative to different diffusion curve slopes. On the predefined variable coefficients, the curve of the consumer innovation diffusion process is similar to the "S" shape curves. If the diffusion coefficient is changed, the slope of the curve is also changed in some degree but the basic form is still the same. Finally, based on the simulation results, reference guides to electronic businesses to make relative marketing decisions have been set up.

e16054 Background: To evaluate the treatment efficacy, toxicity and prognostic factors of patients with NPC treated with IMRT. Methods: Between January 2006 and August 2008, 300 patients with pathologically diagnosed NPC from 6 medical center received IMRT. The number of patients with stage I, II, III, IVa-b disease (UICC/AJCC 2002 staging system) was 6, 45,141, and 108, respectively. The prescription doses were as follows: 70~74Gy/30 fraction to GTVnx, 68~70Gy/30 fraction to GTVnd, 60~64Gy/30 fraction to CTV1,50~54Gy /30 fraction to CTV2. Patients with stage III, IVa-b disease also received cisplatin-based chemotherapy. Results: The median follow-up time was 47.1 months (range,11-68 months). There were 18,15 and 42 patients who had developed local, regional recurrence and distant metastasis, respectively. There were 45 patients died. 34 patients died of distant metastasis,6 died of local and regional recurrence, 2 died of re-treatment, one died of hemorrhage complications of nasopharynx,one died of second primary tumor, and the other one died of unknown cause.The 4-year rate of local control (LC), regional control(RC), metastasis-free survival(DMFS),disease-free survival(DFS) and overall survival(OS) was 94.0%, 95.5%, 87.4%, 80.8%, 86.1%, respectively. Multivariate analysis showed that N stage was the only prognostic factor for OS (x2=3.912, p=0.048, HR=14.565), DMFS (x2=5.195, p=0.023, HR=8.737)and DFS (x2=7.613,p=0.006, HR=7.628), in these patients. Mucositis was the most severe acute toxicity, with 18.1% grade 1, 48.6% grade 2, 33.3% grade 3 . No patient suffered from grade 4 mucositis. Xerostomia was the most common seen late toxicity, with 8% grade 0, 50.5% grade 1, 4.6% grade 2. No grade 3-4 xerostomia was observed. Conclusions: IMRT can improve the treatment efficacy of patients with NPC. The acute and late toxicities were tolerated. Distant metastasis becomes the main treatment failure. N stage is a significant prognostic factors.